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Malus sylvestris; alleles; apples; effective population size; gene banks; genetic relationships; genotyping; haplotypes; introgression; plant genetics; pollination; seed orchards; single nucleotide polymorphism; single nucleotide polymorphism arrays; Netherlands
Abstract:
... The Netherlands’ field genebank collection of European wild apple (Malus sylvestris), consisting of 115 accessions, was studied in order to determine whether duplicates and mistakes had been introduced, and to develop a strategy to optimize the planting design of the collection as a seed orchard. We used the apple 20K Infinium single nucleotide polymorphism (SNP) array, developed in M. domestica, ...
... Although human-imposed selection is known to have altered plant traits during crop domestication, the effect of selection on host susceptibility or resistance is not well understood. Moreover, the domestication of perennial tree fruit crops, unlike annual crops, was driven by hybridization, clonal propagation, and selection of desirable phenotypes, which could confound the accurate assessment of d ...
National Center for Biotechnology Information; Rosaceae; apples; data collection; databases; genome; loci; plant genetics; single nucleotide polymorphism arrays
Abstract:
... Accurate SNP locus identifiers are essential for the correct matching and aggregation of multiple historical datasets. The National Center for Biotechnology Information (NCBI) issued unique “submitted SNP identification” numbers (ssIDs) for every published SNP submitted to its database. Discrepant ssID information was discovered for 78 SNPs for the 8K IRSC apple SNP array while attempting to colla ...
... Marker-assisted seedling selection (MASS) has much potential to improve the efficiency of traditional apple breeding. Theoretical and empirical evaluation of selection cost and theoretical evaluation of genetic gain in previous studies support this promise. However, there is a lack of empirical validation of MASS efficiency reported in tree fruit crops for genetic gain and cost nor even genetic ga ...
... Flesh browning after cutting or bruising reduces the commercial value of apple fruit. Here, we investigated the genetic factors that regulate flesh browning (at 15 min and 24 h after grating) by a genome-wide association study (GWAS) of 24 sib-families (468 genotypes). Three quantitative trait loci (QTLs) were detected on chr5, chr16, and chr17. Their effects were estimated in a germplasm collecti ...
... Malus baccata (L.) Borkh. and Malus toringo (Siebold) Siebold ex de Vriese of the genus Malus Mill. (Rosaceae) are wild crabapples occurring in temperate East Asia. Despite their horticultural importance as ornamental trees and the natural resources in apple breeding, their phylogenetic relationships have never been determined clearly owing to lack of resolution in previous studies. We characteriz ...
... In apple (Malus spp.), crown gall disease, caused by the bacterial pathogens Rhizobium radiobacter (Ti) and R. rhizogenes (Ti), can be severe. To control the disease, breeding of apple rootstocks that exhibit crown gall resistance is a promising approach. In this study, we used a full-sib F₁ population derived from a ‘JM7’ (susceptible) × Sanashi 63 (resistant) cross to identify quantitative trait ...
... The high-value apple cultivar ‘Honeycrisp’ is prone to bitter pit, a physiological disorder characterized by the development of dark, sunken lesions on fruit particularly towards the calyx-end. Identifying the genetic basis of bitter pit is critical for mitigation of the disorder and development of bitter pit-free varieties. To this end, we performed an RNA sequencing experiment to compare the pee ...
acidity; apples; cold storage; fruit acids; fruit quality; genotyping; incomplete dominance; loci; models; plant genetics; sensory evaluation; single nucleotide polymorphism arrays; sweetness
Abstract:
... Acidity and sweetness are important qualities for apple breeders and understanding their genetic regulation can improve the breeding process. In previous QTL studies, fruit quality assessments were performed using instrumental measurements, leading to the identification of two major QTL Ma and Ma3. Here, we use sensorial data to investigate the role of known and unknown genetic factors in the perc ...
... Hybridizations involving different species are often hindered because of incompatibility reactions. Although these reproductive barriers have been observed in many plant species, the underlying mechanisms remain to be comprehensively elucidated. In this study, we detected a hybridization barrier between apple (Malus × domestica) and pear (Pyrus spp.) belonging to different genera in the subtribe M ...
... Apple blue mold causes significant postharvest economic losses worldwide. A blue mold resistance locus, qM-Pe3.1, was previously identified on chromosome 3 of Malus sieversii PI 613981, a wild accession with inferior fruit quality. Introgression of the resistance allele into elite breeding germplasm is difficult and success of introgression and the effect of the PI 613981 genome on fruit quality c ...
... Apple industries suffer from major apple diseases because of widely planted susceptible cultivars. Developed disease-resistant cultivars that often carry only a single source of resistance are not expected to be durable over time. Cultivars with multiple sources of resistance are often commercially unacceptable due to unsatisfactory fruit quality alleles inherited from unimproved and improved pare ...
... Market demand for non-browning apple (Malus × domestica Borkh.) fruit is expected to increase. To facilitate development of non-browning cultivars, we investigated the origin, inheritance pattern, and causal genomic region of the non-browning trait through genetic and quantitative trait loci (QTL) analysis of two F₁ populations (165A27 and KA27) derived from crosses between the non-browning cultiv ...
Vincent G. M. Bus; Reiny W. A. Scheper; Monika Walter; Rebecca E. Campbell; Biff Kitson; Lauren Turner; Brent M. Fisher; Sarah L. Johnston; Chen Wu; Cecilia H. Deng; Gagandeep Singla; Deepa Bowatte; Linley K. Jesson; Duncan I. Hedderley; Richard K. Volz; David Chagné; Susan E. Gardiner
... Current control strategies for the major apple disease European canker (EC) are laborious and expensive, and often do not prevent progression of the disease, which can lead to loss of trees and therefore production. Hence, the development of resistant cultivars is a significant goal for breeders supporting growers in maritime climates conducive to the disease. With genetic markers increasingly bei ...
... As plant-specific transcription factors, ovate family proteins (OFP) were involved in the regulation of plant growth, development, and stress response. Although OFP proteins have been reported in some species, little is known about their evolution, structure, expression levels under biological and abiotic stress, and interactions among OFP members in apple. In this study, 26 apple MdOFP genes were ...
alleles; apples; breeding; breeding programs; cold; cold storage; computer software; cultivars; family studies; fruit acids; fruit consumption; fruits; genetic traits; genotyping; germplasm; linkage groups; loci; models; new variety; parents; phenotypic variation; quantitative trait loci; single nucleotide polymorphism; titratable acidity
Abstract:
... Acidity is a critical component of the apple fruit consumption experience. In previous biparental family studies, two large-effect acidity QTLs were reported using freshly harvested fruit. Objectives of this study were to determine the number and location of QTLs for acidity variation in a large apple breeding program and ascertain the quantitative effects and breeding relevance of QTL allelic com ...
... Reference genes are a key factor for the sensitivity and reliability of quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis. The elongation factor gene EF1α encodes a highly conserved ubiquitous protein that functions in the binding of aminoacyl-tRNA to the ribosome during peptide synthesis in eukaryotes, which is proposed for qRT-PCR as an internal reference for its pu ...
... Apple (Malus × domestica) is the third important fruit in terms of production and consumption worldwide. Apple scab caused by Venturia inaequalis is the most devastating disease of apple. In the apple-growing regions, many fungicides are sprayed to control the disease leading to increase in the production cost. Development of scab-resistant cultivars is the long-lasting solution to control the dis ...
... Strigolactones (SLs) are a recently discovered type of plant hormone that controls various developmental processes. The DWARF53 (D53) protein in rice and the SMAX1-LIKE (SMXL) family in Arabidopsis repress SL signaling. In this study, bioinformatics analyses were performed, and 236 SMXL proteins were identified in 28 sequenced plants. A phylogenetic analysis indicated that all potential SMXL prote ...
... Growth-regulating factor (GRF) proteins comprise a family of plant-specific transcription factors that play crucial roles in regulating plant architecture. However, information pertaining to the GRF gene family in apple (Malus × domestica) is limited. In the present study, 16 GRF family members were identified in the apple genome, and nine were predicted to be putative targets of apple microRNA396 ...
... The cultivated apple is susceptible to several pests including the rosy apple aphid (RAA; Dysaphis plantaginea Passerini), control of which is mainly based on chemical treatments. A few cases of resistance to aphids have been described in apple germplasm resources, laying the basis for the development of new resistant cultivars by breeding. The cultivar ‘Florina’ is resistant to RAA, and recently, ...
... Dihydrochalcones (DHCs) are a distinctive characteristic of Malus species, with phloridzin as the major DHC in most Malus species, including cultivated apple. DHCs in apple have unique chemical properties with commercial and nutritional value and may yield important insights into the evolution and physiology of apple. A few species produce sieboldin and trilobatin instead of phloridzin, and inters ...
... Valsa canker caused by the necrotrophic pathogen Valsa mali (Vm) severely affects apple production in Eastern Asia. The molecular basis underlying the apple response to Vm infection is poorly understood. Hence, we performed RNA sequencing (RNA-seq) to investigate the dynamic gene expression profiles of a major apple cultivar, ‘Changfu No.2’, during Vm infection. Compared with the control (C), 104, ...
... IGT family genes share the highly conserved motif GφL-(A/T) IGT in domain II and play an essential role in plant form. The tree architecture of apple (Malus × domestica Borkh.) affects fruit quality and yield. However, little information is available regarding IGT family genes in apple. Apple cultivars of four ideotypes (columnar, tip bearer, spur, and standard) were selected to characterize IGT f ...
... Soft scald and soggy breakdown are important postharvest physiological disorders of apple (Malus × domestica). ‘Honeycrisp’ and some of its offspring are particularly susceptible to developing these disorders. The purpose of this study was to identify molecular markers associated with high incidences of soft scald and soggy breakdown for use in marker-assisted breeding. Towards this aim, we employ ...
cultivars; protein synthesis; apples; fungal diseases of plants; host plants; linoleate 13S-lipoxygenase; signal transduction; Western blotting; beta-glucanase; proteomics; photosynthesis; Alternaria alternata; plant pathogenic fungi; pathotypes; chitinase
Abstract:
... Alternaria blotch, caused by the Alternaria alternata apple pathotype (A. alternata AP), is one of serious pathogen of apples. In order to better understand the molecular mechanisms that underlie the defense responses of apple resistance to Alternaria blotch disease, a comparative proteomic approach was applied to analyze of susceptible and resistant apple cultivars response to A. alternata AP inf ...
... The identification of molecular markers associated with economic and quality traits will help improve breeding for new apple (Malus × domestica Borkh.) cultivars. Tools such as the 8K apple SNP array developed by the RosBREED consortium allow for high-throughput genotyping of SNP polymorphisms within collections. However, genetic characterization and the identification of population stratification ...
... Pale green lethal (PGL) is a recessive genetic disorder of apple (Malus) characterized by severe chlorophyll deficiency and seedling lethality. Following germination, seedlings cannot photosynthesize and die at the cotyledon stage. We previously reported that the genetic and biochemical basis of PGL is due to a loss-of-function mutation in a gene required for the biosynthesis of phylloquinone (vit ...
... RCD1 is a member of the plant-specific SRO protein family. Several SRO genes have been functionally identified in the regulation of abiotic stresses in Arabidopsis and other plant species. However, the function of SROs is largely unknown in apple (Malus×domestica). In this study, six MdSRO-encoding genes were isolated, categorized into two types and mapped to six chromosomes. The phylogenetic anal ...
... Kazakhstan belongs to the center of origin of apple. Malus sieversii (Ledeb.) M. Roem., the ancestral progenitor of the cultivated apple is native to this region. Pressure on the natural habitats of this wild apple has been intensified due to agriculture, grazing, and urbanization in the last century. For decades, M. sieversii in Kazakhstan has been subjected to the “Red Book of the Kazakh SSR” an ...
... Characterization of apple germplasm is important for conservation management and breeding strategies. A set of 448 Malus domestica accessions, primarily of local Danish origin, were genotyped using 15 microsatellite markers. Ploidy levels were determined by flow cytometry. Special emphasis was given to pedigree reconstruction, cultivar fingerprinting and genetic clustering. A reference set of cult ...
David Chagné; Chris Kirk; Natalie How; Claire Whitworth; Cristian Fontic; Gemma Reig; Greg Sawyer; Simon Rouse; Lara Poles; Susan E. Gardiner; Satish Kumar; Richard Espley; Richard K. Volz; Michela Troggio; Ignasi Iglesias
... The red skin color desired by most apple consumers is not easy to achieve in warm climates, as the expression of MYB10, which regulates red pigmentation in apple, is influenced negatively by high temperatures. We describe the development and validation of a genetic marker for red skin coloration that effectively predicts color in a warm summer environment in Spain, as well as more temperate climat ...
... We constructed a high-density genetic linkage map of bronze loquat (Eriobotrya deflexa) by using a three-way cross of loquat (Eriobotrya japonica) × (loquat × bronze loquat) and simple sequence repeat (SSR) and random amplified polymorphic DNA (RAPD) markers. The positions of the SSR loci used in this study were previously identified on reference maps of pears (Pyrus spp.) and apples (Malus spp.). ...
... The non-expressor of pathogenesis-related genes 1 (NPR1) plays essential roles in the salicylic acid (SA) signal pathway and in systemic acquired resistance (SAR) responses. Although a genome-wide analysis of NPR1 gene family has been conducted in some plant species, little is known about these genes in apple (Malus spp.). In this study, eight NPR1 homologs were identified within the apple genome ...
... Soluble sugars and organic acids have a strong impact on the overall organoleptic quality of fruits. In this study, we report the identification of quantitative trait loci (QTLs) for individual sugars and organic acids in apple. A high density linkage map of apple was constructed using the 1536 EST-derived SNP GoldenGate genotyping platform. The linkage map consists of 601 molecular markers, inclu ...
... The development of molecular markers linked to specific traits is now routine practice, but the gap between genomics and breeding often delays their application. In the frame of the FP7 European project FruitBreedomics, apple pilot studies were designed to exploit the project’s outcomes towards the practical application of marker-assisted breeding (MAB) programs. The aim of this pilot study was to ...
Dysaphis plantaginea; apples; chromosome mapping; cultivars; fruit growing; genes; genetic markers; insect pests; linkage groups; loci; marker-assisted selection; microsatellite repeats; pest resistance; plant damage; polymerase chain reaction; single nucleotide polymorphism
Abstract:
... Rosy apple aphid (Dysaphis plantaginea), is one of the major insect pests of apple, causing serious physical and economic damage to fruit production. A dominant resistance gene Dp-fl was previously mapped at the bottom of linkage group LG8 from the cultivar ‘Florina’, linked to the SSR CH01h10. The development of additional genetic markers mapping closer to Dp-fl was needed to position the gene ac ...
... Accurate prediction of genetic potential and response to selection in breeding requires knowledge of genetic parameters for important selection traits. Data from breeding trials can be used to obtain estimates of these parameters so that predictions are directly relevant to the improvement program. Here, a factor allocation diagram was developed to describe the sampling design used to assess the q ...
Malus domestica; analysis of variance; apples; correspondence analysis; cultivars; diploidy; gene pool; genetic techniques and protocols; genetic variation; genotype; germplasm; germplasm conservation; homonym; hybridization; landraces; microsatellite repeats; triploidy; Portugal
Abstract:
... A set of 87 apple accessions, located in three Portuguese apple germplasm collections, plus eight reference cultivars, were analyzed using 16 SSRs with the aim of assessing their genetic diversity and structure and evaluating relationships among them. Among the accessions studied, 64 unique genotypes were identified, 51 diploids and 13 putative triploids, revealing 19 groups of synonyms and 4 of h ...
... The basic leucine zipper (bZIP) family is one of the largest transcription factor (TF) families in plants, which play crucial roles in plant growth and development. bZIP proteins are involved in multiple biological processes, as well as responses to various biotic/abiotic stresses. Although genome-wide analysis of the bZIP gene family has been conducted in several plant species, only few comprehen ...
... Breeding for fire blight resistance is a major goal of nearly all apple breeding programs worldwide. Resources for resistance can be found in Malus wild species accession such as Malus ×robusta 5 (Mr5) which carries the FB_MR5 CC-NBS-LRR fire blight resistance gene. To study the occurrence of FB_MR5 in other genetic resources of Malus, a total of 394 wild species accessions of three international ...
Diogo Denardi Porto; Vítor da Silveira Falavigna; Rafael Augusto Arenhart; Pâmela Perini; Vanessa Buffon; Rafael Anzanello; Henrique Pessoa dos Santos; Flávio Bello Fialho; Paulo Ricardo Dias de Oliveira; Luís Fernando Revers
... The molecular control of bud dormancy establishment and release is still not well understood, although some genes have already been demonstrated to play important roles in this process. The dormancy-associated MADS-box (DAM) genes were first identified in the peach EVERGROWING locus and are considered the main regulators of bud dormancy control. In this work, the apple (Malus × domestica Borkh.), ...
... Well-feathered apple trees are essential for commercial orchards to optimize yields. However, most cultivars do not form these sylleptic branches readily in commercial nurseries due to high apical dominance. Several treatments exist to promote their formation in the nurseries, one of which is heading. However, not all cultivars are expected to react similarly to these treatments. We studied the br ...
Malus domestica; apples; calcium; calcium signaling; cell growth; fluorescence; gene expression regulation; genes; pollen; pollen tubes; protein subunits; quantitative polymerase chain reaction; ribonucleases; two hybrid system techniques
Abstract:
... As a signaling molecule in plants and animals, calcium plays important roles in cell growth and development. A concentration gradient of calcium in the apex of tip-growing cells regulates polarized growth. In this study, we cloned the gene encoding a calcineurin B subunit protein, MdCBL5, in “Ralls Janet” apple. We found that MdCBL5 was strongly expressed in pollen and that the encoded protein con ...
... Postharvest texture change is a fundamental question for fruit growers and breeders as it drives consumer acceptability. To decipher the genetic control of fruit texture, we studied an apple segregating population over 2 years at harvest and after 2 months of cold storage. Texture complexity was dissected in quantitative traits, related to (i) sensory perception of fruit quality and (ii) fruit mec ...
... Scab, caused by the fungus Venturia inaequalis, is one of the most important diseases of apple. Although major scab resistance genes (R gene) have been widely studied, little is known about the molecular mechanisms underlying partial resistance, thought to be more durable. We used a candidate gene approach to decipher the genetic determinism of the durable partial resistance in ‘Président Roulin’, ...
... Inter-retrotransposon amplified polymorphisms (IRAP) are one of the main components of the retrotransposon-based molecular marker system, but currently have no applications in pear. In this context, new IRAP markers were developed and used to study the genetic polymorphism of pear cultivars and bud mutants. In total, eight polymorphic IRAP markers were selected in ten genotypes of pear with a wide ...
... The columnar trait in apple (Malus × domestica Borkh.) is characterized by short internodes, reduced branching, and increased spurs, which are labor-saving features useful for high-density planting. In this study, we used six segregating populations of a descendant of the columnar-type cultivar ‘Maypole’ and constructed a genetic linkage map for the columnar gene (Co). Based on the linkage map of ...
Malus baccata; USSR; Malus domestica; Malus prunifolia; Malus sieversii; apples; breeding; cultivars; genetic variation; germplasm; landraces; microsatellite repeats; wild relatives; China; Japan; North America; Western European region
Abstract:
... The Research Institute of Pomology (IP), Chinese Academy of Agricultural Sciences (CAAS) in Xingcheng, China, maintains hundreds of apple accessions that originated from around the world. We have used 16 microsatellites to assess the diversity and differentiation of 391 accessions within the IP that represent Malus × domestica (from China, Japan, former Soviet Republics, and Western countries) as ...
... TIFY transcription factors comprise a plant-specific family involved in the regulation of diverse developmental processes and responses to phytohormones. However, little is known about these genes in woody species, and so, we characterized the 30-member TIFY gene family of apple (Malus × domestica Borkh.). Synteny analysis indicated that segmental, tandem, and whole genome duplications have contri ...