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- Ow, D.W.; Calendar, R.; Thomason, L.
- United States Department of Agriculture patents 2004 no.US 6,746,870 B1
- transgenes; genetic transformation; genetic recombination; site-directed mutagenesis; eukaryotic cells; cultured cells; hamsters; bacteriophages; Schizosaccharomyces pombe; tobacco; Nicotiana tabacum; patents; USDA; United States
- ... This invention provides methods for obtaining specific and stable integration of nucleic acids into eukaryotic cells. The invention makes use of site-specific recombination systems that use prokaryotic recombinase polypeptides, such as the .PHI.C31 integrase, that can mediate recombination between the recombination sites, but not between hybrid recombination sites that are formed upon the recombin ...