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- Briggs, R.E.; Tatum, F.M.
- United States Department of Agriculture patents 2004 no.US 6,793,927 B1
- Mannheimia haemolytica; animal pathogenic bacteria; live vaccines; DNA methylation; methyltransferases; restriction endonucleases; bacterial proteins; molecular cloning; pneumonic pasteurellosis; cattle diseases; disease prevention; site-directed mutagenesis; genetic engineering; patents; nucleotide sequences; amino acid sequences; USDA; United States
- ... Methylation of DNA can be a critical step in the introduction of DNA into P. haemolytica. A methyltransferase has been isolated and molecularly cloned for this purpose. Use of the methyltransferase has allowed construction of defined, attenuated mutants for use as vaccines to protect cattle. ...