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- Tomomasa Matsuyama; Natsumi Sano; Tomokazu Takano; Takamitsu Sakai; Motoshige Yasuike; Atushi Fujiwara; Yasuhiko Kawato; Jun Kurita; Kazunori Yoshida; Yukinori Shimada; Chihaya Nakayasu
- Vaccine 2018 v.36 no.19 pp. 2643-2649
- Infectious spleen and kidney necrosis virus; Seriola dumerili; Seriola quinqueradiata; antibodies; antigens; blood serum; case studies; enzyme-linked immunosorbent assay; fish; genes; immune response; models; pathogens; prediction; protective effect; recombinant vaccines; screening; sorbents; vaccine development
- ... Predicting antigens that would be protective is crucial for the development of recombinant vaccine using genome based vaccine development, also known as reverse vaccinology. High-throughput antigen screening is effective for identifying vaccine target genes, particularly for pathogens for which minimal antigenicity data exist. Using red sea bream iridovirus (RSIV) as a research model, we developed ...
- Woo Ju Kwon; Jae Chan Choi; Suhee Hong; Young Chul Kim; Min Gyeong Jeong; Joon Gyu Min; Joon Bum Jeong; Kwang Il Kim; Hyun Do Jeong
- Vaccine 2020 v.38 no.51 pp. 8107-8115
- Infectious spleen and kidney necrosis virus; Oplegnathus fasciatus; Pagrus major; adjuvants; aluminum hydroxide; antigens; bream; cell lines; dose response; mortality; squalene; water temperature
- ... A formalin-inactivated red sea bream iridovirus (RSIV) vaccine was prepared using the culture supernatant of a persistently infected Pagrus major fin cell line (PI-PMF) with IVS-1 strain (RSIV subtype II Meglaocytivirus). Rock bream (Oplegnathus fasciatus) were injected with a high-dose, ultracentrifuged megalocytivirus vaccine (Ultra HSCMV, 7.0 × 10¹⁰ copies/mL), a high-dose supernatant of cultur ...