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You searched for: Journal name Veterinary immunology and immunopathology Remove constraint Journal name: Veterinary immunology and immunopathology Publication year rev 7984-2016 Remove constraint Publication year rev: 7984-2016 Publication Year 2016 Remove constraint Publication Year: 2016
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1. IL- 23/IL-17/G-CSF pathway is associated with granulocyte recruitment to the lung during African swine fever

Author:
Z. Karalyan; H. Voskanyan; Z. Ter-Pogossyan; D. Saroyan; E. Karalova
Source:
Veterinary immunology and immunopathology 2016 v.179 pp. 58-62
ISSN:
0165-2427
Subject:
African swine fever; blood serum; enzyme-linked immunosorbent assay; granulocyte colony-stimulating factor; inflammation; interleukin-17; interleukin-23; neutropenia; neutrophils; swine; tissues; viruses
Abstract:
... The interleukin (IL)-23/IL-17 pathway plays a crucial role in various forms of inflammation but its function in acute African swine fever (ASF) is not well understood. Thus, in this study, we aimed to find out whether IL-23/IL-17/G-CSF is released in acute ASF and what function it may have.The present study revealed that the production of IL-17 and IL-23 were significantly increased in the sera of ...
DOI:
10.1016/j.vetimm.2016.08.005

2. Acute phase protein haptoglobin as inflammatory marker in serum and synovial fluid in an equine model of arthritis

Author:
Laura Barrachina; Ana Rosa Remacha; Lourdes Soler; Natalia García; Antonio Romero; Francisco José Vázquez; Arantza Vitoria; María Ángeles Álava; Fermín Lamprave; Clementina Rodellar
Source:
Veterinary immunology and immunopathology 2016 v.182 pp. 74-78
ISSN:
0165-2427
Subject:
acute phase proteins; arthritis; blood serum; haptoglobins; horses; inflammation; models; synovial fluid
Abstract:
... Acute phase proteins are useful inflammatory markers in horses. Haptoglobin (Hp) serum level is increased in horses undergoing different inflammatory processes, including arthritis. However, Hp concentration has not been assessed in inflammatory synovial fluid (SF). The aim of the present study was to investigate the Hp response in serum and SF in horses undergoing experimentally induced arthritis ...
DOI:
10.1016/j.vetimm.2016.10.005

3. Adjuvant materials that enhance bovine γδ T cell responses

Author:
Jodi F. Hedges; Jeff Holderness; Mark A. Jutila
Source:
Veterinary immunology and immunopathology 2016 v.181 pp. 30-38
ISSN:
0165-2427
Subject:
T-lymphocytes; adjuvants; antibiotics; calves; innate immunity; livestock and meat industry; predation; respiratory tract diseases; vaccines
Abstract:
... Despite the availability of vaccines and antibiotics, viral, bacterial and parasite-induced intestinal and pulmonary diseases still cause significant losses to the livestock industry. Excepting improvements in calf survival due to predation, there have been only modest improvements in bovine calf survival since 1991. Strikingly, digestive and respiratory diseases still account for almost half of t ...
DOI:
10.1016/j.vetimm.2016.03.010
CHORUS:
10.1016/j.vetimm.2016.03.010
Chorus Open Access:
10.1016/j.vetimm.2016.03.010

4. Bovine natural antibodies in antibody-dependent bactericidal activity against Escherichia coli and Salmonella Typhimurium and risk of mastitis

Author:
S.E.C. van Altena; M.A. Peen; F.H. van der Linden; H.K. Parmentier; H.F.J. Savelkoul; E.J. Tijhaar
Source:
Veterinary immunology and immunopathology 2016 v.171 pp. 21-27
ISSN:
0165-2427
Subject:
Escherichia coli; Salmonella Typhimurium; antibacterial properties; antibodies; bacteria; complement; cows; immune system; immunoglobulin G; immunoglobulin M; keyhole limpet hemocyanin; lipopolysaccharides; lipoteichoic acids; mastitis; peptidoglycans; risk
Abstract:
... Natural antibodies (NAbs) are mostly IgM antibodies produced without antigenic stimulation and serve as a first line of defence of the immune system. As both natural and specific antibodies are present in animals, NAbs are studied by determining the IgM response to naïve antigens like keyhole limpet hemocyanin (KLH). In this study, we selected cows based on high and low anti-KLH IgM titers, reflec ...
DOI:
10.1016/j.vetimm.2016.01.009

5. Brucellosis vaccines for livestock

Author:
Zakia I. Goodwin; David W. Pascual
Source:
Veterinary immunology and immunopathology 2016 v.181 pp. 51-58
ISSN:
0165-2427
Subject:
Brucella; adjuvants; brucellosis; dairy products; disease reservoirs; epitopes; fetal death; genetic engineering; heterologous gene expression; hosts; immune response; livestock; livestock diseases; meat; social impact; sterilizing immunity; subunit vaccines; vaccination; vaccine development; wildlife
Abstract:
... Brucellosis is a livestock disease responsible for fetal loss due to abortions. Worldwide, this disease has profound economic and social impact by reducing the ability of livestock producers to provide an adequate supply of disease-free meat and dairy products. In addition to its presence in domesticated animals, brucellosis is harbored in a number of wildlife species creating new disease reservoi ...
DOI:
10.1016/j.vetimm.2016.03.011
CHORUS:
10.1016/j.vetimm.2016.03.011
Chorus Open Access:
10.1016/j.vetimm.2016.03.011

6. CC chemokine ligand 2 and CXC chemokine ligand 8 as neutrophil chemoattractant factors in canine idiopathic polyarthritis

Author:
Kohei Murakami; Shingo Maeda; Tomohiro Yonezawa; Naoaki Matsuki
Source:
Veterinary immunology and immunopathology 2016 v.182 pp. 52-58
ISSN:
0165-2427
Subject:
CCR2 receptor; antagonists; arthritis; chemoattractants; chemokine CCL2; chemokine CXCL2; chemotaxis; dogs; enzyme-linked immunosorbent assay; gene expression; humans; immunocytochemistry; interleukin-8; messenger RNA; monocytes; neutrophils; polymerase chain reaction; porosity; synovial fluid
Abstract:
... Canine idiopathic polyarthritis (IPA) is characterized by increased numbers of polymorphonuclear leukocytes (PMNs) in the synovial fluid (SF). In humans, CC chemokine ligand 2 (CCL2) and CXC chemokine ligand 8 (CXCL8) recruit monocytes and neutrophils, respectively, and are involved in various inflammatory disorders. The aim of this study was to assess the roles of these chemokines in driving PMNs ...
DOI:
10.1016/j.vetimm.2016.09.009

7. Canine placenta: A promising potential source of highly proliferative and immunomodulatory mesenchymal stromal cells?

Author:
Nathalie Saulnier; Julia Loriau; Marine Febre; Clément Robert; Rodolphe Rakic; Tancrède Bonte; Samuel Buff; Stéphane Maddens
Source:
Veterinary immunology and immunopathology 2016 v.171 pp. 47-55
ISSN:
0165-2427
Subject:
adipocytes; adipose tissue; adults; amnion; bone marrow; dogs; enzyme activity; immunomodulators; immunosuppression; indoleamine 2,3-dioxygenase; medicine; messenger RNA; phenotype; placenta; quantitative polymerase chain reaction; stromal cells; umbilical cord; veterinary medicine
Abstract:
... In veterinary medicine, therapeutic mesenchymal stromal cells (MSC) have been traditionally isolated from adult bone marrow or adipose tissue. Neonatal tissues, normally discarded at birth from all species have become an alternative source of cells for regenerative medicine in the human clinic. These cells have been described as being more primitive, proliferative and immunosuppressive than their ...
DOI:
10.1016/j.vetimm.2016.02.005

8. Capsular polysaccharide from Mycoplasma mycoides subsp. mycoides shows potential for protection against contagious bovine pleuropneumonia

Author:
Martin Mwirigi; Isabel Nkando; Moses Olum; Samuel Attah-Poku; Horace Ochanda; Emil Berberov; Andrew Potter; Volker Gerdts; Jose Perez-Casal; Hezron Wesonga; Reuben Soi; Jan Naessens
Source:
Veterinary immunology and immunopathology 2016 v.178 pp. 64-69
ISSN:
0165-2427
Subject:
Mycoplasma mycoides subsp. mycoides; adults; animal models; antibodies; cattle; contagious bovine pleuropneumonia; immunization; live vaccines; mice; ovalbumin; polysaccharides; virulence; Africa
Abstract:
... Contagious Bovine Pleuropneumonia (CBPP) is a severe respiratory disease caused by Mycoplasma mycoides subsp. mycoides (Mmm) which is widespread in Africa. The capsule polysaccharide (CPS) of Mmm is one of the few identified virulence determinants. In a previous study, immunization of mice against CPS generated antibodies, but they were not able to prevent multiplication of Mmm in this model anima ...
DOI:
10.1016/j.vetimm.2016.07.002

9. Change in peripheral blood lymphocyte count in dogs following adoptive immunotherapy using lymphokine-activated T killer cells combined with palliative tumor resection

Author:
Keiichiro Mie; Terumasa Shimada; Hideo Akiyoshi; Akiyoshi Hayashi; Fumihito Ohashi
Source:
Veterinary immunology and immunopathology 2016 v.177 pp. 58-63
ISSN:
0165-2427
Subject:
T-lymphocytes; antibodies; dogs; humans; immunotherapy; interleukins; intravenous injection; lymphocyte count; neoplasms; phenotype; resection
Abstract:
... We evaluated changes in peripheral blood lymphocyte (PBL) count in dogs following adoptive immunotherapy using lymphokine-activated T killer cells (T-LAK) in combination with surgery. Fifteen tumor-bearing dogs treated with T-LAK therapy combined with palliative resection of tumors were enrolled in the present study. T-LAK were generated from autologous peripheral blood mononuclear cells (PBMC) by ...
DOI:
10.1016/j.vetimm.2016.06.007

10. Characterization of host responses induced by Toll-like receptor ligands in chicken cecal tonsil cells

Author:
Khaled Taha-abdelaziz; Tamiru Negash Alkie; Douglas C. Hodgins; Bahram Shojadoost; Shayan Sharif
Source:
Veterinary immunology and immunopathology 2016 v.174 pp. 19-25
ISSN:
0165-2427
Subject:
Toll-like receptor 2; Toll-like receptor 4; bursa of Fabricius; cathelicidins; chickens; interferon-gamma; interleukin-13; interleukin-1beta; interleukin-6; intestines; ligands; lipopolysaccharides; mammals; microorganisms; oligodeoxyribonucleotides; pathogens; spleen; tonsils; vaccine adjuvants
Abstract:
... The innate responses of cecal tonsils against invading microorganisms are mediated by conserved pattern recognition receptors (PRRs) such as the Toll-like receptors (TLRs). TLRs expressed by mammalian and avian immune system cells have the capability to recognize pathogen-associated molecular patterns (PAMPs). Although, the role of TLR ligands in innate and adaptive responses in chickens has been ...
DOI:
10.1016/j.vetimm.2016.04.002

11. Colonization pattern of C. jejuni isolates of human and avian origin and differences in the induction of immune responses in chicken

Author:
Colin Pielsticker; Gerhard Glünder; Ye’ Htut Aung; Silke Rautenschlein
Source:
Veterinary immunology and immunopathology 2016 v.169 pp. 1-9
ISSN:
0165-2427
Subject:
Campylobacter jejuni; T-lymphocytes; antibodies; campylobacteriosis; cecum; chickens; food pathogens; gene expression; gene expression regulation; genotype; host-pathogen relationships; humans; immune response; immunomodulation; interferon-gamma; interleukin-6; messenger RNA; specific pathogen-free animals
Abstract:
... Campylobacter jejuni (C. jejuni) is the most frequently reported bacterial food-borne pathogen. Poultry is regarded to be the main reservoir for human campylobacteriosis. By some authors C. jejuni is considered as a commensal of the chicken gut, but clinical signs may be observed indicating host–pathogen interaction. Little is known about C. jejuni strain dependent differences in stimulation of th ...
DOI:
10.1016/j.vetimm.2015.11.005

12. Comparative analysis of the immunologic response induced by the Sterne 34F2 live spore Bacillus anthracis vaccine in a ruminant model

Author:
Okechukwu C. Ndumnego; Susanne M. Köhler; Jannie Crafford; Henriette van Heerden; Wolfgang Beyer
Source:
Veterinary immunology and immunopathology 2016 v.178 pp. 14-21
ISSN:
0165-2427
Subject:
Bacillus anthracis; Boer (goat breed); anthrax; antibodies; antigens; formaldehyde; goats; humoral immunity; immune response; immunoglobulin G; models; neutralization; neutralization tests; qualitative analysis; secondary immunization; spores; vaccination; vaccines
Abstract:
... The Sterne 34F2 live spore vaccine (SLSV) developed in 1937 is the most widely used veterinary vaccine against anthrax. However, literature on the immunogenicity of this vaccine in a target ruminant host is scarce. In this study, we evaluated the humoral response to the Bacillus anthracis protective antigen (rPA), a recombinant bacillus collagen-like protein of anthracis (rBclA), formaldehyde inac ...
DOI:
10.1016/j.vetimm.2016.06.005

13. Comparison of cytokine responses between dogs with sepsis and dogs with immune-mediated hemolytic anemia

Author:
Valerie Johnson; Brandy Burgess; Paul Morley; Ryan Bragg; Anne Avery; Steven Dow
Source:
Veterinary immunology and immunopathology 2016 v.180 pp. 15-20
ISSN:
0165-2427
Subject:
blood serum; cytokines; dogs; etiology; hemolytic anemia; immunotherapy; sepsis (infection)
Abstract:
... Cytokine abnormalities have been described previously in dogs with varied immune mediated and inflammatory conditions such as IMHA and sepsis. The purpose of this study was to establish references ranges for cytokine levels in dogs and to compare cytokine levels in normal dogs and dogs with two common inflammatory diseases (sepsis and IMHA). We hypothesized that cytokine response profiles in dogs ...
DOI:
10.1016/j.vetimm.2016.08.010

14. Comparison of mesenchymal stem cells and leukocytes from Large White and Göttingen Minipigs: Clues for stem cell-based immunomodulatory therapies

Author:
Verónica Álvarez; Francisco-Miguel Sánchez-Margallo; Rebeca Blázquez; Raquel Tarazona; Javier G. Casado
Source:
Veterinary immunology and immunopathology 2016 v.179 pp. 63-69
ISSN:
0165-2427
Subject:
Large White; T-lymphocytes; animal models; bone marrow; humans; immunomodulation; immunomodulators; miniature swine; monitoring; stem cells; swine; therapeutics; veterinary medicine
Abstract:
... The mesenchymal stem cells (MSCs) are one of the most promising cell types for human and veterinary use and their therapeutic effect is associated with their immunomodulatory properties. Farm animal models, such as pigs, have become a valuable tool to evaluate the safety and efficacy of adoptively transferred MSCs in the setting of veterinary medicine. In order to evaluate the immunomodulatory eff ...
DOI:
10.1016/j.vetimm.2016.08.002

15. Comparison of probiotic lactobacilli and bifidobacteria effects, immune responses and rotavirus vaccines and infection in different host species

Author:
Anastasia N. Vlasova; Sukumar Kandasamy; Kuldeep S. Chattha; Gireesh Rajashekara; Linda J. Saif
Source:
Veterinary immunology and immunopathology 2016 v.172 pp. 72-84
ISSN:
0165-2427
Subject:
Bifidobacterium; Lactobacillus; animal models; experimental design; health promotion; hosts; humans; immune response; immunomodulators; neonates; probiotics; protective effect; vaccines
Abstract:
... Different probiotic strains of Lactobacillus and Bifidobacterium genera possess significant and widely acknowledged health-promoting and immunomodulatory properties. They also provide an affordable means for prevention and treatment of various infectious, allergic and inflammatory conditions as demonstrated in numerous human and animal studies. Despite the ample evidence of protective effects of t ...
DOI:
10.1016/j.vetimm.2016.01.003
PubMed:
26809484
PubMed Central:
PMC4818210

16. Comparison of serum cytokine levels between dogs with multicentric lymphoma and healthy dogs

Author:
Jerome Calvalido; Geoffrey A. Wood; Anthony J. Mutsaers; Darren Wood; William Sears; J. Paul Woods
Source:
Veterinary immunology and immunopathology 2016 v.182 pp. 106-114
ISSN:
0165-2427
Subject:
T-lymphocytes; biomarkers; blood cell counts; blood serum; chemokine CCL2; dogs; drug therapy; erythrocytes; flow cytometry; granulocyte-macrophage colony-stimulating factor; humans; interferon-gamma; interleukin-10; interleukin-15; interleukin-18; interleukin-2; interleukin-6; interleukin-7; interleukin-8; keratinocytes; lymphoma; monocytes; necrosis; neoplasm cells; neutrophils; pathogenesis; prognosis; tumor necrosis factor-alpha
Abstract:
... In humans, multiple cytokines have been linked to the development of lymphoma, and are relevant biomarkers for response to chemotherapy and prognosis. In contrast, only a few circulating cytokines have been studied in dogs with lymphoma. We prospectively enrolled thirty-one dogs newly diagnosed with multicentric lymphoma. Immunophenotype was determined by flow cytometry in all dogs, separating the ...
DOI:
10.1016/j.vetimm.2016.10.009

17. CpG DNA facilitate the inactivated transmissible gastroenteritis virus in enhancing the local and systemic immune response of pigs via oral administration

Author:
Jian Lin; Chongzhi Tu; Chunxiao Mou; Xiaojuan Chen; Qian Yang
Source:
Veterinary immunology and immunopathology 2016 v.172 pp. 1-8
ISSN:
0165-2427
Subject:
DNA; T-lymphocytes; Transmissible gastroenteritis virus; adjuvants; blood serum; diarrhea; enteritis; immune response; immunoglobulin A; immunoglobulin G; interferon-gamma; interleukin-12; interleukin-6; oral administration; oral vaccination; small intestine; swine
Abstract:
... Transmissible gastroenteritis virus (TGEV) replicates in the small intestine and induces enteritis and watery diarrhea. Establishment of local immunity in the intestine would thus prevent TGEV transmission. CpG DNA has been reported as a promising mucosal adjuvant in some animals. The effects of oral immunization of CpG DNA together with inactivated TGEV (ITGEV) were investigated in this study. Pi ...
DOI:
10.1016/j.vetimm.2016.02.013
PubMed:
27032496
PubMed Central:
PMC7112793

18. Development of immune-diagnostic reagents to diagnose bovine tuberculosis in cattle

Author:
H. Martin Vordermeier; Gareth J. Jones; Bryce M. Buddle; R. Glyn Hewinson
Source:
Veterinary immunology and immunopathology 2016 v.181 pp. 10-14
ISSN:
0165-2427
Subject:
animal welfare; blood; bovine tuberculosis; cattle; cell-mediated immunity; issues and policy; vaccination; vaccine development
Abstract:
... Bovine tuberculosis remains a major economic and animal welfare concern worldwide. As part of control strategies, cattle vaccination is being considered. This approach, used alongside conventional control policies, also requires the development of vaccine compatible diagnostic assays to distinguish infected from vaccinated animals (DIVA). In this review we discuss recent advances in DIVA developme ...
DOI:
10.1016/j.vetimm.2016.02.003

19. Development of porcine ficolin-alpha monoclonal and polyclonal antibodies for determining the binding capacity of multiple GlcNAc-binding proteins to bacterial danger components

Author:
M. Abu Nahid; Steven J. Ross; Benjamin R. Umiker; Huapeng Li; Sunji Sugii; Latiful Bari
Source:
Veterinary immunology and immunopathology 2016 v.170 pp. 13-19
ISSN:
0165-2427
Subject:
Escherichia coli; antibacterial properties; antigens; bacterial proteins; binding capacity; blood proteins; blood serum; cattle; chickens; chromatography; cross reaction; dogs; horses; humans; innate immunity; lipopolysaccharides; lipoteichoic acids; mice; opsonins; polyclonal antibodies; rabbits; swine
Abstract:
... Ficolins are a group of oligomeric defense proteins assembled from collagen-like stalks and fibrinogen-like domains that have common biochemical specificity for N-acetyl-d-glucose amine (GlcNAc) and can function as opsonins. In this report, GlcNAc-binding protein (GBP) purified from porcine nonimmune serum was biochemically characterized as ficolin-α. Ficolin-α was used as an immunogen to generate ...
DOI:
10.1016/j.vetimm.2015.12.007

20. Differential macrophage function in Brown Swiss and Holstein Friesian cattle

Author:
Amanda Jane Gibson; Sally Woodman; Christopher Pennelegion; Robert Patterson; Emma Stuart; Naomi Hosker; Peter Siviter; Chloe Douglas; Jessica Whitehouse; Will Wilkinson; Sherri-Anne Pegg; Bernardo Villarreal-Ramos; Dirk Werling
Source:
Veterinary immunology and immunopathology 2016 v.181 pp. 15-23
ISSN:
0165-2427
Subject:
Brown Swiss; Holstein; Salmonella Typhimurium; autophagy; dairy cows; dairy industry; disease resistance; fungi; genes; infectious diseases; interleukin-1beta; macrophages; nitrogen; oxygen; pathogens; receptors; secretion; signal transduction
Abstract:
... There is strong evidence that high yielding dairy cows are extremely susceptible to infectious diseases, and that this has severe economic consequences for the dairy industry and welfare implications. Here we present preliminary functional evidence showing that the innate immune response differs between cow breeds. The ability of macrophages (MØ) to kill pathogens depends in part on oxygen-depende ...
DOI:
10.1016/j.vetimm.2016.02.018
PubMed:
26961672
PubMed Central:
PMC5145809

21. Duration of in vivo endotoxin tolerance in horses

Author:
Susan J. Holcombe; Carrie C. Jacobs; Vanessa L. Cook; Jeffery C. Gandy; Joseph G. Hauptman; Lorraine M. Sordillo
Source:
Veterinary immunology and immunopathology 2016 v.173 pp. 10-16
ISSN:
0165-2427
Subject:
blood; clinical examination; cross-over studies; endotoxemia; endotoxins; fever; gene expression; heart rate; horses; inflammation; interleukin-8; lipopolysaccharides; models; pain; respiratory rate; sepsis (infection); tumor necrosis factor-alpha
Abstract:
... Endotoxemia models are used to study mechanisms and treatments of early sepsis. Repeated endotoxin exposures induce periods of endotoxin tolerance, characterized by diminished proinflammatory responses to lipopolysaccharide (LPS) and modulated production of proinflammatory cytokines. Repeated measure designs using equine endotoxemia models are rarely performed, despite the advantages associated wi ...
DOI:
10.1016/j.vetimm.2016.03.016

22. Effect of breast feeding time on physiological, immunological and microbial parameters of weaned piglets in an intensive breeding farm

Author:
G.R. García; C.A. Dogi; G.E. Ashworth; D. Berardo; G. Godoy; L.R. Cavaglieri; A. de Moreno de LeBlanc; C.R. Greco
Source:
Veterinary immunology and immunopathology 2016 v.176 pp. 44-49
ISSN:
0165-2427
Subject:
Lactobacillus; adverse effects; anaerobes; atrophy; blood sampling; breast feeding; breeding; cecum; cortisol; early weaning; farms; goblet cells; immunoglobulin G; innate immunity; lymphocytes; microbiological quality; microorganisms; morphometry; piglets; rearing; villi
Abstract:
... The aim of this work was to study the long-lasting consequences of different weaning age on physiological, immunological and microbiological parameters of weaned piglets. Piglets were weaned at 14 days (14W) or 21 days (21W). Blood samples were taken for IgG and cortisol determination on preweaning day and at 4; 20 and 40 post-weaning days. Three animals of each group were sacrificed. Small intest ...
DOI:
10.1016/j.vetimm.2016.02.009

23. Effect of common carp (Cyprinus carpio) TLR9 overexpression on the expression of downstream interferon-associated immune factor mRNAs in epithelioma papulosum cyprini cells

Author:
Wei Xinxian; Jia Peng; Tong Guixiang; Wang Jinjin; Zheng Xiaocong; He Junqiang; Yang Xianle; Liu Hong
Source:
Veterinary immunology and immunopathology 2016 v.170 pp. 47-53
ISSN:
0165-2427
Subject:
Cyprinus carpio; Toll-like receptor 9; fish; gene expression regulation; gene overexpression; genes; interferons; messenger RNA; pathogens; plasmids; quantitative polymerase chain reaction; secretion
Abstract:
... Toll-like receptors (TLRs) are a class of pattern recognition receptors (PRRs) that recognize pathogen associated molecular patterns (PAMPs) and play an important role in the antiviral response. To determine the effect of common carp TLR9 (CcTLR9) overexpression on the expression of down-stream interferon associated immune factors in epithelioma papulosum cyprini (EPC) cells, may provide useful in ...
DOI:
10.1016/j.vetimm.2015.10.006

24. Effect of inflammatory environment on equine bone marrow derived mesenchymal stem cells immunogenicity and immunomodulatory properties

Author:
L. Barrachina; A.R. Remacha; A. Romero; F.J. Vázquez; J. Albareda; M. Prades; B. Ranera; P. Zaragoza; I. Martín-Burriel; C. Rodellar
Source:
Veterinary immunology and immunopathology 2016 v.171 pp. 57-65
ISSN:
0165-2427
Subject:
adhesion; bone marrow; cartilage; flow cytometry; fluorescence; gene expression; gene expression regulation; genes; horses; immune response; immunomodulation; immunomodulators; inducible nitric oxide synthase; inflammation; interleukin-6; joint diseases; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; stem cells; synovial fluid; tumor necrosis factor-alpha
Abstract:
... Mesenchymal stem cells (MSCs) are being investigated for the treatment of equine joint diseases because of their regenerative potential. Recently, the focus mainly has addressed to their immunomodulatory capacities. Inflammation plays a central role in joint pathologies, since the release of proinflammatory mediators to the synovial fluid (SF) leads to the activation of enzymatic degradation of th ...
DOI:
10.1016/j.vetimm.2016.02.007

25. Effects of choline treatment in concentrations of serum matrix metalloproteinases (MMPs), MMP tissue inhibitors (TIMPs) and immunoglobulins in an experimental model of canine sepsis

Author:
Meric Kocaturk; Oya Eralp-Inan; A. Tvarijonaviciute; Mehmet Cansev; M. Ozgur Ozyigit; J.J. Ceron; Zeki Yilmaz; M. Mufit Kahraman
Source:
Veterinary immunology and immunopathology 2016 v.180 pp. 9-14
ISSN:
0165-2427
Subject:
C-reactive protein; alanine transaminase; blood serum; choline; correlation; creatine kinase; creatinine; dogs; endotoxemia; enzyme-linked immunosorbent assay; immunoglobulin G; immunoglobulin M; intravenous injection; lipopolysaccharides; liver; metalloproteinases; models; pathophysiology; sepsis (infection); sodium chloride; urea nitrogen
Abstract:
... The aim of the present study was to investigate effects of intravenous (i.v.) choline treatment on serum matrix metalloproteinases (MMP), MMP tissue inhibitors (TIMP) and immunoglobulins (Igs), and to determine if there were relations between serum MMPs/TIMPs and C-reactive protein (CRP) (as a marker of the acute phase response), immunoglobulin G and M (IgG and IgM) (as a maker of the Ig responses ...
DOI:
10.1016/j.vetimm.2016.08.011

26. Effects of heat stress on the formation of splenic germinal centres and immunoglobulins in broilers infected by Clostridium perfringens type A

Author:
Atílio Sersun Calefi; Adriana de Siqueira; Lilian Bernadete Namazu; Carolina Costola-de-Souza; Bruno Bueno Takashi Honda; Antonio José Piantino Ferreira; Wanderley Moreno Quinteiro-Filho; Juliana Garcia da Silva Fonseca; João Palermo-Neto
Source:
Veterinary immunology and immunopathology 2016 v.171 pp. 38-46
ISSN:
0165-2427
Subject:
B-lymphocytes; Clostridium perfringens; antibodies; blood serum; bursa of Fabricius; chickens; corticosterone; culture media; exposure duration; heat; heat stress; immunoglobulin A; immunoglobulin M; immunoglobulin Y; immunosuppression; jejunum; mixed infection; morphometry; necrotic enteritis; pathogenesis; poultry production; spleen; vaccination
Abstract:
... Avian necrotic enteritis (NE) induced by Clostridium perfringens is a disease that affects mainly the first weeks of poultry’s life. The pathogenesis of NE is complex and involves the combination of several factors, such as co-infection with different species of coccidia, immunosuppression and stress. Stress is one of the main limiting factors in poultry production. Although several studies emphas ...
DOI:
10.1016/j.vetimm.2016.02.004

27. Effects of polyphenols including curcuminoids, resveratrol, quercetin, pterostilbene, and hydroxypterostilbene on lymphocyte pro-inflammatory cytokine production of senior horses in vitro

Author:
Melissa H. Siard; Kellie E. McMurry; Amanda A. Adams
Source:
Veterinary immunology and immunopathology 2016 v.173 pp. 50-59
ISSN:
0165-2427
Subject:
adverse effects; analysis of variance; brefeldin A; carbon dioxide; cell viability; cytotoxicity; dimethyl sulfoxide; ficoll; flow cytometry; flunixin; horses; inflammation; interferon-gamma; laminitis; mononuclear leukocytes; nonsteroidal anti-inflammatory agents; osteoarthritis; phenylbutazone; polyphenols; pterostilbene; quercetin; resveratrol; tumor necrosis factor-alpha
Abstract:
... Senior horses (aged ≥20 years) exhibit increased chronic, low-grade inflammation systemically, termed inflamm-aging. Inflammation is associated with many afflictions common to the horse, including laminitis and osteoarthritis, which are commonly treated with the non-steroidal anti-inflammatory drugs (NSAIDs) flunixin meglumine and phenylbutazone. Although these NSAIDs are effective in treating acu ...
DOI:
10.1016/j.vetimm.2016.04.001

28. Effects of prepartum stocking density on innate and adaptive leukocyte responses and serum and hair cortisol concentrations

Author:
P.R.B. Silva; K.M. Lobeck-Luchterhand; R.L.A. Cerri; D.M. Haines; M.A. Ballou; M.I. Endres; R.C. Chebel
Source:
Veterinary immunology and immunopathology 2016 v.169 pp. 39-46
ISSN:
0165-2427
Subject:
Jersey; blood serum; calving; chickens; cortisol; cows; eggs; glucose; haptoglobins; immunoglobulin G; lactation; leukocytes; milk equivalent; milk yield; ovalbumin; phagocytosis; pregnancy; stocking rate
Abstract:
... Objectives were to evaluate the effects of prepartum stocking density on innate and adaptive leukocyte responses, serum cortisol and haptoglobin concentrations and hair cortisol concentration of Jersey cows. The cows (254±3d of gestation) were balanced for parity (nulliparous vs. parous) and previous lactation projected 305-d mature equivalent milk yield and assigned to one of two treatments: 80SD ...
DOI:
10.1016/j.vetimm.2015.11.007

29. Effects of rearing environment on the gut antimicrobial responses of two broiler chicken lines

Author:
Vanessa L. Butler; Catherine A. Mowbray; Kevin Cadwell; Sherko S. Niranji; Richard Bailey; Kellie A Watson; John Ralph; Judith Hall
Source:
Veterinary immunology and immunopathology 2016 v.178 pp. 29-36
ISSN:
0165-2427
Subject:
anti-infective properties; broiler chickens; cecum; gene expression; hygiene; phylogeny; poultry diseases; rearing; risk reduction; tissues
Abstract:
... To reduce the risk of enteric disease in poultry, knowledge of how bird gut innate defences mature with age while also responding to different rearing environments is necessary. In this study the gut innate responses of two phylogenetically distinct lines of poultry raised from hatch to 35days, in conditions mimicing high hygiene (HH) and low hygiene (LH) rearing environments, were compared. Analy ...
DOI:
10.1016/j.vetimm.2016.06.004

30. Efficacy of an inactivated genotype 2b porcine epidemic diarrhea virus vaccine in neonatal piglets

Author:
Pil-Soo Baek; Hwan-Won Choi; Sunhee Lee; In-Joong Yoon; Young Ju Lee; Du Sik Lee; Seungyoon Lee; Changhee Lee
Source:
Veterinary immunology and immunopathology 2016 v.174 pp. 45-49
ISSN:
0165-2427
Subject:
Porcine epidemic diarrhea virus; body weight; diarrhea; economic impact; farrowing; feces; genotype; herds; immunity; inactivated vaccines; livestock breeding; neutralizing antibodies; piglets; pork industry; progeny; sows; survival rate; vaccination; vaccine development; viral shedding; virulence; virulent strains; viruses; weight gain; South Korea
Abstract:
... Massive outbreaks of porcine epidemic diarrhea virus (PEDV) recurred in South Korea in 2013–2014 and affected approximately 40% of the swine breeding herds across the country, incurring a tremendous financial impact on producers and consumers. Despite the nationwide use of commercially available attenuated and inactivated vaccines in South Korea, PEDV has continued to plague the domestic pork indu ...
DOI:
10.1016/j.vetimm.2016.04.009
PubMed:
27185262
PubMed Central:
PMC7126956

31. Efficacy of commercial genotype 1 porcine reproductive and respiratory syndrome virus (PRRSV) vaccine against field isolate of genotype 2 PRRSV

Author:
Seong-sik Ko; Sang-won Seo; Sun-young Sunwoo; Sung J. Yoo; Myung-hyee Kim; Young S. Lyoo
Source:
Veterinary immunology and immunopathology 2016 v.172 pp. 43-49
ISSN:
0165-2427
Subject:
Porcine reproductive and respiratory syndrome virus; antibody affinity; cross immunity; genotype; neutralizing antibodies; piglets; vaccination; vaccines; viral load; viremia; viruses
Abstract:
... Although several recent studies have found that type 1 porcine reproductive and respiratory syndrome virus (PRRSV) modified live virus (MLV) vaccine showed appreciable levels of cross-protection against type 2 PRRSV infection, the possibility of cross-protection between two genotype of PRRSV is still controversial. To determine potential protective efficacy against hetero-genotype field strain of ...
DOI:
10.1016/j.vetimm.2016.02.016

32. Equine herpesvirus-1 infection disrupts interferon regulatory factor-3 (IRF-3) signaling pathways in equine endothelial cells

Author:
Sanjay Sarkar; Udeni B.R. Balasuriya; David W. Horohov; Thomas M. Chambers
Source:
Veterinary immunology and immunopathology 2016 v.173 pp. 1-9
ISSN:
0165-2427
Subject:
transcription factor NF-kappa B; Equid alphaherpesvirus 1; respiratory mucosa; immune evasion; endothelial cells; nervous system diseases; respiratory tract diseases; signal transduction; pathogens; genes; paralysis; blood vessels; spinal cord; death; interferon regulatory factor-3; uterus; horses; interferons
Abstract:
... Equine herpesvirus-1 (EHV-1) is a major respiratory viral pathogen of horses, causing upper respiratory tract disease, abortion, neonatal death, and neurological disease that may lead to paralysis and death. EHV-1 replicates initially in the respiratory epithelium and then spreads systemically to endothelial cells lining the small blood vessels in the uterus and spinal cord leading to abortion and ...
DOI:
10.1016/j.vetimm.2016.03.009

33. Evaluation of CD25-positive cells in relation to the subtypes and prognoses in various lymphoid tumours in dogs

Author:
Noriyuki Mizutani; Yuko Goto-Koshino; Masaya Tsuboi; Yumiko Kagawa; Koichi Ohno; Kazuyuki Uchida; Hajime Tsujimoto
Source:
Veterinary immunology and immunopathology 2016 v.173 pp. 39-43
ISSN:
0165-2427
Subject:
B-lymphocytes; dogs; flow cytometry; humans; immunohistochemistry; interleukin-2; leukemia; lymph nodes; lymphoma; neoplasm cells; prognosis; therapeutics; tissues
Abstract:
... Interleukin-2 receptor alpha chain (CD25) expression has been reported in human lymphoid tumours and suggested to correlate with the prognosis. In this study, we detected CD25-positive cells in various types of lymphoid tumours in dogs. Immunohistochemical analyses of the tissues from diffuse large B-cell lymphoma (DLBCL) (n=6), T-zone lymphoma (TZL) (n=5), and follicular lymphoma (FL) (n=2) revea ...
DOI:
10.1016/j.vetimm.2016.03.018

34. Evaluation of leptin receptor expression on buffalo leukocytes

Author:
Giovanna De Matteis; Francesco Grandoni; Maria Carmela Scatà; Angela Catizone; Anna Reale; Alessandra Crisà; Bianca Moioli
Source:
Veterinary immunology and immunopathology 2016 v.177 pp. 16-23
ISSN:
0165-2427
Subject:
B-lymphocytes; buffaloes; confocal microscopy; energy expenditure; energy intake; flow cytometry; gene expression; immune response; leptin; leptin receptors; monocytes; natural killer cells; reverse transcriptase polymerase chain reaction
Abstract:
... Experimental evidences support a direct role for leptin in immunity. Besides controlling food intake and energy expenditure, leptin was reported to be involved in the regulation of the immune system in ruminants. The aim of this work was to highlight the expression of leptin receptor (LEPR) on Bubalus bubalis immune cells using a multi-approach assessment: flow cytometry, confocal microscopy and g ...
DOI:
10.1016/j.vetimm.2016.05.009

35. Evaluation of the effects of dexamethasone-induced stress on levels of natural antibodies in immunized laying hens

Author:
Stefano Cecchini; Michele Rossetti; Francesco Di Tomaso; Anna Rocchina Caputo
Source:
Veterinary immunology and immunopathology 2016 v.177 pp. 35-41
ISSN:
0165-2427
Subject:
antibodies; antigens; bovine serum albumin; dexamethasone; innate immunity; laying hens; pathogens; statistical analysis
Abstract:
... Natural antibodies (NAb) are an important humoral component of innate immunity, playing a pivotal role as first line of defence against pathogens even without prior antigen-specific activation or antigen-driven selection.The levels of NAb in plasma of young laying hens were explored in more detail and identified 2,4,6-trinitrophenyl bovine serum albumin (TNP-BSA), as the non-self antigen showing t ...
DOI:
10.1016/j.vetimm.2016.06.002

36. Evidence of a pro-apoptotic effect of specific antibodies in a bovine macrophage model of infection with Mycobacterium avium subsp. paratuberculosis

Author:
Ana Jolly; Silvina Lompardía; Silvia E. Hajos; Silvia L. Mundo
Source:
Veterinary immunology and immunopathology 2016 v.169 pp. 47-53
ISSN:
0165-2427
Subject:
Mycobacterium avium subsp. paratuberculosis; acridine orange; antibodies; antigens; antiserum; apoptosis; blood serum; cattle; enteritis; ethidium; flow cytometry; immune response; macrophages; microscopy; models; paratuberculosis; pathogenesis; secretion; tumor necrosis factor-alpha; vaccine development
Abstract:
... Mycobacterium avium subspecies paratuberculosis (MAP) is the causative agent of Johne's disease (JD), a chronic granulomatous enteritis in ruminants. Understanding the protective immune response following infection is crucial to improve the diagnosis and the development of vaccines against this disease. The goal of this work was to assess whether specific antibodies were able to modulate the macro ...
DOI:
10.1016/j.vetimm.2015.12.001

37. Expanding specificity of class I restricted CD8+ T cells for viral epitopes following multiple inoculations of swine with a human adenovirus vectored foot-and-mouth disease virus (FMDV) vaccine

Author:
Lasse E. Pedersen; Jared R. Patch; Mary Kenney; Raisa A. Glabman; Morten Nielsen; Gregers Jungersen; Soren Buus; William T. Golde
Source:
Veterinary immunology and immunopathology 2016 v.181 pp. 59-67
ISSN:
0165-2427
Subject:
epitopes; blood serum; staining; humoral immunity; antibodies; vaccines; major histocompatibility complex; structural proteins; Human mastadenovirus C; viruses; immune response; Foot-and-mouth disease virus; CD8-positive T-lymphocytes; swine
Abstract:
... The immune response to the highly acute foot-and-mouth disease virus (FMDV) is routinely reported as a measure of serum antibody. However, a critical effector function of immune responses combating viral infection of mammals is the cytotoxic T lymphocyte (CTL) response mediated by virus specific CD8 expressing T cells. This immune mechanism arrests viral spread by killing virus infected cells befo ...
DOI:
10.1016/j.vetimm.2016.07.012
CHORUS:
10.1016/j.vetimm.2016.07.012
Chorus Open Access:
10.1016/j.vetimm.2016.07.012

38. Experimental evaluation of inactivated and live attenuated vaccines against Mycoplasma mycoides subsp. mycoides

Author:
Martin Mwirigi; Isabel Nkando; Racheal Aye; Reuben Soi; Horace Ochanda; Emil Berberov; Andrew Potter; Volker Gerdts; Jose Perez-Casal; Jan Naessens; Hezron Wesonga
Source:
Veterinary immunology and immunopathology 2016 v.169 pp. 63-67
ISSN:
0165-2427
Subject:
Mycoplasma; Mycoplasma mycoides subsp. mycoides; adjuvants; adverse effects; contagious bovine pleuropneumonia; control methods; live vaccines; vaccination; Africa
Abstract:
... The current control method for contagious bovine pleuropneumonia (CBPP) in Africa is vaccination with a live, attenuated strain of Mycoplasma mycoides subsp. mycoides (Mmm). However, this method is not very efficient and often causes serious adverse reactions. Several studies have attempted to induce protection using inactivated mycoplasma, but with widely contradictory results. Therefore, we comp ...
DOI:
10.1016/j.vetimm.2015.12.006

39. Expression analysis of cytosolic DNA-sensing pathway genes in the intestinal mucosal layer of necrotic enteritis-induced chicken

Author:
Deivendran Rengaraj; Anh Duc Truong; Sung-Hyen Lee; Hyun S. Lillehoj; Yeong Ho Hong
Source:
Veterinary immunology and immunopathology 2016 v.170 pp. 1-12
ISSN:
0165-2427
Subject:
DNA; Marek disease; antibiotics; avian leukosis; chickens; gene expression; gene expression regulation; genes; immune response; immune system; intestines; necrotic enteritis; pathogens; poultry industry; quantitative polymerase chain reaction; sequence analysis; signal transduction
Abstract:
... Necrotic enteritis (NE) is a serious problem to the poultry farms, which report NE outbreaks more than once per year, as a result of the inappropriate use of antibiotics in the feed. The NE affected bird die rapidly as a result of various pathophysiological complications in the intestine and immune system. Also, several studies have reported that the genes exclusively related to intestine and immu ...
Handle:
10113/62616
DOI:
10.1016/j.vetimm.2015.12.010

40. Feeding a high dosage of zinc oxide affects suppressor of cytokine gene expression in Salmonella Typhimurium infected piglets

Author:
Jasper N. Schulte; Gudrun A. Brockmann; Susanne Kreuzer-Redmer
Source:
Veterinary immunology and immunopathology 2016 v.178 pp. 10-13
ISSN:
0165-2427
Subject:
Salmonella Typhimurium; cytokines; diet; gene expression; gene expression regulation; genes; immune response; lymph nodes; models; piglets; proteins; weaning; zinc; zinc oxide
Abstract:
... Suppressor of cytokine signaling (SOCS) proteins play an important role in the regulation of the immune response by inhibiting cytokines. Here we investigated the effects of zinc oxide fed at three different dosages (LZN=57ppm, MZN=167ppm, HZN=2425ppm) to weaned piglets that were or were not orally infected with Salmonella enterica serovar Typhimurium DT 104. We detected higher expression of SOCS3 ...
DOI:
10.1016/j.vetimm.2016.06.009

41. Field application of immunoassays for the detection of Mycobacterium bovis infection in the African buffalo (Syncerus caffer)

Author:
E.M.D.L. van der Heijden; A.O. Jenkins; D.V. Cooper; V.P.M.G. Rutten; A.L. Michel
Source:
Veterinary immunology and immunopathology 2016 v.169 pp. 68-73
ISSN:
0165-2427
Subject:
Mycobacterium bovis; Syncerus caffer; bovine tuberculosis; buffaloes; cell-mediated immunity; enzyme-linked immunosorbent assay; humoral immunity; interferon-gamma; tuberculin; wildlife; South Africa
Abstract:
... The African buffalo (Syncerus caffer) is considered the most important maintenance host of bovine tuberculosis (BTB) in wildlife in Southern Africa. The diagnosis of Mycobacterium bovis infection in this species mostly relies on the single intradermal comparative tuberculin test (SICTT). As an alternative, the BOVIGAM® 1G, an interferon-gamma (IFN-γ) release assay, is frequently used. The test per ...
DOI:
10.1016/j.vetimm.2015.12.003

42. Generation of potent porcine monocyte-derived dendritic cells (MoDCs) by modified culture protocol

Author:
Teerawut Nedumpun; Patcharee Ritprajak; Sanipa Suradhat
Source:
Veterinary immunology and immunopathology 2016 v.182 pp. 63-68
ISSN:
0165-2427
Subject:
antigens; dendritic cells; immunotherapy; interferon-gamma; isolation techniques; monocytes; phenotype; swine; vaccines
Abstract:
... In vitro derivation of dendritic cells (DCs) is an alternative approach to overcome the low frequency of primary DCs and the difficulty of isolation techniques for studying DC immunobiology. To date, the conventional culture protocol of porcine monocyte-derived DCs (MoDCs) has been widely used. However, this protocol is not practical due to the requirement of a substantial number of blood monocyte ...
DOI:
10.1016/j.vetimm.2016.10.002

43. Gilthead seabream (Sparus aurata) Mx gene promoters respond differentially to IPNV and VHSV infections in RTG-2 cells

Author:
J.A González-Mariscal; M.A. Fernández-Trujillo; M.C Alonso; E. García-Rosado; M.C. Álvarez; J. Béjar
Source:
Veterinary immunology and immunopathology 2016 v.171 pp. 73-80
ISSN:
0165-2427
Subject:
Infectious pancreatic necrosis virus; Sparus aurata; Viral hemorrhagic septicemia virus; antiviral properties; carrier state; genes; host-pathogen relationships; interferons; luciferase; viruses
Abstract:
... The understanding of virus–host interactions relies on the knowledge of the regulatory mechanisms of the type I interferon (IFN I)-stimulated genes (ISGs). Among ISGs, those coding Mx proteins play a main role due to their direct antiviral activity. The study of these genes in gilthead seabream is interesting, since this species displays high natural resistance to viral diseases, being asymptomati ...
DOI:
10.1016/j.vetimm.2016.02.006

44. Identification of linear B-cell epitopes on goose parvovirus non-structural protein

Author:
Tian-fei Yu; Bo Ma; Jun-wei Wang
Source:
Veterinary immunology and immunopathology 2016 v.179 pp. 85-88
ISSN:
0165-2427
Subject:
Anser anser; Anseriform dependoparvovirus 1; B-lymphocytes; Cairina moschata; Escherichia coli; Western blotting; ducklings; epitopes; farming systems; financial economics; goslings; humoral immunity; immunoassays; peptides; viral nonstructural proteins
Abstract:
... Goose parvovirus (GPV) infection can cause a highly contagious and lethal disease in goslings and muscovy ducklings which is widespread in all major goose (Anser anser) and Muscovy duck (Cairina moschata) farming countries, leading to a huge economic loss. Humoral immune responses play a major role in GPV immune protection during GPV infection. However, it is still unknown for the localization and ...
DOI:
10.1016/j.vetimm.2016.08.009

45. Identification of three novel B-cell epitopes of VMH protein from Vibrio mimicus by screening a phage display peptide library

Author:
Ning Xiao; Ji Cao; Hao Zhou; Shu-Quan Ding; Ling-Yan Kong; Jin-Nian Li
Source:
Veterinary immunology and immunopathology 2016 v.182 pp. 22-28
ISSN:
0165-2427
Subject:
B-lymphocytes; Vibrio mimicus; Western blotting; antibodies; ascites; bacteriophages; clones; consensus sequence; enzyme-linked immunosorbent assay; epitopes; fish; fish diseases; peptide libraries; rabbits; screening; sequence analysis; synthetic peptides; vaccines
Abstract:
... Vibrio mimicus is the causative agent of ascites disease in fish. The heat-labile hemolytic toxin designated VMH is an immunoprotective antigen of V. mimicus. However, its epitopes have not been well characterized. Here, a commercially available phage displayed 12-mer peptide library was used to screen epitopes of VMH protein using polyclonal rabbit anti-rVMH protein antibodies, and then five posi ...
DOI:
10.1016/j.vetimm.2016.09.005

46. IgE reactivity to hen egg white allergens in dogs with cutaneous adverse food reactions

Author:
Hidekatsu Shimakura; Jumpei Uchiyama; Taku Saito; Kazuki Miyaji; Masato Fujimura; Kenichi Masuda; Noriaki Okamoto; Douglas J. DeBoer; Masahiro Sakaguchi
Source:
Veterinary immunology and immunopathology 2016 v.177 pp. 52-57
ISSN:
0165-2427
Subject:
allergens; blood serum; dogs; egg albumen; enzyme-linked immunosorbent assay; hens; humans; immunoglobulin E; lysozyme; ovalbumin; ovomucin
Abstract:
... Dogs with cutaneous adverse food reactions (CAFR) often have specific IgE to food allergens. Egg white, which is majorly composed of ovomucoid, ovalbumin, ovotransferrin, and lysozyme, is a food allergen in dogs. Information of the IgE reactivity to purified egg white allergens supports accurate diagnosis and efficiency treatment in humans. However, to the best of our knowledge, there have been no ...
DOI:
10.1016/j.vetimm.2016.06.003

47. Immune response of turkey poults exposed at 1 day of age to either attenuated or wild Salmonella strains

Author:
Martina Hesse; Andreas Stamm; Rita Weber; Gerhard Glünder; Angela Berndt
Source:
Veterinary immunology and immunopathology 2016 v.174 pp. 1-10
ISSN:
0165-2427
Subject:
European Union; Salmonella Enteritidis; antibody formation; bacteria; cecum; chemokines; chickens; ducks; eggs; humans; immunohistochemistry; inducible nitric oxide synthase; interferon-gamma; live vaccines; mucosa; poultry meat; poults; proteins; salmonellosis; serotypes; turkeys; vaccination; virulence; virulent strains; zoonoses
Abstract:
... Salmonellosis is a foodborne zoonosis that is most often acquired by consuming poultry products such as eggs and poultry meat. Amongst other measures the vaccination of food-producing poultry is thought to contribute to a reduction in human salmonellosis. In the European Union (EU) in 2014 the licence of a commercially available Salmonella vaccine for chickens and ducks was extended to turkeys. In ...
DOI:
10.1016/j.vetimm.2016.04.004

48. Immunoprophylaxis in intensive farming systems: the way forward

Author:
Massimo Amadori; Cinzia Zanotti
Source:
Veterinary immunology and immunopathology 2016 v.181 pp. 2-9
ISSN:
0165-2427
Subject:
Porcine reproductive and respiratory syndrome virus; adaptive immunity; animal breeding; animals; antibiotics; antigens; environmental factors; farm management; farms; herds; hygiene; immune response; immunomodulators; inflammation; innate immunity; intensive farming; lean meat; microbiome; milk production; morbidity; probiotics; profitability; risk factors; vaccination; vaccines
Abstract:
... High levels of production in intensive farming systems are associated with increased replacement rates as a result of multifactorial diseases. The so-called “production diseases” may include low-grade infection reducing profitability without increased morbidity. Such infections are sustained by low pathogenic viral and bacterial agents which give rise to full-blown disease in association with poor ...
DOI:
10.1016/j.vetimm.2016.02.011

49. Immunoproteomic characterization of a Dermatophagoides farinae extract used in the treatment of canine atopic dermatitis

Author:
Raquel Moya; Jerónimo Carnés; Nuria Sinovas; Laura Ramió; Pilar Brazis; Anna Puigdemont
Source:
Veterinary immunology and immunopathology 2016 v.180 pp. 1-8
ISSN:
0165-2427
Subject:
Dermatophagoides farinae; absorbance; allergenicity; allergens; atopic dermatitis; breeds; densitometry; dogs; dust mites; enzyme-linked immunosorbent assay; humans; immunoglobulin E; immunoglobulin G; immunotherapy; mass spectrometry; molecular weight; patients; Iberian Peninsula
Abstract:
... Canine atopic dermatitis is a pruritic allergic skin disease. House dust mites have been identified as the main non-seasonal responsible agent. Unlike in human allergic patients, groups 1 and 2 antigens have been described as minor allergens in dogs, while groups 15 and 18 are considered the major allergens. Despite these differences, allergic dogs have traditionally been treated using extracts in ...
DOI:
10.1016/j.vetimm.2016.08.004

50. Impact of oral meloxicam and long-distance transport on cell-mediated and humoral immune responses in feedlot steers receiving modified live BVDV booster vaccination on arrival

Author:
N.K. Van Engen; R. Platt; J.A. Roth; M.L. Stock; T. Engelken; R.C. Vann; L.W. Wulf; W.D. Busby; C. Wang; E.M. Kalkwarf; J.F. Coetzee
Source:
Veterinary immunology and immunopathology 2016 v.175 pp. 42-50
ISSN:
0165-2427
Subject:
interferon-gamma; placebos; vaccination; transportation; antibodies; cell-mediated immunity; steers; CD8-positive T-lymphocytes; lactose; blood serum; antigens; humoral immunity; body weight; feedlots; meloxicam; Brahman; analysis of variance; flow cytometry; secondary immunization; Bovine viral diarrhea virus 1; crossbreds; cortisol; interleukin-4; Iowa; Mississippi
Abstract:
... The objective of this study was to investigate the impact of oral meloxicam (MEL) and long-distance transportation on cell-mediated immunity (CMI) in preconditioned steers receiving a booster vaccination on arrival. We hypothesized that steers treated with MEL at 1mg/kg body weight, 6h before night-time transport, would be less immunocompromised on arrival (day 0) and after 7days, and that CMI fol ...
DOI:
10.1016/j.vetimm.2016.05.006
CHORUS:
10.1016/j.vetimm.2016.05.006
Chorus Open Access:
10.1016/j.vetimm.2016.05.006

51. In vitro assessment of the effects of temperature on phagocytosis, reactive oxygen species production and apoptosis in bovine polymorphonuclear cells

Author:
Cristina Lecchi; Nicola Rota; Andrea Vitali; Fabrizio Ceciliani; Nicola Lacetera
Source:
Veterinary immunology and immunopathology 2016 v.182 pp. 89-94
ISSN:
0165-2427
Subject:
Escherichia coli; Holstein; adverse effects; animal health; apoptosis; caspase-3; caspase-7; cytochrome c; dairy cows; equations; fever; fluorescence; heat stress; immunosuppression; in vitro studies; lactation; leukocytes; mastitis; phagocytosis; physiological response; reactive oxygen species; risk; somatic cell count; t-test; weather
Abstract:
... Heat stress exerts a direct negative effect on farm animal health, triggering physiological responses. Environmental high temperature induces immunosuppression in dairy cows, increasing the risk of mastitis and milk somatic cell counts. The influence of heat stress on leukocytes activities has not been fully elucidated. The present in vitro study was aimed at assessing whether the exposure to temp ...
DOI:
10.1016/j.vetimm.2016.10.007

52. In vitro cow uterine response to Escherichia coli, leukotrienes and cytokines

Author:
A.J. Korzekwa; M. Łupicka; B.M- Socha; A.A. Szczepańska; E. Piotrowicz; W. Barański
Source:
Veterinary immunology and immunopathology 2016 v.182 pp. 59-62
ISSN:
0165-2427
Subject:
Escherichia coli; Toll-like receptor 4; antagonists; explants; gene expression; interferon-gamma; interleukin-6; leukotrienes; messenger RNA; prostaglandins; secretion; tumor necrosis factors; uterus
Abstract:
... The aim was to determine the dynamic profile of interactions between Escherichia coli (E. coli) and the actions of leukotrienes (LTs) and TNF and INFγ (cytokines) in the uterus in vitro. Uterine explants (N=6) were incubated for 2, 12 and 24h either as E. coli-treated (106CFU) or non-treated and/or with: LTB4 and C4 (10−6M, for both LTs), LTs receptors antagonists (aLTR; 10−6M) and/or cytokines (e ...
DOI:
10.1016/j.vetimm.2016.10.001

53. In vivo relevance of polymorphic Interleukin 8 promoter haplotype for the systemic immune response to LPS in Holstein-Friesian calves

Author:
Bojan Stojkovic; Rachel M. McLoughlin; Kieran G. Meade
Source:
Veterinary immunology and immunopathology 2016 v.182 pp. 1-10
ISSN:
0165-2427
Subject:
Holstein; Jersey; calves; endotoxins; epithelial cells; farms; gene expression; genes; genetic techniques and protocols; genetic variation; haplotypes; hematologic tests; immune response; inflammation; interleukin-8; loci; mammary glands; neutrophils; promoter regions; protein synthesis
Abstract:
... Interleukin 8, also known as CXC chemokine ligand 8 (CXCL8), is a critical chemokine in the recruitment of leukocytes to sites of infection and is a potent mediator of inflammation. We previously discovered 29 polymorphic sites in the promoter region of the bovine Interleukin 8 gene, which segregate into two distinct haplotypes, denoted IL8-h1 and IL8-h2. Population genetic analysis of these two h ...
DOI:
10.1016/j.vetimm.2016.09.006

54. Influence of energy level and nicotinic acid supplementation on apoptosis of blood leukocytes of periparturient dairy cows

Author:
S. Bühler; J. Frahm; R. Tienken; S. Kersten; U. Meyer; K. Huber; S. Dänicke
Source:
Veterinary immunology and immunopathology 2016 v.179 pp. 36-45
ISSN:
0165-2427
Subject:
3-hydroxybutyric acid; Holstein; anti-inflammatory activity; apoptosis; caspases; correlation; dairy cows; energy; flow cytometry; fluorescence; free fatty acids; genes; immunosuppression; lipolysis; messenger RNA; metabolic diseases; mononuclear leukocytes; neutrophils; niacin; parturition; propidium; quantitative polymerase chain reaction; transcription factor NF-kappa B
Abstract:
... The periparturient period of dairy cows is accompanied by an immunosuppression that leaves the animal more susceptible to infections and metabolic disorders. Non-esterified fatty acids (NEFA) and beta-hydroxybutyrate (BHB) which peak shortly after parturition due to lipolysis are known to impair immune cell functions. Niacin with its well-known anti-lipolytic effect may have the ability to amelior ...
DOI:
10.1016/j.vetimm.2016.07.007

55. Innate immune responses of young bulls to a novel environment

Author:
Elisabetta Razzuoli; Emilio Olzi; Pietro Calà; Simona Cafazzo; Diego Magnani; Andrea Vitali; Nicola Lacetera; Laura Archetti; Fabrizio Lazzara; Angelo Ferrari; Leonardo Nanni Costa; Massimo Amadori
Source:
Veterinary immunology and immunopathology 2016 v.172 pp. 9-13
ISSN:
0165-2427
Subject:
Holstein; animal welfare; antibacterial properties; beta-globulins; blood serum; breeding; bulls; climate; complement; dairy farming; farming systems; gamma-globulins; innate immunity; interleukin-6; physical models; psychological stress; serum albumin; transportation; tumor necrosis factor-alpha
Abstract:
... Animal welfare during transportation has been investigated in several studies, as opposed to post-transportation phases. In this study, we evaluated the effect of a novel environment after transportation on 26 Friesian bulls, 242±42 day-old, from ten different dairy farms. Animals were shipped to a breeding center in different seasons, and selected parameters of innate immunity (serum bactericidal ...
DOI:
10.1016/j.vetimm.2016.02.014

56. Leptospira spp.: Novel insights into host–pathogen interactions

Author:
Luis G. Fernandes; Gabriela H. Siqueira; Aline R.F. Teixeira; Lucas P. Silva; Jupciana M. Figueredo; Maria R. Cosate; Monica L. Vieira; Ana L.T.O. Nascimento
Source:
Veterinary immunology and immunopathology 2016 v.176 pp. 50-57
ISSN:
0165-2427
Subject:
Leptospira; adhesins; antigens; bacteria; bacterial adhesion; bacterial colonization; binding proteins; coagulation; complement; death; diagnostic techniques; extracellular matrix; fibrinogen; financial economics; hemorrhage; host-pathogen relationships; humans; immune system; leptospirosis; livestock; milk production; models; nucleotide sequences; pathogenesis; plasmin; plasminogen; proteolysis; receptors; tissues; vaccines; zoonoses
Abstract:
... Leptospirosis is a widespread zoonosis caused by pathogenic Leptospira spp. It is an important infectious disease that affects humans and animals. The disease causes economic losses as it affects livestock, with decreased milk production and death. Our group is investigating the genome sequences of L. interrogans targeting surface-exposed proteins because, due to their location, these proteins are ...
DOI:
10.1016/j.vetimm.2015.12.004

57. Longitudinal evaluation of leukocyte transcripts in killer whales (Orcinus Orca)

Author:
Tatjana Sitt; Lizabeth Bowen; Chia-Shan Lee; Myra T. Blanchard; James McBain; Christopher Dold; Jeffrey L. Stott
Source:
Veterinary immunology and immunopathology 2016 v.175 pp. 7-15
ISSN:
0165-2427
Subject:
Orcinus orca; biomarkers; blood sampling; decision making; diagnostic techniques; disease diagnosis; genes; health care workers; interleukin-10; interleukin-17; interleukin-4; leukocytes; messenger RNA; whales
Abstract:
... Early identification of illness and/or presence of environmental and/or social stressors in free-ranging and domestic cetaceans is a priority for marine mammal health care professionals. Incorporation of leukocyte gene transcript analysis into the diagnostic tool kit has the potential to augment classical diagnostics based upon ease of sample storage and shipment, inducible nature and well-defined ...
DOI:
10.1016/j.vetimm.2016.04.011

58. Marek’s disease virus immunosuppression alters host cellular responses and immune gene expression in the skin of infected chickens

Author:
Mohammad Heidari; Dan Wang; Phillip Delekta; Shuhong Sun
Source:
Veterinary immunology and immunopathology 2016 v.180 pp. 21-28
ISSN:
0165-2427
Subject:
CD4-positive T-lymphocytes; Mardivirus; Marek disease; animal organs; cell adhesion; cell-mediated immunity; chickens; cytotoxic T-lymphocytes; epithelium; gene expression; gene expression regulation; genes; glycoproteins; immune evasion; immune response; immunosuppression; peripheral nerves; virion; virus replication; viruses
Abstract:
... Marek’s disease virus (MDV), a highly cell-associated lymphotropic α-herpesvirus, is the causative agent of Marek’s disease (MD) in domestic chickens. MDV replicates in chicken lymphocytes and establishes a latent infection within CD4+ T cells. The latently infected CD4+ T cells carry the virus to visceral organs, peripheral nerves, and feather follicle epithelium (FFE). FFE is the only anatomical ...
DOI:
10.1016/j.vetimm.2016.08.013

59. Multinucleated giant cell cytokine expression in pulmonary granulomas of cattle experimentally infected with Mycobacterium bovis

Author:
Mitchell V. Palmer; Tyler C. Thacker; W. Ray Waters
Source:
Veterinary immunology and immunopathology 2016 v.180 pp. 34-39
ISSN:
0165-2427
Subject:
Mycobacterium bovis; bovine tuberculosis; calves; chemokines; enzymes; foreign bodies; gene expression regulation; giant cells; granuloma; humans; interferon-gamma; interleukin-10; interleukin-17; lymphocytes; macrophages; pathogens; transforming growth factor beta; tumor necrosis factor-alpha
Abstract:
... Regardless of host, pathogenic mycobacteria of the Mycobacterium tuberculosis complex such as Mycobacterium bovis, induce a characteristic lesion known as a granuloma, tubercle or tuberculoid granuloma. Granulomas represent a distinct host response to chronic antigenic stimuli, such as foreign bodies, certain bacterial components, or persistent pathogens such as M. bovis. Granulomas are composed o ...
DOI:
10.1016/j.vetimm.2016.08.015
CHORUS:
10.1016/j.vetimm.2016.08.015
Chorus Open Access:
10.1016/j.vetimm.2016.08.015

60. NCR1 is an activating receptor expressed on a subset of canine NK cells

Author:
Christine Grøndahl-Rosado; Preben Boysen; Grethe M. Johansen; Hege Brun-Hansen; Anne K. Storset
Source:
Veterinary immunology and immunopathology 2016 v.177 pp. 7-15
ISSN:
0165-2427
Subject:
cell culture; cultured cells; dogs; interleukin-12; interleukin-15; interleukin-2; natural killer cells; vaccination
Abstract:
... Defining NK cells has been challenging in many veterinary species. Although several groups have described putative NK cell populations, there is still no consensus on a definition of NK cells in the dog. In the present study, canine NK cells are characterized as CD3−GranzymeB+ cells, further divided into a NCR1+ and a NCR1− subset. All dogs examined displayed both subsets in blood, although of qui ...
DOI:
10.1016/j.vetimm.2016.05.001

61. NCR1+ cells appear early in GALT development of the ovine foetus and acquire a c-kit+ phenotype towards the end of gestation

Author:
Line Olsen; Caroline Piercey Åkesson; Mona Aleksandersen; Preben Boysen; Charles McL. Press; Françoise Drouet; Anne K. Storset; Arild Espenes
Source:
Veterinary immunology and immunopathology 2016 v.169 pp. 79-84
ISSN:
0165-2427
Subject:
T-lymphocytes; antibodies; developmental stages; digestive system; fetus; phenotype; pregnancy; sheep
Abstract:
... The amount, distribution and phenotype of ovine NCR1+ cells were investigated during developing GALT from day 70 of gestation. Antibodies against CD3 and CD79 were used to identify the compartments of GALT, and the localization of NCR1+ cells were correlated within these structures. Markers CD34 and c-kit, in addition to Ki67, were used to investigate possible origin and the stage of development o ...
DOI:
10.1016/j.vetimm.2015.12.008

62. Nasal delivery of chitosan-coated poly(lactide-co-glycolide)-encapsulated honeybee (Apis mellifera) venom promotes Th 1-specific systemic and local intestinal immune responses in weaned pigs

Author:
Jin-A Lee; Yun-Mi Kim; Tae-Hoon Kim; Sang-Ho Lee; Cho-A Lee; Cheong-Weon Cho; Jong-woon Jeon; Jin-kyu Park; Sang-Ki Kim; Bock-Gie Jung; Bong-Joo Lee
Source:
Veterinary immunology and immunopathology 2016 v.178 pp. 99-106
ISSN:
0165-2427
Subject:
Apis mellifera; drugs; honey bees; ileum; immune response; interferon-gamma; interleukin-12; interleukin-2; intranasal administration; lymphocyte proliferation; mononuclear leukocytes; nose; spleen; swine; transcription factors; venoms
Abstract:
... Nasal delivery is a convenient and acceptable route for drug administration, and has been shown to elicit a much more potent local and systemic response compared with other drug delivery routes. We previously demonstrated that rectal administration of poly(lactide-co-glycolide)-encapsulated honeybee venom (P-HBV) could enhance systemic Th 1-specific immune responses. We therefore synthesized chito ...
DOI:
10.1016/j.vetimm.2016.07.005

63. Natural autoantibodies in Bos taurus calves during the first twelve weeks of life

Author:
N. Mayasari; A.T.M. Van Knegsel; G. de Vries Reilingh; B. Kemp; H.K. Parmentier
Source:
Veterinary immunology and immunopathology 2016 v.178 pp. 70-78
ISSN:
0165-2427
Subject:
autoantibodies; calves; calving; carbonate dehydratase; colostrum; cows; drinking; glutamate dehydrogenase; homeostasis; immunoglobulin G; immunoglobulin M; mothers; myosin; transferrin
Abstract:
... Natural autoantibodies (NAAb) have a role in maintaining physiological homeostasis and prevention of infections, and have been found in mammalian species tested so far. Albeit NAAb levels rise with age, little is known about the origin, function, regulation and initiation of NAAb in young animals. The present study addressed the presence of IgM and IgG NAAb binding glutamate dehydrogenase (GD), ca ...
DOI:
10.1016/j.vetimm.2016.07.001

64. Optimisation and validation of a PCR for antigen receptor rearrangement (PARR) assay to detect clonality in canine lymphoid malignancies

Author:
Elspeth M. Waugh; Alice Gallagher; Hayley Haining; Pamela E.J. Johnston; Francesco Marchesi; Ruth F. Jarrett; Joanna S. Morris
Source:
Veterinary immunology and immunopathology 2016 v.182 pp. 115-124
ISSN:
0165-2427
Subject:
DNA primers; antigens; cell biology; diagnostic techniques; dogs; flow cytometry; genes; immunohistochemistry; lymphoma; patients; polymerase chain reaction; receptors
Abstract:
... PCR for antigen receptor gene rearrangements (PARR) analysis is being increasingly used to assist diagnosis of canine lymphoma. In this study, PARR was carried out on consecutive samples received as part of routine diagnostic practice from 271 patients: 195 with lymphoid malignancies, 53 with reactive conditions and 23 with other neoplasms. Initially, published primer sets were used but later mino ...
DOI:
10.1016/j.vetimm.2016.10.008
PubMed:
27863542
PubMed Central:
PMC5119497

65. Oral vaccination of cattle with heat inactivated Mycobacterium bovis does not compromise bovine TB diagnostic tests

Author:
Gareth J. Jones; Sabine Steinbach; Iker A. Sevilla; Joseba M. Garrido; Ramon Juste; H. Martin Vordermeier
Source:
Veterinary immunology and immunopathology 2016 v.182 pp. 85-88
ISSN:
0165-2427
Subject:
Mycobacterium bovis; calves; diagnostic techniques; heat inactivation; immune response; interferon-gamma; oral vaccination; tuberculin; vaccines; wildlife
Abstract:
... In this study we investigated whether oral uptake of a heat inactivated M. bovis wildlife vaccine by domestic cattle induced systemic immune responses that compromised the use of tuberculin or defined antigens in diagnostic tests for bovine TB. Positive skin test and blood-based IFN-γ release assay (IGRA) results were observed in all calves vaccinated via the parenteral route (i.e. intramuscular). ...
DOI:
10.1016/j.vetimm.2016.10.010

66. Oxidative stress in hoof laminar tissue of horses with lethal gastrointestinal diseases

Author:
Luciane Maria Laskoski; Rosangela Locatelli Dittrich; Carlos Augusto Araújo Valadão; Juliana Sperotto Brum; Yara Brandão; Harald Fernando Vicente Brito; Renato Silva de Sousa
Source:
Veterinary immunology and immunopathology 2016 v.171 pp. 66-72
ISSN:
0165-2427
Subject:
digestive system diseases; gastrointestinal system; hooves; horses; laminitis; oxidative stress; pathogenesis; superoxide dismutase
Abstract:
... Tissue damage caused by oxidative stress is involved in the pathogenesis of several diseases in animals and man, and is believed to play a role in the development of laminitis in horses. The aim of this study was to investigate the oxidative stress associated with laminar lesions in horses with lethal gastrointestinal disorders. Laminar tissue samples of the hoof of 30 horses were used. Tissue sam ...
DOI:
10.1016/j.vetimm.2016.02.008

67. Passive immunisation, an old idea revisited: Basic principles and application to modern animal production systems

Author:
Chris J. Hedegaard; Peter M.H. Heegaard
Source:
Veterinary immunology and immunopathology 2016 v.174 pp. 50-63
ISSN:
0165-2427
Subject:
animal production; antibiotics; antibodies; cattle; feed additives; fish; goats; immunoglobulins; immunologic memory; infectious diseases; pathogens; poultry; production technology; risk; sheep; spray drying; swine; toxins; vaccination
Abstract:
... Immunisation by administration of antibodies (immunoglobulins) has been known for more than one hundred years as a very efficient means of obtaining immediate, short-lived protection against infection and/or against the disease-causing effects of toxins from microbial pathogens and from other sources. Thus, due to its rapid action, passive immunisation is often used to treat disease caused by infe ...
DOI:
10.1016/j.vetimm.2016.04.007
PubMed:
27185263
PubMed Central:
PMC7127230

68. Polymorphisms in ERAP1 and ERAP2 are shared by Caninae and segregate within and between random- and pure-breeds of dogs

Author:
N.C. Pedersen; J.K. Dhanota; H. Liu
Source:
Veterinary immunology and immunopathology 2016 v.179 pp. 46-57
ISSN:
0165-2427
Subject:
Canis latrans; Vulpes vulpes; amino acids; autoimmune diseases; dog breeds; dogs; endoplasmic reticulum; genes; haplotypes; humans; infectious diseases; jackals; neoplasms; peptides; phylogeny; purebreds; receptors; risk; single nucleotide polymorphism; wolves
Abstract:
... Specific polymorphisms in the endoplasmic reticulum amino peptidase genes ERAP1 and ERAP2, when present with certain MHC class receptor types, have been associated with increased risk for specific cancers, infectious diseases and autoimmune disorders in humans. This increased risk has been linked to distinct polymorphisms in both ERAPs and MHC class I receptors that affect the way cell-generated p ...
DOI:
10.1016/j.vetimm.2016.08.006

69. Production a monoclonal antibody specific to granulocytes of swimming crab (Portunus trituberculatus) and its cross reactivity with other crustaceans

Author:
Shun-feng Cheng; Xiao-chun Wu; Min Zhang
Source:
Veterinary immunology and immunopathology 2016 v.178 pp. 79-87
ISSN:
0165-2427
Subject:
Eriocheir sinensis; Portunus trituberculatus; antigens; crabs; cross reaction; flow cytometry; granulocytes; hemocytes; mice; molecular weight; monoclonal antibodies
Abstract:
... In this study, a monoclonal antibody (mAb) 3F4 specific to granulocytes of swimming crab, Portunus trituberculatus, was obtained by immunizing mice with whole haemocytes. mAb 3F4 showed strong immunofluorescent reaction with granulocytes, but no reaction with hyalinocytes. The positive cell percentage of granulocytes was 86.3% detected by Flow cytometry (FCM). A special antigen with molecular weig ...
DOI:
10.1016/j.vetimm.2016.07.006

70. Protective efficacy of Streptococcus iniae derived enolase against Streptococcal infection in a zebrafish model

Author:
Juver D. Membrebe; Nam-Kyung Yoon; Minhee Hong; Jeongsoo Lee; Hongweon Lee; Kyoungmoon Park; Sin-hye Seo; Injoong Yoon; Sungsik Yoo; Yeu-Chun Kim; Jungoh Ahn
Source:
Veterinary immunology and immunopathology 2016 v.170 pp. 25-29
ISSN:
0165-2427
Subject:
Danio rerio; Escherichia coli; Streptococcus iniae; Streptococcus parauberis; antiserum; cross immunity; fish; models; pathogens; phosphopyruvate hydratase; proteins; serotypes; vaccination; vaccines
Abstract:
... Enolase (ENO) is one of the surface-exposed proteins of Streptococcus iniae, which previously had been identified as a plasminogen-binding protein. In this study, ENO was evaluated to induce cross-protective immunity against S. iniae and Streptococcus parauberis which are major pathogens causing streptococcosis in fish. Immunoblot analysis shows that S. iniae recombinant ENO (rENO) produced in Esc ...
DOI:
10.1016/j.vetimm.2016.01.004

71. Recombinant Mycoplasma mycoides proteins elicit protective immune responses against contagious bovine pleuropneumonia

Author:
Isabel Nkando; Jose Perez-Casal; Martin Mwirigi; Tracy Prysliak; Hugh Townsend; Emil Berberov; Joseph Kuria; John Mugambi; Reuben Soi; Anne Liljander; Joerg Jores; Volker Gerdts; Andrew Potter; Jan Naessens; Hezron Wesonga
Source:
Veterinary immunology and immunopathology 2016 v.171 pp. 103-114
ISSN:
0165-2427
Subject:
Boran; Mycoplasma mycoides subsp. mycoides; adverse effects; antibodies; antigens; cattle; contagious bovine pleuropneumonia; immune response; immunization; males; recombinant proteins; temperature; vaccine development; vaccines; zebu; Sub-Saharan Africa
Abstract:
... Mycoplasma mycoides subsp. mycoides (Mmm) is the causative agent of contagious bovine pleuropneumonia (CBPP), a devastating respiratory disease mainly affecting cattle in sub-Saharan Africa. The current vaccines are based on live-attenuated Mmm strains and present problems with temperature stability, duration of immunity and adverse reactions, thus new vaccines are needed to overcome these issues. ...
DOI:
10.1016/j.vetimm.2016.02.010

72. Reduced humoral immunity and atypical cell-mediated immunity in response to vaccination in cows naturally infected with bovine leukemia virus

Author:
Meredith C. Frie; Kelly R. Sporer; Joseph C. Wallace; Roger K. Maes; Lorraine M. Sordillo; Paul C. Bartlett; Paul M. Coussens
Source:
Veterinary immunology and immunopathology 2016 v.182 pp. 125-135
ISSN:
0165-2427
Subject:
dairy cows; Bovine alphaherpesvirus 1; immunoglobulin G; immunoglobulin M; Leptospira interrogans serovar Hardjo; humoral immunity; Bovine leukemia virus; cell-mediated immunity; flow cytometry; lactation; vaccination; adults; Bovine viral diarrhea virus 1; Holstein; immune response; neutralizing antibodies; CD8-positive T-lymphocytes; enzootic bovine leukosis
Abstract:
... Bovine leukemia virus (BLV) is a retrovirus that is widely distributed across US dairy herds: over 83% of herds are BLV-infected and within-herd infection rates can approach 50%. BLV infection reduces both animal longevity and milk production and can interfere with normal immune health. With such a high prevalence of BLV infection in dairy herds, it is essential to understand the circumstances by ...
DOI:
10.1016/j.vetimm.2016.10.013

73. Regulatory T cells and immune profiling in johne’s disease lesions

Author:
Jonathan A. Roussey; Lilian J. Oliveira; Ingeborg M. Langohr; Dodd G. Sledge; Paul M. Coussens
Source:
Veterinary immunology and immunopathology 2016 v.181 pp. 39-50
ISSN:
0165-2427
Subject:
Mycobacterium avium subsp. paratuberculosis; T-lymphocytes; animal health; chronic diseases; chronic wasting disease; death; diarrhea; gene expression; genes; homeostasis; ileum; inflammation; lymph nodes; macrophages; microbial load; mycobacterial diseases; ruminants; tissues; weight loss
Abstract:
... Johne’s disease, caused by infection with Mycobacterium avium subspecies paratuberculosis (MAP), is a chronic wasting disease of ruminants. Hallmark symptoms of clinical Johne’s disease include diarrhea, progressive weight loss, and premature death; symptoms due largely to chronic inflammation in the small intestine. MAP colonizes resident macrophages within the ileum of the small intestine, subse ...
DOI:
10.1016/j.vetimm.2016.03.008
CHORUS:
10.1016/j.vetimm.2016.03.008
Chorus Open Access:
10.1016/j.vetimm.2016.03.008

74. Response of immune response genes to adjuvants poly [di(sodium carboxylatoethylphenoxy)phosphazene] (PCEP), CpG oligodeoxynucleotide and emulsigen at intradermal injection site in pigs

Author:
R.B. Magiri; K. Lai; A.M. Chaffey; H.L. Wilson; W.E. Berry; M.L. Szafron; G.K. Mutwiri
Source:
Veterinary immunology and immunopathology 2016 v.175 pp. 57-63
ISSN:
0165-2427
Subject:
Toll-like receptor 9; adaptive immunity; adjuvants; chemokine CCL2; chemokine CCL5; gene expression regulation; genes; immune response; injection site; innate immunity; interferons; interleukin-10; interleukin-17; interleukin-6; oligodeoxyribonucleotides; sodium; swine; tumor necrosis factor-alpha; vaccines
Abstract:
... Understanding the mechanisms by which adjuvants mediate their effects provide critical information on how innate immunity influences the development of adaptive immunity. Despite being a critical vaccine component, the mechanisms by which adjuvants mediate their effects are not fully understood and this is especially true when they are used in large animals. This lack of understanding limits our a ...
DOI:
10.1016/j.vetimm.2016.05.003

75. SOLiD SAGE sequencing shows differential gene expression in jejunal lymph node samples of resistant and susceptible red deer (Cervus elaphus) challenged with Mycobacterium avium subsp. paratuberculosis

Author:
C.G. Mackintosh; J.F.T. Griffin; I.C. Scott; R. O’Brien; J.L. Stanton; P. MacLean; R. Brauning
Source:
Veterinary immunology and immunopathology 2016 v.169 pp. 102-110
ISSN:
0165-2427
Subject:
Cervus elaphus; Mycobacterium avium subsp. paratuberculosis; antigen presentation; apoptosis; deer; gene expression; gene expression regulation; genes; high-throughput nucleotide sequencing; histopathology; inflammation; interferons; interleukins; jejunum; lymph nodes; microbial load; mitochondria; mucosal immunity; paratuberculosis; phenotype
Abstract:
... This study compared in vivo lymph node gene expression levels between six young red deer that were either relatively resistant (R) or susceptible (S) to paratuberculosis following experimental challenge with Mycobacterium avium subsp. paratuberculosis. Intestinal lymph nodes were biopsied at 4, 12 and 50 weeks post challenge (pc) and parallel changes in histopathology, immunology and bacterial loa ...
DOI:
10.1016/j.vetimm.2015.10.009

76. Single nucleotide polymorphisms in major histocompatibility class II haplotypes are associated with potential resistance to inflammatory bowel disease in German shepherd dogs

Author:
Atiyeh Peiravan; Karin Allenspach; Alisdair M. Boag; Francesca Soutter; Angela Holder; Brian Catchpole; Lorna J. Kennedy; Dirk Werling; Fabio Procoli
Source:
Veterinary immunology and immunopathology 2016 v.182 pp. 101-105
ISSN:
0165-2427
Subject:
German Shepherd; antigens; confidence interval; dogs; etiology; gastrointestinal system; genotyping; haplotypes; inflammatory bowel disease; leukocytes; loci; major histocompatibility complex; odds ratio; risk; risk reduction; single nucleotide polymorphism; United Kingdom
Abstract:
... German shepherd dogs (GSD) in the UK are at increased risk of developing the Inflammatory Bowel Disaese (IBD). IBD is believed to be a multifactorial immune mediated disease affecting genetically predisposed dogs. The aim of the current study was to investigate whether susceptibility to IBD in GSD is associated with the major histocompatibility complex (MHC) class II locus (Dog Leukocyte Antigen, ...
DOI:
10.1016/j.vetimm.2016.10.012

77. Tissue-specific mRNA expression profiles of porcine Toll-like receptors at different ages in germ-free and conventional pigs

Author:
Lulu Shao; David D. Fischer; Sukumar Kandasamy; Linda J. Saif; Anastasia N. Vlasova
Source:
Veterinary immunology and immunopathology 2016 v.171 pp. 7-16
ISSN:
0165-2427
Subject:
Toll-like receptors; adaptive immunity; antigens; autoimmune diseases; dendritic cells; gene expression; ileum; infectious diseases; innate immunity; messenger RNA; microorganisms; neonates; swine
Abstract:
... Toll-like receptors (TLRs), key initiators of innate immune responses, recognize antigens and are essential in linking innate and adaptive immune responses. Misrecognition and over-stimulation/expression of TLRs may contribute to the development of chronic inflammatory diseases and autoimmune diseases. However, appropriate and mature TLR responses are associated with the establishment of resistanc ...
DOI:
10.1016/j.vetimm.2016.01.008
PubMed:
26964712
PubMed Central:
PMC4788813

78. Tracing the evolutionary lineage of pattern recognition receptor homologues in vertebrates: An insight into reptilian immunity via de novo sequencing of the wall lizard splenic transcriptome

Author:
Manisha Priyam; Mamta Tripathy; Umesh Rai; Soma Mondal Ghorai
Source:
Veterinary immunology and immunopathology 2016 v.172 pp. 26-37
ISSN:
0165-2427
Subject:
Hemidactylus; Toll-like receptor 3; Toll-like receptor 4; Toll-like receptor 5; Toll-like receptor 7; antigens; databases; defense mechanisms; genes; harvesting; immune system; immunity; oligomerization; pathogens; phylogeny; signal transduction; transcriptome; wall lizards
Abstract:
... Reptiles remain a deprived class in the area of genomic and molecular resources for the vertebrate classes. The transition of squamates from aquatic to terrestrial mode of life caused profound changes in their immune system to combat the altered variety of pathogens on land. The current study aims at delineating the evolution of defence mechanisms in wall lizard, Hemidactylus flaviviridis, by expl ...
DOI:
10.1016/j.vetimm.2016.03.002

79. Transcriptome analysis of the endangered Chinese giant salamander (Andrias davidianus): Immune modulation in response to Aeromonas hydrophila infection

Author:
Zhitao Qi; Qihuan Zhang; Zisheng Wang; Tianyi Ma; Jie Zhou; Jason W. Holland; Qian Gao
Source:
Veterinary immunology and immunopathology 2016 v.169 pp. 85-95
ISSN:
0165-2427
Subject:
Aeromonas hydrophila; Andrias; bacterial infections; breeding; control methods; disease control; disease outbreaks; evolution; gene expression regulation; habitats; immune system; immunity; pathogens; salamanders and newts; transcriptomics; unigenes; viability
Abstract:
... The endangered Chinese giant salamander (Andrias davidianus) is the largest extant amphibian species. Disease outbreaks represent one of the major factors threatening A. davidianus populations in the wild and the viability of artificial breeding programmes. Development of future immune therapies to eliminate infectious disease in A. davidianus is dependent on a thorough understanding of the immune ...
DOI:
10.1016/j.vetimm.2015.11.004

80. Two routes of infection with Photobacterium damselae subsp. piscicida are effective in the modulation of the transcription of immune related genes in Solea senegalensis

Author:
J.A. Núñez-Díaz; M. Fumanal; J.M. Mancera; M.A. Moriñigo; M.C. Balebona
Source:
Veterinary immunology and immunopathology 2016 v.179 pp. 8-17
ISSN:
0165-2427
Subject:
Photobacterium damselae subsp. piscicida; Solea senegalensis; antibodies; complement; disease outbreaks; farmed fish; gene induction; genes; heat shock proteins; innate immunity; intraperitoneal injection; iron; iron absorption; kidneys; liver; lysozyme; marine fish; pathogens; tissues; transcription (genetics); transferrin; tumor necrosis factor-alpha
Abstract:
... The marine fish pathogen Photobacterium damselae subsp. piscicida (Phdp) is responsible for important disease outbreaks affecting cultured fish species including the flatfish Solea senegalensis. In the present work, transcription of iron metabolism related genes (TF, FERR-M, HP-1 and HAMP-1) as well as innate immune system components such as complement proteins (C3 and C7), lysozyme (LYS-G), TNF f ...
DOI:
10.1016/j.vetimm.2016.07.009

81. A chimeric protein comprising the immunogenic domains of Mannheimia haemolytica leukotoxin and outer membrane protein PlpE induces antibodies against leukotoxin and PlpE

Author:
Sai Arun Batra; Sudarvili Shanthalingam; Gaetano Donofrio; Subramaniam Srikumaran
Source:
Veterinary immunology and immunopathology 2016 v.175 pp. 36-41
ISSN:
0165-2427
Subject:
Mannheimia haemolytica; Ovis canadensis; antibodies; cytotoxicity; epitopes; habitats; immune response; leukotoxins; mice; mortality; outer membrane proteins; pathogens; pneumonia; recombinant fusion proteins; ruminants; surface antigens; vaccination; vaccine development; vaccines; virulence; North America
Abstract:
... Mannheimia haemolytica is a very important pathogen of pneumonia in ruminants. Bighorn sheep (BHS, Ovis canadensis) are highly susceptible to M. haemolytica-caused pneumonia which has significantly contributed to the drastic decline of bighorn sheep population in North America. Pneumonia outbreaks in wild BHS can cause mortality as high as 90%. Leukotoxin is the critical virulence factor of M. hae ...
DOI:
10.1016/j.vetimm.2016.05.004

82. A comparison of serum and plasma cytokine values using a multiplexed assay in cats

Author:
Margaret E. Gruen; Kristen M. Messenger; Andrea E. Thomson; Emily H. Griffith; Hayley Paradise; Shelly Vaden; B.D.X. Lascelles
Source:
Veterinary immunology and immunopathology 2016 v.182 pp. 69-73
ISSN:
0165-2427
Subject:
EDTA (chelating agent); anticoagulants; bioassays; blood serum; cats; chemokines; morbidity; osteoarthritis; pain
Abstract:
... Degenerative joint disease (DJD) is highly prevalent in cats, and pain contributes to morbidity. In humans, alterations of cytokine concentrations have been associated with joint deterioration and pain. Similar changes have not been investigated in cats. Cytokine concentrations can be measured using multiplex technology with small samples of serum or plasma, however, serum and plasma are not inter ...
DOI:
10.1016/j.vetimm.2016.10.003
PubMed:
27863553
PubMed Central:
PMC5522725

83. The function of feline stimulator of interferon gene (STING) is evolutionarily conserved

Author:
Xiaozhan Zhang; Hongxia Wu; Chunguo Liu; Xue Sun; Shaopo Zu; Jin Tian; Liandong Qu; Shoujun Li
Source:
Veterinary immunology and immunopathology 2016 v.169 pp. 54-62
ISSN:
0165-2427
Subject:
amino acids; cats; cattle; endoplasmic reticulum; genes; interferon-beta; lungs; lymph nodes; spleen; transcription factor NF-kappa B
Abstract:
... Stimulator of interferon gene (STING) mediates the induction of type I IFN responses. In this study, feline STING was cloned. Full-length STING contains 1134bp and encodes a 377 amino acid product that shares the highest similarity with bovine STING. STING is primarily expressed in the spleen, lungs and lymph nodes. An examination of its cellular localization indicated that STING is localized in t ...
DOI:
10.1016/j.vetimm.2015.12.005

84. The stability of plasma IP-10 enhances its utility for the diagnosis of Mycobacterium bovis infection in African buffaloes (Syncerus caffer)

Author:
Wynand J. Goosen; Paul D. van Helden; Robin M. Warren; Michele A. Miller; Sven D.C. Parsons
Source:
Veterinary immunology and immunopathology 2016 v.173 pp. 17-20
ISSN:
0165-2427
Subject:
Mycobacterium bovis; Syncerus caffer; biomarkers; blood; buffaloes; heat inactivation; heat treatment; humans; interferons; peptides; phytohemagglutinin; storage conditions; temperature
Abstract:
... The measurement of interferon gamma-induced protein 10 (IP-10) in antigen-stimulated whole blood is a sensitive biomarker of Mycobacterium bovis infection in African buffaloes (Syncerus caffer). However, this species often occurs in remote locations and diagnostic samples must be transported to centralised laboratories for processing. In humans, plasma IP-10 is highly stable and this feature contr ...
DOI:
10.1016/j.vetimm.2016.03.013

85. A vaccine based on a mutant transferrin binding protein B of Haemophilus parasuis induces a strong T-helper 2 response and bacterial clearance after experimental infection

Author:
Sonia Martínez-Martínez; Rafael Frandoloso; Elías-Fernando Rodríguez-Ferri; María-José García-Iglesias; Claudia Pérez-Martínez; Álvaro Álvarez-Estrada; César-Bernardo Gutiérrez-Martín
Source:
Veterinary immunology and immunopathology 2016 v.179 pp. 18-25
ISSN:
0165-2427
Subject:
Haemophilus parasuis; antibodies; binding proteins; enzyme-linked immunosorbent assay; immune response; interleukin-4; interleukin-5; interleukin-8; macrophages; mutants; secretion; spleen; swine; temperature; transferrin; vaccination; vaccines
Abstract:
... This study aimed to characterize the type of immune response induced by an experimental vaccine based on a mutant Haemophilus parasuis transferrin binding protein (Tbp) B (Y167A) defective in its ability to bind porcine transferrin. Clinical and pathological signs, bacterial clearance, antibody response and the cytokine profile in alveolar macrophages and spleen after the vaccination and challenge ...
DOI:
10.1016/j.vetimm.2016.07.011

86. γδ T cells and the immune response to respiratory syncytial virus infection

Author:
Jodi L. McGill; Randy E. Sacco
Source:
Veterinary immunology and immunopathology 2016 v.181 pp. 24-29
ISSN:
0165-2427
Subject:
immunopathology; humans; T-lymphocytes; morbidity; adaptive immunity; Bovine orthopneumovirus; feedlots; respiratory tract diseases; Human orthopneumovirus; bovine respiratory disease; children; mortality; viruses; immune response; lungs; calves; chemokines; animal age; infants
Abstract:
... γδ T cells are a subset of nonconventional T cells that play a critical role in bridging the innate and adaptive arms of the immune system. γδ T cells are particularly abundant in ruminant species and may constitute up to 60% of the circulating lymphocyte pool in young cattle. The frequency of circulating γδ T cells is highest in neonatal calves and declines as the animal ages, suggesting these ce ...
Handle:
10113/63139
DOI:
10.1016/j.vetimm.2016.02.012
CHORUS:
10.1016/j.vetimm.2016.02.012
Chorus Open Access:
10.1016/j.vetimm.2016.02.012

87. Activation of bovine neutrophils by Brucella spp

Author:
Lauren L. Keleher; Jerod A. Skyberg
Source:
Veterinary immunology and immunopathology 2016 v.177 pp. 1-6
ISSN:
0165-2427
Subject:
Brucella melitensis biovar Abortus; Brucella melitensis biovar Canis; Brucella melitensis biovar Suis; Gram-negative bacteria; brucellosis; cattle; death; humans; neutrophils; pathogens; virulence; virulent strains
Abstract:
... Brucellosis is a globally important zoonotic infectious disease caused by gram negative bacteria of the genus Brucella. While many species of Brucella exist, Brucella melitensis, Brucella abortus, and Brucella suis are the most common pathogens of humans and livestock. The virulence of Brucella is largely influenced by its ability to evade host factors, including phagocytic killing mechanisms, whi ...
DOI:
10.1016/j.vetimm.2016.05.011

88. Assessment of reference genes for reliable analysis of gene transcription by RT-qPCR in ovine leukocytes

Author:
T.A.N. Mahakapuge; J.-P.Y. Scheerlinck; C.A. Alvarez Rojas; A.L. Every; J. Hagen
Source:
Veterinary immunology and immunopathology 2016 v.171 pp. 1-6
ISSN:
0165-2427
Subject:
algorithms; genes; lymph; mononuclear leukocytes; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; sheep; transcription (genetics)
Abstract:
... With the availability of genetic sequencing data, quantitative reverse transcription PCR (RT-qPCR) is increasingly being used for the quantification of gene transcription across species. Too often there is little regard to the selection of reference genes and the impact that a poor choice has on data interpretation. Indeed, RT-qPCR provides a snapshot of relative gene transcription at a given time ...
DOI:
10.1016/j.vetimm.2015.10.010

89. Bovine WC1+ γδ T lymphocytes modify monocyte-derived macrophage responses during early Mycobacterium avium subspecies paratuberculosis infection

Author:
Monica M. Baquero; Brandon L. Plattner
Source:
Veterinary immunology and immunopathology 2016 v.170 pp. 65-72
ISSN:
0165-2427
Subject:
Mycobacterium avium subsp. paratuberculosis; T-lymphocytes; calves; coculture; direct contact; immune response; innate immunity; interferon-gamma; interleukin-10; interleukin-17; interleukin-4; macrophages; nitrites; pathogens; synergism; viability
Abstract:
... Following Mycobacterium avium subspecies paratuberculosis (Map) infection, some calves are apparently able to successfully clear the pathogen whereas others become persistently infected; however the reasons for this remain unknown. The importance of innate immunity, and in particular the role of γδ T lymphocytes, during early anti-mycobacterial immune response is recognized but specific mechanisms ...
DOI:
10.1016/j.vetimm.2015.12.002

90. Butyric acid stimulates bovine neutrophil functions and potentiates the effect of platelet activating factor

Author:
M.D. Carretta; A.I. Hidalgo; J. Burgos; L. Opazo; L. Castro; M.A. Hidalgo; C.D. Figueroa; A. Taubert; C. Hermosilla; R.A. Burgos
Source:
Veterinary immunology and immunopathology 2016 v.176 pp. 18-27
ISSN:
0165-2427
Subject:
agonists; butyric acid; calcium; cattle; chemotaxis; digestive system; free fatty acids; gelatinase B; humans; inflammation; innate immunity; lactoferrin; mitogen-activated protein kinase; neutrophils; phospholipase C; phosphorylation; platelet-activating factor; reactive oxygen species; rodents; ruminal acidosis; second messengers; viability
Abstract:
... Increased short-chain fatty acid (SCFA) production is associated with subacute ruminal acidosis (SARA) and activation of inflammatory processes. In humans and rodents, SCFAs modulate inflammatory responses in the gut via free fatty acid receptor 2 (FFA2). In bovines, butyric acid is one of the most potent FFA2 agonists. Its expression in bovine neutrophils has recently been demonstrated, suggestin ...
DOI:
10.1016/j.vetimm.2016.05.002

91. Cancer immunology and canine malignant melanoma: A comparative review

Author:
Matthew J. Atherton; Joanna S. Morris; Mark R. McDermott; Brian D. Lichty
Source:
Veterinary immunology and immunopathology 2016 v.169 pp. 15-26
ISSN:
0165-2427
Subject:
disease models; dogs; humans; immune response; immune system; medical treatment; melanoma; oral administration; plasmids; recombinant vaccines; vaccination
Abstract:
... Oral canine malignant melanoma (CMM) is a spontaneously occurring aggressive tumour with relatively few medical treatment options, which provides a suitable model for the disease in humans. Historically, multiple immunotherapeutic strategies aimed at provoking both innate and adaptive anti-tumour immune responses have been published with varying levels of activity against CMM. Recently, a plasmid ...
DOI:
10.1016/j.vetimm.2015.11.003

92. Cellular endocytic compartment localization of expressed canine CD1 molecules

Author:
Mette Schjaerff; Stefan M. Keller; Verena K. Affolter; Annemarie T. Kristensen; Peter F. Moore
Source:
Veterinary immunology and immunopathology 2016 v.182 pp. 11-21
ISSN:
0165-2427
Subject:
T-lymphocytes; antigens; confocal microscopy; dendritic cells; dogs; endosomes; genes; glycoproteins; humans; immunity; lipids; lysosomes; plasma membrane; protein synthesis; surveys
Abstract:
... CD1 molecules are glycoproteins present primarily on dendritic cells (DCs), which recognize and present a variety of foreign- and self-lipid antigens to T-cells. Humans have five different CD1 isoforms that survey distinct cellular compartments allowing for recognition of a large repertoire of lipids. The canine CD1 family consists of seven functional CD1 molecules (canine CD1a2, CD1a6, CD1a8, CD1 ...
DOI:
10.1016/j.vetimm.2016.08.012

93. Characterization and use of new monoclonal antibodies to CD11c, CD14, and CD163 to analyze the phenotypic complexity of ruminant monocyte subsets

Author:
Mahmoud M. Elnaggar; Gaber S. Abdellrazeq; Victoria Mack; Lindsay M. Fry; William C. Davis; Kun Taek Park
Source:
Veterinary immunology and immunopathology 2016 v.178 pp. 57-63
ISSN:
0165-2427
Subject:
cattle; epitopes; flow cytometry; gene expression regulation; genome; goats; granulocytes; mass spectrometry; monoclonal antibodies; monocytes; phenotype; sheep
Abstract:
... The sequencing of the bovine genome and development of mass spectrometry, in conjunction with flow cytometry (FC), have afforded an opportunity to complete the characterization of the specificity of monoclonal antibodies (mAbs), only partially characterized during previous international workshops focused on antibody development for livestock (1991, Leukocyte Antigens in Cattle, Sheep, and Goats; 1 ...
DOI:
10.1016/j.vetimm.2016.06.010

94. Comparison of the immune competence of Turopolje, German Landrace × Turopolje, and German Landrace × Pietrain pigs after PRRSV vaccination

Author:
I.C. Ballweg; K. Frölich; E. Fandrey; H. Kliem; M.W. Pfaffl
Source:
Veterinary immunology and immunopathology 2016 v.174 pp. 35-44
ISSN:
0165-2427
Subject:
German Landrace; Pietrain; Porcine reproductive and respiratory syndrome virus; Toll-like receptor 8; antibodies; antibody formation; blood serum; enzyme-linked immunosorbent assay; gene expression; genetic markers; immunocompetence; interleukin-12; interleukin-2; interleukin-6; leukocytes; live vaccines; porcine reproductive and respiratory syndrome; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; swine; tonsils; tumor necrosis factor-alpha; vaccination; viruses
Abstract:
... The competences of the immune systems of the ancient pig breed Turopolje (T×T), German Landrace × Turopolje (L×T) and ‘modern’ pig breed German Landrace × Pietrain (L×P) were compared in this study. All pigs were immunized with a modified live vaccine against ‘Porcine Reproductive and Respiratory Syndrome’ (PRRS) virus (Ingelvac PRRS MLV®) to simulate an infection. Antibody production against PRRS ...
DOI:
10.1016/j.vetimm.2016.04.003

95. Cytokine and chemokine profiles of aqueous humor and serum in horses with uveitis measured using multiplex bead immunoassay analysis

Author:
Elizabeth Curto; Kristen M. Messenger; Jacklyn H. Salmon; Brian C. Gilger
Source:
Veterinary immunology and immunopathology 2016 v.182 pp. 43-51
ISSN:
0165-2427
Subject:
DNA; antibodies; blood serum; chemokine CCL5; chemokine CX3CL1; eyes; granulocyte colony-stimulating factor; horses; immunoassays; inflammation; interferon-gamma; interleukin-10; interleukin-12; interleukin-13; interleukin-17; interleukin-4; interleukin-6; interleukin-8; keratitis; leptospirosis; ophthalmology; polymerase chain reaction
Abstract:
... To determine whether horses with clinically diagnosed Equine Recurrent Uveitis (ERU) and those with Leptospirosis infection have a specific cytokine profile in their aqueous humor (AH) and serum that differs from horses with uveitis secondary to other ocular inflammatory processes and from horses with normal eyes.Twenty-five client-owned horses with uveitis that were presented to the North Carolin ...
DOI:
10.1016/j.vetimm.2016.09.008

96. Demonstration of the ability of a canine Lyme vaccine to reduce the incidence of histological synovial lesions following experimentally-induced canine Lyme borreliosis

Author:
Deborah A. Grosenbaugh; Daniel R. Rissi; Paula M. Krimer
Source:
Veterinary immunology and immunopathology 2016 v.180 pp. 29-33
ISSN:
0165-2427
Subject:
Beagle; Borrelia burgdorferi; Ixodes scapularis; Lyme disease; Western blotting; antigens; biopsy; disease vectors; dog diseases; dogs; histopathology; lameness; models; polymerase chain reaction; serology; surface proteins; ticks; vaccination; vaccines
Abstract:
... Lyme disease in dogs can be effectively prevented by vaccination against antigens expressed by the spirochete Borrelia burgdorferi during transmission by the tick vector Ixodes sp. Lyme vaccine efficacy has traditionally been based on indicators of infection following wild-caught tick challenge whereas most other types of vaccine are required to demonstrate protection from clinical signs of diseas ...
DOI:
10.1016/j.vetimm.2016.08.014

97. Development of an autologous canine cancer vaccine system for resectable malignant tumors in dogs

Author:
J.R. Yannelli; R. Wouda; T.J. Masterson; M.G. Avdiushko; D.A. Cohen
Source:
Veterinary immunology and immunopathology 2016 v.182 pp. 95-100
ISSN:
0165-2427
Subject:
Western blotting; adjuvants; antigens; biopsy; blood sampling; cell death; cell suspension culture; dogs; endotoxins; immune response; immunoglobulin G; immunotherapy; neoplasm cells; neoplasms; oligodeoxyribonucleotides; patients; ultraviolet radiation; vaccines; veterinary clinics
Abstract:
... While conventional therapies exist for canine cancer, immunotherapies need to be further explored and applied to the canine setting. We have developed an autologous cancer vaccine (K9-ACV), which is available for all dogs with resectable disease. K9-ACV was evaluated for safety and immunogenicity for a variety of cancer types in a cohort of companion dogs under veterinary care. The autologous vacc ...
DOI:
10.1016/j.vetimm.2016.10.011

98. Development of recombinant BgP12 based enzyme linked immunosorbent assays for serodiagnosis of Babesia gibsoni infection in dogs

Author:
Mrityunjay Mandal; Partha S. Banerjee; Saroj Kumar; Rajat Garg; Hira Ram; Opinder K. Raina
Source:
Veterinary immunology and immunopathology 2016 v.169 pp. 27-33
ISSN:
0165-2427
Subject:
Babesia canis subsp. vogeli; Babesia gibsoni; Dirofilaria immitis; Ehrlichia canis; Escherichia coli; Hepatozoon canis; Western blotting; blood serum; cross reaction; dog diseases; dogs; enzyme-linked immunosorbent assay; epidemiological studies; erythrocytes; genes; open reading frames; polymerase chain reaction; rabbits; serodiagnosis
Abstract:
... Indirect ELISA and dot-ELISA using recombinant BgP12 (rBgP12) were developed for the diagnosis of Babesia gibsoni infected dogs. The complete open reading frame of BgP12 gene (378bp) was cloned in pET-32a(+) expression vector and expressed in Escherichia coli as a soluble thioredoxin (Trx) fusion protein. The purified rBgP12 was used for production of anti-rBgP12 rabbit serum, which recognized a n ...
DOI:
10.1016/j.vetimm.2015.10.013

99. Development of single dilution immunoassay to detect E2 protein specific classical swine fever virus antibody

Author:
Rakesh Kumar; Nagendra N. Barman; Elina Khatoon; Sachin Kumar
Source:
Veterinary immunology and immunopathology 2016 v.172 pp. 50-54
ISSN:
0165-2427
Subject:
Classical swine fever virus; antibodies; blood serum; border disease; bovine viral diarrhea; cost effectiveness; diagnostic techniques; endemic diseases; enzyme-linked immunosorbent assay; immunity; surface proteins; swine; vaccination; viral antigens; viruses
Abstract:
... Classical swine fever virus (CSFV) is the causative agent of a highly contagious disease in swine. The disease is endemic in different parts of the world and vaccination is the only way to protect pigs from CSFV infection. The virus surface protein E2 is the major immunogenic protein eliciting protective immunity against CSFV infection in swine. The whole virus antigen cannot differentiate CSFV fr ...
DOI:
10.1016/j.vetimm.2016.03.004

100. An ELISA for quantifying quail IgY and characterizing maternal IgY transfer to egg yolk in several quail strains

Author:
Atsushi Murai; Misako Kakiuchi; Takahito Hamano; Misato Kobayashi; Masaoki Tsudzuki; Mikiharu Nakano; Yoichi Matsuda; Fumihiko Horio
Source:
Veterinary immunology and immunopathology 2016 v.175 pp. 16-23
ISSN:
0165-2427
Subject:
blood; egg yolk; eggs; enzyme-linked immunosorbent assay; immunoglobulin Y; oocytes; polyclonal antibodies; quails; rabbits; strain differences
Abstract:
... In avian species, maternal blood immunoglobulin Y (IgY) is transferred to the egg yolks of maturing oocytes, but the mechanism underlying this transfer is unknown. To gain insight into the mechanism of maternal IgY transfer in quail, we established an enzyme-linked immunosorbent assay (ELISA) for the quantitation of quail IgY. We characterized strain differences in blood and egg yolk IgY concentra ...
DOI:
10.1016/j.vetimm.2016.04.013