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You searched for: Journal name Veterinary immunology and immunopathology Remove constraint Journal name: Veterinary immunology and immunopathology Publication year rev 7990-2010 Remove constraint Publication year rev: 7990-2010 Source 2010 v.137 no.3-4 Remove constraint Source: 2010 v.137 no.3-4
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1. Aberrant autophosphorylation of c-Kit receptor in canine mast cell tumor cell lines

Author:
Takeuchi, Yoshinori; Fujino, Yasuhito; Watanabe, Manabu; Nakagawa, Takayuki; Ohno, Koichi; Sasaki, Nobuo; Sugano, Sumio; Tsujimoto, Hajime
Source:
Veterinary immunology and immunopathology 2010 v.137 no.3-4 pp. 208-216
ISSN:
0165-2427
Subject:
pathophysiology; neoplasms; dog diseases; dogs; mutagenesis; skin diseases; biochemical pathways; stem cell factor; amino acids; gene expression; cell physiology; genetic disorders; mast cells; phosphorylation; pathogenesis; receptors; cell lines; immune response; in vitro studies
Abstract:
... Several studies indicated that KIT mutation could cause ligand-independent activation of c-Kit receptor in canine mast cell tumor (MCT). The objective of this study was to investigate mechanisms of c-Kit receptor activation in various canine MCT cell lines. Four cell lines, HRMC (derived from cutaneous MCT), VIMC1 (visceral MCT), CoMS1 (visceral MCT) and CMMC1 (cutaneous MCT), were cultured in ste ...
DOI:
10.1016/j.vetimm.2010.05.009

2. Canine leishmaniosis. Immunophenotypic profile of leukocytes in different compartments of symptomatic, asymptomatic and treated dogs

Author:
Alexandre-Pires, Graça; de Brito, Maria Teresa Villa; Algueró, Cármen; Martins, Catarina; Rodrigues, Olivia Roos; da Fonseca, Isabel Pereira; Santos-Gomes, Gabriela
Source:
Veterinary immunology and immunopathology 2010 v.137 no.3-4 pp. 275-283
ISSN:
0165-2427
Subject:
phenotype; disease course; CD8-positive T-lymphocytes; dog diseases; dogs; Leishmania infantum; major histocompatibility complex; visceral leishmaniasis; bone marrow; disease severity; disease resistance; immune response; lymph nodes; pathogenesis; CD4-positive T-lymphocytes
Abstract:
... Canine visceral leishmaniosis (CanL) is an emerging disease, expanding in various parts of the world. The infection caused by Leishmania, an intracellular protozoan parasite, can show different clinical manifestations, from asymptomatic or subclinical to symptomatic dogs, in which a wide spectrum of clinical signs is evident. The fact that the parasite replicates in different organs raises the hyp ...
DOI:
10.1016/j.vetimm.2010.06.007

3. Clathrin-mediated endocytosis and transcytosis of enterotoxigenic Escherichia coli F4 fimbriae in porcine intestinal epithelial cells

Author:
Rasschaert, Kristien; Devriendt, Bert; Favoreel, Herman; Goddeeris, Bruno M.; Cox, Eric
Source:
Veterinary immunology and immunopathology 2010 v.137 no.3-4 pp. 243-250
ISSN:
0165-2427
Subject:
protective effect; fimbriae; diarrhea; swine diseases; epithelial cells; mucosal immunity; physiological transport; receptors; biochemical pathways; endocytosis; enterotoxigenic Escherichia coli; intestines; immunization; in vitro studies; clathrin; epithelium; oral administration; Escherichia infections; swine
Abstract:
... Enterotoxigenic Escherichia coli (ETEC) cause severe diarrhea in neonatal and recently weaned piglets. Previously, we demonstrated that oral immunization of F4 receptor positive piglets with purified F4 fimbriae induces a protective F4-specific intestinal immune response. However, in F4 receptor negative animals no F4-specific immune response can be elicited, indicating that the induction of an F4 ...
DOI:
10.1016/j.vetimm.2010.05.016

4. Cloning, expression and bioactivity of BAFF from Petaurus breviceps

Author:
Ren, Wen-hua; Xu, Ying; Zhang, Shuang-quan; Yang, Guang
Source:
Veterinary immunology and immunopathology 2010 v.137 no.3-4 pp. 332-336
ISSN:
0165-2427
Subject:
interspecific variation; complementary DNA; open reading frames; humans; bioactive properties; phylogeny; amino acid sequences; gene expression; Metatheria; molecular cloning; tumor necrosis factors; lymphocyte proliferation; sequence homology; animal genetics; B-lymphocytes
Abstract:
... B-cell activating factor (BAFF), belonging to the TNF (tumor necrosis factor) family, is crucial for B-cell survival and maturation. In the present study, PbBAFF cDNA was amplified from the sugar glider Petaurus breviceps by RT-PCR and RACE (rapid amplification of cDNA ends) strategies. The open reading frame (ORF) of PbBAFF cDNA encodes a protein consisting of 287-amino acid. The deduced amino ac ...
DOI:
10.1016/j.vetimm.2010.06.003

5. Distinct reactivities of interleukin-4-specific antibodies with recombinant and native canine interleukin-4 in various assays

Author:
Lauber, Beatrice; Marti, Eliane; Plattet, Philippe; Urwyler, Adrian; Rai, Muhammad Farooq; Schmidt, Michael F.G.; Welle, Monika; Zurbriggen, Andreas; Janda, Jozef
Source:
Veterinary immunology and immunopathology 2010 v.137 no.3-4 pp. 310-316
ISSN:
0165-2427
Subject:
cross reaction; hypersensitivity; dog diseases; parasitoses; dogs; biomarkers; antibodies; allergens; antibody detection; Western blotting; enzyme-linked immunosorbent assay; differential staining; interleukin-4
Abstract:
... Interleukin 4 (IL-4) plays a central role in immune responses to parasites and allergens. IL-4 drives the differentiation of naive T cells into Th2 cells and regulates immunoglobulin class switching to IgE.Little is known about the role of IL-4 in canine allergies and parasite infections. Most of the information derives from measurement of IL-4 mRNA expression in dog tissues, but detection of IL-4 ...
DOI:
10.1016/j.vetimm.2010.05.015

6. Effect of IL-12 on canine dendritic cell maturation following differentiation induced by granulocyte-macrophage CSF and IL-4

Author:
Sugiura, Kikuya; Wijewardana, Viskam; Fujimoto, Mariko; Akazawa, Takashi; Yahata, Mana; Mito, Kai; Hatoya, Shingo; Inoue, Norimitsu; Inaba, Toshio
Source:
Veterinary immunology and immunopathology 2010 v.137 no.3-4 pp. 322-326
ISSN:
0165-2427
Subject:
monocytes; neoplasms; dendritic cells; dogs; macrophages; T-lymphocytes; cytotoxicity; granulocytes; immune response; in vitro studies; pathogenesis; cell differentiation; interleukin-4; interleukin-12
Abstract:
... IL-12 is a cytokine produced by dendritic cells (DC) and activates cytotoxic T cells and NK cells against tumors. We investigated the effect of IL-12 on DC. When peripheral blood monocytes were incubated with canine granulocyte-macrophage (GM)-CSF and IL-4, expression of MHC class II, CD1a, CD80 and CD86 was significantly elevated, but the cell morphology was that of immature DC. By adding canine ...
DOI:
10.1016/j.vetimm.2010.06.006

7. Generation and characterization of anti-α-enolase single-chain antibodies in chicken

Author:
Leu, Sy-Jye; Lee, Yu-Ching; Shih, Neng-Yao; Huang, I-Jen; Liu, Ko-Jiunn; Lu, Hsu-Feng; Huang, Shih-Yi; Yang, Yi-Yuan
Source:
Veterinary immunology and immunopathology 2010 v.137 no.3-4 pp. 251-260
ISSN:
0165-2427
Subject:
chickens; phosphopyruvate hydratase; immunoglobulin Y; antibody formation; neoplasms; polyclonal antibodies; physiological regulation; immunotherapy; poultry diseases; mutation; bacteriophages; clones; monoclonal antibodies; enzyme activity; antigen-antibody reactions; disease diagnosis
Abstract:
... It was previously reported that up-regulation of α-enolase protein was detected in 65% of patients with non-small cell lung cancers (NSCLC). Moreover, a high titer of anti-α-enolase antibodies was developed in a smaller proportion (7.4%) of these patients than in non-tumor-associated patients and healthy subjects. In the present study, we characterized polyclonal and single-chain variable fragment ...
DOI:
10.1016/j.vetimm.2010.06.001
PubMed:
20655599
PubMed Central:
PMC7112641

8. In vitro and in vivo evaluation of five low molecular weight proteins of Ehrlichia ruminantium as potential vaccine components

Author:
Sebatjane, S.I.; Pretorius, A.; Liebenberg, J.; Steyn, H.; Van Kleef, M.
Source:
Veterinary immunology and immunopathology 2010 v.137 no.3-4 pp. 217-225
ISSN:
0165-2427
Subject:
in vivo studies; biosynthesis; in vitro studies; Ehrlichia ruminantium; open reading frames; sheep; recombinant proteins; T-lymphocytes; heartwater; sheep diseases; bacterial antigens; genes; vaccination; vaccine development; molecular weight; lymphocyte proliferation; subunit vaccines; interferons
Abstract:
... Low molecular weight (LMW) proteins of E. ruminantium can induce proliferation of immune peripheral blood mononuclear cells (PBMCs) and the production of interferon-gamma (IFN-γ) by CD4+-enriched T-cells. In this study, a reverse vaccinology approach was applied to identify additional vaccine candidates focusing on genes that encode LMW proteins smaller than 20kDa. Five open reading frames (ORFs) ...
DOI:
10.1016/j.vetimm.2010.05.011

9. Molecular cloning, characterization and in vitro expression of SERPIN B1 of bighorn sheep (Ovis canadensis) and domestic sheep (Ovis aries), and comparison with that of other species

Author:
Subramaniam, Renuka; Dassanayake, Rohana P.; Norimine, Junzo; Brown, Wendy C.; Knowles, Donald P.; Srikumaran, Subramaniam
Source:
Veterinary immunology and immunopathology 2010 v.137 no.3-4 pp. 327-331
ISSN:
0165-2427
Subject:
serine proteinases; proteinase inhibitors; Ovis canadensis; sheep; molecular cloning; Mannheimia haemolytica; amino acid sequences; nucleotide sequences
Abstract:
... Mannheimia haemolytica infection results in enhanced PMN-mediated tissue damage in the lungs of bighorn sheep (BHS) compared to that of domestic sheep (DS). SERPIN B1 is an inhibitor of PMN-derived serine proteases. It prevents lung tissue injury by inhibiting the serine proteases released as a result of PMN lysis and degranulation. It is conceivable that PMNs of BHS exhibit decreased quantity and ...
Handle:
10113/45731
DOI:
10.1016/j.vetimm.2010.05.017

10. Phenotypical characterization, distribution and quantification of different mast cell subtypes in transmural biopsies from the gastrointestinal tract of cats with inflammatory bowel disease

Author:
Kleinschmidt, Sven; Harder, Jasmine; Nolte, Ingo; Marsilio, Sina; Hewicker-Trautwein, Marion
Source:
Veterinary immunology and immunopathology 2010 v.137 no.3-4 pp. 190-200
ISSN:
0165-2427
Subject:
phenotype; immunohistochemistry; cats; enzymes; enterocolitis; gastric mucosa; cytokines; tissue distribution; disease severity; pathogenesis; cat diseases; mast cells; immune response; enzyme activity; intestinal mucosa; biopsy
Abstract:
... In this study subtypes, distribution and number of mast cells were investigated within mucosa and submucosa of the gastrointestinal tract of 24 cats with inflammatory bowel disease (IBD) in comparison to 11 control cats. Paraffin sections of formalin-fixed transmural gastrointestinal biopsies from stomach, duodenum, jejunum, ileum and colon were examined. Mast cells were phenotyped and quantified ...
DOI:
10.1016/j.vetimm.2010.05.005

11. Porcine circovirus type 2 (PCV2) Cap and Rep proteins are involved in the development of cell-mediated immunity upon PCV2 infection

Author:
Fort, Maria; Sibila, Marina; Nofrarías, Miquel; Pérez-Martín, Eva; Olvera, Alex; Mateu, Enric; Segalés, Joaquim
Source:
Veterinary immunology and immunopathology 2010 v.137 no.3-4 pp. 226-234
ISSN:
0165-2427
Subject:
strains; antigenic variation; disease course; enzymes; cell-mediated immunity; viremia; virulence; disease severity; gene expression; bacterial antigens; pathogenesis; genes; pathotypes; Porcine circovirus; microbial genetics; virus replication; postweaning multisystemic wasting syndrome; swine
Abstract:
... The aim of the present study was to investigate the role of the capside (Cap) and replicase (Rep) proteins of Porcine circovirus type 2 (PCV2) as well as the whole PCV2 (both PCV2a and PCV2b genotypes) in the induction of cell-mediated immunity upon infection. At 6 weeks of age, six pigs were intranasally inoculated with the Stoon 1010 (Stoon) isolate (PCV2a) and seven with the Sp-7-10-54-13 (Sp) ...
DOI:
10.1016/j.vetimm.2010.05.013

12. Ultra-early weaning in piglets results in low serum IgA concentration and IL17 mRNA expression

Author:
Levast, Benoît; de Monte, Michèle; Chevaleyre, Claire; Melo, Sandrine; Berri, Mustapha; Mangin, Françoise; Zanello, Galliano; Lantier, Isabelle; Salmon, Henri; Meurens, François
Source:
Veterinary immunology and immunopathology 2010 v.137 no.3-4 pp. 261-268
ISSN:
0165-2427
Subject:
piglets; early weaning; risk assessment; adverse effects; immune response; immunoglobulin A; interleukins; blood serum; chemical concentration; messenger RNA; transcriptome; gene expression; biosynthesis; intestines; mucosal immunity; animal age
Abstract:
... In pigs raised for meat production, weaning is a critical period because of related physiological perturbations and negative consequences on performance. Previous studies have shown that early weaning could either impair development of mucosal barrier function or boost intestinal immunologic parameters. In order to obtain further knowledge about the impact of ultra-early weaning on the porcine imm ...
DOI:
10.1016/j.vetimm.2010.06.004

13. Validation of endogenous reference genes for RT-qPCR normalisation in bovine lymphoid cells (BL-3) infected with Bovine Viral Diarrhoea Virus (BVDV)

Author:
Anstaett, Olivia L.; Brownlie, Joe; Collins, Margaret E.; Thomas, Carole J.
Source:
Veterinary immunology and immunopathology 2010 v.137 no.3-4 pp. 201-207
ISSN:
0165-2427
Subject:
lymphatic system; quantitative analysis; animal proteins; cattle; cell culture; oxygenases; reverse transcriptase polymerase chain reaction; validity; bovine viral diarrhea; gene expression; Bovine viral diarrhea virus 1; disease resistance; immune response; digestive system diseases; pathogenesis; genes; cell lines; globulins; in vitro studies
Abstract:
... Reverse transcription quantitative PCR (RT-qPCR) is a highly sensitive tool that can be used for accurate and reliable gene expression analysis; however, careful normalisation to a set of stably expressed endogenous reference genes is essential. Expression levels of many reference genes in RT-qPCR analyses can be extremely variable under different experimental conditions, producing potentially err ...
DOI:
10.1016/j.vetimm.2010.05.006

14. A selective high affinity ligand (SHAL) designed to bind to an over-expressed human antigen on non-Hodgkin's lymphoma also binds to canine B-cell lymphomas

Author:
Balhorn, Rod L.; Skorupski, Katherine A.; Hok, Saphon; Balhorn, Monique Cosman; Guerrero, Teri; Rebhun, Robert B.
Source:
Veterinary immunology and immunopathology 2010 v.137 no.3-4 pp. 235-242
ISSN:
0165-2427
Subject:
epitopes; gene overexpression; dog diseases; antibodies; dogs; immunotherapy; cytotoxicity; outer membrane proteins; major histocompatibility complex; non-Hodgkin lymphoma; in vitro studies; binding capacity; antigen-antibody reactions; B-lymphocytes
Abstract:
... Therapies using antibodies directed against cell surface proteins have improved survival for human patients with non-Hodgkin's lymphoma (NHL). It is possible that similar immuno-therapeutic approaches may also benefit canine NHL patients. Unfortunately, variability between human and canine epitopes often limits the usefulness of such therapies in pet dogs. The Lym-1 antibody recognizes a unique ep ...
DOI:
10.1016/j.vetimm.2010.05.014

15. Cellular immune responses in cetaceans immunized with a porcine erysipelas vaccine

Author:
Sitt, Tatjana; Bowen, Lizabeth; Blanchard, Myra T.; Gershwin, Laurel J.; Byrne, Barbara A.; Dold, Christopher; McBain, Jim; Stott, Jeffrey L.
Source:
Veterinary immunology and immunopathology 2010 v.137 no.3-4 pp. 181-189
ISSN:
0165-2427
Subject:
dolphins; Tursiops truncatus; captive animals; zoo animals; skin diseases; bacterial infections; Erysipelothrix rhusiopathiae; vaccination; subunit vaccines; safety testing; protective effect; dosage; in vitro studies; cell-mediated immunity; T-lymphocytes; interferons; interleukins; antigen-antibody reactions
Abstract:
... Clinical erysipelas represents a significant health problem in managed cetacean species. Vaccination was suspended in many oceanariums in the past due to losses associated with vaccine-induced hypersensitivities which were deemed to be a greater threat than clinical erysipelas. A perceived shift in clinical presentation of erysipelas from a chronic dermatologic form to an acute systemic form in do ...
DOI:
10.1016/j.vetimm.2010.05.003

16. Genetic characterization and ligand specificity of the ovine Fc gamma receptor I (ovFcγRІ)

Author:
Qiao, Songlin; Liu, Yunchao; Zhang, Jiuliang; Yang, Suzhen; Wan, Bo; Shi, Pingling; Zhang, Hong; Guo, Junqing; Zhang, Gaiping
Source:
Veterinary immunology and immunopathology 2010 v.137 no.3-4 pp. 317-321
ISSN:
0165-2427
Subject:
immunoglobulin G; messenger RNA; complementary DNA; open reading frames; humoral immunity; sheep; amino acid sequences; gene expression; disease resistance; molecular cloning; receptors; sequence homology; infection
Abstract:
... Receptors for the Fc region of IgG (FcγRs) play a critical role in the immune system and host protection against infection. In this study, we describe the cloning, mRNA expression and IgG subclass specificity of ovine Fc gamma receptor I (ovFcγRI). The ovFcγRI cDNA contains a 1047bp open-reading frame, and is predicted to encode a 349 amino acid trans-membrane glycoprotein composed of three immuno ...
DOI:
10.1016/j.vetimm.2010.06.002

17. Immunohistochemical characterization of selected cell markers for the detection of hematopoietic cells in formalin-fixed, paraffin wax-embedded lymphoid tissues of harbor seals (Phoca vitulina) and walruses (Odobenus rosmarus rosmarus)

Author:
Seibel, H.; Stimmer, L.; Siebert, U.; Beineke, A.
Source:
Veterinary immunology and immunopathology 2010 v.137 no.3-4 pp. 305-309
ISSN:
0165-2427
Subject:
cross reaction; Odobenus rosmarus; disease detection; lymphatic system; interspecific variation; phenotype; antigens; immunohistochemistry; polyclonal antibodies; lymphocytes; inflammation; validity; Phoca vitulina; monoclonal antibodies; lymphocyte count
Abstract:
... To facilitate a detailed investigation of pinniped lymphoid organs, 30 monoclonal antibodies (mAb) as well as eight polyclonal antibodies (pAb) of different species specificities directed against cell antigens of the hematopoietic system were tested for immunohistochemical cross-reactivity on formalin-fixed, paraffin wax-embedded tissues of harbor seals (Phoca vitulina) and a walrus (Odobenus rosm ...
DOI:
10.1016/j.vetimm.2010.05.012

18. Immunohistochemical investigation of cells expressing CD21, membrane IgM, CD32 and a follicular dendritic cell marker in the lymphoid tissues of neonatal calves

Author:
Chattha, Kuldeep S.; Hodgins, Douglas C.; DeLay, Josepha; Antoine, Nadine; Shewen, Patricia E.
Source:
Veterinary immunology and immunopathology 2010 v.137 no.3-4 pp. 284-290
ISSN:
0165-2427
Subject:
immunoglobulin M; complement; antibody formation; immunohistochemistry; dendritic cells; maternal immunity; antibodies; spleen; neonates; receptors; calves; lymph nodes; animal age; B-lymphocytes
Abstract:
... Activation of B lymphocytes in the presence of passive maternal antibodies depends on expression of CD21, membrane IgM and CD32. On colligation with IgM, CD32 inhibits activation whereas CD21 enhances it. Recently, we assessed expression of CD21 and CD32 on IgM⁺ cells from lymphoid tissues of newborn calves by flow cytometry, but this approach does not provide information about spatial distributio ...
DOI:
10.1016/j.vetimm.2010.05.004

19. Oral administration of beta-1,3/1,6-glucan Macrogard® fails to enhance the mucosal immune response following oral F4 fimbrial immunisation in gnotobiotic pigs

Author:
Stuyven, E.; Van den Broeck, W.; Nauwynck, H.; Goddeeris, B.M.; Cox, E.
Source:
Veterinary immunology and immunopathology 2010 v.137 no.3-4 pp. 291-297
ISSN:
0165-2427
Subject:
fimbriae; oral administration; dose response; swine diseases; interleukin-4; mucosal immunity; dosage; germ-free animals; immunoglobulin A; bacterial antigens; enterotoxigenic Escherichia coli; spleen; prebiotics; immunization; digestive system diseases; immunostimulants; beta-glucans; Escherichia infections; swine
Abstract:
... In this study gnotobiotic animals were used to see if the F4 fimbrial antigen of F4ac+ Escherichia coli is as immunogenic as in conventional pigs. In addition, the adjuvant effect of beta-1,3/1,6-glucans was analysed for the first time in germ-free domestic animals. Oral F4 immunisation of F4 receptor positive (F4R⁺) piglets was able to induce a systemic and mucosal immune response as conventional ...
DOI:
10.1016/j.vetimm.2010.05.007

20. Seroepidemiology of porcine enteric sapovirus in pig farms in Venezuela

Author:
Alcalá, Ana C.; Rodríguez-Díaz, Jesús; de Rolo, Morella; Vizzi, Esmeralda; Buesa, Javier; Liprandi, Ferdinando; Ludert, Juan E.
Source:
Veterinary immunology and immunopathology 2010 v.137 no.3-4 pp. 269-274
ISSN:
0165-2427
Subject:
antigenic variation; animal age; diarrhea; seroprevalence; viral diseases of animals and humans; swine diseases; biomarkers; Porcine enteric calicivirus; risk assessment; epidemiological studies; coat proteins; capsid; antibody detection; immune response; maternal immunity; microbial genetics; swine; etiology; virion; disease prevalence; Venezuela
Abstract:
... Porcine enteric sapovirus (PES) has been shown to cause diarrhea under experimental conditions in gnotobiotic piglets. However, the role of PES as enteric pathogens in porcine farms remains unclear. To further understand the PES-host interactions under field conditions, a serological survey was carried out. To this end the capsid gene of a PES isolate was cloned in the baculovirus expression syste ...
DOI:
10.1016/j.vetimm.2010.06.005