PubAg

Main content area

You searched for: Journal Veterinary immunology and immunopathology Remove constraint Journal: Veterinary immunology and immunopathology Publication Year 2006 Remove constraint Publication Year: 2006
Start Over

Search

194  Search Results

| 21 - 40 of 194 |
Number of results to display per page

Search Results

21. Cross-reactive cellular and humoral immune responses to Salmonella enterica serovars Typhimurium and Enteritidis are associated with protection to heterologous re-challenge

Author:
Beal, R.K.; Wigley, P.; Powers, C.; Barrow, P.A.; Smith, A.L.
Source:
Veterinary immunology and immunopathology 2006 v.114 no.1-2 pp. 84-93
ISSN:
0165-2427
Subject:
chickens; food animals; Salmonella enteritidis; Salmonella enterica subsp. enterica serovar Typhimurium; food pathogens; food contamination; cell-mediated immunity; humoral immunity; immune response; cross reaction; vaccination; salmonellosis; bacterial contamination; serotypes; enzyme-linked immunosorbent assay; splenocytes; cell proliferation; lymphocyte proliferation; immunoglobulin A; immunoglobulin G; immunoglobulin M; T-lymphocytes; live vaccines
Abstract:
... Chickens infected with Salmonella enterica serovars Typhimurium (ST) and Enteritidis (SE) still represent a major source of human food poisoning via consumption of contaminated meat and eggs. Vaccination represents a sustainable approach to control Salmonella in the chicken and the serovar specificity of immunity has the potential to impact on the need for multivalent vaccines. The issue of cross- ...
DOI:
10.1016/j.vetimm.2006.07.011

22. Cytokine and antibody subclass responses in the intestinal lymph of sheep during repeated experimental infections with the nematode parasite Trichostrongylus colubriformis

Author:
Pernthaner, A.; Cole, S.A.; Morrison, L.; Green, R.; Shaw, R.J.; Hein, W.R.
Source:
Veterinary immunology and immunopathology 2006 v.114 no.1-2 pp. 135-148
ISSN:
0165-2427
Subject:
sheep; food animals; lymph; intestines; Trichostrongylus colubriformis; nematode infections; animal parasitic nematodes; interleukins; tumor necrosis factor-alpha; interferons; immunoglobulins; genotype; gene expression; pest resistance; genetic resistance; messenger RNA; immune response; immunity; cytokines
Abstract:
... The expression of interleukin (IL)-4, IL-5, IL-10, IL-13, TNF-α and IFN-γ genes, and parasite-specific IgM, IgG1, IgG2, IgA and total IgE levels, were monitored daily in intestinal lymph of sheep infected repeatedly with the nematode parasite Trichostrongylus colubriformis. Host genotype had a significant influence on IL-13 gene activity, with resistant-line (R) sheep consistently expressing highe ...
DOI:
10.1016/j.vetimm.2006.08.004

23. Differential expression of U2AF35 in the arthritic joint of avian reovirus-infected chicks

Author:
Fan, Y.H.; Lin, C.D.; Chiou, S.H.; Chow, K.C.; Chi, Y.S.; Lee, L.H.; Shien, J.H.; Shieh, H.K.
Source:
Veterinary immunology and immunopathology 2006 v.114 no.1-2 pp. 49-60
ISSN:
0165-2427
Subject:
broiler chickens; chicks; food animals; Reoviridae; arthritis; joints (animal); joint diseases; strains; histopathology; poultry diseases; viral diseases of animals and humans; gene expression; messenger RNA; temporal variation; immunohistochemistry; macrophages; reverse transcriptase polymerase chain reaction; metalloproteinases; nucleic acid hybridization; chondrocytes; immunopathology
Abstract:
... To identify cell types and genes that are differentially expressed during immunopathogenesis of avian reovirus (ARV)-induced viral arthritis (VA), we inoculated arthrotropic strain S1133 of ARV into 1-day-old broilers, and examined tissue histology as well as RNA expression at different days post-inoculation (PI). Using immunohistochemical staining, we detected many CD68 expressing macrophages in ...
DOI:
10.1016/j.vetimm.2006.07.003

24. Differential regulation of porcine β-defensins 1 and 2 upon Salmonella infection in the intestinal epithelial cell line IPI-2I

Author:
Veldhuizen, E.J.A.; Hendriks, H.G.C.J.M.; Hogenkamp, A.; Dijk, A. van; Gaastra, W.; Tooten, P.C.J.; Haagsman, H.P.
Source:
Veterinary immunology and immunopathology 2006 v.114 no.1-2 pp. 94-102
ISSN:
0165-2427
Subject:
swine; intestinal mucosa; epithelial cells; cell lines; Salmonella enteritidis; Salmonella typhimurium; Arcobacter cryaerophilus; animal pathogenic bacteria; gene expression; protein synthesis; salmonellosis; bacterial infections; culture media; cultured cells; cell invasion; messenger RNA; vaccination; viability; gene expression regulation
Abstract:
... Intestinal epithelial cells represent the first line of defence against pathogenic bacteria in the lumen of the gut. Besides acting as a physical barrier, epithelial cells orchestrate the immune response through the production of several innate immune mediator molecules including β-defensins. Here, we establish the porcine intestinal cell line IPI-2I as a new model system to test the regulation of ...
DOI:
10.1016/j.vetimm.2006.07.012

25. Evaluation of cytokines concentration and percentage of survival of rabies virus-infected mice submitted to anti-rabies Vero-cell propagated vaccine and P. acnes

Author:
Megid, J.; Appolinario, C.M.; Mazzini, A.M.; Almeida, M.F.
Source:
Veterinary immunology and immunopathology 2006 v.114 no.1-2 pp. 192-196
ISSN:
0165-2427
Subject:
mice; animal disease models; Rabies virus; cytokines; mortality; rabies; animal diseases; vertebrate viruses; interleukin-6; blood serum; blood chemistry; vaccines; immunomodulators; Propionibacterium acnes; vaccination; signs and symptoms (animals and humans); dosage; pathogenesis
Abstract:
... Previously, survival of rabies infection was shown to correlate with low IL-6 serum concentration in mice subjected to post-exposure treatment with the Fuenzalida Palacios rabies vaccine in conjunction with the immunomodulator Propionibacterium acnes, previously Corynebacterium parvum. Considering the substitution of the Fuenzalida Palacios rabies vaccine by the Vero cell raised anti-rabies vaccin ...
DOI:
10.1016/j.vetimm.2006.07.010

26. Formulation with CpG ODN enhances antibody responses to an equine influenza virus vaccine

Author:
Lopez, A.M.; Hecker, R.; Mutwiri, G.; Van Drunen Littel-van den Hurk, S.; Babiuk, L.A.; Townsend, H.G.G.
Source:
Veterinary immunology and immunopathology 2006 v.114 no.1-2 pp. 103-110
ISSN:
0165-2427
Subject:
horses; Influenza A virus; vertebrate viruses; horse diseases; vaccination; immune response; immunoglobulin G; nucleotide sequences; vaccine adjuvants; inactivated vaccines; antibodies; disease course; disease incidence; signs and symptoms (animals and humans); enzyme-linked immunosorbent assay; immunity
Abstract:
... Previous studies have shown that protection against equine influenza virus (EIV) is partially mediated by virus-specific IgGa and IgGb. In this study we tested whether addition of a CpG ODN formulation to a commercial killed virus vaccine would enhance EIV-specific IgGa and IgGb antibody responses, and improve protection against an experimental EIV challenge. Thirty naïve horses were assigned to ...
DOI:
10.1016/j.vetimm.2006.07.013

27. Generation of canine dendritic cells from peripheral blood monocytes without using purified cytokines

Author:
Wijewardana, V.; Sugiura, K.; Oichi, T.; Fujimoto, M.; Akazawa, T.; Hatoya, S.; Inaba, M.; Ikehara, S.; Jayaweera, T.S.P.; Inaba, T.
Source:
Veterinary immunology and immunopathology 2006 v.114 no.1-2 pp. 37-48
ISSN:
0165-2427
Subject:
dogs; pets; dendritic cells; monocytes; cytokines; cultured cells; culture media; T-lymphocytes; antibodies; major histocompatibility complex; protein synthesis; gene expression; interleukins; interferons; phagocytosis
Abstract:
... Dendritic cells (DCs), which differentiate in vitro from peripheral blood monocytes (PBMOs) or bone marrow precursors, are a promising candidate for immunotherapy against cancer. The dog, which suffers common types of cancers along with humans, make an ideal large animal model for cancer studies. Monocyte-derived DCs in the dog have not been well characterized, however, since the appropriate condi ...
DOI:
10.1016/j.vetimm.2006.07.002

28. Identification of a c-kit exon 8 internal tandem duplication in a feline mast cell tumor case and its favorable response to the tyrosine kinase inhibitor imatinib mesylate

Author:
Isotani, M.; Tamura, K.; Yagihara, H.; Hikosaka, M.; Ono, K.; Washizu, T.; Bonkobara, M.
Source:
Veterinary immunology and immunopathology 2006 v.114 no.1-2 pp. 168-172
ISSN:
0165-2427
Subject:
cats; pets; mast cells; neoplasms; exons; proto-oncogenes; mutation; pathogenesis; sequence analysis; amino acid sequences; kinases; enzyme inhibitors; protein phosphorylation
Abstract:
... The gain-of-function mutations within c-kit, a protooncogene encoding KIT, induce constitutive ligand-independent kinase activation and are important for the pathogenesis of mast cell proliferative disease in humans as well as in dogs. Despite the clinical importance of feline mast cell tumors, no mutation has been shown within the c-kit gene in cats. In the present report, we analyzed the c-kit n ...
DOI:
10.1016/j.vetimm.2006.07.004

29. Lactoferrin expression in the horse endometrium: Relevance in persisting mating-induced endometritis

Author:
Kolm, G.; Klein, D.; Knapp, E.; Watanabe, K.; Walter, I.
Source:
Veterinary immunology and immunopathology 2006 v.114 no.1-2 pp. 159-167
ISSN:
0165-2427
Subject:
mares; endometrium; endometritis; horse diseases; lactoferrin; protein synthesis; antibacterial proteins; immunomodulators; disease resistance; pregnancy; leukocytes; neutrophils; messenger RNA; reverse transcriptase polymerase chain reaction; epithelium; gene expression; temporal variation; inflammation; insemination; estrous cycle
Abstract:
... Lactoferrin (LF) is an estrogen-regulated glycoprotein with well-described antibacterial and immunomodulatory properties. The present study is the first report on LF expression in horse endometrial specimens. Mares chosen for the study were either resistant or susceptible for persisting mating-induced endometritis (PMIE) during the natural ovulatory cycle and in early pregnancy. Our investigations ...
DOI:
10.1016/j.vetimm.2006.08.005

30. Molecular cloning of multiple forms of the ovine B7-2 (CD86) costimulatory molecule

Author:
Terzo, E.A.; Perez de Villarreal, M.; Mick, V.; Munoz, F.; Amorena, B.; Andres, D. de; Perez de la Lastra, J.M.
Source:
Veterinary immunology and immunopathology 2006 v.114 no.1-2 pp. 149-158
ISSN:
0165-2427
Subject:
sheep; food animals; animal proteins; antigens; genes; nucleotide sequences; messenger RNA; alternative splicing; reverse transcriptase polymerase chain reaction; complementary DNA; genetic techniques and protocols; signal peptide; transmembrane proteins; amino acid sequences; gene expression regulation
Abstract:
... To facilitate analysis of the role of costimulatory molecules in the ovine immune system, we cloned and sequenced eight putative alternatively spliced transcripts of the sheep CD86 (B7-2) costimulatory molecule. Using RT-PCR and rapid amplification of cDNA ends (RACE), we cloned the sheep CD86 (B7-2) molecule that encodes eight forms, which differ in the length of the signal peptide, the presence ...
DOI:
10.1016/j.vetimm.2006.08.003

31. Monoclonal antibodies against chicken interleukin-6

Author:
Scott, T.R.; Lillehoj, H.S.
Source:
Veterinary immunology and immunopathology 2006 v.114 no.1-2 pp. 173
ISSN:
0165-2427
Subject:
chickens; food animals; interleukin-6; monoclonal antibodies; recombinant proteins; Western blotting; immunoglobulin G; bioassays; mice; hybridomas; inhibitors; dosage; antigens
Abstract:
... Monoclonal antibodies (mAb) were produced against a recombinant (r) chicken interleukin-6 (IL-6). Eight mAbs produced were tested for isotype; ability to inhibit recombinant forms of chicken (ch), human (h) and murine (m) IL-6; and recognition of rchIL-6 by Western immunoblotting. The mAb isotypes were represented by IgG1 (one), IgG2a (six) and IgG2b (one). In a mouse B9 hybridoma cell bioassay wi ...
Handle:
10113/7397
DOI:
10.1016/j.vetimm.2006.07.009

32. Quantitation of immune response gene expression and cellular localisation of interleukin-1β mRNA in Atlantic salmon, Salmo salar L., affected by amoebic gill disease (AGD)

Author:
Bridle, A.R.; Morrison, R.N.; Cunningham, P.M.C.; Nowak, B.F.
Source:
Veterinary immunology and immunopathology 2006 v.114 no.1-2 pp. 121-134
ISSN:
0165-2427
Subject:
Salmo salar; salmon; immune response; gene expression; quantitative analysis; interleukin-1; messenger RNA; protein synthesis; amebiasis; Amoebida; fish diseases; reverse transcriptase polymerase chain reaction; nucleic acid hybridization; genes; nitric oxide synthase; animal proteins; gills; liver; kidneys; gene expression regulation; infection
Abstract:
... The characterisation of selected immune response genes during amoebic gill disease (AGD) in Atlantic salmon, Salmo salar L., was performed using semi-quantitative RT-PCR, quantitative real-time RT-PCR (qRT-PCR), and in situ hybridisation (ISH). The immune response genes of interest were interleukin-1β (IL-1β), inducible nitric oxide synthase (iNOS), serum amyloid A (SAA), and serum amyloid P-like ...
DOI:
10.1016/j.vetimm.2006.08.002

33. Single-cell analysis divides bovine monocyte-derived dendritic cells into subsets expressing either high or low levels of inducible nitric oxide synthase

Author:
Mirkovitch, J.; Konig, A.; Sauter, K.S.; Brcic, M.; Hope, J.C.; Howard, C.J.; Jungi, T.W.
Source:
Veterinary immunology and immunopathology 2006 v.114 no.1-2 pp. 1-14
ISSN:
0165-2427
Subject:
cattle; monocytes; dendritic cells; nitric oxide synthase; protein synthesis; gene expression; immune system; immunity; messenger RNA; interferons; Listeria monocytogenes; immunocytochemistry; macrophages; antigens; biomarkers; tumor necrosis factors
Abstract:
... Dendritic cells (DC) are important cells at the interface between innate and adaptive immunity. DC have a key role in antigen processing and presentation to T cells. Effector functions of DC related to innate immunity have not been explored extensively. We show that bovine monocyte-derived DC (mDC) express inducible nitric oxide synthase (iNOS) mRNA and protein and produce NO upon triggering with ...
DOI:
10.1016/j.vetimm.2006.06.004

34. The effect of tuberculin testing on the development of cell-mediated immune responses during Mycobacterium bovis infection

Author:
Thom, M.L.; Hope, J.C.; McAulay, M.; Villarreal-Ramos, B.; Coffey, T.J.; Stephens, S.; Vordermeier, H.M.; Howard, C.J.
Source:
Veterinary immunology and immunopathology 2006 v.114 no.1-2 pp. 25-36
ISSN:
0165-2427
Subject:
calves; Mycobacterium bovis; bovine tuberculosis; cell-mediated immunity; tuberculin; diagnostic techniques; serodiagnosis; delayed hypersensitivity; temporal variation; immune response; lymphocyte proliferation; immunoglobulin G; interferons; interleukins; disease course; messenger RNA; protein synthesis; gene expression
Abstract:
... Protection against tuberculosis (TB) is associated with Th1-type cell-mediated immunity (CMI). Whilst the intradermal injection of partially purified derivatives of tuberculin (PPD) represents the classic test assessing the delayed type hypersensitivity (DTH) response used in both humans and cattle for diagnosing TB, it has been suggested that the test may modulate host CMI responses. To investiga ...
DOI:
10.1016/j.vetimm.2006.07.001

35. Canine microglial cells: Stereotypy in immunophenotype and specificity in function

Author:
Stein, V.M.; Baumgartner, W.; Kreienbrock, L.; Zurbriggen, A.; Vandevelde, M.; Tipold, A.
Source:
Veterinary immunology and immunopathology 2006 v.113 no.3-4 pp. 277-287
ISSN:
0165-2427
Subject:
dogs; pets; neuroglia; immune system; central nervous system; temporal variation; animal pathology; flow cytometry; biomarkers; outer membrane proteins; phagocytosis; reactive oxygen species; histopathology; central nervous system diseases; pathogenesis; immunopathology; canine distemper; Canine distemper virus
Abstract:
... Microglial cells represent the endogenous immune system of the central nervous system (CNS). Upon pathological insults they reveal their immunological potential aimed at regaining homeostasis. These reactions have long been believed to follow a uniform and unspecific pattern which is irrespective to the underlying disease entity. Evidence is growing that this view seriously underrates microglial c ...
DOI:
10.1016/j.vetimm.2006.05.011

36. Comparative assessment of Th1 and Th2 cytokines of swamp type buffalo and other bubaline breeds by molecular cloning, sequencing and phylogenetics

Author:
Mingala, C.N.; Odbileg, R.; Konnai, S.; Ohashi, K.; Onuma, M.
Source:
Veterinary immunology and immunopathology 2006 v.113 no.3-4 pp. 348-356
ISSN:
0165-2427
Subject:
buffaloes; genotype; genetic variation; breed differences; cytokines; nucleotide sequences; amino acid sequences; phylogeny; interleukins; interferons; protein secondary structure; messenger RNA; alternative splicing
Abstract:
... Comparative assessment of Th1 and Th2 cytokines of three bubaline breeds namely swamp buffalo, its crossbreed with riverine buffalo (CB), and the improved breed of Bulgarian Murrah buffalo (BMB), was done by molecular cloning, sequencing and phylogenetic analysis. The Th1 cytokines analyzed included IL-2, IL-12p35, IL-12p40, and IFN-γ while Th2 cytokines included IL-4 and IL-10. Both groups showed ...
DOI:
10.1016/j.vetimm.2006.06.005

37. Comparison of binding and effects of Escherichia coli Shiga toxin 1 on bovine and ovine granulocytes

Author:
Menge, C.; Eisenberg, T.; Stamm, I.; Baljer, G.
Source:
Veterinary immunology and immunopathology 2006 v.113 no.3-4 pp. 392-403
ISSN:
0165-2427
Subject:
cattle; sheep; granulocytes; species differences; Escherichia coli; animal pathogenic bacteria; Shiga-like toxin 1; pathogenesis; intestinal mucosa; inflammation; disease reservoirs; zoonoses; cultured cells; blood cells; milk; binding sites; receptors; in vitro studies; apoptosis
Abstract:
... Granulocytes play a pivotal role in the pathogenesis of Shiga toxin (Stx)-producing Escherichia coli (STEC) related diseases in humans. Granulocytes are attracted and activated by Stxs in the enteric mucosa and are believed to thereby contribute to the intestinal inflammation. Mature ruminants, the main reservoir hosts of STEC, do not develop pathological changes that can be attributed to the Stxs ...
DOI:
10.1016/j.vetimm.2006.06.009

38. Escherichia coli Shiga toxin 1 enhances il-4 transcripts in bovine ileal intraepithelial lymphocytes

Author:
Moussay, E.; Stamm, I.; Taubert, A.; Balijer, G.; Menge, C.
Source:
Veterinary immunology and immunopathology 2006 v.113 no.3-4 pp. 367-382
ISSN:
0165-2427
Subject:
cattle; intestinal mucosa; cultured cells; Escherichia coli; animal pathogenic bacteria; Shiga toxin; lymphocytes; immune response; bacterial colonization; reverse transcriptase polymerase chain reaction; cytokines; messenger RNA; gene expression; protein synthesis
Abstract:
... Shiga toxin 1 (Stx1) blocks the activation of bovine peripheral and intraepithelial lymphocytes (IEL), implying that the toxin has the potential to retard the host's immune response during intestinal colonization of cattle with human pathogenic Stx-producing Escherichia coli (STEC). Since Stx1 does not eliminate affected lymphocytes by causing cellular death, we assumed that Stx1 disturbs the inte ...
DOI:
10.1016/j.vetimm.2006.06.007

39. Expression of different classes of immunoglobulin in intraepithelial plasma cells of the Harderian gland of domestic ducks Anas platyrhynchos

Author:
Oliveira, C.A.; Telles, L.F.; Oliveira, A.G.; Kalapothakis, E.; Goncalves-Dornelas, H.; Mahecha, G.A.B.
Source:
Veterinary immunology and immunopathology 2006 v.113 no.3-4 pp. 257-266
ISSN:
0165-2427
Subject:
ducks; animal tissues; epithelial cells; plasma cells; immunoglobulin M; immunoglobulin A; immunoglobulins; protein synthesis; immune system; immunoglobulin Y
Abstract:
... The Harderian gland of chickens contains numerous plasma cells and is considered as a peripheral lymphoid organ. Data about this gland in other avian species are scarce or inexistent. Considering that ducks show some unique characteristics regarding the immune system, which are important in evolutionary context, and that unusual location of plasma cells into the epithelium was recently described i ...
DOI:
10.1016/j.vetimm.2006.05.008

40. Expression of rabbit interleukin-4 and characterization of its biologic activity on T and B-cells

Author:
Leader, B.T.; Van Voorhis, W.C.; Lukehart, S.A.
Source:
Veterinary immunology and immunopathology 2006 v.113 no.3-4 pp. 421-427
ISSN:
0165-2427
Subject:
rabbits; interleukin-4; T-lymphocytes; B-lymphocytes; recombinant proteins; complementary DNA; nucleotide sequences; genetic vectors; molecular weight; immunoblotting; lymphocyte proliferation; major histocompatibility complex
Abstract:
... The purpose of the current study was to express recombinant rabbit IL-4 (rRbIL-4) and to characterize its biological activity. The cDNA of RbIL-4 was cloned into an insect cell expression vector that allowed for constitutive expression in Sf9 cells and incorporated a 6-histidine tag on the recombinant protein for purification. The purified protein corresponded to the predicted size of rRbIL-4 and ...
DOI:
10.1016/j.vetimm.2006.06.001