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Veterinary immunology and immunopathology
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Publication year rev
7998-2002
Remove constraint Publication year rev: 7998-2002
Subject
Western blotting
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- Author:
- Rawson, Renee R.; Belov, Katherine; Gidley-Baird, A. Angus; Cooper, Desmond W.
- Source:
- Veterinary immunology and immunopathology 2002 v.88 no.1-2 pp. 89-95
- ISSN:
- 0165-2427
- Subject:
- Macropus eugenii; Macropus giganteus; Phascolarctos cinereus; Trichosurus vulpecula; Western blotting; antiserum; blood serum; cross reaction; enzyme-linked immunosorbent assay; immunoglobulin A; immunoglobulin G; mucosal immunity; possums; recombinant proteins
- Abstract:
- ... One of the limiting factors in understanding immune responses in marsupials is the scarcity of marsupial specific immunological reagents. This paper describes the characterisation of an antiserum raised against a recombinant protein of the constant region of the heavy chain of IgA (Cα) of the common brushtail possum (Trichosurus vulpecula). The availability of a marsupial specific anti-IgA provide ...
- DOI:
- 10.1016/S0165-2427(02)00126-5
- https://doi.org/10.1016/S0165-2427(02)00126-5
- Author:
- Wonderling, Ramani; Powell, Tim; Baldwin, Susan; Morales,Tony; Snyder, Scott; Keiser, Kathy; Hunter, Shirley; Best, Elaine; McDermott, Martin J.; Milhausen, Michael
- Source:
- Veterinary immunology and immunopathology 2002 v.89 no.1-2 pp. 13-27
- ISSN:
- 0165-2427
- Subject:
- sequence analysis; interferon-alpha; antiviral properties; Komagataella pastoris; complementary DNA; cats; cell culture; glycosylation; humans; recombinant proteins; yeasts; Western blotting; amino acid sequences; amino acids; clones; genes; viruses; immune response
- Abstract:
- ... Type I interferons (IFN) are important mediators of the host defense against viral infections in mammals. In humans multiple subtypes of IFN-α exist, most of which possess antiviral activity. Little is known about the type I IFN genes in cats and the role they may play in feline immunological responses to viruses. We have isolated cDNAs encoding five feline IFN-α (feIFN) subtypes that share from 9 ...
- DOI:
- 10.1016/S0165-2427(02)00188-5
- https://doi.org/10.1016/S0165-2427(02)00188-5
- Author:
- Min, Wongi; Lillehoj, Hyun S.; Li, Guangxing; Sohn, Eun J.; Miyamoto, Tadashi
- Source:
- Veterinary immunology and immunopathology 2002 v.88 no.1-2 pp. 49-56
- ISSN:
- 0165-2427
- Subject:
- T-lymphocytes; Western blotting; cell growth; chickens; enzyme-linked immunosorbent assay; genes; interleukin-15; interleukin-2; monoclonal antibodies; polypeptides
- Abstract:
- ... The chicken IL-15 gene was recently cloned and shown to encode a polypeptide with T cell growth factor activity similar to IL-2. To further characterize the chemical and biological properties of chicken IL-15, we generated a panel of monoclonal antibodies against bacterially expressed protein and characterized their binding specificities. All antibodies were reactive by ELISA with recombinant IL-1 ...
- DOI:
- 10.1016/S0165-2427(02)00123-X
- https://doi.org/10.1016/S0165-2427(02)00123-X
- Author:
- Uhl, E.W.; Heaton-Jones, T.G.; Pu, R.; Yamamoto, J.K.
- Source:
- Veterinary immunology and immunopathology 2002 v.90 no.3-4 pp. 113-132
- ISSN:
- 0165-2427
- Subject:
- Feline immunodeficiency virus; Human immunodeficiency virus; USDA; Western blotting; acquired immunodeficiency syndrome; cats; cross reaction; diagnostic techniques; human diseases; humans; industry; medicine; models; vaccine development; vaccines; veterinary medicine
- Abstract:
- ... Feline immunodeficiency virus (FIV) is a natural infection of domestic cats that results in acquired immunodeficiency syndrome resembling human immunodeficiency virus (HIV) infection in humans. The worldwide prevalence of FIV infection in domestic cats has been reported to range from 1 to 28%. Hence, an effective FIV vaccine will have an important impact on veterinary medicine in addition to being ...
- DOI:
- 10.1016/S0165-2427(02)00227-1
- PubMed:
- 12459160
- PubMed Central:
- PMC7119750
- https://doi.org/10.1016/S0165-2427(02)00227-1
- Author:
- Pedersen, L.G.; Castelruiz, Y.; Jacobsen, S.; Aasted, B.
- Source:
- Veterinary immunology and immunopathology 2002 v.88 no.3-4 pp. 111-122
- ISSN:
- 0165-2427
- Subject:
- Western blotting; cattle; dogs; flow cytometry; goats; granulocyte-macrophage colony-stimulating factor; horses; humans; interferon-gamma; interleukin-2; interleukin-4; interleukin-6; interleukin-8; mink; monoclonal antibodies; mononuclear leukocytes; rabbits; sheep; swine; tumor necrosis factor-alpha
- Abstract:
- ... Eleven monoclonal antibodies specific for ovine, bovine and human cytokines were investigated by flow cytometry for cross-reactivities with cytokines produced by peripheral blood mononuclear cells (PBMCs) from sheep, cattle, goat, swine, horse, dog, mink, rabbit and human. Four antibodies specific for IL-4, IL-8, IFN-γ and TNF-α cross-reacted with cytokines from a majority of the species investiga ...
- DOI:
- 10.1016/S0165-2427(02)00139-3
- https://doi.org/10.1016/S0165-2427(02)00139-3
- Author:
- Patarroyo, J.H.; Portela, R.W.; Castro, R.O. De; Pimentel, J. Couto; Guzman, F.; Patarroyo, M.E.; Vargas, M.I.; Prates, A.A.; Mendes, M.A. Dias
- Source:
- Veterinary immunology and immunopathology 2002 v.88 no.3-4 pp. 163-172
- ISSN:
- 0165-2427
- Subject:
- Rhipicephalus microplus; Western blotting; antibodies; cattle; digestive system; females; glycoproteins; immune response; immunization; immunoglobulin G; oviposition; saponins; synthetic peptides; ticks
- Abstract:
- ... Three synthetic peptides (SBm4912, SBm7462 and SBm19733), derived from the Bm86 glycoprotein from Boophilus microplus gut, were constructed and used to immunize cattle from a tick-free area. The immunized animals received three subcutaneous doses of the peptides, with saponin as adjuvant, at 30-day intervals. The immune response was evaluated by IgG elicited against the peptides by the detection o ...
- DOI:
- 10.1016/S0165-2427(02)00154-X
- https://doi.org/10.1016/S0165-2427(02)00154-X
- Author:
- McNeil, Heather J.; Shewen, Patricia E.; Lo, Reggie Y.C.; Conlon, Jennifer A.; Miller, Michael W.
- Source:
- Veterinary immunology and immunopathology 2002 v.90 no.1-2 pp. 107-110
- ISSN:
- 0165-2427
- Subject:
- Mannheimia haemolytica serotype 1; Pasteurella trehalosi; Western blotting; disease control; fibrinogen; pneumonia; serotypes; virulence
- Abstract:
- ... Fibrinogen-binding proteins were found in the culture supernatants of Mannheimia haemolytica serotype 1 (ATCC 43270) and Pasteurella trehalosi serotype 10 (ECO-100). Sheep fibrinogen was biotinylated and shown to bind to proteins in the culture supernatants by modified western blot. Fibrinogen-binding proteins in the culture supernatant may be important virulence factors leading to the characteris ...
- DOI:
- 10.1016/S0165-2427(02)00222-2
- https://doi.org/10.1016/S0165-2427(02)00222-2
- Author:
- Werling, D.; Koss, M.; Howard, C.J.; Taylor, G.; Langhans, W.; Hope, J.C.
- Source:
- Veterinary immunology and immunopathology 2002 v.87 no.3-4 pp. 225-233
- ISSN:
- 0165-2427
- Subject:
- chemokine CCL5; coculture; Bovine orthopneumovirus; cattle; dendritic cells; inflammation; reverse transcriptase polymerase chain reaction; Western blotting; viruses; CD4-positive T-lymphocytes; cell proliferation
- Abstract:
- ... Respiratory syncytial virus (RSV) has been reported to induce the production of chemokines in the airway epithelia. Dendritic cells (DC) are the most potent antigen-presenting cells. They are located throughout the body and release chemokines in response to inflammation and infection. We have investigated the chemokine profile of bovine DC in response to exposure to bovine RSV (BRSV). Transcripts ...
- DOI:
- 10.1016/S0165-2427(02)00086-7
- https://doi.org/10.1016/S0165-2427(02)00086-7