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You searched for: Journal name Veterinary immunology and immunopathology Remove constraint Journal name: Veterinary immunology and immunopathology Publication year rev 7998-2002 Remove constraint Publication year rev: 7998-2002 Text availability Citation in PubAg Remove constraint Text availability: Citation in PubAg
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1. A novel transcutaneous plasmid–dimethylsulfoxide delivery technique for avian nucleic acid immunization

Author:
Heckert, R.A.; Elankumaran,S.; Oshop, G.L.; Vakharia, V.N.
Source:
Veterinary immunology and immunopathology 2002 v.89 no.1-2 pp. 67-81
ISSN:
0165-2427
Subject:
chickens; green fluorescent protein; immunoglobulin M; messenger RNA; blood serum; vaccination; dimethyl sulfoxide; macrophages; mucosal immunity; plasmids; immunoglobulin A; Avian orthoavulavirus 1; bone marrow; tears; transfection; Infectious bursal disease virus; immune response; recombinant vaccines; reporter genes; virulence
Abstract:
... In this report, we show that dimethylsulfoxide (DMSO) enhances liposome-mediated transfection of nucleic acid in chicken macrophage cells and that this could be exploited for the transcutaneous delivery of naked DNA through the intact skin of chickens. We found that DMSO enhanced transfection efficiencies of lipofectamine and polyethyleneimine in HD-11 chicken macrophage cells. Based on this princ ...
DOI:
10.1016/S0165-2427(02)00186-1

2. Activation of bovine peripheral blood γδ T cells for cell division and IFN-γ production

Author:
Baldwin, C.L.; Sathiyaseelan, T.; Naiman, B.; White, A.M.; Brown, R.; Blumerman, S.; Rogers, A.; Black, S.J.
Source:
Veterinary immunology and immunopathology 2002 v.87 no.3-4 pp. 251-259
ISSN:
0165-2427
Subject:
CD4-positive T-lymphocytes; cattle; cell division; concanavalin A; flow cytometry; interferon-gamma; interleukin-12; monoclonal antibodies; monocytes; vaccines
Abstract:
... Bovine peripheral blood γδ T cells have been evaluated for effector function (IFN-γ production) and clonal expansion in a variety of systems including following activation by mitogens, IL-12, and stimulation, through the T cell receptor (TCR) with anti-CD3 monoclonal antibody (mAb), a cell-bound molecule and a soluble antigenic extract. To evaluate cell division, carboxyfluorescein succinimidyl es ...
DOI:
10.1016/S0165-2427(02)00091-0

3. Altered expression of cellular genes in neutrophils of periparturient dairy cows

Author:
Madsen, Sally A.; Weber, Patty S.D.; Burton, Jeanne L.
Source:
Veterinary immunology and immunopathology 2002 v.86 no.3-4 pp. 159-175
ISSN:
0165-2427
Subject:
DNA-binding proteins; Northern blotting; animal health; blood serum; complementary DNA; cytochrome b; dairy cows; estradiol; gene expression; gene expression regulation; genes; immune response; mastitis; messenger RNA; metabolism; neutrophils; nucleic acid hybridization; nucleotide sequences; parturition; progesterone; radioimmunoassays; reverse transcriptase polymerase chain reaction; screening; sequence homology; steroid hormones; tricarboxylic acid cycle
Abstract:
... The periparturient dairy cow undergoes a plethora of physiological changes, including changes in the immune system that lead to profound effects on animal health. Of the immune cells affected at parturition, the neutrophil has been of particular interest due to its primary role in innate immune defense against mastitis. Immune functions of bovine neutrophils are known to be depressed around partur ...
DOI:
10.1016/S0165-2427(02)00020-X

4. Antibodies as effectors

Author:
Corbeil, L.B.
Source:
Veterinary immunology and immunopathology 2002 v.87 no.3-4 pp. 169-175
ISSN:
0165-2427
Subject:
antibodies; antigens; autoimmune diseases; cattle; pathogens
Abstract:
... Antibodies are critical in protection against extracellular microbial pathogens. Although antibodies also play a role in transplant/tumor rejection and in autoimmune disease, this paper focuses on defense against bovine infections. Effector mechanisms of different bovine isotypes, subisotypes and allotypes are discussed. The importance of antigen specificity is also stressed. ...
DOI:
10.1016/S0165-2427(02)00048-X

5. B cell development in gut associated lymphoid tissues

Author:
Ratcliffe, Michael J.H.
Source:
Veterinary immunology and immunopathology 2002 v.87 no.3-4 pp. 337-340
ISSN:
0165-2427
Subject:
B-lymphocytes; bone marrow; bursa of Fabricius; chickens; embryogenesis; ileum; immunoglobulins; rodents; sheep; skeletal development
Abstract:
... B lymphocyte development can occur in a variety of anatomical sites. While typically considered to be a process that occurs in the bone marrow throughout life, it is becoming clear that gut associates sites of B cell development are critically important in many species of veterinary importance. Among these sites, the bursa of Fabricius in chickens and the ileal Peyer’s patches of sheep are among t ...
DOI:
10.1016/S0165-2427(02)00061-2

6. Bluetongue virus-induced activation of primary bovine lung microvascular endothelial cells

Author:
DeMaula, Christopher D.; Leutenegger, Christian M.; Jutila, Mark A.; MacLachlan, N. James
Source:
Veterinary immunology and immunopathology 2002 v.86 no.3-4 pp. 147-157
ISSN:
0165-2427
Subject:
Bluetongue virus; apoptosis; blood coagulation; bluetongue; cattle; endothelial cells; genes; inducible nitric oxide synthase; interleukin-1; interleukin-6; interleukin-8; messenger RNA; pathogenesis; prostaglandin synthase; quantitative polymerase chain reaction; sheep
Abstract:
... Bluetongue is an insect-transmitted viral disease of sheep and some species of wild ruminants. Infection of lung microvascular endothelial cells (ECs) is central to the pathogenesis of bluetongue virus (BTV) infection of ruminants, but it is uncertain as to why cattle are resistant to BTV-induced microvascular injury and bluetongue disease. Thus, in order to better understand the pathogenesis of B ...
DOI:
10.1016/S0165-2427(02)00012-0

7. Changes in the leucocyte subpopulations of the palatine tonsillar crypt epithelium of pigs in response to Streptococcus suis type 2 infection

Author:
Salles, M.W.S.; Perez-Casal, J.; Willson, P.; Middleton, D.M.
Source:
Veterinary immunology and immunopathology 2002 v.87 no.1-2 pp. 51-63
ISSN:
0165-2427
Subject:
Streptococcus suis; T-lymphocytes; arthritis; epithelial cells; epithelium; innate immunity; macrophages; major histocompatibility complex; meningitis; monoclonal antibodies; neutrophils; pathogenesis; pathogens; septicemia; swine; tonsils; vaccination
Abstract:
... The tonsils are portal of entry and a site of multiplication and persistence for a variety of pathogens, including Streptococcus suis (S. suis), which is a common cause of meningitis, septicemia and arthritis in pigs. Understanding the early changes that occur in the first barrier of the tonsil, i.e. the crypt epithelium, in response to S. suis infection is critical in clarifying the pathogenesis ...
DOI:
10.1016/S0165-2427(02)00040-5

8. Characterisation of a resource population of pigs screened for resistance to salmonellosis

Author:
Van Diemen, P.M.; Kreukniet, M.B.; Galina, L.; Bumstead, N.; Wallis, T.S.
Source:
Veterinary immunology and immunopathology 2002 v.88 no.3-4 pp. 183-196
ISSN:
0165-2427
Subject:
Salmonella enterica; antibodies; boars; breeding; genes; liver; lymphocytes; neutrophils; piglets; progeny; salmonellosis; spleen; survival rate
Abstract:
... The degree of resistance to Salmonella choleraesuis infection in a reference family purposely bred to map resistance genes was assessed. Aspects of the innate and specific immune system were studied to find a parameter that might predict the resistance of pigs to salmonellosis. The family was bred from commercial full-sister pairs of F1-gilts and four boars. One boar (G398) was identified as breed ...
DOI:
10.1016/S0165-2427(02)00165-4

9. Characterisation of antisera to recombinant IgA of the common brushtail possum (Trichosurus vulpecula)

Author:
Rawson, Renee R.; Belov, Katherine; Gidley-Baird, A. Angus; Cooper, Desmond W.
Source:
Veterinary immunology and immunopathology 2002 v.88 no.1-2 pp. 89-95
ISSN:
0165-2427
Subject:
Macropus eugenii; Macropus giganteus; Phascolarctos cinereus; Trichosurus vulpecula; Western blotting; antiserum; blood serum; cross reaction; enzyme-linked immunosorbent assay; immunoglobulin A; immunoglobulin G; mucosal immunity; possums; recombinant proteins
Abstract:
... One of the limiting factors in understanding immune responses in marsupials is the scarcity of marsupial specific immunological reagents. This paper describes the characterisation of an antiserum raised against a recombinant protein of the constant region of the heavy chain of IgA (Cα) of the common brushtail possum (Trichosurus vulpecula). The availability of a marsupial specific anti-IgA provide ...
DOI:
10.1016/S0165-2427(02)00126-5

10. Characterization and functional analysis of skin-derived dendritic cells from swine without a requirement for in vitro propagation

Author:
Bautista, Elida M.; Gregg, Douglas; Golde, William T.
Source:
Veterinary immunology and immunopathology 2002 v.88 no.3-4 pp. 131-148
ISSN:
0165-2427
Subject:
antigens; dendritic cells; endocytosis; explants; functional properties; immune response; lymphocytes; macrophages; models; monocytes; polystyrenes; swine
Abstract:
... A low-density cell population was isolated from skin explants of pigs and characterized as a highly enriched dendritic cell (DC) population based on phenotypical and functional properties. The skin-derived DCs were identified by their characteristic ultrastructural properties as well as by consistent co-expression of the CD1 and SWC3a antigens that clearly differentiate them from other porcine leu ...
DOI:
10.1016/S0165-2427(02)00152-6

11. Cloning, expression, purification, and biological activity of five feline type I interferons

Author:
Wonderling, Ramani; Powell, Tim; Baldwin, Susan; Morales,Tony; Snyder, Scott; Keiser, Kathy; Hunter, Shirley; Best, Elaine; McDermott, Martin J.; Milhausen, Michael
Source:
Veterinary immunology and immunopathology 2002 v.89 no.1-2 pp. 13-27
ISSN:
0165-2427
Subject:
sequence analysis; interferon-alpha; antiviral properties; Komagataella pastoris; complementary DNA; cats; cell culture; glycosylation; humans; recombinant proteins; yeasts; Western blotting; amino acid sequences; amino acids; clones; genes; viruses; immune response
Abstract:
... Type I interferons (IFN) are important mediators of the host defense against viral infections in mammals. In humans multiple subtypes of IFN-α exist, most of which possess antiviral activity. Little is known about the type I IFN genes in cats and the role they may play in feline immunological responses to viruses. We have isolated cDNAs encoding five feline IFN-α (feIFN) subtypes that share from 9 ...
DOI:
10.1016/S0165-2427(02)00188-5

12. Co-stimulation and modulation of the ensuing immune response

Author:
Howard, C.J.; Hope, J.C.; Stephens, S.A.; Gliddon, D.R.; Brooke, G.P.
Source:
Veterinary immunology and immunopathology 2002 v.87 no.3-4 pp. 123-130
ISSN:
0165-2427
Subject:
CD8-positive T-lymphocytes; antigen presentation; cattle; chemokines; crosslinking; dendritic cells; enzyme activity; humans; immune response; interleukin-10; interleukin-12; interleukin-1alpha; long term effects; lymph; models; rodents; secretion; tumor necrosis factor-alpha; tyrosine
Abstract:
... As a consequence of the central role of dendritic cells (DC) in stimulating primary immune responses any bias in the response introduced by the DC has the potential for having a long-term effect on immunity. Examination and analysis of ruminant afferent lymph dendritic cells derived by cannulation allows studies on the properties of ex vivo DC that is not possible in humans and rodents and informa ...
DOI:
10.1016/S0165-2427(02)00043-0

13. Development and characterization of monoclonal antibodies to chicken interleukin-15

Author:
Min, Wongi; Lillehoj, Hyun S.; Li, Guangxing; Sohn, Eun J.; Miyamoto, Tadashi
Source:
Veterinary immunology and immunopathology 2002 v.88 no.1-2 pp. 49-56
ISSN:
0165-2427
Subject:
T-lymphocytes; Western blotting; cell growth; chickens; enzyme-linked immunosorbent assay; genes; interleukin-15; interleukin-2; monoclonal antibodies; polypeptides
Abstract:
... The chicken IL-15 gene was recently cloned and shown to encode a polypeptide with T cell growth factor activity similar to IL-2. To further characterize the chemical and biological properties of chicken IL-15, we generated a panel of monoclonal antibodies against bacterially expressed protein and characterized their binding specificities. All antibodies were reactive by ELISA with recombinant IL-1 ...
DOI:
10.1016/S0165-2427(02)00123-X

14. Development of a baculovirus expression system for soluble porcine tumor necrosis factor receptor type I and soluble porcine tumor necrosis factor receptor type I-IgG fusion protein

Author:
Mwangi, Simon Musyoka; Stabel, Thomas J.; Kehrli, Marcus E. Jr.
Source:
Veterinary immunology and immunopathology 2002 v.86 no.3-4 pp. 251-254
ISSN:
0165-2427
Subject:
Salmonella enterica; affinity chromatography; antibodies; cell culture; culture media; cytotoxicity; fibrosarcoma; immunoglobulin G; in vitro studies; inflammation; insects; mice; salmonellosis; sepsis (infection); swine; tumor necrosis factor-alpha
Abstract:
... Tumor necrosis factor-α (TNF-α) is a key mediator of inflammatory responses and gram-negative bacterial sepsis, but the role that it plays during Salmonella enterica species bacterial infections in swine has not yet been elucidated. To facilitate studies on the role of TNF-α on the pathology associated with Salmonella infections in pigs, recombinant soluble porcine TNF receptor type I (rspTNF-RI) ...
DOI:
10.1016/S0165-2427(02)00034-X

15. Does cortisol bias cytokine production in cultured porcine splenocytes to a Th2 phenotype

Author:
Skjolaas, K.A.; Minton, J.E.
Source:
Veterinary immunology and immunopathology 2002 v.87 no.3-4 pp. 451-458
ISSN:
0165-2427
Subject:
T-lymphocytes; blood serum; concanavalin A; cortisol; dexamethasone; interferon-gamma; interleukin-10; interleukin-2; macrophage migration inhibitory factors; messenger RNA; mineralocorticoid receptors; phenotype; splenocytes; swine; synthetic glucocorticoids
Abstract:
... Glucocorticoids are reported to bias the production of cytokines from a type 1 to a type 2 phenotype. However, this dogma has been advanced largely from studies utilizing potent glucocorticoid analogs, particularly dexamethasone (DEX). Although studies utilizing DEX certainly have clinical and pharmacological relevance, DEX is probably not the best glucocorticoid for studies designed to evaluate t ...
DOI:
10.1016/S0165-2427(02)00073-9

16. Early development of immune system in pigs

Author:
Sinkora, J.; Rehakova, Z.; Sinkora, M.; Cukrowska, B.; Tlaskalova-Hogenova, H.
Source:
Veterinary immunology and immunopathology 2002 v.87 no.3-4 pp. 301-306
ISSN:
0165-2427
Subject:
T-lymphocytes; antibodies; bacteria; bone marrow; early development; flow cytometry; germ-free animals; immunoglobulin A; immunoglobulin G; liver; miniature swine; phenotype; piglets; pregnancy; thymocytes; viruses; yolk sac
Abstract:
... Prenatal and early postnatal immune system development has been studied in minipigs. First leukocytes were observed in the yolk sac and fetal liver (FL) on the 17th day of gestation, the majority of them being SWC3+. The colonization of the thymus (TH) with leukocytes was observed 21 days later. Two waves of fetal TH colonization with pro-T cells were deduced from the frequency of thymocyte subset ...
DOI:
10.1016/S0165-2427(02)00056-9

17. Effects of phenoxyethanol on the innate immune system of gilthead seabream (Sparus aurata L.) exposed to crowding stress

Author:
Ortuño, Jesús; Esteban, M. Angeles; Meseguer, J.
Source:
Veterinary immunology and immunopathology 2002 v.89 no.1-2 pp. 29-36
ISSN:
0165-2427
Subject:
Sparus aurata; anesthesia; aquaculture; blood glucose; blood serum; complement; cortisol; fish; immune system; phagocytosis; sedatives; stress response
Abstract:
... Phenoxyethanol is routinely used in seabream aquaculture to minimise fish stress response despite the secondary negative effects which have been observed. In this study, two different doses (60 and 200 μl/l) of phenoxyethanol, sedative and narcotic, were tested for their ability to reduce the stress caused in gilthead seabream (Sparus aurata L.) by crowding. Blood glucose and serum cortisol concen ...
DOI:
10.1016/S0165-2427(02)00183-6

18. Effects of prenatal stress on cellular and humoral immune responses in neonatal pigs

Author:
Tuchscherer, M.; Kanitz, E.; Otten, W.; Tuchscherer, A.
Source:
Veterinary immunology and immunopathology 2002 v.86 no.3-4 pp. 195-203
ISSN:
0165-2427
Subject:
B-lymphocytes; Phytolacca americana; T-lymphocytes; blood serum; concanavalin A; corticotropin; cytotoxicity; disease resistance; humoral immunity; immune response; immunoglobulin G; lymphocyte proliferation; morbidity; mortality; ontogeny; piglets; pregnancy; progeny; sows; suckling
Abstract:
... In the present study, we investigated the effects of a daily 5 min restraint stress of pregnant sows in the last five gestational weeks on the development and reactivity of the immune system of the offspring. Maternal stress resulted in significant decreased serum immunoglobulin G (IgG) concentrations in suckling piglets at 1 and 3 days of age. Furthermore, the stress treatment of the sows had an ...
DOI:
10.1016/S0165-2427(02)00035-1

19. Failure to demonstrate involvement of antibodies to Acinetobacter calcoaceticus in transmissible spongiform encephalopathies of animals

Author:
Nielsen, K.; Widdison, J.; Balachandran, A.; Stevenson, D.; Algire, J.
Source:
Veterinary immunology and immunopathology 2002 v.89 no.3-4 pp. 197-205
ISSN:
0165-2427
Subject:
Acinetobacter calcoaceticus; antibodies; antigens; blood serum; brain; cattle; elks; feeds; lipopolysaccharides; myelin basic protein; prion diseases; sheep; soil microorganisms
Abstract:
... Acinetobacter calcoaceticus, a soil microbe, contains molecular sequences which resemble those found in neurofilaments of the brain tissue. It was hypothesized that if cattle ingest large amounts of feedstuff containing A. calcoaceticus, they may develop an autoimmune reaction, with consequences of pathological changes associated with transmissible spongiform encephalopathies (TSEs). The hypothesi ...
DOI:
10.1016/S0165-2427(02)00209-X

20. Genomic sequencing of the bovine T cell receptor beta locus

Author:
Conrad, M.L.; Pettman, R.; Whitehead, J.; McKinnel, L.; Davis, S.K.; Koop, B.F.
Source:
Veterinary immunology and immunopathology 2002 v.87 no.3-4 pp. 439-441
ISSN:
0165-2427
Subject:
T-lymphocytes; cattle; evolution; genes; genomic libraries; humans; loci; mice; sequence analysis
Abstract:
... The T cell, which plays an integral role in the coordination of the immune system, has a heterodimer receptor (TCR) that can exist in one of the two forms: alpha/beta or gamma/delta. Cells displaying the gamma/delta TCR comprise less than 5% of T cell populations in humans and mice. In the bovine system, however, gamma/delta populations can reach as high as 60%. Differences in T cell populations m ...
DOI:
10.1016/S0165-2427(02)00093-4