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You searched for: Journal name Veterinary immunology and immunopathology Remove constraint Journal name: Veterinary immunology and immunopathology Publication year rev 7998-2002 Remove constraint Publication year rev: 7998-2002
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1. A novel model for equine recurrent airway obstruction

Author:
Bowles, K.S.; Beadle, R.E.; Mouch, S.; Pourciau, S.S.; Littlefield-Chabaud, M.A.; Le Blanc, C.; Mistric, L.; Fermaglich, D.; Horohov, D.W.
Source:
Veterinary immunology and immunopathology 2002 v.87 no.3-4 pp. 385-389
ISSN:
0165-2427
Subject:
aerosols; antigens; horses; hypersensitivity; interleukin-13; interleukin-4; lymphocytes; messenger RNA; models; neutrophils; pastures; polystyrenes; respiratory tract diseases; summer
Abstract:
... Equine recurrent airway obstruction (RAO; a term combining both chronic obstructive pulmonary disease (COPD) and summer pasture associated obstructive pulmonary disease (SPAOPD)) is one of the most common equine respiratory diseases with up to 50% of horses affected worldwide. The etiopathogenesis of RAO is unknown although pulmonary hypersensitivity to inhaled mold antigens may be involved. Recen ...
DOI:
10.1016/S0165-2427(02)00081-8

2. A novel transcutaneous plasmid–dimethylsulfoxide delivery technique for avian nucleic acid immunization

Author:
Heckert, R.A.; Elankumaran,S.; Oshop, G.L.; Vakharia, V.N.
Source:
Veterinary immunology and immunopathology 2002 v.89 no.1-2 pp. 67-81
ISSN:
0165-2427
Subject:
chickens; green fluorescent protein; immunoglobulin M; messenger RNA; blood serum; vaccination; dimethyl sulfoxide; macrophages; mucosal immunity; plasmids; immunoglobulin A; Avian orthoavulavirus 1; bone marrow; tears; transfection; Infectious bursal disease virus; immune response; recombinant vaccines; reporter genes; virulence
Abstract:
... In this report, we show that dimethylsulfoxide (DMSO) enhances liposome-mediated transfection of nucleic acid in chicken macrophage cells and that this could be exploited for the transcutaneous delivery of naked DNA through the intact skin of chickens. We found that DMSO enhanced transfection efficiencies of lipofectamine and polyethyleneimine in HD-11 chicken macrophage cells. Based on this princ ...
DOI:
10.1016/S0165-2427(02)00186-1

3. A rapid and simple method for the separation of pure lymphocytes from horse blood

Author:
Zizzadoro, Claudia; Belloli, Chiara; Badino, Paola; Ormas, Paolo
Source:
Veterinary immunology and immunopathology 2002 v.89 no.1-2 pp. 99-104
ISSN:
0165-2427
Subject:
anticoagulants; centrifugation; erythrocytes; horses; lymphocytes; neutrophils; sodium citrate; temperature; viability
Abstract:
... A method for the separation of pure and viable lymphocytes and granulocytes from the same blood sample in horses was reported. By centrifuging equine heparinized blood at 100×g for 10 min at room temperature (r.t.), the resulting supernatant plasma was an almost pure (97.71±0.30%; n=15) suspension of highly viable (98.72±0.28%) lymphocytes. When sodium citrate was used as an anticoagulant, lymphoc ...
DOI:
10.1016/S0165-2427(02)00185-X

4. A sub-population of circulating porcine γδ T cells can act as professional antigen presenting cells

Author:
Takamatsu, H.H.; Denyer, M.S.; Wileman,T.E.
Source:
Veterinary immunology and immunopathology 2002 v.87 no.3-4 pp. 223-224
ISSN:
0165-2427
Subject:
CD4-positive T-lymphocytes; antigen presentation; antigen-presenting cells; chloroquine; monoclonal antibodies; ovalbumin; pretreatment; surface antigens; swine
Abstract:
... A sub-population of circulating porcine γδ T cells express cell surface antigens associated with antigen presenting cells (APCs), and are able to take up soluble antigen very effectively. Functional antigen presentation by γδ T cells to memory helper T cells was studied by inbred pig lymphocytes immunised with ovalbumin (OVA). After removing all conventional APCs from the peripheral blood of immun ...
DOI:
10.1016/S0165-2427(02)00083-1

5. Activation of bovine peripheral blood γδ T cells for cell division and IFN-γ production

Author:
Baldwin, C.L.; Sathiyaseelan, T.; Naiman, B.; White, A.M.; Brown, R.; Blumerman, S.; Rogers, A.; Black, S.J.
Source:
Veterinary immunology and immunopathology 2002 v.87 no.3-4 pp. 251-259
ISSN:
0165-2427
Subject:
CD4-positive T-lymphocytes; cattle; cell division; concanavalin A; flow cytometry; interferon-gamma; interleukin-12; monoclonal antibodies; monocytes; vaccines
Abstract:
... Bovine peripheral blood γδ T cells have been evaluated for effector function (IFN-γ production) and clonal expansion in a variety of systems including following activation by mitogens, IL-12, and stimulation, through the T cell receptor (TCR) with anti-CD3 monoclonal antibody (mAb), a cell-bound molecule and a soluble antigenic extract. To evaluate cell division, carboxyfluorescein succinimidyl es ...
DOI:
10.1016/S0165-2427(02)00091-0

6. Allergic pulmonary and ocular tissue responses in the absence of serum IgE antibodies (IgE) in an allergic dog model

Author:
Zemann, B.; Griot-Wenk, M.; Marti, E.; Mayer, P.; Eder, C.; Nefzger, M.; Schneider, H.; Weck, A. de; Liehl, E.
Source:
Veterinary immunology and immunopathology 2002 v.87 no.3-4 pp. 373-378
ISSN:
0165-2427
Subject:
Beagle; antibodies; blood serum; compliance; detection limit; dogs; eosinophilia; hypersensitivity; immunoglobulin E; lungs; models; ovalbumin
Abstract:
... Allergen-specific serum IgE may be insensitive as a marker for IgE-mediated reactions at the mucosal level. Five of six atopic beagle dogs developed high ovalbumin (OVA)-specific serum IgE levels after sensitization. This study aimed to show that these dogs still express allergen-specific IgE at the pulmonary and ocular mucosal levels and in the skin even when corresponding serum IgE was below the ...
DOI:
10.1016/S0165-2427(02)00071-5

7. Altered expression of cellular genes in neutrophils of periparturient dairy cows

Author:
Madsen, Sally A.; Weber, Patty S.D.; Burton, Jeanne L.
Source:
Veterinary immunology and immunopathology 2002 v.86 no.3-4 pp. 159-175
ISSN:
0165-2427
Subject:
DNA-binding proteins; Northern blotting; animal health; blood serum; complementary DNA; cytochrome b; dairy cows; estradiol; gene expression; gene expression regulation; genes; immune response; mastitis; messenger RNA; metabolism; neutrophils; nucleic acid hybridization; nucleotide sequences; parturition; progesterone; radioimmunoassays; reverse transcriptase polymerase chain reaction; screening; sequence homology; steroid hormones; tricarboxylic acid cycle
Abstract:
... The periparturient dairy cow undergoes a plethora of physiological changes, including changes in the immune system that lead to profound effects on animal health. Of the immune cells affected at parturition, the neutrophil has been of particular interest due to its primary role in innate immune defense against mastitis. Immune functions of bovine neutrophils are known to be depressed around partur ...
DOI:
10.1016/S0165-2427(02)00020-X

8. Analysis of the repertoire of cattle CD4+ T cells reactive with bovine viral diarrhoea virus

Author:
Collen, T.; Carr, V.; Parsons, K.; Charleston, B.; Morrison, W.I.
Source:
Veterinary immunology and immunopathology 2002 v.87 no.3-4 pp. 235-238
ISSN:
0165-2427
Subject:
epitopes; recombinant antigens; alleles; synthetic peptides; Hepatitis C virus; cattle; cell-mediated immunity; Bovine viral diarrhea virus 1; CD4-positive T-lymphocytes; homozygosity; glycoproteins; CD8-positive T-lymphocytes
Abstract:
... Cell-mediated immunity and CD4+ cells in particular are important for the resolution of acute infection with non-cytopathic bovine viral diarrhoea virus (BVDV). CD4+ T cells were shown to recognise virus-infected and non-infectious-protein-pulsed APCs, whereas CD8+ T cells recognised only virus-infected APCs. T cell recognition was strain cross-reactive and MHC-restricted. Using native and recombi ...
DOI:
10.1016/S0165-2427(02)00088-0

9. Antibodies as effectors

Author:
Corbeil, L.B.
Source:
Veterinary immunology and immunopathology 2002 v.87 no.3-4 pp. 169-175
ISSN:
0165-2427
Subject:
antibodies; antigens; autoimmune diseases; cattle; pathogens
Abstract:
... Antibodies are critical in protection against extracellular microbial pathogens. Although antibodies also play a role in transplant/tumor rejection and in autoimmune disease, this paper focuses on defense against bovine infections. Effector mechanisms of different bovine isotypes, subisotypes and allotypes are discussed. The importance of antigen specificity is also stressed. ...
DOI:
10.1016/S0165-2427(02)00048-X

10. Antigen delivery systems and immunostimulation

Author:
Schijns, Virgil E.J.C.
Source:
Veterinary immunology and immunopathology 2002 v.87 no.3-4 pp. 195-198
ISSN:
0165-2427
Subject:
T-lymphocytes; antibodies; antigens; cytokines; immune response; lymph nodes; microorganisms; pathogens; vaccination; vaccine adjuvants; vaccine development; vaccines; virulence
Abstract:
... The immune system evolved to free the host from invading noxious pathogens. Vaccines are inoculated as a prophylactic measure in order to program the immune system for accelerated recognition and elimination of specific pathogens. During vaccination the immune system is exposed to attenuated or inactivated microorganisms, or their fragments. The immune response to these structures, in contrast to ...
DOI:
10.1016/S0165-2427(02)00072-7

11. B cell development and VDJ rearrangement in the fetal pig

Author:
Šinkora, Marek; Šinkorova, Jana; Butler, John E.
Source:
Veterinary immunology and immunopathology 2002 v.87 no.3-4 pp. 341-346
ISSN:
0165-2427
Subject:
B-lymphocytes; bone marrow; flow cytometry; liver; ontogeny; phenotype; pregnancy; swine; yolk sac
Abstract:
... Hematopoietic activity of the swine has been documented in three phases during fetal ontogeny. The hematopoietic system develops first in the yolk sac, then in fetal liver and finally in the bone marrow. Using flow cytometry (FCM) and molecular biological techniques we show that B-cell lymphogenesis and the appearance of B cells follows a pattern. First, VDJ rearrangement occurs at the 20th day of ...
DOI:
10.1016/S0165-2427(02)00062-4

12. B cell development in gut associated lymphoid tissues

Author:
Ratcliffe, Michael J.H.
Source:
Veterinary immunology and immunopathology 2002 v.87 no.3-4 pp. 337-340
ISSN:
0165-2427
Subject:
B-lymphocytes; bone marrow; bursa of Fabricius; chickens; embryogenesis; ileum; immunoglobulins; rodents; sheep; skeletal development
Abstract:
... B lymphocyte development can occur in a variety of anatomical sites. While typically considered to be a process that occurs in the bone marrow throughout life, it is becoming clear that gut associates sites of B cell development are critically important in many species of veterinary importance. Among these sites, the bursa of Fabricius in chickens and the ileal Peyer’s patches of sheep are among t ...
DOI:
10.1016/S0165-2427(02)00061-2

13. Bioinformatics and high throughput approach to create genomic resources for the study of bovine immunobiology

Author:
Coussens, Paul M.; Nobis, William
Source:
Veterinary immunology and immunopathology 2002 v.86 no.3-4 pp. 229-244
ISSN:
0165-2427
Subject:
infectious diseases; concanavalin A; complementary DNA; humans; microarray technology; cattle; bioinformatics; expressed sequence tags; reverse transcriptase polymerase chain reaction; gene expression; mice; pets; immune response; mononuclear leukocytes; RNA
Abstract:
... The advent of technologies such as real-time reverse transcriptase polymerase chain reaction (RT-PCR) and cDNA microarrays herald a new era in the study of biological systems. In immunobiology, these advances have begun to impact studies of infectious diseases, inflammatory processes, and immune cell function. However, a lack of genetic reagents for domestic and companion animals has precluded wid ...
DOI:
10.1016/S0165-2427(02)00005-3

14. Blood lymphocyte subsets in pigs vaccinated and challenged with Actinobacillus pleuropneumoniae

Author:
Appleyard, G.D.; Furesz, S.E.; Wilkie, B.N.
Source:
Veterinary immunology and immunopathology 2002 v.86 no.3-4 pp. 221-228
ISSN:
0165-2427
Subject:
Actinobacillus pleuropneumoniae; B-lymphocytes; CD4-positive T-lymphocytes; aerosols; antibodies; antigens; immunization; immunoglobulin M; phenotype; primary contact; secondary contact; swine
Abstract:
... Actinobacillus pleuropneumoniae bacterins do not induce protection in pigs while infection with low doses of the CM5 strain of A. pleuropneumoniae given by aerosol induces complete protection. To evaluate possible correlates of protection in blood lymphocyte subset phenotypes, pigs were treated with a commercial bacterin given intramuscularly, low dose (10(5) cfu/ml) aerosol infection with CM5 or ...
DOI:
10.1016/S0165-2427(02)00002-8

15. Bluetongue virus-induced activation of primary bovine lung microvascular endothelial cells

Author:
DeMaula, Christopher D.; Leutenegger, Christian M.; Jutila, Mark A.; MacLachlan, N. James
Source:
Veterinary immunology and immunopathology 2002 v.86 no.3-4 pp. 147-157
ISSN:
0165-2427
Subject:
Bluetongue virus; apoptosis; blood coagulation; bluetongue; cattle; endothelial cells; genes; inducible nitric oxide synthase; interleukin-1; interleukin-6; interleukin-8; messenger RNA; pathogenesis; prostaglandin synthase; quantitative polymerase chain reaction; sheep
Abstract:
... Bluetongue is an insect-transmitted viral disease of sheep and some species of wild ruminants. Infection of lung microvascular endothelial cells (ECs) is central to the pathogenesis of bluetongue virus (BTV) infection of ruminants, but it is uncertain as to why cattle are resistant to BTV-induced microvascular injury and bluetongue disease. Thus, in order to better understand the pathogenesis of B ...
DOI:
10.1016/S0165-2427(02)00012-0

16. CXCL8(3-73)K11R/G31P antagonizes the neutrophil chemoattractants present in pasteurellosis and mastitis lesions and abrogates neutrophil influx into intradermal endotoxin challenge sites in vivo

Author:
Li, Fang; Zhang, Xiaobei; Mizzi, Chris; Gordon, John R.
Source:
Veterinary immunology and immunopathology 2002 v.90 no.1-2 pp. 65-77
ISSN:
0165-2427
Subject:
CXCR1 receptor; CXCR2 receptor; antagonists; bacterial pneumonia; cattle; chemoattractants; chemotaxis; endotoxins; inflammation; interleukin-8; mastitis; neutrophils; pasteurellosis
Abstract:
... The ELR+ CXC chemokines are critical for protective neutrophil responses to most bacterial infections, but nevertheless can contribute importantly to the pathogenic effects of many inflammatory responses. We recently engineered a series of high affinity CXCL8/IL-8 antagonists, one of which, CXCL8(3–73)K11R/G31P, binds very strongly to neutrophils via the CXCR1 and CXCR2. Herein we show in competit ...
DOI:
10.1016/S0165-2427(02)00223-4

17. Candidate genes for resistance to Salmonella enteritidis colonization in chickens as detected in a novel genetic cross

Author:
Lamont, S.J.; Kaiser, M.G.; Liu, W.
Source:
Veterinary immunology and immunopathology 2002 v.87 no.3-4 pp. 423-428
ISSN:
0165-2427
Subject:
Salmonella Enteritidis; alleles; animal production; antibodies; backcrossing; chickens; chicks; disease resistance; esophagus; exposure pathways; food safety; gene interaction; heterozygosity; inbred lines; microsatellite repeats; mucosal immunity; salmonellosis; sires; spleen; vaccines; zoonoses; Iowa
Abstract:
... Salmonellosis is a zoonotic disease that is problematic for both animal production and food safety. A novel genetic cross, named the Iowa Salmonella response resource population (ISRRP), was established to elucidate the genetic control of resistance to Salmonella enteritidis (SE) colonization in young chicks, to characterize unique resistance alleles, and to estimate gene interaction effects. Outb ...
DOI:
10.1016/S0165-2427(02)00064-8

18. Cellular and molecular characterisation of the ovine rectal mucosal environment

Author:
Sedgmen, Bradley J.; Lofthouse, Shari A.; Scheerlinck, Jean-Pierre Y.; Meeusen, Els N.T.
Source:
Veterinary immunology and immunopathology 2002 v.86 no.3-4 pp. 215-220
ISSN:
0165-2427
Subject:
interleukin-2; interleukin-4; interleukin-8; messenger RNA; mucosa; phenotype; plasma cells; reverse transcriptase polymerase chain reaction; sheep; transforming growth factor beta; vaccines
Abstract:
... Immunohistological characterisation of ovine rectal tissue has revealed the presence of lymphoid follicles, predominantly in the submucosa, that closely resemble those found in intestinal Peyer’s patches (PPs). Distinct T (CD4+, CD8+, γδ-TCR+) and B (CD21+, CD45R+) lymphocyte staining patterns were observed within and around follicles of the rectal mucosa. In addition, IgA+ and IgE+ cells were als ...
DOI:
10.1016/S0165-2427(02)00011-9

19. Changes in macrophages in spleen and lymph nodes during acute African swine fever: expression of cytokines

Author:
Salguero, F.J.; Ruiz-Villamor, E.; Bautista, M.J.; Sánchez-Cordón, P.J.; Carrasco, L.; Gómez-Villamandos, J.C.
Source:
Veterinary immunology and immunopathology 2002 v.90 no.1-2 pp. 11-22
ISSN:
0165-2427
Subject:
African swine fever; apoptosis; blood serum; immunohistochemistry; interleukin-1beta; lymph nodes; lymphocytes; macrophages; pathogenesis; piglets; space and time; spleen; tumor necrosis factor-alpha; virulence; viruses
Abstract:
... To gain further insight into the pathogenesis of African swine fever (ASF), the cytokine expression by macrophages in spleen and lymph nodes were examined. Twenty-one piglets were inoculated with the highly virulent isolate Spain-70 of ASF virus and killed in groups at 1–7 post-inoculation days (pid). An increase in the immunohistochemical detection of proinflammatory monokines in spleen and renal ...
DOI:
10.1016/S0165-2427(02)00225-8

20. Changes in the leucocyte subpopulations of the palatine tonsillar crypt epithelium of pigs in response to Streptococcus suis type 2 infection

Author:
Salles, M.W.S.; Perez-Casal, J.; Willson, P.; Middleton, D.M.
Source:
Veterinary immunology and immunopathology 2002 v.87 no.1-2 pp. 51-63
ISSN:
0165-2427
Subject:
Streptococcus suis; T-lymphocytes; arthritis; epithelial cells; epithelium; innate immunity; macrophages; major histocompatibility complex; meningitis; monoclonal antibodies; neutrophils; pathogenesis; pathogens; septicemia; swine; tonsils; vaccination
Abstract:
... The tonsils are portal of entry and a site of multiplication and persistence for a variety of pathogens, including Streptococcus suis (S. suis), which is a common cause of meningitis, septicemia and arthritis in pigs. Understanding the early changes that occur in the first barrier of the tonsil, i.e. the crypt epithelium, in response to S. suis infection is critical in clarifying the pathogenesis ...
DOI:
10.1016/S0165-2427(02)00040-5