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You searched for: Author "Tang, Xiaoqian" Remove constraint Author: "Tang, Xiaoqian" Journal Veterinary immunology and immunopathology Remove constraint Journal: Veterinary immunology and immunopathology Subject Paralichthys olivaceus Remove constraint Subject: Paralichthys olivaceus Subject immune response Remove constraint Subject: immune response
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1. Immune response of flounder (Paralichthys olivaceus) was associated with the concentration of inactivated Edwardsiella tarda and immersion time

Author:
Tang, Xiaoqian,   et al.   ; Du, Yang; Sheng, Xiuzhen; Xing, Jing; Zhan, Wenbin;     Show all 5 Authors
Source:
Veterinary immunology and immunopathology 2015 v.167 no.1-2 pp. 44-50
ISSN:
0165-2427
Subject:
Edwardsiella tarda; Paralichthys olivaceus; antibodies; enzyme-linked immunosorbent assay; flounder; flow cytometry; immune response; kidneys; leukocytes; spleen; vaccination; vaccines
Abstract:
... In the optimization of immersion strategy, vaccine concentration and immersion time are two key factors needed to be considered, which largely determined the immune efficacy. In this work, the healthy flounder were vaccinated in formalin-killed Edwardsiella tarda at four concentrations (106, 107, 108, 109CFUml−1) for three immersion times (30, 60, 90min), respectively. At the 6th week post vaccina ...
DOI:
10.1016/j.vetimm.2015.07.002

2. Comparative study of the vaccine potential of six outer membrane proteins of Edwardsiella tarda and the immune responses of flounder (Paralichthys olivaceus) after vaccination

Author:
Tang, Xiaoqian,   et al.   ; Liu, Fuguo; Sheng, Xiuzhen; Xing, Jing; Zhan, Wenbin;     Show all 5 Authors
Source:
Veterinary immunology and immunopathology 2017 v.185 pp. 38-47
ISSN:
0165-2427
Subject:
Edwardsiella tarda; Paralichthys olivaceus; Toll-like receptor 2; Western blotting; antibodies; blood serum; flounder; gene expression regulation; genes; immune response; interferon-gamma; interleukin-1beta; lymphocytes; messenger RNA; outer membrane proteins; spleen; tumor necrosis factor-alpha; vaccination; vaccines
Abstract:
... To obtain effective vaccine candidates for Edwardsiella tarda, six outer membrane proteins (OMPs) of E. tarda were selected and recombinantly expressed. Western blotting analysis showed that flounder anti-E. tarda serum could recognize rOmpV, rOmpK, rOmpX and rOmpI, but not rOmpH and rOmpR. Immunization of flounder with different rOMPs resulted in high levels of protection following the E. tarda c ...
DOI:
10.1016/j.vetimm.2017.01.008

3. Dynamic distribution of formalin-inactivated Edwardsiella tarda in olive flounder (Paralichthys olivaceus) post intramuscular injection

Author:
Tang, Xiaoqian,   et al.   ; Zeng, Chuili; Du, Yang; Sheng, Xiuzhen; Xing, Jing; Zhan, Wenbin;     Show all 6 Authors
Source:
Veterinary immunology and immunopathology 2018 v.199 pp. 53-60
ISSN:
0165-2427
Subject:
Edwardsiella tarda; Paralichthys olivaceus; antigens; aquaculture; blood; flounder; fluorescent antibody technique; genes; immune response; intestines; intramuscular injection; kidneys; liver; muscles; physiological transport; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; skin (animal); spleen; tissue distribution; vaccination; vaccines
Abstract:
... Intramuscular (i.m.) injection is one of the common delivery methods of vaccination in aquaculture, which could induce an ideal immune protection to fish. In the present study, the olive flounders (Paralichthys olivaceus) were injected intramuscularly with 200 μl of three concentrations of formalin-inactivated Edwardsiella tarda bacterin (107, 108, 109 CFU ml−1) to investigate the transportation a ...
DOI:
10.1016/j.vetimm.2018.03.007