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- Author:
- Tang, Xiaoqian, et al. ; Liu, Fuguo; Sheng, Xiuzhen; Xing, Jing; Zhan, Wenbin; Show all 5 Authors
- Source:
- Veterinary immunology and immunopathology 2017 v.185 pp. 38-47
- ISSN:
- 0165-2427
- Subject:
- Edwardsiella tarda; Paralichthys olivaceus; Toll-like receptor 2; Western blotting; antibodies; blood serum; flounder; gene expression regulation; genes; immune response; interferon-gamma; interleukin-1beta; lymphocytes; messenger RNA; outer membrane proteins; spleen; tumor necrosis factor-alpha; vaccination; vaccines
- Abstract:
- ... To obtain effective vaccine candidates for Edwardsiella tarda, six outer membrane proteins (OMPs) of E. tarda were selected and recombinantly expressed. Western blotting analysis showed that flounder anti-E. tarda serum could recognize rOmpV, rOmpK, rOmpX and rOmpI, but not rOmpH and rOmpR. Immunization of flounder with different rOMPs resulted in high levels of protection following the E. tarda c ...
- DOI:
- 10.1016/j.vetimm.2017.01.008
-
https://dx.doi.org/10.1016/j.vetimm.2017.01.008
- Author:
- Tang, Xiaoqian, et al. ; Zeng, Chuili; Du, Yang; Sheng, Xiuzhen; Xing, Jing; Zhan, Wenbin; Show all 6 Authors
- Source:
- Veterinary immunology and immunopathology 2018 v.199 pp. 53-60
- ISSN:
- 0165-2427
- Subject:
- Edwardsiella tarda; Paralichthys olivaceus; antigens; aquaculture; blood; flounder; fluorescent antibody technique; genes; immune response; intestines; intramuscular injection; kidneys; liver; muscles; physiological transport; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; skin (animal); spleen; tissue distribution; vaccination; vaccines
- Abstract:
- ... Intramuscular (i.m.) injection is one of the common delivery methods of vaccination in aquaculture, which could induce an ideal immune protection to fish. In the present study, the olive flounders (Paralichthys olivaceus) were injected intramuscularly with 200 μl of three concentrations of formalin-inactivated Edwardsiella tarda bacterin (107, 108, 109 CFU ml−1) to investigate the transportation a ...
- DOI:
- 10.1016/j.vetimm.2018.03.007
-
https://dx.doi.org/10.1016/j.vetimm.2018.03.007
- Author:
- Tang, Xiaoqian, et al. ; Du, Yang; Sheng, Xiuzhen; Xing, Jing; Zhan, Wenbin; Show all 5 Authors
- Source:
- Veterinary immunology and immunopathology 2018 v.203 pp. 1-12
- ISSN:
- 0165-2427
- Subject:
- Scophthalmus maximus; Vibrio anguillarum; complementary DNA; foregut; gene expression regulation; genes; gills; hindgut; immunoglobulin T; intraperitoneal injection; kidneys; liver; midgut; molecular cloning; mucosal immunity; pathogens; plasma cells; skin (animal); spleen; stomach; turbot; vaccination
- Abstract:
- ... Immunoglobulins (Igs) are humoral mediators playing the prevailing role in the innate and adaptive immunities of jawed vertebrates and provide obligatory duties to protect the organism from a wide variety of pathogens. In the present study, the membrane-bound and secretory immunoglobulin T (mIgT and sIgT) genes of turbot (Scophthalmus maximus) were cloned for the first time with the intention of b ...
- DOI:
- 10.1016/j.vetimm.2018.07.011
-
https://dx.doi.org/10.1016/j.vetimm.2018.07.011