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1. Acetoacetate is a trigger of NLRP3 inflammasome activation in bovine peripheral blood mononuclear cells

Author:
Yuri Onizawa; Taiga Katoh; Ryotaro Miura; Kunitoshi Konda; Tatsuo Noguchi; Hisataka Iwata; Takehito Kuwayama; Seizo Hamano; Koumei Shirasuna
Source:
Veterinary immunology and immunopathology 2022 v.244 pp. 110370
ISSN:
0165-2427
Subject:
acetoacetic acid; blood plasma; caspase-1; estrous cycle; gene expression; immunopathology; inflammasomes; inflammation; ketone bodies; metabolomics; repeat breeder; reproductive disorders; secretion
Abstract:
... Repeat breeding, which is non-pregnancy following three or more breeding attempts, is a serious reproductive disorder in cattle. In the present study, metabolomic profiling was used to identify metabolites in the blood plasma of repeat breeder cows (RBCs) and non-RBCs. Metabolomic analysis showed that acetoacetate (AcAc), a ketone body, was detected in RBCs, but not in non-RBCs. In contrast, β-hyd ...
DOI:
10.1016/j.vetimm.2021.110370

2. Antibodies from healthy or paratuberculosis infected cows have different effects on Mycobacterium avium subspecies paratuberculosis invasion in a calf ileal loop model

Author:
Ana Jolly; Bárbara Fernández; Ana Stempler; Giselle Ingratta; Gabriela Postma; Juan Boviez; Daniel Lombardo; Silvia Hajos; Silvia L. Mundo
Source:
Veterinary immunology and immunopathology 2022 v.245 pp. 110381
ISSN:
0165-2427
Subject:
Mycobacterium avium subsp. paratuberculosis; calves; histology; histopathology; ileum; immunopathology; microbial load; models; paratuberculosis; protective effect; viability
Abstract:
... In this work, we used a calf ileal loop model to evaluate whether the preincubation of Mycobacterium avium subspecies paratuberculosis (MAP) with antibodies from healthy, MAP-positive or Lipoarabinomannan (LAM) immunized cows could affect the results of infection after 3.5 h. Bacterial load in tissue was assessed by Ziehl-Neelsen and by culture for each loop. MAP was detectable in all infected loo ...
DOI:
10.1016/j.vetimm.2022.110381

3. Assessment of heterologous lumpy skin disease vaccine-induced immunity in pregnant cattle vaccinated at different times of gestation period and their influence on maternally derived antibodies

Author:
Ahmed Gaber; Sherin Rouby; Amera Elsaied; Ahmed El-Sherif
Source:
Veterinary immunology and immunopathology 2022 v.244 pp. 110380
ISSN:
0165-2427
Subject:
cell-mediated immunity; gestation period; humoral immunity; immunopathology; longevity; lumpy skin disease; maternal immunity; parturition; pregnancy; sheep pox; vaccination; vaccines
Abstract:
... The present study aimed to evaluate the cell-mediated and the humoral immune response to Romanian sheep pox vaccine in pregnant cows (n = 12) vaccinated at different times of gestation period and the duration of maternal immunity in calves born to these cows. Evaluation of cellular immunity revealed an increase in lymphocytic proliferation that peaked at 10th day post vaccination (dpv) then gradua ...
DOI:
10.1016/j.vetimm.2021.110380

4. B cell memory responses induced by foot-and-mouth disease virus-like particles in BALB/c mice

Author:
Jia-huan Liu; Jun-juan Zhang; Wei-jian Han; Chuan Cui; Ming-zhu Li; Zhan-yun Tian; Ruo-man Bai; Li-min Li
Source:
Veterinary immunology and immunopathology 2022 v.250 pp. 110458
ISSN:
0165-2427
Subject:
antibodies; blood serum; bone marrow; foot-and-mouth disease; immunization; immunoglobulin G; immunopathology; interleukin-6; memory; spleen; vaccine development
Abstract:
... A challenging but critical question is that new foot-and-mouth disease (FMD) vaccines should be to induce B cell memory to provide antibodies for long-term protection. The maintenance of B cell memory is dependent on long-lived plasma cells (LLPCs) and memory B cells. We developed a chimeric FMDV virus-like particles (FMDV-VLPs), fusing VP1-VP4 into HBcAg. In our study, we investigated if or how l ...
DOI:
10.1016/j.vetimm.2022.110458

5. CD4 bovine gene: Differential polymorphisms among cattle breeds and a new tool for rapid identification

Author:
Cintia Hiromi Okino; Wilson Malagó Junior; Cintia Righetti Marcondes; Rodrigo Giglioti; Hélio José Montassier; Henrique Nunes de Oliveira; Márcia Cristina de Sena Oliveira
Source:
Veterinary immunology and immunopathology 2022 v.251 pp. 110462
ISSN:
0165-2427
Subject:
genes; genotyping; heterozygosity; homozygosity; hybrids; immunopathology; immunophenotyping; taurine; zebu
Abstract:
... Two mutations in the CD4 bovine gene (G>T/Q306H; A>C/K310N) were identified as causative for altered staining with anti-CD4 mAb #CC8. We developed a HRM qPCR for genotyping these mutations and compare with immunophenotyping in different cattle breeds. The assay distinguished five genotypes, B (homozygous, G/A) and C (heterozygous, G/A and T/C), found in taurine, A (homozygous, G/C) and D (heterozy ...
DOI:
10.1016/j.vetimm.2022.110462

6. Canine memory T-cell subsets in health and disease

Author:
Annie B. Bauman; Cambri E. Moeller; Aimee M. Soileau; Christine E. Goodermuth; Victoria R. Costa; Jessica C. Schaumburg; Cherie M. Pucheu-Haston; Nancy D. Welborn; Shannon D. Dehghanpir; Mark A. Mitchell; Sita S. Withers
Source:
Veterinary immunology and immunopathology 2022 v.246 pp. 110401
ISSN:
0165-2427
Subject:
T-lymphocytes; antigens; atopic dermatitis; dogs; drug therapy; flow cytometry; immunopathology; immunotherapy; inflammation; lymph nodes; lymphoma; memory; prednisone
Abstract:
... A more complete understanding of canine T-lymphocyte immunity is necessary for improving diagnostic and therapeutic approaches to canine diseases, developing cell-based canine immunotherapeutics, and evaluating dogs as large mammal models for comparative immunology research. The aim of this study was to utilize CD45RA (indicating antigen inexperience) and CD62L (indicating lymph node homing capabi ...
DOI:
10.1016/j.vetimm.2022.110401

7. Changes in rabbit lymphocyte subpopulations and activation following long-term administration of meloxicam

Author:
Pavla Hamouzová; Šárka Stehlíková; Kristína Řeháková; Vladimír Jekl; Jan Chloupek; Jaroslav Doubek
Source:
Veterinary immunology and immunopathology 2022 v.253 pp. 110496
ISSN:
0165-2427
Subject:
B-lymphocytes; analgesics; flow cytometry; hematology; immunology; immunopathology; meloxicam; rabbits
Abstract:
... Meloxicam is a commonly used analgesic in rabbits. However, its possible impact on lymphocyte subpopulations remained unknown. The aim of the study was to investigate a possible effect of long-term administration of meloxicam on rabbit lymphocyte subpopulations. The study included 8 rabbits given meloxicam orally once daily (1 mg/kg BW) for 14 days and 8 rabbits as a control group. Peripheral bloo ...
DOI:
10.1016/j.vetimm.2022.110496

8. Characterisation of dendritic cell frequency and phenotype in bovine afferent lymph reveals kinetic changes in costimulatory molecule expression

Author:
Sara Marzo; Mark Gray; Waywen Loh; Lindsey A. Waddell; C. Irene McGuinnes; Jayne C. Hope; Heather A. Mathie
Source:
Veterinary immunology and immunopathology 2022 v.243 pp. 110363
ISSN:
0165-2427
Subject:
T-lymphocytes; adaptive immunity; cattle; dendritic cells; flow cytometry; immunopathology; lymph; models; phenotype
Abstract:
... The bovine afferent lymphatic cannulation model allows collection of large volumes of afferent lymph and provides an opportunity to study lymphatic cells trafficking from the periphery directly ex-vivo. The technique requires surgical intervention, but influence of the procedure or time post-surgery on cells trafficking in the lymph has not been well documented. Here, we measured the volume of lym ...
DOI:
10.1016/j.vetimm.2021.110363

9. Chitosan-based formulation of hemagglutinin antigens for oculo-nasal booster vaccination of chickens against influenza viruses

Author:
Violetta Sączyńska; Agnieszka Romanik-Chruścielewska; Katarzyna Florys-Jankowska; Violetta Cecuda-Adamczewska; Małgorzata Kęsik-Brodacka
Source:
Veterinary immunology and immunopathology 2022 v.247 pp. 110406
ISSN:
0165-2427
Subject:
Escherichia coli; aluminum hydroxide; avian influenza; chitosan; glutamic acid; hemagglutination; hemagglutinins; immunopathology; pH; public health; recombinant proteins; secondary immunization; viral antigens
Abstract:
... Avian influenza viruses (AIVs) and especially highly pathogenic (HP) AIVs of the H5 and H7 subtypes are of both veterinary and public health concern worldwide. In response to the demand for effective vaccines against H5N1 HPAIVs, we produced recombinant protein based on hemagglutinin (HA), a protective viral antigen. A fragment of the HA ectodomain, with a multibasic cleavage site deletion, was ex ...
DOI:
10.1016/j.vetimm.2022.110406

10. Controlled release vaccine implants for delivery of booster immunisations

Author:
Freya A. Russell; Logan K. Trim; Flavia Medeiros Savi; Craig Simon; Tim R. Dargaville; Dietmar W. Hutmacher; Kenneth W. Beagley
Source:
Veterinary immunology and immunopathology 2022 v.253 pp. 110484
ISSN:
0165-2427
Subject:
T-lymphocytes; antibodies; antigens; blood serum; cell proliferation; immune response; immunopathology; livestock; polymers; secondary immunization; subcutaneous injection; tetanus; vaccination; vaccines; weight gain
Abstract:
... Most current animal vaccine regimes involve a primary vaccination followed sometime later by a booster vaccination. This presents challenges when vaccinating difficult to access animals such as livestock. Mustering livestock to deliver a vaccine boost is costly and stressful for animals. Thus, we have produced a platform system that can be administered at the same time as the priming immunisation ...
DOI:
10.1016/j.vetimm.2022.110484

11. Development of low-cost cage-like particles to formulate veterinary vaccines

Author:
Giuliana A. Lupi; Florencia X. Santiago Valtierra; Gabriel Cabrera; Roque Spinelli; Álvaro S. Siano; Verónica González; Antonio Osuna; Gerardo M. Oresti; Iván Marcipar
Source:
Veterinary immunology and immunopathology 2022 v.251 pp. 110460
ISSN:
0165-2427
Subject:
adjuvants; alum; cholesterol; delayed hypersensitivity; egg yolk; gas chromatography; immunoglobulin G; immunopathology; lysophospholipids; ovalbumin; saponins; thin layer chromatography
Abstract:
... Low-cost adjuvants are urgently needed for the development of veterinary vaccines able to trigger strong immune responses. In this work, we describe a method to obtain a low-cost cage-like particles (ISCOMATRIX-like) adjuvant useful to formulate veterinary vaccines candidates. The main components to form the particles are lipids and saponins, which were obtained from egg yolk by ethanolic extracti ...
DOI:
10.1016/j.vetimm.2022.110460

12. Differential detection of IgM and IgG antibodies to chimeric antigens in bovine tuberculosis

Author:
Archana A. Sridhara; Ashley Johnathan-Lee; Rubyat Elahi; Paul Lambotte; Javan Esfandiari; Maria Laura Boschiroli; Tanya J. Kerr; Michele A. Miller; Thomas Holder; Gareth Jones; H. Martin Vordermeier; Breanne N. Marpe; Tyler C. Thacker; Mitchell V. Palmer; W. Ray Waters; Konstantin P. Lyashchenko
Source:
Veterinary immunology and immunopathology 2022 pp. 110499
ISSN:
0165-2427
Subject:
Mycobacterium bovis; antibodies; blood serum; bovine tuberculosis; cattle; cross reaction; hosts; immunopathology; monitoring; rapid methods; risk; wildlife; France; Great Britain; South Africa
Abstract:
... Recent studies have suggested the potential of innovative serologic tests for accurate and rapid detection of bovine tuberculosis (bTB). Dual Path Platform (DPP) technology has been used to develop rapid animal-side antibody tests for Mycobacterium bovis infection in a range of livestock and wildlife host species. The present study evaluated diagnostic performance of DPP BovidTB IgM/IgG assay desi ...
DOI:
10.1016/j.vetimm.2022.110499

13. Dynamic changes in Holstein heifer circulatory stress biomarkers in response to lipopolysaccharide immune challenge

Author:
T. Sullivan; A. Sharma; K. Lamers; C. White; B.A. Mallard; A. Cánovas; N.A. Karrow
Source:
Veterinary immunology and immunopathology 2022 v.248 pp. 110426
ISSN:
0165-2427
Subject:
Holstein; acidosis; biomarkers; blood flow; blood serum; body temperature; climate change; cortisol; heat stress; immunopathology; inflammation; interleukin-10; intestinal mucosa; leukopenia; lipopolysaccharides; microRNA; septicemia; stress response; thrombocytopenia
Abstract:
... Dairy cattle routinely face a variety of stressors. For example, climate change has resulted in more frequent heat stress events that increase the incidence of bacterial infections by inducing conditions like leaky gut syndrome, whereby the integrity of the intestinal epithelium is compromised allowing for luminal bacteria and their membrane components to infiltrate the host’s bloodstream resultin ...
DOI:
10.1016/j.vetimm.2022.110426

14. Effect of aging on monocyte phagocytic and inflammatory functions, and on the ex vivo inflammatory responses to lipopolysaccharide, in horses

Author:
A.C. Herbst; S.E. Reedy; A.E. Page; D.W. Horohov; A.A. Adams
Source:
Veterinary immunology and immunopathology 2022 v.250 pp. 110459
ISSN:
0165-2427
Subject:
Escherichia coli; adiposity; body condition; ex vivo studies; flow cytometry; gene expression; genes; immunopathology; interleukin-18; interleukin-6; interleukin-8; lipopolysaccharides; monocytes; phagocytosis; young adults
Abstract:
... Studies investigating age-related changes in the function of monocytes are currently limited for horses. Thus, the main goal of this study was to determine the effect of aging on monocyte phagocytic capacity and pro-inflammatory cytokine responses to bacterial lipopolysaccharide (LPS). A second goal of this work was to examine the effect of aging on the inflammatory cytokine responses to LPS in a ...
DOI:
10.1016/j.vetimm.2022.110459

15. Effect of major versus minor mastectomy on host immunity in canine mammary cancer

Author:
Maria Karayannopoulou; Tilemachos Anagnostou; Apostolia Margariti; Maria Kritsepi-Konstantinou; Dimitra Psalla; Angelos-Lauris Thomas; Ioannis Savvas
Source:
Veterinary immunology and immunopathology 2022 v.246 pp. 110403
ISSN:
0165-2427
Subject:
T-lymphocytes; anesthesia; cell-mediated immunity; immunopathology; immunosuppression; innate immunity; mammary neoplasms (animal); metastasis; neutrophils; surgery
Abstract:
... Surgical procedures can affect host immunity proportionally to the extent of surgical trauma. In cancer cases, surgery-induced immunosuppression can potentially promote tumour metastasis. The aim of this study was to investigate, in bitches with malignant mammary tumours, whether major surgery (total unilateral mastectomy or bilateral regional mastectomy) has a more negative effect than minor surg ...
DOI:
10.1016/j.vetimm.2022.110403

16. Effects of immunostimulators of microbial origin on T cells of pigs vaccinated with attenuated vaccine against Aujeszkyʼs disease

Author:
Miroslav Andrišić; Irena Žarković; Ksenija Šandor; Anja Vujnović; Eleonora Perak Junaković; Krešo Bendelja; Ana Savić Mlakar; Nada Oršolić; Lidija Šver; Miroslav Benić; Svjetlana Terzić
Source:
Veterinary immunology and immunopathology 2022 v.243 pp. 110365
ISSN:
0165-2427
Subject:
Escherichia coli; Parapoxvirus; Propionibacterium granulosum; Suid alphaherpesvirus 1; T-lymphocytes; cell-mediated immunity; immunopathology; live vaccines; vaccination
Abstract:
... Aujeszky’s disease (AD) is a viral infectious disease caused by Suid herpesvirus 1 (SuHV-1). Vaccination and eradication of AD in domestic pigs is possible using marker vaccines with attenuated or inactivated SuHV-1, or subunit vaccines. However, vaccines with attenuated SuHV-1 have shown to be more potent in inducing strong cell-mediated immune response. The studies have shown that Parapoxvirus o ...
DOI:
10.1016/j.vetimm.2021.110365

17. Effects of intra-articular inoculation with Mycoplasma bovis on immunological responses in calf joints

Author:
Koji Nishi; Yuki Hirano; Ayano Sato; Ayako Eguchi; Kazuya Matsuda; Miyuki Toda; Takafumi Watanabe; Tomohito Iwasaki; Naoki Takahashi; Marina Hosotani; Reina Watanabe; Toshihide Kato; Hiromichi Ohtsuka; Satoshi Gondaira; Hidetoshi Higuchi
Source:
Veterinary immunology and immunopathology 2022 v.244 pp. 110364
ISSN:
0165-2427
Subject:
Mycoplasma bovis; arthritis; calves; cartilage; fibrin; immunohistochemistry; immunopathology; inflammation; interleukin-12; interleukin-17; interleukin-6; interleukin-8; lameness; messenger RNA; monitoring; phosphates; stifle; synovial fluid; transmission electron microscopy
Abstract:
... Mycoplasma arthritis that caused by Mycoplasma bovis exhibit severe lameness. This disease is difficult to cure with antibiotics, but the detailed pathological mechanisms have not been fully clarified. In this study, we examined the effects of intra-articular inoculation with M. bovis on immunological responses in calf joints. We inoculated three calves each with M. bovis or phosphate buffer salin ...
DOI:
10.1016/j.vetimm.2021.110364

18. Evaluation of peripheral blood polymorphonuclear cell functions after an oral carbohydrate overload in obese and insulin dysregulated horses

Author:
Constanza Salinas; Gabriel Espinosa; Natalia Morales; Claudio Henríquez; Gabriel Morán; Gonzalo Gajardo; Rafael A. Burgos; Benjamin Uberti
Source:
Veterinary immunology and immunopathology 2022 v.250 pp. 110455
ISSN:
0165-2427
Subject:
adults; apoptosis; blood; blood glucose; body condition; glucose; hyperglycemia; immunopathology; insulin; insulin resistance; obesity; phagocytosis; reactive oxygen species; statistical models
Abstract:
... Obesity and insulin dysregulation (ID) are increasingly prevalent conditions in equid populations worldwide. Immune impairment is well described in humans with metabolic dysfunction and is reported but still incompletely understood in horses. This study evaluated the effect of acute induced transient hyperglycemia on apoptosis, phagocytosis and oxidative burst activity of peripheral blood polymorp ...
DOI:
10.1016/j.vetimm.2022.110455

19. Evaluation of the efficacy of cow colostrum in the treatment and its effect on serum proteomes in calves with cryptosporidiosis

Author:
Yiğit KAÇAR; Ahmet Tarık BAYKAL; Levent AYDIN; Hasan BATMAZ
Source:
Veterinary immunology and immunopathology 2022 v.248 pp. 110429
ISSN:
0165-2427
Subject:
Cryptosporidium; Holstein; amyloid; blood serum; calves; cow colostrum; cryptosporidiosis; gamma-glutamyltransferase; immunoglobulin G; immunopathology; oocysts; protein content; proteome; proteomics; sodium bicarbonate; zinc
Abstract:
... This study aimed to investigate the effect of using high-quality colostrum in addition to paromomycin on the treatment outcomes and serum proteomes of calves naturally affected by cryptosporidiosis. Thirty Holstein calves infected with only Cryptosporidium spp. were divided into three equal groups. Calves in the PC group received paromomycin orally at a dose of 100 mg/kg once daily for 5 days. Cal ...
DOI:
10.1016/j.vetimm.2022.110429

20. Evidence for transfer of maternal antigen specific cellular immunity against Mycobacterium avium ssp. paratuberculosis via colostrum in a goat twin model

Author:
L. Robbers; R. van de Mheen; L. Benedictus; R. Jorritsma; M. Nielen; H.J.C. Bijkerk; S.G. van der Grein; L. Ravesloot; A.P. Koets
Source:
Veterinary immunology and immunopathology 2022 v.246 pp. 110402
ISSN:
0165-2427
Subject:
Mycobacterium avium subsp. paratuberculosis; blood serum; cell-mediated immunity; cow colostrum; epithelium; histology; immune response; immunopathology; injection site; intestines; models; tuberculin; vaccines
Abstract:
... Colostrum intake is one of the most important factors in neonatal health in ruminants, mainly because of its unique immunological properties. Both in practice as well as in research, the attention of lactogenic immunity is focused on the importance of colostral antibodies and less attention is given to the functional role of maternal cells in colostrum. Here we study the transfer of maternal leuko ...
DOI:
10.1016/j.vetimm.2022.110402

21. H3N2 canine influenza virus NS1 protein inhibits canine NLRP3 inflammasome activation

Author:
Pan Tao; Zhangyong Ning; Pei Zhou; Weiqi Xiao; Guiping Wang; Shoujun Li; Guihong Zhang
Source:
Veterinary immunology and immunopathology 2022 v.252 pp. 110483
ISSN:
0165-2427
Subject:
Influenza A virus; canine influenza; caspase-1; dogs; immunopathology; inflammasomes; inflammation; innate immunity; interleukin-18; oligomerization; transcription (genetics)
Abstract:
... Inflammation is an innate immune response of the body against pathogens and other irritants. The NLRP3 (NACHT, LRR and PYD domains-containing protein 3) inflammasome is a major player in the inflammatory response against pathogenic microorganisms. In this study, we analyzed the relationship between the NLRP3 inflammasome and the influenza virus NS1 protein, which is involved in host immune escape. ...
DOI:
10.1016/j.vetimm.2022.110483

22. Immunoproteomic analysis of the secretome of bovine-adapted strains of Staphylococcus aureus demonstrates a strain-specific humoral response

Author:
Shauna D. Drumm; Paul Cormican; Padraig O’Boyle; Rebecca A. Owens; Jennifer Mitchell; Orla M. Keane
Source:
Veterinary immunology and immunopathology 2022 v.249 pp. 110428
ISSN:
0165-2427
Subject:
Staphylococcus aureus; antigens; blood serum; cows; genes; humoral immunity; immunopathology; mass spectrometry; molecular weight; pathogens; vaccines; virulence
Abstract:
... Staphylococcus aureus is a common pathogen associated with bovine intramammary infection. A number of distinct S. aureus lineages are associated with such infections although there is a dearth of knowledge regarding the major immunogenic antigens associated with each lineage and whether these antigens provide protection against heterologous strains. Identification of the major immunogenic antigens ...
DOI:
10.1016/j.vetimm.2022.110428

23. Infection by bovine alphaherpesvirus types 1 and 5 induces IFN-λ3 expression in neuronal-type cells and bovine neural tissues

Author:
J.J. Rosales; M.V. Nieto Farías; M. Burucúa; M.S. Marin; S.E. Pérez
Source:
Veterinary immunology and immunopathology 2022 v.245 pp. 110391
ISSN:
0165-2427
Subject:
Bovine alphaherpesvirus 1; bioactive properties; brain; epithelium; ganglia; immunopathology; innate immunity; transcription (genetics); virus replication; viruses
Abstract:
... Type III interferons (IFNs) are components of the innate immunity, with IFN lambda- (λ)3 having the most potent bioactivity in humans. IFN-λ has a predominant role in epithelial cells. However, antiviral function in certain infections of the central nervous system has also been demonstrated. IFN-λ3 expression in neural tissues of cattle has not been investigated. Thus, the aim of this study was to ...
DOI:
10.1016/j.vetimm.2022.110391

24. Influenza A pathway analysis of highly pathogenic avian influenza virus (H5N1) infection in genetically disparate Ri chicken lines

Author:
Jiae Lee; Yeojin Hong; Thi Hao Vu; Sooyeon Lee; Jubi Heo; Anh Duc Truong; Hyun S. Lillehoj; Yeong Ho Hong
Source:
Veterinary immunology and immunopathology 2022 v.246 pp. 110404
ISSN:
0165-2427
Subject:
Influenza A virus; RNA; chickens; cytokines; gene expression; gene expression regulation; immune system; immunity; immunopathology; influenza; interferons; quantitative polymerase chain reaction; reverse transcription; viruses; Vietnam
Abstract:
... Influenza A/H5N1 virus is a highly pathogenic (HPAIV) and contagious zoonotic virus that can be transmitted to humans. In the present study, infection with this virus and differential gene expression analyses were carried out with genetically resistant and susceptible Ri chicken lines that are native to Vietnam. A total of 20 four-week-old Ri chickens from each line were inoculated with the highly ...
DOI:
10.1016/j.vetimm.2022.110404

25. Insufficiency of CD205-positive cortical thymic epithelial cells in immature Japanese Black cattle with severe thymic abnormalities and poor prognosis

Author:
Shoichi Wakitani; Risako Kawabata; Masahiro Yasuda
Source:
Veterinary immunology and immunopathology 2022 v.245 pp. 110379
ISSN:
0165-2427
Subject:
Wagyu; cortex; epithelium; histopathology; immunohistochemistry; immunopathology; interleukin-7; prognosis; surveys
Abstract:
... To investigate the involvement of thymic function in the development of diseases with poor prognosis in calves, this study conducted a survey for the assessment of thymus cell composition in immature Japanese Black cattle with poor prognosis. Histopathological evaluation of 47 cattle showed signs of acute thymic involution in most cases. Less than half of the cases had a cortex predominant over th ...
DOI:
10.1016/j.vetimm.2021.110379

26. Interleukin-1Ra (Il-1Ra) and serum cortisol level relationship in horse as dynamic adaptive response during physical exercise

Author:
Francesca Arfuso; Elisabetta Giudice; Michele Panzera; Maria Rizzo; Francesco Fazio; Giuseppe Piccione; Claudia Giannetto
Source:
Veterinary immunology and immunopathology 2022 v.243 pp. 110368
ISSN:
0165-2427
Subject:
Thoroughbred; antagonists; athletic performance; blood serum; cortisol; erythrocytes; exercise; hematocrit; hemoglobin; horses; immunopathology; interleukin-1; physical health; stress response
Abstract:
... The monitoring of endocrine and immunologic markers during exercise is of paramount importance to assess and/or maintain the physical well-being of athletes as well as to optimize the athletic performance. This study aimed to investigate the linkage between acute stress response and immune status in Thoroughbred horses competing in an official 1300-m race. From 10 horses blood was collected 1 week ...
DOI:
10.1016/j.vetimm.2021.110368

27. Measurement of canine Th17 cells by flow cytometry

Author:
A. Knebel; A. Kämpe; R. Carlson; K. Rohn; A. Tipold
Source:
Veterinary immunology and immunopathology 2022 v.243 pp. 110366
ISSN:
0165-2427
Subject:
CD4-positive T-lymphocytes; autoimmune diseases; dogs; flow cytometry; immunology; immunopathology; inflammation
Abstract:
... Th17 cells are T helper cells which play an important role during inflammation and autoimmune disease. To investigate the role of these cells in diseases in dogs in a clinical setting, methods for fast identification had to be established. Th17 cells are a rare cell population, for their measurement stimulation is recommended. To examine more samples simultaneously and to receive a relatively high ...
DOI:
10.1016/j.vetimm.2021.110366

28. Oxidative stress in neonatal lambs and its relation to health status and passive colostral immunity

Author:
Erhan Gökçe; Pınar Cihan; Onur Atakişi; Ali Haydar Kirmizigül; Hidayet Metin Erdoğan
Source:
Veterinary immunology and immunopathology 2022 v.251 pp. 110470
ISSN:
0165-2427
Subject:
antioxidant activity; blood; colostral immunity; colostrum; crossbreds; health status; immunopathology; ingestion; oxidants; oxidative stress; postpartum period
Abstract:
... Oxidative stress (OS) related to birth in ruminants may increase susceptibility to diseases. However, information about the physiological changes related to OS in lambs is limited. This study was designed to evaluate changes in OS parameters [total antioxidant status (TAS, mmol Trolox Equiv./L), total oxidant status (TOS, μmol H₂O₂ Equiv./L)] and calculated oxidative stress index (OSi = TOS/TAS *  ...
DOI:
10.1016/j.vetimm.2022.110470

29. Pharmacological inhibition of the mTOR pathway alters phenotype and cytokine expression in bovine monocyte-derived dendritic cells

Author:
Anja Sipka; Thomas Weichhart; Sabine Mann
Source:
Veterinary immunology and immunopathology 2022 v.249 pp. 110441
ISSN:
0165-2427
Subject:
Escherichia coli; antigen presentation; cell culture; early lactation; flow cytometry; gene expression; immune response; immunopathology; infectious diseases; inflammation; interleukin-10; lipopolysaccharides; monocytes; nutrient availability; pathogens; phenotype; rapamycin
Abstract:
... Epidemiological studies have long demonstrated the association of nutrient status and immune dysfunction in dairy cows. Postpartum dairy cows experiencing a nutrient deficit show a propensity for increased inflammatory response, decreased pathogen clearance, and increased incidence of infectious disease. Studies in cows and other species show that the nutrient sensing mechanistic target of rapamyc ...
DOI:
10.1016/j.vetimm.2022.110441

30. Potential for improved detection of bovine tuberculosis by targeting combined blood biomarkers in multi-test algorithms

Author:
Archana A. Sridhara; Ashley Johnathan-Lee; Rubyat Elahi; Alina Sikar-Gang; Paul Lambotte; Javan Esfandiari; Lucia de Juan; Christian Gortazar; Breanne N. Marpe; Tyler C. Thacker; Mitchell V. Palmer; W. Ray Waters; Konstantin P. Lyashchenko
Source:
Veterinary immunology and immunopathology 2022 v.248 pp. 110419
ISSN:
0165-2427
Subject:
Mycobacterium bovis; antibodies; biomarkers; blood; bovine tuberculosis; cattle; diagnostic sensitivity; immunopathology; interferon-gamma; tuberculin; Spain
Abstract:
... Bovine tuberculosis (bTB) control programs can be improved by combined use of tests for humoral and cell-mediated immune responses targeting multiple biomarkers of Mycobacterium bovis. To further the diagnostic benefits of this approach, we used Dual Path Platform (DPP) technology to test sera from cattle with naturally acquired bTB in the United States (US) and Spain for the presence of M. bovis ...
DOI:
10.1016/j.vetimm.2022.110419

31. Protective effects of chicoric acid on polyinosinic-polycytidylic acid exposed chicken hepatic cell culture mimicking viral damage and inflammation

Author:
Patrik Tráj; Eva Madeleine Herrmann; Csilla Sebők; Júlia Vörösházi; Máté Mackei; Péter Gálfi; Ágnes Kemény; Zsuzsanna Neogrády; Gábor Mátis
Source:
Veterinary immunology and immunopathology 2022 v.250 pp. 110427
ISSN:
0165-2427
Subject:
Asteraceae; acetylcysteine; biochemical pathways; chickens; coculture; culture media; dose response; double-stranded RNA; free radicals; immunopathology; inflammation; interleukin-6; interleukin-8; lactate dehydrogenase; polyinosinic-polycytidylic acid; poultry industry; viruses
Abstract:
... Virus induced damage triggered by excessive inflammation and free radical production is a major threat in the poultry industry, leading to low productivity even in vaccinated flocks. The purpose of the study was to induce inflammation with the viral double-stranded RNA analog polyinosinic-polycytidylic acid (poly I:C) on chicken primary hepatocyte – non-parenchymal cell co-cultures to investigate ...
DOI:
10.1016/j.vetimm.2022.110427

32. Reduced capability of refrigerated white rhinoceros whole blood to produce interferon-gamma upon mitogen stimulation

Author:
Rebecca Dwyer; Carmel Witte; Peter Buss; Tebogo Manamela; Leana Freese; Guy Hausler; Wynand J. Goosen; Michele Miller
Source:
Veterinary immunology and immunopathology 2022 v.252 pp. 110485
ISSN:
0165-2427
Subject:
Ceratotherium simum; Mycobacterium bovis; absorbance; blood; immunopathology; interferon-gamma; males; mitogens; monitoring; national parks; refrigeration
Abstract:
... Ante-mortem surveillance for Mycobacterium bovis (M. bovis) infection in the Kruger National Park (KNP) rhinoceros population currently relies on results from the QuantiFERON-TB Gold (In-Tube) Plus (QFT)–interferon gamma (IFN-γ) release assay (IGRA). However, same-day processing of rhinoceros blood samples for this test is a logistical challenge. Therefore, a pilot study was performed to compare m ...
DOI:
10.1016/j.vetimm.2022.110485

33. Residue of thiram in food, suppresses immune system stress signals and disturbs sphingolipid metabolism in chickens

Author:
Yingwei Liu; Jiangnan Yi; Yuanliang Li; Riaz Hussain; Shanshan Zhu; Yangwei Li; Zhuanxu Ouyang; Khalid Mehmood; Lianmei Hu; Jiaqing Pan; Zhaoxin Tang; Ying Li; Hui Zhang
Source:
Veterinary immunology and immunopathology 2022 v.247 pp. 110415
ISSN:
0165-2427
Subject:
angiogenesis; apoptosis; autophagy; cell membranes; immunology; immunopathology; immunotoxicity; metabolites; metabolome; necropsy; pollution; public health; sphingolipids; spleen; sulfur; thiram
Abstract:
... Thiram, a well-known sulfur containing organic compound is frequently and extensively used in agriculture because of high biological activity to control different pests. In certain cases, due to long persistence in the environment pesticides and other environmental contaminants induce undesirable toxic impacts to public health and environment. To ascertain the potential mechanisms of toxicity of t ...
DOI:
10.1016/j.vetimm.2022.110415

34. Sheep vaccinated against paratuberculosis have increased levels of B cells infiltrating the intestinal tissue

Author:
Hannah B. Pooley; Richard J. Whittington; Douglas J. Begg; Auriol C. Purdie; Karren M. Plain; Kumudika de Silva
Source:
Veterinary immunology and immunopathology 2022 v.252 pp. 110482
ISSN:
0165-2427
Subject:
Mycobacterium avium subsp. paratuberculosis; enteritis; ileum; immune response; immunohistochemistry; immunopathology; macrophages; paratuberculosis; sheep; vaccination; vaccine development; vaccines
Abstract:
... Systemic immunisation delivered subcutaneously is currently used to control paratuberculosis, a chronic enteritis of ruminants caused by Mycobacterium avium subspecies paratuberculosis (MAP). These vaccines do not provide complete protection and a small cohort of animals still succumb to clinical disease. The aim of this study was to assess mycobacterial infection site-specific variations in immun ...
DOI:
10.1016/j.vetimm.2022.110482

35. Systemic murine cathelicidin CRAMP safely attenuated colonic neutrophil infiltration in pigs

Author:
Cristina C. Fodor; Robert McCorkell; Greg Muench; Eduardo R. Cobo
Source:
Veterinary immunology and immunopathology 2022 v.249 pp. 110443
ISSN:
0165-2427
Subject:
cathelicidins; colitis; colon; enteropathogens; epithelium; glycosylation; homeostasis; immunology; immunopathology; intraperitoneal injection; mice; mucins; mucus; multiple drug resistance; neutrophils
Abstract:
... Post-weaning diarrheic colitis, often caused by enteropathogens, are severe and potentially lethal diseases in young pigs. Conventional treatment with antibiotics is problematic due to increasing prevalence of multi-drug resistant bacteria. Few alternative treatments exist, so development of antibiotic-free therapies is urgently needed for livestock. Cathelicidin peptides, produced by epithelial c ...
DOI:
10.1016/j.vetimm.2022.110443

36. Thymic changes due to leishmaniasis in dogs: An immunohistochemical study

Author:
Giulia Gonçalves Jussiani; Karen Santos Março; Paulo Henrique Leal Bertolo; Rosemeri de Oliveira Vasconcelos; Gisele Fabrino Machado
Source:
Veterinary immunology and immunopathology 2022 v.247 pp. 110416
ISSN:
0165-2427
Subject:
Leishmania infantum; apoptosis; atrophy; collagen; death; epithelium; histopathology; immune response; immunohistochemistry; immunopathology; leishmaniasis; macrophages; mitosis; morphometry; parasites; pathophysiology; thymocytes; tissue distribution
Abstract:
... The thymus is necessary for the differentiation of T cells, a process that is regulated by the type of antigens found in thymocytes, the environment of surrounding cells and the thymus architecture. There is evidence that infectious diseases may result in morphological changes in this organ, such as premature atrophy and decreased thymocyte proliferation, that can affect the immune response. We ch ...
DOI:
10.1016/j.vetimm.2022.110416

37. Tracheal cellular immune response in chickens inoculated with Mycoplasma synoviae vaccine, MS-H or its parent strain 86079/7NS

Author:
Oluwadamilola S. Omotainse; Nadeeka K. Wawegama; Sathya N. Kulappu Arachchige; Mauricio J. C. Coppo; Paola K. Vaz; Andrew P. Woodward; Somayeh Kordafshari; Mirjana Bogeski; Mark Stevenson; Amir H. Noormohammadi; Andrew W. Stent
Source:
Veterinary immunology and immunopathology 2022 v.251 pp. 110472
ISSN:
0165-2427
Subject:
Mycoplasma synoviae; cell-mediated immunity; immunopathology; interleukin-17; light microscopy; live vaccines; macrophages; mucosa; respiratory tract diseases; specific pathogen-free animals; vaccination
Abstract:
... Mycoplasma synoviae causes respiratory tract disease in chickens characterised by mild to moderate lymphoplasmacytic infiltration of the tracheal mucosa. MS-H (Vaxsafe1 MS, Bioproperties Pty Ltd.) is an effective live attenuated vaccine for M. synoviae, but the immunological basis for its mechanism of protection has not been investigated, and the phenotypes of lymphocytes and associated cytokines ...
DOI:
10.1016/j.vetimm.2022.110472

38. An allergen-fused dendritic cell-binding peptide enhances in vitro proliferation of equine T-cells and cytokine production

Author:
Anja Ziegler; Judith Olzhausen; Eman Hamza; Ana Stojiljkovic; Michael H. Stoffel; Mattia Garbani; Claudio Rhyner; Eliane Marti
Source:
Veterinary immunology and immunopathology 2022 v.243 pp. 110351
ISSN:
0165-2427
Subject:
Culicoides; T-lymphocytes; Toll-like receptor 4; adjuvants; agonists; allergens; asthma; cancer therapy; cell proliferation; dendritic cells; dermatitis; horses; humans; immune response; immunogenicity; immunomodulators; immunopathology; immunotherapy; insects; interleukin-10; interleukin-17; interleukin-4; mice; oligodeoxyribonucleotides; ovalbumin
Abstract:
... Allergen-specific immunotherapy (AIT) constitutes the only curative approach for allergy treatment. There is need for improvement of AIT in veterinary medicine, such as in horses suffering from insect bite hypersensitivity, an IgE-mediated dermatitis to Culicoides. Dendritic cell (DC)-targeting represents an efficient method to increase antigen immunogenicity. It is studied primarily for its use i ...
DOI:
10.1016/j.vetimm.2021.110351

39. A clonality assay in canine B cell tumors targeting the immunoglobulin light chain lambda locus

Author:
Masamine Takanosu; Yumiko Kagawa
Source:
Veterinary immunology and immunopathology 2022 v.253 pp. 110498
ISSN:
0165-2427
Subject:
B-lymphocytes; DNA; antigens; capillary electrophoresis; databases; dogs; genes; immunogenetics; immunoglobulin heavy chains; immunoglobulin light chains; immunopathology; inflammation; loci; lymph; lymphoma; polymerase chain reaction
Abstract:
... Clonality assays for antigen receptor rearrangement have been used as adjunct examinations of lymphoproliferative diseases. These assays have been useful for differentiation between inflammation and clonal expansion of lymphocytes. Whereas the immunoglobulin heavy chain (IGH) and immunoglobulin light chain kappa (IGK) loci have been targeted in canine clonality assays previously, the immunoglobuli ...
DOI:
10.1016/j.vetimm.2022.110498

40. A ferret model of immunosuppression induced with dexamethasone

Author:
Anna Hundakova; Lenka Leva; Miroslav Toman; Zdenek Knotek
Source:
Veterinary immunology and immunopathology 2022 v.243 pp. 110362
ISSN:
0165-2427
Subject:
Phytolaccaceae; chemiluminescence; concanavalin A; dexamethasone; ferrets; humans; immunopathology; immunosuppression; ingestion; mitogens; monocytes; neutrophils; phagocytosis; phytohemagglutinin; reactive oxygen species
Abstract:
... Ferrets are nowadays frequently used as animal models for biomedical purposes; in many cases, immunosuppression of experimental animals is necessary. The aim of this study was to evaluate the effect of intramuscular dexamethasone administration (2 mg/kg as the initiation dose continued with 1 mg/kg q 12 h applied 5 times) on ferret’s immune system. In comparison with ferrets which received the sal ...
DOI:
10.1016/j.vetimm.2021.110362

41. A 14 bp indel polymorphism in the promoter region is associated with different responses to porcine circovirus type 2 infection by regulating MRC1 transcription

Author:
Hao Liu; Changying Wang; Gen Liu; Yanping Li; Li Kang; Yi Sun; Yunliang Jiang
Source:
Veterinary immunology and immunopathology 2021 v.234 pp. 110202
ISSN:
0165-2427
Subject:
Duroc; Porcine circovirus-2; genes; heart; immune response; immunopathology; kidneys; landraces; large intestine; liver; luciferase; lungs; lymph nodes; mannose; pathogens; promoter regions; small intestine; spleen; swine; transcription (genetics)
Abstract:
... Mannose receptor, C type 1 (MRC1) is a key factor in regulating the body's immune response to resist pathogen invasions. In this study, mRNA expressions of MRC1 gene in nine porcine organs/tissues were compared between Laiwu (LW) and Yorkshire × Landrace crossbred (YL) pigs prior to and post PCV2 infection. We found that, for pigs uninfected with PCV2, MRC1 mRNA expressions in the lung, spleen, la ...
DOI:
10.1016/j.vetimm.2021.110202

42. APB-F1, a long-acting feline granulocyte colony-stimulating factor fusion protein, created by exploiting FL335, a chimeric Fab specific for feline serum albumin

Author:
Hyun-jin Chi; Mihyun Park; Jae-kyu Han; Sun-mi Kim; SeungGoo Kang; Jin-hyuk Yang; Sang-hoon Cha
Source:
Veterinary immunology and immunopathology 2021 v.240 pp. 110322
ISSN:
0165-2427
Subject:
Cricetulus griseus; antibodies; bioactive properties; blood serum; cats; granulocyte colony-stimulating factor; half life; humans; immunopathology; neutropenia; neutrophils; pharmacodynamics; pharmacokinetics; recombinant fusion proteins; serum albumin; therapeutics
Abstract:
... Off-label use of a human granulocyte colony stimulating factor (hG-CSF) has been allowed to treat dogs and cats with neutropenia. However, repeated administration of hG-CSF induces undesirable anti-drug antibody (ADA) responses, implying the necessity of animal-derived G-CSF as a therapeutic reagent, preferably with a long-acting capability. Herein, we generated a recombinant fusion protein by gen ...
DOI:
10.1016/j.vetimm.2021.110322

43. Activation of canine neutrophils by platelet-activating factor

Author:
Ken Okabayashi; Shuichiro Kanai; Fumihiko Katakura; Riku Takeuchi; Takashi Yamauchi; Shunya Nakayama; Rie Kinoshita; Hiroshi Koie; Takanori Narita
Source:
Veterinary immunology and immunopathology 2021 v.241 pp. 110336
ISSN:
0165-2427
Subject:
calcium; dogs; endoplasmic reticulum; immunopathology; inflammation; inositols; neutrophils; phagocytosis; platelet aggregation; platelet-activating factor; respiratory burst; signal transduction; suramin
Abstract:
... Neutrophils are essential for innate immunity as the first line of defence. Neutrophils act as phagocytic white blood cells to kill bacteria and other microorganisms. A strong respiratory burst of neutrophils, dependent on reactive oxygen species, is produced during phagocytosis. Platelet-activating factor (PAF) is a signalling molecule with several prominent roles in tissue injury, inflammation, ...
DOI:
10.1016/j.vetimm.2021.110336

44. Activation of chicken gamma-delta T lymphocytes by a purified ulvan extract

Author:
Nathalie Guriec; Frédérick Bussy; Christelle Gouin; Olivier Mathiaud; Matthieu Le Goff; Jacques Delarue; Pi Nyvall Collén
Source:
Veterinary immunology and immunopathology 2021 v.237 pp. 110255
ISSN:
0165-2427
Subject:
Avian orthoavulavirus 1; Salmonella; Ulva; chickens; cytotoxicity; flow cytometry; heterophils; immune response; immunopathology; intestines; major histocompatibility complex; monocytes; polysaccharides; spleen; vaccination
Abstract:
... Chicken γδ T lymphocytes are present in a variety of tissues such as blood, spleen and intestine. They constitute a major cytotoxic population. In chicken, Salmonella immunization as well as vaccination against Newcastle disease virus are accompanied by an increase of γδ T lymphocytes in peripheral blood, which may be activated, and thus represent a protective immune response. It has been publishe ...
DOI:
10.1016/j.vetimm.2021.110255

45. Anti-inflammatory activity of citrus pectin on chicken monocytes' immune response

Author:
G. Ávila; D. De Leonardis; G. Grilli; C. Lecchi; F. Ceciliani
Source:
Veterinary immunology and immunopathology 2021 v.237 pp. 110269
ISSN:
0165-2427
Subject:
Citrus; Escherichia coli; anti-inflammatory activity; apoptosis; biochemical pathways; caspase-3; chemotaxis; dietary fiber; digestibility; dose response; energy; galacturonic acid; immune response; immunopathology; monocytes; pectins; phagocytosis; toxicity testing; viability
Abstract:
... Pectin is a dietary fibre composed of galacturonic acid, primarily found in the citrus fruits' cell walls. Citrus pectin (CP) has demonstrated antioxidative, anticancer, and anti-inflammatory properties in humans and animals. In broilers, CP supplementation improves energy utilization and nutrient digestibility, but limited information on its effects on chicken immunity is available so far. This s ...
DOI:
10.1016/j.vetimm.2021.110269

46. Antibodies that react with bovine T lymphocytes expressing the T cell receptor β chain subgroup BV20 inhibit antigen recognition

Author:
W. Ivan Morrison; Tara Sheldrake; Timothy Connelley
Source:
Veterinary immunology and immunopathology 2022 v.246 pp. 110392
ISSN:
0165-2427
Subject:
antigens; cattle; cytotoxicity; gene duplication; genes; immunopathology
Abstract:
... In recent years, molecular studies have provided detailed information on the bovine T cell receptor (TCR) variable gene repertoire, both in resting T cells and during T cell responses. However, studies of the biological function of the receptor have been hampered by a lack of reagents that recognise the protein. Herein, we describe the characterisation of two antibodies (IL-A47 and IL-A98) that re ...
DOI:
10.1016/j.vetimm.2022.110392

47. Assessment of three commercial ELISAs for the detection of antibodies against Porcine epidemic diarrhea virus at different stages of the immune response

Author:
I. Díaz; J. Pujols; E. Cano; R. Cuadrado; N. Navarro; E. Mateu; M. Martín
Source:
Veterinary immunology and immunopathology 2021 v.234 pp. 110206
ISSN:
0165-2427
Subject:
Porcine epidemic diarrhea virus; immune response; immunopathology; nucleocapsid proteins
Abstract:
... Three commercial ELISAs -two based on spike (E1 and E3) and one on nucleocapsid protein (E2)-were used to analyze the development and persistence of antibodies against Porcine epidemic diarrhea virus (PEDV). Seventy-five four-week-old PEDV-negative piglets were inoculated orally with a European G1b PEDV (INOC) and fourteen were kept as controls (CTRL). After the inoculation, E3 detected positive a ...
DOI:
10.1016/j.vetimm.2021.110206

48. Canine osteosarcoma checkpoint expression correlates with metastasis and T-cell infiltrate

Author:
Matthew J. Cascio; Elizabeth M. Whitley; Bikash Sahay; Galaxia Cortes-Hinojosa; Lung-Ji Chang; Jonathan Cowart; Marc Salute; Elias Sayour; Michael Dark; Zachary Sandoval; Duane A. Mitchell; Rowan J. Milner
Source:
Veterinary immunology and immunopathology 2021 v.232 pp. 110169
ISSN:
0165-2427
Subject:
T-lymphocytes; dogs; humans; immunohistochemistry; immunopathology; ligands; metastasis; osteosarcoma; patients
Abstract:
... Immune-targeted therapies are being successfully implemented into cancer clinical practice. In particular checkpoint inhibitors are employed to modulate the immune microenvironment of solid tumors. We sought to determine the expression of PD-L1, HVEM, and B7H3 in human and canine osteosarcoma, and correlate expression with clinical features and tumor infiltrating lymphocytes in naturally-occurring ...
DOI:
10.1016/j.vetimm.2020.110169

49. Characterization of a variant CD4 molecule in Japanese Black cattle

Author:
Yuko Kato-Mori; Taku Okamura; Norihito Kawashita; Katsuro Hagiwara
Source:
Veterinary immunology and immunopathology 2021 v.232 pp. 110167
ISSN:
0165-2427
Subject:
Wagyu; amino acid substitution; amino acids; cytokines; epitopes; farms; flow cytometry; gene expression; genes; glycoproteins; immunopathology; macrophages; mitogens; monocytes
Abstract:
... Monoclonal antibodies (mAbs) that recognize cluster of differentiation (CD) molecules on lymphocytes are useful tools for the study of different lymphocyte subsets in flow cytometry (FCM) analysis. CD4 is a glycoprotein found on the surfaces of helper T cells, monocytes, macrophages, and dendritic cells. In this study, we describe Japanese Black (JB) calves in a farm whose peripheral blood mononuc ...
DOI:
10.1016/j.vetimm.2020.110167

50. Clinical and immunological responses in sheep after inoculation with Himar1-transformed Anaplasma phagocytophilum and subsequent challenge with a virulent strain of the bacterium

Author:
Sveinung Eskeland; Snorre Stuen; Ulrike G. Munderloh; Anthony Barbet; Liliana Crosby; Kari Lybeck; Peter Wilhelmsson; Per-Eric Lindgren; Shokouh Makvandi-Nejad; Stig Tollefsen; Erik G. Granquist
Source:
Veterinary immunology and immunopathology 2021 v.231 pp. 110165
ISSN:
0165-2427
Subject:
Anaplasma phagocytophilum; acaricides; animal welfare; bacteremia; bacteria; cell lines; disease control; genetic transformation; humoral immunity; immunogenicity; immunopathology; nucleotide sequences; tick-borne diseases; ticks; vaccines; virulence; virulent strains; Norway
Abstract:
... In Norway, the tick-transmitted bacterium Anaplasma phagocytophilum is estimated to cause tick-borne fever (TBF) in 300 000 lambs on pastures each year, resulting in economic and animal welfare consequences. Today, prophylactic measures mainly involve the use of acaricides, but a vaccine has been requested by farmers and veterinarians for decades. Several attempts have been made to produce a vacci ...
DOI:
10.1016/j.vetimm.2020.110165

51. Comparative analysis of the specificity of monoclonal antibodies developed against the bottlenose dolphin, Tursiops truncatus, TNF-α, IL1-β, IL-6, IL-8, IL-10 with monoclonal antibodies made against ovine IFN-γ bovine IL-17A and IL-1β revealed they recognize epitopes conserved on dolphin and bovine orthologues

Author:
Mahmoud M. Elnaggar; Gaber S. Abdellrazeq; Randy E. Sacco; Trevor R. Harsla; Mallory L. Mucci; Lindsay M. Fry; Victoria Hulubei; William C. Davis
Source:
Veterinary immunology and immunopathology 2022 v.250 pp. 110456
ISSN:
0165-2427
Subject:
Tursiops truncatus; cattle; dolphins; epitopes; evolution; immunization; immunopathology; indicator species; interleukin-10; interleukin-17; interleukin-6; interleukin-8; leukocytes; sheep
Abstract:
... Opportunities to include Cetancodontamorpha in the study of the evolution of the immune system in the clades of Artiodactylamorpha, Ruminantiamorpha, Suinamorpha, and Camelidamorpha have increased with the use of the bottlenose dolphin, Tursiops truncatus, as a sentinel species to study the effects of environmental pollutants on the health of marine mammals. Efforts are currently underway to incre ...
Handle:
10113/7803613
DOI:
10.1016/j.vetimm.2022.110456

52. Comparative profiling of the resistance of different genotypes of mannose-binding lectin to Mycoplasma pneumoniae infection in Chinese Merino sheep based on high-throughput sequencing technology

Author:
Mengting Zhu; Ying Nan; Mengting Zhai; Mingyuan Wang; Yanyan Shao; Hugh T. Blair; Stephen Todd Morris; Paul Richard Kenyon; Zongsheng Zhao; Hongmei Zhang
Source:
Veterinary immunology and immunopathology 2021 v.233 pp. 110183
ISSN:
0165-2427
Subject:
GATA transcription factors; Merino; Mycoplasma pneumoniae; blood; gene expression; gene expression regulation; glycoproteins; immunopathology; immunotherapy; innate immunity; lectins; liver; microRNA; sheep
Abstract:
... Mannose-binding lectin (MBL) glycoproteins in blood can selectively recognise lectins on the surface of bacteria, and play an important role in natural immunity. Micro RNAs (miRNAs) are key molecules that regulate gene expression at the post-transcriptional level in vivo, and their pathways are specific and effective. Previous studies indicate that small RNAs such as miRNAs perform regulatory role ...
DOI:
10.1016/j.vetimm.2021.110183

53. Comparison of cellular immune responses to avian influenza virus in two genetically distinct, highly inbred chicken lines

Author:
Emily J. Aston; Ying Wang; Karen E. Tracy; Rodrigo A. Gallardo; Susan J. Lamont; Huaijun Zhou
Source:
Veterinary immunology and immunopathology 2021 v.235 pp. 110233
ISSN:
0165-2427
Subject:
B-lymphocytes; Influenza A virus; T-lymphocytes; antibodies; avian influenza; blood serum; chickens; eggs; flow cytometry; gastrointestinal system; immunopathology; macrophages; nose; pathogenicity; vaccination; viral load; viruses
Abstract:
... Low pathogenicity avian influenza causes mild disease involving the respiratory, gastrointestinal, and reproductive systems of wild and domestic birds. Avian influenza research often emphasizes the effect of the virus genetics on disease, but the influence of host genetics on resistance to infection is not well understood. The genetic determinants of enhanced resistance to influenza can be explore ...
DOI:
10.1016/j.vetimm.2021.110233

54. Comparison of the mucosal adjuvanticities of two Toll-like receptor ligands for recombinant adenovirus-delivered African swine fever virus fusion antigens

Author:
Huipeng Lu; Xiaohui Zhou; Zhi Wu; Xinyu Zhang; Liqi Zhu; Xiaoyu Guo; Quan Zhang; Shanyuan Zhu; Hongfei Zhu; Huaichang Sun
Source:
Veterinary immunology and immunopathology 2021 v.239 pp. 110307
ISSN:
0165-2427
Subject:
Adenoviridae; African swine fever virus; Mycobacterium tuberculosis; Toll-like receptors; Vibrio; antibodies; antibody formation; antigens; flagellin; heat-shock protein 70; immunopathology; interleukin-10; interleukin-4; ligands; lungs; macrophages; mucosal immunity; nose; secondary immunization; swine; vaccination; vaccine adjuvants; vaccine development
Abstract:
... The mucosal immunity plays an important role against African swine fever virus (ASFV) infection and the efficacy of mucosal vaccination is highly dependent on the adjuvant. However, the mucosal adjuvant for ASFV vaccination is poorly studied. Toll-like receptor (TLR) ligands such as the FlaB flagellin from Vibrio vulnificus and the heat shock protein 70 from Mycobacterium tuberculosis (mHsp70) hold ...
DOI:
10.1016/j.vetimm.2021.110307

55. Comparison of the response of mammary gland tissue from two divergent lines of goat with high and low milk somatic cell scores to an experimental Staphylococcus aureus infection

Author:
Rossana Capoferri; Paola Cremonesi; Bianca Castiglioni; Giuliano Pisoni; Paola Roccabianca; Federica Riva; Joel Filipe; Marcello del Corvo; Alessandra Stella; John L. Williams; Rachel Rupp; Paolo Moroni
Source:
Veterinary immunology and immunopathology 2021 v.234 pp. 110208
ISSN:
0165-2427
Subject:
Staphylococcus aureus; chemotaxis; goats; histology; histopathology; immune response; immunopathology; inflammation; interleukin-6; livestock and meat industry; mammary glands; milk; milk quality; neutrophils; somatic cells; transcriptomics; udders
Abstract:
... Mastitis represents one of the major economic and health threats to the livestock sector associated with reduction in milk quality, loss of production and is a major reason for culling. Somatic cell score (SCS) is used as a criterion in breeding programmes to select cows genetically less susceptible to mastitis. The relevance of SCS as a predictor of udder health and susceptibility to mastitis is ...
DOI:
10.1016/j.vetimm.2021.110208

56. Continuous digital hypothermia reduces expression of keratin 17 and 1L-17A inflammatory pathway mediators in equine laminitis induced by hyperinsulinemia

Author:
Lynne Cassimeris; Caitlin Armstrong; Quinnlyn C. Burger; Simon Stokes; Andrew van Eps; Hannah Galantino-Homer
Source:
Veterinary immunology and immunopathology 2021 v.241 pp. 110326
ISSN:
0165-2427
Subject:
ambient temperature; fluorescent antibody technique; horses; human diseases; hyperinsulinemia; hypothermia; immunopathology; inflammation; interleukin-17; interleukin-6; keratin; keratinocytes; laminitis; mice
Abstract:
... The euglycemic hyperinsulinemic clamp model (EHC) of equine endocrinopathic laminitis induces rapid loss of lamellar tissue integrity, disrupts keratinocyte functions, and induces inflammation similar to natural disease. Continuous digital hypothermia (CDH) blocks tissue damage in this experimental model, allowing identification of specific genes or molecular pathways contributing to disease initi ...
DOI:
10.1016/j.vetimm.2021.110326

57. Detection of PRRSV-2 alone and co-localized with CD163 positive macrophages in porcine placental areolae

Author:
Javier Barrera-Zarate; Susan E. Detmer; J. Alex Pasternak; Glenn Hamonic; Daniel J. MacPhee; John C.S. Harding
Source:
Veterinary immunology and immunopathology 2022 v.250 pp. 110457
ISSN:
0165-2427
Subject:
Betaarterivirus suid 2; embryogenesis; endometrium; fetus; fluorescent antibody technique; immunopathology; inflammation; macrophages; phenotype; placenta; pregnancy; transplacental transmission; viral load; viruses
Abstract:
... The porcine epitheliochorial placenta creates a barrier for the transplacental transfer of some nutrients from the dam to the fetus, as well as feto-lethal viruses such as porcine reproductive and respiratory syndrome virus 2 (PRRSV-2). Areolae are specialized structures within the porcine placenta with a high absorptive and substance transport capacity that facilitate embryonic development. The o ...
DOI:
10.1016/j.vetimm.2022.110457

58. Development of A recombinant nucleocapsid based indirect ELISA for the detection of antibodies to avian metapneumovirus subtypes, A, B, and C

Author:
Wanhong Xu; Matthew Suderman; Janice Koziuk; Davor Ojkic; Yohannes Berhane
Source:
Veterinary immunology and immunopathology 2021 v.231 pp. 110151
ISSN:
0165-2427
Subject:
Avian metapneumovirus; Trichoplusia ni; antibodies; antibody formation; antigens; blood serum; chickens; fluorescence; immunopathology; insects; monitoring; nucleocapsid; serodiagnosis
Abstract:
... Nucleocapsid (N) protein is the most highly expressed of all avian metapneumovirus (aMPV) viral proteins and stimulates a substantial immune response in infected animals. Codon optimized recombinant N (rec-N) protein from aMPV subtypes A, B, and C were expressed using the baculoviral expression system in Trichoplusia ni (Tni) insect cells. A mixture of purified rec-N antigens from each subtype was ...
DOI:
10.1016/j.vetimm.2020.110151

59. Development of a cell line-based assay to measure the antibody-dependent cellular cytotoxicity of a canine therapeutic antibody

Author:
Takuya Mizuno; Yoshiho Takeda; Toshihiro Tsukui; Masaya Igase
Source:
Veterinary immunology and immunopathology 2021 v.240 pp. 110315
ISSN:
0165-2427
Subject:
antibodies; cancer therapy; cytotoxicity; dogs; humans; immunopathology; natural killer cells
Abstract:
... Antibody drugs are one of the most important therapeutic modalities in cancer treatment. Antibody drugs for canine cytotoxicity have been developed, and the most important way to evaluate the function of antibody drugs in vitro is to measure the antibody-dependent cellular cytotoxicity (ADCC) activity. However, a good method for measuring the ADCC activity of antibody drugs for dogs has not yet be ...
DOI:
10.1016/j.vetimm.2021.110315

60. Diagnostic accuracy of digital Brix and serum total protein refractometers in estimating different passive immunity levels in dairy calves

Author:
Mehmet Akköse; Hüseyin Gökhan Kutsal; Mert Kurban; Elif Merve Çinar; Yadigar Polat; Murat Cengiz
Source:
Veterinary immunology and immunopathology 2022 v.249 pp. 110442
ISSN:
0165-2427
Subject:
Holstein; blood serum; brix; immunopathology; passive immunity; refractive index
Abstract:
... The aim of this study was to determine the diagnostic accuracy of digital serum total protein (TP) and digital Brix refractometers in estimating different passive immunity levels (<10, <18, <25 mg/mL) in dairy calves. Blood samples were collected from 260 apparently healthy Holstein calves, aged 2–7 days. Serum IgG concentrations were measured using digital Brix and TP refractometers and the radia ...
DOI:
10.1016/j.vetimm.2022.110442

61. Differential gene expression and Ingenuity Pathway Analysis of bronchoalveolar lavage cells from horses with mild/moderate neutrophilic or mastocytic inflammation on BAL cytology

Author:
Kaori Uchiumi Davis; M. Katie Sheats
Source:
Veterinary immunology and immunopathology 2021 v.234 pp. 110195
ISSN:
0165-2427
Subject:
asthma; cell biology; chemokines; descriptive studies; disease severity; etiology; fibrosis; gene expression; gene expression regulation; horses; immunoassays; immunopathology; inflammation; pathophysiology; sequence analysis; transcriptome
Abstract:
... Mild to moderate equine asthma syndrome (mEAS) affects horses of all ages and breeds. To date, the etiology and pathophysiology of mEAS are active areas of research, and it remains incompletely understood whether mEAS horses with different immune cell ‘signatures’ on BAL cytology represent different phenotypes, distinct pathobiological mechanisms (endotypes), varied environmental conditions, disea ...
DOI:
10.1016/j.vetimm.2021.110195

62. Distribution of M1 and M2 macrophages in cerebral granulomas caused by Encephalitozoon cuniculi

Author:
Adolfo Soto-Domínguez; Jennifer A. González-Castillo; Uziel Castillo-Velázquez; Luis E. Rodriguez-Tovar; Gerardo Méndez-Zamora; Diana E. Zamora-Avila; Alicia M. Nevárez-Garza
Source:
Veterinary immunology and immunopathology 2022 v.252 pp. 110481
ISSN:
0165-2427
Subject:
Encephalitozoon cuniculi; brain; granuloma; immunology; immunopathology; inflammation; macrophages; phenotype
Abstract:
... Encephalitozoon cuniculi spores cause severe granulomatous inflammation in the brain where mononuclear cells and macrophages infiltrate. Here, we orally infected New Zealand white rabbits with 1 × 10⁶E. cuniculi viable spores to study the recruitment and localization of macrophages in brain granulomas. At day 30 post-infection, the positive phenotype markers iNOS (M1) and Arg-1 (M2) were located i ...
DOI:
10.1016/j.vetimm.2022.110481

63. Dynamics of local gene regulations in synovial fluid leukocytes from horses with lipopolysaccharide-induced arthritis

Author:
Marie Walters; Kerstin Skovgaard; Pia Haubro Andersen; Peter M.H. Heegaard; Stine Jacobsen
Source:
Veterinary immunology and immunopathology 2021 v.241 pp. 110325
ISSN:
0165-2427
Subject:
antagonists; arthritis; cartilage; chemokines; chondrocytes; gene expression; genes; immunopathology; inflammation; interleukin-10; interleukin-6; lipopolysaccharides; models; neutrophil collagenase; prostaglandin synthase; superoxide dismutase; synovial fluid; tumor necrosis factors
Abstract:
... The role of resident cells such a synoviocytes and chondrocytes in intra-articular inflammation is well-characterized, however the in vivo gene expression patterns of cells (predominantly leukocytes) in the synovial fluid (SF) of an inflamed joint have never previously been investigated. The aim of this study was to investigate gene expression in SF leukocytes from the inflamed joint cavity after ...
DOI:
10.1016/j.vetimm.2021.110325

64. Effect of Holstein genotype on ex-vivo cytokine response to lipopolysaccharide (LPS) and lipoteichoic acid (LTA) during the periparturient period

Author:
Amber A. Brink; Wanda J. Weber; John D. Lippolis; John B. Cole; Sandra M. Godden; Anthony Seykora; Brian A. Crooker
Source:
Veterinary immunology and immunopathology 2022 v.251 pp. 110463
ISSN:
0165-2427
Subject:
Holstein; energy balance; genotype; immunopathology; innate immunity; interleukin-6; lactation; lipopolysaccharides; lipoteichoic acids; milk; pathogen-associated molecular patterns
Abstract:
... Effects of Holstein genotype on innate immune response were assessed with ex-vivo lipopolysaccharide (LPS) and lipoteichoic acid (LTA) stimulation of whole blood from unselected (UH, n = 10) and contemporary (CH, n = 11) Holsteins that differ in production by more than 4,500 kg/lactation. Blood was collected at −14, 7, 28, and 49 days in milk (DIM), mixed with a pathogen-associated molecular patte ...
Handle:
10113/7837475
DOI:
10.1016/j.vetimm.2022.110463

65. Effect of IL-8 haplotype on temporal profile in circulating concentrations of interleukin 8 and 25(OH) vitamin D in Holstein-Friesian calves

Author:
Megan B. O’Brien; Rachel M. McLoughlin; Colm Roche; Corwin D. Nelson; Kieran G. Meade
Source:
Veterinary immunology and immunopathology 2021 v.238 pp. 110287
ISSN:
0165-2427
Subject:
Holstein; blood serum; disease susceptibility; genes; haplotypes; immune response; immunopathology; innate immunity; interleukin-8; monocytes; neutrophils; temporal variation; vitamin D
Abstract:
... Interleukin-8 (IL-8) is an inflammatory chemokine released during the primary innate immune response to recruit neutrophils to the site of infection. Two distinct gene promoter haplotypes have been previously reported to segregate in the Holstein-Friesian breed (IL8-h1 and IL8-h2). Our earlier work showed how these divergent IL8 haplotypes influence IL-8 concentration and other immune response par ...
DOI:
10.1016/j.vetimm.2021.110287

66. Effect of IL8 haplotype on immunological traits in periparturient dairy cows

Author:
Giovanna De Matteis; Maria Carmela Scatà; Francesco Grandoni; Alessandra Crisà; Megan B. O’Brien; Kieran G. Meade; Gennaro Catillo
Source:
Veterinary immunology and immunopathology 2021 v.238 pp. 110288
ISSN:
0165-2427
Subject:
CD4-positive T-lymphocytes; Holstein; Simmental; amyloid; blood serum; cytotoxicity; early lactation; genes; genotyping; haplotypes; haptoglobins; homozygosity; immune response; immunopathology; innate immunity; interleukin-8; monocytes; reactive oxygen species
Abstract:
... Interleukin 8 (IL8) is a major mediator of the innate immune response. Polymorphisms in this gene are associated with susceptibility to inflammatory disease in humans. Two major promoter polymorphic haplotypes (IL8-h1 and IL8-h2) segregating in cattle populations have shown a significant effect on the immune response profile in calves but their implications for transition cow immunity have not bee ...
DOI:
10.1016/j.vetimm.2021.110288

67. Effect of In-vivo heat challenge on physiological parameters and function of peripheral blood mononuclear cells in immune phenotyped dairy cattle

Author:
S.L. Cartwright; J. Schmied; A. Livernois; B.A. Mallard
Source:
Veterinary immunology and immunopathology 2022 v.246 pp. 110405
ISSN:
0165-2427
Subject:
barns; body temperature; cell proliferation; cell respiration; climate change; dairy cattle; disease incidence; heat; heat stress; heat tolerance; heat-shock protein 70; humidity; immunology; immunopathology; vaccines
Abstract:
... The frequency of heat waves and hot days are increasing due to climate change, which leads to an increase in the occurrence of heat stress in dairy cattle. Previous studies have shown that dairy cattle identified as high immune responders have a reduced incidence of disease and improved vaccine response compared to average and low responders. Additionally, it has been observed that when cells from ...
DOI:
10.1016/j.vetimm.2022.110405

68. Effect of polymyxin B on ex vivo tumor necrosis factor-alpha responsiveness of blood leukocytes in Danish Holstein Friesian cows

Author:
Momena Khatun; Birthe M. Damgaard; Jens B. Andersen; Christine M. Røntved
Source:
Veterinary immunology and immunopathology 2021 v.238 pp. 110293
ISSN:
0165-2427
Subject:
Escherichia coli; Holstein; Staphylococcus aureus; Streptococcus dysgalactiae; Streptococcus uberis; cell walls; early lactation; immunopathology; milk; peptidoglycans; polymyxin B; tumor necrosis factor-alpha; udders
Abstract:
... Whole blood stimulation assay (WBA) with killed gram-positive and gram-negative udder pathogens were used to investigate the interference of the endotoxin-binding antibiotic polymyxin B (PMB) on the ex vivo TNF-α response. Blood samples were collected from first to third lactating dairy cows in their early lactation (<50 days in milk, n = 32) period. The WBA was stimulated with both inactivated ba ...
DOI:
10.1016/j.vetimm.2021.110293

69. Effect of quercetin on formation of porcine neutrophil extracellular trap

Author:
Hyun-Min Jo; Changhwan Ahn; Hakhyun Kim; Byeong-Teck Kang; Eui-Bae Jeung; Mhan-Pyo Yang
Source:
Veterinary immunology and immunopathology 2021 v.241 pp. 110335
ISSN:
0165-2427
Subject:
anti-inflammatory activity; dyes; gene expression; immune response; immunopathology; lipopolysaccharides; neutrophils; propidium; quercetin; swine
Abstract:
... Neutrophil extracellular trap (NET) formation is an immune response to the invasion of external microorganisms. Quercetin, a member of the flavonoid family found in fruits and vegetables, has been examined in multiple biological contexts. The objective of this study was to examine the effect of quercetin on porcine NET formation. We measured NET formation by peripheral blood polymorphonuclear cell ...
DOI:
10.1016/j.vetimm.2021.110335

70. Effects of continuous passage on the immunomodulatory properties of equine bone marrow-derived mesenchymal stem cells in vitro

Author:
Shannon S. Connard; Renata L. Linardi; Kayla M. Even; Alix K. Berglund; Lauren V. Schnabel; Kyla F. Ortved
Source:
Veterinary immunology and immunopathology 2021 v.234 pp. 110203
ISSN:
0165-2427
Subject:
T-lymphocytes; bone marrow; cell proliferation; cell structures; chemokine CCL2; chemokine CCL5; horses; immunomodulators; immunopathology; immunophenotype; interferon-gamma; interleukin-10; interleukin-6; interleukin-8; tissue repair; tumor necrosis factor-alpha
Abstract:
... The immunomodulatory properties of mesenchymal stem cells (MSCs) have been studied extensively due to their increasing clinical application for tissue regeneration and repair following culture expansion. We have studied the effect of continuous passage on the immunomodulatory capacity of equine bone marrow-derived MSCs (BM-MSCs). Equine BM-MSCs were isolated and culture expanded to passage three, ...
DOI:
10.1016/j.vetimm.2021.110203

71. Effects of frequent teat stimulation on antimicrobial component production in mammary glands of lactating goats

Author:
Yusaku Tsugami; Rika Harada; Takahiro Nii; Naoki Suzuki; Naoki Isobe
Source:
Veterinary immunology and immunopathology 2022 v.249 pp. 110431
ISSN:
0165-2427
Subject:
Japan; calcium-binding proteins; epithelium; humans; immunopathology; keratinocytes; lactoferrin; milk; milk yield
Abstract:
... Various antimicrobial components, such as lactoferrin, S100 calcium-binding protein A7 (S100A7), and IgA, produced by epithelial cells and leukocytes in lactating mammary glands are important for host defense against invading pathogens. Increase in milking frequency enhances milk yield in ruminants and implies an increase in frequency of teat stimulation. However, the influence of frequent teat st ...
DOI:
10.1016/j.vetimm.2022.110431

72. Establishing a reference interval for acute phase proteins, cytokines, antioxidants and commonly measured biochemical and hematologic parameters in the northern fur seal (Callorhinus ursinus)

Author:
Valerie Johnson; A. Russell Moore; Rachel Conway; Tonya Zeppelin; Tom Gelatt; Colleen Duncan
Source:
Veterinary immunology and immunopathology 2021 v.242 pp. 110348
ISSN:
0165-2427
Subject:
C-reactive protein; Callorhinus ursinus; adults; biomarkers; blood serum; dogs; electrophoresis; females; fur; hydrogen peroxide; immunopathology; inflammation; interleukin-10; interleukin-6; interleukin-8; malondialdehyde; oxidative stress; selenium; vitamin E; Bering Sea
Abstract:
... Over the past several decades there has been a precipitous decline of northern fur seals (Callorhinus ursinus; NFS) at their breeding grounds on the Pribilof Islands in the Bering Sea. The cause of this decline is likely multifactorial and could include changes in environmental parameters, prey abundance and distribution as well as exposure to pathogens and pollutants. Evaluation of inflammatory m ...
DOI:
10.1016/j.vetimm.2021.110348

73. Evaluating the Immunogenicity of Recombinant VP1 Protein from the Foot-and-Mouth Disease Virus Encapsulated in Nanoliposome in Guinea Pig Animal Model

Author:
Marjan Kazemi; Mahmoud Reza Aghamaali; Rasool Madani; Tara Emami; Fariba Golchinfar
Source:
Veterinary immunology and immunopathology 2022 pp. 110497
ISSN:
0165-2427
Subject:
Foot-and-mouth disease virus; adjuvants; animal models; blood serum; cholesterol; foot-and-mouth disease; genes; guinea pigs; immune response; immunogenicity; immunopathology; livestock; neutralization; viruses
Abstract:
... Foot-and-mouth disease (FMD) is considered as contagious in livestock, which is caused by the Picornavridae virus family known as FMD virus (FMDV). In the present study, the VP1 gene from FMDV (O strain) was expressed and purified. In addition, nanoliposomes were utilized as an adjuvant. After formulating the nanoliposomes with DMPC, DMPG, and cholesterol, the recombinant VP1 protein was encapsula ...
DOI:
10.1016/j.vetimm.2022.110497

74. Evaluation of PD-1 and PD-L1 expression in canine urothelial carcinoma cell lines

Author:
Christopher J. Pinard; Samuel E. Hocker; Andrew C. Poon; Jordon M. Inkol; Arata Matsuyama; R. Darren Wood; Geoffrey A. Wood; J. Paul Woods; Anthony J. Mutsaers
Source:
Veterinary immunology and immunopathology 2022 v.243 pp. 110367
ISSN:
0165-2427
Subject:
Western blotting; carcinoma; cell lines; dogs; flow cytometry; fluorescence microscopy; gene expression; humans; immunopathology; immunotherapy; neoplasm cells; reverse transcriptase polymerase chain reaction
Abstract:
... Urothelial carcinoma (UC) is the most common urinary tumor in dogs and despite combinational therapies, only modest improvements in survival have been achieved in recent years. Given the utility of monoclonal antibodies against PD-1 and PD-L1 in human UC, we evaluated the protein and mRNA expression in three established canine urothelial carcinoma cell lines. Flow cytometry and western blot analys ...
DOI:
10.1016/j.vetimm.2021.110367

75. Evaluation of candidate reference genes for quantitative RTqPCR analysis in goldfish (Carassius auratus L.) in healthy and CyHV-2 infected fish

Author:
Arathi Dharmaratnam; Arun Sudhagar; Sundar Raj Nithianantham; Sweta Das; Thangaraj Raja Swaminathan
Source:
Veterinary immunology and immunopathology 2021 v.237 pp. 110270
ISSN:
0165-2427
Subject:
Carassius auratus; algorithms; disease control; gene expression; goldfish; heart; immunology; immunopathology; intestines; kidneys; liver; muscles; pathogens; peptide elongation factors; quantitative polymerase chain reaction; ribosomal RNA; spleen; world wide web
Abstract:
... The accuracy of quantitative real time PCR (RTqPCR) can be attained only when a suitable reference gene is used. The gene expression for a particular gene may vary within different cells at different conditions. Hence, the suitability and stability of various potential reference genes have to be determined for expression studies. In this study, we have examined the potential of four different refe ...
DOI:
10.1016/j.vetimm.2021.110270

76. Evaluation of equine xenogeneic mixed lymphocyte reactions using 5-ethynyl-2’-deoxyuridine (EdU)

Author:
Kate E. Birdwhistell; David J. Hurley; Bradley Heins; John F. Peroni
Source:
Veterinary immunology and immunopathology 2022 v.249 pp. 110430
ISSN:
0165-2427
Subject:
T-lymphocytes; cell proliferation; cytokines; flow cytometry; horses; immunopathology; nucleobases; organ transplantation; swine; thymidine
Abstract:
... Allogeneic solid organ transplantation is currently the only treatment option for end stage organ disease. The shortage of available donor organs has driven efforts to utilize xenogeneic organs for transplantation. In vitro methods for evaluating immune-compatibility are a quick and low cost means of screening novel tissue products prior to more involved, expensive, and invasive live animal studie ...
DOI:
10.1016/j.vetimm.2022.110430

77. Evaluation of immunogenicity and protection mediated by Lawsonia intracellularis subunit vaccines

Author:
Kezia R. Fourie; Pooja Choudhary; Siew Hon Ng; Milan Obradovic; Robert Brownlie; Sanjeev K. Anand; Heather L. Wilson
Source:
Veterinary immunology and immunopathology 2021 v.237 pp. 110256
ISSN:
0165-2427
Subject:
Lawsonia intracellularis; adjuvants; bacteria; blood serum; dipeptides; feces; flagellum; glycoproteins; humoral immunity; ileum; immunogenicity; immunopathology; intestinal mucosa; jejunum; methionine adenosyltransferase; monitoring; polyinosinic-polycytidylic acid; proteinases; proteolysis; quantitative polymerase chain reaction; stress response; subunit vaccines; viral antigens
Abstract:
... Lawsonia intracellularis is an economically important bacterium that causes ileitis in pigs. Current vaccines for L. intracellularis do not allow for differentiation between infected and vaccinated animals (DIVA), which is beneficial for disease tracking and surveillance. Previously, we identified five putative surface L. intracellularis proteins that were targeted by antibodies from pigs infected ...
DOI:
10.1016/j.vetimm.2021.110256

78. Ex vivo activated CD4+ T cells from young calves exhibit Th2-biased effector function with distinct metabolic reprogramming compared to adult cows

Author:
Jordan M. Eder; Randy E. Sacco
Source:
Veterinary immunology and immunopathology 2022 v.248 pp. 110418
ISSN:
0165-2427
Subject:
acidification; adults; gene expression; glycolysis; immunopathology; interleukin-4; mitochondria; oxygen consumption; passive immunity; phenotype
Abstract:
... As maternal passive immunity wanes at 6–8 weeks, young calves must rely on their own naïve and developing immune system for protection against pathogens. Typically, an infection in the young induces a T cell-mediated response, which skews towards a Th2 phenotype and results in a reduced effector response. Our study examines the implications this transitional period of immunocompetency has on cellu ...
DOI:
10.1016/j.vetimm.2022.110418

79. Ex vivo tumor necrosis factor-alpha response of blood leukocytes in Danish Holstein-Friesian cows stimulated by Gram-positive and Gram-negative bacteria isolated from mastitic milk

Author:
Momena Khatun; Birthe M. Damgaard; Jens B. Andersen; Christine M. Røntved
Source:
Veterinary immunology and immunopathology 2021 v.234 pp. 110204
ISSN:
0165-2427
Subject:
Escherichia coli; Holstein; Staphylococcus aureus; Streptococcus dysgalactiae; Streptococcus uberis; endotoxins; humans; immunopathology; lactation; mastitis; milk; peptidoglycans; polymyxin B; tumor necrosis factor-alpha; udders
Abstract:
... A whole blood stimulation assay was used to investigate the effects of parity, number of weeks after calving and Gram-positive and Gram-negative bacteria on the ex vivo TNF-α responsiveness of Danish Holstein-Friesian cows of first to third lactation (n = 28). Blood samples were collected in weeks 2, 3, 5 and 8 after parturition and stimulated with Escherichia coli LPS (10 μg/mL), Staphylococcus a ...
DOI:
10.1016/j.vetimm.2021.110204

80. Exonic SNP in MHC-DMB2 is associated with gene expression and humoral immunity in Japanese quails

Author:
Hassan Chenani; Mahmood Nazari; Mohamad Taghi Beigi Nassiri; Hedaiatollah Roshanfekr; Ali Aghaie
Source:
Veterinary immunology and immunopathology 2021 v.239 pp. 110302
ISSN:
0165-2427
Subject:
DNA; DNA fragmentation; antibody formation; birds; breast muscle; computer simulation; erythrocytes; exons; gene expression; genotype; humoral immunity; immunopathology; protein structure; sheep; single nucleotide polymorphism
Abstract:
... The DMB2 gene is widely expressed at high levels in avian. This gene plays an important role in humoral immunity. The aim of this study was to investigate the effects of 361 G > C Single nucleotide polymorphism (SNP) on DMB2 protein structure and gene expression to determine how the 361 G > C SNP affects humoral immune response in Japanese quails. 0.2 mL of 5% sheep red blood cell (SRBC) was injec ...
DOI:
10.1016/j.vetimm.2021.110302

81. Feline lymphoplasmacytic rhinitis (FLPCR): Severity of inflammation correlates with reduced mucosal IgA expression

Author:
Paola Roccabianca; Sara Turchetto; Nicola Ferrari; Claudia Romeo; Serena Bonacini; Carlo Maria Mortellaro; Francesco Godizzi; Marco Tecilla
Source:
Veterinary immunology and immunopathology 2021 v.234 pp. 110193
ISSN:
0165-2427
Subject:
angiogenesis; cats; cell membranes; females; fibrosis; immunohistochemistry; immunopathology; inflammation; mucins; neutrophils; pathogenesis; rhinitis; serology
Abstract:
... Feline lymphoplasmacytic rhinitis (FLPCR) is a rare disease with an unclear pathogenesis characterized by lymphoplasmacytic (LPC) inflammation and progressive tissue destruction. Aims were to evaluate specific FLPCR clinical and pathological features to gain insights into disease pathogenesis. Signalment, clinical signs, serology and 47 pin. h biopsies were retrospectively collected from 33 FLPCR ...
DOI:
10.1016/j.vetimm.2021.110193

82. Field evaluation of the tuberculin skin test for the detection of Mycobacterium tuberculosis complex infection in communal goats (Capra hircus) in KwaZulu-Natal, South Africa

Author:
Deborah M. Cooke; Wynand J. Goosen; Carmel Witte; Michele A. Miller
Source:
Veterinary immunology and immunopathology 2022 v.252 pp. 110486
ISSN:
0165-2427
Subject:
Capra hircus; Mycobacterium tuberculosis complex; Saanen; Syncerus caffer; cattle; dairy herds; goats; immunopathology; land reform; pathogens; risk; rural development; tuberculin; tuberculosis; South Africa
Abstract:
... In South Africa, animal tuberculosis (TB) control programs predominantly focus on domestic cattle and African buffaloes (Syncerus caffer) despite increasing global reports of tuberculosis in goats (Capra hircus). Left undetected, Mycobacterium tuberculosis complex (MTBC) infected goats may hinder TB eradication efforts in cattle and increase zoonotic risk to humans. Since the publication of animal ...
DOI:
10.1016/j.vetimm.2022.110486

83. HSP110 expression in canine mammary gland tumor and its correlation with histopathological classification and grade

Author:
Satoru Okada; Masaru Furuya; Ayano Fukui-Kaneshige; Hitoshi Nakanishi; Hiroyuki Tani; Kazumi Sasai
Source:
Veterinary immunology and immunopathology 2021 v.232 pp. 110171
ISSN:
0165-2427
Subject:
adenoma; blood serum; carcinogenesis; carcinoma; dogs; heat stress; histopathology; homeostasis; humans; immunology; immunopathology; mammary glands
Abstract:
... Heat shock proteins (HSPs) play critical roles as molecular chaperones, thereby promoting cellular homeostasis. HSPs are overexpressed in many types of human tumors and their serum concentration is elevated in cancer patients. Recent studies have suggested that HSPs may promote tumorigenesis via interactions with tumor-related proteins. There are only a few studies that address the expression of H ...
DOI:
10.1016/j.vetimm.2020.110171

84. Haemonchus contortus infection induces a variable immune response in resistant and susceptible Pelibuey sheep

Author:
Alvar Alonzo Cruz-Tamayo; Ma. Eugenia López-Arellano; Roberto González-Garduño; Glafiro Torres-Hernández; Alfonso de la Mora-Valle; Carlos Becerril-Pérez; Omar Hernández-Mendo; Efrén Ramírez-Bribiesca; Miguel Huchin-Cab
Source:
Veterinary immunology and immunopathology 2021 v.234 pp. 110218
ISSN:
0165-2427
Subject:
Haemonchus contortus; Pelibuey (sheep breed); body weight; gene expression; granuloma; hemoglobin; histopathology; immune response; immunoglobulin A; immunopathology; interleukin-5; interleukin-8; leukocytes; males; phenotype; protein content
Abstract:
... The immune response and phenotypic characteristics of Pelibuey lambs were analysed after the induction of a Haemonchus contortus trickle infection. Male lambs (n = 29; 20 kg live weight) were infected with 100 H. contortus infective larvae per kg of live weight on day 3, 5 and 7 of the experiment. The number of eggs per gram (epg), seven haematological parameters and the immunoglobulin A (IgA) lev ...
DOI:
10.1016/j.vetimm.2021.110218

85. Heterogeneous populations from in vitro cultures of antigen presenting cells in pigs

Author:
Myriam Pujol; Efrain Guzman; Sergio Montaner-Tarbes; Maria Montoya
Source:
Veterinary immunology and immunopathology 2021 v.234 pp. 110215
ISSN:
0165-2427
Subject:
antigens; bone marrow; immunopathology; macrophages; monocytes; phenotype; swine
Abstract:
... Dendritic cells (DCs) are the most potent antigen presenting cells (APCs). Because of the difficulty in obtaining these cells directly from tissues, different sources of DCs are frequently used for in vitro experimentation and many of their biological and functional characteristics were studied using these systems. Until recently, it was assumed that specific culture conditions polarized the diffe ...
DOI:
10.1016/j.vetimm.2021.110215

86. Histamine triggers the formation of neutrophil extracellular traps via NADPH oxidase, ERK and p38 pathways

Author:
Ershun Zhou; Zhikai Wu; Xingyi Zhu; Peixuan Li; Jingjing Wang; Zhengtao Yang
Source:
Veterinary immunology and immunopathology 2021 v.235 pp. 110234
ISSN:
0165-2427
Subject:
NAD (coenzyme); NAD(P)H oxidase (H2O2-forming); allergic rhinitis; asthma; cattle; confocal microscopy; dose response; histamine; immunology; immunopathology; lactate dehydrogenase; mitogen-activated protein kinase; neutrophils; reactive oxygen species; viability
Abstract:
... Histamine plays a central role in various allergic diseases, such as allergic asthma and allergic rhinitis. Neutrophil extracellular traps (NETs) formation is a novel effector mechanism of neutrophils to defend against various stimuli. In this present study, we aimed to investigate the role of histamine on bovine NET formation, and examined its preliminary molecular mechanisms. Cell Counting Kit-8 ...
DOI:
10.1016/j.vetimm.2021.110234

87. Identification of EDIL3 biomarkers as a biomarker and potential therapeutic target of canine mammary carcinomas based on integrated bioinformatics analysis

Author:
Zixiang Lin; Ce Jiang; Di Lv; Degui Lin
Source:
Veterinary immunology and immunopathology 2022 v.249 pp. 110432
ISSN:
0165-2427
Subject:
bioinformatics; biomarkers; carcinogenesis; data collection; dogs; genes; immunity; immunopathology; mammary neoplasms (animal); therapeutics
Abstract:
... As the fierce battle with cancer is now expanding to companion animals, effective treatment of canine mammary carcinomas (CMT), as the most frequently diagnosed tumor in intact dogs, is becoming a crucial issue. Although many studies have been carried out concerning the clinical application of mammary tumor biomarkers, no ideal biomarker has yet been identified in CMT. Therefore, in this work, we ...
DOI:
10.1016/j.vetimm.2022.110432

88. Immune responses induced by inactivated porcine reproductive and respiratory syndrome virus (PRRSV) vaccine in neonatal pigs using different adjuvants

Author:
Sandra Vreman; Norbert Stockhofe-Zurwieden; Ditta J. Popma-de Graaf; Huub F.J. Savelkoul; C. Barnier-Quer; N. Collin; Damien Collins; Dennis McDaid; Anne C. Moore; Johanna M.J. Rebel
Source:
Veterinary immunology and immunopathology 2021 v.232 pp. 110170
ISSN:
0165-2427
Subject:
T-lymphocytes; antibody formation; emulsions; immunogenicity; immunopathology; lungs; lymph; necropsy; pathogens; porcine reproductive and respiratory syndrome; secondary immunization; squalene; vaccination; vaccines; viremia; viruses
Abstract:
... Vaccination of neonatal pigs could be supportive to prevent porcine reproductive and respiratory syndrome virus (PRRSV), which is an important porcine pathogen causing worldwide welfare and health problems in pigs of different age classes. However, neonatal immunity substantially differs to adults, thus different vaccines may be required in neonateal pigs. We examined if the immunogenicity and eff ...
DOI:
10.1016/j.vetimm.2020.110170

89. Increased T-cell responses that control bovine leukemia virus proviral load in beef cattle under dietary vitamin A restriction for marbling

Author:
Sonoko Miyauchi; Yuzuru Katagiri; Chihiro Ochiai; Toh-ichi Hirata; Keiichi Matsuda; Toshihiro Ichijo; Hirokazu Hikono; Kenji Murakami
Source:
Veterinary immunology and immunopathology 2021 v.239 pp. 110301
ISSN:
0165-2427
Subject:
Bovine leukemia virus; Wagyu; beef cattle; diet; gene expression; immunopathology; marbling; vitamin A
Abstract:
... Bovine leukemia virus (BLV) proviral load is controlled by T-cell responses, which require vitamin A (VA) derived from food. However, whether dietary VA restriction for marbling impairs the T-cell responses that control BLV proviral load in beef cattle is unknown. We assessed T-cell subsets, interferon (IFN)-γ gene expression, and BLV proviral load in naturally BLV-infected Japanese Black cattle t ...
DOI:
10.1016/j.vetimm.2021.110301

90. Interferon gamma protective against Sarcocystis neurona encephalitis in susceptible murine model

Author:
Alayna N. Hay; Ashley Potter; David Lindsay; Tanya LeRoith; Jing Zhu; Sarah Cashwell; Sharon Witonsky; Caroline Leeth
Source:
Veterinary immunology and immunopathology 2021 v.240 pp. 110319
ISSN:
0165-2427
Subject:
Protozoa; Sarcocystis neurona; T-lymphocytes; animal models; cerebellum; cytokines; disease resistance; disease susceptibility; equine protozoal myeloencephalitis; etiological agents; histology; horses; immune response; immunopathology; interferon-gamma; mice; protective effect
Abstract:
... Sarcocystis neurona is the predominant etiological agent of the infectious equine neurologic disease, equine protozoal myeloencephalitis (EPM), which is prevalent in the United States. A wealth of knowledge about S. neurona biology and its life cycle has accumulated over the last several decades. However, much remains unknown about the aberrant equine host’s immune response to S. neurona and the r ...
DOI:
10.1016/j.vetimm.2021.110319

91. Interferon gamma, lipopolysaccharide, and modified-live viral vaccines stimulation alter the mRNA expression of tumor necrosis factor α, inducible nitric oxide synthase, and interferon β in bovine alveolar macrophages

Author:
Shingo Ishikawa; Masataka Miyazawa; Chiho Tanaka; Ryoma Uesawa; Juri Nishizawa; Ryoko Uemura; Ikuo Kobayashi; Seiji Hobo
Source:
Veterinary immunology and immunopathology 2022 v.244 pp. 110378
ISSN:
0165-2427
Subject:
bovine respiratory disease; cattle; gene expression; immunopathology; inducible nitric oxide synthase; interferon-gamma; lipopolysaccharides; macrophages; pathogenesis; polyinosinic-polycytidylic acid; sodium; therapeutics; tumor necrosis factor-alpha
Abstract:
... To understand the pathogenesis of bovine respiratory disease (BRD), it is necessary to elucidate the mechanisms of alveolar macrophage regulation by cytokines and pathogen-associated molecular patterns (PAMPs). Moreover, "non-specific effects (NSEs)” an innate immune regulatory mechanism in response to vaccines containing PAMPs, has recently attracted attention. It may be applied to BRD control, b ...
DOI:
10.1016/j.vetimm.2021.110378

92. Intramammary infection caused by Staphylococcus aureus increases IgA antibodies to iron-regulated surface determinant-A, -B, and -H in bovine milk

Author:
Yuya Nagasawa; Ikuo Uchida; Fuyuko Tanabe; Aya Hirose; Kazue Sugawara; Yoshio Kiku; Takehito Iwata; Chieko Kato; Yusuke Yamashita; Tomohito Hayashi
Source:
Veterinary immunology and immunopathology 2021 v.235 pp. 110235
ISSN:
0165-2427
Subject:
Staphylococcus aureus; absorbance; antigens; bacteriophages; bovine mastitis; gametolysin; glycerol; humoral immunity; imidazole; immunogenicity; immunopathology; milk; phosphates; vaccination; virulence
Abstract:
... The aim of this study was to identify virulence factors that have high immunogenicity. An in vivo-expressed Staphylococcus aureus antigen was identified by probing bacteriophage expression libraries of S. aureus with antibodies in bovine mastitis milk. Eighteen clones were isolated, and their proteins were identified as 5 characterised proteins (IsdA, Protein A, IsdB, autolysin, and imidazole glyc ...
DOI:
10.1016/j.vetimm.2021.110235

93. Investigation of innate immune function in adult and geriatric horses

Author:
Ashton B. Miller; Alan T. Loynachan; Virginia D. Barker; Amanda A. Adams
Source:
Veterinary immunology and immunopathology 2021 v.235 pp. 110207
ISSN:
0165-2427
Subject:
Escherichia coli; Rhodococcus equi; adults; body weight; gene expression; horses; immune response; immunopathology; insulin; interleukin-10; interleukin-13; interleukin-6; interleukin-8; monocytes; myeloperoxidase; neutrophils
Abstract:
... In order to better understand the influence of age on innate immune function in horses, blood was collected from twelve adult horses (aged 10–16 years; mean: 13 years) and ten geriatric horses (aged 18–26 years; mean: 21.7 years) for analysis of plasma myeloperoxidase, complete blood counts, and cytokine and receptor expression in response to in vitro stimulation with heat-inactivated Rhodococcus ...
DOI:
10.1016/j.vetimm.2021.110207

94. Linear polysaccharides reduce production of inflammatory cytokines by LPS-stimulated bovine fibroblasts

Author:
Brian M. Kobylkevich; M. Jahir Raihan; Tirth Uprety; Radhey S. Kaushik; Jay S. Shore; Joel J. Sohn; Mark A. Messerli
Source:
Veterinary immunology and immunopathology 2021 v.234 pp. 110220
ISSN:
0165-2427
Subject:
animal care; biodegradability; cattle; cell viability; cellulose; chitin; chitosan; cytokines; cytotoxicity; farms; fibroblasts; immunopathology; inflammation; lameness; lipopolysaccharides; oxidation
Abstract:
... Chronic lesions in the limbs of farm animals cause lameness due to chronic infection and inflammation. Exploratory treatments for chronic wounds in humans may be suitable for adaptation into the field of animal care. Specifically, antimicrobial linear polysaccharides like oxidized regenerated cellulose (ORC) and chitin/chitosan are biodegradable hemostats that are being explored to promote healing ...
DOI:
10.1016/j.vetimm.2021.110220

95. Lipopolysaccharide (LPS) induced inflammatory changes to differentially expressed miRNAs of the host inflammatory response

Author:
Trevon Swain; Christine M. Deaver; Anne Lewandowski; Michael J. Myers
Source:
Veterinary immunology and immunopathology 2021 v.237 pp. 110267
ISSN:
0165-2427
Subject:
Escherichia coli; biomarkers; body weight; endotoxemia; immunology; immunopathology; inflammation; lipopolysaccharides; microRNA; quantitative polymerase chain reaction; swine
Abstract:
... In veterinary medicine, inflammation in swine is evaluated principally by clinical signs. This method is often unreliable when assessing large animal populations because of inconsistent interpretations of clinical observations. This study examined whether changes in miRNA expression can predict the severity of the inflammatory response in swine after administration of Escherichia coli lipopolysacc ...
DOI:
10.1016/j.vetimm.2021.110267

96. Low molecular weight heparin reduces acute phase response and multiple organ dysfunction following Ovine experimental endotoxemia model

Author:
Rouhollah Esmaeili Seraji; Aliasghar Chalmeh; Mehrdad Pourjafar
Source:
Veterinary immunology and immunopathology 2022 v.243 pp. 110361
ISSN:
0165-2427
Subject:
Escherichia coli; amyloid; anti-inflammatory agents; aspartate transaminase; bilirubin; biomarkers; blood serum; creatine kinase; creatinine; death; dose response; endotoxemia; glutathione peroxidase; heparin; homocysteine; immunopathology; intravenous injection; lipopolysaccharides; models; oxidative stress; serotypes; superoxide dismutase; tumor necrosis factor-alpha
Abstract:
... Endotoxemia is one of the most common inflammatory situations leading to death of ruminants. Owing to the importance of this condition, several therapeutic regimens have been proposed, evaluated and implemented to treat endotoxemia. It has recently been suggested that low molecular weight heparin may be effective in treating endotoxemia. Thus, the present experimental study was conducted to evalua ...
DOI:
10.1016/j.vetimm.2021.110361

97. Measurement of ITIH4 and Hp levels in bitches with pyometra using newly developed ELISA methods

Author:
Lourdes Soler; Marek Szczubiał; Roman Dąbrowski; Anna Płusa; Mariola Bochniarz; Piotr Brodzki; Fermín Lampreave; Matilde Piñeiro
Source:
Veterinary immunology and immunopathology 2021 v.235 pp. 110221
ISSN:
0165-2427
Subject:
biomarkers; blood serum; haptoglobins; immunopathology; medicine; pyometra
Abstract:
... Measurement of acute phase proteins (APPs) as biomarkers in canine medicine is in increasing demand. In the present study, the development and validation of two ELISA methods for the quantification of canine inter-alpha-trypsin inhibitor heavy chain 4 (ITIH4) and haptoglobin (Hp) are shown. The adequate imprecision and accuracy and wide analytical range make the developed methods appropriate to qu ...
DOI:
10.1016/j.vetimm.2021.110221

98. Molecular cloning and functional studies on magang goose toll-like receptor 5

Author:
Lian-Yun Wu; Shao-Feng Liu; Guo-Hong Hua; Ru-Min Jia; Yan-Hong Yong; Ravi Gooneratne; Xiang-Hong Ju
Source:
Veterinary immunology and immunopathology 2021 v.236 pp. 110236
ISSN:
0165-2427
Subject:
Salmonella Pullorum; Toll-like receptor 5; cecum; chickens; economic sustainability; flagellin; geese; genes; immunity; immunopathology; interleukin-1; interleukin-8; kidneys; reverse transcriptase polymerase chain reaction; sequence homology; spleen
Abstract:
... Disease outbreaks heavily impact the economic viability of animal industries. Little is known about the mechanisms of immune system-related diseases in geese. Toll-like receptors (TLRs) play a major role in the anti-inflammatory immunity process in most animal species, but they have not been studied in the Magang goose. To elucidate the role of TLRs, reverse transcription polymerase chain reaction ...
DOI:
10.1016/j.vetimm.2021.110236

99. Nasal secretory protein changes following intravenous choline administration in calves with experimentally induced endotoxaemia

Author:
M. Kocaturk; O. Eralp Inan; A. Tvarijonaviciute; B. Sahin; A.T. Baykal; M. Cansev; J.J. Ceron; Z. Yilmaz; I.H. Ulus
Source:
Veterinary immunology and immunopathology 2021 v.233 pp. 110197
ISSN:
0165-2427
Subject:
calves; choline; endotoxemia; glutathione peroxidase; immune response; intravenous injection; lipopolysaccharides; nasal cavity; oxidative stress; pathophysiology; proteins; proteome; proteomics; sumoylation; ubiquitin-protein ligase
Abstract:
... Nasal secretory fluid proteomes (NSPs) can provide valuable information about the physiopathology and prognosis of respiratory tract diseases. This study aimed to determine changes in NSP by using proteomics in calves treated with lipopolysaccharide (LPS) or LPS + choline.Healthy calves (n = 10) were treated with LPS (2 μg/kg/iv). Five minutes after LPS injection, the calves received a second iv i ...
DOI:
10.1016/j.vetimm.2021.110197

100. Neospora caninum truncated recombinant proteins formulated with liposomes and CpG-ODNs triggered a humoral immune response in cattle after immunisation and challenge

Author:
María Belén Novoa; Macarena Sarli; Ivana Gabriela Reidel; Carolina Veaute; Beatriz Valentini; María Evangelina Primo
Source:
Veterinary immunology and immunopathology 2021 v.238 pp. 110285
ISSN:
0165-2427
Subject:
Escherichia coli; Neospora caninum; adjuvants; cattle production; humoral immunity; immunization; immunoglobulin G; immunopathology; oligodeoxyribonucleotides; recombinant vaccines; tachyzoites
Abstract:
... Abortions caused by Neospora caninum are a serious problem in cattle production and require effective immunoprophylaxis. The objective of this work was to assess the humoral immune response to four recombinant (r) N. caninum antigens in cattle after immunisation and challenge. MIC1 and MIC3 proteins from the micronemes, SRS2 from the surface of tachyzoites, and GRA7 from the dense granules were ex ...
DOI:
10.1016/j.vetimm.2021.110285