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You searched for: Journal name Veterinary microbiology Remove constraint Journal name: Veterinary microbiology Publication year rev 7990-2010 Remove constraint Publication year rev: 7990-2010 Source 2010 v.142 no.3-4 Remove constraint Source: 2010 v.142 no.3-4
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1. Antimicrobial resistance and its genetic determinants in aeromonads isolated in ornamental (koi) carp (Cyprinus carpio koi) and common carp (Cyprinus carpio)

Author:
Čížek, Alois; Dolejská, Monika; Sochorová, Radana; Strachotová, Kateřina; Piačková, Veronika; Veselý, Tomáš
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 435-439
ISSN:
0378-1135
Subject:
fish farms; Cyprinus carpio; ornamental fish; Aeromonas; animal pathogenic bacteria; antibiotic resistance; genetic variation; microbial genetics; polymerase chain reaction; genes; genotype; antibiotics; prevalence
Abstract:
... The aim of this study was to evaluate antimicrobial susceptibility of Aeromonas spp. isolates from common carp and koi carp coming from randomly chosen farms. The isolates were tested for susceptibility to 8 antimicrobial agents using the standard agar dilution susceptibility test. In all isolates, PCR was used to detect the presence of tet(A-E) genes, integrase genes, and gene cassettes. From the ...
DOI:
10.1016/j.vetmic.2009.10.001
PubMed:
19900770

2. Characterization of a highly immunogenic Mycoplasma hyopneumoniae lipoprotein Mhp366 identified by peptide-spot array

Author:
Meens, J.; Bolotin, V.; Frank, R.; Böhmer, J.; Gerlach, G.-F.
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 293-302
ISSN:
0378-1135
Subject:
diagnostic techniques; genome; bacterial antigens; bacterial proteins; epitopes; bacterial pneumonia; swine diseases; lipoproteins; enzyme-linked immunosorbent assay; disease detection; animal pathogenic bacteria; disease outbreaks; Mycoplasma hyopneumoniae; immune response; microbial genetics; disease diagnosis; swine; immunoblotting; disease prevalence; vaccines; geographical variation; mycoplasmosis
Abstract:
... Enzootic pneumonia (EP) in pigs caused by Mycoplasma hyopneumoniae is a highly prevalent, chronic respiratory disease, which causes considerable economic losses in the swine industry. Most herds are vaccinated, but classical bacterin vaccines do not prevent colonization and it is not possible to detect flourishing M. hyopneumoniae infections in vaccinated herds since commonly used commercial ELISA ...
DOI:
10.1016/j.vetmic.2009.10.007
PubMed:
19913364

3. Correlation between clinico-pathological outcome and typing of Haemophilus parasuis field strains

Author:
Aragon, Virginia; Cerdà-Cuéllar, Marta; Fraile, Lorenzo; Mombarg, Mark; Nofrarías, Miquel; Olvera, Alexandre; Sibila, Marina; Solanes, David; Segalés, Joaquim
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 387-393
ISSN:
0378-1135
Subject:
piglets; disease diagnosis; disease course; microbial genetics; loci; swine diseases; serotypes; biomarkers; strain differences; animal pathogenic bacteria; disease severity; pathogen identification; Haemophilus parasuis; wild boars; nucleotide sequences; genetic variation; virulence; bacterial infections; signs and symptoms (animals and humans)
Abstract:
... Haemophilus parasuis is the etiologic agent of Glässer's disease in pigs, which is pathologically characterized by serofibrinous polyserositis and arthritis. H. parasuis include virulent and non-virulent strains and confirmation of virulence in H. parasuis is still dependent on experimental reproduction of the disease. Since the variability in virulence is supported by serotyping and genotyping (p ...
DOI:
10.1016/j.vetmic.2009.10.025
PubMed:
19945233

4. Detection of Bartonella spp. in neotropical felids and evaluation of risk factors and hematological abnormalities associated with infection

Author:
Guimaraes, A.M.S.; Brandão, P.E.; Moraes, W.; Kiihl, S.; Santos, L.C.; Filoni, C.; Cubas, Z.S.; Robes, R.R.; Marques, L.M.; Neto, R.L.; Yamaguti, M.; Oliveira, R.C.; Catão-Dias, J.L.; Richtzenhain, L.J.; Messick, J.B.; Biondo, A.W.; Timenetsky, J.
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 346-351
ISSN:
0378-1135
Subject:
ribosomal RNA; Panthera; new host records; insect vectors; zoo animals; interspecific variation; ectoparasites; Leopardus; tropics; males; females; bacterial infections; sequence analysis; host range; disease detection; hosts; polymerase chain reaction; animal pathogenic bacteria; risk factors; Siphonaptera; microbial genetics; blood cell counts; gender differences; captive animals; Puma; nucleotide sequences; Bartonella henselae; signs and symptoms (animals and humans)
Abstract:
... Although antibodies to Bartonella henselae have been described in all neotropical felid species, DNA has been detected in only one species, Leopardus wiedii. The aim of this study was to determine whether DNA of Bartonella spp. could be detected in blood of other captive neotropical felids and evaluate risk factors and hematological findings associated with infection. Blood samples were collected ...
DOI:
10.1016/j.vetmic.2009.10.002
PubMed:
19913372

5. Development and use of an indirect enzyme-linked immunosorbent assay for detection of iridovirus exposure in gopher tortoises (Gopherus polyphemus) and eastern box turtles (Terrapene carolina carolina)

Author:
Johnson, April J.; Wendland, Lori; Norton, Terry M.; Belzer, Bill; Jacobson, Elliott R.
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 160-167
ISSN:
0378-1135
Subject:
Gopherus polyphemus; Emydidae; wild animals; turtles; Iridovirus; vertebrate viruses; seroprevalence; disease surveillance; enzyme-linked immunosorbent assay; blood chemistry; disease outbreaks; animal diseases; viral diseases of animals and humans; mortality; immune response; United States
Abstract:
... Iridoviruses, pathogens typically associated with fish and amphibians, have recently been shown to cause acute respiratory disease in chelonians including box turtles, red-eared sliders, gopher tortoises, and Burmese star tortoises. Case reports of natural infections in several chelonian species in the United States have been reported, however the prevalence remains unknown in susceptible populati ...
DOI:
10.1016/j.vetmic.2009.09.059
PubMed:
19931321

6. Development of a serogroup-specific DNA microarray for identification of Escherichia coli strains associated with bovine septicemia and diarrhea

Author:
Liu, Bin; Wu, Fan; Li, Dan; Beutin, Lothar; Chen, Min; Cao, Boyang; Wang, Lei
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 373-378
ISSN:
0378-1135
Subject:
cattle diseases; microarray technology; Escherichia coli; animal pathogenic bacteria; septicemia; diarrhea; Escherichia infections; serotypes; calves; disease detection; pathogen identification; bacterial antigens; strain differences; accuracy; epidemiology
Abstract:
... Escherichia coli strains belonging to serogroups O8, O9, O15, O26, O35, O78, O86, O101, O115 and O119 are commonly associated with septicemia or diarrhea in calves and pose a significant threat to the cattle industry worldwide. In this study, a microarray detection system targeting O-antigen-specific genes was developed for the identification of those serogroups. By testing against 186 E. coli and ...
DOI:
10.1016/j.vetmic.2009.10.019
PubMed:
19932572

7. Diagnostic performance of ELISA and PCR in identifying SRLV-infected sheep and goats using serum, plasma and milk samples and in early detection of infection in dairy flocks through bulk milk testing

Author:
Brinkhof, J.M.A.; Houwers, D.J.; Moll, L.; Dercksen, D.; van Maanen, C.
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 193-198
ISSN:
0378-1135
Subject:
diagnostic techniques; goats; Lentivirus; ewe milk; animal diseases; interspecific variation; viral diseases of animals and humans; antibodies; DNA; bulk milk; enzyme-linked immunosorbent assay; blood plasma; vertebrate viruses; polymerase chain reaction; immune response; microbial genetics; accuracy; disease surveillance; disease diagnosis; sheep; disease prevalence; proviruses; goat milk
Abstract:
... The objective of the study was to evaluate the diagnostic performances of the ELITEST-MVV ELISA for detection of antibodies against small ruminant lentiviruses and of two recently published PCRs for the detection of proviral DNA of SRLV in blood and corresponding individual milk samples. In addition, the feasibility of bulk milk testing was investigated by titrating ELISA positive pooled milk samp ...
DOI:
10.1016/j.vetmic.2009.09.060
PubMed:
19913363

8. Differences in replication kinetics and cell tropism between neurovirulent and non-neurovirulent EHV1 strains during the acute phase of infection in horses

Author:
Gryspeerdt, Annick C.; Vandekerckhove, A.P.; Garré, B.; Barbé, F.; Van de Walle, G.R.; Nauwynck, H.J.
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 242-253
ISSN:
0378-1135
Subject:
inhalation exposure; viremia; nasal mucosa; virus replication; basement membrane; viral diseases of animals and humans; pathogenesis; nervous system diseases; lymph nodes; virulence; B-lymphocytes; vertebrate viruses; strain differences; immune response; horse diseases; Equid alphaherpesvirus 1; nasopharynx; horses; signs and symptoms (animals and humans)
Abstract:
... Equine herpesvirus 1 (EHV1) replicates in the respiratory tract of horses, after which infected leukocytes transport virus throughout the body, resulting in abortion or nervous system disorders. Two EHV1 strains circulate in the field: neurovirulent and non-neurovirulent. To investigate differences in replication in the upper respiratory tract (URT), an experimental inoculation study in ponies was ...
DOI:
10.1016/j.vetmic.2009.10.015
PubMed:
19926232

9. Distribution and possible transmission of ampicillin- and nalidixic acid-resistant Escherichia coli within the broiler industry

Author:
Bortolaia, Valeria; Bisgaard, Magne; Bojesen, Anders Miki
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 379-386
ISSN:
0378-1135
Subject:
broiler chickens; ciprofloxacin; antibiotic resistance; nalidixic acid; genetic variation; poultry diseases; animal pathogenic bacteria; strain differences; feces; disease transmission; ampicillin; prevalence; cephalosporins; Escherichia coli
Abstract:
... This study was performed to determine the origin and transmission of β-lactam- and (fluoro)quinolone-resistant Escherichia coli in healthy, untreated broiler flocks. We focused on the dynamics of bacteria resistant to critically important antimicrobials for public and veterinary health in view of the possible link between antimicrobial resistant bacteria in farm animals and humans. By processing f ...
DOI:
10.1016/j.vetmic.2009.10.024
PubMed:
19945232

10. Emergence of a new genetic lineage of Newcastle disease virus in West and Central Africa—Implications for diagnosis and control

Author:
Cattoli, G.; Fusaro, A.; Monne, I.; Molia, S.; Le Menach, A.; Maregeya, B.; Nchare, A.; Bangana, I.; Maina, A. Garba; N'Goran Koffi, J.-N.; Thiam, H.; Bezeid, O.E.M.A.; Salviato, A.; Nisi, R.; Terregino, C.; Capua, I.
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 168-176
ISSN:
0378-1135
Subject:
chickens; geographical distribution; Newcastle disease; vertebrate viruses; epidemiology; vaccines; strain differences; amino acid sequences; vaccination; Avian orthoavulavirus 1; genotype; nucleotide sequences; immune response; viral antigens; microbial genetics; disease surveillance; disease diagnosis; Africa
Abstract:
... Newcastle disease (ND) is an OIE listed disease caused by virulent avian paramyxovirus type 1 (APMV-1) strains, which affect many species of birds and may cause severe economic losses in the poultry sector. The disease has been officially and unofficially reported in many African countries and still remains the main poultry disease in commercial and rural chickens of Africa. Unfortunately, virolog ...
DOI:
10.1016/j.vetmic.2009.09.063
PubMed:
19939590

11. Evaluation of the inclusion of a mixture of organic acids or lactulose into the feed of pigs experimentally challenged with Salmonella Typhimurium

Author:
Martín-Peláez, Sandra; Costabile, Adele; Hoyles, Lesley; Rastall, Robert A.; Gibson, Glenn R.; La Ragione, Roberto M.; Woodward, Martin J.; Mateu, Enric; Martín-Orúe, Susana M.
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 337-345
ISSN:
0378-1135
Subject:
swine feeding; disease prevention; Salmonella Typhimurium; formic acid; short chain fatty acids; dietary exposure; necropsy; nucleic acid hybridization; lactic acid; intestinal microorganisms; fermentation; cecum; ileum; feed additives; animal pathogenic bacteria; microbial colonization; species diversity; swine; lactulose; digesta; salmonellosis
Abstract:
... A mixture of organic acids and lactulose for preventing or reducing colonization of the gut by Salmonella Typhimurium was evaluated in pigs. A total of 63 4-week-old commercial piglets were randomly distributed into three different experimental dietary groups: a plain diet without additives (PD) and the same diet supplemented with either 0.4% (w/v) formic acid and 0.4% lactic acid (w/v) (AC) or 1% ...
DOI:
10.1016/j.vetmic.2009.09.061
PubMed:
19914785

12. Expression and self-assembly of virus-like particles from two genotypes of marine vesiviruses and development of an ELISA for the detection of antibodies

Author:
McClenahan, Shasta D.; Bok, Karin; Sosnovtsev, Stanislav V.; Neill, John D.; Burek, Kathy A.; Beckmen, Kimberlee B.; Smith, Alvin W.; Green, Kim Y.; Romero, Carlos H.
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 184-192
ISSN:
0378-1135
Subject:
coat proteins; geographical distribution; vertebrate viruses; virion; epidemiology; marine mammals; capsid; hosts; molecular weight; antibodies; enzyme-linked immunosorbent assay; Otariidae; antiserum; Vesivirus; microbial genetics; adsorption; Alaska
Abstract:
... Sequences encoding the major and minor capsid proteins (VP1 and VP2) from two marine vesivirus isolates (Steller sea lion viruses V810 and V1415) were engineered for expression of virus-like particles (VLPs) in the baculovirus system. The resulting VLPs were morphologically similar to native vesivirus virions. Purified VLPs were probed in immunoblots with pooled antisera specific for nine San Migu ...
Handle:
10113/42847
DOI:
10.1016/j.vetmic.2009.09.057
PubMed:
19913368
PubMed Central:
PMC2859098

13. Heterogeneity among methicillin-resistant Staphylococcus aureus from Italian pig finishing holdings

Author:
Battisti, A.; Franco, A.; Merialdi, G.; Hasman, H.; Iurescia, M.; Lorenzetti, R.; Feltrin, F.; Zini, M.; Aarestrup, F.M.
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 361-366
ISSN:
0378-1135
Subject:
swine; swine diseases; finishing; Staphylococcus aureus; animal pathogenic bacteria; methicillin; antibiotic resistance; disease surveillance; disease prevalence; strain differences; loci; genetic variation; microbial genetics; geographical distribution; disease reservoirs; Italy
Abstract:
... A survey for methicillin-resistant Staphylococcus aureus (MRSA) in finishing pig holdings was carried out in Italy in 2008. MRSA isolates were characterised by spa-, MLST-, SCCmec- and antimicrobial susceptibility typing. A prevalence of 38% (45/118, 95% CI 29.4-46.9%) positive holdings was observed. Eleven different spa-types were found among 102 MRSA isolates, clustering in lineages associated w ...
DOI:
10.1016/j.vetmic.2009.10.008
PubMed:
19914010

14. Identification of Brucella abortus genes in elk (Cervus elaphus) using in vivo-induced antigen technology (IVIAT) reveals novel markers of infection

Author:
Lowry, J.E.; Goodridge, L.; Vernati, G.; Fluegel, A.M.; Edwards, W.H.; Andrews, G.P.
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 367-372
ISSN:
0378-1135
Subject:
bacterial antigens; Cervus elaphus; cell membranes; outer membrane proteins; malate dehydrogenase; transport proteins; biomarkers; DNA; gene expression; wild animals; Brucella melitensis biovar Abortus; Escherichia coli; hosts; cattle; animal pathogenic bacteria; wildlife diseases; immune response; protein secretion; microbial genetics; Escherichia infections; disease reservoirs; brucellosis; immunoblotting; Wyoming
Abstract:
... Elk in the Greater Yellowstone Area are a major reservoir for brucellosis, which represents an obstacle to eradication of the disease in domestic livestock. Furthermore, immune responses to Brucella abortus infection in the wild host are not well-understood. In this regard, in vivo-induced antigen technology (IVIAT) was employed to identify novel B. abortus antigens expressed during infection in e ...
DOI:
10.1016/j.vetmic.2009.10.010
PubMed:
19914012

15. Molecular analysis of the bacterial microbiota in duodenal biopsies from dogs with idiopathic inflammatory bowel disease

Author:
Suchodolski, Jan S.; Xenoulis, Panagiotis G.; Paddock, Casey G.; Steiner, Jörg M.; Jergens, Albert E.
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 394-400
ISSN:
0378-1135
Subject:
dogs; dog diseases; duodenum; intestinal microorganisms; inflammatory bowel disease; intestinal mucosa; cell adhesion; species diversity; microbial genetics; ribosomal RNA; DNA; sequence analysis; nucleotide sequences; phylogeny; bacteria; microbial ecology; pathophysiology
Abstract:
... An association between mucosa-adherent commensal bacteria and inflammatory bowel disease (IBD) has been proposed for humans. There are no reports characterizing the mucosa-adherent duodenal microbiota in dogs with idiopathic IBD using molecular methods. The aim of this study was to investigate differences in the mucosa-adherent duodenal microbiota between dogs with idiopathic IBD and healthy dogs. ...
DOI:
10.1016/j.vetmic.2009.11.002
PubMed:
19959301

16. Molecular characterization of enrofloxacin resistant Actinobacillus pleuropneumoniae isolates

Author:
Wang, Yu-Chih; Chan, Jacky Peng-Wen; Yeh, Kuang-Sheng; Chang, Chao-Chin; Hsuan, Shih-Ling; Hsieh, Yi-Mei; Chang, Yi-Chih; Lai, Tan-Chen; Lin, Wen-Hsin; Chen, Ter-Hsin
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 309-312
ISSN:
0378-1135
Subject:
enrofloxacin; antibiotic resistance; Actinobacillus pleuropneumoniae; hosts; DNA topoisomerase (ATP-hydrolysing); minimum inhibitory concentration; strain differences; animal pathogenic bacteria; amino acid sequences; codons; mutants; proton pump; microbial genetics; swine; Taiwan
Abstract:
... Enrofloxacin (ER) resistant Actinobacillus pleuropneumoniae strains emerged in Taiwan in 2002. The mechanism of ER resistance in A. pleuropneumoniae has not yet been reported. A total of 48 A. pleuropneumoniae isolates were obtained from the lungs of pigs with pleuropneumonia in Taiwan between September 2007 and April 2008. Twenty-nine isolates were found to be resistant to enrofloxacin. To unders ...
DOI:
10.1016/j.vetmic.2009.09.067
PubMed:
19913366

17. Molecular detection of novel adenoviruses in fecal specimens of captive monkeys with diarrhea in China

Author:
Bányai, K.; Esona, M.D.; Liu, A.; Wang, Y.; Tu, X.; Jiang, B.
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 416-419
ISSN:
0378-1135
Subject:
gastroenteritis; vertebrate viruses; disease detection; diarrhea; microbial genetics; viral diseases of animals and humans; disease prevalence; polymerase chain reaction; laboratory animals; monkeys; phylogeny; feces; captive animals; molecular systematics; nucleotide sequences; disease incidence; Mastadenovirus; animal diseases; China
Abstract:
... Adenovirus (AdV) has been recently detected among monkeys with diarrhea in a major research primate colony in China. To better assess disease burden and epidemiology of adenoviruses in the colony, we examined the prevalence of this virus in fecal specimens by PCR using broadly reactive hexon gene-specific primers. Of the 29 strains that were characterized by sequence and phylogenetic analysis, we ...
DOI:
10.1016/j.vetmic.2009.10.014
PubMed:
19926233

18. Monitoring of Lawsonia intracellularis in breeding herd gilts

Author:
Jacobson, M.; Aspan, A.; Nordengrahn, A.; Lindberg, M.; Wallgren, P.
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 317-322
ISSN:
0378-1135
Subject:
carrier state; gilts; biosecurity; animal age; farrowing; seroprevalence; swine diseases; Lawsonia intracellularis; feces; enzyme-linked immunosorbent assay; bacterial infections; disease detection; polymerase chain reaction; animal pathogenic bacteria; animal transport; quarantine; disease diagnosis; disease prevalence; disease transmission; digestive system diseases
Abstract:
... In modern pig production, proliferative enteropathy is a common cause of diarrhoea and poor growth in young animals. This study aimed to determine the possible spread of Lawsonia intracellularis through the sale of replacement gilts and the possibility to protect the herds by adequate biosecurity measures. This was achieved by repeated sampling of 50 gilts in an infected multiplying herd, from the ...
DOI:
10.1016/j.vetmic.2009.09.034
PubMed:
19836171

19. Pheno-genotypic characterisation of Escherichia coli O157:H7 isolates from domestic and wild ruminants

Author:
Sánchez, S.; Martínez, R.; Rey, J.; García, A.; Blanco, J.; Blanco, M.; Blanco, J.E.; Mora, A.; Herrera-León, S.; Echeita, A.; Alonso, J.M.
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 445-449
ISSN:
0378-1135
Subject:
Shiga-like toxins; pulsed-field gel electrophoresis; phenotype; feces; genotype; wild animals; cattle; polymerase chain reaction; strain differences; animal pathogenic bacteria; pathogen identification; microbial genetics; Escherichia infections; disease reservoirs; toxigenic strains; sheep; disease transmission; Escherichia coli O157; goat milk; deer; Spain
Abstract:
... Shiga toxin-producing Escherichia coli (STEC) O157:H7 represents a major public health concern worldwide, with ruminants recognised as their main natural reservoir. The aim of this work was to determine the phenotypic features and genetic relationships of 46 E. coli O157:H7 isolates obtained from sheep, cattle and deer faeces and from unpasteurised goat milk in Spain over a period of 11 years. Cha ...
DOI:
10.1016/j.vetmic.2009.10.009
PubMed:
19914011

20. Phylogenetic identification of Aeromonas simiae from a pig, first isolate since species description

Author:
Fontes, Maria Conceição; Saavedra, Maria José; Monera, Arturo; Martins, Conceição; Martínez-Murcia, Antonio
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 313-316
ISSN:
0378-1135
Subject:
Aeromonas; hosts; swine; prevalence; pathogen identification; feces; host range; incidence; ribosomal DNA; microbial genetics; molecular systematics; phylogeny; strain differences
Abstract:
... The species Aeromonas simiae was first described on the basis of two strains (CIP 107798T and CIP 107797) isolated from faeces of healthy monkeys (M. fascicularis) from Mauritius, which were kept in quarantine in the Centre for Primatology, Louis Pasteur University, Strasbourg, France. In the present study, during a survey to determine the prevalence of Aeromonas at three different pig slaughterho ...
DOI:
10.1016/j.vetmic.2009.09.033
PubMed:
19853388