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1. A description of two outbreaks of capripoxvirus disease in Mongolia

Author:
Beard, P.M.; Sugar, S.; Bazarragchaa, E.; Gerelmaa, U.; Tserendorj, Sh.; Tuppurainen, E.; Sodnomdarjaa, R.
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 427-431
ISSN:
0378-1135
Subject:
sheep; goats; vertebrate viruses; Sheeppox virus; Goatpox virus; viral diseases of animals and humans; disease outbreaks; geographical distribution; host range; disease diagnosis; pathogen identification; polymerase chain reaction; neutralization tests; nucleotide sequences; sequence analysis; geographical variation; microbial genetics; Mongolia
Abstract:
... Mongolia had no reported cases of capripoxvirus disease from 1977 until an outbreak of sheeppox in 2006-2007 and then goatpox in 2008. The two outbreaks occurred in geographically distant areas of Mongolia and, most strikingly, were highly species-specific. The 2006-2007 sheeppox outbreak affected no goats and the 2008 goatpox outbreak affected no sheep despite communal herding. The diseases were ...
DOI:
10.1016/j.vetmic.2009.10.018
PubMed:
19939588
PubMed Central:
PMC2866252

2. Antimicrobial activity of propolis extract against Staphylococcus coagulase positive and Malassezia pachydermatis of canine otitis

Author:
Cardoso, Rosemari Laura; Maboni, Franciele; Machado, Gustavo; Alves, Sydney Hartz; de Vargas, Agueda Castagna
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 432-434
ISSN:
0378-1135
Subject:
dogs; dog diseases; Staphylococcus aureus; Staphylococcus intermedius; animal pathogenic bacteria; ear diseases; traditional medicine; propolis; extracts; antibacterial properties; minimum inhibitory concentration; Malassezia pachydermatis; antifungal properties; animal pathogenic fungi; mycoses; bioassays
Abstract:
... The aims of this study were to evaluate the antimicrobial potential of propolis extract by determining the minimum bactericidal concentration (MBC) for coagulase-positive Staphylococcus isolates (Staphylococcus aureus and Staphylococcus intermedius) and the minimum fungicidal concentration (MFC) for Malassezia pachydermatis isolates. The microorganisms were assayed using broth microdilution techni ...
DOI:
10.1016/j.vetmic.2009.09.070
PubMed:
19913365

3. Antimicrobial resistance and its genetic determinants in aeromonads isolated in ornamental (koi) carp (Cyprinus carpio koi) and common carp (Cyprinus carpio)

Author:
Čížek, Alois; Dolejská, Monika; Sochorová, Radana; Strachotová, Kateřina; Piačková, Veronika; Veselý, Tomáš
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 435-439
ISSN:
0378-1135
Subject:
fish farms; Cyprinus carpio; ornamental fish; Aeromonas; animal pathogenic bacteria; antibiotic resistance; genetic variation; microbial genetics; polymerase chain reaction; genes; genotype; antibiotics; prevalence
Abstract:
... The aim of this study was to evaluate antimicrobial susceptibility of Aeromonas spp. isolates from common carp and koi carp coming from randomly chosen farms. The isolates were tested for susceptibility to 8 antimicrobial agents using the standard agar dilution susceptibility test. In all isolates, PCR was used to detect the presence of tet(A-E) genes, integrase genes, and gene cassettes. From the ...
DOI:
10.1016/j.vetmic.2009.10.001
PubMed:
19900770

4. A study on the severity and relevance of porcine circovirus type 2 infections in Dutch fattening pigs with respiratory diseases

Author:
Wellenberg, G.J.; Bouwkamp, F.T.; Wolf, P.J.v.d.; Swart, W.A.J.M.; Mombarg, M.J.; de Gee, A.L.W.
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 217-224
ISSN:
0378-1135
Subject:
immunoglobulin M; necropsy; animal age; Pasteurella multocida; immunoglobulin G; viral diseases of animals and humans; antibodies; pneumonia; disease severity; finishing; mixed infection; bacterial infections; vertebrate viruses; animal pathogenic bacteria; Mycoplasma hyopneumoniae; Porcine circovirus; histopathology; postweaning multisystemic wasting syndrome; swine; food animals; disease prevalence; signs and symptoms (animals and humans); disease incidence; Netherlands
Abstract:
... This study was set up to get more insights in the severity and relevance of porcine circovirus type 2 (PCV2) infections in Dutch fattening farms in an endemic PCV2-situation with no clinical signs of post-weaning multisystemic wasting syndrome (PMWS). In part A of the study, in total 29 commercial fattening farms with varying percentages of pneumonia and pleurisy at slaughter were examined. Blood ...
DOI:
10.1016/j.vetmic.2009.10.003
PubMed:
19913367

5. Characterization of a highly immunogenic Mycoplasma hyopneumoniae lipoprotein Mhp366 identified by peptide-spot array

Author:
Meens, J.; Bolotin, V.; Frank, R.; Böhmer, J.; Gerlach, G.-F.
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 293-302
ISSN:
0378-1135
Subject:
diagnostic techniques; genome; bacterial antigens; bacterial proteins; epitopes; bacterial pneumonia; swine diseases; lipoproteins; enzyme-linked immunosorbent assay; disease detection; animal pathogenic bacteria; disease outbreaks; Mycoplasma hyopneumoniae; immune response; microbial genetics; disease diagnosis; swine; immunoblotting; disease prevalence; vaccines; geographical variation; mycoplasmosis
Abstract:
... Enzootic pneumonia (EP) in pigs caused by Mycoplasma hyopneumoniae is a highly prevalent, chronic respiratory disease, which causes considerable economic losses in the swine industry. Most herds are vaccinated, but classical bacterin vaccines do not prevent colonization and it is not possible to detect flourishing M. hyopneumoniae infections in vaccinated herds since commonly used commercial ELISA ...
DOI:
10.1016/j.vetmic.2009.10.007
PubMed:
19913364

6. Comparative performance of SNP typing and ‘Bruce-ladder' in the discrimination of Brucella suis and Brucella canis

Author:
Koylass, Mark S.; King, Amanda C.; Edwards-Smallbone, James; Gopaul, Krishna K.; Perrett, Lorraine L.; Whatmore, Adrian M.
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 450-454
ISSN:
0378-1135
Subject:
Brucella melitensis biovar Suis; diagnostic techniques; zoonoses; Brucella melitensis biovar Canis; accuracy; strain differences; animal pathogenic bacteria; single nucleotide polymorphism; pathogen identification; genotype; microbial genetics; disease diagnosis
Abstract:
... Two novel molecular assays, ‘Bruce-ladder' and SNP typing, have recently been described designed to differentiate isolates of the genus Brucella, causative organisms of the significant zoonotic disease brucellosis, at the species level. Differentiation of Brucella canis from Brucella suis by molecular approaches can be difficult and here we compare the performance of ‘Bruce-ladder' and SNP typing ...
DOI:
10.1016/j.vetmic.2009.10.013
PubMed:
19931322

7. Comparison of the effectiveness of passive (dam) versus active (piglet) immunization against porcine circovirus type 2 (PCV2) and impact of passively derived PCV2 vaccine-induced immunity on vaccination

Author:
Opriessnig, Tanja; Patterson, Abby R.; Madson, Darin M.; Pal, Narinder; Ramamoorthy, Sheela; Meng, Xiang-Jin; Halbur, Patrick G.
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 177-183
ISSN:
0378-1135
Subject:
vertebrate viruses; colostrum; food animals; vaccines; vaccination; immune response; sows; maternal immunity; Porcine circovirus; piglets; postweaning multisystemic wasting syndrome; viral antigens
Abstract:
... The objectives of this study were (1) to compare the efficacy of two different PCV2 vaccination protocols (colostrum-derived immunity versus piglet vaccination) in a conventional PCV2 growing pig challenge model and (2) to evaluate the efficacy of vaccinating piglets with the same vaccine used in the dams. Two different commercially available vaccines (VAC1; VAC2) were used in the same experiment. ...
DOI:
10.1016/j.vetmic.2009.09.056
PubMed:
19913369

8. Controversial results of the genetic analysis of a canine distemper vaccine strain

Author:
Demeter, Zoltán; Palade, Elena Alina; Hornyák, Ákos; Rusvai, Miklós
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 420-426
ISSN:
0378-1135
Subject:
restriction fragment length polymorphism; product authenticity; vertebrate viruses; Canine morbillivirus; strain differences; live vaccines; virulence; brand name products; hemagglutinins; genes; nucleotide sequences; genetic variation; microbial genetics; genetic markers; Hungary
Abstract:
... Canine distemper (CD) is a highly contagious, often fatal, multisystemic viral disease of receptive carnivores. The presence of a PsiI cleavage site on a specific location of the hemagglutinin (H) gene was found to be a hallmark of vaccine strains, thus, a previously published restriction fragment length polymorphism (RFLP) test using PsiI theoretically allows the distinction between all currently ...
DOI:
10.1016/j.vetmic.2009.10.017
PubMed:
19931324

9. Correlation between clinico-pathological outcome and typing of Haemophilus parasuis field strains

Author:
Aragon, Virginia; Cerdà-Cuéllar, Marta; Fraile, Lorenzo; Mombarg, Mark; Nofrarías, Miquel; Olvera, Alexandre; Sibila, Marina; Solanes, David; Segalés, Joaquim
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 387-393
ISSN:
0378-1135
Subject:
piglets; disease diagnosis; disease course; microbial genetics; loci; swine diseases; serotypes; biomarkers; strain differences; animal pathogenic bacteria; disease severity; pathogen identification; Haemophilus parasuis; wild boars; nucleotide sequences; genetic variation; virulence; bacterial infections; signs and symptoms (animals and humans)
Abstract:
... Haemophilus parasuis is the etiologic agent of Glässer's disease in pigs, which is pathologically characterized by serofibrinous polyserositis and arthritis. H. parasuis include virulent and non-virulent strains and confirmation of virulence in H. parasuis is still dependent on experimental reproduction of the disease. Since the variability in virulence is supported by serotyping and genotyping (p ...
DOI:
10.1016/j.vetmic.2009.10.025
PubMed:
19945233

10. Decreased susceptibility to zinc chloride is associated with methicillin resistant Staphylococcus aureus CC398 in Danish swine

Author:
Aarestrup, Frank Møller; Cavaco, Lina; Hasman, Henrik
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 455-457
ISSN:
0378-1135
Subject:
sequence analysis; Staphylococcus aureus; tetracycline; loci; antibiotic resistance; zinc; food animals; minimum inhibitory concentration; cross resistance; animal pathogenic bacteria; chlorides; nucleotide sequences; genetic variation; erythromycin; microbial genetics; methicillin; swine; Denmark
Abstract:
... A total of 31 MRSA and 60 MSSA isolated from different swine farms in Denmark were examined for their susceptibility to zinc chloride, erythromycin, penicillin and tetracycline, as well as their spa-type. mecA positive isolates were examined for their SCCmec type. The isolates were assigned to a CC-type based on their spa-type and supportive multi-locus sequence typing (MLST). No difference in sus ...
DOI:
10.1016/j.vetmic.2009.10.021
PubMed:
19939591

11. Detection of Bartonella spp. in neotropical felids and evaluation of risk factors and hematological abnormalities associated with infection

Author:
Guimaraes, A.M.S.; Brandão, P.E.; Moraes, W.; Kiihl, S.; Santos, L.C.; Filoni, C.; Cubas, Z.S.; Robes, R.R.; Marques, L.M.; Neto, R.L.; Yamaguti, M.; Oliveira, R.C.; Catão-Dias, J.L.; Richtzenhain, L.J.; Messick, J.B.; Biondo, A.W.; Timenetsky, J.
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 346-351
ISSN:
0378-1135
Subject:
ribosomal RNA; Panthera; new host records; insect vectors; zoo animals; interspecific variation; ectoparasites; Leopardus; tropics; males; females; bacterial infections; sequence analysis; host range; disease detection; hosts; polymerase chain reaction; animal pathogenic bacteria; risk factors; Siphonaptera; microbial genetics; blood cell counts; gender differences; captive animals; Puma; nucleotide sequences; Bartonella henselae; signs and symptoms (animals and humans)
Abstract:
... Although antibodies to Bartonella henselae have been described in all neotropical felid species, DNA has been detected in only one species, Leopardus wiedii. The aim of this study was to determine whether DNA of Bartonella spp. could be detected in blood of other captive neotropical felids and evaluate risk factors and hematological findings associated with infection. Blood samples were collected ...
DOI:
10.1016/j.vetmic.2009.10.002
PubMed:
19913372

12. Detection of Chlamydophila caviae and Streptococcus equi subsp. zooepidemicus in horses with signs of rhinitis and conjunctivitis

Author:
Gaede, Wolfgang; Reckling, Karl-Friedrich; Schliephake, Anette; Missal, Dirk; Hotzel, Helmut; Sachse, Konrad
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 440-444
ISSN:
0378-1135
Subject:
foals; horse diseases; Chlamydophila caviae; Streptococcus equi subsp. zooepidemicus; animal pathogenic bacteria; bacterial infections; signs and symptoms (animals and humans); disease detection; rhinitis; conjunctivitis; disease diagnosis; polymerase chain reaction; microarray technology; nucleotide sequences; microbial genetics; pathogen identification; new host records; mixed infection; synergism; host range
Abstract:
... At a stud farm of Trakehner horses, all 33 foals of a birth cohort developed conjunctivitis and serous to muco-purulent rhinitis, and 7 older horses showed recurrent signs of conjunctivitis. Examination of nasal and conjunctival swabs by bacterial and cell culture, as well as real-time PCR, ArrayTube microarray analysis and DNA sequencing led to the identification of Chlamydophila (C.) caviae (fir ...
DOI:
10.1016/j.vetmic.2009.10.011
PubMed:
19913370

13. Detection of H6 influenza antibody by blocking enzyme-linked immunosorbent assay

Author:
Chen, Yi-Tung; Juang, Rong-Huay; He, Jie-Long; Chu, Wen-Yu; Wang, Ching-Ho
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 205-210
ISSN:
0378-1135
Subject:
chickens; vertebrate viruses; disease detection; hemagglutination inhibition test; Influenza A virus; monoclonal antibodies; enzyme-linked immunosorbent assay; avian influenza; seroconversion; disease surveillance; Taiwan
Abstract:
... Low pathogenic H6N1 avian influenza viruses (AIVs) have circulated in domestic chickens since 1972 in Taiwan. Detection of avian influenza (AI) antibody is a routine work for AI control in Taiwan. The currently available commercial enzyme-linked immunosorbent assays (ELISAs) are unable to differentiate antibody responses between different subtypes. To this end, a panel of monoclonal antibodies (mA ...
DOI:
10.1016/j.vetmic.2009.09.064
PubMed:
19914784

14. Development and use of an indirect enzyme-linked immunosorbent assay for detection of iridovirus exposure in gopher tortoises (Gopherus polyphemus) and eastern box turtles (Terrapene carolina carolina)

Author:
Johnson, April J.; Wendland, Lori; Norton, Terry M.; Belzer, Bill; Jacobson, Elliott R.
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 160-167
ISSN:
0378-1135
Subject:
Gopherus polyphemus; Emydidae; wild animals; turtles; Iridovirus; vertebrate viruses; seroprevalence; disease surveillance; enzyme-linked immunosorbent assay; blood chemistry; disease outbreaks; animal diseases; viral diseases of animals and humans; mortality; immune response; United States
Abstract:
... Iridoviruses, pathogens typically associated with fish and amphibians, have recently been shown to cause acute respiratory disease in chelonians including box turtles, red-eared sliders, gopher tortoises, and Burmese star tortoises. Case reports of natural infections in several chelonian species in the United States have been reported, however the prevalence remains unknown in susceptible populati ...
DOI:
10.1016/j.vetmic.2009.09.059
PubMed:
19931321

15. Development of a serogroup-specific DNA microarray for identification of Escherichia coli strains associated with bovine septicemia and diarrhea

Author:
Liu, Bin; Wu, Fan; Li, Dan; Beutin, Lothar; Chen, Min; Cao, Boyang; Wang, Lei
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 373-378
ISSN:
0378-1135
Subject:
cattle diseases; microarray technology; Escherichia coli; animal pathogenic bacteria; septicemia; diarrhea; Escherichia infections; serotypes; calves; disease detection; pathogen identification; bacterial antigens; strain differences; accuracy; epidemiology
Abstract:
... Escherichia coli strains belonging to serogroups O8, O9, O15, O26, O35, O78, O86, O101, O115 and O119 are commonly associated with septicemia or diarrhea in calves and pose a significant threat to the cattle industry worldwide. In this study, a microarray detection system targeting O-antigen-specific genes was developed for the identification of those serogroups. By testing against 186 E. coli and ...
DOI:
10.1016/j.vetmic.2009.10.019
PubMed:
19932572

16. Diagnostic performance of ELISA and PCR in identifying SRLV-infected sheep and goats using serum, plasma and milk samples and in early detection of infection in dairy flocks through bulk milk testing

Author:
Brinkhof, J.M.A.; Houwers, D.J.; Moll, L.; Dercksen, D.; van Maanen, C.
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 193-198
ISSN:
0378-1135
Subject:
diagnostic techniques; goats; Lentivirus; ewe milk; animal diseases; interspecific variation; viral diseases of animals and humans; antibodies; DNA; bulk milk; enzyme-linked immunosorbent assay; blood plasma; vertebrate viruses; polymerase chain reaction; immune response; microbial genetics; accuracy; disease surveillance; disease diagnosis; sheep; disease prevalence; proviruses; goat milk
Abstract:
... The objective of the study was to evaluate the diagnostic performances of the ELITEST-MVV ELISA for detection of antibodies against small ruminant lentiviruses and of two recently published PCRs for the detection of proviral DNA of SRLV in blood and corresponding individual milk samples. In addition, the feasibility of bulk milk testing was investigated by titrating ELISA positive pooled milk samp ...
DOI:
10.1016/j.vetmic.2009.09.060
PubMed:
19913363

17. Differences in replication kinetics and cell tropism between neurovirulent and non-neurovirulent EHV1 strains during the acute phase of infection in horses

Author:
Gryspeerdt, Annick C.; Vandekerckhove, A.P.; Garré, B.; Barbé, F.; Van de Walle, G.R.; Nauwynck, H.J.
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 242-253
ISSN:
0378-1135
Subject:
inhalation exposure; viremia; nasal mucosa; virus replication; basement membrane; viral diseases of animals and humans; pathogenesis; nervous system diseases; lymph nodes; virulence; B-lymphocytes; vertebrate viruses; strain differences; immune response; horse diseases; Equid alphaherpesvirus 1; nasopharynx; horses; signs and symptoms (animals and humans)
Abstract:
... Equine herpesvirus 1 (EHV1) replicates in the respiratory tract of horses, after which infected leukocytes transport virus throughout the body, resulting in abortion or nervous system disorders. Two EHV1 strains circulate in the field: neurovirulent and non-neurovirulent. To investigate differences in replication in the upper respiratory tract (URT), an experimental inoculation study in ponies was ...
DOI:
10.1016/j.vetmic.2009.10.015
PubMed:
19926232

18. Distribution and possible transmission of ampicillin- and nalidixic acid-resistant Escherichia coli within the broiler industry

Author:
Bortolaia, Valeria; Bisgaard, Magne; Bojesen, Anders Miki
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 379-386
ISSN:
0378-1135
Subject:
broiler chickens; ciprofloxacin; antibiotic resistance; nalidixic acid; genetic variation; poultry diseases; animal pathogenic bacteria; strain differences; feces; disease transmission; ampicillin; prevalence; cephalosporins; Escherichia coli
Abstract:
... This study was performed to determine the origin and transmission of β-lactam- and (fluoro)quinolone-resistant Escherichia coli in healthy, untreated broiler flocks. We focused on the dynamics of bacteria resistant to critically important antimicrobials for public and veterinary health in view of the possible link between antimicrobial resistant bacteria in farm animals and humans. By processing f ...
DOI:
10.1016/j.vetmic.2009.10.024
PubMed:
19945232

19. Emergence of a new genetic lineage of Newcastle disease virus in West and Central Africa—Implications for diagnosis and control

Author:
Cattoli, G.; Fusaro, A.; Monne, I.; Molia, S.; Le Menach, A.; Maregeya, B.; Nchare, A.; Bangana, I.; Maina, A. Garba; N'Goran Koffi, J.-N.; Thiam, H.; Bezeid, O.E.M.A.; Salviato, A.; Nisi, R.; Terregino, C.; Capua, I.
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 168-176
ISSN:
0378-1135
Subject:
chickens; geographical distribution; Newcastle disease; vertebrate viruses; epidemiology; vaccines; strain differences; amino acid sequences; vaccination; Avian orthoavulavirus 1; genotype; nucleotide sequences; immune response; viral antigens; microbial genetics; disease surveillance; disease diagnosis; Africa
Abstract:
... Newcastle disease (ND) is an OIE listed disease caused by virulent avian paramyxovirus type 1 (APMV-1) strains, which affect many species of birds and may cause severe economic losses in the poultry sector. The disease has been officially and unofficially reported in many African countries and still remains the main poultry disease in commercial and rural chickens of Africa. Unfortunately, virolog ...
DOI:
10.1016/j.vetmic.2009.09.063
PubMed:
19939590

20. Enterobacteriaceae populations during experimental Salmonella infection in pigs

Author:
Guenther, Sebastian; Filter, Matthias; Tedin, Karsten; Szabo, Istvan; Wieler, Lothar H.; Nöckler, Karsten; Walk, Nicole; Schierack, Peter
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 352-360
ISSN:
0378-1135
Subject:
temporal variation; intestinal microorganisms; Escherichia coli; microbial competition; Salmonella; strain differences; animal pathogenic bacteria; digesta; jejunum; species diversity; intestinal mucosa; salmonellosis; mixed infection; population dynamics; swine
Abstract:
... Salmonella infection might affect other intestinal Enterobacteriaceae populations and possible correlations between single Enterobacteriaceae populations would help to predict subclinical Salmonella infections in pigs. In one experimental setup, weaned piglets (n =40) were infected with Salmonella and sacrificed at 3h, 24h, 72h or 28 days post-infection (p.i.). Dilutions of intestinal contents and ...
DOI:
10.1016/j.vetmic.2009.10.004
PubMed:
19931990