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- Author:
- Cornelissen, E.; Dewerchin, H.L.; Hamme, E. van; Nauwynck, H.J.
- Source:
- Veterinary microbiology 2007 v.121 no.1-2 pp. 131-137
- ISSN:
- 0378-1135
- Subject:
- surface interactions; Feline coronavirus; viral antigens; phagocytes; cats; feline infectious peritonitis; antigen detection; case studies; humoral immunity
- Abstract:
- ... Feline infectious peritonitis virus (FIPV) positive cells are present in pyogranulomas and exudates from cats with FIP. These cells belong mainly to the monocyte/macrophage lineage. How these cells survive in immune cats is not known. In this study, FIPV positive cells were isolated from pyogranulomas and exudates of 12 naturally FIPV-infected cats and the presence of two immunologic targets, vira ...
- DOI:
- 10.1016/j.vetmic.2006.11.026
-
http://dx.doi.org/10.1016/j.vetmic.2006.11.026
- Author:
- Turni, C.; Blackall, P.J.
- Source:
- Veterinary microbiology 2007 v.121 no.1-2 pp. 163-169
- ISSN:
- 0378-1135
- Subject:
- polymerase chain reaction; Actinobacillus pleuropneumoniae; pathogen identification; genes; nucleotide sequences; microbial genetics; strains; serotypes; strain differences; clonal variation
- Abstract:
- ... A restriction analysis of PCR (PCR-REA) amplified apxIVA gene has been suggested as an alternative method for serotyping of Actinobacillus pleuropneumoniae by Jaglic et al. [Jaglic, Z., Svastova, P., Rychlik, I., Nedbalcova, K., Kucerova, Z., Pavlik, I., Bartos, M., 2004. Differentiation of Actinobacillus pleuropneumoniae by PCR-REA based on sequence variability of the apxIVA gene and by ribotypin ...
- DOI:
- 10.1016/j.vetmic.2006.11.016
-
http://dx.doi.org/10.1016/j.vetmic.2006.11.016
- Author:
- Martella, V.; Elia, G.; Decaro, N.; Di Trani, L.; Lorusso, E.; Campolo, M.; Desario, C.; Parisi, A.; Cavaliere, N.; Buonavoglia, C.
- Source:
- Veterinary microbiology 2007 v.121 no.1-2 pp. 56-63
- ISSN:
- 0378-1135
- Subject:
- disease outbreaks; Influenza A virus; vaccination; horses; horse diseases; strain differences; vaccines; antigenic variation; microbial genetics; amino acid sequences; phylogeny; strains; viral diseases of animals and humans; sequence analysis; emerging diseases; equine influenza; Italy; Florida
- Abstract:
- ... In December 2005, equine influenza virus infection was confirmed as the cause of clinical respiratory disease in vaccinated horses in Apulia, Italy. The infected horses had been vaccinated with a vaccine that contained strains representatives from both the European (A/eq/Suffolk/89) and American (A/eq/Newmarket/1/93) H3N8 influenza virus lineages, and the H7N7 strain A/eq/Praga/56. Genetic charact ...
- DOI:
- 10.1016/j.vetmic.2006.11.017
-
http://dx.doi.org/10.1016/j.vetmic.2006.11.017
- Author:
- Laroucau, K.; Trichereau, A.; Vorimore, F.; Mahe, A.M.
- Source:
- Veterinary microbiology 2007 v.121 no.1-2 pp. 150-157
- ISSN:
- 0378-1135
- Subject:
- genes; polymerase chain reaction; disease diagnosis; psittacosis; Chlamydophila abortus; microbial detection; Chlamydophila psittaci; birds; strains; microbial genetics; bird diseases; pathogen identification; strain differences; sequence analysis
- Abstract:
- ... In a previous study we described the use of a new set of PCR primers (CpsiA/CpsiB) specific of the conserved pmp-family genes of Chlamydophila abortus as an efficient tool for the detection of these bacteria in ruminants including also preliminary results on avian strains. In this work, the use of this set of primers was extended to representative strains of the six major avian serovars (serovars ...
- DOI:
- 10.1016/j.vetmic.2006.11.013
-
http://dx.doi.org/10.1016/j.vetmic.2006.11.013
- Author:
- Clutterbuck, A.L.; Woods, E.J.; Knottenbelt, D.C.; Clegg, P.D.; Cochrane, C.A.; Percival, S.L.
- Source:
- Veterinary microbiology 2007 v.121 no.1-2 pp. 1-17
- ISSN:
- 0378-1135
- Subject:
- biofilm; veterinary medicine; bacteria; adaptation; bacterial adhesion; antibiotic resistance; host-pathogen relationships; biological resistance; immune response; cell communication; pathogenesis; bacterial infections
- Abstract:
- ... Bacteria are renowned for their ability to tolerate and adapt to a wide range of adverse environmental conditions. The primary mechanism that facilitates these adaptations is thought to be the capacity to form and maintain biofilms. Within a biofilm, bacteria become attached to a surface where they exist in complex communities which are able to interact with each other through intracellular commun ...
- DOI:
- 10.1016/j.vetmic.2006.12.029
-
http://dx.doi.org/10.1016/j.vetmic.2006.12.029
- Author:
- Ruggieri, A.; Di Trani, L.; Gatto, I.; Franco, M.; Vignolo, E.; Bedini, B.; Elia, G.; Buonavoglia, C.
- Source:
- Veterinary microbiology 2007 v.121 no.1-2 pp. 64-72
- ISSN:
- 0378-1135
- Subject:
- Canine coronavirus; dogs; dog diseases; apoptosis; cell culture; strains; viral enteritis; cytopathogenicity; fibrosarcoma; DNA; sequence analysis; cell lines; strain differences
- Abstract:
- ... Canine coronavirus (CCoV) is widespread in dogs in several countries and causes mild enteric illness evolving to severe enteritis in young pups. In in vitro cultures canine coronaviruses generally induce extensive cell death, however nature of the events leading to cell death remains largely unknown. We analysed the induction of cytopathic effect by CCoV in a canine fibrosarcoma cell line (A-72) i ...
- DOI:
- 10.1016/j.vetmic.2006.12.016
-
http://dx.doi.org/10.1016/j.vetmic.2006.12.016
- Author:
- Fafetine, J.M.; Tijhaar, E.; Paweska, J.T.; Neves, L.C.B.G.; Hendriks, J.; Swanepoel, R.; Coetzer, J.A.W.; Egberink, H.F.; Rutten, V.P.M.G.
- Source:
- Veterinary microbiology 2007 v.121 no.1-2 pp. 29-38
- ISSN:
- 0378-1135
- Subject:
- disease detection; serodiagnosis; Rift Valley fever virus; Rift Valley fever; nucleocapsid proteins; recombinant antigens; molecular cloning; enzyme-linked immunosorbent assay; antibody detection; sheep; cattle; goats; sheep diseases; cattle diseases; immunoglobulin G; immunoglobulin M; protein synthesis
- Abstract:
- ... Serodiagnosis of Rift Valley fever (RVF) currently relies on the use of live or inactivated whole virus as antigens. The recombinant nucleocapsid (N) protein of RVF virus was tested for diagnostic applicability in an indirect enzyme-linked immunosorbent assay (I-ELISA), using sera from experimentally infected sheep (n = 128), vaccinated sheep (n = 240), and field-collected sera from sheep (n = 251 ...
- DOI:
- 10.1016/j.vetmic.2006.11.008
-
http://dx.doi.org/10.1016/j.vetmic.2006.11.008
- Author:
- Wang, L.; Raidal, S.L.; Pizzirani, A.; Wilcox, G.E.
- Source:
- Veterinary microbiology 2007 v.121 no.1-2 pp. 18-28
- ISSN:
- 0378-1135
- Subject:
- disease detection; respiratory tract diseases; horses; horse diseases; Herpesviridae; polymerase chain reaction; viral diseases of animals and humans; microbial detection; longitudinal studies; disease prevalence
- Abstract:
- ... A nested multiplex PCR was developed as a rapid (<12 h), sensitive test for the simultaneous identification of equine herpesviruses (EHV1, EHV4, EHV2 and EHV5) in clinical samples from horses. Peripheral blood and nasal swab (NS) samples from 205 weanling Thoroughbred foals on 6 different studs over 3 consecutive seasons and from 92 adult horses without clinical signs of respiratory disease were e ...
- DOI:
- 10.1016/j.vetmic.2006.11.009
-
http://dx.doi.org/10.1016/j.vetmic.2006.11.009
- Author:
- Marien, M.; Decostere, A.; Duchateau, L.; Chiers, K.; Froyman, R.; Nauwynck, H.
- Source:
- Veterinary microbiology 2007 v.121 no.1-2 pp. 94-104
- ISSN:
- 0378-1135
- Subject:
- drug evaluation; enrofloxacin; florfenicol; amoxicillin; Ornithobacterium rhinotracheale; Escherichia coli; mixed infection; bacterial infections; poultry diseases; turkeys; animal models; disease control
- Abstract:
- ... Experimental groups of 15 susceptible 3-week-old turkeys were inoculated oculonasally with avian metapneumovirus (APV) subtype A and susceptible Escherichia coli O2:K1 and Ornithobacterium rhinotracheale (ORT) bacteria, with a 3 days interval between viral and bacterial inoculation and approximately 8 h between the two bacterial inoculations. The aims of the present study were to assess the effica ...
- DOI:
- 10.1016/j.vetmic.2006.11.012
-
http://dx.doi.org/10.1016/j.vetmic.2006.11.012
- Author:
- Boshoff, C.I.; Bastos, A.D.S.; Gerber, LJ.; Vosloo, W.
- Source:
- Veterinary microbiology 2007 v.121 no.1-2 pp. 45-55
- ISSN:
- 0378-1135
- Subject:
- genetic techniques and protocols; African swine fever virus; African swine fever; disease outbreaks; longitudinal studies; pathogen identification; microbial genetics; phylogeny; sequence analysis; genotype; epidemiological studies; Southern Africa
- Abstract:
- ... African swine fever (ASF) is a highly lethal and economically significant disease of domestic pigs in the southern African sub-region, where outbreaks regularly occur. There is anecdotal evidence suggesting that trans-boundary movement of infected animals may have played a role in precipitating widespread outbreaks in the past, however, since the 1970s outbreaks have generally been more localised, ...
- DOI:
- 10.1016/j.vetmic.2006.11.007
-
http://dx.doi.org/10.1016/j.vetmic.2006.11.007
- Author:
- Ning, H.; Niu, Z.; Yu, R.; Zhang, P.; Dong, S.; Li, Z.
- Source:
- Veterinary microbiology 2007 v.121 no.1-2 pp. 125-130
- ISSN:
- 0378-1135
- Subject:
- pathogen identification; genotype; Hepatitis E virus; swine diseases; nucleotide sequences; feces; swine; farms; case studies; disease incidence; sequence analysis; disease outbreaks; China
- Abstract:
- ... Strains of hepatitis E virus (HEV) genotypes 1 and 4 have been detected on the Chinese mainland although there have been no previous reports of zoonotic genotype 3 HEV. In the present study, 65 swine fecal specimens were collected from five pig farms located in different Shanghai suburbs. RT-PCR and nested PCR were undertaken using partial nucleotide sequences of Open Reading Frame 2 (ORF2) of HEV ...
- DOI:
- 10.1016/j.vetmic.2006.11.006
-
http://dx.doi.org/10.1016/j.vetmic.2006.11.006
- Author:
- Schneeweiss, W.; Stanek, C.; Wagner, M.; Hein, I.
- Source:
- Veterinary microbiology 2007 v.121 no.1-2 pp. 189-193
- ISSN:
- 0378-1135
- Subject:
- polymerase chain reaction; inhibitors; DNA; synovial fluid; cattle; horses; swine; new methods; microbial ecology; analytical kits; disease diagnosis
- Abstract:
- ... The potential of five different commercial DNA isolation methods to remove real-time PCR inhibitors from the synovial fluid of horses, cattle and pigs was investigated. All kits with the exception of one included a silica column-based purification of the DNA. With the fifth kit, DNA purification is achieved by removing contaminating macromolecules by a desalting process. We used a recently develop ...
- DOI:
- 10.1016/j.vetmic.2006.12.004
-
http://dx.doi.org/10.1016/j.vetmic.2006.12.004
- Author:
- Silva, E.F.; Brod, C.S.; Cerqueira, G.M.; Bourscheidt, D.; Seyffert, N.; Queiroz, A.; Santos, C.S.; Ko, A.I.; Dellagostin, O.A.
- Source:
- Veterinary microbiology 2007 v.121 no.1-2 pp. 144-149
- ISSN:
- 0378-1135
- Subject:
- Leptospira noguchii; sheep; sheep diseases; sequence analysis; pathogen identification; nucleotide sequences; leptospirosis; epidemiological studies; molecular epidemiology
- Abstract:
- ... The main goal of this study was to obtain new isolates of Leptospira spp. from sheep. A total of 10 kidney samples and 44 blood samples were collected from sheep slaughtered in Pelotas, Southern Brazil. One isolate was obtained which was identified by 16S rRNA gene sequencing and serogrouping to be Leptospira noguchii serogroup Autumnalis. Microscopic agglutination test (MAT) evaluation revealed t ...
- DOI:
- 10.1016/j.vetmic.2006.11.010
- PubMed:
- 17222993
- PubMed Central:
- PMC1868676
-
http://dx.doi.org/10.1016/j.vetmic.2006.11.010
- Author:
- Stephan, B.; Greife, H.A.; Pridmore, A.; Silley, P.
- Source:
- Veterinary microbiology 2007 v.121 no.1-2 pp. 194-195
- ISSN:
- 0378-1135
- Subject:
- mutants; Porphyromonas gingivalis; antibiotic resistance; resistance management; antibiotics; minimum inhibitory concentration; disease control; clonal variation; drug therapy
- DOI:
- 10.1016/j.vetmic.2007.01.003
-
http://dx.doi.org/10.1016/j.vetmic.2007.01.003
- Author:
- El Zubeir, I.E.M.; Kanbar, T.; Alber, J.; Lammler, C.; Akineden, O.; Weiss, R.; Zschock, M.
- Source:
- Veterinary microbiology 2007 v.121 no.1-2 pp. 170-176
- ISSN:
- 0378-1135
- Subject:
- phenotype; genotype; methicillin; antibiotic resistance; Staphylococcus intermedius; Staphylococcus aureus; clinical examination; microbial detection; pathogen identification; strains; strain differences; veterinary clinics; oxacillin; Germany
- Abstract:
- ... Methicillin/oxacillin resistance of 10 S. intermedius strains was investigated by conventional and molecular methods. The strains tested had been isolated in Germany during routine veterinary microbiological examinations of specimens from a small animal clinic between May and September 2005. Epidemiological relationships of the strains were studied by macrorestriction analysis of their chromosomal ...
- DOI:
- 10.1016/j.vetmic.2006.11.014
-
http://dx.doi.org/10.1016/j.vetmic.2006.11.014
- Author:
- Litster, A.; Moss, S.M.; Honnery, M.; Rees, B.; Trott, D.J.
- Source:
- Veterinary microbiology 2007 v.121 no.1-2 pp. 182-188
- ISSN:
- 0378-1135
- Subject:
- disease prevalence; bacterial colonization; cats; cat diseases; bacterial infections; etiology; bacteria; signs and symptoms (animals and humans); Staphylococcus felis; urinary tract; urinary tract diseases; molecular epidemiology
- Abstract:
- ... This study investigated the prevalence of bacterial pathogens of the urinary tract in Australian cats. Urine was collected by cystocentesis and subjected to urinalysis, bacterial culture and susceptibility testing. A total of 126 isolates were obtained from 107 culture-positive cats. Escherichia coli was most commonly isolated (37.3% of isolates) with the majority of isolates showing susceptibilit ...
- DOI:
- 10.1016/j.vetmic.2006.11.025
-
http://dx.doi.org/10.1016/j.vetmic.2006.11.025
- Author:
- Baczynska, A.; Fedder, J.; Schougaard, H.; Christiansen, G.
- Source:
- Veterinary microbiology 2007 v.121 no.1-2 pp. 138-143
- ISSN:
- 0378-1135
- Subject:
- Mycoplasma; semen; cervical mucus; stallions; mares; mycoplasmosis; disease detection; animal fertility; reproductive disorders; genitalia; longitudinal studies; microbial detection; Denmark
- Abstract:
- ... The reproduction rate of horses is one of the lowest within domestic livestock despite advances the veterinary medicine. Infertility in horses may be due mainly to the lack of suitable selection criteria in the breeding of horses. However, acquired infertility due to genital, bacterial infections may occur. Mycoplasmas have been implicated in genital disorders and infertility of many species inclu ...
- DOI:
- 10.1016/j.vetmic.2006.11.021
-
http://dx.doi.org/10.1016/j.vetmic.2006.11.021
- Author:
- Pinto, P.M.; Chemale, G.; Castro, L.A. de; Costa, A.P.M.; Kich, J.D.; Vainstein, M.H.; Zaha, A.; Ferreira, H.B.
- Source:
- Veterinary microbiology 2007 v.121 no.1-2 pp. 83-93
- ISSN:
- 0378-1135
- Subject:
- proteome; Mycoplasma hyopneumoniae; strain differences; antigens; immunization; swine; strains; swine diseases; proteins; immunoblotting
- Abstract:
- ... Mycoplasma hyopneumoniae is an important pathogen for pigs, being the causative agent of enzootic pneumonia. Recently, the genome sequences of three strains, J, 7448 and 232 have been reported. Here, we describe the results of a proteomic analysis, based on two-dimensional gel electrophoresis of soluble protein extracts, immunoblot and mass spectrometry, which was carried out aiming the identifica ...
- DOI:
- 10.1016/j.vetmic.2006.11.018
-
http://dx.doi.org/10.1016/j.vetmic.2006.11.018
- Author:
- Akineden, O.; Alber, J.; Lammler, C.; Weiss, R.; Siebert, U.; Foster, G.; Tougaard, S.; Brasseur, S.M.J.M.; Reijnders, P.J.H.
- Source:
- Veterinary microbiology 2007 v.121 no.1-2 pp. 158-162
- ISSN:
- 0378-1135
- Subject:
- sequence homology; Streptococcus equi subsp. zooepidemicus; strains; Phoca vitulina; Halichoerus grypus; seals; longitudinal studies; bacterial infections; animal diseases; strain differences; phylogeny; epidemiological studies; Phocine distemper virus; disease outbreaks; North Sea
- Abstract:
- ... The present study was designed to identify 15 beta-hemolytic streptococci isolated during a period between 1988 and 2005 from nine harbour seals and six grey seals from various origins of the North Sea. All isolates were identified as Streptococcus equi subsp. zooepidemicus. The bacteria were additionally investigated for relatedness by restriction fragment length polymorphism analysis of PCR ampl ...
- DOI:
- 10.1016/j.vetmic.2006.11.015
-
http://dx.doi.org/10.1016/j.vetmic.2006.11.015
- Author:
- Hunt, M.E.; Brown, D.R.
- Source:
- Veterinary microbiology 2007 v.121 no.1-2 pp. 73-82
- ISSN:
- 0378-1135
- Subject:
- sialidase; Mycoplasma alligatoris; mycoplasmosis; lungs; fibroblasts; animal diseases; enzyme activity; apoptosis; virulence; respiratory tract diseases; alligators; cell culture; protein synthesis; signal transduction; in vitro studies
- Abstract:
- ... Mycoplasma alligatoris causes acute lethal cardiopulmonary disease of susceptible hosts. A survey of its genome implicated sialidase and hyaluronidase, synergistic regulators of hyaluronan receptor CD44-mediated signal transduction leading to apoptotic cell death, as virulence factors of M. alligatoris. In this study, after the existence of a CD44 homolog in alligators was established by immunolab ...
- DOI:
- 10.1016/j.vetmic.2006.10.009
- PubMed:
- 17276629
- PubMed Central:
- PMC1853318
-
http://dx.doi.org/10.1016/j.vetmic.2006.10.009