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You searched for: Journal Veterinary microbiology Remove constraint Journal: Veterinary microbiology Publication Year 2007 Remove constraint Publication Year: 2007 Subject pathogen identification Remove constraint Subject: pathogen identification
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1. Detection by RT-PCR and genetic characterization of canine distemper virus from vaccinated and non-vaccinated dogs in Argentina

Author:
Calderon, M.G.; Remorini, P.; Periolo, O.; Iglesias, M.; Mattion, N.; La Torre, J.
Source:
Veterinary microbiology 2007 v.125 no.3-4 pp. 341-349
ISSN:
0378-1135
Subject:
phylogeny; vaccination; strains; Canine morbillivirus; pathotypes; sequence analysis; disease detection; dogs; pathogen identification; pathogenicity; canine distemper; hemagglutinins; molecular epidemiology; microbial genetics; live vaccines; reverse transcriptase polymerase chain reaction; rapid methods; genetic variation; RNA; nucleocapsid proteins; Argentina
Abstract:
... RT-PCR was used to detect canine distemper virus (CDV) RNA in clotted blood from Argentine domestic dogs. The NP gene was detected in 73 out of 99 blood samples analyzed. The deduced amino acid sequence of these gene fragments showed 100% identity with the sequence of other wild-type and vaccine strains. A fragment of the hemagglutinin gene was amplified from 24 (32.9%) of the NP-RNA-positive clin ...
DOI:
10.1016/j.vetmic.2007.05.020

2. Evidence for plasmid-mediated tetracycline resistance in Paenibacillus larvae, the causal agent of American Foulbrood (AFB) disease in honeybees

Author:
Alippi, A.M.; Lopez, A.C.; Reynaldi, F.J.; Grasso, D.H.; Aguilar, O.M.
Source:
Veterinary microbiology 2007 v.125 no.3-4 pp. 290-303
ISSN:
0378-1135
Subject:
epidemiological studies; tetracycline; phenotype; antibiotic resistance; Paenibacillus larvae; minimum inhibitory concentration; geographical variation; honey bees; plasmids; American foul brood; pathogen identification; genes; molecular epidemiology; reference standards; genetic variation; oxytetracycline; virulence; bacterial infections; defense mechanisms
Abstract:
... Paenibacillus larvae is the causal agent of American Foulbrood (AFB) disease, the most virulent bacterial disease of honeybee (Apis mellifera L.) brood. Oxytetracycline is the main antibiotic used for prevention and control of AFB. Using the polymerase chain reaction, isolates were screened for the presence of the tetracycline resistance tet(K) and tet(L) determinants. Four isolates (5%), which co ...
DOI:
10.1016/j.vetmic.2007.05.018

3. From Haemobartonella to hemoplasma: Molecular methods provide new insights

Author:
Willi, B.; Boretti, F.S.; Tasker, S.; Meli, M.L.; Wengi, N.; Reusch, C.E.; Lutz, H.; Hofmann-Lehmann, R.
Source:
Veterinary microbiology 2007 v.125 no.3-4 pp. 197-209
ISSN:
0378-1135
Subject:
disease reservoirs; anemia; antibiotics; cats; disease prevalence; strain differences; risk factors; reverse transcriptase polymerase chain reaction; mycoplasmosis; disease transmission; pathogen identification; pathogenicity; cat diseases; molecular epidemiology; Mycoplasma haemofelis; pathogenesis; Mycoplasma haemominutum
Abstract:
... Hemotropic mycoplasmas (aka hemoplasmas) are the causative agents of infectious anemia in numerous mammalian species. Originally known as Haemobartonella and Eperythrozoon species, these organisms have been reclassified within the genus Mycoplasma. The development of new molecular assays has expanded our knowledge of this heterogeneous group of agents and allowed us to study their epidemiology and ...
DOI:
10.1016/j.vetmic.2007.06.027

4. Occurrence of multidrug resistant Salmonella in antimicrobial-free (ABF) swine production systems

Author:
Thakur, S.; Tadesse, D.A.; Morrow, M.; Gebreyes, W.A.
Source:
Veterinary microbiology 2007 v.125 no.3-4 pp. 362-367
ISSN:
0378-1135
Subject:
tetracycline; extensive farming; Salmonella Typhimurium; streptomycin; slaughter; swine diseases; multiple drug resistance; farms; risk assessment; epidemiological studies; slaughterhouses; serotypes; pathogen identification; swine; antibiotic resistance; disease prevalence; disease transmission; environmental factors; salmonellosis; intensive livestock farming; North Carolina
Abstract:
... This cross-sectional study was conducted to determine the prevalence and antimicrobial resistance of Salmonella species in swine reared in the intensive (indoor) and extensive (outdoor) ABF production systems at farm and slaughter in North Carolina, U.S.A. We sampled a total of 279 pigs at farm (extensive 107; intensive 172) and collected 274 carcass swabs (extensive 124; intensive 150) at slaught ...
DOI:
10.1016/j.vetmic.2007.05.025

5. Phenotypic and genotypic characterization of antimicrobial resistance in faecal enterococci from wild boars (Sus scrofa)

Author:
Poeta, P.; Costa, D.; Igrejas, G.; Rodrigues, J.; Torres, C.
Source:
Veterinary microbiology 2007 v.125 no.3-4 pp. 368-374
ISSN:
0378-1135
Subject:
enteropathogens; strains; drug resistance; phenotype; swine diseases; feces; risk assessment; genotype; bacterial infections; defense mechanisms; epidemiological studies; Enterococcus; wildlife diseases; Sus scrofa; pathogen identification; wild boars; microbial genetics; disease reservoirs; disease prevalence; disease transmission; digestive system diseases; anti-infective agents; Portugal
Abstract:
... The objective was to study the prevalence of antimicrobial resistance and the mechanisms implicated in faecal enterococci of wild boars in Portugal. One hundred and thirty-four enterococci (67 E. faecium, 54 E. hirae, 2 E. faecalis, 2 E. durans and 9 Enterococcus spp.) were recovered from 67 wild boars (two isolates/sample), and were further analysed. High percentages of resistance were detected f ...
DOI:
10.1016/j.vetmic.2007.06.003

6. Pulmonary cryptococcosis in slaughtered sheep: Anatomopathology and culture

Author:
Lemos, L.S.; Santos, A.S. de O. dos; Vieira-da-Motta, O.; Texeira, G.N.; Carvalho, E.C.Q. de
Source:
Veterinary microbiology 2007 v.125 no.3-4 pp. 350-354
ISSN:
0378-1135
Subject:
sheep; sheep diseases; respiratory tract diseases; mycoses; Cryptococcus neoformans; disease detection; case studies; epidemiological studies; pathogen identification; lungs; tissue analysis; cryopreservation; histopathology; yeasts; epithelium; viability; microscopy; differential staining; in vitro culture; risk assessment; disease transmission; zoonoses; Brazil
Abstract:
... Cryptococcosis is considered a zoonosis but is rare in stock animals. In humans it is imputed to be a mortality cause in immunocompromised patients. This case study reports the anatomopathology of respiratory cryptococcosis in ovine and the recuperation and identification of Cryptococcus neoformans var. neoformans from 1-year frozen lung tissue sample. The findings from haematoxylin/eosin staining ...
DOI:
10.1016/j.vetmic.2006.11.028

7. Sequence and phylogenetic analysis of the 16S rRNA gene of Ehrlichia canis strains in dogs with clinical monocytic ehrlichiosis

Author:
Siarkou, V.I.; Mylonakis, M.E.; Bourtzi-Hatzopoulou, E.; Koutinas, A.F.
Source:
Veterinary microbiology 2007 v.125 no.3-4 pp. 304-312
ISSN:
0378-1135
Subject:
sequence analysis; ribosomal RNA; dog diseases; dogs; polymerase chain reaction; phylogeny; genetic variation; disease severity; pathogen identification; genotype; molecular epidemiology; genes; nucleotide sequences; strains; ehrlichiosis; virulence; Ehrlichia canis; disease course; Greece
Abstract:
... The purpose of this study was to characterize, at the molecular level, the Ehrlichia canis strains involved in naturally occurring canine monocytic ehrlichiosis (CME) in Greece, and to investigate if any sequence diversity exists between the 16S rRNA genes of those involved in the mild non-myelosuppressive or the severe myelosuppressive form of CME. To this end, amplification of the ehrlichial 16S ...
DOI:
10.1016/j.vetmic.2007.05.021

8. First isolation of Haemophilus parasuis and other NAD-dependent Pasteurellaceae of swine from European wild boars

Author:
Olvera, A.; Cerdà-Cuéllar, M.; Mentaberre, G.; Casas-Diaz, E.; Lavin, S.; Marco, I.; Aragon, V.
Source:
Veterinary microbiology 2007 v.125 no.1-2 pp. 182-186
ISSN:
0378-1135
Subject:
host range; swine diseases; strain differences; wildlife diseases; pathogen identification; Haemophilus parasuis; Actinobacillus minor; genotype; nucleotide sequences; wild boars; nasal mucosa; microbial genetics; Actinobacillus indolicus; Europe
Abstract:
... Haemophilus parasuis is a colonizer of the upper respiratory tract of pigs and the etiological agent of Glässer's disease, which is characterized by a fibrinous polyserositis, meningitis and arthritis. Glässer's disease has never been reported in wild boar (Sus scrofa), although antibodies against H. parasuis have been detected. The goal of this study was to confirm the presence of this bacterium ...
DOI:
10.1016/j.vetmic.2007.05.003

9. Identification and differentiation of canine Mycoplasma isolates by 16S-23S rDNA PCR-RFLP

Author:
Spergser, J.; Rosengarten, R.
Source:
Veterinary microbiology 2007 v.125 no.1-2 pp. 170-174
ISSN:
0378-1135
Subject:
restriction fragment length polymorphism; diagnostic techniques; disease detection; ribosomal RNA; restriction mapping; dog diseases; dogs; polymerase chain reaction; strain differences; mycoplasmosis; pathogen identification; Mycoplasma; genitalia; microbial genetics; disease diagnosis
Abstract:
... Conventional serological methods for the identification of canine mycoplasma isolates depend on the availability of a panel of species-specific diagnostic antisera and are not always reliable in terms of specificity. To enable simultaneous identification of field isolates, PCR-RFLP analysis of the 16S-23S rRNA intergenic spacer region was used to characterize the type strains of the 12 currently d ...
DOI:
10.1016/j.vetmic.2007.04.045

10. Characterization of an H5N1 avian influenza virus from Taiwan

Author:
Lee, M.S.; Deng, M.C.; Lin, Y.J.; Chang, C.Y.; Shieh, H.K.; Shiau, J.Z.; Huang, C.C.
Source:
Veterinary microbiology 2007 v.124 no.3-4 pp. 193-201
ISSN:
0378-1135
Subject:
phylogeny; pathotypes; virulence; ducks; amino acid sequences; mice; pathogen identification; hemagglutinins; molecular epidemiology; microbial genetics; chickens; disease reservoirs; Influenza A virus; disease transmission; viral proteins; avian influenza; nucleotide sequences; animal models; Taiwan
Abstract:
... In 2003, an avian influenza (AI) virus of H5N1 subtype (A/Duck/China/E319-2/03; Dk/CHN/E319-2/03) was isolated from a smuggled duck in Kinmen Island of Taiwan. Phylogenetic analysis and pairwise comparison of nucleotide and amino acid sequences revealed that the virus displayed high similarity to the H5N1 viruses circulating in Asia during 2004 and 2005. The hemagglutinin (HA) protein of the virus ...
DOI:
10.1016/j.vetmic.2007.04.021

11. Differentiation of feline coronavirus type I and II infections by virus neutralization test

Author:
Shiba, N.; Maeda, K.; Kato, H.; Mochizuki, M.; Iwata, H.
Source:
Veterinary microbiology 2007 v.124 no.3-4 pp. 348-352
ISSN:
0378-1135
Subject:
disease detection; seroprevalence; serodiagnosis; feline infectious peritonitis; cats; serotypes; neutralization tests; disease prevalence; Feline coronavirus; pathotypes; pathogen identification; molecular epidemiology; antigenic variation; Japan
Abstract:
... Feline coronavirus (FCoV) is divided into two types I and II, based on their growth in vitro and antigenicity. In this study, virus neutralization (VN) test was applied for type differentiation of FCoV infections. Sera of cats which were clinically and serologically diagnosed as feline infectious peritonitis (FIP) possessed significantly higher VN titers to type I FCoV, and sera from cats experime ...
DOI:
10.1016/j.vetmic.2007.04.031

12. Genetic and antigenic characterization of bovine viral diarrhea virus type 2 isolated from Indian goats (Capra hircus)

Author:
Mishra, N.; Dubey, R.; Rajukumar, K.; Tosh, C.; Tiwari, A.; Pitale, S.S.; Pradhan, H.K.
Source:
Veterinary microbiology 2007 v.124 no.3-4 pp. 340-347
ISSN:
0378-1135
Subject:
disease detection; serotypes; goats; phylogeny; reverse transcriptase polymerase chain reaction; mutation; bovine viral diarrhea; pathogen identification; Bovine viral diarrhea virus 1; molecular epidemiology; antigenic variation; goat diseases; India
Abstract:
... Recent studies have shown that bovine viral diarrhea virus (BVDV) type 1 is widely prevalent in Indian cattle. In a surveillance of randomly collected 562 blood samples from seven states during 2004-2006, BVDV type 2 was detected in two native Indian goats by nested reverse transcription polymerase chain reaction (nRT-PCR). The virus isolated from them was classified antigenically as BVDV 2 on the ...
DOI:
10.1016/j.vetmic.2007.04.023

13. Real-time PCR for detection and differentiation of Streptococcus equi subsp. equi and Streptococcus equi subsp. zooepidemicus

Author:
Båverud, V.; Johansson, S.K.; Aspan, A.
Source:
Veterinary microbiology 2007 v.124 no.3-4 pp. 219-229
ISSN:
0378-1135
Subject:
horses; strangles; Streptococcus equi subsp. equi; disease detection; disease diagnosis; microbial genetics; molecular epidemiology; pathogen identification; strains; Streptococcus equi subsp. zooepidemicus; reverse transcriptase polymerase chain reaction; nucleotide sequences; genes; superoxide dismutase; ribosomal RNA; rapid methods
Abstract:
... Strangles is a contagious equine disease caused by Streptococcus equi subsp. equi. In this study, clinical strains of S. equi (n = 24) and Streptococcus equi subsp. zooepidemicus (n = 24) were genetically characterized by sequencing of the 16S rRNA and sodA genes in order to devise a real-time PCR system that can detect S. equi and S. zooepidemicus and distinguish between them. Sequencing demonstr ...
DOI:
10.1016/j.vetmic.2007.04.020

14. An unusual Actinobacillus equuli strain isolated from a rabbit with Tyzzer's disease

Author:
Moyaert, H.; Decostere, A.; Baele, M.; Hermans, K.; Tavernier, P.; Chiers, K.; Haesebrouck, F.
Source:
Veterinary microbiology 2007 v.124 no.1-2 pp. 184-186
ISSN:
0378-1135
Subject:
rabbits; diagnostic techniques; disease detection; liver; disease course; mixed infection; rabbit diseases; pathogen identification; Actinobacillus equuli; molecular epidemiology; hemolysis; lungs; pathotypes; case studies; Clostridium piliforme; bacterial infections; adults; disease diagnosis
Abstract:
... Actinobacillus equuli was isolated in pure culture from the liver and lungs of an adult rabbit with Tyzzer's disease (Clostridium piliforme). Based on the haemolytic features on blood agar plates, a positive reaction in the CAMP-test, hydrolysis of esculin, the inability to ferment l-arabinose, tDNA-PCR and sequencing of the 16S rRNA gene, the isolate was classified as A. equuli subsp. haemolyticu ...
DOI:
10.1016/j.vetmic.2007.04.013

15. Low pathogenicity H5N2 avian influenza outbreak in Japan during the 2005-2006

Author:
Okamatsu, M.; Saito, T.; Yamamoto, Y.; Mase, M.; Tsuduku, S.; Nakamura, K.; Tsukamoto, K.; Yamaguchi, S.
Source:
Veterinary microbiology 2007 v.124 no.1-2 pp. 35-46
ISSN:
0378-1135
Subject:
chickens; epidemiological studies; antigenic variation; virus transmission; serotypes; Influenza A virus; phylogeny; disease outbreaks; pathogen identification; airborne microorganisms; avian influenza; molecular epidemiology; pathotypes; virulence; disease surveillance; Japan
Abstract:
... At the end of May 2005, a low-pathogenicity avian influenza (LPAI) virus of subtype H5N2 was isolated for the first time from chickens in Japan. Through active and epidemiological surveillance, 5.78 million chickens on 41 farms were found to be affected and 16 H5N2 viruses were isolated. Antigenic analysis revealed antigenic similarity of these isolates. Phylogenetic analysis showed that they orig ...
DOI:
10.1016/j.vetmic.2007.04.025

16. Molecular epidemiology of bluetongue virus in Portugal during 2004-2006 outbreak

Author:
Barros, S.C.; Ramos, F.; Luis, T.M.; Vaz, A.; Duarte, M.; Henriques, M.; Cruz, B.; Fevereiro, M.
Source:
Veterinary microbiology 2007 v.124 no.1-2 pp. 25-32
ISSN:
0378-1135
Subject:
sheep; sheep diseases; emerging diseases; bluetongue; Bluetongue virus; disease outbreaks; molecular epidemiology; pathogen identification; serotypes; antigenic variation; phylogeny; nucleotide sequences; genome; protein structure; microbial genetics; vaccination; vaccines; Portugal
Abstract:
... After 44 years of epidemiological silence, bluetongue virus (BTV) was reintroduced in Portugal in the autumn of 2004. The first clinical cases of bluetongue disease (BT) were notified in sheep farms located in the South of Portugal, close to the Spanish border. A total of six BTV, five of serotype 4 and one of serotype 2 were isolated from sheep and cattle during the 2004-006 epizootics. The nucle ...
DOI:
10.1016/j.vetmic.2007.04.014

17. Molecular epidemiology of bovine noroviruses in South Korea

Author:
Park, S.I.; Jeong, C.; Kim, H.H.; Park, S.H.; Park, S.J.; Hyun, B.H.; Yang, D.K.; Kim, S.K.; Kang, M.I.; Cho, K.O.
Source:
Veterinary microbiology 2007 v.124 no.1-2 pp. 125-133
ISSN:
0378-1135
Subject:
enteropathogens; phylogeny; strains; cattle diseases; diarrhea; viral diseases of animals and humans; feces; genotype; epidemiological studies; amino acid sequences; pathogen identification; molecular epidemiology; Norovirus; calves; microbial genetics; disease prevalence; reverse transcriptase polymerase chain reaction; nucleotide sequences; genetic variation; South Korea
Abstract:
... Since the prevalence of bovine norovirus (BNoV) and their genetic diversity have only been reported in the USA, England, Germany and The Netherlands, this study examined the prevalence and genetic diversity of BNoVs in diarrheic calves in South Korea using 645 diarrheic fecal specimens from calves by RT-PCR and nested PCR assays. Overall, 9.3% of the diarrheic fecal samples tested positive for BNo ...
DOI:
10.1016/j.vetmic.2007.03.010

18. Molecular epidemiology of foot-and-mouth disease virus types A and O isolated in Argentina during the 2000-2002 epizootic

Author:
Konig, G.A.; Palma, E.L.; Maradei, E.; Piccone, M.E.
Source:
Veterinary microbiology 2007 v.124 no.1-2 pp. 1-15
ISSN:
0378-1135
Subject:
animal diseases; livestock; microbial genetics; foot-and-mouth disease; Foot-and-mouth disease virus; disease outbreaks; molecular epidemiology; pathogen identification; serotypes; antigenic variation; phylogeny; nucleotide sequences; amino acid sequences; emerging diseases; disease control; vaccination; vaccines; protective effect; Argentina
Abstract:
... During 2000-2002 a foot-and-mouth disease (FMD) epizootic affected Argentina and spread across the country resulting in more than 2500 outbreaks. In order to study the evolution of the FMD viruses (FMDV) and help with disease control measures, a genetic characterization and phylogenetic analysis was performed of 43 field isolates representative of the epizootic. The nucleotide sequence of the VP1- ...
DOI:
10.1016/j.vetmic.2007.03.015

19. Phenotypic and genotypic characterization of Paenibacillus larvae isolates

Author:
Antunez, K.; Piccini, C.; Castro-Sowinski, S.; Rosado, A.S.; Seldin, L.; Zunino, P.
Source:
Veterinary microbiology 2007 v.124 no.1-2 pp. 178-183
ISSN:
0378-1135
Subject:
American foul brood; bacterial proteins; DNA fingerprinting; strains; gel electrophoresis; phenotype; genotype; bacterial infections; epidemiological studies; geographical distribution; Apis mellifera; polymerase chain reaction; honey bees; pathogen identification; molecular epidemiology; antibiotic resistance; Paenibacillus larvae; disease prevalence; genetic variation; Argentina; Uruguay
Abstract:
... Paenibacillus larvae is the causative agent of American Foulbrood (AFB), a severe disease of honeybees (Apis melifera). The aim of this work was to develop a strategy for the subtyping and the epidemiological analysis of P. larvae. Phenotypic characterisation, susceptibility to several antibiotics, electrophoresis of whole bacterial proteins, rep-PCR, ribotyping and DGGE were assessed using a coll ...
DOI:
10.1016/j.vetmic.2007.04.012

20. Analytical applications of Fourier transform-infrared (FT-IR) spectroscopy in microbiology and prion research

Author:
Beekes, M.; Lasch, P.; Naumann, D.
Source:
Veterinary microbiology 2007 v.123 no.4 pp. 305-319
ISSN:
0378-1135
Subject:
biomedical research; microbiology; animal diseases; human diseases; prion diseases; Fourier transform infrared spectroscopy; prions; protein folding; tissue analysis; biomarkers; molecular epidemiology; pathogen identification; rapid methods
Abstract:
... A genuine biophysical method, Fourier transform-infrared (FT-IR) spectroscopy has become a versatile research tool in biochemistry and biomedicine. Topical applications in microbiology and prion research are impressive illustrations of the vigorous evolution of the technique. FT-IR spectroscopy has established itself as a powerful method for the rapid differentiation and identification of microorg ...
DOI:
10.1016/j.vetmic.2007.04.010