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- Matsubayashi, Makoto; Nagano, Saki; Kita, Toshimasa; Narushima, Tsuguto; Kimata, Isao; Iseki, Motohiro; Hajiri, Tomoya; Tani, Hiroyuki; Sasai, Kazumi; Baba, Eiichiroh
- Veterinary parasitology 2008 v.158 no.1-2 pp. 44-50
- microbial genetics; nucleotide sequences; Cryptosporidium andersoni; cattle; genotype; disease surveillance; feces; oocysts; microbial detection; Japan
- ... Previously, we reported that an isolate of novel type of Cryptosporidium andersoni detected in cattle in Japan contained Type A (identical to C. andersoni reported previously) and Type B (having a thymine nucleotide insertion unlike the Type A) genotypes in the 18S rRNA gene. Here, we conducted an extensive investigation of Cryptosporidium infections in adult cattle in Japan from 2004 to 2007. Con ...
- Bakheit, Mohammed A.; Torra, Dena; Palomino, Lily A.; Thekisoe, Oriel M.M.; Mbati, Peter A.; Ongerth, Jerry; Karanis, Panagiotis
- Veterinary parasitology 2008 v.158 no.1-2 pp. 11-22
- cattle diseases; Cryptosporidium parvum; oocysts; sheep; cattle; DNA; polymerase chain reaction; Cryptosporidium hominis; sheep diseases; feces; nucleotide sequences; cryptosporidiosis; microbial detection; horse diseases; horses; biogeography; product evaluation; South Africa
- ... Three LAMP (loop-mediated isothermal DNA amplification) assays were applied to detect Cryptosporidium species DNA in a total number of 270 fecal samples originating from cattle, sheep and horses in South Africa. DNA was extracted from 0.5g of fecal material. Results of LAMP detection were compared to those obtained by nested PCR targeting the Cryptosporidium 18 small subunit rRNA (18S) gene. All s ...