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- Author:
- Seale, Jessica; Swain, Josh; Booth, Richard; Blake, Damer P.
- Source:
- Veterinary parasitology 2019 v.273 pp. 32-35
- ISSN:
- 0304-4017
- Subject:
- Cryptosporidium parvum, etc ; calves; farms; feces; genotype; halofuginone; lactic acid; loci; polymerase chain reaction; ribosomal DNA; England; Show all 11 Subjects
- Abstract:
- ... This study looked to assess the stability of Cryptosporidium parvum genotypes in calves between the final day of treatment with the antiprotozoal halofuginone lactate and seven days post-treatment. Paired faecal samples were collected on the final day of treatment and seven days later from 54 calves across seven farms in South-west England. The presence of Cryptosporidium species was detected usin ...
- DOI:
- 10.1016/j.vetpar.2019.07.009
-
https://dx.doi.org/10.1016/j.vetpar.2019.07.009
- Author:
- Elguero, María E.; Tomazic, Mariela L.; Montes, María G.; Florin-Christensen, Mónica; Schnittger, Leonhard; Nusblat, Alejandro D.
- Source:
- Veterinary parasitology 2019 v.271 pp. 45-50
- ISSN:
- 0304-4017
- Subject:
- Cryptosporidium parvum, etc ; Protozoa; Tetrahymena thermophila; adhesion; antigens; body weight; calves; cryptosporidiosis; detergents; diarrhea; glycoproteins; heterologous gene expression; oocysts; parasites; recombinant vaccines; reticuloendothelial system; Show all 16 Subjects
- Abstract:
- ... Cryptosporidium parvum is a protozoan parasite of the phylum Apicomplexa responsible for cryptosporidiosis in calves, a disease that causes significant diarrhea and impairs gain of body weight, generating important production losses. As to now, no effective drugs or vaccines are available for the treatment or prevention of bovine cryptosporidiosis. Several reports suggest that development of a vac ...
- DOI:
- 10.1016/j.vetpar.2019.06.008
-
https://dx.doi.org/10.1016/j.vetpar.2019.06.008
- Author:
- Vélez, Juan; Lange, Malin K.; Zieger, Peter; Yoon, Ilkyu; Failing, Klaus; Bauer, Christian
- Source:
- Veterinary parasitology 2019 v.269 pp. 57-64
- ISSN:
- 0304-4017
- Subject:
- Cryptosporidium parvum, etc ; Saccharomyces cerevisiae; calves; cattle diseases; coproantigens; cryptosporidiosis; dairy farming; diarrhea; feces; females; fermented foods; halofuginone; milk; non-genetically modified foods; oocysts; weight gain; yeasts; Show all 17 Subjects
- Abstract:
- ... The objective of this study was to compare the effect of non-GMO Saccharomyces cerevisiae fermentation products (SCFP) with that of a halofuginone treatment against Cryptosporidium parvum infection in pre-weaned calves on a commercial dairy farm. A total of 123 neonatal female calves, housed in individual hutches, were enrolled sequentially based on date of birth in 41 blocks of 3 animals each. Ca ...
- DOI:
- 10.1016/j.vetpar.2019.04.008
-
https://dx.doi.org/10.1016/j.vetpar.2019.04.008
- Author:
- Gharieb, Rasha M.A.; Bowman, Dwight D.; Liotta, Janice L.; Xiao, Lihua
- Source:
- Veterinary parasitology 2019 v.271 pp. 80-86
- ISSN:
- 0304-4017
- Subject:
- Cryptosporidium parvum, etc ; DNA; amino acids; calves; dairy farming; farms; feces; genes; genotype; genotyping; glycoproteins; nucleotides; oocysts; polymerase chain reaction; restriction fragment length polymorphism; ribosomal RNA; sequence analysis; Finger Lakes region; New York; Show all 19 Subjects
- Abstract:
- ... The ability of the small-subunit ribosomal RNA (SSU rRNA) based nested PCR and Restriction Fragment Length Polymorphism (PCR-RFLP) to identify and genotype a single Cryptosporidium oocyst isolated from bovine faecal samples was evaluated in this study. In addition, subtyping was carried out by sequencing the 60 kDa glycoprotein (gp60) gene from the same single oocyst. Faecal samples were collected ...
- DOI:
- 10.1016/j.vetpar.2019.05.003
-
https://dx.doi.org/10.1016/j.vetpar.2019.05.003
- Author:
- Björkman, Camilla; von Brömssen, Claudia; Troell, Karin; Svensson, Catarina
- Source:
- Veterinary parasitology 2018 v.264 pp. 58-63
- ISSN:
- 0304-4017
- Subject:
- Cryptosporidium parvum, etc ; body condition; calcium hydroxide; calves; cleaning; cryptosporidiosis; dairy herds; diarrhea; disinfection; dominant species; farmers; farms; oocysts; veterinarians; Show all 14 Subjects
- Abstract:
- ... Diarrhea is common in young calves and is often caused by Cryptosporidium parvum infection. The aim of this study was to investigate if disinfection of calf pens with hydrated lime would reduce contamination of C. parvum oocysts and improve calf health in herds with C. parvum associated diarrhea problems. Four dairy herds with ongoing C. parvum associated calf diarrhea problems each participated i ...
- DOI:
- 10.1016/j.vetpar.2018.11.004
-
https://dx.doi.org/10.1016/j.vetpar.2018.11.004
- Author:
- Tomazic, M.L.; Rodriguez, A.E.; Lombardelli, J.; Poklepovich, T.; Garro, C.; Galarza, R.; Tiranti, K.; Florin-Christensen, M.; Schnittger, L.
- Source:
- Veterinary parasitology 2018 v.264 pp. 74-78
- ISSN:
- 0304-4017
- Subject:
- Cryptosporidium parvum, etc ; Internet; Protozoa; alleles; antibodies; antigens; bioinformatics; calves; cryptosporidiosis; diarrhea; oocysts; parasites; proteins; proteome; subunit vaccines; vaccination; vaccine development; Show all 17 Subjects
- Abstract:
- ... The apicomplexan protozoan Cryptosporidium parvum is an important causative agent of diarrhea of neonatal bovines. Vaccination has been proposed as an advantageous strategy against cryptosporidiosis of calves since besides protection against disease it has also the potential to prevent dissemination of infective oocysts into the environment. Antigens anchored to the parasite surface via glycosylph ...
- DOI:
- 10.1016/j.vetpar.2018.11.007
-
https://dx.doi.org/10.1016/j.vetpar.2018.11.007
- Author:
- Zhao, Guang-Hui; Gong, Ai-Yu; Wang, Yang; Zhang, Xin-Tian; Li, Min; Mathy, Nicholas W.; Chen, Xian-Ming
- Source:
- Veterinary parasitology 2018 v.251 pp. 27-33
- ISSN:
- 0304-4017
- Subject:
- Cryptosporidium parvum, etc ; Protozoa; cryptosporidiosis; epithelial cells; gene expression; gene expression regulation; genes; messenger RNA; methylation; models; parasites; transcription (genetics); zinc finger motif; Show all 13 Subjects
- Abstract:
- ... Intestinal infection by the zoonotic protozoan, Cryptosporidium parvum, causes significant alterations in the gene expression profile in host epithelial cells. The molecular mechanisms of how C. parvum may modulate host cell gene transcription and the pathological significance of such alterations are largely unclear. Previous studies demonstrate that a panel of parasite RNA transcripts are deliver ...
- DOI:
- 10.1016/j.vetpar.2017.12.015
-
https://dx.doi.org/10.1016/j.vetpar.2017.12.015
- Author:
- Dinler, Ceren; Ulutas, Bulent; Voyvoda, Huseyin; Ulutas, Pinar Alkim; Ural, Kerem; Karagenc, Tulin
- Source:
- Veterinary parasitology 2017 v.247 pp. 49-56
- ISSN:
- 0304-4017
- Subject:
- Cryptosporidium parvum, etc ; acute phase proteins; amyloid; blood serum; colostrum; feces; haptoglobins; lambs; models; oocysts; Show all 10 Subjects
- Abstract:
- ... This study aimed to evaluate the acute phase response (APR) through haptoglobin (Hp) and serum amyloid A (SAA) concentrations in serum and to examine the correlation between these acute phase proteins (APPs) and oocyst shedding using experimental Cryptosporidium parvum (C. parvum) infection model in neonatal lambs. Twenty lambs were divided into two equal groups: group CON remained uninfected as n ...
- DOI:
- 10.1016/j.vetpar.2017.09.023
-
https://dx.doi.org/10.1016/j.vetpar.2017.09.023
- Author:
- Azmanis, Panagiotis; di Somma, Antonio; Pappalardo, Lucia; Silvanose, Christu-Das; Bangoura, Berit
- Source:
- Veterinary parasitology 2018 v.252 pp. 167-172
- ISSN:
- 0304-4017
- Subject:
- Cryptosporidium parvum, etc ; Cryptosporidium baileyi; Falco rusticolus; air sacs; biochemistry; birds of prey; cryptosporidiosis; emerging diseases; endoscopy; falcons; feces; flight; fungal spores; genes; hematology; loci; lungs; parasitology; polymerase chain reaction; radiography; sequence analysis; Show all 21 Subjects
- Abstract:
- ... Cryptosporidiosis in raptors and falcons is well-known to be caused by Cryptosporidium baileyi and associated mainly with respiratory pathology. This report presents the diagnosis of an atypical cryptosporidiosis event caused by Cryptosporidium parvum, that to the authors’ knowledge, is a case observed for the first time in falcons. Two falcons (Gyrfalcon x Peregrine hybrids) were presented for an ...
- DOI:
- 10.1016/j.vetpar.2018.02.012
-
https://dx.doi.org/10.1016/j.vetpar.2018.02.012
- Author:
- Asadpour, Mohammad; Namazi, Fatemeh; Razavi, Seyed Mostafa; Nazifi, Saeed
- Source:
- Veterinary parasitology 2018 v.250 pp. 7-14
- ISSN:
- 0304-4017
- Subject:
- Cryptosporidium parvum, etc ; Protozoa; animal models; antioxidant activity; curcumin; dairy calves; digestive system diseases; drugs; females; genes; histopathology; hosts; ileum; jejunum; malondialdehyde; mice; oocysts; parasites; polymerase chain reaction; ribosomal RNA; sequence analysis; villi; Show all 22 Subjects
- Abstract:
- ... Cryptosporidium is a ubiquitous protozoan parasite causing gastrointestinal disorder in various hosts worldwide. The disease is self-limiting in the immunocompetent but life-threatening in immunodeficient individuals. Investigations to find an effective drug for the complete elimination of the Cryptosporidium infection are ongoing and urgently needed. The current study was undertaken to examine th ...
- DOI:
- 10.1016/j.vetpar.2017.12.008
-
http://dx.doi.org/10.1016/j.vetpar.2017.12.008
- Author:
- Pecková, Radka; Stuart, Peter D.; Sak, Bohumil; Květoňová, Dana; Kváč, Martin; Foitová, Ivona
- Source:
- Veterinary parasitology 2016 v.230 pp. 1-5
- ISSN:
- 0304-4017
- Subject:
- Cryptosporidium parvum, etc ; excystation; in vitro studies; oocysts; pathogenicity; permeability; protocols; sodium hypochlorite; sporozoites; trypsin; viability; Show all 11 Subjects
- Abstract:
- ... Excystation of sporozoites of Cryptosporidium parvum from oocysts is essential for successful in vitro assays. It has also been traditionally used as a measure for oocyst viability and infectivity. Laboratories use various excystation protocols so there is a need to clarify which method is the best. In this study, six different protocols for in vitro excystation of C. parvum oocysts were compared ...
- DOI:
- 10.1016/j.vetpar.2016.10.007
-
https://dx.doi.org/10.1016/j.vetpar.2016.10.007
- Author:
- Cai, Min; Guo, Yaqiong; Pan, Baoliang; Li, Na; Wang, Xiaolan; Tang, Chuanxiang; Feng, Yaoyu; Xiao, Lihua
- Source:
- Veterinary parasitology 2017 v.241 pp. 14-19
- ISSN:
- 0304-4017
- Subject:
- Cryptosporidium parvum, etc ; bulls; dairy calves; developed countries; diarrhea; farms; feces; genes; genotype; genotyping; glycoproteins; mixed infection; monitoring; restriction fragment length polymorphism; ribosomal RNA; sequence analysis; China; Show all 17 Subjects
- Abstract:
- ... In pre-weaned dairy calves, the zoonotic and pathogenic species Cryptosporidium parvum is the dominant Cryptosporidium species in most industrialized nations. In several studies in China, however, C. bovis has been the dominant one. To further examine the distribution of Cryptosporidium species in pre-weaned dairy calves in China, 818 fecal specimens were collected from five farms in Shanghai, wit ...
- DOI:
- 10.1016/j.vetpar.2017.05.005
-
https://dx.doi.org/10.1016/j.vetpar.2017.05.005
- Author:
- Inácio, Sandra Valéria; Widmer, Giovanni; de Brito, Roberta Lomonte Lemos; Zucatto, Anaiza Simão; de Aquino, Monally Conceição Costa; Oliveira, Bruno César Miranda; Nakamura, Alex Akira; Neto, Luiz da Silveira; Carvalho, João Gabriel Balizardo; Gomes, Jancarlo Ferreira; Meireles, Marcelo Vasconcelos; Bresciani, Katia Denise Saraiva
- Source:
- Veterinary parasitology 2017 v.233 pp. 48-51
- ISSN:
- 0304-4017
- Subject:
- Cryptosporidium parvum, etc ; Cryptosporidium hominis; DNA; actin; breeds; farms; feces; foals; genes; genotype; glycoproteins; humans; parasites; polymerase chain reaction; ribosomal RNA; Brazil; Show all 16 Subjects
- Abstract:
- ... The present study focuses on Cryptosporidium infections of foals in Brazil. A total of 92 animals of different breeds from 11 farms in the vicinity of Araçatuba in the state of São Paulo, were examined. According to PCR targeting the 18S rRNA gene, Cryptosporidium sp. DNA was detected in 21.7% (20/92) of foals. Good quality 18S rRNA, actin, HSP70 and gp60 genes nPCR amplicons were obtained from fi ...
- DOI:
- 10.1016/j.vetpar.2016.11.021
-
https://dx.doi.org/10.1016/j.vetpar.2016.11.021
- Author:
- Delling, Cora; Lendner, Matthias; Müller, Uwe; Daugschies, Arwid
- Source:
- Veterinary parasitology 2017 v.245 pp. 5-13
- ISSN:
- 0304-4017
- Subject:
- Cryptosporidium parvum, etc ; DNA; ambient temperature; chemical treatment; culture media; dimethyl sulfoxide; disinfectants; excystation; genes; hardness; hydrogen peroxide; in vitro studies; oocysts; parasites; quantitative polymerase chain reaction; sporozoites; toxicity; viability; washing; Show all 19 Subjects
- Abstract:
- ... Cryptosporidium parvum has been suggested as a suitable target for in vitro efficacy testing of disinfectants. To improve validity of a method based on exposure of HCT-8 monolayers to C. parvum oocysts we here critically evaluate and we propose certain procedural steps needed for the validation of disinfectants. Within a range of 0.02% to 0.4%, sodium taurocholate at 0.2% stimulated infection most ...
- DOI:
- 10.1016/j.vetpar.2017.07.018
-
https://dx.doi.org/10.1016/j.vetpar.2017.07.018
- Author:
- Oliveira, Bruno César Miranda; Ferrari, Elis Domingos; da Cruz Panegossi, Mariele Fernanda; Nakamura, Alex Akira; Corbucci, Flávio Sader; Nagata, Walter Bertequini; dos Santos, Bianca Martins; Gomes, Jancarlo Ferreira; Meireles, Marcelo Vasconcelos; Widmer, Giovanni; Bresciani, Katia Denise Saraiva
- Source:
- Veterinary parasitology 2017 v.243 pp. 148-150
- ISSN:
- 0304-4017
- Subject:
- Cryptosporidium parvum, etc ; Columba livia; breeding; cities; exhibitions; feces; females; genes; humans; malachite green; males; microscopy; oocysts; parasites; pigeons; polymerase chain reaction; public health; recreation; ribosomal DNA; ribosomal RNA; risk; Brazil; Show all 22 Subjects
- Abstract:
- ... The carrier pigeon and the domestic pigeon are different breeds of the species Columba livia. Carrier pigeons are used for recreational activities such as bird contests and exhibitions. Due to the close contact with humans, these birds may potentially represent a public health risk, since they can host and disseminate zoonotic parasites, such as those belonging to the genus Cryptosporidium (phylum ...
- DOI:
- 10.1016/j.vetpar.2017.06.023
-
https://dx.doi.org/10.1016/j.vetpar.2017.06.023
- Author:
- Zambriski, J.A.; Nydam, D.V.; Wilcox, Z.J.; Bowman, D.D.; Mohammed, H.O.; Liotta, J.L.
- Source:
- Veterinary parasitology 2013 v.197 no.1-2 pp. 104-112
- ISSN:
- 0304-4017
- Subject:
- Cryptosporidium parvum, etc ; cryptosporidiosis; dairy calves; diarrhea; feces; oocysts; Show all 6 Subjects
- Abstract:
- ... The objectives were to determine the median infective dose (ID50) of Cryptosporidium parvum and to describe the dose–response relationship including associated clinical illness in experimentally challenged dairy calves. Within the first 24h of life, 27 test calves were experimentally challenged with C. parvum oocysts and 3 control calves were sham dosed. Test calves received 1 of 8 possible doses ...
- DOI:
- 10.1016/j.vetpar.2013.04.022
- PubMed:
- 23680540
-
https://dx.doi.org/10.1016/j.vetpar.2013.04.022
- Author:
- Galuppi, R.; Piva, S.; Castagnetti, C.; Sarli, G.; Iacono, E.; Fioravanti, M.L.; Caffara, M.
- Source:
- Veterinary parasitology 2016 v.219 pp. 53-56
- ISSN:
- 0304-4017
- Subject:
- Cryptosporidium parvum, etc ; asphyxia; cryptosporidiosis; diarrhea; feces; foals; genes; glycoproteins; humans; mares; pain; polymerase chain reaction; restriction fragment length polymorphism; ribosomal DNA; sepsis (infection); staining; students; veterinarians; Show all 18 Subjects
- Abstract:
- ... This paper describes the transmission of a zoonotic subtype of Cryptosporidium parvum between two foals hospitalized in an Equine Perinatology Unit (EPU) linked to an outbreak of cryptosporidiosis in veterinary students. Fecal specimens of 36 mares (105 samples) and 28 foals (122 samples) were subjected to Ziehl–Neelsen staining, nested PCR of 18S rDNA. Two foals tested positive for Cryptosporidiu ...
- DOI:
- 10.1016/j.vetpar.2016.02.001
-
https://dx.doi.org/10.1016/j.vetpar.2016.02.001
- Author:
- Li, Fuhuang; Wang, Haiyan; Zhang, Zhenjie; Li, Junqiang; Wang, Chenrong; Zhao, Jinfeng; Hu, Suhui; Wang, Rongjun; Zhang, Longxian; Wang, Ming
- Source:
- Veterinary parasitology 2016 v.219 pp. 61-65
- ISSN:
- 0304-4017
- Subject:
- Cryptosporidium parvum, etc ; Cryptosporidium andersoni; DNA; Giardia lamblia; calves; dairy cattle; feces; genes; heifers; microscopy; nucleotide sequences; oocysts; ribosomal RNA; sequence analysis; triose-phosphate isomerase; China; Show all 16 Subjects
- Abstract:
- ... 822 fecal samples from cattle in six areas of Beijing were examined with microscopy for Cryptosporidium oocysts and Giardia cysts. The overall infection rates for Cryptosporidium spp. and Giardia duodenalis were 2.55% and 1.09%, respectively. Cryptosporidium was only detected in calves and heifers, whereas G. duodenalis was found in all age groups. Cryptosporidium spp. were characterized with a PC ...
- DOI:
- 10.1016/j.vetpar.2016.01.023
-
https://dx.doi.org/10.1016/j.vetpar.2016.01.023
- Author:
- Mirhashemi, Marzieh Ezzaty; Zintl, Annetta; Grant, Tim; Lucy, Frances; Mulcahy, Grace; Waal, Theo De
- Source:
- Veterinary parasitology 2016 v.216 pp. 18-22
- ISSN:
- 0304-4017
- Subject:
- Cryptosporidium parvum, etc ; Cryptosporidium andersoni; Cryptosporidium bovis; Cryptosporidium ryanae; Protozoa; cattle; cryptosporidiosis; diarrhea; farms; genotype; horses; hosts; molecular epidemiology; sheep; spring; summer; swine; watersheds; Ireland; Show all 19 Subjects
- Abstract:
- ... Cryptosporidium is a protozoan that can cause gastro-intestinal illness with diarrhoea in a wide range of hosts. In fact some species of Cryptosporidium can infect the broad range of hosts. The current paper is focused to investigate monthly prevalence and diversity of Cryptosporidium spp. during the spring and early summer (March–June) in 2009 and 2010 in farms with no history of cryptosporidiosi ...
- DOI:
- 10.1016/j.vetpar.2015.12.002
-
https://dx.doi.org/10.1016/j.vetpar.2015.12.002
- Author:
- Jacobson, Caroline; Williams, Andrew; Yang, Rongchang; Ryan, Una; Carmichael, Ian; Campbell, Angus J.; Gardner, Graham E.
- Source:
- Veterinary parasitology 2016 v.228 pp. 42-51
- ISSN:
- 0304-4017
- Subject:
- Cryptosporidium parvum, etc ; Giardia; Haemonchus; body weight; carcass weight; eggs; farms; feces; genetic background; grazing; lambs; linear models; longitudinal studies; meat production; oocysts; parasites; quantitative polymerase chain reaction; sheep meat; slaughter; weaning; Show all 20 Subjects
- Abstract:
- ... Associations between intensity and frequency of Cryptosporidium and Giardia shedding with growth, carcase weight and dressing% were investigated using a longitudinal study of 1182 lambs on eight Australian farms. Live weight was recorded and faecal samples were collected on three sampling occasions; weaning (approximately 12 weeks of age), post-weaning (approximately 19 weeks) and pre-slaughter (a ...
- DOI:
- 10.1016/j.vetpar.2016.08.003
-
https://dx.doi.org/10.1016/j.vetpar.2016.08.003