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- Chrzastek, Klaudia; Smith, Diane; Sharma, Poonam; Suarez, David L.; Pantin-Jackwood, Mary; Kapczynski, Darrell R.; Lee, Dong-hun
- Virology 2017 v.509 pp. 159-166
- Infectious bronchitis virus; Influenza A virus; Newcastle disease virus; RNA; birds; genome; genome assembly; high-throughput nucleotide sequencing; metagenomics; microbial detection; nucleotide sequences; rapid methods; viruses
- ... Current technologies with next generation sequencing have revolutionized metagenomics analysis of clinical samples. To achieve the non-selective amplification and recovery of low abundance genetic sequences, a simplified Sequence-Independent, Single-Primer Amplification (SISPA) technique in combination with MiSeq platform was applied to target negative- and positive-sense single-stranded RNA viral ...