PubAg

Main content area

You searched for: Journal Virus genes Remove constraint Journal: Virus genes Publication Year 2016 Remove constraint Publication Year: 2016 Source 2016 v.52 no.6 Remove constraint Source: 2016 v.52 no.6
Start Over

Search

22  Search Results

« Previous | 1 - 20 of 22 |
Number of results to display per page

Search Results

1. Amino acid mutations in the env gp90 protein that modify N-linked glycosylation of the Chinese EIAV vaccine strain enhance resistance to neutralizing antibodies

Author:
Han, Xiue; Zhang, Ping; Yu, Wei; Xiang, Wenhua; Li, Xiaodong
Source:
Virus genes 2016 v.52 no.6 pp. 814-822
ISSN:
0920-8569
Subject:
amino acid substitution; amino acids; asses; blood serum; genes; glycosylation; horses; live vaccines; neutralization; neutralizing antibodies; site-directed mutagenesis; virulence; viruses
Abstract:
... The Chinese EIAV vaccine is an attenuated live virus vaccine obtained by serial passage of a virulent horse isolate (EIAVL) in donkeys (EIAVD) and, subsequently, in donkey cells in vitro. In this study, we compare the env gene of the original horse virulent virus (EIAVL) with attenuated strains serially passaged in donkey MDM (EIAVDLV) and donkey dermal cells (EIAVFDDV). Genetic comparisons among ...
DOI:
10.1007/s11262-016-1382-2

2. A novel bocavirus from domestic mink, China

Author:
Yang, Shixing; Wang, Yan; Li, Wang; Fan, Zhaobin; Jiang, Lili; Lin, Yuan; Fu, Xingli; Shen, Quan; Sun, Zixuan; Wang, Xiaochun; Deng, Xutao; Zhang, Wen; Delwart, Eric
Source:
Virus genes 2016 v.52 no.6 pp. 887-890
ISSN:
0920-8569
Subject:
diarrhea; genome; polymerase chain reaction; phylogeny; animal health; amino acid sequences; feces; mink; viruses; viral shedding; carnivores; Bocaparvovirus; China
Abstract:
... Bocaviruses have been found in the feces of humans and a variety of animals, including pigs, cattle, dogs, gorillas, cats, and sea lions. Here, we have characterized the almost complete genome (5224 nt) of a novel bocavirus from feces of domestic minks, which has been provisionally named mink bocavirus. The NS1 protein of mink bocavirus shared 36.9–52 % amino acid sequence identities with those of ...
DOI:
10.1007/s11262-016-1380-4

3. A novel fusarivirus isolated from the phytopathogenic fungus Nigrospora oryzae

Author:
Zhong, Jie; Zhao, Si Qi; Li, Gui Fang; Pang, Xi Dan; Deng, Xiao Jun; Zhu, Hong Jian; Da Gao, Bi; Zhou, Qian
Source:
Virus genes 2016 v.52 no.6 pp. 891-895
ISSN:
0920-8569
Subject:
Khuskia oryzae; RNA replication; RNA-directed RNA polymerase; amino acids; genome; mycoviruses; open reading frames; phylogeny; plant pathogenic fungi; polypeptides; proteins; sequence homology
Abstract:
... In this study, we report the discovery and molecular characterization of a novel mycovirus, Nigrospora oryzae fusarivirus 1 (NoFV1) isolated from the rice-infecting fungus Nigrospora oryzae. Excluding a poly (A) tail, the genome of the virus is 7004 nucleotide (nt) long containing three putative nonoverlapping open reading frames (ORF1, ORF2, and ORF3). The large ORF1 encodes a polypeptide with a ...
DOI:
10.1007/s11262-016-1371-5

4. Characterization of Shuni viruses detected in Israel

Author:
Golender, Natalia; Wernike, Kerstin; Bumbarov, Velizar; Aebischer, Andrea; Panshin, Alexander; Jenckel, Maria; Khinich, Yevgeny; Beer, Martin
Source:
Virus genes 2016 v.52 no.6 pp. 806-813
ISSN:
0920-8569
Subject:
stop codon; interferon-alpha; Shuni orthobunyavirus; viral nonstructural proteins; cattle; brain; goats; mutation; sheep; bioassays; fetus; viruses; neonates; nucleocapsid proteins; Israel
Abstract:
... Shuni virus (SHUV) was recently identified in Israel in several brains of ovine, bovine, and goat fetuses and newborn animals with congenital arthrogryposis-hydranencephaly syndrome. In the present study, the sequences of several Israeli SHUV strains were analyzed in detail; based on the small genome segment which encodes the nucleocapsid protein and the small nonstructural protein (NSs), a very h ...
DOI:
10.1007/s11262-016-1381-3

5. Complete nucleotide sequences of dsRNA2 and dsRNA7 detected in the phytopathogenic fungus Sclerotium hydrophilum and their close phylogenetic relationship to a group of unclassified viruses

Author:
Wang, Chunlan; Wu, Juan; Zhu, Xiwu; Chen, Jishuan
Source:
Virus genes 2016 v.52 no.6 pp. 823-827
ISSN:
0920-8569
Subject:
Cryphonectria parasitica; Curvularia; Lactarius rufus; Penicillium aurantiogriseum; RNA viruses; RNA-directed RNA polymerase; Sclerotium hydrophilum; double-stranded RNA; heat tolerance; molecular cloning; mycelium; mycoviruses; open reading frames; phylogeny; plant pathogenic fungi; proteins; sequence analysis; unassigned viruses; virus-like particles
Abstract:
... Ten dsRNA segments were extracted from Sclerotium hydrophilum isolate (HZ11). The isolation of virus-like particles contained 10 dsRNA segments with the same number and migration as those extracted directly from the fungal mycelia. Two of these dsRNA segments, dsRNA2 and dsRNA7, were cloned and sequenced. They were 2121 and 1953 bp, respectively. The dsRNA2 encodes a RNA-dependent RNA polymerase. ...
DOI:
10.1007/s11262-016-1375-1

6. Detection of novel ferret coronaviruses and evidence of recombination among ferret coronaviruses

Author:
Minami, Shohei; Kuroda, Yudai; Terada, Yutaka; Yonemitsu, Kenzo; Van Nguyen, Dung; Kuwata, Ryusei; Shimoda, Hiroshi; Takano, Ai; Maeda, Ken
Source:
Virus genes 2016 v.52 no.6 pp. 858-862
ISSN:
0920-8569
Subject:
Feline coronavirus; RNA-directed RNA polymerase; epidemiological studies; ferrets; genes; nucleocapsid; open reading frames; pathogenesis; phylogeny; reverse transcriptase polymerase chain reaction; sequence analysis; transcription (genetics)
Abstract:
... In an epidemiological study of ferret coronaviruses (FRCoVs), novel FRCoV strains (Saitama-1 and Aichi-1) were detected by reverse transcription-polymerase chain reaction (RT-PCR) and nucleotide sequence analysis of partial RNA-dependent RNA polymerase (RdRp) genes. Phylogenetic analysis indicated that these strains belonged to different clusters from other FRCoV strains. Next, the nucleotide sequ ...
DOI:
10.1007/s11262-016-1365-3

7. Epidemiological investigation of Middle East respiratory syndrome coronavirus in dromedary camel farms linked with human infection in Abu Dhabi Emirate, United Arab Emirates

Author:
Muhairi, Salama Al; Hosani, Farida Al; Eltahir, Yassir M.; Mulla, Mariam Al; Yusof, Mohammed F.; Serhan, Wissam S.; Hashem, Farouq M.; Elsayed, Elsaeid A.; Marzoug, Bahaaeldin A.; Abdelazim, Assem S.
Source:
Virus genes 2016 v.52 no.6 pp. 848-854
ISSN:
0920-8569
Subject:
nose; genome; phylogeny; Camelus dromedarius; livestock and meat industry; open reading frames; farms; reverse transcription; viruses; viral shedding; screening; epidemiological studies; humans; calves; Middle East respiratory syndrome coronavirus; quantitative polymerase chain reaction; zoonoses; virus transmission; camels; sheep; reverse transcriptase polymerase chain reaction; United Arab Emirates
Abstract:
... The objective of this research was to investigate the prevalence of Middle East respiratory syndrome coronavirus (MERS-CoV) infection primarily in dromedary camel farms and the relationship of those infections with infections in humans in the Emirate of Abu Dhabi. Nasal swabs from 1113 dromedary camels (39 farms) and 34 sheep (1 farm) and sputum samples from 2 MERS-CoV-infected camel farm owners a ...
DOI:
10.1007/s11262-016-1367-1

8. Evidence of the presence of the Zika virus in Mexico since early 2015

Author:
Díaz-Quiñonez, José Alberto; López-Martínez, Irma; Torres-Longoria, Belem; Vázquez-Pichardo, Mauricio; Cruz-Ramírez, Edith; Ramírez-González, José Ernesto; Ruiz-Matus, Cuitláhuac; Kuri-Morales, Pablo
Source:
Virus genes 2016 v.52 no.6 pp. 855-857
ISSN:
0920-8569
Subject:
Aedes aegypti; RNA; Zika virus; dengue; public health; reverse transcriptase polymerase chain reaction; viruses; Mexico
Abstract:
... To assess the possible circulation of Zika virus (ZIKV) prior to the first documented case in Mexico, we reanalyzed the stored samples from the states of Veracruz and Yucatán, which were originally collected to test for dengue (DENV) and chikungunya (CHIKV) but were negative for these viruses despite the symptomatology. The samples were originally collected between the 30 and 46 epidemiological we ...
DOI:
10.1007/s11262-016-1384-0

9. Genetic and molecular characterization of a novel reassortant H2N8 subtype avian influenza virus isolated from a domestic duck in Zhejiang Province in China

Author:
Wu, Haibo; Peng, Xiuming; Peng, Xiaorong; Cheng, Linfang; Wu, Nanping
Source:
Virus genes 2016 v.52 no.6 pp. 863-866
ISSN:
0920-8569
Subject:
Influenza A virus; ducks; lungs; mice; monitoring; pathogenicity; phylogeny; risk; viruses; water birds; China
Abstract:
... The circulation of the H2 subtype influenza viruses in domestic animals increases the risk of human exposure to these viruses. An H2N8 avian influenza virus (AIV) was isolated from a domestic duck during AIV surveillance of poultry in live poultry markets (LPMs) in Zhejiang Province, Eastern China, in 2013. The phylogenetic trees suggested that this strain is a novel reassortant virus derived from ...
DOI:
10.1007/s11262-016-1368-0

10. Genetic characterization of highly pathogenic avian influenza H5N1 viruses isolated from naturally infected pigeons in Egypt

Author:
Elgendy, Emad Mohamed; Watanabe, Yohei; Daidoji, Tomo; Arai, Yasuha; Ikuta, Kazuyoshi; Ibrahim, Madiha Salah; Nakaya, Takaaki
Source:
Virus genes 2016 v.52 no.6 pp. 867-871
ISSN:
0920-8569
Subject:
Influenza A virus; avian influenza; chickens; ducks; farms; genes; genetic techniques and protocols; humans; morbidity; mortality; mutation; nucleoproteins; phylogeny; pigeons; public health; risk; viral nonstructural proteins; virus transmission; viruses; Egypt
Abstract:
... Avian influenza viruses impose serious public health burdens with significant mortality and morbidity not only in poultry but also in humans. While poultry susceptibility to avian influenza virus infection is well characterized, pigeons have been thought to have low susceptibility to these viruses. However, recent studies reported natural pigeon infections with highly pathogenic avian influenza H5 ...
DOI:
10.1007/s11262-016-1369-z

11. Genome sequence analysis of two South African isolates of Moroccan watermelon mosaic virus infecting cucurbits

Author:
Ibaba, Jacques Davy; Laing, Mark D.; Gubba, Augustine
Source:
Virus genes 2016 v.52 no.6 pp. 896-899
ISSN:
0920-8569
Subject:
Cucurbita pepo; Moroccan watermelon mosaic virus; amino acid sequences; genetic distance; genome; high-throughput nucleotide sequencing; nucleotide sequences; nucleotides; phylogeny; plant diseases and disorders; plant viruses; sequence homology; viruses; South Africa
Abstract:
... Moroccan watermelon mosaic virus (MWMV) has been prevalent in cucurbits in the Republic of South Africa (RSA) since it was first reported in 1987. However, full genome studies of the South African isolates have never been conducted previously. The full genome of two MWMV isolates infecting cucurbits (Cucurbita pepo L.) in the province of KwaZulu-Natal, RSA, was compared with the genome of the Tuni ...
DOI:
10.1007/s11262-016-1372-4

12. Grass carp reovirus NS26 interacts with cellular lipopolysaccharide-induced tumor necrosis factor-alpha factor, LITAF

Author:
Lu, Jianfei; Wang, Hao; Zhang, Yanan; Li, Yan; Lu, Liqun
Source:
Virus genes 2016 v.52 no.6 pp. 789-796
ISSN:
0920-8569
Subject:
Ctenopharyngodon idella; Escherichia coli; Reoviridae; double-stranded RNA; kidney cells; pathogenesis; precipitin tests; transfection; tumor necrosis factor-alpha; two hybrid system techniques; viral nonstructural proteins
Abstract:
... The nonstructural protein NS26 of grass carp reovirus (GCRV) is encoded by the 11th genomic dsRNA segment, homolog of which is not found in orthoreoviruses. The role of NS26 in GCRV pathogenesis is still unclear. Previously, grass carp LITAF/SIMPLE protein was identified as a putative binding partner for NS26 in a yeast two-hybrid screen. Here, we further characterized the association between NS26 ...
DOI:
10.1007/s11262-016-1370-6

13. Influenza A Virus-induced expression of ISG20 inhibits viral replication by interacting with nucleoprotein

Author:
Qu, Hongren; Li, Jing; Yang, Limin; Sun, Lei; Liu, Wenjun; He, Hongxuan
Source:
Virus genes 2016 v.52 no.6 pp. 759-767
ISSN:
0920-8569
Subject:
Influenza A virus; antiviral agents; antiviral properties; genes; hosts; humans; influenza; mutants; nucleoproteins; pathogens; progeny; protein synthesis; transcription (genetics); viral load; virus replication
Abstract:
... Influenza A virus (IAV) is an important pathogen that has a wide range of hosts and represents a threat to the health of humans and several animal species. IAV infection can induce the transcription of many genes in the host. In the present study, we demonstrated for the first time that three different strains of H1N1 IAV induce the expression of an IFN-stimulated gene, ISG20. We determined the an ...
DOI:
10.1007/s11262-016-1366-2

14. Low genetic diversity of Banana bunchy top virus, with a sub-regional pattern of variation, in Democratic Republic of Congo

Author:
Mukwa, L. F. T.; Gillis, A.; Vanhese, V.; Romay, G.; Galzi, S.; Laboureau, N.; Kalonji-Mbuyi, A.; Iskra-Caruana, M. L.; Bragard, C.
Source:
Virus genes 2016 v.52 no.6 pp. 900-905
ISSN:
0920-8569
Subject:
Banana bunchy top virus; bananas; crops; databases; food security; genetic variation; genome; haplotypes; household income; phylogeny; plantains (fruit); sequence analysis; viruses; Democratic Republic of the Congo
Abstract:
... Banana bunchy top virus (BBTV), belonging to the genus Babuvirus, is the most devastating and widespread banana virus. Banana and plantain are major crops in terms of household income and food security in Democratic Republic of Congo (DRC). Despite the large area under banana and plantain cultivation in the country, before this study, the genetic characterization of BBTV isolates had only been und ...
DOI:
10.1007/s11262-016-1383-1

15. Multiple virus infection in a single strain of Fusarium poae shown by deep sequencing

Author:
Osaki, Hideki; Sasaki, Atsuko; Nomiyama, Koji; Tomioka, Keisuke
Source:
Virus genes 2016 v.52 no.6 pp. 835-847
ISSN:
0920-8569
Subject:
Aspergillus foetidus; Fusarium poae; Narnaviridae; Ophiovirus; Partitiviridae; Phlebovirus; Sclerotinia sclerotiorum; amino acid sequences; double-stranded RNA; electrophoresis; genome; high-throughput nucleotide sequencing; mycoviruses; phylogeny; reverse transcriptase polymerase chain reaction; wheat
Abstract:
... Many bands were detected on an electrophoretic profile of double-stranded (ds) RNA preparation from a single strain of Fusarium poae isolated from wheat. When the purified dsRNA sample was deep-sequenced by a next-generation sequencer, sixteen virus-like assembled contigs with predicted amino acid sequences showing homologies to respective viral RNA-dependent RNA polymerases (RdRps) were found by ...
DOI:
10.1007/s11262-016-1379-x

16. Porcine bocavirus NP1 protein suppresses type I IFN production by interfering with IRF3 DNA-binding activity

Author:
Zhang, Ruoxi; Fang, Liurong; Wu, Wei; Zhao, Fuwei; Song, Tao; Xie, Lilan; Li, Yi; Chen, Huanchun; Xiao, Shaobo
Source:
Virus genes 2016 v.52 no.6 pp. 797-805
ISSN:
0920-8569
Subject:
transcription factors; immune evasion; viral nonstructural proteins; antagonists; precipitin tests; swine; Ungulate protoparvovirus 1; genes; viruses; chromatin; DNA-binding domains; phosphorylation; interferon-beta; Bocaparvovirus; defense mechanisms
Abstract:
... Type I interferon (IFN) and the IFN-induced cellular antiviral responses are the primary defense mechanisms against viral infection; however, viruses always evolve various mechanisms to antagonize this host’s IFN responses. Porcine bocavirus (PBoV) is a newly identified porcine parvovirus. In this study, we found that the nonstructural protein NP1 of PBoV inhibits Sendai virus-induced IFN-β produc ...
DOI:
10.1007/s11262-016-1377-z

17. Rapid detection of highly pathogenic porcine reproductive and respiratory syndrome virus by a fluorescent probe-based isothermal recombinase polymerase amplification assay

Author:
Yang, Yang; Qin, Xiaodong; Sun, Yingjun; Chen, Ting; Zhang, Zhidong
Source:
Virus genes 2016 v.52 no.6 pp. 883-886
ISSN:
0920-8569
Subject:
quantitative polymerase chain reaction; cross reaction; Suid alphaherpesvirus 1; Porcine reproductive and respiratory syndrome virus; detection limit; Classical swine fever virus; fluorescence; reverse transcriptase polymerase chain reaction; rapid methods; reverse transcription; Foot-and-mouth disease virus; RNA
Abstract:
... A novel fluorescent probe-based real-time reverse transcription recombinase polymerase amplification (real-time RT-RPA) assay was developed for rapid detection of highly pathogenic type 2 porcine reproductive and respiratory syndrome virus (HP-PRRSV). The sensitivity analysis showed that the detection limit of RPA was 70 copies of HP-PRRSV RNA/reaction. The real-time RT-RPA highly specific amplifi ...
DOI:
10.1007/s11262-016-1378-y

18. Review of Beet pseudoyellows virus genome structure built the consensus genome organization of cucumber strains and highlighted the unique feature of strawberry strain

Author:
Akhter, Md. Shamim; Bhor, Sachin Ashok; Hlalele, Ngcebo; Nao, Masahiro; Sekine, Ken-Taro; Yaeno, Takashi; Yamaoka, Naoto; Nishiguchi, Masamichi; Gubba, Augustine; Kobayashi, Kappei
Source:
Virus genes 2016 v.52 no.6 pp. 828-834
ISSN:
0920-8569
Subject:
Beet pseudoyellows virus; RNA; RNA-directed RNA polymerase; cucumbers; genes; open reading frames; phylogeny; sequence analysis; strawberries; Japan; South Africa
Abstract:
... The complete nucleotide sequences of Beet pseudoyellows virus (BPYV)-MI (cucumber isolate; Matsuyama, Idai) genomic RNAs 1 and 2 were determined and compared with the previously sequenced Japanese cucumber strain (BPYV-JC) and a strawberry strain (BPYV-S). The RNA 2 of BPYV-MI showed 99 % nucleotide sequence identity with both BPYV-JC and -S having highly conserved eight ORFs. In contrast, the RNA ...
DOI:
10.1007/s11262-016-1376-0

19. Short communication: antiviral activity of porcine IFN-λ3 against porcine epidemic diarrhea virus in vitro

Author:
Shen, Haiyan; Zhang, Chunhong; Guo, Pengju; Liu, Zhicheng; Sun, Minhua; Sun, Junying; Li, Linlin; Dong, Jiawen; Zhang, Jianfeng
Source:
Virus genes 2016 v.52 no.6 pp. 877-882
ISSN:
0920-8569
Subject:
Escherichia coli; Homo sapiens; Porcine epidemic diarrhea virus; Tupaia; amino acids; antiviral properties; cattle; cell culture; complementary DNA; diarrhea; genes; horses; interferon-gamma; molecular cloning; nucleotide sequences; phylogeny; sequence alignment; sequence homology; sheep; signal peptide; swine; viruses
Abstract:
... A new family of IFNs called type III IFN or IFN-λ has been described, and shown to induce antiviral activity against several viruses in the cell culture. In this study, the molecular cloning, expression, and antiporcine epidemic diarrhea virus (PEDV) activity of porcine IFN-λ3 (poIFN-λ3) were reported. The full-length poIFN-λ3 cDNA sequence encoded 196 amino acids with a 23 amino acid signal pepti ...
DOI:
10.1007/s11262-016-1374-2

20. The 2A2 protein of Duck hepatitis A virus type 1 induces apoptosis in primary cell culture

Author:
Cao, Jingyu; Ou, Xumin; Zhu, Dekang; Ma, Guangpeng; Cheng, Anchun; Wang, Mingshu; Chen, Shun; Jia, Renyong; Liu, Mafeng; Sun, Kunfeng; Yang, Qiao; Wu, Ying; Chen, Xiaoyue
Source:
Virus genes 2016 v.52 no.6 pp. 780-788
ISSN:
0920-8569
Subject:
Duck hepatitis A virus; apoptosis; cell culture; chromatin; flow cytometry; fluorescein; fluorescence microscopy; models; pathogenesis; plasmids; sequence alignment; serotypes; staining; transfection; viral proteins
Abstract:
... Duck hepatitis A virus type 1, (DHAV-1) 2A2ᵖʳᵒ, is one of the most highly conserved viral proteins within the DHAV serotypes. However, its effect on host cells is unclear. We predicted that DHAV-1 2A2ᵖʳᵒ was a GTPase-like protein based on the results of multiple sequence alignment and homologous modeling analysis. Upon transfection of a recombinant plasmid expressing DHAV-1 2A2, cells displayed fr ...
DOI:
10.1007/s11262-016-1364-4