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- Author:
- Ito, Takao; Sato, Akihiko; Suzaki, Koichi
- Source:
- Virus genes 2015 v.51 no.1 pp. 105-111
- ISSN:
- 0920-8569
- Subject:
- Closterovirus; Diospyros virginiana; RNA-directed RNA polymerase; amino acid sequences; coat proteins; genome; heat-shock protein 70; high-throughput nucleotide sequencing; host plants; leaves; nucleic acids; nucleotides; open reading frames; persimmons; phylogeny; viruses
- Abstract:
- ... Deep-sequencing analysis of nucleic acids extracted from leaf tissue of an American persimmon (Diospyros virginiana L.) and subsequent-sequencing analyses uncovered at least four distinct closterovirus-like molecules. Two complete genomes of 18,569 and 18,030 nucleotides (nt) and partial genomes of 4,899 and 9,019 nt were determined. The two complete genomes encoded 11 potential open reading frame ...
- DOI:
- 10.1007/s11262-015-1202-0
-
http://dx.doi.org/10.1007/s11262-015-1202-0
- Author:
- Phan, Tung Gia; da Costa, Antonio Charlys; Zhang, Wen; Pothier, Pierre; Ambert-Balay, Katia; Deng, Xutao; Delwart, Eric
- Source:
- Virus genes 2015 v.51 no.1 pp. 132-135
- ISSN:
- 0920-8569
- Subject:
- Gyrovirus; adults; chickens; diarrhea; feces; genome; humans; open reading frames; structural proteins; vertebrate viruses; viral nonstructural proteins
- Abstract:
- ... A novel gyrovirus genome found in the feces of an adult with diarrhea is described. The genome shows the three expected main ORFs encoding a structural protein (VP1), nonstructural protein (VP2), and Apoptin protein (VP3), which shared identities of 41, 42, and 38 % with those of the most closely related gyrovirus proteins, respectively. Given the high divergence in its genome, this gyrovirus may ...
- DOI:
- 10.1007/s11262-015-1210-0
- PubMed:
- 26013257
- PubMed Central:
- PMC4519424
-
http://dx.doi.org/10.1007/s11262-015-1210-0
- Author:
- Zhu, Hong Jian; Chen, Dan; Zhong, Jie; Zhang, Shuai Yang; Gao, Bi Da
- Source:
- Virus genes 2015 v.51 no.1 pp. 159-162
- ISSN:
- 0920-8569
- Subject:
- Alternaria longipes; Partitiviridae; Ustilaginoidea virens; double-stranded RNA; false smut; genome; mycoviruses; open reading frames; phylogeny; plant pathogenic fungi; rice; sequence homology; unassigned viruses
- Abstract:
- ... The complete sequence of a novel mycovirus infecting Ustilaginoidea virens, the causal agent of false smut of rice, is reported here and designated as Ustilaginoidea virens unassigned RNA virus HNND-1 (UvURV-HNND-1). This virus has an undivided dsRNA genome of 2903 nt in length and contains two non-overlapping open reading frames (ORF1 and 2), with the small ORF1 encoding a protein of unknown func ...
- DOI:
- 10.1007/s11262-015-1212-y
-
http://dx.doi.org/10.1007/s11262-015-1212-y
- Author:
- Almeyda, C. V.; Raikhy, G.; Pappu, H. R.
- Source:
- Virus genes 2015 v.51 no.1 pp. 96-104
- ISSN:
- 0920-8569
- Subject:
- Agrobacterium radiobacter; Dahlia mosaic virus; Dahlia pinnata; Nicotiana benthamiana; TATA box; agroinfiltration; beta-glucuronidase; electroporation; gene expression; leaves; reporter genes; viruses
- Abstract:
- ... Two distinct caulimoviruses, Dahlia mosaic virus (DMV) and Dahlia common mosaic virus (DCMV), and an endogenous plant pararetroviral sequence (DvEPRS, formerly known as DMV-D10) were reported from dahlia (Dahlia spp). Promoter elements from these dahlia-associated pararetroviruses were identified and characterized. The TATA box, the CAAT box, the transcription start site, the polyadenylation signa ...
- DOI:
- 10.1007/s11262-015-1196-7
-
http://dx.doi.org/10.1007/s11262-015-1196-7
- Author:
- Botka, Tibor; Růžičková, Vladislava; Konečná, Hana; Pantůček, Roman; Rychlík, Ivan; Zdráhal, Zbyněk; Petráš, Petr; Doškař, Jiří
- Source:
- Virus genes 2015 v.51 no.1 pp. 122-131
- ISSN:
- 0920-8569
- Subject:
- Siphoviridae; Staphylococcus aureus; bacteriophages; genes; hospitals; open reading frames; pathogenicity; phenotype; sequence homology; toxicity; virion
- Abstract:
- ... Exfoliative toxin A (ETA)-coding temperate bacteriophages are leading contributors to the toxic phenotype of impetigo strains of Staphylococcus aureus. Two distinct eta gene-positive bacteriophages isolated from S. aureus strains which recently caused massive outbreaks of pemphigus neonatorum in Czech maternity hospitals were characterized. The phages, designated ϕB166 and ϕB236, were able to tran ...
- DOI:
- 10.1007/s11262-015-1223-8
-
http://dx.doi.org/10.1007/s11262-015-1223-8
- Author:
- Zhao, Fumei; Hwang, Un Sun; Lim, Seungmo; Yoo, Ran Hee; Igori, Davaajargal; Lee, Su-Heon; Lim, Hyoun-Sub; Moon, Jae Sun
- Source:
- Virus genes 2015 v.51 no.1 pp. 163-166
- ISSN:
- 0920-8569
- Subject:
- Arabidopsis; Cauliflower mosaic virus; Glycine max; Nicotiana benthamiana; RNA; Tobacco ringspot virus; Western blotting; blight; clones; complementary DNA; ecotypes; genes; growth retardation; leaves; necrosis; pathogens; promoter regions; sap; soybeans; tomatoes; transmission electron microscopy
- Abstract:
- ... Tobacco ringspot virus (TRSV, genus Nepovirus), causes severe diseases in soybean and tobacco plants. TRSV-induced bud blight disease significantly reduced both the yield and quality of soybeans. The function of the encoded viral gene product involved in TRSV infection was unclear due to the limitation of reverse genetics studies on the viral genome. Here, we represent the successful construction ...
- DOI:
- 10.1007/s11262-015-1221-x
-
http://dx.doi.org/10.1007/s11262-015-1221-x
- Author:
- Yamamoto, Eiji; Ito, Hiroshi; Kitamoto, Eiji; Morinishi, Keiko; Yano, Atsushi; Miyoshi, Satomi; Ito, Toshihiro
- Source:
- Virus genes 2015 v.51 no.1 pp. 140-143
- ISSN:
- 0920-8569
- Subject:
- Pigeon circovirus; diarrhea; genetic techniques and protocols; genetic variation; genome; open reading frames; phylogeny; racing pigeons; Japan
- Abstract:
- ... We report the genetic analysis of a pigeon circovirus (PiCV/Japan/2/2010) detected in a racing pigeon with diarrheal symptoms in western Japan. Sequencing revealed a genome size of 2,041 bp that contained 3 open reading frames (ORFs), designated V1, C1, and C2, a structure consistent with to those of other pigeon circoviruses. Phylogenetic analyses of the ORF C1 revealed that PiCV/Japan/2/2010 div ...
- DOI:
- 10.1007/s11262-015-1211-z
-
http://dx.doi.org/10.1007/s11262-015-1211-z
- Author:
- Santhamani, Ramasamy; Venkatesan, Gnanavel; Minhas, Sanjeevna Kumari; Shivachandra, Sathish Bhadravati; Muthuchelvan, Dhanavelu; Pandey, Awadh Bihari; Ramakrishnan, Muthannan Andavar
- Source:
- Virus genes 2015 v.51 no.1 pp. 33-38
- ISSN:
- 0920-8569
- Subject:
- genes; goats; host specificity; open reading frames; phylogeny; polymerase chain reaction; sheep; sheep scab; vaccines; viruses; India
- Abstract:
- ... Recent developments in molecular biology shed light on cross-species transmission of SPPV and GTPV. The present study was planned to characterize the capripoxviruses which were circulating in the field condition among sheep and goats using RPO30 gene-based viral lineage (SPPV/GTPV) differentiating PCR and sequencing of RPO30 and GPCR genes from clinical samples. Out of 58 scabs (35 sheep and 23 go ...
- DOI:
- 10.1007/s11262-015-1206-9
-
http://dx.doi.org/10.1007/s11262-015-1206-9
- Author:
- Glasa, Miroslav; Predajňa, Lukáš; Šoltys, Katarína; Sabanadzovic, Sead; Olmos, Antonio
- Source:
- Virus genes 2015 v.51 no.1 pp. 112-121
- ISSN:
- 0920-8569
- Subject:
- Vitis; genome; high-throughput nucleotide sequencing; nucleotide sequences; phylogeny; reverse transcriptase polymerase chain reaction; viruses; Central European region; Czech Republic; Slovakia
- Abstract:
- ... Grapevine Syrah virus-1 (GSyV-1) was identified by small-RNA deep sequencing in Slovak grapevine co-infected by several other viruses. The RT-PCR assays developed in this work substantially improved the virus detection and allowed the identification of GSyV-1 in tested grapevine samples from Slovakia and the Czech Republic at an unexpectedly high rate (ca. 30 %). Subsequently, complete genome sequ ...
- DOI:
- 10.1007/s11262-015-1201-1
-
http://dx.doi.org/10.1007/s11262-015-1201-1
- Author:
- Sano, Kaori; Okazaki, Sachiko; Taniguchi, Satoshi; Masangkay, Joseph S.; Puentespina, Roberto, Jr.; Eres, Eduardo; Cosico, Edison; Quibod, Niña; Kondo, Taisuke; Shimoda, Hiroshi; Hatta, Yuuki; Mitomo, Shumpei; Oba, Mami; Katayama, Yukie; Sassa, Yukiko; Furuya, Tetsuya; Nagai, Makoto; Une, Yumi; Maeda, Ken; Kyuwa, Shigeru; Yoshikawa, Yasuhiro; Akashi, Hiroomi; Omatsu, Tsutomu; Mizutani, Tetsuya
- Source:
- Virus genes 2015 v.51 no.1 pp. 136-139
- ISSN:
- 0920-8569
- Subject:
- Chiroptera; Gammaherpesvirinae; Retroviridae; blood coagulation; genes; high-throughput nucleotide sequencing; hosts; infectious diseases; intestines; monitoring; nucleotide sequences; phylogeny; polymerase chain reaction; viruses; Philippines
- Abstract:
- ... Bats are natural hosts of many zoonotic viruses. Monitoring bat viruses is important to detect novel bat-borne infectious diseases. In this study, next generation sequencing techniques and conventional PCR were used to analyze intestine, lung, and blood clot samples collected from wild bats captured at three locations in Davao region, in the Philippines in 2012. Different viral genes belonging to ...
- DOI:
- 10.1007/s11262-015-1197-6
-
http://dx.doi.org/10.1007/s11262-015-1197-6
- Author:
- Shaozhou, Wulin; Li, Chenxi; Zhang, Qingshan; Meng, Runzhe; Gao, Youlan; Liu, Hongyu; Bai, Xiaofei; Chen, Yuhuan; Liu, Ming; Liu, Siguo; Zhang, Yun
- Source:
- Virus genes 2015 v.51 no.1 pp. 39-44
- ISSN:
- 0920-8569
- Subject:
- Tembusu virus; apoptosis; cultured cells; cytopathogenicity; ducks; fibroblasts; glycoproteins; mammals; proteinases; viruses
- Abstract:
- ... The cytopathic effect produced in cells infected with duck tembusu virus (DTMUV) suggests that this emerging virus may induce apoptosis in primary cultures of duck embryo fibroblasts (DEF). Here, we present evidence that DTMUV infection of cultured cells activates apoptosis and that the ability of DTMUV to induce apoptosis is not restricted to cell type because DTMUV-induced apoptosis in duck and ...
- DOI:
- 10.1007/s11262-015-1200-2
-
http://dx.doi.org/10.1007/s11262-015-1200-2
- Author:
- Sauder, Christian J.; Ngo, Laurie; Simonyan, Vahan; Cong, Yu; Zhang, Cheryl; Link, Malen; Malik, Tahir; Rubin, Steven A.
- Source:
- Virus genes 2015 v.51 no.1 pp. 12-24
- ISSN:
- 0920-8569
- Subject:
- in vivo studies; high-throughput nucleotide sequencing; genome; mutation; virulence; viruses; Mumps orthorubulavirus; DNA-directed RNA polymerase; virus replication
- Abstract:
- ... As a member of the family paramyxoviridae, subfamily paramyxovirinae, the genome of mumps virus (MuV) is postulated to be polyhexameric in length in order to be able to replicate efficiently. While all natural MuV strains sequenced so far obey to this “rule of six,” we describe here the isolation of recombinant MuVs that appeared to contain an additional U residue in the homopolymeric tract of the ...
- DOI:
- 10.1007/s11262-015-1204-y
-
http://dx.doi.org/10.1007/s11262-015-1204-y
- Author:
- Yang, Dequan; Liu, Jian; Ju, Houbin; Ge, Feifei; Wang, Jian; Li, Xin; Zhou, Jinping; Liu, Peihong
- Source:
- Virus genes 2015 v.51 no.1 pp. 25-32
- ISSN:
- 0920-8569
- Subject:
- Influenza A virus; ducks; genes; hemagglutinins; markets; monitoring; pandemic; phylogeny; pigeons; sequence analysis; slaughterhouses; viruses; wild birds; China
- Abstract:
- ... Five H3N2 avian influenza viruses (AIVs) were isolated from live poultry markets (LPMs) and poultry slaughterhouses in Shanghai, China in 2013. All viruses were characterized by whole-genome sequencing with subsequent genetic comparison and phylogenetic analysis. The hemagglutinin cleavage site of all viruses indicated that the five strains were low-pathogenic AIVs. Phylogenetic analysis of all ei ...
- DOI:
- 10.1007/s11262-015-1198-5
-
http://dx.doi.org/10.1007/s11262-015-1198-5
- Author:
- Hiono, Takahiro; Ohkawara, Ayako; Ogasawara, Kohei; Okamatsu, Masatoshi; Tamura, Tomokazu; Chu, Duc-Huy; Suzuki, Mizuho; Kuribayashi, Saya; Shichinohe, Shintaro; Takada, Ayato; Ogawa, Hirohito; Yoshida, Reiko; Miyamoto, Hiroko; Nao, Naganori; Furuyama, Wakako; Maruyama, Junki; Eguchi, Nao; Ulziibat, Gerelmaa; Enkhbold, Bazarragchaa; Shatar, Munkhduuren; Jargalsaikhan, Tserenjav; Byambadorj, Selenge; Damdinjav, Batchuluun; Sakoda, Yoshihiro; Kida, Hiroshi
- Source:
- Virus genes 2015 v.51 no.1 pp. 57-68
- ISSN:
- 0920-8569
- Subject:
- Influenza A virus; autumn; avian influenza; feces; humans; monitoring; nucleotide sequences; phylogeny; poultry; viruses; water birds; wild birds; China; Japan; Mongolia; Siberia
- Abstract:
- ... Migratory water birds are the natural reservoir of influenza A viruses. H5 and H7 influenza viruses are isolated over the world and also circulate among poultry in Asia. In 2010, two H5N1 highly pathogenic avian influenza viruses (HPAIVs) were isolated from fecal samples of water birds on the flyway of migration from Siberia, Russia to the south in Hokkaido, Japan. H7N9 viruses are sporadically is ...
- DOI:
- 10.1007/s11262-015-1214-9
-
http://dx.doi.org/10.1007/s11262-015-1214-9
- Author:
- da Silva, Flavio R. C.; Daudt, Cíntia; Streck, André F.; Weber, Matheus N.; Filho, Ronaldo V. Leite; Driemeier, David; Canal, Cláudio W.
- Source:
- Virus genes 2015 v.51 no.1 pp. 77-84
- ISSN:
- 0920-8569
- Subject:
- Papillomaviridae; infectious diseases; papilloma; open reading frames; cattle; cross immunity; viruses; genotype; vaccination; genetic variation; histopathology; Brazil; Amazonia
- Abstract:
- ... Papillomaviruses are small and complex viruses that belong to the Papillomaviridae family, which comprises 39 genera. The bovine papillomavirus (BPV) causes an infectious disease that is characterized by chronic and proliferative benign tumors that affect cattle worldwide. Different genotypes of BPVs can cause distinct skin and mucosal lesions and the immunity they raise has low cross-protection. ...
- DOI:
- 10.1007/s11262-015-1220-y
-
http://dx.doi.org/10.1007/s11262-015-1220-y
- Author:
- Zhong, Jie; Chen, Chuan-Yuan; Gao, Bi-Da
- Source:
- Virus genes 2015 v.51 no.1 pp. 167-170
- ISSN:
- 0920-8569
- Subject:
- Thanatephorus cucumeris; databases; double-stranded RNA; dsRNA viruses; genome; molecular weight; mycoviruses; nucleotides; open reading frames; plant pathogenic fungi; sequence analysis
- Abstract:
- ... Here we present the genome sequence of a novel dsRNA virus we designed as Rhizoctonia solani RNA virus HN008 (RsRV-HN008) from a filamentous fungus R. solani. Its genome (7596 nucleotides) contains two non-overlapping open reading frames (ORF1 and ORF2). ORF1 encoded a 128 kDa protein that showed no significant identity to any other virus sequence in the NCBI database. ORF2 encoded a protein with ...
- DOI:
- 10.1007/s11262-015-1219-4
-
http://dx.doi.org/10.1007/s11262-015-1219-4
- Author:
- Mahony, Timothy J.; Hall, Robyn N.; Walkden-Brown, Stephen; Meers, Joanne; Gravel, Jennifer L.; West, Lani; Hardy, Vanessa; Islam, A.F.M. Fakhrul; Fowler, Elizabeth V.; Mitter, Neena
- Source:
- Virus genes 2015 v.51 no.1 pp. 85-95
- ISSN:
- 0920-8569
- Subject:
- Meleagrid alphaherpesvirus 1; hosts; turkeys; clones; viruses; genes; Gallid alphaherpesvirus 2; Marek disease; protein-serine-threonine kinases; single nucleotide polymorphism
- Abstract:
- ... Meleagrid herpesvirus 1 (MeHV-1 or turkey herpesvirus) has been widely used as a vaccine in commercial poultry. Initially, these vaccine applications were for the prevention of Marek’s disease resulting from Gallid herpesvirus 2 infections, while more recently MeHV-1 has been used as recombinant vector for other poultry infections. The construction of herpesvirus infectious clones that permit prop ...
- DOI:
- 10.1007/s11262-015-1216-7
-
http://dx.doi.org/10.1007/s11262-015-1216-7
- Author:
- Hause, Ben M.; Hesse, Richard A.; Anderson, Gary A.
- Source:
- Virus genes 2015 v.51 no.1 pp. 144-147
- ISSN:
- 0920-8569
- Subject:
- Mycoplasma; Picornavirales; RNA-directed RNA polymerase; Ureaplasma; amino acids; coat proteins; databases; feces; metagenomics; open reading frames; phylogeny; proteinases; swine; virulence; viruses
- Abstract:
- ... De novo assembly of metagenomic sequencing reads from feces from a clinically normal pig identified two approximately 9 kb contigs which each consisted of a single large open reading frame. While one contig encoded a predicted 2990 amino acid protein with 83 % identity to the recently described posavirus 1, the other contig encoded a predicted 2942 amino acid protein with only 25 % identity limite ...
- DOI:
- 10.1007/s11262-015-1215-8
- CHORUS:
- 10.1007/s11262-015-1215-8
-
http://dx.doi.org/10.1007/s11262-015-1215-8
- Author:
- Goraya, Mohsan Ullah; Qureshi, Zafar ul Ahsan; Abbas, Muhammad; Ashraf, Muhammad; Munir, Muhammad
- Source:
- Virus genes 2015 v.51 no.1 pp. 45-50
- ISSN:
- 0920-8569
- Subject:
- Poxviridae; amino acids; buffaloes; genes; human diseases; humans; mastitis; mutation; phylogeny; sequence analysis; teats; viruses; Pakistan
- Abstract:
- ... Outbreaks of buffalopox affect udder and teats, which may ultimately lead to mastitis in dairy buffalo and can significantly compromise the production. In this study, we report isolation of buffalo poxvirus and sequence analysis of the B5R gene collected from the buffalo clinically suspected to be poxvirus infected. The virus was isolated on BHK-21 cell line and was passaged for 50 times, B5R gene ...
- DOI:
- 10.1007/s11262-015-1209-6
-
http://dx.doi.org/10.1007/s11262-015-1209-6
- Author:
- Sato, Go; Kawashima, Taketo; Kiuchi, Masahiro; Tohya, Yukinobu
- Source:
- Virus genes 2015 v.51 no.1 pp. 148-151
- ISSN:
- 0920-8569
- Subject:
- Callosciurus; metagenomics; new species; phylogeny; squirrels; Japan; Taiwan
- Abstract:
- ... A novel cyclovirus was identified in the intestinal contents of Taiwan squirrels (Callosciurus erythraeus thaiwanensis) collected in Kanagawa prefecture, Japan, by metagenomic analysis, and was named Taiwan squirrel cyclovirus-1 (TsCyV-1). Phylogenetic analysis showed that TsCyV-1 formed a branch separate from other representative cyclovirus strains. TsCyV-1 is considered to be a new species in th ...
- DOI:
- 10.1007/s11262-015-1217-6
-
http://dx.doi.org/10.1007/s11262-015-1217-6