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1. Characterization of a replication-competent vector encoding DsRed based on a human adenovirus type 4 a-like strain

Author:
Tian, Xingui; Chen, Yong; Li, Haitao; Fan, Ye; Zhou, Zhichao; Chen, Shiying; Li, Xiao; Zhou, Rong
Source:
Virus research 2019 v.270 pp. 197662
ISSN:
0168-1702
Subject:
Mastadenovirus; adults; antibody formation; children; etiological agents; gene therapy; genome; genomics; hamsters; mice; neutralizing antibodies; nucleotide sequences; patients; prototypes; rapid methods; respiratory tract diseases; screening; vaccines; viruses; China
Abstract:
... Human adenovirus type 4 (HAdV4) is an etiological agent of acute respiratory disease (ARD) in pediatric and adult patients. HAdV4 strains can be divided into two major genomic clusters, namely prototype (p)-like viruses and a-like viruses. Here, the complete genome sequence of HAdV4 strain GZ01, isolated from a child with ARD in southern China, is first reported and analyzed. This strain was deter ...
DOI:
10.1016/j.virusres.2019.197662

2. Characterization of three novel betapartitiviruses co-infecting the phytopathogenic fungus Rhizoctonia solani

Author:
Chen, Yi; Tong Gai, Xiao; Xing Chen, Ruo; Li, Chang Xin; Zhao, Gao Kun; Yuan Xia, Zhen; Zou, Cong Ming; Zhong, Jie
Source:
Virus research 2019 v.270 pp. 197649
ISSN:
0168-1702
Subject:
RNA-directed RNA polymerase; Rhizoctonia solani virus 717; Thanatephorus cucumeris; coat proteins; databases; double-stranded RNA; genome; hyphae; mixed infection; mycoviruses; phenotype; phylogeny; plant pathogenic fungi; sequence homology; taxonomy
Abstract:
... In this study, we isolated and characterized seven dsRNA elements, designed as dsRNA-1 to 7, from a Rhizoctonia solani strain. Sequence analysis indicated that there were at least three novel mycoviruses co-infected in this fungal strain, termed Rhizoctonia solani partitivirus 6 (RsPV6), Rhizoctonia solani partitivirus 7 (RsPV7), and Rhizoctonia solani partitivirus 8 (RsPV8), respectively. RsPV6 c ...
DOI:
10.1016/j.virusres.2019.197649

3. Identification of a functional nuclear localization signal in 3Dpol/3CD of duck hepatitis A virus 1

Author:
Chen, Jun-Hao; Zhang, Rui-Hua; Lin, Shao-Li; Li, Peng-Fei; Lan, Jing-Jing; Gao, Ji-Ming; Xie, Zhi-Jing; Li, Fu-Chang; Jiang, Shi-Jin
Source:
Virus research 2019 v.270 pp. 197670
ISSN:
0168-1702
Subject:
Duck hepatitis A virus; genome; nuclear localization signals; poultry diseases; structural proteins
Abstract:
... The nuclear localization signals (NLS) were usually composed of basic residues (K and R) and played an important role in delivery of genomes and structural protein into nucleus. In this research, we identified that 3Dpol/3CD entered into nucleus during viral propagation of duck hepatitis A virus type 1 (DHAV-1). To investigate the reason that 3Dpol/3CD entered into nucleus, the amino acid sequence ...
DOI:
10.1016/j.virusres.2019.197670

4. Successful establishment of a reverse genetic system for QX-type infectious bronchitis virus and technical improvement of the rescue procedure

Author:
Zhao, Ye; Cheng, Jinlong; Xu, Gang; Thiel, Volker; Zhang, Guozhong
Source:
Virus research 2019
ISSN:
0168-1702
Subject:
Infectious bronchitis virus; Vaccinia virus; cell culture; chicken eggs; complementary DNA; culture media; genome; genomics; homologous recombination; messenger RNA; molecular biology; mutation; pathogenicity; reverse genetics; specific pathogen-free animals; transcription (genetics); transfection; viruses
Abstract:
... In this study, a pathogenic avian infectious bronchitis virus (IBV) QX-type strain YN was successfully rescued by vaccinia virus based reverse genetic technology. Ten fragments contiguously spanning the complete IBV genome were amplified and cloned into the vaccinia virus genome by homologous recombination. The full-length genomic cDNA was transcribed in vitro, and its transcript was transfected i ...
DOI:
10.1016/j.virusres.2019.197726

5. Mitoviruses in the conifer root rot pathogens Heterobasidion annosum and H. parviporum

Author:
Vainio, Eeva J.
Source:
Virus research 2019
ISSN:
0168-1702
Subject:
Heterobasidion annosum; Mitovirus; conifers; data collection; fungi; genome; hosts; pathogens; reverse transcriptase polymerase chain reaction; root rot; sequence analysis; viruses
Abstract:
... Mitoviral infections are highly common among fungi, but so far only one mitovirus has been described in Heterobasidion spp. conifer pathogens. Here, the occurrence of further mitoviruses was investigated using a previously published RNA-Seq dataset for de novo contig assembly. This allowed the identification of two additional mitovirus strains designated as Heterobasidion mitovirus 2 (HetMV2) and ...
DOI:
10.1016/j.virusres.2019.197681

6. First identification of Sapoviruses in wild boar

Author:
Katsuta, Risako; Sunaga, Fujiko; Oi, Toru; Doan, Yen Hai; Tsuzuku, Satoko; Suzuki, Yoshihisa; Sano, Kaori; Katayama, Yukie; Omatsu, Tsutomu; Oba, Mami; Furuya, Tetsuya; Ouchi, Yoshinao; Shirai, Junsuke; Mizutani, Tetsuya; Oka, Tomoichiro; Nagai, Makoto
Source:
Virus research 2019
ISSN:
0168-1702
Subject:
complementary DNA; feces; genome; genotype; humans; metagenomics; phylogeny; rapid amplification of cDNA ends; reverse transcriptase polymerase chain reaction; swine; viruses; wild boars; Japan
Abstract:
... Sapoviruses (SaVs) are enteric viruses that have been detected in human and animals previously; however, SaVs have not been identified in wild boar yet. Using a metagenomics approach, we identified SaVs in fecal samples of free-living wild boars in Japan for the first time. Six of the 48 specimens identified belonged to one genogroup (G)III, one GV and four GVI SaV sequence reads. We successfully ...
DOI:
10.1016/j.virusres.2019.197680

7. Transcriptional activation of long terminal repeat of bovine leukemia virus by bovine heat shock factor 1

Author:
Hachiya, Yuma; Oguma, Keisuke; Koshida, Takeo; Koba, Ryota; Sentsui, Hiroshi
Source:
Virus research 2019 v.269 pp. 197641
ISSN:
0168-1702
Subject:
Bovine leukemia virus; DNA-binding domains; cats; cattle; enzootic bovine leukosis; gene expression; genome; heat shock proteins; heat shock response; heat stress; luciferase; mutants; terminal repeat sequences; transactivators; transcription (genetics); transcriptional activation
Abstract:
... Bovine leukemia virus (BLV) causes enzootic bovine leukosis (EBL). The BLV genome encodes Tax protein, a transcriptional activator of viral gene expression that binds to the BLV long terminal repeat (LTR). Heat shock factor 1 (HSF1) is a known regulator of the heat shock response proteins, including heat shock proteins. In the present study, the BLV LTR was investigated for interaction of heat sho ...
DOI:
10.1016/j.virusres.2019.197641

8. Serine Protease Inhibitor AEBSF Reduces Dengue Virus Infection via Decreased Cholesterol Synthesis

Author:
Sreelatha, Liji; Malakar, Shilu; Songprakhon, Pucharee; Morchang, Atthapan; Srisawat, Chatchawan; Noisakran, Sansanee; Yenchitosomanus, Pa-thai; Limjindaporn, Thawornchai
Source:
Virus research 2019
ISSN:
0168-1702
Subject:
Dengue virus; antiviral agents; antiviral properties; aromatic compounds; cell lines; cholesterol; dengue hemorrhagic fever; dose response; enzyme activity; gene expression; genome; geographical distribution; human diseases; low density lipoprotein; low density lipoprotein receptors; patients; protein synthesis; proteinase inhibitors; serine proteinases; serotypes; viral load; virus replication
Abstract:
... Dengue virus (DENV) infection has evolved into a major global health menace and economic burden due to its intensity and geographic distribution. DENV infection in humans can cause a wide range of symptoms including dengue fever (DF), dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). An antiviral agent that is effective against all four serotypes of DENV is urgently needed to prevent ...
DOI:
10.1016/j.virusres.2019.197672

9. Complete genome sequence and characterization of a new iflavirus from the small brown planthopper (Laodelphax striatellus)

Author:
Wu, Nan; Zhang, Peipei; Liu, Wenwen; Cao, Mengji; Massart, Sebastien; Wang, Xifeng
Source:
Virus research 2019
ISSN:
0168-1702
Subject:
Iflavirus; Laodelphax striatellus; Nilaparvata lugens; RNA-directed RNA polymerase; amino acid sequences; females; genome; honeydew; messenger RNA; new species; nucleotides; open reading frames; phylogeny; polyproteins; proteinases; reproduction; sequence analysis; structural proteins
Abstract:
... A novel iflavirus, tentatively named laodelphax striatellus iflavirus 1 (LsIV1), was identified in Laodelphax striatellus by total RNA-sequencing, and its genome sequence was confirmed by Sanger sequencing. The complete genome consisted of 10,831 nucleotides with a polyA tail and included one open reading frame, encoding a 361.7-kD polyprotein. Conserved motifs for structural proteins, helicase, p ...
DOI:
10.1016/j.virusres.2019.197651

10. The effect of genetic complementation on the fitness and diversity of viruses spreading as collective infectious units

Author:
Segredo-Otero, Ernesto; Sanjuán, Rafael
Source:
Virus research 2019 v.267 pp. 41-48
ISSN:
0168-1702
Subject:
adverse effects; bacteria; capsid; dendritic cells; genetic complementation; genetic relationships; genetic variation; genome; mutation accumulation; polyploidy; risk; virion; viruses
Abstract:
... Viruses can spread collectively using different types of structures such as extracellular vesicles, virion aggregates, polyploid capsids, occlusion bodies, and even cells that accumulate virions at their surface, such as bacteria and dendritic cells. Despite the mounting evidence for collective spread, its implications for viral fitness and diversity remain poorly understood. It has been postulate ...
DOI:
10.1016/j.virusres.2019.05.005

11. Characterization and genome analysis of the temperate bacteriophage φSAJS1 from Streptomyces avermitilis

Author:
Lu, Nana; Kim, Choljin; Chen, Zhi; Wen, Ying; Wei, Qing; Qiu, Yi; Wang, Shiwei; Song, Yuan
Source:
Virus research 2019 v.265 pp. 34-42
ISSN:
0168-1702
Subject:
DNA; DNA packaging; Siphoviridae; Streptomyces avermitilis; Streptomyces griseus; antibiotics; bacteria; bacteriophages; calcium; evolution; fermentation; genome; growth curves; host range; open reading frames; pH; proteins; sequence analysis; temperature; transmission electron microscopy
Abstract:
... Streptomyces is an important antibiotic-producing bacterium; however, antibiotic production is often negatively affected by bacteriophage contamination. In the present study, the temperate phage φSAJS1 was isolated and characterized from an unsuccessful Streptomyces avermitilis fermentation culture. The complete genome of phage φSAJS1 was sequenced. Phage φSAJS1 belongs to the Siphoviridae family ...
DOI:
10.1016/j.virusres.2019.03.006

12. Extracellular vesicles: Vehicles of en bloc viral transmission

Author:
Altan-Bonnet, Nihal; Perales, Celia; Domingo, Esteban
Source:
Virus research 2019 v.265 pp. 143-149
ISSN:
0168-1702
Subject:
DNA viruses; RNA; RNA viruses; genome; hosts; mutagenesis; mutants; virion; virulence; virus transmission; viruses
Abstract:
... En Bloc transmission of viruses allow multiple genomes to collectivelly penetrate and initiate infection in the same cell, often resulting in enhanced infectivity. Given the quasispecies (mutant cloud) nature of RNA viruses and many DNA viruses, en bloc transmission of multiple genomes provides different starting points in sequence space to initiate adaptive walks, and has implications for modulat ...
DOI:
10.1016/j.virusres.2019.03.023

13. Strength in numbers: Mechanisms of viral co-infection

Author:
Aguilera, Elizabeth R.; Pfeiffer, Julie K.
Source:
Virus research 2019 v.265 pp. 43-46
ISSN:
0168-1702
Subject:
RNA viruses; RNA-directed RNA polymerase; bacteria; genome; mixed infection; virus transmission; viruses
Abstract:
... RNA virus populations are diverse due to a variety of factors, including lack of proofreading of the viral RNA-dependent RNA polymerase. These diverse viral populations include defective viruses incapable of productive infection. Recent studies have determined the existence of several modes of viral transmission outside of canonical pathways, including en bloc transmission of multiple viruses into ...
DOI:
10.1016/j.virusres.2019.03.003

14. Stronger together: Multi-genome transmission of measles virus

Author:
Cattaneo, Roberto; Donohue, Ryan C.; Generous, Alex R.; Navaratnarajah, Chanakha K.; Pfaller, Christian K.
Source:
Virus research 2019 v.265 pp. 74-79
ISSN:
0168-1702
Subject:
proteins; lymphatic system; humans; immunosuppression; genome; epithelial cells; hosts; Measles morbillivirus; children; viruses; functional properties; genetic variation; epithelium; tissue culture
Abstract:
... Measles virus (MeV) is an immunosuppressive, extremely contagious RNA virus that remains a leading cause of death among children. MeV is dual-tropic: it replicates first in lymphatic tissue, causing immunosuppression, and then in epithelial cells of the upper airways, accounting for extremely efficient contagion. Efficient contagion is counter-intuitive because the enveloped MeV particles are larg ...
DOI:
10.1016/j.virusres.2019.03.007

15. The evolution of collective infectious units in viruses

Author:
Leeks, Asher; Sanjuán, Rafael; West, Stuart A.
Source:
Virus research 2019 v.265 pp. 94-101
ISSN:
0168-1702
Subject:
evolution; genome; hosts; models; packaging; pathogenesis; virion; virus replication; viruses
Abstract:
... Viruses frequently spread among cells or hosts in groups, with multiple viral genomes inside the same infectious unit. These collective infectious units can consist of multiple viral genomes inside the same virion, or multiple virions inside a larger structure such as a vesicle. Collective infectious units deliver multiple viral genomes to the same cell simultaneously, which can have important imp ...
DOI:
10.1016/j.virusres.2019.03.013

16. To assemble or not to assemble: The changing rules of pneumovirus transmission

Author:
Cifuentes-Muñoz, Nicolás; Ellis Dutch, Rebecca
Source:
Virus research 2019 v.265 pp. 68-73
ISSN:
0168-1702
Subject:
Human metapneumovirus; Pneumovirus; RNA; Respiratory syncytial virus; genome; genomics; giant cells; immune response; inclusion bodies; microfilaments; nucleocapsid; organelles; pathogens; public health; risk; virion; virus transmission; viruses
Abstract:
... Pneumoviruses represent a major public health burden across the world. Respiratory syncytial virus (RSV) and human metapneumovirus (HMPV), two of the most recognizable pediatric infectious agents, belong to this family. These viruses are enveloped with a non-segmented negative-sense RNA genome, and their replication occurs in specialized cytosolic organelles named inclusion bodies (IB). The critic ...
DOI:
10.1016/j.virusres.2019.03.002

17. Early events in rabies virus infection—Attachment, entry, and intracellular trafficking

Author:
Guo, Yidi; Duan, Ming; Wang, Xinping; Gao, Jie; Guan, Zhenhong; Zhang, Maolin
Source:
Virus research 2019 v.263 pp. 217-225
ISSN:
0168-1702
Subject:
death; Rabies lyssavirus; public health; virion; rabies; capsid; genome; physiological transport; ribonucleoproteins; models; endocytosis; viruses; gangliosides; actin; receptors; RNA; virus replication
Abstract:
... Rabies virus (RABV), an enveloped virus with a single-stranded and negative-sense RNA genome, is the type species of the Lyssavirus Genus within the Rhabdoviridae family. As the causative agent of rabies with a nearly 100% fatality, the neurotropic RABV pose a serious threat to the global public health. Though a great effort has been made toward understanding the molecular mechanism underlying vir ...
DOI:
10.1016/j.virusres.2019.02.006

18. Enhanced bioinformatic profiling of VIDISCA libraries for virus detection and discovery

Author:
Kinsella, Cormac M.; Deijs, Martin; van der Hoek, Lia
Source:
Virus research 2019 v.263 pp. 21-26
ISSN:
0168-1702
Subject:
Human immunodeficiency virus 1; Pestivirus; adults; bioinformatics; blood serum; computer software; databases; feces; genome; high-throughput nucleotide sequencing; humans; metagenomics; molecular biology; nucleotide sequences; parasites; restriction endonucleases; ribosomal RNA; swine; viruses
Abstract:
... VIDISCA is a next-generation sequencing (NGS) library preparation method designed to enrich viral nucleic acids from samples before highly-multiplexed low depth sequencing. Reliable detection of known viruses and discovery of novel divergent viruses from NGS data require dedicated analysis tools that are both sensitive and accurate. Existing software was utilised to design a new bioinformatic work ...
DOI:
10.1016/j.virusres.2018.12.010

19. Molecular and biological characterization of a novel botybirnavirus identified from a Pakistani isolate of Alternaria alternata

Author:
Shamsi, Wajeeha; Sato, Yukiyo; Jamal, Atif; Shahi, Sabitree; Kondo, Hideki; Suzuki, Nobuhiro; Bhatti, Muhammad Faraz
Source:
Virus research 2019 v.263 pp. 119-128
ISSN:
0168-1702
Subject:
Alternaria alternata; Botryotinia porri; Chrysoviridae; RNA-directed RNA polymerase; Totiviridae; amino acids; double-stranded RNA; electron microscopy; fungi; genome; hosts; mycoviruses; phylogeny; protoplast fusion; structural proteins
Abstract:
... Mycoviruses ubiquitously infect a wide range of fungal hosts in the world. The current study reports a novel double stranded RNA (dsRNA) virus, termed Alternaria alternata botybirnavirus 1 (AaBbV1), infecting a Pakistani strain, 4a, of a phytopathogenic ascomycetous fungus Alternaria alternata. A combined approach of next generation and conventional terminal end sequencing of the viral genome reve ...
DOI:
10.1016/j.virusres.2019.01.006

20. Molecular characterisation of a novel gemycircularvirus associated with olive trees in Italy

Author:
Chiumenti, Michela; Greco, Claudia; Antelmi, Ilaria; Sion, Valentina; Altamura, Giuseppe; Nigro, Franco; Saldarelli, Pasquale
Source:
Virus research 2019 v.263 pp. 169-172
ISSN:
0168-1702
Subject:
Olea europaea; branches; genome; leaves; olives; open reading frames; proteins; sequence analysis; single-stranded DNA; surveys; tissues; viruses; Italy
Abstract:
... A new gemycircularvirus sequence was obtained from total DNAs extracted from olive leaves and twigs tissues. Its complete genome consists of a single-stranded circular DNA of 2,145 nt, whose sequence was obtained by rolling circle amplification (RCA). Genome analysis identified three open reading frames, sharing homologies with the coat and replication-associated proteins, these latter in the anti ...
DOI:
10.1016/j.virusres.2019.01.016