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You searched for: Journal Zeitschrift für Naturforschung Remove constraint Journal: Zeitschrift für Naturforschung Publication Year 2014 Remove constraint Publication Year: 2014 Source 2014 v.65 no.3-4 Remove constraint Source: 2014 v.65 no.3-4
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1. Amylolytic Lactobacillus Strains from Bulgarian Fermented Beverage Boza

Author:
Petrova, Penka; Emanuilova, Milena; Petrov, Kaloyan
Source:
Zeitschrift für Naturforschung 2014 v.65 no.3-4 pp. 218-224
ISSN:
0939-5075
Subject:
Lactobacillus pentosus; Lactobacillus plantarum; amylases; beverages; carbon; enzyme activity; fermented foods; hydrolysis; lactic acid; lactic acid bacteria; lactic fermentation; meat extracts; pH; ribosomal DNA; starch; succinic acid; yeast extract; yeasts
Abstract:
... The lactic acid fermentation is a worldwide method for cereal processing. Great diversity of fermented foods and drinks is produced with the participation of amylolytic lactic acid bacteria (ALAB). In the present study the ALAB content of the Bulgarian cereal beverage “boza” was investigated. Two strains, Bom 816 and N3, were found to possess significant amylolytic activity. The strains’ identific ...
DOI:
10.1515/znc-2010-3-409

2. Assessment of Enzyme Inhibitory and Antioxidant Activities of Lignans from Taxus baccata L.

Author:
Kucukboyacı, Nurgun; Orhan, Ilkay; Şener, Bilge; Nawaz, S. Ahmad; Choudhary, M. Iqbal
Source:
Zeitschrift für Naturforschung 2014 v.65 no.3-4 pp. 187-194
ISSN:
1865-7125
Subject:
Alzheimer disease; acetylcholinesterase; ions; Taxus baccata; 2,2-diphenyl-1-picrylhydrazyl; iron; linoleate 13S-lipoxygenase; chromatography; pathogenesis; lignans; cholinesterase; chlorogenic acid; antioxidant activity
Abstract:
... Phytochemical investigations of Taxus baccata L. by successive chromatographic methods resulted in the isolation of the lignans lariciresinol (1), taxiresinol (2), 3´-demethylisolariciresinol- 9´-hydroxyisopropylether (3), isolariciresinol (4), and 3-demethylisolariciresinol (5) as well as taxoids. Compounds 1 - 5 were evaluated for their acetylcholinesterase (AChE), butyrylcholinesterase (BChE), ...
DOI:
10.1515/znc-2010-3-404

3. Current Results on Biological Activities of Lichen Secondary Metabolites: a Review

Author:
Molnár, Katalin; Farkas, Edit
Source:
Zeitschrift für Naturforschung 2014 v.65 no.3-4 pp. 157-173
ISSN:
1865-7125
Subject:
Cyanobacteria; algae; analytical methods; antioxidant activity; chemotaxonomy; fungi; homeostasis; lichens; photostability; pollution; secondary metabolites; symbiosis; thallus
Abstract:
... Lichens are symbiotic organisms of fungi and algae or cyanobacteria. Lichen-forming fungi synthesize a great variety of secondary metabolites, many of which are unique. Developments in analytical techniques and experimental methods have resulted in the identification of about 1050 lichen substances (including those found in cultures). In addition to their role in lichen chemotaxonomy and systemati ...
DOI:
10.1515/znc-2010-3-401

4. Differential Expression of Thaumatin-Like Proteins in Sorghum Infested with Greenbugs

Author:
Chou, Juan; Huang, Yinghua
Source:
Zeitschrift für Naturforschung 2014 v.65 no.3-4 pp. 271-276
ISSN:
1865-7125
Subject:
Schizaphis graminum; Sorghum (Poaceae); actin; agarose; complementary DNA; gels; gene expression; gene expression regulation; genes; insects; messenger RNA; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; transcription (genetics)
Abstract:
... This study was designed to quantitatively analyze the expression of thaumatin-like protein (TLP) at the transcriptional level in different sorghum lines when they were infested with greenbugs. Three sorghum lines, Tx7000, PI550607, and PI550610, were used. RNAs were isolated from the different sorghum lines that were infested with greenbugs at different infestation times. The resultant mRNA was re ...
DOI:
10.1515/znc-2010-3-416

5. Essential Region for 3-N Methylation in N-Methyltransferases Involved in Caffeine Biosynthesis

Author:
Mizuno, Kouichi; Kurosawa, Shin-ichi; Yoshizawa, Yuko; Kato, Misako
Source:
Zeitschrift für Naturforschung 2014 v.65 no.3-4 pp. 257-265
ISSN:
1865-7125
Subject:
amino acid sequences; biochemical pathways; biosynthesis; caffeine; histidine; methylation; mutants; substrate specificity; theobromine
Abstract:
... The caffeine biosynthetic pathway is composed of three methylation steps, and N-methyltransferase catalyzing each step has high substrate specificity. Since the amino acid sequences among coffee 7-methylxanthosine synthase (CmXRS1), theobromine synthase, and caffeine synthase are highly homologous to each other, these substrate specificities seem to be determined in a very restricted region. The a ...
DOI:
10.1515/znc-2010-3-414

6. Expression for Caffeine Biosynthesis and Related Enzymes in Camellia sinensis

Author:
Kato, Misako; Kitao, Naoko; Ishida, Mariko; Morimoto, Hanayo; Irino, Fumi; Mizuno, Kouichi
Source:
Zeitschrift für Naturforschung 2014 v.65 no.3-4 pp. 245-256
ISSN:
1865-7125
Subject:
Camellia sinensis; Northern blotting; S-adenosylmethionine; biochemical pathways; biosynthesis; caffeine; dehydrins; flowers; gene expression; genes; heavy metals; leaf development; leaves; methionine adenosyltransferase; naringenin-chalcone synthase; nitrogen; phenylalanine ammonia-lyase; plant hormones; tea; water stress
Abstract:
... Caffeine (1,3,7-trimethylxanthine) is a purine alkaloid that is present in high concentrations in the tea plant Camellia sinensis. Caffeine synthase (CS, EC 2.1.1.160) catalyzes the S-adenosyl-L-methionine-dependent N-3- and N-1-methylation of the purine base to form caffeine, the last step in the purine alkaloid biosynthetic pathway. We studied the expression profile of the tea caffeine synthase ...
DOI:
10.1515/znc-2010-3-413

7. Genotoxic Effect of Epirubicin in Mouse Bone Marrow in vivo

Author:
Sen, Asuman K.; Karakas, Emin; Bilaloglu, Rahmi
Source:
Zeitschrift für Naturforschung 2014 v.65 no.3-4 pp. 211-217
ISSN:
1865-7125
Subject:
antibiotics; body weight; bone marrow; bone marrow cells; cytotoxicity; epirubicin; erythrocytes; mice; mutagens
Abstract:
... The genotoxic effect of epirubicin, a semisynthetic anthracycline antibiotic which has been used as an anticancer drug, was investigated in vivo on bone marrow cells of Swiss albino mice using the micronucleus test. To determine the incidence of micronuclei, mice were injected intraperitoneally with the drug at single doses of 4, 6, 8, and 10 mg/kg body weight. Then, bone marrow was sampled 18, 24 ...
DOI:
10.1515/znc-2010-3-408

8. Immune Responses in Mice after Immunization with Antigens from Different Stages of the Parasite Schistosoma mansoni

Author:
Gaber, Hanaa M.; Maghraby, Amany S.; Ahmed, Mohamed Bastawy; Ruppel, Andreas; Bahgat, Mahmoud M.
Source:
Zeitschrift für Naturforschung 2014 v.65 no.3-4 pp. 289-302
ISSN:
1865-7125
Subject:
B-lymphocytes; Schistosoma mansoni; T-lymphocytes; antigens; eggs; immune response; immunization; immunoglobulin A; immunoglobulin G; immunoglobulin M; lymph nodes; mice; parasites; peptides; splenocytes; thymocytes
Abstract:
... Mice responses to immunization with Schistosoma mansoni antigens were investigated. Priming with cercarial antigen preparation (CAP) induced significant (P < 0.05) IgM, IgG, IgG2a, IgG2b, and IgA increases, while booster caused a significant IgG1 increase. A soluble worm antigen preparation (SWAP) caused significant IgG elevation. Priming with soluble egg antigen (SEA) caused significant IgM and I ...
DOI:
10.1515/znc-2010-3-419

9. In vitro Antioxidant and Antiproliferative Activities of Flavonoids from Ailanthus excelsa (Roxb.) (Simaroubaceae) Leaves

Author:
Said, Ataa; Tundis, Rosa; Hawas, Usama W.; El-Kousy, Salah M.; Rashed, Khaled; Menichini, Federica; Bonesi, Marco; Huefner, Antje; Loizzo, Monica Rosa
Source:
Zeitschrift für Naturforschung 2014 v.65 no.3-4 pp. 180-186
ISSN:
1865-7125
Subject:
2,2-diphenyl-1-picrylhydrazyl; Ailanthus excelsa; antioxidant activity; antioxidants; apigenin; cell growth; chemical composition; chromatography; humans; inhibitory concentration 50; kaempferol; leaves; luteolin; melanoma; methanol; quercetin; traditional medicine
Abstract:
... The present study aimed to investigate the chemical composition, and the antioxidant and antiproliferative activities of Ailanthus excelsa, a plant used in Egyptian traditional medicine. Chromatographic separation of a methanol extract of A. excelsa leaves yielded four fl avones, namely apigenin (1), apigenin 7-O-β-glucoside (2), luteolin (3), and luteolin 7-O-β-glucoside (4), and seven fl avonols ...
DOI:
10.1515/znc-2010-3-403

10. In vitro Effects of Chlorpyrifos on the Acetylcholinesterase Activity of Euryhaline Fish, Oreochromis mossambicus

Author:
Venkateswara Rao, Janapala; Kavitha, Pathakoti
Source:
Zeitschrift für Naturforschung 2014 v.65 no.3-4 pp. 303-306
ISSN:
0939-5075
Subject:
Oreochromis mossambicus; acetylcholinesterase; brain; chlorpyrifos; diagnostic techniques; environmental monitoring; euryhaline species; neurotoxicity
Abstract:
... The in vitro effect of a widely used organophosphorus insecticide, chlorpyrifos (CPP), on the acetylcholinesterase (AChE) activity was studied in vitro. The kinetic constants Kₘ and Vₘₐₓ and the bimolecular constant ki were determined in vitro. The in vitro AChE study indicated that CPP is neurotoxic and that it alters the apparent Kₘ values widely in a concentration-dependent manner, resulting in ...
DOI:
10.1515/znc-2010-3-420

11. Isolation and Identification of Poplar Isoplastocyanins

Author:
Dimitrov, Mitko I.; Donchev, Anthony A.; Shosheva, Alexandra C.; Getov, Vladimir I.; Terezova, Nedyalka P.; Stoichev, Svetozar D.
Source:
Zeitschrift für Naturforschung 2014 v.65 no.3-4 pp. 225-230
ISSN:
1865-7125
Subject:
Populus; absorbance; chromatography; dimorphism; isoelectric focusing; isoelectric point; pH; photosynthesis; plastocyanin; spectroscopy
Abstract:
... An improved four-stage isolation and purification procedure for preparing poplar isoplastocyanins is described in detail. Absorbance (UV-VIS) spectroscopy and isoelectric focusing (IEF) are used to determine the protein purity and identity. The present procedure increases twice the total plastocyanin (PC) yield. Four PC isoform fractions are consecutively isolated at the third chromatographic step ...
DOI:
10.1515/znc-2010-3-410

12. Isolation of Quality Total RNA from the Aromatic Plant Origanum onites

Author:
Poyraz, Īsmail; Sözen, Emel; Arslanyolu, Muhittin
Source:
Zeitschrift für Naturforschung 2014 v.65 no.3-4 pp. 266-270
ISSN:
1865-7125
Subject:
isothiocyanates; Origanum onites; ambient temperature; guanidines; medicinal plants; isopropyl alcohol; ultracentrifugation; reverse transcription; RNA; tissues; leaves; phenolic compounds
Abstract:
... We successfully used the guanidine isothiocyanate method for isolation of total RNA from leaf, stem, and root tissues of the aromatic plant Origanum onites. The RNA was extracted with TRI Reagent® at room temperature and was recovered by isopropanol precipitation. The isolated RNA was capable of reverse transcription. The extraction method described here does not require ultracentrifugation, and i ...
DOI:
10.1515/znc-2010-3-415

13. Molecular Cloning, Expression and Characterization of BmIDGF Gene from Bombyx mori

Author:
Pan, Ye; Chen, Keping; Xia, Hengchuan; Yao, Qin; Gao, Lu; Lü, Peng; Huojuan,; He, Yuanqing; Wang, Lin
Source:
Zeitschrift für Naturforschung 2014 v.65 no.3-4 pp. 277-283
ISSN:
1865-7125
Subject:
extracellular space; invertebrates; Escherichia coli; growth factors; sequence alignment; polyacrylamide gel electrophoresis; reverse transcriptase polymerase chain reaction; Western blotting; insect larvae; mass spectrometry; Bombyx mori; molecular cloning; insect development; enzyme activity; exons; introns; chitinase
Abstract:
... Imaginal disc growth factors (IDGF) play a key role in insect development, but their mechanism remains unclear. In this study, we cloned a novel IDGF gene in Bombyx mori and designated it as BmIDGF. We found that the BmIDGF gene contains eight exons and seven introns, encoding a peptide of 434 amino-acid residues. The protein was predicted to contain one conserved motif of the glycosyl hydrolases ...
DOI:
10.1515/znc-2010-3-417

14. n-Alkylresorcinol Occurrence in Mercurialis perennis L. (Euphorbiaceae)

Author:
Lorenz, Peter; Knödler, Matthias; Bertrams, Julia; Berger, Melanie; Meyer, Ulrich; Stintzing, Florian C.
Source:
Zeitschrift für Naturforschung 2014 v.65 no.3-4 pp. 174-179
ISSN:
1865-7125
Subject:
Mercurialis perennis; gas chromatography-mass spectrometry; methylene chloride; pharmacognosy; plant biochemistry; roots
Abstract:
... Investigation of the dichloromethane extracts from herbal and root parts of Mercurialis perennis L. afforded a mixture of 11 homologous n-alkylresorcinols (ARs) with saturated odd-numbered alkyl side chains (C15:0-C27:0). In addition to three predominant ARs (C19:0, C21:0 and C23:0), a number of minor ARs were identified by use of LC-MS/MS and GC-MS techniques. Among the compounds detected, four u ...
DOI:
10.1515/znc-2010-3-402

15. Nematicidal Activity of Beauvericin Produced by the Fungus Fusarium bulbicola

Author:
Shimada, Atsumi; Fujioka, Shozo; Koshino, Hiroyuki; Kimura, Yasuo
Source:
Zeitschrift für Naturforschung 2014 v.65 no.3-4 pp. 207-210
ISSN:
0939-5075
Subject:
Bursaphelenchus xylophilus; Caenorhabditis elegans; Fusarium; Pinus; fungi; nematicidal properties; nematicides; spectral analysis
Abstract:
... A nematicide, beauvericin (1), was isolated from cultures of the fungus Fusarium bulbicola, and its structure was identified by spectroscopic analysis. Compound 1 showed nematicidal activities against the pine wood nematode Bursaphelenchus xylophilus and the freeliving nematode Caenorhabditis elegans. ...
DOI:
10.1515/znc-2010-3-407

16. On the Disruption of Cell Multiplication by Oscillating Electric Fields

Author:
Winterberg, Friedwardt
Source:
Zeitschrift für Naturforschung 2014 v.65 no.3-4 pp. 307-308
ISSN:
1865-7125
Subject:
electric field; magnetic fields; neoplasms
Abstract:
... This communication is a theoretical consideration explaining the benefits to reduce tumour growth in situ by interaction of ultrasound and strong magnetic fields. No documentation is presented. ...
DOI:
10.1515/znc-2010-3-421

17. Physicochemical Characteristics of a Thermostable Gellan Lyase from Geobacillus stearothermophilus 98

Author:
Derekova, Anna; Atanassova, Miroslava; Christova, Petya; Tchorbanov, Bojidar; Shosheva, Alexandra; Mandeva, Rossitsa; Rodríguez-Alonso, Patricia; Garabal, Jose I.; Kambourova, Margarita
Source:
Zeitschrift für Naturforschung 2014 v.65 no.3-4 pp. 231-238
ISSN:
1865-7125
Subject:
Geobacillus stearothermophilus; amino acids; circular dichroism spectroscopy; crystals; denaturation; differential scanning calorimetry; gel electrophoresis; heat production; molecular weight; thermal stability; thermophilic bacteria
Abstract:
... A purified thermostable gellan lyase, produced by a thermophilic bacterium, Geobacillus stearothermophilus 98, was characterized in relation to its physicochemical properties. The gellan lyase was established to have a molecular weight of 216 kDa, defined by capillary gel electrophoresis. Amino acid analysis revealed high quantities of Lys, His, Ala, Val, Ile, Glx, and Pro residues. The circular d ...
DOI:
10.1515/znc-2010-3-411

18. Polymorphism of Insulin-Like Growth Factor I Gene among Chicken Breeds in Egypt

Author:
Gouda, Eman M.; Essawy, Gamal S.
Source:
Zeitschrift für Naturforschung 2014 v.65 no.3-4 pp. 284-288
ISSN:
1865-7125
Subject:
cell proliferation; chicken breeds; chickens; genes; genotype; growth traits; insulin-like growth factor I; liver; messenger RNA; phenotype; protein synthesis; reverse transcriptase polymerase chain reaction; single-stranded conformational polymorphism; Egypt
Abstract:
... The insulin-like growth factor I (IGF-I) regulates growth, protein synthesis, and cell proliferation and differentiation in vertebrates. Polymorphisms of IGF-I gene transcripts of three breeds of chicken were assessed. The associations of these polymorphisms with the growth rate of the studied breeds were also evaluated. Total RNA was isolated from chicken livers, and the IGF-I gene was amplified ...
DOI:
10.1515/znc-2010-3-418

19. Possible Fungistatic Implications of Betulin Presence in Betulaceae Plants and their Hymenochaetaceae Parasitic Fungi

Author:
Jasicka-Misiak, Izabela; Lipok, Jacek; Świder, Izabela A.; Kafarski, Paweł
Source:
Zeitschrift für Naturforschung 2014 v.65 no.3-4 pp. 201-206
ISSN:
1865-7125
Subject:
Betula; Fourier transform infrared spectroscopy; Hymenochaetaceae; antifungal properties; bark; betulin; betulinic acid; bioassays; cosmetics; emulsifiers; emulsifying; emulsions; fungi; gas chromatography-mass spectrometry; median effective concentration; medicine; microbial growth; nuclear magnetic resonance spectroscopy; nutmeg; pharmaceutical industry; trees; ultrasonics
Abstract:
... Betulin and its derivatives (especially betulinic acid) are known to possess very interesting prospects for their application in medicine, cosmetics and as bioactive agents in pharmaceutical industry. Usually betulin is obtained by extraction from the outer layer of a birch bark. In this work we describe a simple method of betulin isolation from bark of various species of Betulaceae trees and para ...
DOI:
10.1515/znc-2010-3-406

20. Progesterone and β-Estradiol Stimulate Seed Germination in Chickpea by Causing Important Changes in Biochemical Parameters

Author:
Erdal, Serkan; Dumlupinar, Rahmi
Source:
Zeitschrift für Naturforschung 2014 v.65 no.3-4 pp. 239-244
ISSN:
0939-5075
Subject:
Cicer arietinum; alpha-amylase; biochemical pathways; catalase; chickpeas; enzyme activity; hydrogen peroxide; lipid peroxidation; peroxidase; progesterone; roots; seed germination; seeds; shoots; superoxide dismutase
Abstract:
... Effects of progesterone and β-estradiol on morphologic (germination velocity, root and shoot length) and biochemical (activities of α-amylase, superoxide dismutase, peroxidase and catalase, H₂O₂ content, lipid peroxidation) parameters during germination and post-germination stages of chickpea seeds were studied. The seeds germinated at various hormone concentrations (10 ⁻ ⁴, 10 ⁻ ⁶, 10 ⁻ ⁹, 10 ⁻ ¹ ...
DOI:
10.1515/znc-2010-3-412