Author: "Rode, Kristina" / Publication Year: 2020 / Subject: Sertoli cells and polymerase chain reaction

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Author:: Rode, Kristina, et al. ; Gerber, Jonathan; Hambruch, Nina; Langeheine, Marion; Schnepel, Nadine; Brehm, Ralph; Show all 6 Authors
Source:: Cell and tissue research 2020 v.381 no.2 pp. 309-326
ISSN:: 0302-766X
Subject:: Sertoli cells; Western blotting; assays; cell culture; cell lines; cell proliferation; dynamics; electrical resistance; fluorescent antibody technique; gap junctions; genotype; immunohistochemistry; mice; polymerase chain reaction; research; seminiferous epithelium; spermatogenesis; tight junctions
Abstract:: ... The Sertoli cell (SC) specific connexin43 (Cx43) knockout (SCCx43KO) mouse line is ideal to gain insight into the mechanistic gap junction formation in SC and the seminiferous epithelium. A method for developing primary SC cultures from these mice was established, validated and successfully characterized via polymerase chain reaction, immunohistochemistry, immunofluorescence (IF), and Western blot ...
DOI:: 10.1007/s00441-020-03203-y
: http://dx.doi.org/10.1007/s00441-020-03203-y