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You searched for: Publication year rev 7980-2020 Remove constraint Publication year rev: 7980-2020 Subject term Shiga toxin-producing Escherichia coli Remove constraint Subject term: Shiga toxin-producing Escherichia coli Text availability Citation in PubAg Remove constraint Text availability: Citation in PubAg
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1. A Causal Long Short-Term Memory Sequence to Sequence Model for TEC Prediction Using GNSS Observations

Author:
Maria Kaselimi; Athanasios Voulodimos; Nikolaos Doulamis; Anastasios Doulamis; Demitris Delikaraoglou
Source:
Remote Sensing 2020 v.12 no.9 pp. -
ISSN:
2072-4292
Subject:
Shiga toxin-producing Escherichia coli; equipment; neural networks; prediction; remote sensing; satellites; time series analysis
Abstract:
... The necessity of predicting the spatio-temporal phenomenon of ionospheric variability is closely related to the requirement of many users to be able to obtain high accuracy positioning with low cost equipment. The Precise Point Positioning (PPP) technique is highly accepted by the scientific community as a means for providing high level of position accuracy from a single receiver. However, its mai ...
DOI:
10.3390/rs12091354

2. A Practical Method to Implement Strain-Level Metagenomics-Based Foodborne Outbreak Investigation and Source Tracking in Routine

Author:
Florence E. Buytaers; Assia Saltykova; Sarah Denayer; Bavo Verhaegen; Kevin Vanneste; Nancy H. C. Roosens; Denis Piérard; Kathleen Marchal; Sigrid C. J. De Keersmaecker
Source:
Microorganisms 2020 v.8 no.8 pp. -
ISSN:
2076-2607
Subject:
DNA; Shiga toxin-producing Escherichia coli; case studies; enzymes; food matrix; food pathogens; foods; genome; human diseases; metagenomics; outbreak investigation; patients; sequence analysis
Abstract:
... The management of a foodborne outbreak depends on the rapid and accurate identification of the responsible food source. Conventional methods based on isolation of the pathogen from the food matrix and target-specific real-time polymerase chain reactions (qPCRs) are used in routine. In recent years, the use of whole genome sequencing (WGS) of bacterial isolates has proven its value to collect relev ...
DOI:
10.3390/microorganisms8081191

3. A pilot study of spiral-wound air gap membrane distillation process and its energy efficiency analysis

Author:
Chang-Kyu Lee; Chansoo Park; Yun Chul Woo; June-Seok Choi; Jong-Oh Kim
Source:
Chemosphere 2020 v.239 pp. 124696
ISSN:
0045-6535
Subject:
Shiga toxin-producing Escherichia coli; air; cooling; desalination; distillation; energy efficiency; heat; reverse osmosis; saline water; seawater; temperature; thermal energy; water vapor
Abstract:
... Brine disposal is a major drawback for seawater desalination. Membrane distillation (MD) is an emerging technology to treat a high saline water including brine disposal instead of reverse osmosis, multi-stage flash and multi-effect distillation. This study investigated a pilot scale of a spiral-wound air gap MD (AGMD) module and evaluated its efficiency. A pilot-scale AGMD module with design produ ...
DOI:
10.1016/j.chemosphere.2019.124696

4. A qualitative risk assessment approach to microbial foodborne hazards in Brazilian intensive pork production: A step towards risk prioritization

Author:
Eduardo de Freitas Costa; Marisa Cardoso; Jalusa Deon Kich; Luis Gustavo Corbellini
Source:
Microbial risk analysis 2020 v.15 pp. 100105
ISSN:
2352-3522
Subject:
Codex Alimentarius; Salmonella; Shiga toxin-producing Escherichia coli; adverse effects; biological hazards; biosecurity; exposure assessment; farms; foodborne illness; hazard characterization; hazard identification; herds; humans; microbiological risk assessment; models; pig carcasses; pork; prioritization; qualitative risk assessment; risk; risk assessment process; risk characterization; risk profile; swine; uncertainty; zoonoses; Brazil
Abstract:
... The application of biosecurity measures in pig farms has prompted a shift in epidemiological reality, altering the risk profile attributed to the consumption of pork. The inspection of pig carcasses in Brazil is based on incisions and palpations to seek lesions mainly related to classic zoonotic diseases. We describe the use of a qualitative risk assessment from the farm to the table to characteri ...
DOI:
10.1016/j.mran.2020.100105

5. Adaptive Modeling of the Global Ionosphere Vertical Total Electron Content

Author:
Eren Erdogan; Michael Schmidt; Andreas Goss; Barbara Görres; Florian Seitz
Source:
Remote Sensing 2020 v.12 no.11 pp. -
ISSN:
2072-4292
Subject:
Shiga toxin-producing Escherichia coli; algorithms; altimetry; covariance; data collection; disasters; dynamic models; model uncertainty; monitoring; remote sensing; stochastic processes; temporal variation; variance; weather
Abstract:
... The Kalman filter (KF) is widely applied in (ultra) rapid and (near) real-time ionosphere modeling to meet the demand on ionosphere products required in many applications extending from navigation and positioning to monitoring space weather events and naturals disasters. The requirement of a prior definition of the stochastic models attached to the measurements and the dynamic models of the KF is ...
DOI:
10.3390/rs12111822

6. Agricultural intensification heightens food safety risks posed by wild birds

Author:
Olivia M. Smith; Amanda Edworthy; Joseph M. Taylor; Matthew S. Jones; Aaron Tormanen; Christina M. Kennedy; Zhen Fu; Christopher E. Latimer; Kevin A. Cornell; Lucas A. Michelotti; Chika Sato; Tobin Northfield; William E. Snyder; Jeb P. Owen
Source:
Journal of applied ecology 2020 v.57 no.11 pp. 2246-2257
ISSN:
0021-8901
Subject:
Campylobacter; Salmonella; Shiga toxin-producing Escherichia coli; animal wastes; applied ecology; coasts; feces; food production; food safety; habitats; intensive farming; landscapes; livestock; mammals; uncertainty; wild birds; wildlife management
Abstract:
... Agricultural intensification and simplification are key drivers of recent declines in wild bird populations, heightening the need to better balance conservation with food production. This is hindered, however, by perceptions that birds threaten food safety. While birds are known reservoirs of foodborne pathogens, there remains uncertainty about the links between landscape context, farming practice ...
DOI:
10.1111/1365-2664.13723

7. An Extended-Spectrum Beta-Lactamase-Producing Hybrid Shiga-Toxigenic and Enterotoxigenic Escherichia coli Strain Isolated from a Piglet with Diarrheal Disease in Northeast China

Author:
Xue Ji; Bing Liang; Yang Sun; Lingwei Zhu; Bo Zhou; Xuejun Guo; Jun Liu
Source:
Foodborne pathogens & disease 2020 v.17 no.6 pp. 382-387
ISSN:
1556-7125
Subject:
Escherichia infections; Shiga toxin-producing Escherichia coli; ancestry; birds; diagnostic techniques; diarrhea; enterotoxigenic Escherichia coli; farms; genes; hybrids; livestock and meat industry; monitoring; nucleotide sequences; pathogens; phylogeny; piglets; plasmids; public health; toxigenic strains; virulence; virulent strains; China
Abstract:
... Shiga-toxigenic Escherichia coli (STEC) and enterotoxigenic E. coli (ETEC) can cause diarrhea in piglets. This is the first report and complete genome sequence of an extended-spectrum β-lactamase-producing hybrid STEC/ETEC strain isolated from a piglet with diarrhea on a swine farm in China. We investigated the virulence genes and phylogenetic diversity with publicly available E. coli genomes. Bot ...
DOI:
10.1089/fpd.2019.2720

8. An Overview of the Elusive Passenger in the Gastrointestinal Tract of Cattle: The Shiga Toxin Producing Escherichia coli

Author:
Panagiotis Sapountzis; Audrey Segura; Mickaël Desvaux; Evelyne Forano
Source:
Microorganisms 2020 v.8 no.6 pp. -
ISSN:
2076-2607
Subject:
Shiga toxin; Shiga toxin-producing Escherichia coli; animal husbandry; cattle; diet; digestive tract; intestinal microorganisms; meat; temperature
Abstract:
... For approximately 10,000 years, cattle have been our major source of meat and dairy. However, cattle are also a major reservoir for dangerous foodborne pathogens that belong to the Shiga toxin-producing Escherichia coli (STEC) group. Even though STEC infections in humans are rare, they are often lethal, as treatment options are limited. In cattle, STEC infections are typically asymptomatic and STE ...
DOI:
10.3390/microorganisms8060877

9. An Stx-EAEC O59:NM[H19] strain isolated from a hemolytic uremic syndrome case in Argentina

Author:
Claudia C. Carbonari; Miriam Ricciardi; Ana Rodríguez Calvo; Alejandro Montes; Natalia L. Deza; María A. Conde Valentino; Gisela Zolezzi; Ariela Baschkier; Mariano Vago; Denise Acosta; Eduardo A. Manfredi; Elizabeth S. Miliwebsky; Isabel Chinen; Marta Rivas
Source:
Revista argentina microbiología 2020 v.52 no.1 pp. 31-35
ISSN:
0325-7541
Subject:
Shiga toxin-producing Escherichia coli; children; diarrhea; hemolytic uremic syndrome; human diseases; monitoring; streptomycin; toxicity; trimethoprim; Argentina; Germany
Abstract:
... Shiga toxin-producing Escherichia coli (STEC) are a heterogeneous group of foodborne pathogens causing a broad spectrum of human disease, from uncomplicated diarrhea to hemolytic uremic syndrome (HUS). In this study, we report an HUS case associated with an O59:NM[H19] strain, harboring stx₂ₐ, iha, lpfAO₂₆, lpfAO₁₁₃ genes associated with STEC, and aatA, aap, pic, sigA, agg4A genes associated with ...
DOI:
10.1016/j.ram.2019.03.006

10. Analysis of Bacterial Diversity in Relation to the Presence of the Top 7 Shiga Toxin–Producing Escherichia coli throughout Australian Beef Abattoirs

Author:
Sanga Kang; Joshua T. Ravensdale; Ranil Coorey; Gary A. Dykes; Robert S. Barlow
Source:
Journal of food protection 2020 v.83 no.10 pp. 1812-1821
ISSN:
0362-028X
Subject:
Food Safety and Inspection Service; Shiga toxin-producing Escherichia coli; beef; beef cattle; food safety; slaughter; slaughterhouses; species diversity; supply chain
Abstract:
... There is increasing evidence that diversity changes in bacterial communities of beef cattle correlate to the presence of Shiga toxin–producing Escherichia coli (STEC). However, studies that found an association between STEC and bacterial diversity have been focused on preslaughter stages in the beef supply chain. This study was designed to test a hypothesis that there are no differences in bacteri ...
DOI:
10.4315/JFP-20-109

11. Antimicrobial resistance among canine enteric Escherichia coli isolates and prevalence of attaching–effacing and extraintestinal pathogenic virulence factors in Spain

Author:
Eloisa Sevilla; Raúl C. Mainar-Jaime; Bernardino Moreno; Inmaculada Martín-Burriel; Mariano Morales; Sara Andrés-Lasheras; Manuel Chirino-Trejo; Juan J. Badiola; Rosa Bolea
Source:
Acta veterinaria Hungarica 2020 v. no. pp. -
ISSN:
1588-2705
Subject:
Shiga toxin-producing Escherichia coli; aminoglycosides; antibiotic resistance; beta-lactamase; cephalosporins; colistin; dogs; enteropathogenic Escherichia coli; extraintestinal pathogenic Escherichia coli; feces; genes; humans; multiple drug resistance; pathogens; penicillins; polymerase chain reaction; risk; virulence; Spain
Abstract:
... The aim of this study was to estimate the prevalence of antimicrobial resistance (AMR) in Escherichia coli from a dog population in Spain and assess specific virulence factors. Susceptibility to 22 antimicrobials was tested along with the production of extended-spectrum β-lactamases (ESBLs) and AmpC in faecal isolates from 100 dogs. Virulence-related genes associated with attaching and effacing E. ...
DOI:
10.1556/004.2020.00013

12. Application of chlorine dioxide and peroxyacetic acid during spray chilling as a potential antimicrobial intervention for beef carcasses

Author:
Chawalit Kocharunchitt; Lyndal Mellefont; John P. Bowman; Tom Ross
Source:
Food microbiology 2020 v.87 pp. 103355
ISSN:
0740-0020
Subject:
Salmonella enterica; Shiga toxin-producing Escherichia coli; antimicrobial properties; beef; beef carcasses; beef industry; chlorine dioxide; cold treatment; enteropathogens; food safety; oxidants; oxidative stress; peracetic acid
Abstract:
... Enteric pathogens such as Shiga-toxin producing Escherichia coli (STEC) and Salmonella spp. continue to be a major food safety concern for the beef industry. Currently, no single method is completely effective in controlling these pathogens during carcass processing. Previous research, however, suggested that STEC might become more susceptible to oxidative damage when exposed to carcass chilling ( ...
DOI:
10.1016/j.fm.2019.103355

13. Assessment and Comparison of Broadcast Ionospheric Models: NTCM-BC, BDGIM, and Klobuchar

Author:
Chao Yang; Jing Guo; Tao Geng; Qile Zhao; Kecai Jiang; Xin Xie; Yifei Lv
Source:
Remote Sensing 2020 v.12 no.7 pp. -
ISSN:
2072-4292
Subject:
Shiga toxin-producing Escherichia coli; electrons; global positioning systems; models; remote sensing; satellites; China; Europe
Abstract:
... For single-frequency Global Navigation Satellite Systems (GNSSs) users, ionospheric delay is the main error source affecting the accuracy of positioning. Applying a broadcast ionospheric correction model to mitigate the ionospheric delay is essential for meter-to-decimeter-level accuracy positioning. To provide support for real-time single-frequency operations, particularly in the China area, we a ...
DOI:
10.3390/rs12071215

14. Association of Ct Values from Real-Time PCR with Culture in Microbiological Clearance Samples for Shiga Toxin-Producing Escherichia coli (STEC)

Author:
Michael Bording-Jorgensen; Brendon D. Parsons; Gillian A.M. Tarr; Binal Shah-Gandhi; Colin Lloyd; Linda Chui
Source:
Microorganisms 2020 v.8 no.11 pp. -
ISSN:
2076-2607
Subject:
DNA; Shiga toxin-producing Escherichia coli; agar; detection; diagnostic techniques; feces; frequency; gastroenteritis; genes; health services; laboratories; microorganisms; monitoring; objectives; quantitative polymerase chain reaction; sampling; Alberta
Abstract:
... Shiga toxin-producing Escherichia coli (STEC) are associated with acute gastroenteritis worldwide, which induces a high economic burden on both healthcare and individuals. Culture-independent diagnostic tests (CIDT) in frontline microbiology laboratories have been implemented in Alberta since 2019. The objectives of this study were to determine the association between gene detection and culture po ...
DOI:
10.3390/microorganisms8111801

15. Associations Between Season, Processing Plant, and Hide Cleanliness Scores with Prevalence and Concentration of Major Shiga Toxin–Producing Escherichia coli on Beef Cattle Hides

Author:
Natalia Cernicchiaro; Ana R.S. Oliveira; Allison Hoehn; Lance W. Noll; Pragathi B. Shridhar; Tiruvoor G. Nagaraja; Samuel E. Ives; David G. Renter; Michael W. Sanderson
Source:
Foodborne pathogens & disease 2020 pp. -
ISSN:
1556-7125
Subject:
Shiga toxin-producing Escherichia coli; autumn; beef carcasses; beef cattle; confidence interval; meat processing plants; serotypes; spring; standard deviation; summer; United States
Abstract:
... The objectives of this study were (1) to estimate the prevalence and concentration of the seven major Shiga toxin–producing Escherichia coli (STEC) serogroups (O26, O45, O103, O111, O121, O145, and O157), collectively called STEC-7, on cattle hides collected in different seasons and beef processing plants; and (2) to determine associations of season, plant, and hide cleanliness scores with the pre ...
DOI:
10.1089/fpd.2019.2778

16. Associations of five food- and water-borne diseases with ecological zone, land use and aquifer type in a changing climate

Author:
Jordan Brubacher; Diana M. Allen; Stephen J. Déry; Margot W. Parkes; Bimal Chhetri; Sunny Mak; Stephen Sobie; Tim K. Takaro
Source:
Science of the total environment 2020 v.728 pp. 138808
ISSN:
0048-9697
Subject:
Protozoa; Shiga toxin-producing Escherichia coli; agricultural land; aquifers; campylobacteriosis; climate change; cryptosporidiosis; ecosystems; gastrointestinal system; giardiasis; land use; regression analysis; relative risk; salmonellosis; spatial variation; temperature; British Columbia
Abstract:
... Food- and water-borne pathogens exhibit spatial heterogeneity, but attribution to specific environmental processes is lacking while anthropogenic climate change alters these processes. The goal of this study was to investigate ecology, land-use and health associations of these pathogens and to make future disease projections.The rates of five acute gastrointestinal illnesses (AGIs) (campylobacteri ...
DOI:
10.1016/j.scitotenv.2020.138808

17. Bacteriophage biocontrol of Shiga toxigenic Escherichia coli (STEC) O145 biofilms on stainless steel reduces the contamination of beef

Author:
Changbao Wang; Hua Hang; Shoubiao Zhou; Yan D. Niu; Hechao Du; Kim Stanford; Tim A. McAllister
Source:
Food microbiology 2020 v.92 pp. 103572
ISSN:
0740-0020
Subject:
Shiga toxin-producing Escherichia coli; bacteriophages; beef; biofilm; biological control; food industry; food microbiology; plankton; stainless steel; viability
Abstract:
... Shiga toxigenic Escherichia coli (STEC) can form biofilms and frequently cause serious foodborne illnesses. A strain of STEC O145:H25 (EC19990166) known to be a strong biofilm former was used to evaluate the efficacy of bacteriophage AZO145A against biofilms formed on stainless steel (SS) coupons. Exposure of STEC O145:H25 to phage AZO145A (10¹⁰ PFU/mL) for 2 h resulted in a 4.0 log₁₀ reduction (P ...
DOI:
10.1016/j.fm.2020.103572

18. Bacteriophages specific to Shiga toxin-producing Escherichia coli exist in goat feces and associated environments on an organic produce farm in Northern California, USA

Author:
Lennon Marion; Liao Yen; Salvador Alexandra; Lauzon Carol R.; Wu Vivian Chi Hua
Source:
PloS one 2020 v.15 no. pp. -
ISSN:
1932-6203
Subject:
Myoviridae; Podoviridae; Shiga toxin; Shiga toxin-producing Escherichia coli; Siphoviridae; bacteriophages; biological control; cattle; farms; feces; foodborne illness; genes; goats; host range; organic foods; polymerase chain reaction; soil; California
Abstract:
... Shiga toxin-producing Escherichia coli (STEC) contamination on produce is primarily associated with ruminant feces, which can result in severe foodborne illness. Although numerous STEC-specific bacteriophages (phages) have been isolated from cattle feces, the presence of STEC-specific phages and any correlation with O157 and the top six non-O157 STECs (the top seven STECs) in goat feces have not b ...
Handle:
10113/7019775
DOI:
10.1371/journal.pone.0234438

19. Bacteriophages to control Shiga toxin-producing E. coli – safety and regulatory challenges

Author:
Graça Pinto; Carina Almeida; Joana Azeredo
Source:
Critical reviews in biotechnology 2020 v.40 no.8 pp. 1081-1097
ISSN:
1549-7801
Subject:
Escherichia infections; European Union; Shiga toxin-producing Escherichia coli; antibiotics; biological control agents; coliphages; decontamination; disease control; food industry; food law; food safety; foodborne bacterial pathogens; guidelines; virulence
Abstract:
... Shiga toxin-producing Escherichia coli (STEC) are usually found on food products due to contamination from the fecal origin, as their main environmental reservoir is considered to be the gut of ruminants. While this pathogen is far from the incidence of other well-known foodborne bacteria, the severity of STEC infections in humans has triggered global concerns as far as its incidence and control a ...
DOI:
10.1080/07388551.2020.1805719

20. Behaviour of Non-O157 STEC and Atypical EPEC during the Manufacturing and Ripening of Raw Milk Cheese

Author:
Edson A. Rios; Juliana Ramos-Pereira; Jesús A. Santos; Teresa M. López-Díaz; Andrés Otero; Jose M. Rodríguez-Calleja
Source:
Foods 2020 v.9 no.9 pp. -
ISSN:
2304-8158
Subject:
Shiga toxin-producing Escherichia coli; behavior; cheesemaking; cross contamination; dairy industry; enteropathogenic Escherichia coli; hard cheeses; manufacturing; microbiological quality; milk; pathotypes; population; pressing; raw milk; ripening; sampling; serotypes
Abstract:
... This study was carried out to assess the survival of Shiga toxin-producing E. coli (STEC) and atypical enteropathogenic Escherichia coli (aEPEC) during the traditional manufacturing and ripening of Spanish hard cheese from raw cow’s milk. Milk samples were spiked with up to 3.1–3.5 log cfu/mL of one strain of STEC (O140:H32 serotype) and one of aEPEC (serotype O25:H2). The first steps of cheesemak ...
DOI:
10.3390/foods9091215

21. Biofilm formation by South African non-O157 Shiga toxigenic Escherichiacoli on stainless steel coupons

Author:
Emmanuel W. Bumunang; Collins N. Ateba; Kim Stanford; Tim A. McAllister; Yan D. Niu
Source:
Canadian journal of microbiology 2020 v.66 no.4 pp. 328-336
ISSN:
1480-3275
Subject:
Shiga toxin-producing Escherichia coli; adulterated products; biofilm; cell viability; food pathogens; food processing; food processing plants; gentian violet; liquid-air interface; scanning electron microscopy; staining; stainless steel; temperature; toxigenic strains
Abstract:
... This study examined the biofilm-forming ability of six non-O157 Shiga-toxin-producing Escherichia coli (STEC) strains: O116:H21, wzx-Onovel5:H19, O129:H21, O129:H23, O26:H11, and O154:H10 on stainless steel coupons after 24, 48, and 72 h of incubation at 22 °C and after 168 h at 10 °C. The results of crystal violet staining revealed that strains O129:H23 and O154:H10 were able to form biofilms on ...
DOI:
10.1139/cjm-2019-0554

22. Bovine lymph nodes as a source of Escherichia coli contamination of the meat

Author:
Luca Grispoldi; Musafiri Karama; Chrystalleni Hadjicharalambous; Fabrizio de Stefani; Giulia Ventura; Margherita Ceccarelli; Marco Revoltella; Paola Sechi; Carlo Crotti; Antonio D'Innocenzo; Gerardo Couto-Contreras; Beniamino Cenci-Goga
Source:
International journal of food microbiology 2020 v.331 pp. 108715
ISSN:
0168-1605
Subject:
Shiga toxin-producing Escherichia coli; ampicillin; antibiotic resistance; beef carcasses; cattle; ceftriaxone; cephalothin; evisceration; genes; gentamicin; good manufacturing practices; ground beef; intestines; lymph nodes; manufacturing; meat production; multivariate analysis; polymerase chain reaction; risk assessment; slaughterhouses; streptomycin; tetracycline; tripe; Italy
Abstract:
... Ground beef contamination with Escherichia coli is usually a result of carcass faecal contamination during the slaughter process. Carcasses are contaminated when they come into contact with soiled hides or intestinal leakage content during dressing and the evisceration processes. A more recent and compelling hypothesis is that, when lymph nodes are present in manufacturing beef trimmings, they can ...
DOI:
10.1016/j.ijfoodmicro.2020.108715

23. Burden of Disease Estimates of Seven Pathogens Commonly Transmitted Through Foods in Denmark, 2017

Author:
Sara Monteiro Pires; Lea Sletting Jakobsen; Johanne Ellis-Iversen; Joana Pessoa; Steen Ethelberg
Source:
Foodborne pathogens & disease 2020 v.17 no.5 pp. 322-339
ISSN:
1556-7125
Subject:
Campylobacter; Listeria monocytogenes; Norovirus; Salmonella; Shiga toxin-producing Escherichia coli; Toxoplasma gondii; Yersinia enterocolitica; campylobacteriosis; disability-adjusted life year; exposure pathways; food chain; food pathogens; food safety; foods; monitoring; mortality; public health; toxoplasmosis; uncertainty; Denmark
Abstract:
... Burden of disease metrics are increasingly established to prioritize food safety interventions. We estimated the burden of disease caused by seven foodborne pathogens in Denmark in 2017: Campylobacter, Salmonella, Shiga toxin–producing Escherichia coli, norovirus, Yersinia enterocolitica, Listeria monocytogenes, and Toxoplasma gondii. We used public health surveillance data and scientific literatu ...
DOI:
10.1089/fpd.2019.2705

24. Caracterización molecular de aislamientos de Escherichia coli productores de toxina Shiga obtenidos en 2 establecimientos ganaderos del Paraguay

Author:
Sofía M. Rivelli Zea; Nora L. Padola; Analía I. Etcheverría; Melisa Florentín; Patricia Acuña; Fátima Rodríguez; Rocío Colello; Rosa M. Guillén Fretes
Source:
Revista argentina microbiología 2020 v.52 no.2 pp. 131-135
ISSN:
0325-7541
Subject:
Shiga toxin-producing Escherichia coli; agglutination; cattle; food pathogens; serotypes; virulence; Paraguay
Abstract:
... Shiga toxin-producing Escherichia coli (STEC) is a food-borne pathogen in humans, with cattle being the main reservoir. The objective of this study was to determine the carrying of STEC in Paraguayan bovines and to analyze the virulence profile and serotypes of these isolates. A total of 197 samples of bovine fecal samples and an average of 5 to 50 colonies from stx₁/stx₂ positive samples were stu ...
DOI:
10.1016/j.ram.2019.07.001

25. Characterization and molecular subtyping of Shiga toxin–producing Escherichia coli strains in provincial abattoirs from the Province of Buenos Aires, Argentina, during 2016-2018

Author:
Magdalena Costa; Alejandra Londero; Victoria Brusa; Lucía Galli; Claudia Van Der Ploeg; Ariel Roge; Gerardo A. Leotta
Source:
Preventive veterinary medicine 2020 v.183 pp. 105133
ISSN:
0167-5877
Subject:
Shiga toxin-producing Escherichia coli; animal organs; cold treatment; cross contamination; food safety; genes; good hygiene practices; good manufacturing practices; head; meat; pulsed-field gel electrophoresis; restriction endonucleases; risk reduction; slaughterhouses; washing; Argentina
Abstract:
... We characterized Shiga toxin–producing Escherichia coli (STEC) O157 (n = 20) and non-O157 (n = 68) isolated from carcasses (n = 54), the environment (n = 20), head meat (n = 3) and viscera washing and chilling water (n = 11) in provincial abattoirs before and after implementing improvement actions. The strains were tested for eae, saa, ehxA and fliCH₇ genes. Variants stx₁ and stx₂ were also determ ...
DOI:
10.1016/j.prevetmed.2020.105133

26. Characterization of virulence genes in Escherichia coli strains isolated from pre-weaned calves in the Republic of Korea

Author:
Ji-Hyoung Ryu; SuHee Kim; Jinho Park; Kyoung-Seong Choi
Source:
Acta veterinaria scandinavica 2020 v.62 no.1 pp. 45
ISSN:
1751-0147
Subject:
Shiga toxin; Shiga toxin-producing Escherichia coli; calves; diarrhea; enterotoxigenic Escherichia coli; genes; pathotypes; polymerase chain reaction; risk; virulence; South Korea
Abstract:
... BACKGROUND: Escherichia coli is an important cause of diarrhea in calves and its diarrheagenic properties are related to presence of certain virulence genes. In this study, the prevalence of virulence genes F5, F17, F41, sta, stx1, stx2, eae, and saa in E. coli isolated from pre-weaned calves presenting with (n= 329) or without diarrhea (n= 360) was explored using multiplex polymerase chain reacti ...
DOI:
10.1186/s13028-020-00543-1

27. Characterization of water treatment-resistant and multidrug-resistant urinary pathogenic Escherichia coli in treated wastewater

Author:
Shuai Zhi; Paul Stothard; Graham Banting; Candis Scott; Kristin Huntley; Kanghee Ryu; Simon Otto; Nicholas Ashbolt; Sylvia Checkley; Tao Dong; Norma J. Ruecker; Norman F. Neumann
Source:
Water research 2020 v.182 pp. 115827
ISSN:
0043-1354
Subject:
Shiga toxin-producing Escherichia coli; antibiotic resistance; beta-lactamase; chlorine; complement; genomics; multiple drug resistance; pathotypes; phenotype; public health; research; risk; sewage; sewage treatment; virulence; wastewater; wastewater treatment; water
Abstract:
... A growing body of evidence has demonstrated that extraintestinal pathogenic E. coli (ExPEC), such as the urinary pathogenic E. coli (UPEC), are common constituents of treated wastewater, and therefore represent a potential public health risk. However, no single virulence gene, or set of virulence genes, can be used to conclusively identify this genetically diverse pathotype. As such we sought to i ...
DOI:
10.1016/j.watres.2020.115827

28. Comparable stx2a expression and phage production levels between Shiga toxin‐producing Escherichia coli strains from human and bovine origin

Author:
Julia Burgán; Alejandra Krüger; Paula M. A. Lucchesi
Source:
Zoonoses and public health 2020 v.67 no.1 pp. 44-53
ISSN:
1863-1959
Subject:
Shiga toxin; Shiga toxin-producing Escherichia coli; cattle; diarrhea; disease severity; humans; mitomycin; pathogenesis; phages; quantitative polymerase chain reaction; serotypes; virulence
Abstract:
... Shiga toxin‐producing Escherichia coli (STEC) can cause diarrhoea and severe diseases in humans, such as haemolytic uraemic syndrome. STEC virulence is considered to correlate with the amount of Shiga toxins (Stx) produced, especially Stx2, whose subtype Stx2a is most frequently associated with high virulence. Stx are encoded in prophages, which play an important role in STEC pathogenesis. The aim ...
DOI:
10.1111/zph.12653

29. Comparison of the inhibitory potential of benzyl isothiocyanate and phenethyl isothiocyanate on Shiga toxin-producing and enterotoxigenic Escherichia coli

Author:
Chun-Xue Yang; Hai-Tao Wu; Xin-Xin Li; Hong-Yan Wu; Tong-Xin Niu; Xiao-Ning Wang; Rui Lian; Gong-Liang Zhang; Hong-Man Hou
Source:
Lebensmittel-Wissenschaft + Technologie 2020 v.118 pp. 108806
ISSN:
0023-6438
Subject:
Shiga toxin-producing Escherichia coli; adenosine triphosphate; antibacterial properties; benzyl isothiocyanate; dose response; enterotoxigenic Escherichia coli; gene expression regulation; genes; minimum inhibitory concentration; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; scanning electron microscopy; toxigenic strains; virulence
Abstract:
... The aim of this study was to investigate the inhibitory effects of benzyl isothiocyanate (BITC) and phenethyl isothiocyanate (PEITC) against Shiga toxin-producing Escherichia coli (STEC) and enterotoxigenic E. coli (ETEC). Compared with that of PEITC, the antibacterial effect of BITC was more obvious. The minimal inhibitory concentration (MIC) of BITC was 60 and 160 μmol/L for STEC and ETEC, while ...
DOI:
10.1016/j.lwt.2019.108806

30. Compatibility of Commercially Produced Protective Cultures with Common Cheesemaking Cultures and Their Antagonistic Effect on Foodborne Pathogens

Author:
Catherine A. Gensler; Stephanie R. B. Brown; Sulaiman F. Aljasir; Dennis J. D'Amico
Source:
Journal of food protection 2020 v.83 no.6 pp. 1010-1019
ISSN:
0362-028X
Subject:
Listeria monocytogenes; Salmonella; Shiga toxin-producing Escherichia coli; acidification; agar; antagonism; antimicrobial properties; cheese industry; cheeses; coculture; food safety; manufacturing; milk; temperature profiles
Abstract:
... The documented survival of pathogenic bacteria, including Listeria monocytogenes (LM), Shiga toxin–producing Escherichia coli (STEC), and Salmonella during the manufacture and aging of some cheeses highlights the need for additional interventions to enhance food safety. Unfortunately, few interventions are compliant with the Standards of Identity for cheese. Protective bacterial cultures (PCs) rep ...
DOI:
10.4315/JFP-19-614

31. Confounding Role of Salmonella Serotype Dublin Testing Results of Boneless and Ground Beef Purchased for the National School Lunch Program, October 2013 to July 2017

Author:
Scott L. Vial; Darin R. Doerscher; Carl M. Schroeder; Ali J. Strickland; Craig W. Hedberg
Source:
Journal of food protection 2020 v.83 no.4 pp. 628-636
ISSN:
0362-028X
Subject:
National School Lunch Program; Salmonella Dublin; Shiga toxin-producing Escherichia coli; coliform bacteria; ground beef; marketing; plate count; risk; sample size; serotypes; spring
Abstract:
... The Agricultural Marketing Service procures boneless and ground beef for federal nutrition assistance programs. It tests procured beef for concentrations of standard plate counts (SPCs), coliforms, and Escherichia coli and for the presence of Salmonella and Shiga toxin–producing E. coli. Any lot exceeding predefined critical limits (100,000 CFU g-1 for SPCs, 1,000 CFU g-1 for coli forms, and 500 C ...
DOI:
10.4315/0362-028X.JFP-19-359

32. Critical points affecting the microbiological safety of bell peppers washed with peroxyacetic acid in a commercial packinghouse

Author:
Francisco López-Gálvez; Pilar Truchado; Juan A. Tudela; Maria I. Gil; Ana Allende
Source:
Food microbiology 2020 v.88 pp. 103409
ISSN:
0740-0020
Subject:
Salmonella; Shiga toxin-producing Escherichia coli; bacteria; critical control points; cross contamination; hygiene; microbial load; microbiological quality; packing houses; peracetic acid; physicochemical properties; reflectometry; sweet peppers; virulent strains; washing
Abstract:
... The washing stage from a bell pepper commercial packinghouse was assessed to study some of the critical control points related to bacterial cross-contamination. The washing line comprised two overhead spray bars applications: a pre-wash step without peroxyacetic acid (PAA), and a wash step with PAA. The physicochemical characteristics of the wash water and the bacterial quality and safety of the w ...
DOI:
10.1016/j.fm.2019.103409

33. Deriving VTEC Maps from SMOS Radiometric Data

Author:
Roselena Rubino; Nuria Duffo; Verónica González-Gambau; Ignasi Corbella; Francesc Torres; Israel Durán; Manuel Martín-Neira
Source:
Remote Sensing 2020 v.12 no.10 pp. -
ISSN:
2072-4292
Subject:
Shiga toxin-producing Escherichia coli; Soil Moisture and Ocean Salinity satellite; databases; filters; models; radiometry; remote sensing; temperature
Abstract:
... In this work, a new methodology is proposed in order to derive vertical total electron content (VTEC) maps from the radiometric measurements of the Soil Moisture and Ocean Salinity (SMOS) mission as an alternative approach to those based on external databases and models. This approach uses spatiotemporal filtering techniques with optimized filters to be robust against the thermal noise and image r ...
DOI:
10.3390/rs12101604

34. Differential expression of the virulence gene nleB among Shiga toxin-producing Escherichia coli strains

Author:
Jimena S. Cadona; Julia Burgán; Juliana González; Ana V. Bustamante; A. Mariel Sanso
Source:
Heliyon 2020 v.6 no.6 pp. e04277
ISSN:
2405-8440
Subject:
Shiga toxin-producing Escherichia coli; cattle; food pathogens; gene expression regulation; genes; hemolytic uremic syndrome; inflammation; pathogenesis; public health; risk; serotypes; virulence; Argentina
Abstract:
... Shiga toxin-producing Escherichia coli (STEC) is a zoonotic foodborne pathogen associated with hemolytic uremic syndrome (HUS) that vary in their ability to cause disease in humans. STEC represents a serious problem for public health and Argentina is the country with the highest HUS incidence worldwide. Non-LEE effector (nle) genes, present on pathogenicity islands (PAIs), encode translocated subs ...
DOI:
10.1016/j.heliyon.2020.e04277

35. Distribution of Locus of Adhesion and Autoaggregation and hes Gene in STEC Strains from Countries of Latin America

Author:
María Victoria Vélez; Rocío Colello; Analía Inés Etcheverría; Roberto Mauricio Vidal; David Arturo Montero; Patricia Acuña; Rosa María Guillén Fretes; Magaly Toro; Nora Lía Padola
Source:
Current microbiology 2020 v.77 no.9 pp. 2111-2117
ISSN:
0343-8651
Subject:
Shiga toxin-producing Escherichia coli; adhesion; burden of disease; colitis; diarrhea; disease severity; epithelium; genes; hemolytic uremic syndrome; loci; microbiology; serotypes; virulence; Argentina; Chile; Latin America; Paraguay
Abstract:
... Shiga toxin-producing Escherichia coli (STEC) are zoonotic food pathogens associated with foodborne diarrheal illness, hemorrhagic colitis, and complications such as hemolytic uremic syndrome (HUS). The ability to adhere to epithelial cells is an important virulence trait, and pathogenicity islands (PAIs) play an important role on it. Some STEC carrying a PAI named locus of enterocyte effacement ( ...
DOI:
10.1007/s00284-020-02062-8

36. Effects of Beef Juice on Biofilm Formation by Shiga Toxin–Producing Escherichia coli on Stainless Steel

Author:
Zhi Ma; Kim Stanford; Xiao M. Bie; Yan D. Niu; Tim A. McAllister
Source:
Foodborne pathogens & disease 2020 v.17 no.4 pp. 235-242
ISSN:
1556-7125
Subject:
Shiga toxin-producing Escherichia coli; bacterial contamination; beef; biofilm; cleaning; electron microscopy; extracellular matrix; food contact surfaces; gentian violet; meat juices; meat processing; meat production; model food systems; raw meat; staining; stainless steel
Abstract:
... Shiga toxin–producing Escherichia coli (STEC) are a leading cause of foodborne illnesses worldwide, with beef and beef products as a common food reservoir. STEC strains may be present in beef-processing environments in the form of biofilms. The exudate of raw beef, also referred to as beef juice, has been identified as an important source of bacterial contamination on food-processing surfaces. Thi ...
DOI:
10.1089/fpd.2019.2716

37. Enumeration of Escherichia coli and determination of Salmonella spp. and verotoxigenic Escherichia coli in shellfish (Mytilus galloprovincialis and Ruditapes decussatus) harvested in Sardinia, Italy

Author:
Sonia Lamon; Francesca Piras; Domenico Meloni; Vanessa Agus; Gabriella Porcheddu; Margherita Pes; Maria Giovanna Cambula; Giuseppe Esposito; Federica Fois; Simonetta Gianna Consolati; Anna Mureddu
Source:
Italian journal of food safety 2020 v.9 no.4 pp. 8625
ISSN:
2239-7132
Subject:
European Union; Mytilus galloprovincialis; Ruditapes decussatus; Salmonella; Shiga toxin-producing Escherichia coli; depuration; food safety; human diseases; most probable number technique; polymerase chain reaction; risk; shellfish; Italy; Sardinia
Abstract:
... The aim of the present study was to evaluate the occurrence of Salmonella spp., Verotoxigenic E. Coli (VTEC) and enumerate E. coli in shellfish (Mytilus galloprovincialis and Ruditapes decussatus) collected before and after depuration from two class B harvesting areas located in Sardinia (Italy). All the samples were analyzed for Salmonella spp. detection according to European Commission Regulatio ...
DOI:
10.4081/ijfs.2020.8625

38. Escherichia coli strains producing a novel Shiga toxin 2 subtype circulate in China

Author:
Yang Xi; Bai Xiangning; Zhang Ji; Sun Hui; Fu Shanshan; Fan Ruyue; He Xiaohua; Scheutz Flemming; Matussek Andreas; Xiong Yanwen
Source:
International journal of medical microbiology 2020 v.310 no.1 pp. 151377
ISSN:
1438-4221
Subject:
Escherichia infections; Shiga toxin-producing Escherichia coli; Shiga-like toxin 2; bacterial contamination; coliphages; cytotoxicity; enterotoxigenic Escherichia coli; food contamination; genetic heterogeneity; genetic profile; genomics; nucleotide sequences; raw meat; serotypes; virulence; China
Abstract:
... Shiga toxin (Stx) is the key virulent factor in Shiga toxin-producing Escherichia coli (STEC), which can cause diarrhea and hemorrhagic colitis with life-threatening complications. Here, we report a novel Stx2 subtype, designated Stx2k, in E. coli strains widely detected from diarrheal patients, animals, and raw meats in China. Stx2k exhibits varied cytotoxicity in vitro among strains. The Stx2k-c ...
Handle:
10113/7191306
DOI:
10.1016/j.ijmm.2019.151377

39. Escherichia coli: Physiological Clues Which Turn On the Synthesis of Antimicrobial Molecules

Author:
Sarah-Jo Paquette; Tim Reuter
Source:
Veterinary sciences 2020 v.7 no.4 pp. -
ISSN:
2306-7381
Subject:
Shiga toxin-producing Escherichia coli; anti-infective agents; bile salts; carbon; digestive tract; experimental design; food safety; risk; salt concentration; virulence
Abstract:
... Zoonotic pathogens, like Shiga toxin-producing Escherichia coli (STEC) are a food safety and health risk. To battle the increasing emergence of virulent microbes, novel mitigation strategies are needed. One strategy being considered to combat pathogens is antimicrobial compounds produced by microbes, coined microcins. However, effectors for microcin production are poorly understood, particularly i ...
DOI:
10.3390/vetsci7040184

40. Evaluating the risks associated with Shiga-toxin-producing Escherichia coli (STEC) in private well waters in Canada

Author:
Colin Reynolds; Sylvia Checkley; Linda Chui; Simon Otto; Norman F. Neumann
Source:
Canadian journal of microbiology 2020 v.66 no.5 pp. 337-350
ISSN:
1480-3275
Subject:
Shiga toxin-producing Escherichia coli; disease outbreaks; drinking water; environmental factors; groundwater contamination; microorganisms; risk; water quality; well water; wells; Canada
Abstract:
... Shiga-toxin-producing Escherichia coli (STEC) represent a major concern for waterborne disease outbreaks associated with consumption of contaminated groundwater. Over 4 million people rely on private groundwater systems as their primary drinking water source in Canada; many of these systems do not meet current standards for water quality. This manuscript provides a scoping overview of studies exam ...
DOI:
10.1139/cjm-2019-0329

41. Farm animal contact is associated with progression to Hemolytic uremic syndrome in patients with Shiga toxin-producing Escherichia coli — Indiana, 2012–2018

Author:
Madhura S. Vachon; Myda Khalid; Gillian A.M. Tarr; Craig Hedberg; Jennifer A. Brown
Source:
One health 2020 v.11 pp. 100175
ISSN:
2352-7714
Subject:
Shiga toxin-producing Escherichia coli; direct contact; farmed animal species; farms; genes; hemolytic uremic syndrome; regression analysis; risk factors; virulence; Indiana
Abstract:
... Hemolytic uremic syndrome (HUS) is a life-threatening complication of Shiga toxin-producing Escherichia coli (STEC) infection. The relationship between STEC exposure and severity of clinical outcomes is not well documented. We examined whether direct contact with farm animals increased the likelihood of HUS among Indiana residents diagnosed with STEC.Exposure data for laboratory-confirmed STEC cas ...
DOI:
10.1016/j.onehlt.2020.100175

42. Fast, sensitive, and reliable detection of waterborne pathogens by digital PCR after coagulation and foam concentration

Author:
Atsushi Jikumaru; Satoshi Ishii; Tomoko Fukudome; Yasuhiko Kawahara; Atsushi Iguchi; Yoshifumi Masago; Kei Nukazawa; Yoshihiro Suzuki
Source:
Journal of bioscience and bioengineering 2020 v.130 no.1 pp. 76-81
ISSN:
1389-1723
Subject:
DNA; Shiga toxin; Shiga toxin-producing Escherichia coli; coagulation; disease outbreaks; foams; genes; pathogens; quantitative polymerase chain reaction; river water
Abstract:
... The quantification of pathogens is important for assessing water safety and preventing disease outbreaks. Culture-independent approaches, such as quantitative PCR (qPCR) and digital PCR (dPCR), are useful techniques for quantifying pathogens in water samples. However, since pathogens are usually present at low concentrations in water, it is necessary to concentrate microbial cells before extractin ...
DOI:
10.1016/j.jbiosc.2020.02.004

43. Gaseous antimicrobial treatments to control foodborne pathogens on almond kernels and whole black peppercorns

Author:
Bhargavi Rane; David F. Bridges; Vivian C.H. Wu
Source:
Food microbiology 2020 v.92 pp. 103576
ISSN:
0740-0020
Subject:
Listeria monocytogenes; Salmonella enterica; Shiga toxin-producing Escherichia coli; almonds; food microbiology; heat; relative humidity; serotypes
Abstract:
... Gaseous treatments with ClO₂ and O₃ on low-moisture foods (LMFs) have been reported for their efficient bacterial reduction without affecting the external quality of food. However, these studies were conducted on a small scale, which limits their application to LMF industries. We aimed to evaluate the effectiveness of gaseous antimicrobial intervention with ClO₂ or O₃ to reduce foodborne pathogens ...
DOI:
10.1016/j.fm.2020.103576

44. Genomic Insight into Natural Inactivation of Shiga Toxin 2 Production in an Environmental Escherichia coli Strain Producing Shiga Toxin 1

Author:
Michelle Qiu Carter; Antares Pham; Xiaohua He; Robert Hnasko
Source:
Foodborne pathogens & disease 2020 v.17 no.9 pp. 555-567
ISSN:
1556-7125
Subject:
Shiga toxin; Shiga toxin-producing Escherichia coli; Shiga-like toxin 1; Shiga-like toxin 2; binding sites; cattle; chromosomes; enrofloxacin; feces; food animals; genes; genomics; mitomycin; multilocus sequence typing; mutation; pathogenicity; phylogeny; plasmids; risk assessment; serotypes; tetracycline; California
Abstract:
... Shiga toxin-producing Escherichia coli (STEC) consists of a group of diverse strains differing greatly in genetic make-up and pathogenicity potential. Here, we investigated production of Shiga toxins (Stxs) in a bovine isolate carrying multiple Shiga toxin genes (stxs) after exposure to several antibiotics commonly used in food animals. Strain RM10809-C3 was co-isolated with a STEC O145:H28 strain ...
DOI:
10.1089/fpd.2019.2767

45. Global and Regional High-Resolution VTEC Modelling Using a Two-Step B-Spline Approach

Author:
Andreas Goss; Michael Schmidt; Eren Erdogan; Florian Seitz
Source:
Remote Sensing 2020 v.12 no.7 pp. -
ISSN:
2072-4292
Subject:
Shiga toxin-producing Escherichia coli; geographical distribution; global positioning systems; latitude; longitude; models; precision agriculture; remote sensing; spectral analysis; storms; Belgium; Spain; Switzerland
Abstract:
... The ionosphere is one of the largest error sources in GNSS (Global Navigation Satellite Systems) applications and can cause up to several meters of error in positioning. Especially for single-frequency users, who cannot correct the ionospheric delay, the information about the state of the ionosphere is mandatory. Dual- and multi-frequency GNSS users, on the other hand, can correct the ionospheric ...
DOI:
10.3390/rs12071198

46. Heat resistance of Shiga toxin-producing Escherichia coli and potential surrogates in wheat flour at two moisture levels

Author:
Hossein Daryaei; Qian Sui; Hui Liu; André Rehkopf; Walter Peñaloza; Andreas Rytz; Yuqi Luo; Jason Wan
Source:
Food control 2020 v.108 pp. -
ISSN:
0956-7135
Subject:
Bifidobacterium animalis subsp. lactis; Enterococcus faecium; Lactobacillus plantarum; Listeria innocua; Shiga toxin-producing Escherichia coli; apple juice; bacteria; cookies; dough; fast foods; hazelnuts; heat inactivation; heat tolerance; heat treatment; plate count; sausages; water activity; water content; wheat flour
Abstract:
... Twenty-two Shiga toxin-producing Escherichia coli (STEC) strains and six potential surrogates (E. coli strains K12 and P1, Listeria innocua, Lactobacillus plantarum, Bifidobacterium lactis, and Enterococcus faecium) were screened in wheat flour at two water content levels of 8 and 13% (0.25 and 0.55 water activities) for survival at 6 °C and resistance to a heat treatment at 82 °C for 5 min. The r ...
DOI:
10.1016/j.foodcont.2019.106788

47. Hemolysin-Producing Strains among Diarrheagenic Escherichia coli Isolated from Children under 2 Years Old with Diarrheal Disease

Author:
Anca Mare; Adrian Man; Felicia Toma; Cristina Nicoleta Ciurea; Răzvan Lucian Coșeriu; Camelia Vintilă; Adrian Cornel Maier
Source:
Pathogens 2020 v.9 no.12 pp. -
ISSN:
2076-0817
Subject:
O-antigens; Shiga toxin-producing Escherichia coli; agglutination; antibiotic resistance; bacteriology; children; diarrhea; false positive results; genes; hemolysis; hospitals; laboratories; multiple drug resistance; pathogens; serotypes
Abstract:
... Even if serotyping based on O antigens is still routinely used by most laboratories for the detection of diarrheagenic Escherichia coli, this method can provide false-positive reactions, due to the high diversity of O antigens. Molecular methods represent a valuable tool that clarifies these situations. In the Bacteriology Laboratory of Mureș County Hospital, between May 2016 and July 2019, 160 di ...
DOI:
10.3390/pathogens9121022

48. Identification and molecular characteristics of verotoxin-producing Escherichia coli (VTEC) from bovine and pig carcasses isolated in Poland during 2014–2018

Author:
Kinga Wieczorek; Jacek Osek
Source:
Food microbiology 2020 v.92 pp. 103587
ISSN:
0740-0020
Subject:
Shiga toxin-producing Escherichia coli; cattle; food microbiology; genes; humans; intimin; quantitative polymerase chain reaction; serotypes; swine; Poland
Abstract:
... The presence of verotoxin-producing Escherichia coli (VTEC) on bovine (n = 330) and pig (n = 120) carcasses in Poland was investigated using the ISO/TS 13136 standard. A total of 115 (34.8%) and 37 (30.8%) cattle and pig samples were positive in real-time PCR, respectively. Isolation of the bacteria revealed that from bovine carcasses 37 (32.2%) VTEC were obtained whereas only 5 (13.5%) pig carcas ...
DOI:
10.1016/j.fm.2020.103587

49. In Situ Generation of Chlorine Dioxide for Decontamination of Salmonella, Listeria monocytogenes, and Pathogenic Escherichia coli on Cantaloupes, Mung Beans, and Alfalfa Seeds

Author:
Jing Ni Tan; Cheng-An Hwang; Lihan Huang; Vivian C. H. Wu; Hsin-I. Hsiao
Source:
Journal of food protection 2020 v.83 no.2 pp. 287-294
ISSN:
0362-028X
Subject:
Listeria monocytogenes; Salmonella; Shiga toxin-producing Escherichia coli; alfalfa; cantaloupes; chlorine dioxide; chlorites; color; decontamination; food safety; germination; hydrochloric acid; pathogens
Abstract:
... In situ generation of chlorine dioxide to reduce microbial populations on produce surfaces has been shown to be effective on produce models. This study examined the treatment for decontamination of bacterial pathogens on whole cantaloupes and sprout seeds. Whole cantaloupes, mung beans, and alfalfa seeds were inoculated with Salmonella, Listeria monocytogenes, and Shiga toxin–producing Escherichia ...
DOI:
0362-028X.JFP-19-434

50. Inactivation of Salmonella and Shiga toxin-producing Escherichia coli (STEC) from the surface of alfalfa seeds and sprouts by combined antimicrobial treatments using ozone and electrolyzed water

Author:
Zahra Mohammad; Ahmed Kalbasi-Ashtari; Gerald Riskowski; Vijay Juneja; Alejandro Castillo
Source:
Food research international 2020 v.136 pp. 109488
ISSN:
0963-9969
Subject:
Salmonella; Shiga toxin-producing Escherichia coli; alfalfa; color; disinfection; electrolyzed water; food research; oxygen; ozonation; ozone; pH; shelf life
Abstract:
... Individual chemical and non-chemical treatments have failed to disinfect alfalfa seeds and sprouts from pathogens thoroughly. This study investigated the disinfection of alfalfa seeds and sprouts using a procedure combining ozone with acidic (pH 3.0) electrolyzed water (AEW). Inoculated alfalfa seeds with a cocktail of 3 strains Salmonella and 3 strains of STEC were treated sequentially with aqueo ...
DOI:
10.1016/j.foodres.2020.109488