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You searched for: Author "Rode, Kristina" Remove constraint Author: "Rode, Kristina" Publication Year 2020 Remove constraint Publication Year: 2020 Subject Western blotting Remove constraint Subject: Western blotting
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1. Establishment and functional characterization of a murine primary Sertoli cell line deficient of connexin43

Author:
Rode, Kristina,   et al.   ; Gerber, Jonathan; Hambruch, Nina; Langeheine, Marion; Schnepel, Nadine; Brehm, Ralph;     Show all 6 Authors
Source:
Cell and tissue research 2020 v.381 no.2 pp. 309-326
ISSN:
0302-766X
Subject:
Sertoli cells; Western blotting; assays; cell culture; cell lines; cell proliferation; dynamics; electrical resistance; fluorescent antibody technique; gap junctions; genotype; immunohistochemistry; mice; polymerase chain reaction; research; seminiferous epithelium; spermatogenesis; tight junctions
Abstract:
... The Sertoli cell (SC) specific connexin43 (Cx43) knockout (SCCx43KO) mouse line is ideal to gain insight into the mechanistic gap junction formation in SC and the seminiferous epithelium. A method for developing primary SC cultures from these mice was established, validated and successfully characterized via polymerase chain reaction, immunohistochemistry, immunofluorescence (IF), and Western blot ...
DOI:
10.1007/s00441-020-03203-y

2. Expression of claudin-11 in canine prepubertal testes, and in canine adult testes showing normal spermatogenesis, impaired spermatogenesis, or testicular neoplasia

Author:
Rode, Kristina,   et al.   ; Pörtner, Carolin; Hollenbach, Julia; Thiemeyer, Heike; Beineke, Andreas; Günzel-Apel, Anne-Rose; Brehm, Ralph;     Show all 7 Authors
Source:
Theriogenology 2020 v.148 pp. 122-131
ISSN:
0093-691X
Subject:
Leydig cells; Sertoli cells; Western blotting; adults; antibodies; atrophy; cytoplasm; dogs; fluorescent antibody technique; gene expression regulation; immunohistochemistry; messenger RNA; neoplasm progression; neoplasms; protein content; proteins; puberty; reverse transcriptase polymerase chain reaction; spermatogenesis; staining; tight junctions; transcription (genetics)
Abstract:
... The blood-testis barrier (BTB) consists of different cell-to-cell connections, including tight junction proteins like claudin-11 (CLDN11). For dogs, only limited data is published dealing with these proteins in general. Therefore, their physiological relevance, their postnatal expression, and their distribution pattern in pathological conditions, e.g. in altered spermatogenesis and testicular neop ...
DOI:
10.1016/j.theriogenology.2020.03.001