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... The present study was carried out to isolate mesenchymal stem cells (MSCs) from adipose tissue of cattle (Bos indicus), characterise them, and apply them for the treatment of mastitis and metritis in the cow. Cattle MSCs were isolated from adipose tissue near the loin region of cow. Isolated adipose tissue was subjected to enzymatic digestion using 2% collagenase with agitation at regular interval ...
DNA; antibiotics; aptasensors; food quality; human health
Abstract:
... Developing convenient and sensitive detection methods for antibiotic residues in food is beneficial for ensuring food quality and human health. The tough challenges that limit the development of sensitive, quantitative, portable, on-the-spot antibiotic detectors are the lack of simple and effective target recognition and signal amplification strategies, and direct digital quantification. Herein, w ...
... An emerging need for new classes of antibiotics is, on the one hand, evident as antimicrobial resistance continues to rise. On the other hand, the awareness of the pros and cons of chemically synthesized compounds’ extensive use leads to a search for new metabolites in already known reservoirs. Previous research showed that Paenibacillus strain (P. alvei MP1) recovered from a b ...
James K. Martin; Joseph P. Sheehan; Benjamin P. Bratton; Gabriel M. Moore; André Mateus; Sophia Hsin-Jung Li; Hahn Kim; Joshua D. Rabinowitz; Athanasios Typas; Mikhail M. Savitski; Maxwell Z. Wilson; Zemer Gitai
... The rise of antibiotic resistance and declining discovery of new antibiotics has created a global health crisis. Of particular concern, no new antibiotic classes have been approved for treating Gram-negative pathogens in decades. Here, we characterize a compound, SCH-79797, that kills both Gram-negative and Gram-positive bacteria through a unique dual-targeting mechanism of action (MoA) with undet ...
European Union; adsorption; animal-based foods; antibiotics; antibodies; biosensors; chicken meat; chickens; cost effectiveness; detection limit; enrofloxacin; fluorescence; food safety; food supply chain; human health; maximum residue limits; rapid methods; raw chicken meat; wavelengths; China
Abstract:
... HighlightsA nanobiosensor was developed for rapid detection of enrofloxacin residues in chicken meat.5-Sulfosalicylic acid was adopted in a facile method for pretreatment of chicken meat samples.The detection limit of 14.1 µg kg-1 was below the maximum residue limit for chicken meat.The total detection time from sample pretreatment to result report was less than 1.5 h.Abstract. Antibiotic residues ...
... Siderophores produced in soil by plant growth-promoting rhizobacteria (PGPRs) play several roles, including nutrient mobilizers and can be useful as plants defense elicitors. We investigated the role of a synthetic mixed ligand bis-catechol-mono-hydroxamate siderophore (SID) that mimics the chemical structure of a natural siderophore, fimsbactin, produced by Acinetobacter spp. in the resistance ag ...
antibiotic resistance; antibiotics; attitudes and opinions; bacterial infections; cats; decision making; diagnostic techniques; drug therapy; education; guidelines; medicine; pet ownership; social networks; surveys; veterinarians; veterinary clinics; veterinary drugs; United Kingdom
Abstract:
... The aim of this study was to explore owners’ knowledge of antibiotics, experience of antibiotic use in their cats and involvement in decision-making regarding antibiotic prescriptions. Cat owners were recruited via social media and veterinary practices to complete a survey designed to evaluate general knowledge of antibiotics, attitudes to antibiotic stewardship and experiences of antibiotic use i ...
... YajQ, a binding protein of the universal bacterial second messenger cyclic di‐GMP (c‐di‐GMP), affects virulence in several bacterial pathogens, including Xanthomonas campestris. In this bacterium, YajQ interacts with the transcription factor LysR. Upon c‐di‐GMP binding, the whole c‐di‐GMP‐YajQ‐LysR complex is found to dissociate from DNA, resulting in virulence gene regulation. Here, we identify a ...
... Salmonella is a foodborne pathogen constantly threating public health. The widespread use of antibiotics and globalization of the food industry result in rapid growth of drug-resistance. Eggs contaminated by multidrug-resistant (MDR) Salmonella are one of the riskiest factors of salmonellosis, which are frequently associated with outbreaks worldwide. Thus, there are increasing needs for the develo ...
Fourier transform infrared spectroscopy; antibiotics; atomic force microscopy; biomimetics; chemical composition; chitosan; ciprofloxacin; detection limit; drug formulations; electrochemistry; glassy carbon electrode; milk; mineral water; molecular imprinting; nanogold; norfloxacin; ofloxacin; polymers; roughness; tap water; voltammetry
Abstract:
... In this work, we present a novel study on the development of an electrochemical biomimetic sensor to detect the ciprofloxacin (CIP) antibiotic. A chitosan gold nanoparticles decorated molecularly imprinted polymer (Ch-AuMIP) was used to modify the glassy carbon electrode (GCE) for preparation of the sensor. The Ch-AuMIP was characterized to understand various properties like chemical composition, ...
... Following the occurrence of sudden death cases in a zoo reptile collection, histological analyses conducted on tissues from two common adders suggested an infection due to Chlamydia. The survey was extended to 22 individual snakes from the same collection and a PCR analysis targeting a conserved gene in Chlamydiaceae revealed bacterial shedding in six of them. The infection resolved spontaneously ...
... The genus Ziziphora belongs to Lamiaceae family being known as an aromatic medicinal plant. The Lamiaceae family has several species which are of great importance in pharmaceutical, food and cosmetic industry, as well as in traditional and modern medicine. In this manuscript we analyzed the chemical composition of the essential oils (EOs) isolated from the aerial flowering organs of cultivated Ziz ...
... The present study was conducted to prepare a compound plant extract as a candidate animal feed additive. Firstly, Evodia rutaecarpa (ER), Schisandra sphenanthera (SS), Punica granatum (PG) and Artemisia argyi (AA) were screened out from 17 plants as materials of candidate compound plant extracts by measuring the antibacterial rate on Escherichia coli and Salmonella paratyphoid, and the scavenging ...
animal health; antibiotic resistance; antibiotic resistance genes; antibiotics; biotechnology; feeds; information management; metadata; pathogens; pork industry; swine; swine production
Abstract:
... New Canadian regulations have required that all use of antibiotics in livestock animal production should be under veterinary prescription and oversight, while the prophylactic use and inclusion of these agents in animal feed as growth promoters are also banned. In response to this new rule, many Canadian animal producers have voluntarily implemented production practices aimed at producing animals ...
... Valnemulin (VAL) and tiamulin (TIA) are pleuromutilin antibiotics used primarily for treating bacterial infections in swine or other food animals. Furthermore, VAL and TIA are also employed as feed additives to promote animal growth. However, the illegal use of VAL and TIA could cause a series of hazards to consumers. Here, VAL was designed to be conjugated with bovine serum protein to prepare imm ...
... Lactic acid bacteria (LAB) are commonly used in soymilk fermentation to improve health-related functionality, but their contribution to sensory qualities is less valued. We characterized Lactobacillus harbinensis M1, Lactobacillus mucosae M2, Lactobacillus fermentum M4, Lactobacillus casei M8 and Lactobacillus rhamnosus C1 from naturally-fermented tofu whey, along with Streptococcus thermophilus S ...
... Marine mollusk aquaculture has more than doubled over the past twenty years, accounting for over 15% of total aquaculture production in 2016. Infectious disease is one of the main limiting factors to the development of mollusk aquaculture, and the difficulties inherent to combating pathogens through antibiotic therapies or disinfection have led to extensive research on host defense mechanisms and ...
antibiotic resistance; antibiotic resistance genes; antibiotics; bacteria; chemical compounds; constructed wetlands; ecosystems; environmental impact; flame retardants; hormones; humans; personal care products; pesticides; plasticizers; surfactants; wastewater; wastewater treatment
Abstract:
... Emerging organic contaminants (EOCs) include a diverse group of chemical compounds, such as pharmaceuticals and personal care products (PPCPs), pesticides, hormones, surfactants, flame retardants and plasticizers. Many of these compounds are not significantly removed in conventional wastewater treatment plants and are discharged to the environment, presenting an increasing threat to both humans an ...
... A robust hafnium-based metal organic framework, Hf-PBTA, with sensitive and self-calibrating dual-emissive fluorescence response towards sulfite and sulfonic derivatives, including antibiotic sulfamethazine, has been developed, which shows fast detection of sulfite ions at a concentration as low as 76 ppb. The opposite response tendency from two radiative pathways towards aromatic sulfonic molecul ...
... The adsorption with highly porous adsorbents is an efficient technique to trap the uncontrolled release of antibiotics in the environment, however, mere adsorption does not mineralize the discharged antibiotics. On the contrary, the regular photocatalysts completely mineralize the antibiotics, however suffers from high efficiency due to comparatively low surface area and porosity. In this work, a ...
Bryce P. Johnson; Erin M. Scull; Dustin A. Dimas; Tejaswi Bavineni; Chandrasekhar Bandari; Andrea L. Batchev; Eric D. Gardner; Susan L. Nimmo; Shanteri Singh
... Aromatic prenyltransferases are known for their extensive promiscuity toward aromatic acceptor substrates and their ability to form various carbon-carbon and carbon-heteroatom bonds. Of particular interest among the prenyltransferases is NphB, whose ability to geranylate cannabinoid precursors has been utilized in several in vivo and in vitro systems. It has therefore been established that prenylt ...
... Pseudomonas cannabina pv. alisalensis (Pcal), which causes bacterial blight of brassicaceous plants, is an economically important pathogen worldwide. Copper fungicide and antibiotics are major strategies to manage the disease caused by Pcal; however, a Pcal strain resistant to these chemicals has already been found, and severe outbreaks of bacterial blight have been reported on cabbage in Japan. T ...
... The antifungal echinocandin lipopeptide, acrophiarin, was circumscribed in a patent in 1979. We confirmed that the producing strain NRRL 8095 is Penicillium arenicola and other strains of P. arenicola produced acrophiarin and acrophiarin analogues. Genome sequencing of NRRL 8095 identified the acrophiarin gene cluster. Penicillium arenicola and echinocandin‐producing Aspergillus species belong to ...
... BACKGROUND: Antibiotics such as trimethoprim (TMP) are used in large quantities and recent studies show that TMP is not eliminated completely in wastewater from municipal treatment plants. Advanced oxidation processes (AOPs), especially sulfate radical‐based AOPs, are highly reactive in the presence of a catalyst. Biochar, a green and low‐cost carbon material, is an excellent catalyst to degrade p ...
... An active packaging film based on low‐density polyethylene (LDPE)/ethylene‐vinyl acetate (EVA) copolymer was prepared. Oregano essential oil microcapsules were prepared by spray drying and added as active antibacterial ingredients to the LDPE/EVA film by melt blending and extrusion casting and enhanced the moisture permeability and gas permeability of the film. The active packaging film effectivel ...
... Fluoroquinolones (FQs) present in water environments pose threats to aquatic organisms. The concentration of FQs adsorbed onto sediments is generally higher than that in water. Here, we studied the toxicities of two FQ antibiotics, norfloxacin (NOR) and ofloxacin (OFL), on the snail Bellamya aeruginosa, an adaptable benthic animal. For this, we performed acute toxicity experiments on young snails ...
Streptomyces; antibiotics; bacteria; biosynthesis; chemical reactions; chemical structure; coenzyme A; cysteamine; drugs; enzymes; inhibitory concentration 50; virulent strains
Abstract:
... Phosphopantetheine is a key structural element in biological acyl transfer reactions found embedded within coenzyme A (CoA). Phosphopantothenoylcysteine synthetase (PPCS) is responsible for installing a cysteamine group within phosphopantetheine. Therefore, it holds considerable potential as a drug target for developing new antimicrobials. In this study, we adapted a biochemical assay specific for ...
... The antibiotic doxorubicin is often used as an anti-neoplastic drug; however, many patients showed very unpleasant side-effects. Previous studies have demonstrated that dietary substances such as Aloe arborescens, Annona muricata, Morinda citrifolia, Beta rubra, Scutellaria baicalensis, and Vaccinium myrtillus may have anti-oxidant, anti-proliferative, and anti-inflammatory effects. The purpose of ...
... Due to regulation and consumer-driven decreases in the use of antibiotics, there is a critical need for the development of alternatives to antibiotic growth promoters (AGP) for use in poultry production. Direct-fed microorganisms (DFM) and prebiotics, which have been demonstrated to improve growth performance and microbial safety of poultry, are thought to be potentially important alternatives to ...
... The present study aims to investigate the adsorption of synthesized poly(2-acrylamide-2-methylpropane sulfonic acid) (PAMPs) onto alumina nanoparticles and their application in the removal of ciprofloxacin (CFX) antibiotic from a water environment. The PAMPs were successfully synthesized and characterized by nuclear magnetic resonance and gel-permeation chromatography methods. The number- and weig ...
... Adsorption and desorption processes were studied for the antibiotics sulfamethoxypyridazine (SMP) and enrofloxacin (ENR) in 20 agricultural soils devoted to wheat-potato and vine cultivation. Batch experiments were used to conduct kinetic studies, as well as to evaluate adsorption and desorption for different concentrations of antibiotics. The results indicated that adsorption curves were linear f ...
... This study investigates the adsorption behavior of Ciprofloxacin (CPX) antibiotic on polyethylene (PE) microplastics through batch adsorption experiments under various environmental conditions, i.e., ionic strengths, pH and in the presence of dissolved organic matter (DOM). The adsorption of CPX showed a gradual increase with increasing pH reaching the maximum adsorption at pH 6.5–7.5 and then dec ...
Aeromonas veronii; Xiphophorus; antibacterial properties; antibiotic resistance; antibiotics; disease control; fish diseases; fish industry; gills; histology; histopathology; in vivo studies; intestines; lethal dose 50; liver; nanoparticles; nanosilver; ornamental fish; pathogens; silver; survival rate; zinc oxide
Abstract:
... Bacterial pathogens cause significant challenge to the ornamental fish industry. Eventhough antibiotic administration has been recommended to manage fish diseases, there is alarming concern with emergence of antibiotic resistance. This indicates the need for the development of alternative methods with multi-targeted action to manage fish diseases. In the study, silver (AgNPs) and zinc oxide (ZnONP ...
... Traumatic reticuloperitonitis (TRP) in cattle is caused by ingested nails, pieces of wire, and other nonmetallic materials that injure the reticular wall. Clinical signs of acute TRP may include anorexia, fever, drop in milk production, rumen atony and tympany, abdominal pain, an arched back, a tucked up and “guarded” abdomen and spontaneous grunting, but may be obscure or absent in chronic cases. ...
... In this study, agar/κ-carrageenan/montmorillonite (MMT) hydrogels were prepared to examine their usability as wound dressing materials and to see the effect of MMT amount on some properties of agar/κ-carrageenan hydrogel materials. Hydrogels were characterized by SEM-EDX, TEM and DSC analyses. By increasing the MMT content within hydrogel matrix from 0% to 5%, the decomposition temperature of the ...
... According to investigations in phytomedicine and ethnopharmacology, the therapeutic properties of garlic (Allium sativum) have been described by ancestral cultures. Notwithstanding, it is of particular concern to elucidate the molecular mechanisms underlying this millenary empirical knowledge. Allicin (S-allyl prop-2-ene-1-sulfinothioate), a thioester of sulfenic acid, is one of the main bioactive ...
... The cellular redox balance plays a significant role in cell fate decisions and in the regulation of responses to various kinds of stress. In this study, we defined a novel concept of the oxidative-redox metabolome, and established a method for the simultaneous quantification of 23 metabolites involved in the oxidative-redox metabolome, covering NAD⁺ pathway, FAD pathway, GSSG pathway, and ATP path ...
Staphylococcus aureus; alginates; alkaline phosphatase; antibiotics; biocompatibility; bone formation; bones; calcium phosphates; cement; humans; ligaments; microbeads; modulus of elasticity; modulus of rupture; orthopedics; osteomyelitis; stem cells; tissue repair
Abstract:
... Osteomyelitis and post-operative infections are major problems in orthopedic, dental and craniofacial surgeries. It is highly desirable for a tissue engineering construct to kill bacteria, while simultaneously delivering stem cells and enhancing cell function and tissue regeneration. The objectives of this study were to: (1) develop a novel injectable calcium phosphate cement (CPC) scaffold contai ...
Escherichia coli; Klebsiella; Proteus vulgaris; Pseudomonas aeruginosa; Salmonella; Serratia; Shigella dysenteriae; Staphylococcus aureus; antibiotics; boiling; chlorides; chlorination; coliform bacteria; drinking water; filtration; most probable number technique; physicochemical properties; public health; sustainable development; total dissolved solids; water hardness; water supply; well water; wells; Nigeria
Abstract:
... A total of one hundred and twenty-two water samples were obtained from hand-dug wells and boreholes in Ogbomoso metropolis, Oyo State, Nigeria. Physico-chemical, most probable number technique, direct culture methods, and water quality index were used to assess the drinking water quality in this region. Some of the physicochemical parameters were within the acceptable limits, with the exemption of ...
... Consumer demands for chickens raised without the use of antibiotics, legislative restrictions, and trade opportunities have encouraged many integrators within the poultry industry to raise poultry without antibiotic growth promoters (AGPs) and ionophores. However, with the removal of AGPs and ionophores, the incidence of enteric diseases such as coccidiosis and necrotic enteritis (NE), caused by E ...
... Pingyangmycin (PYM) and boanmycin (BAM), two individual components of bleomycin (bleomycin A5 and bleomycin A6), are glycopeptide antitumor antibiotics. An efficient procedure for the preparation of PYM and BAM from Streptomyces verticillus var. pingyangensis fermentation broth using macroporous cation-exchange (MCE) resin followed by medium-pressure preparative liquid chromatography (MPLC) based ...
... Two-component signal transduction systems (TCSs), consisting of a histidine kinase (HK) and its cognate response regulator, are ubiquitous among bacteria and are associated with the virulence of pathogens. TCSs are potential targets for alternative antibiotics and antivirulence agents. It is, thus, very important to determine HK activity in bacterial TCSs. Here, we describe an immuno-dot blot assa ...
... Pseudomonas aeruginosa is an opportunistic pathogen exhibiting higher resistance to commonly used antibiotics. Microbial resistance to antibiotics is a major problem that hinders attempts to control microbial infections. Quorum sensing inhibitors could help us solve such problem by repressing quorum sensing that controls the production of virulence factors in many pathogens including P. aeruginosa ...
antibiotic resistance; antibiotics; diagnostic techniques; landscapes; markets; public health; research and development; uncertainty
Abstract:
... Antimicrobial resistance (AMR) is an urgent public health threat, and continues to be on the rise. Basic microbiological research is the foundation for addressing knowledge gaps both for the development of new antibiotics, diagnostics and preventives but also to inform strategies to mitigate the transmission of resistance and drug resistant microorganisms. Translating this research into new produc ...
... Hemigraphis alternata (HA) known as Murikuti, is a traditionally used medicinal plant. Phytochemical screening of leaves shows the presence of bioactive reducing agents. Gold nanoparticles were prepared using aqueous extract of HA leaves at various conventional methods. Concentration variation of gold nanoparticles was studied and optimized under room temperature conditions. Gold nanoparticles (vi ...
... To aid the development of novel antibacterial agents that possess a innovative mechanism of action, we built a series of novel dithiocarbamate-containing 4H-chromen-4-one derivatives. We evaluated the activities of the derivatives against three plant pathogens Xanthomonas oryzae pv oryzae (X. oryzae pv o.), Ralstonia solanacearum (R. solanacearum), and Xanthomonas axonopodis pv citri (X. axonopodi ...
... Defensins represent an evolutionary ancient family of antimicrobial peptides, which played an undeniably important role in host defense. In the present study, a defensin isoform was identified and characterized from manila clam Ruditapes philippinarum (designed as Rpdef1α). Multiple alignments and phylogenetic analysis suggested that Rpdef1α belonged to the defensin family. Quantitative RT-PCR and ...
... Natural preservatives are substances that are secreted by organisms or present in the body and have bacteriostatic effects, so they are not toxic to humans. Natural preservatives have become a research focus because of their high safety and strong antibacterial effects. Some commodities in the market are added with tea saponins, such as a brand of foaming cleanser, shampoo, and toothpaste rich in ...
... Probiotics can stabilize gut flora, regulate intestinal immunity and protect the host from enteric diseases; however, their roles in oral health have received little attention compared to their roles in gut health. Nowadays, the prevalence of sugar‐sweetened foods and abuse of antibiotics contribute towards dysbiosis of oral microbiota and drug resistance development in oral pathogens, resulting i ...
... New antibacterial agents are urgently required to fight the emergence of antibiotic-resistant bacteria. We recently synthesized the first thioimidazolium ionene, which has antibacterial properties and can degrade in various media. This dual functionality is crucial in order to limit the environmental impact of these biocides. We have found that our polymer is stronger than benzalkonium chloride (B ...