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You searched for: Publication year rev 7980-2020 Remove constraint Publication year rev: 7980-2020 Subject term bacteria Remove constraint Subject term: bacteria
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1. 1,2H hyperfine spectroscopy and DFT modeling unveil the demethylmenasemiquinone binding mode to E. coli nitrate reductase A (NarGHI)

Author:
Maryam Seif Eddine; Frédéric Biaso; Julia Rendon; Eric Pilet; Bruno Guigliarelli; Axel Magalon; Stéphane Grimaldi
Source:
Biochimica et biophysica acta 2020 v.1861 no.8 pp. 148203
ISSN:
0005-2728
Subject:
Escherichia coli; bacteria; deuterium oxide; electron paramagnetic resonance spectroscopy; isoprenoids; isotropy; menaquinones; models; nitrate reductase; oxidation; protons; ubiquinones
Abstract:
... The quinol oxidation site QD in E. coli respiratory nitrate reductase A (EcNarGHI) reacts with the three isoprenoid quinones naturally synthesized by the bacterium, i.e. ubiquinones (UQ), menaquinones (MK) and demethylmenaquinones (DMK). The binding mode of the demethylmenasemiquinone (DMSK) intermediate to the EcNarGHI QD quinol oxidation site is analyzed in detail using ¹,²H hyperfine (hf) spect ...
DOI:
10.1016/j.bbabio.2020.148203

2. 16S rRNA Sequencing Detected Profftella, Liberibacter, Wolbachia, and Diplorickettsia from Relatives of the Asian Citrus Psyllid

Author:
Atsushi Nakabachi; Igor Malenovský; Ilia Gjonov; Yuu Hirose
Source:
Microbial ecology 2020 v.80 no.2 pp. 410-422
ISSN:
0095-3628
Subject:
Candidatus Liberibacter; Citrus; Diaphorina citri; Oceanospirillales; Psyllidae; Wolbachia; arthropods; bacteria; bacteriome; etiological agents; evolution; fruit diseases; genes; greening disease; horticulture; hosts; insects; microbiome; mutualism; pests; plants (botany); reproduction; ribosomal RNA; sequence analysis; symbionts
Abstract:
... The Asian citrus psyllid Diaphorina citri (Hemiptera: Psylloidea) is a serious pest of citrus species worldwide because it transmits Candidatus Liberibacter spp. (Alphaproteobacteria: Rhizobiales), the causative agents of the incurable citrus disease, huanglongbing or greening disease. Diaphorina citri possesses a specialized organ called a bacteriome, which harbors vertically transmitted intracel ...
DOI:
10.1007/s00248-020-01491-z

3. 16S rRNA Sequencing and Metagenomics Study of Gut Microbiota: Implications of BDB on Type 2 Diabetes Mellitus

Author:
Liang Zhang; Jiao Luo; Xiangqian Li; Shuju Guo; Dayong Shi
Source:
Marine drugs 2020 v.18 no.9 pp. -
ISSN:
1660-3397
Subject:
Bacteroides; Lachnospiraceae; Rhodomela; bacteria; blood glucose; genes; glycemic effect; intestinal microorganisms; metabolic diseases; metabolism; metagenomics; metformin; mice; research; sequence analysis; starch; sucrose
Abstract:
... Gut microbiota has a critical role in metabolic diseases, including type 2 diabetes mellitus (T2DM). 3-bromo-4,5-bis(2,3-dibromo-4,5-dihydroxybenzyl)-1,2-benzenediol (BDB) is a natural bromophenol isolated from marine red alga Rhodomela confervoides. Our latest research showed that BDB could alleviate T2DM in diabetic BKS db mice. To find out whether BDB modulates the composition of the gut microb ...
DOI:
10.3390/md18090469

4. 2,3-Dihydroxyisovalerate production by Klebsiella pneumoniae

Author:
Yike Wang; Jinjie Gu; Xiyang Lu; Zhongxi Zhang; Yang Yang; Shaoqi Sun; Emily T. Kostas; Jiping Shi; Mintian Gao; Frank Baganz; Gary J. Lye; Jian Hao
Source:
Applied microbiology and biotechnology 2020 v.104 no.15 pp. 6601-6613
ISSN:
0175-7598
Subject:
Klebsiella pneumoniae; bacteria; batch fermentation; biosynthesis; biotechnology; byproducts; carbon; culture media; genes; glucose; lactic acid; leucine; metabolites; oxygen; pH; valine
Abstract:
... 2,3-Dihydroxyisovalerate is an intermediate of valine and leucine biosynthesis pathway; however, no natural microorganism has been found yet that can accumulate this compound. Klebsiella pneumoniae is a useful bacterium that can be used as a workhorse for the production of a range of industrially desirable chemicals. Dihydroxy acid dehydratase, encoded by the ilvD gene, catalyzes the reaction of 2 ...
DOI:
10.1007/s00253-020-10711-y

5. 24-Epibrassinolide combined with heavy metal resistant bacteria enhancing phytoextraction of Amaranthus hypochondriacus L. in Cd-contaminated soil

Author:
Yanluo Xie; Yao Luo; Mingping Sheng; He Peng; Yuanming Gu; Heng Xu; Yun Zhao
Source:
Journal of hazardous materials 2020 v.399 pp. 123031
ISSN:
0304-3894
Subject:
Amaranthus hypochondriacus; Serratia; antioxidants; bacteria; beta-fructofuranosidase; bioavailability; community structure; enzyme activity; heavy metals; metal tolerance; microbial communities; phosphates; phytoaccumulation; plant growth; plant hormones; polluted soils; soil pH; urease
Abstract:
... The remediation efficiency of phytoextraction on heavy metal could be influenced by metal bioavailability and plant growth. Hence, we applied a synergistic intensification system with plant hormone (24-Epibrassinolide, EBR) and metal-resistant bacterium (Serratia sp. CTZ4) to enhance Cd extraction of Amaranthus hypochondriacus L. in contaminated soil. Results demonstrated that the combination of C ...
DOI:
10.1016/j.jhazmat.2020.123031

6. 3-D printed polyvinyl alcohol matrix for detection of airborne pathogens in respiratory bacterial infections

Author:
Alaa Al-Taie; Xiaoxiao Han; Caroline M. Williams; Mohamad Abdulwhhab; Andrew P. Abbott; Alex Goddard; Malgorzata Wegrzyn; Natalie J. Garton; Michael R. Barer; Jingzhe Pan
Source:
Microbiological research 2020 v.241 pp. 126587
ISSN:
0944-5013
Subject:
DNA; Mycobacterium abscessus; Mycobacterium bovis BCG; Mycobacterium smegmatis; aerosols; air; airborne pathogens; animals; bacteria; bacterial infections; brittleness; collagen; computer software; computer-aided design; enzymes; face masks; filters; gelatin; hydrolysis; models; morphs; polyvinyl alcohol; quantitative analysis; quantitative polymerase chain reaction; synthetic products; three-dimensional printing
Abstract:
... Novel sampling matrices were manufactured using 3D printing for the detection of respiratory pathogens in expired air. A specific configuration of the matrices was designed using Computer-Aided Design software. Polyvinyl alcohol (PVA) was printed using fused deposition modelling to create a multilayer matrix to enhance the capture of bacteria. The performance of these matrices was compared with ge ...
DOI:
10.1016/j.micres.2020.126587

7. 3-Nitrooxypropanol supplementation had little effect on fiber degradation and microbial colonization of forage particles when evaluated using the in situ ruminal incubation technique

Author:
Xiu Min Zhang; Robert J. Gruninger; Aklilu W. Alemu; Min Wang; Zhi Liang Tan; Maik Kindermann; Karen A. Beauchemin
Source:
Journal of dairy science 2020 v.103 no.10 pp. 8986-8997
ISSN:
0022-0302
Subject:
Bacteroidetes; Fibrobacteres; Firmicutes; Methanobrevibacter; bacteria; bacterial communities; bags; barley; beef cattle; cannulas; cross-over studies; dietary supplements; enzyme inhibitors; enzymes; forage; grass hay; hydrogen; methane; methane production; methanogens; microbial colonization; multidimensional scaling; neutral detergent fiber; phylogeny; rumen fluids; silage; species diversity
Abstract:
... 3-Nitrooxypropanol (3-NOP) is an investigational compound that acts as an enzyme inhibitor to decrease ruminal methanogenesis. We hypothesized that when feeding 3-NOP to cattle fed a high-forage diet, H₂ would accumulate in the rumen, which could suppress microbial colonization of feed particles and fiber degradation. Therefore, the study investigated the effects of supplementing a high-forage die ...
DOI:
10.3168/jds.2019-18077

8. 3D macroscopic graphene oxide/MXene architectures for multifunctional water purification

Author:
Xin Ming; Ankang Guo; Qian Zhang; Zhenzhen Guo; Fang Yu; Baofei Hou; Yu Wang; Kevin Peter Homewood; Xianbao Wang
Source:
Carbon 2020 v.167 pp. 285-295
ISSN:
0008-6223
Subject:
aerogels; bacteria; carbon; energy; evaporation rate; graphene oxide; irradiation; solar energy; steam; summer; water purification; winter
Abstract:
... Solar-driven interfacial evaporation is an efficient and economical technology for converting low-grade solar energy to high-grade steam energy. However, solar water evaporators that exhibit both high water vapor generation ability and multifunction water purification are still less reported. Herein, the 2D Ti₃C₂Tx (MXene) is tactfully assembled into 3D porous macroscopic aerogel monoliths by a gr ...
DOI:
10.1016/j.carbon.2020.06.023

9. Escherichia coli Strains with Virulent Factors Typical for Uropathogens were Isolated from Sinuses from Patients with Chronic Rhinosinusitis—Case Report

Author:
Beata Krawczyk; Michał Michalik; Magdalena Fordon; Magdalena Wysocka; Alfred Samet; Bogdan Nowicki
Source:
Pathogens 2020 v.9 no.5 pp. -
ISSN:
2076-0817
Subject:
Escherichia coli; bacteria; biofilm; biopsy; drug resistance; dysbiosis; enterobactin; gastrointestinal system; genes; iron; niches; pathogens; patients; phylogeny; sinuses; strains; testing; toxins; urinary tract; virulence
Abstract:
... Escherichia coli were isolated from three patients with chronic rhinosinusitis (CRS) by intraoperative sinus tissue biopsy. Taking into account the unusual replicative niche and previous treatment failures, it was decided to focus on the virulence and drug resistance of these bacteria. The strains turned out to be multi-sensitive, but the rich virulence factors profile of bacteria typical f ...
DOI:
10.3390/pathogens9050318

10. Listeria monocytogenes Response to Anaerobic Environments

Author:
Brandy N. Roberts; Damayanti Chakravarty; J.C. Gardner; Steven C. Ricke; Janet R. Donaldson
Source:
Pathogens 2020 v.9 no.3 pp. -
ISSN:
2076-0817
Subject:
Listeria monocytogenes; anaerobic conditions; bacteria; dairy products; elderly; essential genes; fetus; food industry; fruits; knowledge; listeriosis; luncheon meats; oxygen; pH; pathogens; pregnant women; ready-to-eat foods; temperature; vegetables; virulence
Abstract:
... Listeria monocytogenes is a Gram-positive facultative anaerobic bacterium that is responsible for the disease, listeriosis. It is particularly lethal in pregnant women, the fetus, the elderly and the immunocompromised. The pathogen survives and replicates over a wide range of temperatures (4 to 42 °C), pH, salt and oxygen concentrations. Because it can withstand various environments,
DOI:
10.3390/pathogens9030210

11. Magnetotactic Bacteria and Magnetosomes as Smart Drug Delivery Systems: A New Weapon on the Battlefield with Cancer?

Author:
Danuta Kuzajewska; Agata Wszołek; Wojciech Żwierełło; Lucyna Kirczuk; Agnieszka Maruszewska
Source:
Biology 2020 v.9 no.5 pp. -
ISSN:
2079-7737
Subject:
DNA; antibodies; antineoplastic agents; bacteria; crystals; drug therapy; ferromagnetism; ligands; magnetotaxis; nanocarriers; neoplasm cells; neoplasms; organelles; small interfering RNA; tissues; transporters; vaccines; water reservoirs
Abstract:
... An important direction of research in increasing the effectiveness of cancer therapies is the design of effective drug distribution systems in the body. The development of the new strategies is primarily aimed at improving the stability of the drug after administration and increasing the precision of drug delivery to the destination. Due to the characteristic features of cancer cells, distributing ...
DOI:
10.3390/biology9050102

12. Staphylococcus aureus Nasal Colonization among Children with Sickle Cell Disease at the Children’s Hospital, Accra: Prevalence, Risk Factors, and Antibiotic Resistance

Author:
Vera A. Appiah; George A. Pesewu; Fleischer C. N. Kotey; Alahaman Nana Boakye; Samuel Duodu; Edem M. A. Tette; Mame Y. Nyarko; Eric S. Donkor
Source:
Pathogens 2020 v.9 no.5 pp. -
ISSN:
2076-0817
Subject:
age; antibiotic resistance; bacteria; cefoxitin; children; clindamycin; erythromycin; gentamicin; history; hospitals; linezolid; males; methicillin-resistant Staphylococcus aureus; multiple drug resistance; nose; pathogens; penicillins; polymerase chain reaction; prevalence; questionnaires; regression analysis; residential housing; rifampicin; risk factors; sampling; sickle cell anemia
Abstract:
... The aim of this study was to investigate S. aureus carriage among children with sickle cell disease (SCD), including the prevalence, risk factors, and antibiotic resistance. The study was cross-sectional, and involved 120 children with SCD recruited at the Princess Marie Louise Children’s Hospital (PML) in Accra and 100 apparently healthy children from environs of the hospital. Nasal ...
DOI:
10.3390/pathogens9050329

13. A Biochemical Lanthanide-Encoding Approach Enables Quantitative Monitoring of the Bacterial Response to Vancomycin Treatment

Author:
Weitong Zhao; Yong Liang; Xiaowen Yan; Limin Yang; Qiuquan Wang
Source:
Biochemistry 2020 v.59 no.38 pp. 3523-3528
ISSN:
1520-4995
Subject:
Staphylococcus aureus; accuracy; amino acids; antibiotic resistance; atomic absorption spectrometry; bacteria; cell walls; image analysis; information; isotope dilution technique; liquids; monitoring; peptidoglycans; pretreatment; resistance mechanisms; vancomycin
Abstract:
... A pathogenic bacterium has its own mechanisms for not only pathogenic attack but also exogenous invasion defense, in which the bacterial cell wall is the front line of attack and defense. We developed a biochemical lanthanide-encoding approach to quantify the uncanonical d-amino acid (d-X) that was edited in a small proportion into the terminal acyl-d-Ala-d-X of nascent peptidoglycan UDP-MurNAc-pe ...
DOI:
10.1021/acs.biochem.0c00614

14. A Biocontrol Strain of Pseudomonas aeruginosa CQ-40 Promote Growth and Control Botrytis cinerea in Tomato

Author:
Xingyuan Wang; Xinan Zhou; Zhibo Cai; Lan Guo; Xiuling Chen; Xu Chen; Jiayin Liu; Mingfang Feng; Youwen Qiu; Yao Zhang; Aoxue Wang
Source:
Pathogens 2020 v.10 no.1 pp. -
ISSN:
2076-0817
Subject:
Botrytis cinerea; Pseudomonas aeruginosa; antibacterial properties; bacteria; biological control; catechol oxidase; chitinase; disease resistance; endo-1,4-beta-glucanase; fruit quality; fruits; gray mold; growth promotion; hydrogen cyanide; metabolites; nitrogen; nucleotide sequences; nutritive value; peroxidase; phenylalanine ammonia-lyase; phosphorus; plant height; proteinases; rhizosphere; seed germination; seedling growth; sequence analysis; soil; superoxide dismutase; tomatoes
Abstract:
... Botrytis cinerea infection can be very devastating for tomato production, as it can result in a large-scale reduction in tomato fruit production and fruit quality after harvest. Thus, it negatively affects tomato yield and quality. In this study, a biocontrol bacteria CQ-4 was isolated and screened from the rhizosphere soil of tomato plants. Morphological, physiological, and biochemical characteri ...
DOI:
10.3390/pathogens10010022

15. A Bi₂S₃@mSiO₂@Ag nanocomposite for enhanced CT visualization and antibacterial response in the gastrointestinal tract

Author:
Yan Zu; Liang Yan; Tao Wang; Dongqing Ma; Xinghua Dong; Zhen Du; Wenyan Yin
Source:
Journal of materials chemistry B 2020 v.8 no.4 pp. 666-676
ISSN:
2050-7518
Subject:
animal disease models; antibacterial properties; antibiotics; bacteria; biomedical research; chemistry; computed tomography; cytotoxicity; enteritis; gastrointestinal system; hemolysis; image analysis; multiple drug resistance; nanocomposites; nanosilver; oral administration; silica; sulfides
Abstract:
... The non-invasive imaging of the gastrointestinal (GI) tract is highly desired for clinical research due to the various GI tract bacterial infection-induced diseases. To treat GI tract infections, various antibiotics have been used in the clinic. The growing problem of multidrug-resistant bacteria calls for effective antibiotic alternatives. Here, we construct a dual-functional Bi₂S₃@mSiO₂@Ag nanoc ...
DOI:
10.1039/c9tb02562f

16. A Classical Molecular Dynamics Simulation Study of Interfacial and Bulk Solution Aggregation Properties of Dirhamnolipids

Author:
Charles M. Luft; Elango Munusamy; Jeanne E. Pemberton; Steven D. Schwartz
Source:
Journal of physical chemistry 2020 v.124 no.5 pp. 814-827
ISSN:
1520-5207
Subject:
Pseudomonas aeruginosa; bacteria; biodegradability; biosurfactants; hydrophilicity; hydrophobicity; liquid-air interface; molecular dynamics; molecular weight; rhamnolipids; rhamnose; surface area
Abstract:
... The rhamnolipids are a unique class of biosurfactants produced by the bacteria Pseudomonas aeruginosa. These molecules display a high level of surface activity as well as biodegradability. In this study nonionic dirhamnolipid was investigated by utilizing molecular dynamics simulation at the air–water interface as well as in bulk water. Detailed structural analysis is presented for both the interf ...
DOI:
10.1021/acs.jpcb.9b08800
PubMed:
31958226
PubMed Central:
PMC7042949

17. A Comparative Study of Organic and Conventional Management on the Rhizosphere Microbiome, Growth and Grain Quality Traits of Tritordeum

Author:
Giovanna Visioli; Marta Lauro; Teofilo Vamerali; Cristian Dal Cortivo; Anna Panozzo; Silvia Folloni; Cristina Piazza; Roberto Ranieri
Source:
Agronomy 2020 v.10 no.11 pp. -
ISSN:
2073-4395
Subject:
Bacteroidetes; Hordeum chilense; Triticum turgidum subsp. durum; administrative management; agronomy; antioxidants; bacteria; barley; breeding; calcium; comparative study; crop management; cultivars; durum wheat; glutenins; grain quality; grain yield; hexaploidy; human nutrition; iron; light; microbiome; molecular weight; nutrients; organic production; phenols; potassium; rhizosphere; root growth; sulfur; zinc
Abstract:
... Tritordeum is a novel hexaploid cereal derived from the cross between a wild Chilean barley species (Hordeum chilense Roem. et Schultz) and durum wheat (Triticum turgidum ssp. durum Desf.) that is potentially of great interest for human nutrition. In this study, a commercial and an experimental Tritordeum cultivar were analyzed in comparison with a reference durum wheat under conventional and orga ...
DOI:
10.3390/agronomy10111717

18. A Comprehensive Understanding of Electro-Fermentation

Author:
Drishti Dinesh Bhagchandanii; Rishi Pramod Babu; Jayesh M. Sonawane; Namita Khanna; Soumya Pandit; Dipak A. Jadhav; Santimoy Khilari; Ram Prasad
Source:
Fermentation 2020 v.6 no.3 pp. -
ISSN:
2311-5637
Subject:
bacteria; biochemical pathways; biomass production; carbon; design; economic sustainability; electrochemistry; electrodes; electrons; fermentation; fermenters; industrial applications; methane production; mixed culture; pH; protons; strains
Abstract:
... Electro-fermentation (EF) is an upcoming technology that can control the metabolism of exoelectrogenic bacteria (i.e., bacteria that transfer electrons using an extracellular mechanism). The fermenter consists of electrodes that act as sink and source for the production and movement of electrons and protons, thus generating electricity and producing valuable products. The conventional process of f ...
DOI:
10.3390/fermentation6030092

19. A C‐type lectin with antibacterial activity in weather loach, Misgurnus anguillicaudatus

Author:
Xiao‐Wen Zhang; Cong‐Hui Yang; Hong‐Quan Zhang; Xin‐Tong Pan; Ze‐Yu Jin; Hong‐Wei Zhang; Xiao‐Hua Xia
Source:
Journal of fish diseases 2020 v.43 no.12 pp. 1531-1539
ISSN:
0140-7775
Subject:
Misgurnus anguillicaudatus; amino acids; antibacterial properties; bacteria; bacterial growth; carbohydrate binding; hemagglutination; in vitro studies; lectins; pathogens; peptides; polysaccharides; receptors; signal peptide; weather
Abstract:
... C‐type lectins are carbohydrate‐binding proteins that play important roles in immunity by serving as pattern recognition receptors. In the present study, a novel nattectin‐like C‐type lectin was obtained from the weather loach, Misgurnus anguillicaudatus, designated as MaCTL. MaCTL encodes a peptide with 165 amino acids, with a signal peptide and a single C‐type lectin domain (CTLD), containing a ...
DOI:
10.1111/jfd.13255

20. A Deep Learning Approach to Antibiotic Discovery

Author:
Jonathan M. Stokes; Kevin Yang; Kyle Swanson; Wengong Jin; Andres Cubillos-Ruiz; Nina M. Donghia; Craig R. MacNair; Shawn French; Lindsey A. Carfrae; Zohar Bloom-Ackermann; Victoria M. Tran; Anush Chiappino-Pepe; Ahmed H. Badran; Ian W. Andrews; Emma J. Chory; George M. Church; Eric D. Brown; Tommi S. Jaakkola; Regina Barzilay; James J. Collins
Source:
Cell 2020 v.180 no.4 pp. 688-702.e13
ISSN:
0092-8674
Subject:
Acinetobacter baumannii; Clostridium difficile; Enterobacteriaceae; Mycobacterium tuberculosis; animal models; antibacterial properties; antibiotic resistance; antibiotics; bacteria; databases; drugs; pathogens; phylogeny; prediction
Abstract:
... Due to the rapid emergence of antibiotic-resistant bacteria, there is a growing need to discover new antibiotics. To address this challenge, we trained a deep neural network capable of predicting molecules with antibacterial activity. We performed predictions on multiple chemical libraries and discovered a molecule from the Drug Repurposing Hub—halicin—that is structurally divergent from conventio ...
DOI:
10.1016/j.cell.2020.01.021

21. A Drone-Based Bioaerosol Sampling System to Monitor Ice Nucleation Particles in the Lower Atmosphere

Author:
Paul Bieber; Teresa M. Seifried; Julia Burkart; Jürgen Gratzl; Anne Kasper-Giebl; David G. Schmale; Hinrich Grothe
Source:
Remote Sensing 2020 v.12 no.3 pp. -
ISSN:
2072-4292
Subject:
Betula pendula; Pinus; altitude; atmospheric pressure; bacteria; bioaerosols; climate; computers; coniferous forests; cooling; droplets; emissions; fluorescence microscopy; freezing; fungal spores; hydrologic cycle; ice nucleation; laboratory experimentation; latex; particulates; pollen; polystyrenes; relative humidity; remote sensing; temperature; terrestrial ecosystems; troposphere; Austria
Abstract:
... Terrestrial ecosystems can influence atmospheric processes by contributing a huge variety of biological aerosols (bioaerosols) to the environment. Several types of biological particles, such as pollen grains, fungal spores, and bacteria cells, trigger freezing processes in super-cooled cloud droplets, and as such can contribute to the hydrological cycle. Even though biogenic particles are known as ...
DOI:
10.3390/rs12030552

22. A Filter Supported Surface-Enhanced Raman Scattering “Nose” for Point-of-Care Monitoring of Gaseous Metabolites of Bacteria

Author:
Jingxing Guo; Ying Liu; Yuanjiao Yang; Yumei Li; Ruiyong Wang; Huangxian Ju
Source:
Analytical chemistry 2020 v.92 no.7 pp. 5055-5063
ISSN:
1520-6882
Subject:
Escherichia coli; Pseudomonas aeruginosa; Raman spectroscopy; Staphylococcus aureus; bacteria; equipment; filtration; food safety; food spoilage; gold; humans; metabolites; monitoring; nanogold; point-of-care testing; public health; rapid methods
Abstract:
... This work designs a convenient method for fabrication of surface-enhanced Raman scattering (SERS) devices by loading gold nanostars (AuNSs) on a flat filter support with vacuum filtration. The dense accumulation of AuNSs results in a strong sensitization to SERS signal and shows sensitive response to gaseous metabolites of bacteria, which produces a SERS “nose” for rapid point-of-care monitoring o ...
DOI:
10.1021/acs.analchem.9b05400

23. A Fluorogenic Trehalose Probe for Tracking Phagocytosed Mycobacterium tuberculosis

Author:
Tingting Dai; Jinghang Xie; Qihua Zhu; Mireille Kamariza; Ke Jiang; Carolyn R. Bertozzi; Jianghong Rao
Source:
Journal of the American Chemical Society 2020 v.142 no.36 pp. 15259-15264
ISSN:
1520-5126
Subject:
Mycobacterium tuberculosis; bacteria; biochemical pathways; cell walls; fluorescence; fluorescent dyes; macrophages; trehalose; tuberculosis
Abstract:
... Tuberculosis (TB) disease is a global epidemic caused by the pathogenic Mycobacterium tuberculosis (Mtb). Tools that can track the replication status of viable Mtb cells within macrophages are vital for the elucidation of host–pathogen interactions. Here, we present a cephalosphorinase-dependent green trehalose (CDG-Tre) fluorogenic probe that enables fluorescence labeling of single live Bacille C ...
DOI:
10.1021/jacs.0c07700

24. A Glimpse into the Microbiota of Marketed Ready-to-Eat Crickets (Acheta domesticus)

Author:
Vesna Milanović; Federica Cardinali; Lucia Aquilanti; Cristiana Garofalo; Andrea Roncolini; Riccardo Sabbatini; Francesca Clementi; Andrea Osimani
Source:
Indian journal of microbiology 2020 v.60 no.1 pp. 115-118
ISSN:
0046-8991
Subject:
Acheta domesticus; Clostridium; European Union; Hydrogenophilus; Lysobacter; Pseudomonas; Ruminococcus; Staphylococcus; bacteria; commercialization; denaturing gradient gel electrophoresis; enzymes; genes; insects; polymerase chain reaction; ready-to-eat foods; rearing; ribosomal RNA; Austria; Belgium; France; Netherlands
Abstract:
... The present study was aimed to get an insight into the bacterial biota of ready-to-eat small crickets (Acheta domesticus) already marketed in the European Union. 16S rRNA gene of the DNAs extracted from thirty-two samples of ready-to-eat crickets commercialized by 4 European Union producers located in Austria, Belgium, France and the Netherlands (2 batches per producer) was analyzed by Polymerase ...
DOI:
10.1007/s12088-019-00817-x
PubMed:
32089581
PubMed Central:
PMC7000585

25. A Gnotobiotic Model to Examine Plant and Microbiome Contributions to Survival under Arsenic Stress

Author:
María del Carmen Molina; James F. White; Sara García-Salgado; M. Ángeles Quijano; Natalia González-Benítez
Source:
Microorganisms 2020 v.9 no.1 pp. -
ISSN:
2076-2607
Subject:
Arthrobacter; Pantoea; arsenic; bacteria; endophytes; exhibitions; germ-free animals; germination; hairs; microbiome; models; root growth; root hairs; seedling growth; seedlings; seeds; Montana
Abstract:
... So far, the relative importance of the plant and its microbiome in the development of early stages of plant seedling growth under arsenic stress has not been studied. To test the role of endophytic bacteria in increasing plant success under arsenic stress, gnotobiotic seeds of J. montana were inoculated with two endophytic bacteria: Pantoea conspicua MC-K1 (PGPB and As resistant bacteria) and Arth ...
DOI:
10.3390/microorganisms9010045

26. A Grain Rot of Rice in Iran Caused by a Xanthomonas Strain Closely Related to X. sacchari

Author:
S. Ali Mirghasempour; Shiwen Huang; David J. Studholme; Carrie L. Brady
Source:
Plant disease 2020 v.104 no.6 pp. 1581-1583
ISSN:
0191-2917
Subject:
Oryza sativa; Xanthomonas sacchari; bacteria; rice; sequence analysis; Iran
Abstract:
... Rice grain rot disease was detected for the first time in Mazandaran Province, Iran. The bacteria isolated from infected rice plants showed grains rotted and darkening. A Xanthomonas strain closely connected to X. sacchari was identified using molecular and whole genome sequencing approaches confirmed as the causal agent by fulfilling Koch’s postulates. ...
DOI:
10.1094/PDIS-01-20-0179-SC

27. A High-Fat Diet Increases Gut Microbiota Biodiversity and Energy Expenditure Due to Nutrient Difference

Author:
Botao Wang; Qingmin Kong; Xiu Li; Jianxin Zhao; Hao Zhang; Wei Chen; Gang Wang
Source:
Nutrients 2020 v.12 no.10 pp. -
ISSN:
2072-6643
Subject:
Bifidobacterium; Chiroptera; Desulfovibrionaceae; Lactobacillus; Mucispirillum; bacteria; biochemical pathways; biodiversity; brown adipose tissue; carbohydrate metabolism; energy expenditure; energy metabolism; fiber content; genes; glucose; heat production; high fat diet; homeostasis; inflammation; intestinal microorganisms; intestines; low fat diet; males; metabolites; mice; nutrient intake; nutrients; obesity; pathogens; risk; sequence analysis; short chain fatty acids
Abstract:
... A high-fat diet (HFD) can easily induce obesity and change the gut microbiota and its metabolites. However, studies on the effects of high-fat diets on the host have drawn inconsistent results. In this study, the unexpected results showed that the refined HFD increased gut microbiota diversity and short-chain fatty acids (SCFAs), causing an increase in energy metabolism. Further analysis revealed ...
DOI:
10.3390/nu12103197

28. A Hungatella effluvii isolate in blood culture of a patient with hematochezia

Author:
Jae Hyeon Park; Taek Soo Kim; Hyunwoong Park
Source:
Anaerobe 2020 v.63 pp. 102211
ISSN:
1075-9964
Subject:
anaerobes; bacteremia; bacteria; blood; human diseases; intestinal microorganisms; matrix-assisted laser desorption-ionization mass spectrometry; patients; prostatic neoplasms; ribosomal RNA; sequence analysis
Abstract:
... Hungatella spp. are anaerobic bacteria, are known members of the gut microbiome and very rarely cause human infection. Hungatella effluvii was isolated from an effluent treatment plant in 2014. We report a case of bacteremia due to H. effluvii that occurred after hematochezia in a patient with prostate cancer. It was misidentified by the VITEK 2 system (bioMérieux, France) and matrix-assisted lase ...
DOI:
10.1016/j.anaerobe.2020.102211

29. A Key Glycine in Bacterial Steroid-Degrading Acyl-CoA Dehydrogenases Allows Flavin-Ring Repositioning and Modulates Substrate Side Chain Specificity

Author:
Alexander J. Stirling; Stephanie E. Gilbert; Megan Conner; Evan Mallette; Matthew S. Kimber; Stephen Y. K. Seah
Source:
Biochemistry 2020 v.59 no.42 pp. 4081-4092
ISSN:
1520-4995
Subject:
Mycobacterium tuberculosis; Thermomonospora curvata; active sites; acyl coenzyme A; bacteria; cholesterol; degradation; enzymes; eukaryotic cells; genes; metabolites; models; oxidation; saprophytes
Abstract:
... A wide variety of steroid metabolites synthesized by eukaryotes are all ultimately catabolized by bacteria; while generally saprophytic, pathogenic Mycobacteria have repurposed these pathways to utilize host intracellular cholesterol pools. Steroid degradation is complex, but a recurring theme is that cycles of β-oxidation are used to iteratively remove acetyl- or propanoyl-CoA groups. These β-oxi ...
DOI:
10.1021/acs.biochem.0c00568

30. A MATE Transporter is Involved in Pathogenicity and IAA Homeostasis in the Hyperplastic Plant Pathogen Pseudomonas savastanoi pv. nerii

Author:
Stefania Tegli; Lorenzo Bini; Silvia Calamai; Matteo Cerboneschi; Carola Biancalani
Source:
Microorganisms 2020 v.8 no.2 pp. -
ISSN:
2076-2607
Subject:
Pseudomonas syringae pv. savastanoi; active transport; bacteria; computer simulation; extrusion; gene expression; homeostasis; indole acetic acid; mutagenesis; pathogenicity; plant disease control; plant pathogens; toxic substances
Abstract:
... During the last years, many evidences have been accumulating about the phytohormone indole-3-acetic acid (IAA) as a multifaceted compound in the microbial world, with IAA playing a role as a bacterial intra and intercellular signaling molecule or as an effector during pathogenic or beneficial plant–bacteria interactions. However, pretty much nothing is known on the mechanisms that bacteria use to ...
DOI:
10.3390/microorganisms8020156

31. A Mesohaline Thraustochytrid Produces Extremely Halophilic Alpha-Amylases

Author:
Priyanka V. Shirodkar; Usha Devi Muraleedharan; Samir Damare; Seshagiri Raghukumar
Source:
Marine biotechnology 2020 v.22 no.3 pp. 403-410
ISSN:
1436-2228
Subject:
alpha-amylase; ammonium sulfate; bacteria; biotechnology; fractionation; gel chromatography; mangrove ecosystems; polyacrylamide gel electrophoresis; proteins; salinity; salt tolerance; seawater; solvents
Abstract:
... Halophilic bacteria are well known to produce highly salt-tolerant enzymes that have unusual applications in biotechnology. Production of halophilic proteins is generally not expected in mesohaline microorganisms. Ulkenia sp. AH-2, a mesohaline, marine straminipilan thraustochytrid isolated from waters of a mangrove ecosystem, produces halophilic alpha-amylases. Four enzyme fractions, viz.., A, B, ...
DOI:
10.1007/s10126-020-09960-9

32. A Multi-strain Potential Probiotic Formulation of GABA-Producing Lactobacillus plantarum 90sk and Bifidobacterium adolescentis 150 with Antidepressant Effects

Author:
R. A. Yunes; E. U. Poluektova; E. V. Vasileva; M. V. Odorskaya; M. V. Marsova; G. I. Kovalev; V. N. Danilenko
Source:
Probiotics and antimicrobial proteins 2020 v.12 no.3 pp. 973-979
ISSN:
1867-1306
Subject:
Bifidobacterium adolescentis; Lactobacillus plantarum; antidepressants; bacteria; central nervous system; cognition; fluoxetine; forced swimming test; gamma-aminobutyric acid; mice; neurotransmitters; nociception; oral administration; probiotics
Abstract:
... Today, a number of studies conclusively show that certain bacterial strains, mainly from the genera Lactobacillus and Bifidobacterium, influence the functioning of the central nervous system, leading to changes in beahvior, nociception and the cognitive abilities of humans and animals. Such strains serve as the basis for developing probiotics with a curative potential for the central nervous syste ...
DOI:
10.1007/s12602-019-09601-1

33. A Nanostructured Gold/Graphene Microfluidic Device for Direct and Plasmonic-Assisted Impedimetric Detection of Bacteria

Author:
Roozbeh Siavash Moakhar; Tamer AbdelFatah; Alireza Sanati; Mahsa Jalali; Sarah Elizabeth Flynn; Sahar Sadat Mahshid; Sara Mahshid
Source:
ACS applied materials & interfaces 2020 v.12 no.20 pp. 23298-23310
ISSN:
1944-8252
Subject:
Escherichia coli; Pseudomonas putida; Staphylococcus epidermidis; bacteria; contact angle; detection limit; dielectric spectroscopy; electric field; electrochemistry; gold; graphene; light; nanogold; nanosheets; sensors (equipment); wettability
Abstract:
... Hierarchical 3D gold nano-/microislands (NMIs) are favorably structured for direct and probe-free capture of bacteria in optical and electrochemical sensors. Moreover, their unique plasmonic properties make them a suitable candidate for plasmonic-assisted electrochemical sensors, yet the charge transfer needs to be improved. In the present study, we propose a novel plasmonic-assisted electrochemic ...
DOI:
10.1021/acsami.0c02654

34. A New Approach to Producing High Yields of Pulcherrimin from Metschnikowia Yeasts

Author:
Ewelina Pawlikowska; Beata Kolesińska; Maria Nowacka; Dorota Kregiel
Source:
Fermentation 2020 v.6 no.4 pp. -
ISSN:
2311-5637
Subject:
Metschnikowia; bacteria; biofilm; carbon; culture media; ecosystems; fermentation; glucose; iron; nuclear magnetic resonance spectroscopy; polysorbates; purity; radiation resistance; strains; yeasts; yields
Abstract:
... Pulcherrimin, a red iron chelate, is produced by some yeasts and bacteria. It plays important ecological roles in many ecosystems, including growth control, biofilm inhibition and photoprotection. In this study, fifteen yeast strains of the genus Metschnikowia were characterized based on their production of pulcherrimin. Yeast pulcherrimin was isolated and its purity assessed using ¹H nuclear magn ...
DOI:
10.3390/fermentation6040114

35. A New Type of Chronic Wound Infection after Wisdom Tooth Extraction: A Diagnostic Approach with 16S-rRNA Gene Analysis, Next-Generation Sequencing, and Bioinformatics

Author:
Sebastian Böttger; Silke Zechel-Gran; Philipp Streckbein; Michael Knitschke; Torsten Hain; Markus Weigel; Jan-Falco Wilbrand; Eugen Domann; Hans-Peter Howaldt; Sameh Attia
Source:
Pathogens 2020 v.9 no.10 pp. -
ISSN:
2076-0817
Subject:
Alcanivorax; Delftia; bacteria; bacterial infections; bioinformatics; diagnostic techniques; extraction; flora; genes; high-throughput nucleotide sequencing; infection; inflammation; laboratories; microbiome; pathogens; postoperative complications; teeth; tissues; water; water pollution
Abstract:
... Delayed-onset infections are rare postoperative complications of lower third molar extractions. This article presents a case of a chronic combined hard and soft tissue infection after the extraction of a third molar, where the causative organisms could only be elucidated by molecular methods. Experimental 16S-rRNA gene analysis with next-generation sequencing and bioinformatics was used to identif ...
DOI:
10.3390/pathogens9100798

36. A Novel Neorickettsial Infection in 3 Dogs in the Pacific Northwest

Author:
Gabrielle Pastenkos; Kevin Snekvik; Dan Bradway; Ilaria Cerchiaro; Susan Mehain; Denise Krytenberg; Chrissy Eckstrand
Source:
Veterinary pathology 2020 v.57 no.2 pp. 286-289
ISSN:
1544-2217
Subject:
DNA; Neorickettsia helminthoeca; Neorickettsia risticii; Potomac horse fever; animal pathology; anorexia; bacteria; diarrhea; disease severity; dog diseases; dogs; enteritis; hepatitis; histopathology; lymphadenitis; necropsy; necrosis; poisoning; salmon; sequence analysis; signs and symptoms (animals and humans); therapeutics; Washington (state)
Abstract:
... The genus Neorickettsia includes obligate, intracellular bacteria responsible for diseases including Potomac horse fever caused by Neorickettsia risticii and salmon poisoning disease (SPD) caused by Neorickettsia helminthoeca. The Stellanchasmus falcatus (SF) agent is a member of this genus previously associated only with mild clinical signs in dogs. Between 2013 and 2016, 3 dogs in Washington Sta ...
DOI:
10.1177/0300985820901331

37. A Probiotic Preparation Hydrolyzes Gliadin and Protects Intestinal Cells from the Toxicity of Pro-Inflammatory Peptides

Author:
Alessandra Giorgi; Rugiada Cerrone; Daniela Capobianco; Simone Filardo; Patrizia Mancini; Flavia Zanni; Sergio Fanelli; Paola Mastromarino; Luciana Mosca
Source:
Nutrients 2020 v.12 no.2 pp. -
ISSN:
2072-6643
Subject:
Bifidobacterium; Lactobacillus; bacteria; celiac disease; digestion; filtration; gliadin; gluten; human cell lines; hydrolysates; hydrolysis; in vitro digestion; interleukin-6; intestines; mixtures; molecular weight; nutrients; occludins; oxidative stress; polyacrylamide gel electrophoresis; probiotics; protective effect; proteolysis; reversed-phase high performance liquid chromatography; strains; tight junctions; toxicity
Abstract:
... Celiac disease (CD) is an autoimmune enteropathy caused by an intolerance to gluten proteins. It has been hypothesized that probiotic bacteria may exert beneficial effects by modulating inflammatory processes and by sustaining peptide hydrolysis at the intestinal level. This study aims at evaluating the capacity of a probiotic mixture (two different strains of lactobacilli and three of bifidobacte ...
DOI:
10.3390/nu12020495

38. A Rare Cause of a Scrotal Abscess due to the Symbiotic Infection of Gardnerella vaginalis and Prevotella bivia in an Adult Male

Author:
Anthony Bekasiak; Fabian Dammann; Claudia Nader
Source:
Pathogens 2020 v.9 no.2 pp. -
ISSN:
2076-0817
Subject:
Gardnerella vaginalis; Prevotella bivia; abscess; adults; bacteria; males; men; mixed infection; pathogens; symbiosis; women
Abstract:
... Gardnerella vaginalis (G. vaginalis) is the major bacteria detected in women with bacterial vaginosis (BV). Prevotella bivia (P. bivia) has been demonstrated to show a symbiotic relationship with G. vaginalis. Some men have been shown to be colonized with G. vaginalis in their urogenital or anorectal tracts, however genitourinary infections in males, including balanitis and urethritis, due to this ...
DOI:
10.3390/pathogens9020093

39. A Rare Glimpse into the Past of the Anthrax Pathogen Bacillus anthracis

Author:
Peter Braun; Mandy Knüpfer; Markus Antwerpen; Dagmar Triebel; Gregor Grass
Source:
Microorganisms 2020 v.8 no.2 pp. -
ISSN:
2076-2607
Subject:
Bacillus anthracis; DNA; anthrax; bacteria; cattle; chromosomes; genomics; isolation techniques; pathogens; phylogeny; zoonoses; Central European region; Germany
Abstract:
... The bacterium Bacillus anthracis is the causative agent of the zoonotic disease anthrax. While genomics of extant B. anthracis isolates established in-depth phylogenomic relationships, there is scarce information on the historic genomics of the pathogen. Here, we characterized the oldest documented B. anthracis specimen. The inactive 142-year-old material originated from a bovine diseased in Chemn ...
DOI:
10.3390/microorganisms8020298

40. A Review of Asbestos Bioweathering by Siderophore-Producing Pseudomonas: A Potential Strategy of Bioremediation

Author:
Sébastien R. David; Valérie A. Geoffroy
Source:
Microorganisms 2020 v.8 no.12 pp. -
ISSN:
2076-2607
Subject:
Pseudomonas; asbestos; bacteria; bioremediation; cement; chelation; gypsum; humans; iron; siderophores; soil; solubilization; toxicity; weathering
Abstract:
... Asbestos, silicate minerals present in soil and used for building constructions for many years, are highly toxic due primarily to the presence of high concentrations of the transition metal iron. Microbial weathering of asbestos occurs through various alteration mechanisms. Siderophores, complex agents specialized in metal chelation, are common mechanisms described in mineral alteration. Solubiliz ...
DOI:
10.3390/microorganisms8121870

41. A Review of Salmonella and Campylobacter in Broiler Meat: Emerging Challenges and Food Safety Measures

Author:
Hudson T. Thames; Anuraj Theradiyil Sukumaran
Source:
Foods 2020 v.9 no.6 pp. -
ISSN:
2304-8158
Subject:
Campylobacter; HACCP; Salmonella; animal source protein; anti-infective agents; bacteria; bacterial contamination; cetylpyridinium chloride; chicken meat; chlorine; chlorites; industry; methodology; nonthermal processing; peracetic acid; poultry; sodium; spraying; United States
Abstract:
... Poultry is one of the largest sources of animal-based protein in the United States. Poultry processing has grown from a small local network of plants to nearly 500 plants nationwide. Two of the most persistent bacteria in poultry processing are Salmonella and Campylobacter. It was not until the introduction of Hazard Analysis and Critical Control Point systems in 1996 that major efforts to reduce ...
DOI:
10.3390/foods9060776

42. A Review: Wolbachia-Based Population Replacement for Mosquito Control Shares Common Points with Genetically Modified Control Approaches

Author:
Pei-Shi Yen; Anna-Bella Failloux
Source:
Pathogens 2020 v.9 no.5 pp. -
ISSN:
2076-0817
Subject:
Culicidae; Wolbachia; arboviruses; bacteria; control methods; diet; environmental factors; insect vectors; knowledge; mosquito control; mutants; pathogens; phenotype; population; public health; reliability; rendering; risk; temperature; vaccines; vector control
Abstract:
... The growing expansion of mosquito vectors has made mosquito-borne arboviral diseases a global threat to public health, and the lack of licensed vaccines and treatments highlight the urgent need for efficient mosquito vector control. Compared to genetically modified control strategies, the intracellular bacterium Wolbachia, endowing a pathogen-blocking phenotype, is considered an environmentally fr ...
DOI:
10.3390/pathogens9050404

43. A Rice NBS-ARC Gene Conferring Quantitative Resistance to Bacterial Blight Is Regulated by a Pathogen Effector-Inducible miRNA

Author:
Guanghuai Jiang; Dongfeng Liu; Dedong Yin; Zhuangzhi Zhou; Yue Shi; Chunrong Li; Lihuang Zhu; Wenxue Zhai
Source:
Molecular plant 2020 v.13 no.12 pp. 1752-1767
ISSN:
1674-2052
Subject:
DNA methylation; bacteria; binding sites; blight; breeding; crop losses; cultivars; disease resistance; evolution; genes; innate immunity; landraces; microRNA; models; molecular cloning; pathogens; quantitative trait loci; sequence analysis; wild rice
Abstract:
... The bacterium Xanthomonas oryzae pv. Oryzae (Xoo) causes blight in rice worldwide, resulting in significant crop loss. However, no gene underlying a quantitative trait locus (QTL) for resistance against Xoo has been cloned yet. Here, we report the map-based cloning of a QTL, in which the NBS8R gene confers quantitative resistance to Xoo. NBS8R encodes an NB-ARC protein, which is involved in pathog ...
DOI:
10.1016/j.molp.2020.09.015

44. A Self-Calibrating Surface-Enhanced Raman Scattering-Active System for Bacterial Phenotype Detection

Author:
Huizhen Yu; Mingshu Xiao; Wei Lai; Md. Fazle Alam; Weijia Zhang; Hao Pei; Ying Wan; Li Li
Source:
Analytical chemistry 2020 v.92 no.6 pp. 4491-4497
ISSN:
1520-6882
Subject:
Raman spectroscopy; bacteria; bacterial infections; biochemical compounds; biomedical research; diagnostic techniques; gold; human health and safety; microbial detection; morbidity; nanoflowers; nanogold; nucleic acid hybridization; pathogens; patients; phenotype; quantitative analysis; survival rate
Abstract:
... Pathogen detection is of significant importance in human health and safety due to the high morbidity and mortality induced by bacterial infections. Therefore, the development of rapid, sensitive, and selective methods for the discrimination of pathogens is the key to improve the patient survival rates. In this work, we develop a new self-calibrating surface-enhanced Raman scattering (SERS)-based s ...
DOI:
10.1021/acs.analchem.9b05614

45. A Skeptic's Guide to Bacterial Mechanosensing

Author:
Ravi Chawla; Rachit Gupta; Tanmay P. Lele; Pushkar P. Lele
Source:
Journal of molecular biology 2020 v.432 no.2 pp. 523-533
ISSN:
0022-2836
Subject:
bacteria; drug resistance; forces; kinesin; mechanical loads; virulence
Abstract:
... Surface sensing in bacteria is a precursor to the colonization of biotic and abiotic surfaces, and an important cause of drug resistance and virulence. As a motile bacterium approaches and adheres to a surface from the bulk fluid, the mechanical forces that act on it change. Bacteria are able to sense these changes in the mechanical load through a process termed mechanosensing. Bacterial mechanose ...
DOI:
10.1016/j.jmb.2019.09.004
PubMed:
31629771
PubMed Central:
PMC7002054

46. A Slippery Scaffold: Synthesis and Recycling of the Bacterial Cell Wall Carrier Lipid

Author:
Sean D. Workman; Natalie C.J. Strynadka
Source:
Journal of molecular biology 2020 v.432 no.18 pp. 4964-4982
ISSN:
0022-2836
Subject:
alkyl (aryl) transferases; bacteria; biosynthesis; cell walls; cytoplasm; dephosphorylation; lipids; oligosaccharides; peptidoglycans; phosphates; plasma membrane
Abstract:
... The biosynthesis of bacterial cell envelope polysaccharides such as peptidoglycan relies on the use of a dedicated carrier lipid both for the assembly of precursors at the cytoplasmic face of the plasma membrane and for the translocation of lipid linked oligosaccharides across the plasma membrane into the periplasmic space. This dedicated carrier lipid, undecaprenyl phosphate, results from the dep ...
DOI:
10.1016/j.jmb.2020.03.025

47. A Strip-Till One-Pass System as a Component of Conservation Agriculture

Author:
Iwona Jaskulska; Kestutis Romaneckas; Dariusz Jaskulski; Piotr Wojewódzki
Source:
Agronomy 2020 v.10 no.12 pp. -
ISSN:
2073-4395
Subject:
administrative management; aggregate stability; agricultural conservation practice; agronomy; bacteria; carbon dioxide; conventional tillage; crop rotation; diameter; disturbed soils; earthworms; emissions; energy use and consumption; field experimentation; fuels; fungi; glomalin; labor; minimum tillage; occurrence; plant residues; reduced tillage; row spacing; runoff; soil aggregates; soil erosion; soil structure; soil water; sowing; strip tillage; technology; water content; weight
Abstract:
... Conservation agriculture has three main pillars, i.e., minimum tillage, permanent soil cover, and crop rotation. Covering the soil surface with plant residues and minimum mechanical soil disturbance can all result from introducing a strip-till one-pass (ST-OP) system. The aim of this study was to determine the impact of the ST-OP technology on the management of plant residues, soil properties, inp ...
DOI:
10.3390/agronomy10122015

48. A Tale of Two Transitions: The Unfolding Mechanism of the prfA RNA Thermosensor

Author:
Huaqun Zhang; Ian Hall; Amos J. Nissley; Kyrillos Abdallah; Sarah C. Keane
Source:
Biochemistry 2020 v.59 no.48 pp. 4533-4545
ISSN:
1520-4995
Subject:
Listeria monocytogenes; Ursidae; bacteria; binding sites; genes; messenger RNA; ribosomes; temperature; thermal stability; transcription factors; virulence
Abstract:
... RNA thermosensors (RNATs), found in the 5′ untranslated region (UTR) of some bacterial messenger RNAs (mRNAs), control the translation of the downstream gene in a temperature-dependent manner. In Listeria monocytogenes, the expression of a key transcription factor, PrfA, is mediated by an RNAT in its 5′ UTR. PrfA functions as a master regulator of virulence in L. monocytogenes, controlling the exp ...
DOI:
10.1021/acs.biochem.0c00588

49. A Two-Component System Acquired by Horizontal Gene Transfer Modulates Gene Transfer and Motility via Cyclic Dimeric GMP

Author:
Purvikalyan Pallegar; Marta Canuti; Evan Langille; Lourdes Peña-Castillo; Andrew S. Lang
Source:
Journal of molecular biology 2020 v.432 no.17 pp. 4840-4855
ISSN:
0022-2836
Subject:
Rhodobacter capsulatus; Rhodobacter sphaeroides; Sphingomonadales; bacteria; cyclic GMP; enzyme activity; gene transfer; genes; histidine kinase; horizontal gene transfer; phosphorylation; proteins
Abstract:
... Bis-(3′–5′)-cyclic dimeric guanosine monophosphate (c-di-GMP) is an important intracellular signaling molecule that affects diverse physiological processes in bacteria. The intracellular levels of c-di-GMP are controlled by proteins acting as diguanylate cyclase (DGC) and phosphodiesterase (PDE) enzymes that synthesize and degrade c-di-GMP, respectively. In the alphaproteobacterium Rhodobacter cap ...
DOI:
10.1016/j.jmb.2020.07.001

50. A YajQ‐LysR‐like, cyclic di‐GMP‐dependent system regulating biosynthesis of an antifungal antibiotic in a crop‐protecting bacterium, Lysobacter enzymogenes

Author:
Sen Han; Danyu Shen; Yu‐Chuan Wang; Shan‐Ho Chou; Mark Gomelsky; Yong‐Gui Gao; Guoliang Qian
Source:
Molecular plant pathology 2020 v.21 no.2 pp. 218-229
ISSN:
1464-6722
Subject:
DNA; Lysobacter enzymogenes; Xanthomonas campestris; antibiotics; bacteria; binding proteins; biological control agents; biosynthesis; fungi; heat stability; operon; pathogens; promoter regions; second messengers; transcription (genetics); transcription factors; virulence
Abstract:
... YajQ, a binding protein of the universal bacterial second messenger cyclic di‐GMP (c‐di‐GMP), affects virulence in several bacterial pathogens, including Xanthomonas campestris. In this bacterium, YajQ interacts with the transcription factor LysR. Upon c‐di‐GMP binding, the whole c‐di‐GMP‐YajQ‐LysR complex is found to dissociate from DNA, resulting in virulence gene regulation. Here, we identify a ...
DOI:
10.1111/mpp.12890
PubMed:
31747123
PubMed Central:
PMC6988422

51. A bacterial vaccine polypeptide protective against nontypable Haemophilus influenzae

Author:
Paul W. Whitby; Daniel J. Morton; Huda J. Mussa; Lucia Mirea; Terrence L. Stull
Source:
Vaccine 2020 v.38 no.14 pp. 2960-2970
ISSN:
0264-410X
Subject:
Chinchilla (Chinchillidae); Haemophilus influenzae; adjuvants; affinity chromatography; alum; animal models; antibodies; antiserum; bacteremia; bacteria; bacterial vaccines; bioinformatics; enzyme-linked immunosorbent assay; immunoglobulin G; middle ear; otitis media; polypeptides; respiratory tract diseases; surface proteins; vaccine development; United States
Abstract:
... Nontypeable strains of Haemophilus influenzae (NTHi) are one of the most common cause of otitis media and the most frequent infection associated with exacerbations of chronic obstructive pulmonary disease; there is currently no vaccine in the U.S. to prevent NTHi. Using bioinformatics and structural vaccinology, we previously identified several NTHi species-conserved and sequence-conserved peptide ...
DOI:
10.1016/j.vaccine.2020.02.054

52. A bacterium against the tiger: preliminary evidence of fertility reduction after release of Aedes albopictus males with manipulated Wolbachia infection in an Italian urban area

Author:
Beniamino Caputo; Riccardo Moretti; Mattia Manica; Paola Serini; Elena Lampazzi; Marco Bonanni; Giulia Fabbri; Verena Pichler; Alessandra della Torre; Maurizio Calvitti
Source:
Pest management science 2020 v.76 no.4 pp. 1324-1332
ISSN:
1526-498X
Subject:
Aedes albopictus; Culex pipiens; Wolbachia; arboviruses; bacteria; cytoplasmic incompatibility; eggs; endosymbionts; females; field experimentation; insect control; insects; males; risk; sterilizing; temperate zones; urban areas; Italy
Abstract:
... BACKGROUND: Novel tools are needed to reduce the nuisance and risk of exotic arbovirus transmission associated with the colonization of temperate regions by Aedes albopictus. The incompatible insect technique (IIT) is a population suppression approach based on cytoplasmic incompatibility between males with manipulated endosymbionts and wild females. Here, we present the results of the first field ...
DOI:
10.1002/ps.5643

53. A bio-functions integration microcosm: Self-immobilized biochar-pellets combined with two strains of bacteria to remove atrazine in water and mechanisms

Author:
Tianmiao Yu; Li Wang; Fang Ma; Yujiao Wang; Shanshan Bai
Source:
Journal of hazardous materials 2020 v.384 pp. 121326
ISSN:
0304-3894
Subject:
Agrobacterium; Arthrobacter; Aspergillus niger; Fourier transform infrared spectroscopy; X-ray photoelectron spectroscopy; adsorption; atrazine; bacteria; biochar; biochemical pathways; biodegradation; biomass; biosorption; energy-dispersive X-ray analysis; fungi; kinetics; mechanism of action; models; pH; pellets; scanning electron microscopy; strength (mechanics); wastewater treatment; zeta potential
Abstract:
... A self-immobilization method for microorganisms was developed based on fungal pellets. Generally, pellets have some problems such as cell leakage, cell loading limitation and low mechanical strength. Therefore, biochar was applied to overcome these disadvantages. Atrazine degradable microorganism Arthrobacter sp. ZXY-2 was immobilized by Aspergillus niger Y3 pellets. After adding biochar with opti ...
DOI:
10.1016/j.jhazmat.2019.121326

54. A bio-inert and thermostable zwitterionic copolymer for the surface modification of PVDF membranes

Author:
Shuo-Hsi Tang; Antoine Venault; Chun Hsieh; Gian Vincent Dizon; Chen-Tsyr Lo; Yung Chang
Source:
Journal of membrane science 2020 v.598 pp. 117655
ISSN:
0376-7388
Subject:
X-ray photoelectron spectroscopy; antifouling activities; artificial membranes; bacteria; blood; coatings; composite polymers; filtration; hydrophilicity; nuclear magnetic resonance spectroscopy; styrene; sulfobetaine; thermal stability; thermoplastics; zwitterions
Abstract:
... This study explores the antifouling properties of membranes dip-coated with a copolymer formed by zwitterionization of poly(styrene-r-4-vinylpyridine), zP(S-r-4VP). 3 copolymers having a different chain length were produced. The NMR analysis showed that the styrene/4-vinylpyridine molar ratio was controlled to 64/36, while XPS tests revealed that the zwitterionization degree ranged between 70% and ...
DOI:
10.1016/j.memsci.2019.117655

55. A biocompatible PAA-Cu-MOP hydrogel for wound healing

Author:
Linlin Chen; Yu Qin; Jing Cheng; Yi Cheng; Zhixiang Lu; Xiaolan Liu; Shaoxiong Yang; Shuhan Lu; Liyan Zheng; Qiue Cao
Source:
RSC advances 2020 v.10 no.59 pp. 36212-36218
ISSN:
2046-2069
Subject:
angiogenesis; antibiotics; antimicrobial properties; bacteria; bacterial infections; behavior; biocompatibility; cell proliferation; epidermal growth factor; exhibitions; glycerol; humans; hydrogels; mixing; strains; tissue repair; ultrasonics
Abstract:
... Wounds infected by bacteria are dangerous for human beings. However, along with the emergence of new strains and strong bacterial resistance, traditional antibiotics are unable to meet the medical needs for treating bacterial infections. Thus, new antibacterial substances with superior antimicrobial properties are urgently needed. Herein, a hydrogel containing poly acrylic acid (PAA), glycerol and ...
DOI:
10.1039/c9ra10031h

56. A biophysiological perspective on enhanced nitrate removal from decentralized domestic sewage using gravitational-flow multi-soil-layering systems

Author:
Pei Song; Guohe Huang; Yongyuan Hong; Chunjiang An; Xiaying Xin; Peng Zhang
Source:
Chemosphere 2020 v.240 pp. 124868
ISSN:
0045-6535
Subject:
activated sludge; aerobes; bacteria; biofilm; denitrification; factor analysis; habitats; nitrates; oxygen consumption; polybutylene succinate; sewage; sewage treatment; soil; soil sampling; species abundance; species richness; total nitrogen; zeolites
Abstract:
... Multi-soil-layering (MSL) system with brick-wall pattern structure and gravitational flow can be used for decentralized rural domestic sewage treatment. The capability of soil for contaminant removal is maximized within soil mixture blocks (SMBs). However, the performance of removing nitrate was still not ideal during operation. To improve its performance in MSL system, the relationship between bi ...
DOI:
10.1016/j.chemosphere.2019.124868

57. A case of bacteriemic mediastinitis due to Prevotella buccae after cardiac surgery

Author:
C. Duployez; C. Loiez; R. Hund; B. Jegou; C. Decoene; F. Wallet
Source:
Anaerobe 2020 v.61 pp. 102097
ISSN:
1075-9964
Subject:
Prevotella buccae; anaerobes; bacteria; blood sampling; coronary vessels; surgery
Abstract:
... Mediastinitis is a well-known complication of open-heart surgery. Strictly anaerobic bacteria are rarely found in this condition, unlike in descending mediastinitis. We report the case of a mediastinitis due to Prevotella buccae after surgical replacement of the aortic valve and triple coronary artery bypass in an immunocompetent 76 year-old man. The bacteria were found in pure culture on blood sa ...
DOI:
10.1016/j.anaerobe.2019.102097

58. A case of feline gastrointestinal eosinophilic sclerosing fibroplasia limited to the mesentery

Author:
N. Kambe; R. Okabe; H. Osada; M. Ogawa; M. Kishimoto; R. Fukushima; H. Kondo; K. Ohmori
Source:
journal of small animal practice 2020 v.61 no.1 pp. 64-67
ISSN:
0022-4510
Subject:
anemia; anorexia; ascites; bacteria; cats; collagen; cyclosporine; eosinophilia; eosinophils; females; fibroblasts; gastrointestinal system; histology; laparotomy; mesentery; mixed breeds; neutrophils; peritonitis; prednisolone; remission; vomiting
Abstract:
... An entire, female, mixed‐breed cat of unknown age was presented with a 6‐week history of lethargy, anorexia and vomiting. There was an increase in the number of white blood cells in the blood, including neutrophils and eosinophils; moderate anaemia; ascites; and possible mesenteric peritonitis. Exploratory laparotomy revealed firm, multifocal small nodules in the mesentery. As the nodules were sur ...
DOI:
10.1111/jsap.12847
PubMed:
29708273
PubMed Central:
PMC7166917

59. A cephalosporin–chemiluminescent conjugate increases beta-lactamase detection sensitivity by four orders of magnitude

Author:
Santanu Maity; Xiaojian Wang; Subhamoy Das; Maomao He; Lee W. Riley; Niren Murthy
Source:
Chemical communications 2020 v.56 no.24 pp. 3516-3519
ISSN:
1364-548X
Subject:
bacteria; beta-lactamase; chemical compounds; chemical reactions; chemiluminescence; detection limit; drug resistance; enzyme activity; enzyme substrates; fluorescence
Abstract:
... The expression of beta-lactamases in bacteria is a central cause of drug resistance. In this report, we present a beta-lactamase chemiluminescent probe, termed CCP, which can for the first time detect beta-lactamase activity via chemiluminescence and can detect beta lactamase with a sensitivity that is 4-orders of magnitude higher than the commercially available fluorescent lactamase substrate flu ...
DOI:
10.1039/c9cc09498a

60. A cinnamaldehyde-based formulation as an alternative to sodium hypochlorite for post-harvest decontamination of citrus fruit

Author:
Caio F. C. Zamuner; Guilherme Dilarri; Lucia C. Bonci; Luiz L. Saldanha; Franklin Behlau; Tamiris G. S. Marin; Daiane C. Sass; Mauricio Bacci Jr.; Henrique Ferreira
Source:
Tropical plant pathology 2020 v.45 no.6 pp. 701-709
ISSN:
1983-2052
Subject:
Citrus; European Union; bacteria; chemical analysis; citrus canker; citrus fruits; control methods; copper; corrosion; decontamination; equipment; eugenol; food contamination; fruit quality; growers; isomers; laws and regulations; leafminers; organic matter; plant pathology; population; raw fruit; sodium hypochlorite; trees; tropical plants; windbreaks; Brazil
Abstract:
... According to the most recent regulation, published in 2018, areas or states of Brazil where citrus canker is endemic are no longer obliged to eradicate citrus trees affected by the disease as in the past 60 years. Instead, growers have to adopt a set of control measures, such as copper sprays, windbreaks, and control of the citrus leaf miner to minimize the impact of the disease on fruit quality a ...
DOI:
10.1007/s40858-020-00338-9

61. A class B scavenger receptor mediates antimicrobial peptide secretion and phagocytosis in Chinese mitten crab (Eriocheir sinensis)

Author:
Muxue Tang; Xuejie Li; Lei Yang; Qun Wang; Weiwei Li
Source:
Developmental and comparative immunology 2020 v.103 pp. 103496
ISSN:
0145-305X
Subject:
Eriocheir sinensis; Staphylococcus aureus; Vibrio parahaemolyticus; amino acids; antimicrobial peptides; bacteria; genes; hemocytes; immune response; pathogen-associated molecular patterns; pathogens; phagocytosis; polypeptides; receptors; secretion; tissues
Abstract:
... Scavenger receptors (SRs) are pattern recognition receptors (PRRs) vital for innate immunity. As well as their importance in immune recognition, microbe phagocytosis, and the clearance of modified endogenous molecules, they also activate downstream immune responses as co-receptors. In the current study, we identified a class B scavenger receptor in Eriocheir sinensis (EsSR-B2). The full-length gen ...
DOI:
10.1016/j.dci.2019.103496

62. A cleaner process of deinking waste paper pulp using Pseudomonas mendocina ED9 lipase supplemented enzyme cocktail

Author:
Vinod Kumar Nathan; Mary Esther Rani
Source:
Environmental science and pollution research international 2020 v.27 no.29 pp. 36498-36509
ISSN:
0944-1344
Subject:
Pseudomonas mendocina; ammonium sulfate; bacteria; carboxylic ester hydrolases; deinking; ecotoxicology; endo-1,4-beta-glucanase; enzyme activity; feces; lignocellulose; molecular weight; paper pulp; pollution; research; waste paper; xylanases
Abstract:
... Lipase enzyme has a critical role in deinking process along with other lignocellulosic enzymes. In this paper, we try to demonstrate the role of lipase in the enzyme cocktail used for enzymatic deinking. For this, we identified a potential lipolytic bacterium, Pseudomonas mendocina ED9 isolated from elephant dung with a molecular weight of 35 kDa. During the Box-Benhken model optimization, a maxim ...
DOI:
10.1007/s11356-020-09641-z

63. A clinical approach to multidrug-resistant urinary tract infection and subclinical bacteriuria in dogs and cats

Author:
T Johnstone
Source:
New Zealand veterinary journal 2020 v.68 no.2 pp. 69-83
ISSN:
1176-0710
Subject:
antibiotics; bacteria; bacterial infections; biomedical research; cats; dogs; guidelines; issues and policy; multiple drug resistance; pets; planning; therapeutics; urinary tract; urinary tract diseases
Abstract:
... Multidrug-resistant bacteria are increasingly isolated from the urinary tract of pets, particularly those that suffer from concurrent conditions, have been hospitalised, or were treated with antimicrobials in the recent past. Many of the multidrug-resistant bacteria encountered are resistant to all commonly used oral antibiotics. This poses both a therapeutic dilemma in the individual pet and a th ...
DOI:
10.1080/00480169.2019.1689196

64. A clinically important, plasmid-borne antibiotic resistance gene (β-lactamase TEM-116) present in desert soils

Author:
Yashini Naidoo; Angel Valverde; Errol D. Cason; Rian E. Pierneef; Don A. Cowan
Source:
Science of the total environment 2020 v.719 pp. 137497
ISSN:
0048-9697
Subject:
One Health initiative; anthropogenic activities; antibiotic resistance; antibiotic resistance genes; antibiotics; bacteria; beta-lactamase; desert soils; drugs; farming systems; habitats; humans; metagenomics; metal tolerance; metals; plasmids; probability; resistance mechanisms; soil ecology
Abstract:
... The exhaustive use of antibiotics in humans, animal farming and other agricultural practices has resulted in the frequent appearance of antibiotic resistant bacteria in human-impacted habitats. However, antibiotic resistance in natural (less-impacted) habitats is less understood. Using shotgun metagenomics we analysed soils from relatively low anthropogenic impact sites across the Namib Desert. We ...
DOI:
10.1016/j.scitotenv.2020.137497

65. A comparative adsorption study of aflatoxin B1 and aflatoxin G1 in almond butter fermented by Lactobacillus fermentum and Lactobacillus delbrueckii subsp. lactis

Author:
Seyed Mohammad Bagher Hashemi; Mohammad Javad Amiri
Source:
Lebensmittel-Wissenschaft + Technologie 2020 v.128 pp. 109500
ISSN:
0023-6438
Subject:
Lactobacillus delbrueckii subsp. lactis; Lactobacillus fermentum; adsorption; aflatoxin B1; aflatoxin G1; almond butter; bacteria; endothermy; feeds; fermentation; models; sorption isotherms; thermodynamics
Abstract:
... Due to worldwide problems of mycotoxins such as Aflatoxin B₁ (AFB₁) and aflatoxin G₁ (AFG₁) in feeds, there is a lot of attention for low costs, practical and high efficiency detoxification methods. The goal of the present study was to examine the capability of two bacteria including L. delbrueckii subsp. lactis and L. fermentum for detoxification of AFB₁ and AFG₁ in almond butter at 35, 37 and 39 ...
DOI:
10.1016/j.lwt.2020.109500

66. A comparative assessment of anti-inflammatory, anti-oxidant and anti-bacterial activities of hybrid and indigenous varieties of pumpkin (Cucurbita maxima Linn.) seed oil

Author:
M. Ziaul Amin; Tahera Islam Rity; M. Rasel Uddin; Md. Mashiar Rahman; M. Jashim Uddin
Source:
Biocatalysis and agricultural biotechnology 2020 v.28 pp. 101767
ISSN:
1878-8181
Subject:
2,2-diphenyl-1-picrylhydrazyl; Cucurbita maxima; Escherichia coli; Shigella sonnei; anti-inflammatory activity; antibacterial properties; antioxidant activity; antioxidants; bacteria; bovine serum albumin; denaturation; dose response; ethanol; hybrids; ingredients; inhibitory concentration 50; pumpkin oil; pumpkins
Abstract:
... Pumpkin seed is being used traditionally for curing and protecting various ailments. The present study was designed to evaluate the anti-inflammatory, anti-oxidant and anti-bacterial activities of ethanol extract of pumpkin (Cucurbita maxima Linn.) seed oil from hybrid and indigenous varieties. An anti-inflammatory, anti-oxidant, and anti-bacterial activities were assessed by BSA (bovine serum alb ...
DOI:
10.1016/j.bcab.2020.101767

67. A comparative evaluation of freezing criteria and molecular characterization of epiphytic ice-nucleating (Ice+) and non-ice-nucleating (Ice−) Pseudomonas syringae and Pseudomonas fluorescens

Author:
Bahareh Karimi; Rahim Nosrati; Bibi Sedigheh Fazly Bazzaz; Mirsasan Mirpour; Mohammadali Malboobi; Parviz Owlia
Source:
Journal of plant pathology 2020 v.102 no.1 pp. 169-178
ISSN:
1125-4653
Subject:
Citrus; Pseudomonas fluorescens; Pseudomonas syringae; Solanum lycopersicum; bacteria; epiphytes; freezing; frost injury; genes; ice nucleation; leaves; mutants; oranges; pH; pathogenicity; proteins; salt concentration; temperature; tomatoes
Abstract:
... The ice-nucleation-active bacteria that produce ice-nucleating proteins (INPs), especially Pseudomonas species, may play a critical potential role in frost injury. The ice-minus variant of bacteria is a mutant, lacking the genes responsible for the production of INPs. The aim of the project was the isolation of relevant bacteria. Pseudomonas-like bacteria were screened from 66 leaves samples and w ...
DOI:
10.1007/s42161-019-00402-7

68. A comparative study of biological activated carbon based treatments on two different types of municipal reverse osmosis concentrates

Author:
Shovana Pradhan; Linhua Fan; Felicity A. Roddick; Esmaeil Shahsavari; Andrew S. Ball; Xiaolei Zhang
Source:
Chemosphere 2020 v.240 pp. 124925
ISSN:
0045-6535
Subject:
activated carbon; bacteria; bacterial communities; coagulation; comparative study; denitrification; fluorescence; hydrogen peroxide; molecular weight; nitrates; nitrogen; nutrients; organic compounds; phosphorus; reverse osmosis; salinity; slaughterhouse wastes; streams; total nitrogen
Abstract:
... A study was conducted to understand the impact of reverse osmosis concentrate (ROC) characteristics on the efficacy of biological activated carbon (BAC) based treatments for removing organics and nutrients from two ROC streams (ROCₐ derived from municipal waste input with high salinity, and ROCb derived from domestic waste plus industrial trade waste with markedly lower salinity). Fluorescence exc ...
DOI:
10.1016/j.chemosphere.2019.124925

69. A comparative study of extraction techniques for maximum recovery of β-galactosidase from the yogurt bacterium Lactobacillus delbrueckii ssp. bulgaricus

Author:
Rabin Gyawali; Ayowole Oyeniran; Tahl Zimmerman; Sulaiman O. Aljaloud; Albert Krastanov; Salam A. Ibrahim
Source:
Journal of dairy research 2020 v.87 no.1 pp. 123-126
ISSN:
1469-7629
Subject:
Lactobacillus delbrueckii subsp. bulgaricus; bacteria; beta-galactosidase; comparative study; enzyme activity; mechanical methods; polyacrylamide gel electrophoresis; protein content; solvents; sonication; yogurt
Abstract:
... The study reported in this research communication evaluates the chemical (solvents) and mechanical (sonication, bead-beater) extraction methods to determine the maximum recovery of β-galactosidase from L. bulgaricus spp. Among all extraction techniques, sonication-assisted extraction yielded the highest amounts of enzyme activity (between 1892–2156 Miller Units) in cell-free extract (supernatant). ...
DOI:
10.1017/S0022029919001031

70. A comparative study on the use of selective media for the enumeration of Clostridium perfringens in poultry faeces

Author:
Martina Hustá; Richard Ducatelle; Freddy Haesebrouck; Filip Van Immerseel; Evy Goossens
Source:
Anaerobe 2020 v.63 pp. 102205
ISSN:
1075-9964
Subject:
Clostridium perfringens; antibiotic resistance; bacteria; color; comparative study; cycloserine; diagnostic techniques; feces; pathogenesis; poultry; selective media; sulfites; veterinary medicine
Abstract:
... Isolation and fast detection of Clostridium perfringens is essential in veterinary medical diagnostics and veterinary research, as it allows to recommend suitable treatment options after antimicrobial resistance determination, and is essential to study pathogenesis. In this study four selective media were tested for the enumeration of, and selectivity towards C. perfringens in faecal samples from ...
DOI:
10.1016/j.anaerobe.2020.102205

71. A comparison between whole mung bean and decorticated mung bean: beneficial effects on the regulation of serum glucose and lipid disorders and the gut microbiota in high-fat diet and streptozotocin-induced prediabetic mice

Author:
Dianzhi Hou; Qingyu Zhao; Laraib Yousaf; Borui Chen; Yong Xue; Qun Shen
Source:
Food & function 2020 v.11 no.6 pp. 5525-5537
ISSN:
2042-650X
Subject:
Bifidobacterium; Enterococcus; Staphylococcus; bacteria; blood glucose; blood lipids; blood proteins; blood serum; dysbiosis; fatty liver; genes; glucose tolerance; glycosylation; high fat diet; histology; insulin; intestinal microorganisms; lipid composition; lipids; metabolic diseases; mice; mung beans; protein content; sequence analysis; streptozotocin
Abstract:
... The aim of this study is to investigate the beneficial effects of whole mung bean (WMB) and decorticated mung bean (DMB) on the regulation of serum glucose and lipid disorders in high-fat diet (HFD) and streptozotocin (STZ)-induced prediabetic mice, and to further explore their gut microbiota modulatory effects. In the present study, the ability of mung bean-based diets to combat prediabetes-relat ...
DOI:
10.1039/d0fo00379d

72. A comparison of n-alkane contents in sediments of five lakes from contrasting environments

Author:
Yongdong Zhang; Jinlei Yu; Yaling Su; Yingxun Du; Zhengwen Liu
Source:
Organic geochemistry 2020 v.139 pp. 103943
ISSN:
0146-6380
Subject:
alkanes; alpine meadows; anthropogenic activities; bacteria; carbon; comparative study; eutrophication; lakes; limnology; macrophytes; paleontology; petroleum; sediments; watersheds; China
Abstract:
... In paleolimnology studies, the trajectories of n-alkane abundances and distributions in dated sediments are widely used indicators of changing environmental and ecological status in the overlying water over time. However, the factors contributing to the variable n-alkane profiles of lake systems are not yet fully understood. Here, a comparative study was conducted to examine natural and anthropoge ...
DOI:
10.1016/j.orggeochem.2019.103943

73. A comparison of novel inactivation methods for production of a vaccine against Streptococcus agalactiae in Nile tilapia Oreochromis niloticus

Author:
Fernando Carlos Ramos-Espinoza; Victor Alexander Cueva-Quiroz; Jefferson Yunis-Aguinaga; Julieta Rodini Engrácia de Moraes
Source:
Aquaculture 2020 v.528 pp. 735484
ISSN:
0044-8486
Subject:
Oreochromis niloticus; Streptococcus agalactiae; antibodies; bacteria; disease prevention; fingerlings; fish culture; formaldehyde; hydrogen peroxide; immunoglobulin M; inactivated vaccines; pH; pathogens; vaccination
Abstract:
... Disease prevention and control are crucial for the development of intensive commercial aquaculture. For some fish species, several vaccines are available to contain the effects of pathogens. However, in Nile tilapia there are just a few vaccines available. The current study compared the efficacy of three inactivated vaccines against Streptococcus agalactiae; in Nile tilapia. Formaldehyde, hydrogen ...
DOI:
10.1016/j.aquaculture.2020.735484

74. A competitive enzyme linked aptasensor with rolling circle amplification (ELARCA) assay for colorimetric detection of Listeria monocytogenes

Author:
Zhongxu Zhan; Hui Li; Ju Liu; Guoyang Xie; Fangbing Xiao; Xin Wu; Zoraida P. Aguilar; Hengyi Xu
Source:
Food control 2020 v.107 pp. 106806
ISSN:
0956-7135
Subject:
DNA; Listeria monocytogenes; aptasensors; bacteria; biotin; colorimetry; detection limit; enzyme substrates; food pathogens; human health; lettuce; oligonucleotides; peroxidase; streptavidin; virulent strains
Abstract:
... Listeria monocytogenes (L. monocytogenes) is a foodborne pathogen which can cause significant threats to human health, thus, a highly specific and accurate method for detection in food is urgently needed. In this study, an enzyme-linked aptasensor with rolling circle amplification (ELARCA) assay was developed for the sensitive and specific detection of L. monocytogenes. The assay was based on the ...
DOI:
10.1016/j.foodcont.2019.106806

75. A complete route for biodegradation of potentially carcinogenic cyanotoxin microcystin-LR in a novel indigenous bacterium

Author:
Fei Yang; Feiyu Huang; Hai Feng; Jia Wei; Isaac Yaw Massey; Geyu Liang; Fang Zhang; Lihong Yin; Sam Kacew; Xian Zhang; Yuepu Pu
Source:
Water research 2020 v.174 pp. 115638
ISSN:
0043-1354
Subject:
Sphingomonas; bacteria; biodegradation; carbon dioxide; carcinogenicity; cost effectiveness; ecosystems; eutrophication; lakes; microcystin-LR; multiomics; phenylacetic acid; public health; remediation; research; spectrometers; water
Abstract:
... Microcystin-leucine-arginine (MC-LR), a cyclic potentially carcinogenic hepatotoxin, occurs frequently in aquatic habitats worldwide and seriously threatens ecosystem and public health. Limited effectiveness of physicochemical treatments to remove MC-LR from drinking water has led to a search for alternative cost-effective and environment friendly biodegradation strategies. Obtaining MC-degrading ...
DOI:
10.1016/j.watres.2020.115638

76. A comprehensive assessment of microbiome diversity in Tenebrio molitor fed with polystyrene waste

Author:
Aneta K. Urbanek; Justyna Rybak; Magdalena Wróbel; Karol Leluk; Aleksandra M. Mirończuk
Source:
Environmental pollution 2020 v.262 pp. 114281
ISSN:
0269-7491
Subject:
Acidobacteria; Actinobacteria; Clostridia; Enterobacter; Enterococcus; Flavobacteria; Klebsiella; Lactococcus; Pseudomonas; Serratia; Tenebrio molitor; alpha-Proteobacteria; bacteria; biodegradability; bioplastics; breeding; carbon; high-throughput nucleotide sequencing; insect larvae; intestinal microorganisms; microbiome; packaging; polystyrenes; wastes
Abstract:
... Recently it was demonstrated that mealworm (Tenebrio molitor) larvae consume and biodegrade polystyrene. Thus, in this study a breeding investigation with various types of polystyrene was performed to follow the changes in the gut microbiome diversity. Polystyrene used for packaging purposes (PSp) and expanded polystyrene (EPS) were perceived as more favorable and attacked more frequently by mealw ...
DOI:
10.1016/j.envpol.2020.114281

77. A comprehensive review of bacterial osteomyelitis with emphasis on Staphylococcus aureus

Author:
Ahmad Nasser; Taher Azimi; Soheila Ostadmohammadi; Samaneh Ostadmohammadi
Source:
Microbial pathogenesis 2020 v.148 pp. 104431
ISSN:
0882-4010
Subject:
Staphylococcus aureus; adenosine triphosphate; adults; antibiotics; apoptosis; bacteria; biofilm; bones; children; chronic diseases; immune response; inflammation; necrosis; osteomyelitis; pathogens; phagocytes; secretion
Abstract:
... Osteomyelitis, a significant infection of bone tissue, gives rise to two main groups of infection: acute and chronic. These groups are further categorized in terms of the duration of infection. Usually, children and adults are more susceptible to acute and chronic infections, respectively. The aforementioned groups of osteomyelitis share almost 80% of the corresponding bacterial pathogens. Among a ...
DOI:
10.1016/j.micpath.2020.104431

78. A core microbiota of the plant-earthworm interaction conserved across soils

Author:
Samuel Jacquiod; Ruben Puga-Freitas; Aymé Spor; Arnaud Mounier; Cécile Monard; Christophe Mougel; Laurent Philippot; Manuel Blouin
Source:
Soil biology & biochemistry 2020 v.144 pp. 107754
ISSN:
0038-0717
Subject:
bacteria; clay; earthworms; fungi; hosts; microbial communities; microhabitats; quantitative polymerase chain reaction; sand; soil types
Abstract:
... The core microbiota defines the fraction of microorganisms common to all individuals from the same host species regardless of the abiotic context, be they located inside (e.g. animal guts) or outside (e.g. plant rhizospheres). While the core microbiota of many host species have been documented, no study attempted to decipher how these core microbiota could be altered when their respective host spe ...
DOI:
10.1016/j.soilbio.2020.107754

79. A correlation study on optimum conditions of microbial precipitation and prerequisites for self-healing concrete

Author:
Jing Xu; Yihong Tang; Xianzhi Wang
Source:
Process biochemistry 2020 v.94 pp. 266-272
ISSN:
1359-5113
Subject:
calcium; calcium carbonate; calcium nitrate; concrete; hydrolysis; mechanical properties; mixing; urea
Abstract:
... Microbial induced CaCO₃ precipitation (MICP) based upon enzymatic urea hydrolysis has been verified as an effective way for crack treatment, especially for self-healing of concrete cracks. This paper aimed at correlating optimum conditions of MICP with prerequisites for self-healing concrete. Orthogonal experiments on a combination of factors contributing to the MICP process were firstly performed ...
DOI:
10.1016/j.procbio.2020.04.028

80. A coupled bio-chemo-hydraulic model to predict porosity and permeability reduction during microbially induced calcite precipitation

Author:
Xuerui Wang; Udo Nackenhorst
Source:
Advances in water resources 2020 v.140 pp. 103563
ISSN:
0309-1708
Subject:
bacteria; calcite; chemical reactions; effective porosity; hydraulic conductivity; laboratory experimentation; models; permeability; soil mechanical properties; urease; water resources
Abstract:
... Microbially induced calcite precipitation (MICP) provides the potential for developing innovative and environmentally friendly techniques to improve the engineering properties of soil through reduction of permeability in soil and increase of soil stiffness and strength. In the present research work, coupled bio-chemo-hydraulic modelling was developed to enhance the understanding of the coupled pro ...
DOI:
10.1016/j.advwatres.2020.103563

81. A critical review of the relationship between dietary components, the gut microbe Akkermansia muciniphila, and human health

Author:
Muthukumaran Jayachandran; Stephen Sum Man Chung; Baojun Xu
Source:
Critical reviews in food science and nutrition 2020 v.60 no.13 pp. 2265-2276
ISSN:
1549-7852
Subject:
atherosclerosis; bacteria; diabetes mellitus; digestive system; food science; human health; humans; immunomodulation; intestinal microorganisms; prebiotics; therapeutics
Abstract:
... The human gut contains trillions of microorganisms with a great diversity that are associated with various health benefits. Recent studies have reported an increasing correlation between diet, gut microbiota, and human health, indicating rapid development in the field of gut health. Diet is an important factor that determines the gut microbiota composition. The gut comprises great diversities of m ...
DOI:
10.1080/10408398.2019.1632789

82. A cross sectional study of prevalence and risk factors associated with subclinical mastitis and intramammary infections, in dairy herds linked to milk collection centers in Rwanda

Author:
Jean Baptiste Ndahetuye; Janvier Twambazimana; Ann-Kristin Nyman; Callixte Karege; Michael Tukei; Martin Patrick Ongol; Ylva Persson; Renée Båge
Source:
Preventive veterinary medicine 2020 v.179 pp. 105007
ISSN:
0167-5877
Subject:
California mastitis test; Staphylococcus aureus; Staphylococcus chromogenes; Staphylococcus epidermidis; antibiotic resistance; bacteria; biosecurity; bovine mastitis; calves; clindamycin; cross-sectional studies; dairy cows; dairy herds; factor analysis; farmers; fermented milk; hygiene; lactation; legs; milk; milking; milking parlors; pathogens; penicillins; regression analysis; risk factors; screening; suckling; tetracycline; udder quarters; Rwanda
Abstract:
... The objective of this cross-sectional study was to investigate prevalence, causative udder pathogens and their antimicrobial resistance (AMR), as well as cow and herd risk factors associated with subclinical mastitis (SCM = cows with at least one udder quarter with california mastitis test (CMT) score > 2) and intramammary infections (IMI) caused by Staphylococcus(S.) aureus or Non aureus staphylo ...
DOI:
10.1016/j.prevetmed.2020.105007

83. A d-glucose- and d-xylose-tolerant GH1 β-glucosidase from Cellulosimicrobium funkei HY-13, a fibrolytic gut bacterium of Eisenia fetida

Author:
Do Young Kim; Jonghoon Kim; Sun Hwa Lee; Chungwook Chung; Dong-Ha Shin; Bon-Hwan Ku; Kwang-Hee Son; Ho-Yong Park
Source:
Process biochemistry 2020 v.94 pp. 282-288
ISSN:
1359-5113
Subject:
Cellulosimicrobium; Eisenia fetida; Escherichia coli; bacteria; beta-glucosidase; biocatalysts; calcium; catalytic activity; cobalt; dose response; earthworms; enzyme activity; galactose; gene overexpression; genes; glucose; glycosides; intestinal microorganisms; ions; manganese; mercury; nickel; pH; xylose
Abstract:
... The GluM gene (1491-bp) coding for a β-glucosidase comprising a single catalytic glycoside hydrolase family 1 domain from an earthworm (Eisenia fetida)-symbiotic bacterium, Cellulosimicrobium funkei HY-13, was cloned and over-expressed in Escherichia coli BL21. The recombinant histidine-tagged enzyme (rGluM: 56 kDa) displayed the highest cleavage activity toward p-nitrophenyl (pNP)-β-d-glucopyrano ...
DOI:
10.1016/j.procbio.2020.04.033

84. A disposable gold-cellulose nanofibril platform for SERS mapping

Author:
Saliha Nur Tanis; Hasan Ilhan; Burcu Guven; Emine Kubra Tayyarcan; Hakan Ciftci; Necdet Saglam; Ismail Hakki Boyaci; Ugur Tamer
Source:
Analytical methods 2020 v.12 no.24 pp. 3164-3172
ISSN:
1759-9679
Subject:
Bacillus subtilis; Enterobacter aerogenes; Escherichia coli; Micrococcus luteus; Raman spectroscopy; antibodies; bacteria; cellulose; chlorides; detection limit; gold; mixing; nanofibers; nanogold; nanorods; nitrobenzoic acids; plate count
Abstract:
... In this study, we present a disposable and inexpensive paper-like gold nanoparticle-embedded cellulose nanofibril substrate for the rapid enumeration of Escherichia coli (E. coli) using surface-enhanced Raman scattering (SERS) mapping. A disposable SERS substrate was simply constructed by mixing CNF and gold chloride solution at 120 °C in a water bath. The application of the resulting substrate wa ...
DOI:
10.1039/d0ay00662a

85. A dose response model for the inhalation route of exposure to P. aeruginosa

Author:
Kara Dean; Jade Mitchell
Source:
Microbial risk analysis 2020 v.15 pp. 100115
ISSN:
2352-3522
Subject:
Bayesian theory; Pseudomonas aeruginosa; aerosols; bacteria; control methods; dose response; humidifiers; inhalation exposure; lungs; microbiological risk assessment; models; risk; risk estimate; secondary infection
Abstract:
... This study develops a dose response model for Pseudomonas aeruginosa for the inhalation route of exposure using pre-existing data. P. aeruginosa is an opportunistic pathogen capable of causing community and hospital-acquired lung infections. As such, a dose response model for this route of exposure is needed to assess risks posed by the inhalation of aerosols from showers, humidifiers, or hot tubs ...
DOI:
10.1016/j.mran.2020.100115

86. A dual-functional implant with an enzyme-responsive effect for bacterial infection therapy and tissue regeneration

Author:
Yao Ding; Yansha Hao; Zhang Yuan; Bailong Tao; Maowen Chen; Chuanchuan Lin; Peng Liu; Kaiyong Cai
Source:
Biomaterials science 2020 v.8 no.7 pp. 1840-1854
ISSN:
2047-4849
Subject:
Staphylococcus aureus; animal experimentation; animal models; antibacterial properties; atomic absorption spectrometry; bacteria; bacterial infections; bones; endodeoxyribonucleases; ions; micro-computed tomography; nanoparticles; nanosilver; polyallylamines; polyamides; porous media; silica; silver; staining; therapeutics; tissue repair
Abstract:
... Biomaterial-associated bacterial infection is one of the major causes of implant failure. The treatment of such an implant infection typically requires the elimination of bacteria and acceleration of tissue regeneration around implants simultaneously. To address this issue, an ideal implanted material should have the dual functions of bacterial infection therapy and tissue regeneration at the same ...
DOI:
10.1039/c9bm01924c

87. A facile and universal strategy to endow implant materials with antibacterial ability via alkalinity disturbing bacterial respiration

Author:
Ji Tan; Zixiao Liu; Donghui Wang; Xianming Zhang; Shi Qian; Xuanyong Liu
Source:
Biomaterials science 2020 v.8 no.7 pp. 1815-1829
ISSN:
2047-4849
Subject:
alkalinity; bacteria; bacterial infections; cell proliferation; ceramics; cytotoxicity; electron transfer; electron transport chain; magnesium; magnesium oxide; osteoblasts; oxidative stress; polymers; titanium; zirconium oxide
Abstract:
... Multifarious strategies have been proposed to enhance the antibacterial ability of implant surfaces for preventing bacterial infection, however, developing facile and universal modification methods still remains extremely elusive. Herein, inspired by the fact that the electron transfer respiratory chain of bacteria is embedded in the membrane, we proposed a novel strategy of local alkalinity distu ...
DOI:
10.1039/c9bm01793c

88. A facile route to the preparation of antibacterial polysulfone-sulfonated polyethersulfone ultrafiltration membranes using a cationic surfactant cetyltrimethylammonium bromide

Author:
Aydın Cihanoğlu; Sacide Alsoy Altinkaya
Source:
Journal of membrane science 2020 v.594 pp. 117438
ISSN:
0376-7388
Subject:
Escherichia coli; Fourier transform infrared spectroscopy; Staphylococcus aureus; X-ray photoelectron spectroscopy; antibacterial properties; artificial membranes; bacteria; cationic surfactants; cetyltrimethylammonium bromide; contact angle; electrostatic interactions; gelation; leaching; micelles; molecular weight; permeability; polymers; sodium chloride; ultrafiltration
Abstract:
... Cetyltrimethylammonium bromide (CTAB), a cationic surfactant, is known to have strong bactericidal potential. In this study, we report a facile approach for preparing CTAB-containing polysulfone-sulfonated polyethersulfone (PSF-SPES) based ultrafiltration membranes with antibacterial properties. The CTAB was added in gelation medium at three different concentrations and made an electrostatic inter ...
DOI:
10.1016/j.memsci.2019.117438

89. A feasible scale-up production of Sporosarcina pasteurii using custom-built stirred tank reactor for in-situ soil biocementation

Author:
Armstrong I. Omoregie; Enzo A. Palombo; Dominic E.L. Ong; Peter M. Nissom
Source:
Biocatalysis and agricultural biotechnology 2020 v.24 pp. 101544
ISSN:
1878-8181
Subject:
Sporosarcina pasteurii; bacteria; bioprocessing; bioremediation; calcium carbonate; energy-dispersive X-ray analysis; enzyme activity; heavy metals; ingredients; pH; sand; scanning electron microscopy; soil; stainless steel; urease; yeast extract
Abstract:
... Production of urease enzyme from ureolytic bacteria (e.g. Sporosarcina pasteurii) is essential for precipitation of CaCO₃. This bioprocess is notably utilized for soil improvement, biocementation and bioremediation of heavy metals. Despite the viability for field-scale implementation as a suitable alternative to conventional treatment methods, most reports in the literature are constrained to labo ...
DOI:
10.1016/j.bcab.2020.101544

90. A fibrinogen-related protein (Mnfico3) acts as a novel pattern recognition receptor in Macrobrachium nipponense

Author:
Nan Qin; Mengjia Wu; Ting Tang; Fengsong Liu
Source:
Fish & shellfish immunology 2020 v.100 pp. 272-282
ISSN:
1050-4648
Subject:
Aeromonas veronii; Macrobrachium nipponense; RNA interference; amino acids; bacteria; binding capacity; cell-mediated immunity; complementary DNA; crustin; genes; hemocytes; ligands; muscles; open reading frames; pathogen-associated molecular patterns; phagocytosis; phylogeny; protein domains; recombinant proteins; shrimp; signal peptide; tissue distribution; vertebrates
Abstract:
... Fibrinogen-related proteins (FREPs) are widely found in both vertebrates as well as invertebrates, and they play a crucial role in host immunity. In this study, we isolated a novel ficolin gene (Mnfico3) from the oriental river prawn Macrobrachium nipponense. The complete cDNA sequence of Mnfico3 was 1133 bp long, containing an open reading frame of 765 bp coding for Mnfico3, a protein consisting ...
DOI:
10.1016/j.fsi.2020.02.066

91. A fish cytokine related to human IL-3, IL-5, and GM-CSF, induces development of eosinophil/basophil/mast-cell type (EBM) granulocytes

Author:
Kohei Nishiya; Mai Sawada; Johannes M. Dijkstra; Jiro Miyamae; Masaharu Okano; Fumihiko Katakura; Tadaaki Moritomo
Source:
Developmental and comparative immunology 2020 v.108 pp. 103671
ISSN:
0145-305X
Subject:
bacteria; basophils; carp; eosinophils; granulocyte-macrophage colony-stimulating factor; humans; interleukin-3; interleukin-5; mast cells; phytohemagglutinin; receptors; transcription factors
Abstract:
... Interleukin-3 (IL-3), IL-5, and granulocyte-macrophage colony-stimulating factor (GM-CSF) are related cytokines that signal through receptors possessing the β common (βc) chain. As a family, these cytokines combine rather non-specific hematopoietic growth factor properties with a special importance for eosinophils, basophils, and mast cells. In fish the cytokines of this family are called IL-5fam, ...
DOI:
10.1016/j.dci.2020.103671

92. A fluorescence quenching-recovery sensor based on RCA for the specific analysis of Fusobacterium nucleatum. nucleatum

Author:
Jingbo Jiao; Ping Li; Ying Gu; Xinjun Du; Shuo Wang; Junping Wang
Source:
Analytical biochemistry 2020 v.604 pp. 113808
ISSN:
0003-2697
Subject:
DNA-directed DNA polymerase; Fusobacterium nucleatum; bacteria; circular DNA; colorectal neoplasms; detection; digestion; fluorescence; genes; ligases; rolling; sampling; technology
Abstract:
... Fusobacterium nucleatum. nucleatum (Fn.n) is associated with colorectal carcinoma. A highly sensitive fluorescence quenching-recovery detection platform based on rolling circle amplification and hairpin molecular beacon technology for the specific analysis of Fn.n, hairpin MB being used to achieve double quenching. First, a specific recognition sequence target in the padlock probe was designed bas ...
DOI:
10.1016/j.ab.2020.113808

93. A four-CRD C-type lectin from razor clam Sinonovacula constricta mediates agglutination and phagocytosis

Author:
Tianyi Lan; Zhi Li; Maoxiao Peng; Donghong Niu; Yan Li; Jiale Li
Source:
Gene 2020 v.728 pp. 144287
ISSN:
0378-1119
Subject:
Veneroida; agglutination; bacteria; calcium; carbohydrate binding; carbohydrates; functional properties; genes; hemocytes; hepatopancreas; invertebrates; lectins; phagocytosis; signal peptide; testing; tissues
Abstract:
... C-type lectins are a superfamily of Ca²⁺-dependent carbohydrate-binding proteins that play crucial roles in invertebrate immunity. In this study, a novel C-type lectin gene (ScCTL-1) was identified in razor clam Sinonovacula constricta. The ScCTL-1 gene, consisting of four C-type carbohydrate recognition domains (CRDs) with an N-terminal signal peptide and a C-terminal transmembrane region. The ge ...
DOI:
10.1016/j.gene.2019.144287

94. A full-scale study of high-rate anaerobic bioreactors for whiskey distillery wastewater treatment with size fractionation and metagenomic analysis of granular sludge

Author:
Justin Chun-Te Lin; Yi-Sung Liu; Wei-Kuang Wang
Source:
Bioresource technology 2020 v.306 pp. 123032
ISSN:
0960-8524
Subject:
Bacteroidetes; Methanosaeta; Nitrospiraceae; alkalinity; anaerobic digesters; bacteria; chemical oxygen demand; distillery effluents; fractionation; gas chromatography-mass spectrometry; high-throughput nucleotide sequencing; metagenomics; pH; sludge; urea; urethane; wastewater treatment; whisky
Abstract:
... Two full-scale high-rate bioreactors, i.e. external circulation sludge bed (ECSB) and expanded granular sludge bed (EGSB), were monitored for three years. Their performances for treating wastewater in a whiskey distillery were compared in terms of COD, pH, alkalinity and VFA. Even though feed flowrate highly fluctuated, COD removals of ECSB and EGSB were both excellent (95.7 ± 1.3% and 94.8 ± 3.0% ...
DOI:
10.1016/j.biortech.2020.123032

95. A fully integrated bacterial pathogen detection system based on count-on-a-cartridge platform for rapid, ultrasensitive, highly accurate and culture-free assay

Author:
Won-Il Lee; Younghyeon Park; Sajal Shrivastava; Taekeon Jung; Montri Meeseepong; Jaelin Lee; Byeungwoo Jeon; Sung Yang; Nae-Eung Lee
Source:
Biosensors & bioelectronics 2020 v.152 pp. 112007
ISSN:
0956-5663
Subject:
Staphylococcus aureus; algorithms; bacteria; biosensors; fluorescence; food matrix; human diseases; magnetism; microbial detection; microbial load; microbiological criteria; monitoring; plate count; point-of-care testing; virulent strains
Abstract:
... Rapid, sensitive and accurate point-of-care-testing (POCT) of bacterial load from a variety of samples can help prevent human infections caused by pathogenic bacteria and mitigate their spreading. However, there is an unmet demand for a POCT device that can detect extremely low concentrations of bacteria in raw samples. Herein, we introduce the ‘count-on-a-cartridge’ (COC) platform for quantitatio ...
DOI:
10.1016/j.bios.2020.112007

96. A genomic integration platform for heterologous cargo encapsulation in 1,2-propanediol utilization bacterial microcompartments

Author:
Taylor M. Nichols; Nolan W. Kennedy; Danielle Tullman-Ercek
Source:
Biochemical engineering journal 2020 v.156 pp. 107496
ISSN:
1369-703X
Subject:
Salmonella Typhimurium; bacteria; biochemical pathways; encapsulation; enzymes; fluorescence; genomics; loci; metabolic engineering; operon; signal peptide; toxicity
Abstract:
... Bacterial microcompartments (MCPs) are protein structures that encapsulate specific metabolic pathways in bacteria. The 1,2-propanediol utilization (Pdu) MCP in Salmonella enterica serovar Typhimurium LT2 encapsulates the pathway for 1,2-propanediol degradation to sequester a toxic intermediate, enable cofactor recycling, and enhance pathway flux. The Pdu MCP has potential as an enclosed scaffold ...
DOI:
10.1016/j.bej.2020.107496

97. A hemoglobin-derived antimicrobial peptide, LCH4, from the large yellow croaker (Larimichthys crocea) with potential use as a food preservative

Author:
Shen Yang; Yuting Dong; Jude Juventus Aweya; Jian Li; Xinyi Chen; Yueling Zhang; Guang-Ming Liu
Source:
Lebensmittel-Wissenschaft + Technologie 2020 v.131 pp. 109656
ISSN:
0023-6438
Subject:
Larimichthys crocea; Staphylococcus aureus; Vibrio parahaemolyticus; antimicrobial peptides; antimicrobial properties; bacteria; biofilm; bioinformatics; cell membranes; cell viability; cell walls; cytotoxicity; food preservatives; hemoglobin; hepatocytes; humans; mechanism of action; minimum inhibitory concentration; permeability; solutes; soy sauce; survival rate; transmission electron microscopy; virulent strains
Abstract:
... In this study, bioinformatics tools were used to predict potential antimicrobial peptides according to the hemoglobin sequence of the large yellow croaker (Larimichthys crocea). A novel antimicrobial peptide, designated as LCH4, was identified, and its antimicrobial effect and mechanism of action on Staphylococcus aureus and Vibrio parahaemolyticus were explored. The minimal inhibitory concentrati ...
DOI:
10.1016/j.lwt.2020.109656

98. A high amylase‐producing bacterial strain exhibiting the phosphorus‐dissolving ability, isolated from freshwater aquaculture pond

Author:
Qinglin Yang; Lijun Luo; Yanhong Jiang; Jiaojiao Zhang; Xiaoqi Tang; Mi Jiang; Zhengli Wu; Yanhong Li
Source:
Aquaculture research 2020 v.51 no.10 pp. 4315-4326
ISSN:
1355-557X
Subject:
Bacillus cereus; Ctenopharyngodon idella; aluminum; amylases; aquaculture systems; bacteria; calcium; culture media; enzyme activity; fermentation; fish; freshwater aquaculture; hydrolysis; inoculum; iron; magnesium; manganese; nucleotide sequences; pH; peptones; phosphorus; probiotics; ribosomal DNA; sequence analysis; starch; temperature; wastes; water quality
Abstract:
... Amylase‐producing bacteria could improve water quality contaminated by waste from feed residue and fish metabolism, thereby increasing the efficiency of aquaculture systems. The objective of this research was to screen and optimize fermentation conditions of a high amylase‐producing strain. Four amylase‐producing bacterial strains (named S458‐1, G05, H38 and B09) were isolated from a grass carp po ...
DOI:
10.1111/are.14775

99. A high-efficiency and eco-friendly degumming process for ramie fibers

Author:
Tong Shu; Yun Bai; Ya-wei Wang; Hui-hui Wang; Pan-deng Li; Meng-xiong Xiang; Tian-yi Yu; Hang Xu; Long-jiang Yu
Source:
Journal of cleaner production 2020 v.276 pp. 124217
ISSN:
0959-6526
Subject:
Fourier transform infrared spectroscopy; Pectobacterium carotovorum; bacteria; beta-mannosidase; community structure; degumming; enzyme activity; fabrics; genes; polygalacturonase; ramie; scanning electron microscopy; sequence analysis; temperature; textile industry; xylanases; China
Abstract:
... Degumming is the most critical step in the preparation of ramie fibers for textile application. However, the traditional chemical degumming process, which uses large amounts of alkali at high temperature, has been a severe challenge to the environment. In this study, a bacterial strain Pectobacterium carotovorum HG-49, was isolated and successfully applied to the degumming of ramie fibers at an in ...
DOI:
10.1016/j.jclepro.2020.124217

100. A histological and ultrastructural study of equine peripheral caries

Author:
D. Borkent; S. Smith; P. M. Dixon
Source:
Equine veterinary journal 2020 v.52 no.1 pp. 104-111
ISSN:
0425-1644
Subject:
bacteria; electron microscopy; histology; horses; teeth; tissues; veterinary dentistry
Abstract:
... BACKGROUND: Peripheral caries (PC) has recently been shown to affect over half of UK and Australian horses. OBJECTIVES: To examine affected cheek teeth histologically and ultrastructurally, to assess the possible route(s) of carious infection into the dental tissues and to further describe the pathological changes in affected teeth. STUDY DESIGN: Descriptive pathological study. METHODS: Four contr ...
DOI:
10.1111/evj.13134