An official website of the United States government
Here’s how you know
Official websites use .gov
A .gov website belongs to an official government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.
... The quinol oxidation site QD in E. coli respiratory nitrate reductase A (EcNarGHI) reacts with the three isoprenoid quinones naturally synthesized by the bacterium, i.e. ubiquinones (UQ), menaquinones (MK) and demethylmenaquinones (DMK). The binding mode of the demethylmenasemiquinone (DMSK) intermediate to the EcNarGHI QD quinol oxidation site is analyzed in detail using ¹,²H hyperfine (hf) spect ...
... The Asian citrus psyllid Diaphorina citri (Hemiptera: Psylloidea) is a serious pest of citrus species worldwide because it transmits Candidatus Liberibacter spp. (Alphaproteobacteria: Rhizobiales), the causative agents of the incurable citrus disease, huanglongbing or greening disease. Diaphorina citri possesses a specialized organ called a bacteriome, which harbors vertically transmitted intracel ...
... Gut microbiota has a critical role in metabolic diseases, including type 2 diabetes mellitus (T2DM). 3-bromo-4,5-bis(2,3-dibromo-4,5-dihydroxybenzyl)-1,2-benzenediol (BDB) is a natural bromophenol isolated from marine red alga Rhodomela confervoides. Our latest research showed that BDB could alleviate T2DM in diabetic BKS db mice. To find out whether BDB modulates the composition of the gut microb ...
... 2,3-Dihydroxyisovalerate is an intermediate of valine and leucine biosynthesis pathway; however, no natural microorganism has been found yet that can accumulate this compound. Klebsiella pneumoniae is a useful bacterium that can be used as a workhorse for the production of a range of industrially desirable chemicals. Dihydroxy acid dehydratase, encoded by the ilvD gene, catalyzes the reaction of 2 ...
... 3-Nitrooxypropanol (3-NOP) is an investigational compound that acts as an enzyme inhibitor to decrease ruminal methanogenesis. We hypothesized that when feeding 3-NOP to cattle fed a high-forage diet, H₂ would accumulate in the rumen, which could suppress microbial colonization of feed particles and fiber degradation. Therefore, the study investigated the effects of supplementing a high-forage die ...
... Listeria monocytogenes is a Gram-positive facultative anaerobic bacterium that is responsible for the disease, listeriosis. It is particularly lethal in pregnant women, the fetus, the elderly and the immunocompromised. The pathogen survives and replicates over a wide range of temperatures (4 to 42 °C), pH, salt and oxygen concentrations. Because it can withstand various environments,
... The rhamnolipids are a unique class of biosurfactants produced by the bacteria Pseudomonas aeruginosa. These molecules display a high level of surface activity as well as biodegradability. In this study nonionic dirhamnolipid was investigated by utilizing molecular dynamics simulation at the air–water interface as well as in bulk water. Detailed structural analysis is presented for both the interf ...
... Electro-fermentation (EF) is an upcoming technology that can control the metabolism of exoelectrogenic bacteria (i.e., bacteria that transfer electrons using an extracellular mechanism). The fermenter consists of electrodes that act as sink and source for the production and movement of electrons and protons, thus generating electricity and producing valuable products. The conventional process of f ...
... The present study was aimed to get an insight into the bacterial biota of ready-to-eat small crickets (Acheta domesticus) already marketed in the European Union. 16S rRNA gene of the DNAs extracted from thirty-two samples of ready-to-eat crickets commercialized by 4 European Union producers located in Austria, Belgium, France and the Netherlands (2 batches per producer) was analyzed by Polymerase ...
... Rice grain rot disease was detected for the first time in Mazandaran Province, Iran. The bacteria isolated from infected rice plants showed grains rotted and darkening. A Xanthomonas strain closely connected to X. sacchari was identified using molecular and whole genome sequencing approaches confirmed as the causal agent by fulfilling Koch’s postulates. ...
... A wide variety of steroid metabolites synthesized by eukaryotes are all ultimately catabolized by bacteria; while generally saprophytic, pathogenic Mycobacteria have repurposed these pathways to utilize host intracellular cholesterol pools. Steroid degradation is complex, but a recurring theme is that cycles of β-oxidation are used to iteratively remove acetyl- or propanoyl-CoA groups. These β-oxi ...
... Hierarchical 3D gold nano-/microislands (NMIs) are favorably structured for direct and probe-free capture of bacteria in optical and electrochemical sensors. Moreover, their unique plasmonic properties make them a suitable candidate for plasmonic-assisted electrochemical sensors, yet the charge transfer needs to be improved. In the present study, we propose a novel plasmonic-assisted electrochemic ...
... Gardnerella vaginalis (G. vaginalis) is the major bacteria detected in women with bacterial vaginosis (BV). Prevotella bivia (P. bivia) has been demonstrated to show a symbiotic relationship with G. vaginalis. Some men have been shown to be colonized with G. vaginalis in their urogenital or anorectal tracts, however genitourinary infections in males, including balanitis and urethritis, due to this ...
... Poultry is one of the largest sources of animal-based protein in the United States. Poultry processing has grown from a small local network of plants to nearly 500 plants nationwide. Two of the most persistent bacteria in poultry processing are Salmonella and Campylobacter. It was not until the introduction of Hazard Analysis and Critical Control Point systems in 1996 that major efforts to reduce ...
... The bacterium Xanthomonas oryzae pv. Oryzae (Xoo) causes blight in rice worldwide, resulting in significant crop loss. However, no gene underlying a quantitative trait locus (QTL) for resistance against Xoo has been cloned yet. Here, we report the map-based cloning of a QTL, in which the NBS8R gene confers quantitative resistance to Xoo. NBS8R encodes an NB-ARC protein, which is involved in pathog ...
... RNA thermosensors (RNATs), found in the 5′ untranslated region (UTR) of some bacterial messenger RNAs (mRNAs), control the translation of the downstream gene in a temperature-dependent manner. In Listeria monocytogenes, the expression of a key transcription factor, PrfA, is mediated by an RNAT in its 5′ UTR. PrfA functions as a master regulator of virulence in L. monocytogenes, controlling the exp ...
... Bis-(3′–5′)-cyclic dimeric guanosine monophosphate (c-di-GMP) is an important intracellular signaling molecule that affects diverse physiological processes in bacteria. The intracellular levels of c-di-GMP are controlled by proteins acting as diguanylate cyclase (DGC) and phosphodiesterase (PDE) enzymes that synthesize and degrade c-di-GMP, respectively. In the alphaproteobacterium Rhodobacter cap ...
... YajQ, a binding protein of the universal bacterial second messenger cyclic di‐GMP (c‐di‐GMP), affects virulence in several bacterial pathogens, including Xanthomonas campestris. In this bacterium, YajQ interacts with the transcription factor LysR. Upon c‐di‐GMP binding, the whole c‐di‐GMP‐YajQ‐LysR complex is found to dissociate from DNA, resulting in virulence gene regulation. Here, we identify a ...
... Nontypeable strains of Haemophilus influenzae (NTHi) are one of the most common cause of otitis media and the most frequent infection associated with exacerbations of chronic obstructive pulmonary disease; there is currently no vaccine in the U.S. to prevent NTHi. Using bioinformatics and structural vaccinology, we previously identified several NTHi species-conserved and sequence-conserved peptide ...
... BACKGROUND: Novel tools are needed to reduce the nuisance and risk of exotic arbovirus transmission associated with the colonization of temperate regions by Aedes albopictus. The incompatible insect technique (IIT) is a population suppression approach based on cytoplasmic incompatibility between males with manipulated endosymbionts and wild females. Here, we present the results of the first field ...
... A self-immobilization method for microorganisms was developed based on fungal pellets. Generally, pellets have some problems such as cell leakage, cell loading limitation and low mechanical strength. Therefore, biochar was applied to overcome these disadvantages. Atrazine degradable microorganism Arthrobacter sp. ZXY-2 was immobilized by Aspergillus niger Y3 pellets. After adding biochar with opti ...
activated sludge; aerobes; bacteria; biofilm; denitrification; factor analysis; habitats; nitrates; oxygen consumption; polybutylene succinate; sewage; sewage treatment; soil; soil sampling; species abundance; species richness; total nitrogen; zeolites
Abstract:
... Multi-soil-layering (MSL) system with brick-wall pattern structure and gravitational flow can be used for decentralized rural domestic sewage treatment. The capability of soil for contaminant removal is maximized within soil mixture blocks (SMBs). However, the performance of removing nitrate was still not ideal during operation. To improve its performance in MSL system, the relationship between bi ...
Caio F. C. Zamuner; Guilherme Dilarri; Lucia C. Bonci; Luiz L. Saldanha; Franklin Behlau; Tamiris G. S. Marin; Daiane C. Sass; Mauricio Bacci Jr.; Henrique Ferreira
Citrus; European Union; bacteria; chemical analysis; citrus canker; citrus fruits; control methods; copper; corrosion; decontamination; equipment; eugenol; food contamination; fruit quality; growers; isomers; laws and regulations; leafminers; organic matter; plant pathology; population; raw fruit; sodium hypochlorite; trees; tropical plants; windbreaks; Brazil
Abstract:
... According to the most recent regulation, published in 2018, areas or states of Brazil where citrus canker is endemic are no longer obliged to eradicate citrus trees affected by the disease as in the past 60 years. Instead, growers have to adopt a set of control measures, such as copper sprays, windbreaks, and control of the citrus leaf miner to minimize the impact of the disease on fruit quality a ...
... Pumpkin seed is being used traditionally for curing and protecting various ailments. The present study was designed to evaluate the anti-inflammatory, anti-oxidant and anti-bacterial activities of ethanol extract of pumpkin (Cucurbita maxima Linn.) seed oil from hybrid and indigenous varieties. An anti-inflammatory, anti-oxidant, and anti-bacterial activities were assessed by BSA (bovine serum alb ...
... Isolation and fast detection of Clostridium perfringens is essential in veterinary medical diagnostics and veterinary research, as it allows to recommend suitable treatment options after antimicrobial resistance determination, and is essential to study pathogenesis. In this study four selective media were tested for the enumeration of, and selectivity towards C. perfringens in faecal samples from ...
bacteria; calcite; chemical reactions; effective porosity; hydraulic conductivity; laboratory experimentation; models; permeability; soil mechanical properties; urease; water resources
Abstract:
... Microbially induced calcite precipitation (MICP) provides the potential for developing innovative and environmentally friendly techniques to improve the engineering properties of soil through reduction of permeability in soil and increase of soil stiffness and strength. In the present research work, coupled bio-chemo-hydraulic modelling was developed to enhance the understanding of the coupled pro ...
... The human gut contains trillions of microorganisms with a great diversity that are associated with various health benefits. Recent studies have reported an increasing correlation between diet, gut microbiota, and human health, indicating rapid development in the field of gut health. Diet is an important factor that determines the gut microbiota composition. The gut comprises great diversities of m ...
... The objective of this cross-sectional study was to investigate prevalence, causative udder pathogens and their antimicrobial resistance (AMR), as well as cow and herd risk factors associated with subclinical mastitis (SCM = cows with at least one udder quarter with california mastitis test (CMT) score > 2) and intramammary infections (IMI) caused by Staphylococcus(S.) aureus or Non aureus staphylo ...
... The GluM gene (1491-bp) coding for a β-glucosidase comprising a single catalytic glycoside hydrolase family 1 domain from an earthworm (Eisenia fetida)-symbiotic bacterium, Cellulosimicrobium funkei HY-13, was cloned and over-expressed in Escherichia coli BL21. The recombinant histidine-tagged enzyme (rGluM: 56 kDa) displayed the highest cleavage activity toward p-nitrophenyl (pNP)-β-d-glucopyrano ...
... In this study, we present a disposable and inexpensive paper-like gold nanoparticle-embedded cellulose nanofibril substrate for the rapid enumeration of Escherichia coli (E. coli) using surface-enhanced Raman scattering (SERS) mapping. A disposable SERS substrate was simply constructed by mixing CNF and gold chloride solution at 120 °C in a water bath. The application of the resulting substrate wa ...
... Biomaterial-associated bacterial infection is one of the major causes of implant failure. The treatment of such an implant infection typically requires the elimination of bacteria and acceleration of tissue regeneration around implants simultaneously. To address this issue, an ideal implanted material should have the dual functions of bacterial infection therapy and tissue regeneration at the same ...
alkalinity; bacteria; bacterial infections; cell proliferation; ceramics; cytotoxicity; electron transfer; electron transport chain; magnesium; magnesium oxide; osteoblasts; oxidative stress; polymers; titanium; zirconium oxide
Abstract:
... Multifarious strategies have been proposed to enhance the antibacterial ability of implant surfaces for preventing bacterial infection, however, developing facile and universal modification methods still remains extremely elusive. Herein, inspired by the fact that the electron transfer respiratory chain of bacteria is embedded in the membrane, we proposed a novel strategy of local alkalinity distu ...
... Cetyltrimethylammonium bromide (CTAB), a cationic surfactant, is known to have strong bactericidal potential. In this study, we report a facile approach for preparing CTAB-containing polysulfone-sulfonated polyethersulfone (PSF-SPES) based ultrafiltration membranes with antibacterial properties. The CTAB was added in gelation medium at three different concentrations and made an electrostatic inter ...
... Production of urease enzyme from ureolytic bacteria (e.g. Sporosarcina pasteurii) is essential for precipitation of CaCO₃. This bioprocess is notably utilized for soil improvement, biocementation and bioremediation of heavy metals. Despite the viability for field-scale implementation as a suitable alternative to conventional treatment methods, most reports in the literature are constrained to labo ...
... Fibrinogen-related proteins (FREPs) are widely found in both vertebrates as well as invertebrates, and they play a crucial role in host immunity. In this study, we isolated a novel ficolin gene (Mnfico3) from the oriental river prawn Macrobrachium nipponense. The complete cDNA sequence of Mnfico3 was 1133 bp long, containing an open reading frame of 765 bp coding for Mnfico3, a protein consisting ...
... Interleukin-3 (IL-3), IL-5, and granulocyte-macrophage colony-stimulating factor (GM-CSF) are related cytokines that signal through receptors possessing the β common (βc) chain. As a family, these cytokines combine rather non-specific hematopoietic growth factor properties with a special importance for eosinophils, basophils, and mast cells. In fish the cytokines of this family are called IL-5fam, ...
... Fusobacterium nucleatum. nucleatum (Fn.n) is associated with colorectal carcinoma. A highly sensitive fluorescence quenching-recovery detection platform based on rolling circle amplification and hairpin molecular beacon technology for the specific analysis of Fn.n, hairpin MB being used to achieve double quenching. First, a specific recognition sequence target in the padlock probe was designed bas ...
... Bacterial microcompartments (MCPs) are protein structures that encapsulate specific metabolic pathways in bacteria. The 1,2-propanediol utilization (Pdu) MCP in Salmonella enterica serovar Typhimurium LT2 encapsulates the pathway for 1,2-propanediol degradation to sequester a toxic intermediate, enable cofactor recycling, and enhance pathway flux. The Pdu MCP has potential as an enclosed scaffold ...
Fourier transform infrared spectroscopy; Pectobacterium carotovorum; bacteria; beta-mannosidase; community structure; degumming; enzyme activity; fabrics; genes; polygalacturonase; ramie; scanning electron microscopy; sequence analysis; temperature; textile industry; xylanases; China
Abstract:
... Degumming is the most critical step in the preparation of ramie fibers for textile application. However, the traditional chemical degumming process, which uses large amounts of alkali at high temperature, has been a severe challenge to the environment. In this study, a bacterial strain Pectobacterium carotovorum HG-49, was isolated and successfully applied to the degumming of ramie fibers at an in ...
bacteria; electron microscopy; histology; horses; teeth; tissues; veterinary dentistry
Abstract:
... BACKGROUND: Peripheral caries (PC) has recently been shown to affect over half of UK and Australian horses. OBJECTIVES: To examine affected cheek teeth histologically and ultrastructurally, to assess the possible route(s) of carious infection into the dental tissues and to further describe the pathological changes in affected teeth. STUDY DESIGN: Descriptive pathological study. METHODS: Four contr ...
... In the summer of 2010, a sustained multispecies fish kill, affecting primarily adult red drum (Sciaenops ocellatus) and Atlantic stingray (Dasyatis sabina), along with various baitfish such as menhaden (Brevoortia spp.) and shad (Dorosoma spp.), was documented for six weeks along 50 km of the Lower St. Johns River (LSJR), Florida. An Aphanizomenon flos-aquae bloom was present in the freshwater rea ...
... Nucleic acid-based detection methods are accurate and rapid, which are widely-used in food-borne pathogen detection. However, traditional nucleic acid-based detection methods usually rely on special instruments, weakening their practicality for on-site tests in resource-limited locations. In this work, we developed a convenient and affordable method for food-borne pathogen detection based on a lat ...
... Mathematical models represent essential tools allowing a quantitative analysis of an epidemic system with the consequent identification of possible strategies to control a disease outbreak or even to prevent it. However, to be used in decision-making, they must be carefully parametrized and validated with epidemiological data as well as biological information on the relevant players. Here, benefit ...
... BACKGROUND: Although, peripheral caries (PC) affects almost half of UK horses, no comprehensive microbiological study has been performed on this disorder. As a high proportion of oral bacteria cannot be conventionally cultured, molecular microbiological techniques such as Next Generation Sequencing are required to examine the complex oral bacteria community. OBJECTIVES: To identify the microbiota ...
Escherichia coli; Soil and Water Assessment Tool model; bacteria; monitoring; pH; statistics; water quality; watersheds
Abstract:
... Quantifying bacteria fluxes and contaminants from the point and nonpoint sources in a watershed are important for the management of water quality and safeguard public health. Therefore, the appropriate characterization of bacteria from different sources is necessary for understanding of fate and transport of bacteria in watersheds. However, it is challenging to simulate the effects of pH on bacter ...
John Sanders; Yue Xie; David Gazzola; Hanchen Li; Ambily Abraham; Kelly Flanagan; Florentina Rus; Melanie Miller; Yan Hu; Sierra Guynn; Austin Draper; Sridhar Vakalapudi; Katherine H. Petersson; Dante Zarlenga; Robert W. Li; Joseph F. Urban; Gary R. Ostroff; Anne Zajac; Raffi V. Aroian
... Haemonchus contortus is a critical parasite of goats and sheep. Infection by this blood-feeding gastrointestinal nematode (GIN) parasite has significant health consequences, especially in lambs and kids. The parasite has developed resistance to virtually all known classes of small molecule anthelmintics used to treat it, giving rise in some areas to multidrug resistant parasites that are very diff ...
... Chemical pesticides or insecticides with complex structures are highly abundant in the biosphere and have inevitable side effects on farmland, natural resources, and human health. Deltamethrin is the most popular and widely used pesticide that disrupts the cellular calcium channels. In the present study, isolated strains of bacteria were examined to determine the ones that were capable of degradin ...
... A facile immunosensor was constructed based on a barometer indicator using a double-antibody- sandwich method for rapid and sensitive detection of Escherichia coli (E. coli) from water samples. At the present study, Anti- E. coli DH-5 polyclonal antibody was modified through enriching carboxylated magnetic beads and catalase functionalized gold nanoparticles (AuNPs)- loaded nanospheres. The functi ...
... A large number of bioactive peptides derived from breast milk have been identified to be multifunctional having anti-inflammatory, immunoregulatory and antimicrobial activities. Here, we report that an endogenous peptide located at β-casein 211-225 amino acid from human breast milk (hereafter called CAMP₂₁₁₋₂₂₅) presents specific antimicrobial activity against pathogenic E. coli and Y. enterocolit ...
... A previously unknown haplotype of the plant pathogen ‘Candidatus Liberibacter solanacearum’ (Lso) was found in cultivated carrots and parsnips in eastern Finland. That same haplotype was found in western Finland, over 300 km away, in the family Polygonaceae, the species Fallopia convolvulus (wild buckwheat) and Persicaria lapathifolia (pale persicaria) growing as weeds within carrot and parsnip fi ...
... To establish a simple and rapid high-resolution melting curve (HRM) method, 5 different strains of Salmonella were identified by adding DNA denaturants at different concentrations into the HRM system to change the characteristics of DNA melting and to obtain different Tm (dissolving temperature) values of DNA from different target bacteria. When the concentration of n-butanol was 7% (v/v), the Tm ...
... Nisin is an antimicrobial peptide with bacterial, fungicidal, virucidal properties, attacking bacteria and destroying the cell membranes. Thanks to its stability to hard conditions, it is a candidate for the use as molecular recognition elements in biosensing platform. In this work, the use of nisin as a biological molecule for the development of a sensitive biosensor for bacteria detection is rep ...
Escherichia coli; bacteria; biotechnology; computer simulation; detergents; enzyme activity; genomic libraries; metagenomics; molecular weight; pH; phylogeny; proteolysis; salt tolerance; sequence analysis; serine proteinases; temperature; thermal stability; East China Sea
Abstract:
... Thermal activity and stability are important characteristics for proteases applied in the detergent, pharmaceutical, food, and other green industries. With the intent to discover thermostable novel proteases, we constructed a fosmid metagenomic library from marine sediments in the East China Sea and isolated a clone endowed with high proteolytic activity from this library. Sequence analysis of the ...
... Screening for anti-anaerobic drug candidates is still challenging although the anaerobic bacteria are important sources for human infections, because the method for anti-anaerobic activity testing is not readily available with low-cost and -expertise. We report a novel method for the determination of the anti-anaerobic activity of drug candidates by automated headspace-gas chromatography (HS-GC). ...
... The obligate intracellular bacterium, Chlamydia pneumoniae, has been identified as a risk factor for several chronic inflammatory diseases in addition to respiratory tract infections. The dissemination of C. pneumoniae from respiratory tract to secondary sites of infection occurs via infected monocyte / macrophage line cells, in which C. pneumoniae can persist as an antibiotic-refractory phenotype ...
... Marine mollusk aquaculture has more than doubled over the past twenty years, accounting for over 15% of total aquaculture production in 2016. Infectious disease is one of the main limiting factors to the development of mollusk aquaculture, and the difficulties inherent to combating pathogens through antibiotic therapies or disinfection have led to extensive research on host defense mechanisms and ...
... Innate immune response is insisted upon detection of foreign intracellular DNA or RNA derived from viruses and bacteria. This reaction is important to initiate an effective protective response for the host cells. This crucial step is induced by cytosolic nucleic acids sensors/binding proteins, which triggers the production of type I or type III interferons (IFNs) and proinflammatory cytokines such ...
antibiotic resistance; antibiotic resistance genes; antibiotics; bacteria; chemical compounds; constructed wetlands; ecosystems; environmental impact; flame retardants; hormones; humans; personal care products; pesticides; plasticizers; surfactants; wastewater; wastewater treatment
Abstract:
... Emerging organic contaminants (EOCs) include a diverse group of chemical compounds, such as pharmaceuticals and personal care products (PPCPs), pesticides, hormones, surfactants, flame retardants and plasticizers. Many of these compounds are not significantly removed in conventional wastewater treatment plants and are discharged to the environment, presenting an increasing threat to both humans an ...
bacteria; cost effectiveness; dyes; flocculants; flocculation; heavy metals; industrialization; nanoparticles; physicochemical properties; water pollution; water treatment
Abstract:
... In recent years, the development of nanoparticle materials for water treatment has received great attention. From an industrial technological view point, the application of nanomaterials in the twenty-first century for water treatment will be the focal point of advanced materials design, processing and progress. In this context, the potential utilisation of different types of flocculants to clean ...
Christian Sonne; Jan Lakemeyer; Jean-Pierre Desforges; Igor Eulaers; Sara Persson; Iben Stokholm; Anders Galatius; Stephanie Gross; Katharina Gonnsen; Kristina Lehnert; Emilie U. Andersen-Ranberg; Morten Tange Olsen; Rune Dietz; Ursula Siebert
... Here we review the state-of-the-art of pathogens in select marine and terrestrial key species of the Baltic Sea, i.e. ringed seal (Pusa hispida), harbour seal (Phoca vitulina), grey seal (Halichoerus grypus), harbour porpoise (Phocoena phocoena), common eider (Somateria mollissima), pink-footed goose (Anser brachyrhynchus) and white-tailed eagle (Haliaeetus albicilla). This review is the first to ...
... Brucella is a facultative intracellular bacterium, dividing into smooth- and rough-type Brucella. Smooth-type Brucella can dissociate into rough mutants with cytotoxicity for macrophages during infection, which is critical for Brucella egress and dissemination. However, the mechanism of cytotoxicity infected by rough Brucella is incomplete. In this study, we verified that a rough-type Brucella (RB ...
... A sufficiently fast and simple antimicrobial susceptibility testing (AST) is urgently required to guide effective antibiotic usages and to surveil the antimicrobial resistance rate. Here, we establish a rapid, quantitative, and high-throughput phenotypic AST by measuring electrons transferred from the interiors of microbial cells to external electrodes. Because the transferred electrons are based ...
... Rapid and sensitive detection of foodborne pathogens in food industry is of high importance in day-to-day practice to ensure safe food. To address this issue, multiple foodborne pathogens are targeted for rapid identification based in DNA amplification. A 3D PDMS sponge was fabricated using salt crystals as scarifying mold and functionalized with a ligand, apolipoprotein-H (ApoH), to test bacteria ...
... Complement is a key first line innate host defense system in the blood of vertebrates. Upon activation, this powerful defense mechanism can elicit inflammatory responses, lyse non-self-cells, or mark them for opsonophagocytic removal. Blood-feeding arthropods thus require the ability to block host complement activation in the bloodmeal to prevent undesired cell or tissue damage during feeding. The ...
... Anaerobic ammonium oxidation (anammox), denitrifying anaerobic methane oxidation bacteria (DAMO) have received great attention for their excellent performance in nitrogen removal. However, not much study focused on the co-existence of anammox, DAMO, and denitrification in constructed wetlands, not to mention the advantage of their application in mitigating the necessary byproduct nitrous oxide (N₂ ...
Paracoccus marcusii; bacteria; equations; galactooligosaccharides; galactose; hydrolysis; lactose; models; response surface methodology; statistical analysis; temperature; yeast extract; Bay of Bengal
Abstract:
... A new β-galactosidase-producing bacterium KGP, isolated from the Bay of Bengal, was identified as Paracoccus marcusii through morphology, biochemistry and 16S rRNA sequencing. This study is the first report on the production of β-galactosidase from P. marcusii. The medium components for the high yield of β-galactosidase were optimised using response surface methodology (RSM). A set of 17 experimen ...
... The first comprehensive timetree is presented for phytoplasmas, a diverse group of obligate intracellular bacteria restricted to phloem sieve elements of vascular plants and tissues of their hemipteran insect vectors. Maximum likelihood-based phylogenetic analysis of DNA sequence data from the 16S rRNA and methionine aminopeptidase (map) genes yielded well resolved estimates of phylogenetic relati ...
Clostridium; Escherichia coli; anorexia; bacteria; blood serum; calves; cohort studies; creatinine; disease course; enzyme activity; gamma-glutamyltransferase; hypoproteinemia; medical records; mortality; necropsy; neutrophils; prognosis; signs and symptoms (animals and humans); statistical analysis; telephones; veterinary medicine
Abstract:
... BACKGROUND: Abomasitis is a syndrome affecting young milk‐fed calves. The current veterinary literature describes mainly its necropsy findings. OBJECTIVES: To describe the clinical presentation, complementary tests, treatments, and case‐fatality rate of calves with a clinical diagnosis of abomasitis and to identify potential factors associated with outcome. METHODS: Observational retrospective coh ...
... Dermiyati, Suharjo R, Telaumbanua M, Yosita R, Sari AW, Andayani AP. 2020. Abundance and characterization of microorganisms isolated from oil palm empty fruit bunches waste under aerobic, anaerobic, and facultative anaerobic conditions. Biodiversitas 21: 4213-4220. Increased production of palm oil causes an increase in oil palm empty fruit bunches waste, while the utilization of the waste has not ...
... Acacia longifolia is a worldwide invader that cause damage in ecosystems, expanding largely after wildfires, which promote germination of a massive seed bank. As a legume, symbiosis is determinant for adaptation. Our study aims to isolate a wider consortium of bacteria harboured in nodules, including both nitrogen and non-nitrogen fixers. Furthermore, we aim to evaluate the effects of fire in nodu ...
... Phenolic root exudates (PREs) released from plants are potentially effective for the degradation of aromatic compounds, yet the inherent process and mechanism remain insufficiently elucidated. In this study, we selected four representative PREs of P. australis, namely p-hydroxybenzoic acid (PHA), p-coumaric acid (PCA), caffeic acid (CA), and ferulic acid (FA), to investigate the accelerated biodeg ...
... Infectious diseases have become one of the most threatening global challenge with high morbidity and mortality, bringing great difficulties to clinical diagnosis and treatment. New strategy for high-specific and sensitive bacteria detection are urgently needed in facing the crisis of worldwide antibiotic resistance. Herein, a novel method through the integration of dual aptamer technology and CRIS ...
... We describe two novel species of Acholeplasma sp. strain N93 and Mycoplasma sp. strain LR5794 which were isolated from the nasopharynx of a horse from the United Kingdom and from the oral cavity of a North American raccoon from Canada, respectively. These strains were phenotypically and genetically characterized and compared to other established Mycoplasma and Acholeplasma species. Both strains ar ...
Larimichthys crocea; Pseudomonas plecoglossicida; bacteria; bioinformatics; fish; fish diseases; granuloma; horizontal gene transfer; kidneys; liver; models; multigene family; mutants; pathogenesis; pathogens; spleen; type VI secretion system; virulence; virulent strains
Abstract:
... Pseudomonas plecoglossicida is one of the most prevalent pathogens affecting the large yellow croaker (LYC; Larimichthys crocea), causing a disease characterized by granuloma formation in the spleen, kidney and liver. Previously we discovered two active type VI secretion systems (T6SSs) in P. plecoglossicida XSDHY-P (a fish pathogenic strain). However, whether these systems are involved in bacteri ...
... Cortisol is a key modulator of the immune response in fish, and considered an essential molecule for adaptation of animals to stress and overcoming the stressor. Antagonist effects, pro and anti-inflammatory have been associated to acute increases in cortisol levels, however knowledge about the mechanisms of this regulation in tropical teleosts is scarce. The effects of pharmacological inhibition ...
... Coupling of methanogenesis and denitrification with elevated nitrate under the methanogenic condition shows potential advantages for (N-) heterocyclic compounds removal and methane recovery simultaneously. However, methane production, sludge characteristics and microbial evolution response to elevated nitrate were seldom reported. Here, an experiment lasing about 300 days under the methanogenic co ...
Streptomyces; antibiotics; bacteria; biosynthesis; chemical reactions; chemical structure; coenzyme A; cysteamine; drugs; enzymes; inhibitory concentration 50; virulent strains
Abstract:
... Phosphopantetheine is a key structural element in biological acyl transfer reactions found embedded within coenzyme A (CoA). Phosphopantothenoylcysteine synthetase (PPCS) is responsible for installing a cysteamine group within phosphopantetheine. Therefore, it holds considerable potential as a drug target for developing new antimicrobials. In this study, we adapted a biochemical assay specific for ...
... Successful vaccination, especially with safe vaccines such as component/subunit vaccines, requires proper activation of innate immunity and, for this purpose, adjuvant is used. For clinical use, alum is frequently used while, for experimental use, CFA, containing Mycobacterial components, was often used. In this report, we demonstrated that mycolic acids (MA), major and essential lipid components ...
... BACKGROUND: Anthocyanins and flavonols play a significant role in contributing to wine color and mouthfeel, and the interaction of malolactic fermentation with these compounds is not well known. Here we investigated the adsorption of these compounds by Oenococcus oeni and Lactobacillus plantarum. RESULTS: Delphinidin‐3‐glucoside (D3G) was adsorbed the most, followed by malvidin‐3‐glucoside (M3G) a ...
... Nonalcoholic fatty liver disease (NAFLD) is the most common chronic liver disease in Western countries, and the gut-liver axis is implicated in liver disease pathogenesis. We hypothesize that advanced liver steatosis accompanies an increase in hepatic inflammation, colonic secondary bile acids (BAs) and secondary BA-producing bacteria in mice fed a high-fat (HF) diet model of obesity. Four-week ol ...
... Foodborne diseases caused by pathogens are great threats to human health. Rapid detection of foodborne pathogens at an early stage is imperative for preventing the outbreak of diseases. The identification and detection of foodborne pathogens by biosensors have attracted great attention due to their high sensitivity, nearly real-time quantification without enrichment and the possibility of on-site ...
... The production of biodegradable plastics is considered to be a way to reduce plastic waste issue. Among others, oxo-degradant additives enable a faster degradation of plastics in the environment. However, the introduction of these new materials could provoke the release of substances potentially toxic in the environment. This work determined and compared the toxicity of leachates from various addi ...
... A Gram-stain-negative, rod-shaped, obligately aerobic, nonflagellated, and chemoheterotrophic bacterium, designated IMCC3088ᵀ, was isolated from coastal seawater of the Yellow Sea. The 16S rRNA gene sequence analysis indicated that this strain belonged to the family Halieaceae which shared the highest sequence similarities with Luminiphilus syltensis NOR5-1Bᵀ (94.5%) and Halioglobus pacificus S1-7 ...
Blaise Fleury; Eldho Abraham; Joshua A. De La Cruz; Varun S. Chandrasekar; Bohdan Senyuk; Qingkun Liu; Vladyslav Cherpak; Sungoh Park; Jan Bart ten Hove; Ivan I. Smalyukh
... Improving building energy performance requires the development of new highly insulative materials. An affordable retrofitting solution comprising a thin film could improve the resistance to heat flow in both residential and commercial buildings and reduce overall energy consumption. Here, we propose cellulose aerogel films formed from pellicles produced by the bacteria Gluconacetobacter hansenii a ...
... Hollow mesoporous silica microsphere (HMSM) particles are one of the most promising vehicles for efficient drug delivery owing to their large hollow interior cavity for drug loading and the permeable mesoporous shell for controlled drug release. Here, we report an easily controllable aerosol-based approach to produce HMSM particles by continuous spray-drying of colloidal silica nanoparticles and E ...
... Bacillus bacteria have major utility in large-scale production of industrial enzymes, among which proteases have particular importance. B. subtilis B22, an aerobic and chemotrophic strain, was isolated from kimchi and identified by 16S rRNA gene sequencing. Extracellular protease production was determined in basic medium, with 1% (w/v) casein as substrate, by submerged fermentation at 37 °C under ...
Andrea P. Castellanos-Arévalo; Andrés A. Estrada-Luna; José L. Cabrera-Ponce; Eliana Valencia-Lozano; Humberto Herrera-Ubaldo; Stefan de Folter; Alejandro Blanco-Labra; John P. Délano-Frier
... KEY MESSAGE: Transgenic A. hypochondriacus and A. hybridus roots were generated. Further, a distinct plant regeneration program via somatic embryos produced from hairy roots was established. Work was implemented to develop an optimized protocol for root genetic transformation of the three grain amaranth species and A. hybridus, their presumed ancestor. Transformation efficiency was species-specifi ...
... Ferritins are a large family of iron storage proteins, which are used by bacteria and other organisms to avoid iron toxicity and as a safe iron source in the cytosol. Agrobacterium tumefaciens, a phytopathogen, has two ferritin‐encoding genes: atu2771 and atu2477. Atu2771 is annotated as a Bfr‐encoding gene (Bacterioferritin, Bfr) and atu2477 as a Dps‐encoding gene (DNA binding protein from starve ...
... The main nitrogen (N) sources in soybean production originate from soil fixing bacteria Bradyrhizobium spp. and from mineralization of soil organic N. These sources of N are often not sufficient to cover the N needs of the soybean. The present two-year field study aimed to evaluate the effects of soybean genotypes (Valjevka and Galina) and rates of starter fertilizer N (0, 30, 60, and 90 kg ha⁻¹) ...
alkali treatment; bacteria; coatings; electromagnetic interference; energy conversion; fabrics; heat; heaters; humans; hydrogen bonding; permeability; polymers; solar radiation; temperature; thermotherapy
Abstract:
... Multifunctional, high-performance wearable heaters are highly desired for future human health-related applications but are generally hindered by the absence of flexibility, air-permeability, and clothes-knittability. Here, polymeric textile-based wearable heaters are constructed by decorating an MXene on the fiber surface via a simple solution dip coating technique. Alkali pretreatment of textiles ...
Cyanobacteria; Pseudomonadaceae; airborne microorganisms; alpha-Proteobacteria; antibiotic resistance genes; bacteria; bacterial communities; community structure; health effects assessments; monitoring; particulates; physicochemical properties; public health; rain; rain intensity; relative humidity; risk assessment; statistical analysis; temperature; variance; washing; China
Abstract:
... The biotoxicity and public health effects of airborne bacteria and antibiotic resistance genes (ARGs) in fine particulate matter (PM₂.₅) are being increasingly recognized. The characteristics of bacterial community composition and ARGs in PM₂.₅ under different rainfall conditions were studied based on the on-site synchronous measurements in downtown Beijing. Marked differences were evident in the ...
... Akkermansia muciniphila is a mucin-degrading bacterium that has shown the potential to provide anti-inflammatory and anti-obesity effects in mouse and man. We here focus on companion animals, specifically cats and dogs, and evaluate the microbial degradation of mucus and its health impact in the context of the worldwide epidemic of pet obesity. A literature survey revealed that the two presently k ...
... Acinetobacter baumannii (A. baumannii) is a common opportunistic nosocomial pathogen, which is able to produce biofilms on the surface of indwelling medical devices, and consequentially causes severe infections in clinical settings. In order to identify genes that involved in the biofilm formation of A. baumannii, the differential expression of genes between biofilms and planktonic cells was analy ...
... Cystic fibrosis (CF), the most common autosomal-recessive genetic disease in the Caucasian population, is characterized by frequent respiratory infections and progressive lung disease. Fungal species are commonly found in patients with CF, and among them, Aspergillus fumigatus is the most frequently isolated. While bacteria, particularly Pseudomonas aeruginosa, have a well-established negative eff ...
... Soils may be contaminated by oily wastewater during both upstream and downstream oil and gas production processes such as extraction, transportation, and refining. Oil-contaminated sites could nonetheless be used for agricultural purposes in arid regions provided they are managed appropriately. The present study introduces the successful application of a novel nitrogen-fixing, aromatic hydrocarbon ...
... The correlation between the allocation of trisoxazole macrolides in the capitums, appendages, and bases of the sponge Penares cf. nux and the surface-attached bacteria on the corresponding parts was examined. The kabiramide contents were highest in the capitums, followed by the appendages and bases. Conversely, direct counts of cultivable surface-attached bacteria showed that the bacteria aggregat ...
bacteria; biological control; composts; enzymes; fungi; genes; green waste; microbial communities; niches; plant pathogens; restriction fragment length polymorphism; ribosomal RNA; species diversity
Abstract:
... Green waste composts are obtained from agricultural production chains; their suppressive properties are increasingly being developed as a promising biological control option in the management of soil-borne phytopathogens. The wide variety of microbes harbored in the compost ecological niches may regulate suppressive functions through not yet fully known underlying mechanisms. This study investigat ...
Charlotte Holme Nielsen; Yan Hui; Duc Ninh Nguyen; Agnethe May Ahnfeldt; Douglas G. Burrin; Bolette Hartmann; Anne Birgitte Heckmann; Per Torp Sangild; Thomas Thymann; Stine Brandt Bering
amino acids; animal models; bacteria; brain; breast milk; cognition; colon; cortisol; endpoints; enzyme activity; immunity; lactalbumin; metabolites; microvilli; milk; mineral content; neonatal development; neutrophils; nutrients; swine; tissues; whey protein concentrate
Abstract:
... Human milk is rich in nutritional factors, such as alpha-lactalbumin (α-Lac), and important for neonatal development, but nutrient supplementation may be required for optimal growth. Using a pig model, we hypothesized that α-Lac-enriched whey protein concentrate (WPC) supplementation improves neonatal development. Cesarean-delivered preterm pigs were fed either dilute bovine milk (REF) or REF milk ...
... The evolution of multidrug resistance in bacteria instigates the decline in effectiveness of antibiotics. Nanoparticle-enzyme bioconjugates are a recent interest in countering biofilm-related infections due to their stability and targeted delivery. The synergistic effect of α-amylase and biosynthesized silver nanoparticles (AgNPs) towards biofilms remains less explored. In this study, we demonstra ...