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1. "Informalization" of Food Vending in China From a Tool for Food Security to Employment Promotion

Author:
Taiyang Zhong; Steffanie Scott
Source:
Journal of agriculture, food systems, and community development 2020 v.9 no.4 pp. 135-137
ISSN:
2152-0801
Subject:
Coronavirus infections; agriculture; community development; employment; food security; governance; issues and policy; local government; pandemic; street foods; unemployment; China
Abstract:
... The central government of China required local governments to allow street food vending on May 27, 2020, which is essentially a policy of “informalization” in urban food governance. Before this, some local governments such as Nanjing Municipal Government had already relaxed the implementation of regulations for street food vending. The original purpose of allowing street food vending was to help e ...
DOI:
10.5304/jafscd.2020.094.006

2. "You know nothing, John Doe" – Judgmental overconfidence in lay climate knowledge

Author:
Annina Thaller; Thomas Brudermann
Source:
Journal of environmental psychology 2020 v.69 pp. 101427
ISSN:
0272-4944
Subject:
United Nations Framework Convention on Climate Change; climate; greenhouse gases; issues and policy; ozone; psychology; water vapor
Abstract:
... The 1.5° target outlined in the Paris agreement requires immediate and fundamental climate action; the lack of climate knowledge, prevalent misconceptions and purported knowledge may be major barriers. Therefore, this study was carried out to analyze signs of judgmental overconfidence in lay climate knowledge using a quota sample of 499 Austrians. Results indicate that several misconceptions exist ...
DOI:
10.1016/j.jenvp.2020.101427

3. #Eggs: social and online media-derived perceptions of egg-laying hen housing

Author:
Nicole Widmar; Courtney Bir; Christopher Wolf; John Lai; Yangxuan Liu
Source:
Poultry science 2020 v.99 no.11 pp. 5697-5706
ISSN:
0032-5791
Subject:
agribusiness; business enterprises; cages; consumer attitudes; consumer preferences; decision making; economists; eggs; food industry; foods; laying hens; oviposition; poultry housing; social networks; surveys
Abstract:
... Enormous quantities of data are generated through social and online media in the era of Web 2.0. Understanding consumer perceptions or demand efficiently and cost effectively remains a focus for economists, retailer/consumer sciences, and production industries. Most of the efforts to understand demand for food products rely on reports of past market performance along with survey data. Given the mo ...
DOI:
10.1016/j.psj.2020.07.011

4. 'Eclipse' Thornless Semi-erect Blackberry

Author:
Finn Chad E.; Strik Bernadine C.; Yorgey Brian M.; Peterson Mary E.; Jones Patrick A.; Buller Gil; Serce Sedat; Lee Jungmin; Bassil Nahla V.; Martin Robert R.
Source:
HortScience 2020 v.55 no.5 pp. 749-754
ISSN:
0018-5345
Subject:
Rubus; agronomic traits; blackberries; early development; fruit characteristics; fruit quality; germplasm releases; patents; ripening; thornless varieties
Abstract:
... 'Eclipse' is a thornless, semi-erect, high quality blackberry (Rubus subg. Rubus Watson) that has firm, uniformly shaped, dark fruit suited for the fresh market and that ripen in the early season for this type of blackberry. 'Eclipse' was released by the U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS) breeding program in Corvallis, OR in cooperation with Oregon State Univer ...
Handle:
10113/6973051
DOI:
10.21273/HORTSCI14891-20

5. (2-Carboxyethyl) dimethylsulfonium bromide supplementation in non-fish meal diets for on-growing grass carp (Ctenopharyngodon idella): Beneficial effects on immune function of the immune organs via modulation of NF-κB and TOR signalling pathway

Author:
Pei Wu; Xing-Wei Liu; Lin Feng; Wei-Dan Jiang; Sheng-Yao Kuang; Ling Tang; He-Qun Shi; Xiao-Qiu Zhou; Yang Liu
Source:
Fish & shellfish immunology 2020 v.107 pp. 309-323
ISSN:
1050-4648
Subject:
Ctenopharyngodon idella; animal growth; antimicrobial peptides; aquaculture industry; complement; cytokines; dietary supplements; disease resistance; fish; fish meal; genes; health status; hemorrhage; immune response; immunostimulants; inflammation; kidneys; lysozyme; morbidity; shellfish; spleen; transcription factor NF-kappa B
Abstract:
... The immune function of immune organs is extremely crucial for maintaining organism health status, which ultimately affects fish growth. Our previous study has found that dietary supplementation of (2-carboxyethyl)dimethylsulfonium Bromide (Br-DMPT) in non-fish meal (NFM) diet could promote the growth of grass carp (Ctenopharyngodon idella), whereas the underlying reason or mechanism for this resul ...
DOI:
10.1016/j.fsi.2020.08.032

6. (2-Carboxyethyl)dimethylsulfonium Bromide (Br-DMPT) improves muscle flesh quality and antioxidant status of on-growing grass carp (Ctenopharyngodon idella) fed non-fish meal diets

Author:
Xing-Wei Liu; Lin Feng; Wei-Dan Jiang; Pei Wu; Jun Jiang; De-Min Yang; Ling Tang; Sheng-Yao Kuang; He-Qun Shi; Xiao-Qiu Zhou; Yang Liu
Source:
Aquaculture 2020 v.521 pp. 735065
ISSN:
0044-8486
Subject:
Ctenopharyngodon idella; alanine; antioxidant enzymes; aspartic acid; cathepsins; dietary supplements; docosahexaenoic acid; eicosapentaenoic acid; fish; fish feeding; fish feeds; fish meal; gene expression; glutamic acid; glycine (amino acid); lactic acid; market value; meat quality; muscles; nutritive value; pH; serine; signal transduction; umami; water holding capacity
Abstract:
... High-quality flesh plays crucial roles in affecting the acceptance and market value of the fish products. In previous study, (2-carboxyethyl)dimethylsulfonium Bromide (Br-DMPT) supplementation enhanced the growth and digestive capacity of fish fed non-fish meal diet, whereas its impacts on fish flesh quality remain unknown. Here, we examined whether dietary supplementation with Br-DMPT could impro ...
DOI:
10.1016/j.aquaculture.2020.735065

7. (3 + 2)-Cycloaddition of Donor–Acceptor Cyclopropanes with Selenocyanate: Synthesis of Dihydroselenophenes and Selenophenes

Author:
Anu Jacob; Peter G. Jones; Daniel B. Werz
Source:
Organic letters 2020 v.22 no.21 pp. 8720-8724
ISSN:
1523-7052
Subject:
oxidation; salting; salts; tetramethylammonium compounds
Abstract:
... We present a Lewis-acid-catalyzed (3 + 2)-cycloaddition of donor–acceptor cyclopropanes and selenocyanate (as its tetramethylammonium salt) for the synthesis of dihydroselenophenes. The transformation proceeded with moderate to excellent yields and showed a high functional group tolerance. Further oxidation using DDQ delivered selenophenes. ...
DOI:
10.1021/acs.orglett.0c03329

8. (3,3″)-Linked Biflavanones from Ouratea spectabilis and Their Effects on the Release of Proinflammatory Cytokines in THP-1 Cells

Author:
Marina P. Rocha; Priscilla R. V. Campana; Rodrigo M. Pádua; José D. Souza Filho; Daneel Ferreira; Fernão C. Braga
Source:
Journal of natural products 2020 v.83 no.6 pp. 1891-1898
ISSN:
1520-6025
Subject:
anti-inflammatory agents; bark extracts; cardiovascular diseases; chemokine CCL2; circular dichroism spectroscopy; enantiomers; ethers; inhibitory concentration 50; interleukin-1beta; spectral analysis; tumor necrosis factors; Brazil
Abstract:
... Ouratea spectabilis is an arborous species traditionally used in Brazil as an anti-inflammatory agent. Four new (3,3″)-linked biflavanone O-methyl ethers, named ouratein A (1), B (2), C (3), and D (4), were isolated from the bark extract of the species. Ouratein A (1) is an enantiomer of neochamagesmine A, which has never been described before. The structures were elucidated by extensive spectrosc ...
DOI:
10.1021/acs.jnatprod.0c00074

9. (3R,4S)-isostreptenol III from Penicillium purpurogenum MM

Author:
Mohamed Shaaban
Source:
Natural product research 2020 v.34 no.7 pp. 1032-1036
ISSN:
1478-6427
Subject:
Artemia; Talaromyces purpurogenus; chemical structure; cytotoxicity; fungi; nuclear magnetic resonance spectroscopy
Abstract:
... In continuation of the search for new compounds from the terrestrial fungus Penicillium purpurogenum MM, (3R,4S)-isostreptenol III (1), a further new natural compound is reported. The chemical structure of 1 was confirmed by extensive 1D and 2D NMR and ESI HR mass measurements, and by comparison with literature data. The absolute configuration was determined by ab initio calculations of ECD, ORD, ...
DOI:
10.1080/14786419.2018.1538997

10. (Arylimido)niobium(V)–Alkylidenes, Nb(CHSiMe₃)(NAr)[OC(CF₃)₃](PMe₃)₂, That Enable to Proceed Living Metathesis Polymerization of Internal Alkynes

Author:
Itsuki Izawa; Kotohiro Nomura
Source:
Macromolecules 2020 v.53 no.13 pp. 5266-5279
ISSN:
1520-5835
Subject:
alkynes; catalysts; niobium; polymerization
Abstract:
... Living polymerizations of internal alkynes (2-hexyne, 3-hexyne, 4-methyl-2-pentyne, and 1-phenyl-1-propyne) have been demonstrated at 50 °C by (arylimido)niobium(V)–alkylidene complex catalysts, Nb(CHSiMe₃)(NAr)[OC(CF₃)₃](PMe₃)₂ [Ar = 2,6-Me₂C₆H₃ (4a), 2-MeC₆H₄ (4c), and 2,6-Cl₂C₆H₃ (4d)], in the presence of PMe₃, which plays an essential role to proceed the living polymerization without certain c ...
DOI:
10.1021/acs.macromol.0c00874

11. (Consistently) testing strict exogeneity against the alternative of predeterminedness in linear time-series models

Author:
Alexander Mayer
Source:
Economics letters 2020 v.193 pp. 109335
ISSN:
0165-1765
Subject:
economics; models; sieves; statistics; time series analysis
Abstract:
... A portmanteau and a maximum statistic are proposed to discern strictly exogenous from predetermined regressors. Both test statistics rely on residual cross-correlations, pre-whitened via autoregressive sieves. Limiting normality of the portmanteau statistic and Gumbel-convergence of the maximum statistic are derived. ...
DOI:
10.1016/j.econlet.2020.109335

12. (C₃H₉NI)₄AgBiI₈: a direct-bandgap layered double perovskite based on a short-chain spacer cation for light absorption

Author:
Yunpeng Yao; Bo Kou; Yu Peng; Zhenyue Wu; Lina Li; Sasa Wang; Xinyuan Zhang; Xitao Liu; Junhua Luo
Source:
Chemical communications 2020 v.56 no.21 pp. 3206-3209
ISSN:
1364-548X
Subject:
cations; chemical reactions; iodides; lead; semiconductors
Abstract:
... A new iodide layered double perovskite (C₃H₉NI)₄AgBiI₈ (IPAB) has been developed based on a short-chain spacer cation, which is the first homologous compound in iodide double perovskites that adopt the Ruddlesden–Popper structure type. Importantly, IPAB is a promising environmentally friendly alternative to the recently rapidly progressing lead halide semiconductors owing to its narrow direct-band ...
DOI:
10.1039/c9cc07796k

13. (E)-Piplartine Isolated from Piper pseudoarboreum, a Lead Compound against Leishmaniasis

Author:
Juan C. Ticona; Pablo Bilbao-Ramos; Ninoska Flores; M. Auxiliadora Dea-Ayuela; Francisco Bolás-Fernández; Ignacio A. Jiménez; Isabel L. Bazzocchi
Source:
Foods 2020 v.9 no.9 pp. -
ISSN:
2304-8158
Subject:
Leishmania; Piper; adverse effects; amastigotes; amides; antileishmanials; cutaneous leishmaniasis; foods; fractionation; infection; infectious diseases; lead; leaves; models; parasites; promastigotes; spectroscopy; spleen; therapeutics; toxicity; tropical diseases
Abstract:
... The current therapies of leishmaniasis, the second most widespread neglected tropical disease, have limited effectiveness and toxic side effects. In this regard, natural products play an important role in overcoming the current need for new leishmanicidal agents. The present study reports a bioassay-guided fractionation of the ethanolic extract of leaves of Piper pseudoarboreum against four specie ...
DOI:
10.3390/foods9091250

14. (EX-4)2-Fc, an effective long-acting GLP-1 receptor agonist, reduces obesity-related inflammation by inhibiting leptin expression

Author:
Bailing Zhou; Chunyan Dong; Binyan Zhao; Xiaoqing Su; Yi Luo; Li Xie; Yaomei Tian; Rui Zhang; Li Yang
Source:
Biochemical and biophysical research communications 2020 v.529 no.3 pp. 562-568
ISSN:
0006-291X
Subject:
adipocytes; adipose tissue; agonists; cytokines; drugs; fatty liver; glucagon-like peptide 1; glucose tolerance; immunity; inflammation; leptin; macrophages; mitogen-activated protein kinase; noninsulin-dependent diabetes mellitus; obesity; research; transcription factor NF-kappa B
Abstract:
... Obesity has been recognized as a low-grade, chronic inflammatory disease that leads to an increase in obesity-associated disorders, including type 2 diabetes (T2D), fatty liver diseases and cancer. Glucagon-like peptide-1 (GLP-1) is an effective drug for T2D, and it not only has glucose-regulating effects but also has anti-inflammatory effects in obesity. In our previous study, we designed a novel ...
DOI:
10.1016/j.bbrc.2020.06.054

15. (Fe,N-codoped carbon nanotube)/(Fe-based nanoparticle) nanohybrid derived from Fe-doped g-C3N4: A superior catalyst for oxygen reduction reaction

Author:
Yanpei Zhang; Ruibin Jiang; Zhongke Wang; Yanzhong Xue; Jie Sun; Yingjie Guo
Source:
Journal of colloid and interface science 2020 v.579 pp. 391-400
ISSN:
0021-9797
Subject:
batteries; carbon nanotubes; carbon nitride; carbonization; catalysts; catalytic activity; electrochemistry; fuel cells; iron; moieties; nanohybrids; nanoparticles
Abstract:
... Transition metal- and N-codoped carbon nanotubes (CNTs) have superior catalytic activity because the curling surface enhances the bonding ability of atoms within CNTs to other species. However, it is a great challenge to prepare CNTs with transition metal- and N-doped at high level during the growth of CNTs. Here, (Fe,N-codoped CNT)/(Fe-based nanoparticle) (Fe,N-CNT/FeNP) hybrid nanostructures are ...
DOI:
10.1016/j.jcis.2020.06.057

16. (Fluoro)quinolones and quinolone resistance genes in the aquatic environment: A river catchment perspective

Author:
Erika Castrignanò; Andrew M. Kannan; Kathryn Proctor; Bruce Petrie; Sarah Hodgen; Edward J. Feil; Simon E. Lewis; Luigi Lopardo; Dolores Camacho-Muñoz; Jack Rice; Nick Cartwright; Ruth Barden; Barbara Kasprzyk-Hordern
Source:
Water research 2020 v.182 pp. 116015
ISSN:
0043-1354
Subject:
activated sludge; antibiotic resistance; aquatic environment; ciprofloxacin; epidemiology; flumequine; gene dosage; levofloxacin; lomefloxacin; metabolites; moxifloxacin; nalidixic acid; norfloxacin; people; research; resistance genes; rivers; statistics; stereoselectivity; wastewater; wastewater treatment; water; watersheds; England
Abstract:
... This study provides an insight into the prevalence of (fluoro)quinolones (FQs) and their specific quinolone qnrS resistance gene in the Avon river catchment area receiving treated wastewater from 5 wastewater treatment plants (WWTPs), serving 1.5 million people and accounting for 75% of inhabitants living in the catchment area in the South West of England.. Ofloxacin, ciprofloxacin, nalidixic acid ...
DOI:
10.1016/j.watres.2020.116015

17. (Multi)wavelets increase both accuracy and efficiency of standard Godunov-type hydrodynamic models: Robust 2D approaches

Author:
Georges Kesserwani; Mohammad Kazem Sharifian
Source:
Advances in water resources 2020 v.144 pp. 103693
ISSN:
0309-1708
Subject:
computer software; hydrodynamics; water; wavelet
Abstract:
... Multiwavelets (MW) enable the compression, analysis and assembly of model data on a multiresolution grid within Godunov-type solvers based on second-order discontinuous Galerkin (DG2) and first-order finite volume (FV1) methods. Multiwavelet adaptivity has been studied extensively with one-dimensional (1D) hydrodynamic models (Kesserwani et al., 2019), revealing that MWDG2 can be 20 times faster t ...
DOI:
10.1016/j.advwatres.2020.103693

18. (Non-)Kolbe electrolysis in biomass valorization – a discussion of potential applications

Author:
F. Joschka Holzhäuser; Joel B. Mensah; Regina Palkovits
Source:
Green chemistry 2020 v.22 no.2 pp. 286-301
ISSN:
1463-9270
Subject:
bioeconomics; biomass; biorefining; carbon; carboxylic acids; circular economy; electrochemistry; electrolysis; energy; green chemistry; liquids; raw materials; supply chain
Abstract:
... Driven by the goal of a circular economy, the importance of renewable energies and sustainable sources of raw materials is steadily increasing. The electrochemical conversion of biomass-based compounds into liquid energy sources and basic chemicals enables the direct combination of renewable energies with sustainable carbon sources. A variety of carboxylic acids are efficiently accessible from bio ...
DOI:
10.1039/c9gc03264a

19. (Poly)Phenol Metabolism: A Primer for Practitioners

Author:
Dennis P. Cladis; Connie M. Weaver; Mario G. Ferruzzi
Source:
Nutrition today 2020 v.55 no.5 pp. 234-243
ISSN:
0029-666X
Subject:
absorption; bioactive compounds; diet; digestive tract; excretion; health status; intestinal microorganisms; metabolism; metabolites
Abstract:
... Fruit- and vegetable-derived (poly)phenols have long been lauded for their potential health benefits in humans. Given their ubiquity and diversity in the diet, fully understanding the mechanisms responsible for their health effects is challenging. In light of this, many investigators have sought to understand the absorption, distribution, metabolism, and excretion (ADME) of (poly)phenols, with man ...
DOI:
10.1097/NT.0000000000000433

20. (Z)-3-Hexenol Induces Tea Defense against Ectropis grisescens in the Field

Author:
S. L. Chen; L. P. Zhang; X. M. Cai; L. Bian; Z. X. Luo; Z. Q. Li; L. G. Ge; Z. M. Chen; Z. J. Xin
Source:
Russian journal of plant physiology 2020 v.67 no.3 pp. 572-580
ISSN:
1021-4437
Subject:
Apanteles; Camellia sinensis; Ectropis grisescens; biological control; catechol oxidase; elicitors; insect control; insect larvae; jasmonic acid; leaves; mitogen-activated protein kinase; parasitism; pests; polyphenols; stems; tea; volatile organic compounds
Abstract:
... The tea geometrid Ectropis grisescens is an important pest of tea plant (Camellia sinensis (L.) O. Kuntze). It feeds on the new leaves and tender stems of tea plants, causing serious damage. The green leaf volatile (Z)-3-hexenol (z3HOL) can effectively activate the defense of tea against E. grisescens in the laboratory. In this study, z3HOL was confirmed to activate the tea mitogen-activated prote ...
DOI:
10.1134/S102144372003005X

21. (Z)-3-Hexenyl Butyrate Induces Stomata Closure and Ripening in Vitis vinifera

Author:
Celia Payá; M. Pilar López-Gresa; Diego S. Intrigliolo; Ismael Rodrigo; José María Bellés; Purificación Lisón
Source:
Agronomy 2020 v.10 no.8 pp. -
ISSN:
2073-4395
Subject:
Vitis vinifera; agronomy; anthocyanins; butyrates; color; field experimentation; grapes; ripening; small fruits; stomata; stomatal movement; total soluble solids; vines; viticulture
Abstract:
... Agronomy solutions for modifying pre-harvest grape ripening are needed for a more sustainable viticulture. Field experiments were performed in Vitis vinifera L. vines to study the effect of the previously described stomata-closing compound (Z)-3-hexenyl butyrate (HB). Exogenous treatments at different doses were periodically carried out using a randomized block design. Firstly, we observed that HB ...
DOI:
10.3390/agronomy10081122

22. (Z)-Selective anti-Markovnikov or Markovnikov thiol–yne-click reactions of an internal alkyne by amide hydrogen bond control

Author:
Milan Pramanik; Khokan Choudhuri; Subhayan Chakraborty; Arindam Ghosh; Prasenjit Mal
Source:
Chemical communications 2020 v.56 no.20 pp. 2991-2994
ISSN:
1364-548X
Subject:
alkynes; chemical reactions; hydrogen bonding; regioselectivity
Abstract:
... Herein, we show exclusive control of stereo and regioselective thiol–yne click (TYC) reactions of internal alkynes via amide hydrogen bond control. By exploiting appropriate hydrogen bonding interactions like N–H⋯S, N–H⋯N and C–H⋯O, either (Z)-selective anti-Markovnikov or Markovnikov products could be obtained for an internal alkyne, exclusively. ...
DOI:
10.1039/d0cc00702a

23. (Z)-Trifluoromethyl-Trisubstituted Alkenes or Isoxazolines: Divergent Pathways from the Same Allene

Author:
Chaolun Liu; Glenn P. A. Yap; Casey A. Rowland; Marcus A. Tius
Source:
Organic letters 2020 v.22 no.18 pp. 7208-7212
ISSN:
1523-7052
Subject:
allene; fluorine; protonation
Abstract:
... Because of a charge-dipole interaction involving nonbonding electron pairs on fluorine, protonation of trifluoromethyl allenes leads to tri- or tetrasubstituted alkenes with high (Z)-selectivity. Treatment of the same allenes with catalytic Au(I) initiates a reaction cascade that produces isoxazolines in high yield. ...
DOI:
10.1021/acs.orglett.0c02546

24. (±)-Quassidine K, a pair of cytotoxic bis-β-carboline alkaloid enantiomers from Picrasma quassioides

Author:
Xin-Ming Guo; Fei Li; Fang-Fang Zheng; Na-Na Gong; Yan Li; Wen-Zhou Feng; Lin Tian
Source:
Natural product research 2020 v.34 no.4 pp. 489-493
ISSN:
1478-6427
Subject:
Picrasma quassioides; alkaloids; cytotoxicity; enantiomers; human cell lines; inhibitory concentration 50; spectral analysis
Abstract:
... (±)-Quassidine K (1), a pair of new bis-β-carboline alkaloid enantiomers, were isolated from Picrasma quassioides. Their structures were determined on the basis of detailed spectroscopic data analysis. The absolute configurations of (+)-S-quassidine K (1a) and (−)-R-quassidine K (1b) were determined by comparing with the reported experimental ECD spectra after chiral separation. The cytotoxicity a ...
DOI:
10.1080/14786419.2018.1489388

25. (‐)‐Epicatechin gallate, a metabolite of (‐)‐epigallocatechin gallate in grass carp, exhibits antiviral activity in vitro against grass carp reovirus

Author:
Yitong Zhang; Hao Wang; Meizhen Su; Liqun Lu
Source:
Aquaculture research 2020 v.51 no.4 pp. 1673-1680
ISSN:
1355-557X
Subject:
Ctenopharyngodon idella; Reoviridae; Western blotting; antiviral agents; antiviral properties; aquaculture; catechin; cytopathogenicity; epigallocatechin gallate; green tea; kidneys; metabolites; moieties; pharmacokinetics; stress response
Abstract:
... (‐)‐Epigallocatechin gallate (EGCG), a catechin found in green tea, has been demonstrated to exhibit activity against grass carp reovirus (GCRV). In the current study, we found that EGCG is partially transformed in vivo into (‐)‐epicatechin gallate (ECG), which differs from EGCG only by the absence of a hydroxyl group, and exhibits similar pharmacokinetic behaviour to that of EGCG. ECG is also a m ...
DOI:
10.1111/are.14513

26. (−)-Epicatechin and the comorbidities of obesity

Author:
Eleonora Cremonini; Dario E. Iglesias; Jiye Kang; Giovanni E. Lombardo; Zahra Mostofinejad; Ziwei Wang; Wei Zhu; Patricia I. Oteiza
Source:
Archives of biochemistry and biophysics 2020 v.690 pp. 108505
ISSN:
0003-9861
Subject:
biophysics; cardiovascular diseases; cognition; dysbiosis; endoplasmic reticulum; endotoxins; fatty liver; glucose; human health; humans; inflammation; insulin resistance; lifestyle; mitochondria; noninsulin-dependent diabetes mellitus; obesity; oxidative stress
Abstract:
... Obesity has major adverse consequences on human health contributing to the development of, among others, insulin resistance and type 2 diabetes, cardiovascular disease, non-alcoholic fatty liver disease, altered behavior and cognition, and cancer. Changes in dietary habits and lifestyle could contribute to mitigate the development and/or progression of these pathologies. This review will discuss c ...
DOI:
10.1016/j.abb.2020.108505

27. (−)-Epicatechin reduces muscle waste after complete spinal cord transection in a murine model: role of ubiquitin–proteasome system

Author:
Cristian Gonzalez-Ruiz; Paola Cordero-Anguiano; Axayacatl Morales-Guadarrama; Rodrigo Mondragón-Lozano; Stephanie Sánchez-Torres; Hermelinda Salgado-Ceballos; Francisco Villarreal; Eduardo Meaney; Guillermo Ceballos; Nayelli Nájera
Source:
Molecular biology reports 2020 v.47 no.11 pp. 8975-8985
ISSN:
0301-4851
Subject:
animal injuries; animal models; epicatechin; females; hindlimbs; laminectomy; molecular biology; muscles; protein content; protein degradation; skeletal muscle; spinal cord; ubiquitination; wastes
Abstract:
... The skeletal muscle mass reduces 30–60% after spinal cord injury, this is mostly due to protein degradation through ubiquitin–proteasome system. In this work, we propose that the flavanol (−)-epicatechin, due its widespread biological effects on muscle health, can prevent muscle mass decrease after spinal cord injury. Thirty-six female Long Evans rats were randomized into 5 groups: (1) Spinal cord ...
DOI:
10.1007/s11033-020-05954-x

28. (−)-Hydroxycitric acid regulates energy metabolism by activation of AMPK - PGC1α - NRF1 signal pathway in primary chicken hepatocytes

Author:
Longlong Li; Zhihao Jiang; Yao Yao; Zhongmiao Yang; Haitian Ma
Source:
Life sciences 2020 v.254 pp. 117785
ISSN:
0024-3205
Subject:
6-phosphofructokinase; AMP-activated protein kinase; Garcinia gummi-guta; acetyl-CoA carboxylase; bioactive compounds; carnitine palmitoyltransferase; catabolism; chickens; coenzyme A; diabetes; energy metabolism; fatty-acid synthase; glucose; glycolipids; hepatocytes; lipid metabolism; obesity; peroxisome proliferator-activated receptor gamma; protein content; pyruvate dehydrogenase (lipoamide); signal transduction; succinate dehydrogenase (quinone); triacylglycerols
Abstract:
... As the most important bioactive substance in Garcinia cambogia, (−)-hydroxycitric acid (HCA) is widely used in food additives to regulate obesity and diabetes in animals or humans, while the mechanism is poorly understood. The purpose of this study was to elucidate the regulatory effect and mechanism of (−)-HCA in regulating glucose and lipid metabolism in chicken primary hepatocytes. The results ...
DOI:
10.1016/j.lfs.2020.117785

29. +Chlorination by-products in indoor swimming pools: Development of a pilot pool unit and impact of operating parameters

Author:
Lucie Tsamba; Olivier Correc; Anthony Couzinet
Source:
Environment international 2020 v.137 pp. 105566
ISSN:
0160-4120
Subject:
adverse effects; agitation; byproducts; chlorination; chlorine; disinfection; energy; heat pumps; microbiological quality; swimming pools; water temperature; water treatment
Abstract:
... Chlorine addition in swimming pools ensures the microbiological quality of the water and the bathers’ safety. However, water chlorination is associated with disinfection byproducts (DBP) formation and adverse health effects. The impact of operating parameters and innovative water treatment systems on DBPs levels has been reported in several studies, but sampling campaign in real pools remain diffi ...
DOI:
10.1016/j.envint.2020.105566

30. 1,1′-binaphthyl-2,2′-diamine as a novel MALDI matrix to enhance the in situ imaging of metabolic heterogeneity in lung cancer

Author:
Chenglong Sun; Wei Liu; Yan Mu; Xiao Wang
Source:
Talanta 2020 v.209 pp. 120557
ISSN:
0039-9140
Subject:
amino acids; brain; creatine; fatty acids; histology; image analysis; ions; lung neoplasms; matrix-assisted laser desorption-ionization mass spectrometry; metabolites; mice; nucleosides; nucleotides; peptides; phospholipids; polyamines; potassium; rats; sodium; tissues
Abstract:
... Profile the spatial distributions of endogenous metabolites in heterogeneous tissues is critical to elucidate the complex metabolic mechanisms during pathological progression. Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is a label-free technique for tissue imaging that allows simultaneous localisation and quantification of metabolites in different histological ...
DOI:
10.1016/j.talanta.2019.120557

31. 1,2H hyperfine spectroscopy and DFT modeling unveil the demethylmenasemiquinone binding mode to E. coli nitrate reductase A (NarGHI)

Author:
Maryam Seif Eddine; Frédéric Biaso; Julia Rendon; Eric Pilet; Bruno Guigliarelli; Axel Magalon; Stéphane Grimaldi
Source:
Biochimica et biophysica acta 2020 v.1861 no.8 pp. 148203
ISSN:
0005-2728
Subject:
Escherichia coli; bacteria; deuterium oxide; electron paramagnetic resonance spectroscopy; isoprenoids; isotropy; menaquinones; models; nitrate reductase; oxidation; protons; ubiquinones
Abstract:
... The quinol oxidation site QD in E. coli respiratory nitrate reductase A (EcNarGHI) reacts with the three isoprenoid quinones naturally synthesized by the bacterium, i.e. ubiquinones (UQ), menaquinones (MK) and demethylmenaquinones (DMK). The binding mode of the demethylmenasemiquinone (DMSK) intermediate to the EcNarGHI QD quinol oxidation site is analyzed in detail using ¹,²H hyperfine (hf) spect ...
DOI:
10.1016/j.bbabio.2020.148203

32. 1,3-Dipolar cycloaddition of N-allyl substituted polycyclic derivatives of isoindole-1,3-dione with nitrones and nitrile oxides: An experimental and theoretical investigation

Author:
Mariia M. Efremova; Alexander P. Molchanov; Alexander S. Novikov; Galina L. Starova; Anna A. Muryleva; Alexander V. Slita; Vladimir V. Zarubaev
Source:
Tetrahedron 2020 pp. 131104
ISSN:
0040-4020
Subject:
chemical bonding; chemical structure; cycloaddition reactions; organic compounds; oxides; regioselectivity; stereoselectivity
Abstract:
... Cycloaddition reactions of N-allyl substituted polycyclic derivatives of isoindole-1,3-dione with nitrones proceeds regio- and stereoselectively on the double bond of the N-allyl substituent giving substituted isoxazolidines with good yields. Regioselectivity of the cycloaddition of this dipolarophiles with nitrile oxides depends on the structure of unsaturated hydrocarbon scaffold and the reactio ...
DOI:
10.1016/j.tet.2020.131104

33. 1,3-Dipolar cycloaddition of isatin N,N′-cyclic azomethine imines with α,β-unsaturated aldehydes catalyzed by DBU in water

Author:
Zhan-Yong Wang; Ting Yang; Rongxiang Chen; Xueji Ma; Huan Liu; Kai-Kai Wang
Source:
RSC advances 2020 v.10 no.41 pp. 24288-24292
ISSN:
2046-2069
Subject:
aldehydes; catalytic activity; cycloaddition reactions; diastereoselectivity; heterocyclic compounds; imines; solvents
Abstract:
... A simple and green procedure was established by [3 + 3] cycloaddition reaction of isatin derived cyclic imine 1,3-dipoles with α,β-unsaturated aldehydes, giving the desired spiro heterocyclic oxindoles with aza-quaternary centers in good yields and diastereoselectivities. It should be noted that water can be employed as a suitable solvent for the improvement of diastereoselectivity. ...
DOI:
10.1039/d0ra03806g

34. 1,3-Dipolar cycloaddition reaction on carbohydrate template: Stereoselective synthesis of glycospiroheterocycles

Author:
R. Prasanna; S. Purushothaman; R. Raghunathan
Source:
Tetrahedron 2020 v.76 no.35 pp. 131398
ISSN:
0040-4020
Subject:
X-ray diffraction; condensation reactions; cycloaddition reactions; stereochemistry; stereoselective synthesis; sugars
Abstract:
... A simple and an efficient stereoslective synthesis of glycospiroheterocycles has been accomplished via 1, 3-dipolar cycloaddition (1,3-DC) reaction. The azomethine ylide generated by decarboxylative condensation of N-alkylated α-amino acids with various diketones was trapped by sugar derived dipolarophiles to give glycospiroheterocycles in good yield (72–94%). All the cycloadducts were well charac ...
DOI:
10.1016/j.tet.2020.131398

35. 1,4-Fluoroamination of 1,3-Enynes en Route to Fluorinated Allenes

Author:
Qi Zhang; Munira Taj Muhammad; Mong-Feng Chiou; Yihang Jiao; Hongli Bao; Yajun Li
Source:
Organic letters 2020 v.22 no.13 pp. 5261-5265
ISSN:
1523-7052
Subject:
fluorine; nitrogen; regioselectivity
Abstract:
... The regioselective synthesis of fluorinated allenes via a metal-free 1,4-fluoroamination of 1,3-enynes is presented. This method employs commercially available N-fluorobenzenesulfonimide serving as both the nitrogen source and the fluorine source, affording access to various tetrasubstituted allenes in a straightforward and atom-economic pathway. Preliminary mechanistic studies and theoretical stu ...
DOI:
10.1021/acs.orglett.0c01953

36. 1,4-Hydroboration Reactions of Electron-Poor Aromatic Rings by N-Heterocyclic Carbene Boranes

Author:
Wen Dai; Steven J. Geib; Dennis P. Curran
Source:
Journal of the American Chemical Society 2020 v.142 no.13 pp. 6261-6267
ISSN:
1520-5126
Subject:
aromatic compounds; boranes; carbenes; heterocyclic nitrogen compounds; oxidation; positional isomers; protonation; regioselectivity
Abstract:
... Reactions of N-heterocyclic carbene boranes (NHC-boranes) with electron-poor aromatic rings under photoredox conditions provide dearomatized 3-NHC-boryl-1,5-cycohexadienes, which are formally products of 1,4-hydroboration reactions. When regioisomers are possible, the more crowded (doubly ortho-substituted) product is formed preferably or exclusively. The mechanism may involve oxidation of the NHC ...
DOI:
10.1021/jacs.0c00490

37. 1,4NQ-BC enhances the lung inflammation by mediating the secretion of IL-33 which derived from macrophages

Author:
Qianqian Xiao; Yiming Song; Hongqian Chu; Mengmeng Tang; Jianjun Jiang; Qinghe Meng; Weidong Hao; Xuetao Wei
Source:
Environmental pollution 2020 v.265 pp. 114729
ISSN:
0269-7491
Subject:
carbon; combustion; cytotoxicity; inflammation; innate immunity; interleukin-6; lungs; macrophages; naphthoquinones; particulates; pollution; secretion; vegetation
Abstract:
... Black carbon (BC) is a product of incomplete combustion of fossil fuels and vegetation. The compelling evidence has demonstrated that it has a close relationship with several respiratory and cardiovascular diseases. BC provides the reactive sites and surfaces to absorb various chemicals, such as polycyclic aromatic hydrocarbons (PAH). Naphthoquinone is a typical PAHs which was found in particulate ...
DOI:
10.1016/j.envpol.2020.114729

38. 1,6-Aza-Michael addition of para-quinone methides with N-heterocycles catalyzed by Zn(OTf)2: A regioselective approach to N-diarylmethyl-substituted heterocycles

Author:
Soumitra Guin; Hemonta K. Saha; Ashvani K. Patel; Santosh K. Gudimella; Subhankar Biswas; Sampak Samanta
Source:
Tetrahedron 2020 v.76 no.28-29 pp. 131338
ISSN:
0040-4020
Subject:
carbazoles; moieties; pyrazoles; regioselectivity; saccharin; triazoles
Abstract:
... An efficient Zn(OTf)₂-catalyzed regioselective C–N bond making reaction between a bunch of aryl/heteroaryl-substituted para-quinone methides as ideal 1,6-acceptors and various aromatic/non-aromatic aza-heterocycles bearing N–H moiety namely carbazoles, pyrazoles, indazole, benzotriazole and saccharin is reported. This 1,6-aza-Michael technique delivers predominantly N1-diarylmethyl-substituted het ...
DOI:
10.1016/j.tet.2020.131338

39. 1,7‐Bis(4‐hydroxyphenyl)‐4‐hepten‐3‐one from Betula platyphylla induces apoptosis by suppressing autophagy flux and activating the p38 pathway in lung cancer cells

Author:
Hyun Jin Jung; Kyung‐Sik Song; Youn Kyoung Son; Je Kyung Seong; Sun Yeou Kim; Seung Hyun Oh
Source:
Phytotherapy research 2020 v.34 no.1 pp. 126-138
ISSN:
0951-418X
Subject:
Betula pendula subsp. mandshurica; antineoplastic agents; apoptosis; autophagy; cell cycle checkpoints; cell lines; cell viability; drug therapy; human diseases; lung neoplasms; mitosis; neoplasm cells; protein synthesis; rapamycin
Abstract:
... Betula platyphylla (BP) is frequently administered in the treatment of various human diseases, including cancers. This study was undertaken to investigate the pharmacological function of the active components in BP and the underlying mechanism of its chemotherapeutic effects in human lung cancer cells. We observed that BP extracts and 1,7‐bis(4‐hydroxyphenyl)‐4‐hepten‐3‐one (BE1), one of the compo ...
DOI:
10.1002/ptr.6506

40. 1,8,10-Trisubstituted anthracenyl hydrocarbons: Towards versatile scaffolds for multiple-H-bonded recognition arrays

Author:
Silvia Forensi; Antoine Stopin; Federica de Leo; Johan Wouters; Davide Bonifazi
Source:
Tetrahedron 2020 v.76 no.51 pp. 131299
ISSN:
0040-4020
Subject:
Lewis bases; X-ray diffraction; anthracenes; anthraquinones; boronic acids; cross-coupling reactions; hydrogen bonding; regioselectivity
Abstract:
... In this work, we describe the synthesis of 1,8,10-trisubstituted anthracenyl scaffolds that, bearing boronic acid functionalities, can act as multiple H-bonding donor systems. The trisubstituted anthracenyl derivatives are synthesized following two main synthetic pathways. Whereas in the first approach trisubstituted anthracenyl derivatives are prepared through the regioselective addition of the r ...
DOI:
10.1016/j.tet.2020.131299

41. 1,8-Dioxapyrene-based electrofluorochromic supramolecular hyperbranched polymers

Author:
Shuai Qiu; Zhao Gao; Fei Yan; Hongxing Yuan; Jingxia Wang; Wei Tian
Source:
Chemical communications 2020 v.56 no.3 pp. 383-386
ISSN:
1364-548X
Subject:
chemical reactions; europium; ions; ligands; polymers
Abstract:
... A novel 1,8-dioxapyrene-based electrofluorochromic supramolecular polymer has been successfully constructed, through the coordination of terpyridine ligands with Eu³⁺ ions. The resulting polymer is capable of displaying multicolor tunable capacity and intriguing electrofluorochromic phenomena, and can be used in anti-counterfeiting inks. ...
DOI:
10.1039/c9cc07919j

42. 1-Isoindolinone scaffold-based natural products with a promising diverse bioactivity

Author:
Sunil P. Upadhyay; Pritam Thapa; Ram Sharma; Mukut Sharma
Source:
Fitoterapia 2020 v.146 pp. 104722
ISSN:
0367-326X
Subject:
bioactive properties; carboxylic acids; chemotaxonomy; chronic diseases; medicinal plants; oxidation
Abstract:
... Isoindolin-1-one or 1-isoindolinone framework is referred to phthalimidines or benzo fused γ-lactams of the corresponding γ-amino carboxylic acids and has been of prime interest for scientists for last several decades. 1-Isoindolinone framework is found in a wide range of naturally occurring compounds with diverse biological activities and therapeutic potential for various chronic diseases. Recent ...
DOI:
10.1016/j.fitote.2020.104722

43. 1-Methylcyclopropene on Fruit Quality of Se-Enriched Grape (Vitis vinifera L.) during Shelf Life Period

Author:
Shuaimeng Zhu; Yinli Liang; Lan Mu; Xiaojuan An; Hongfei Yin
Source:
Agronomy 2020 v.10 no.9 pp. -
ISSN:
2073-4395
Subject:
1-methylcyclopropene; Vitis vinifera; ascorbic acid; biofortification; dietary minerals; fertilizer application; fruit quality; fruits; health services; human nutrition; markets; nutrient content; nutrient deficiencies; proanthocyanidins; proteins; raw fruit; ripening; selenium; selenium fertilizers; shelf life; table grapes; titratable acidity; total soluble solids
Abstract:
... Selenium (Se) deficiency in humans could be improved by biofortification of food with selenium, 1-Methylcyclopropene (1-MCP) treatment is beneficial for the non-sulfur storage of fresh fruit. This study investigated fruit quality of table grape (Hutai No.8) with foliar Se fertilizer at maturity, and fruit quality changes of table grape during shelf life stages that caused by postharvest 1-MCP trea ...
DOI:
10.3390/agronomy10091411

44. 10-Fold Quantum Yield Improvement of Ag₂S Nanoparticles by Fine Compositional Tuning

Author:
Alicia Ortega-Rodríguez; Yingli Shen; Irene Zabala Gutierrez; Harrison D. A. Santos; Vivian Torres Vera; Erving Ximendes; Gonzalo Villaverde; José Lifante; Christoph Gerke; Nuria Fernández; Oscar G. Calderón; Sonia Melle; José Marques-Hueso; Diego Mendez-Gonzalez; Marco Laurenti; Callum M. S. Jones; Juan Manuel López-Romero; Rafael Contreras-Cáceres; Daniel Jaque; Jorge Rubio-Retama
Source:
ACS applied materials & interfaces 2020 v.12 no.11 pp. 12500-12509
ISSN:
1944-8252
Subject:
biocompatibility; bioimaging; brain; cardiovascular system; nanoparticles; photoluminescence; semiconductors; silver; silver sulfide
Abstract:
... Ag₂S semiconductor nanoparticles (NPs) are near-infrared luminescent probes with outstanding properties (good biocompatibility, optimum spectral operation range, and easy biofunctionalization) that make them ideal probes for in vivo imaging. Ag₂S NPs have, indeed, made possible amazing challenges including in vivo brain imaging and advanced diagnosis of the cardiovascular system. Despite the conti ...
DOI:
10.1021/acsami.9b22827

45. 100 Years Later, What Is New in Glycerol Bioproduction?

Author:
Marta V. Semkiv; Justyna Ruchala; Kostyantyn V. Dmytruk; Andriy A. Sibirny
Source:
Trends in biotechnology 2020 v.38 no.8 pp. 907-916
ISSN:
0167-7799
Subject:
bisulfites; fermentation; glycerol; liquids; markets; metabolic engineering; osmotolerance; yeasts
Abstract:
... Industrial production of glycerol by yeast, which began during WWI in the so-called Neuberg fermentation, was the first example of metabolic engineering. However, this process, based on bisulfite addition to fermentation liquid, has many drawbacks and was replaced by other methods of glycerol production. Osmotolerant yeasts and other microorganisms that do not require addition of bisulfite to stee ...
DOI:
10.1016/j.tibtech.2020.02.001

46. 100 years of high GEM concentration in the Central Italian Herbarium and Tropical Herbarium Studies Centre (Florence, Italy)

Author:
Jacopo Cabassi; Valentina Rimondi; Zhang Yeqing; Antonella Vacca; Orlando Vaselli; Antonella Buccianti; Pilario Costagliola
Source:
Journal of environmental sciences (China) 2020 v.87 pp. 377-388
ISSN:
1001-0742
Subject:
air; air conditioning; health hazards; herbaria; mercuric chloride; mercury; permissible exposure limit; surveys; ventilation systems; Italy
Abstract:
... Up to 1980s, the most used preservative for herbaria specimens was HgCl2, sublimating at ambient air conditions; ionic Hg then reduces to Hg0 (gaseous elemental mercury, GEM) and diffuses throughout poor ventilated environments. High GEM levels may indeed persist for decades, representing a health hazard. In this study, we present new GEM data from the Central Italian Herbarium and Tropical Herbar ...
DOI:
10.1016/j.jes.2019.07.007

47. 101 Treatment with gonadotrophin-releasing hormone on Day 7 or 21 does not reduce pregnancy loss in dairy heifers receiving invitro-produced embryos

Author:
B. J. Duran; R. V. Sala; P. L. J. Monteiro; C. Gamarra; M. Fosado; E. Walleser; A. Prata; R. Gennari; J. F. Moreno; M. C. Wiltbank; A. Garcia-Guerra
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 176-177
ISSN:
1031-3613
Subject:
Holstein; acetates; body condition; controlled internal drug release devices; corpus luteum; dairy heifers; data collection; embryo transfer; estrus; gonadotropin-releasing hormone; ovulation; pregnancy; progesterone; prostaglandins; regression analysis; ultrasonography
Abstract:
... Previous research has shown that induction of an accessory corpus luteum (CL) by administration of gonadotrophin-releasing hormone (GnRH) on Day 5 increases circulating progesterone and reduces pregnancy loss between Days 33 and 60 in heifers receiving invitro-produced (IVP) embryos. Therefore, the objective of the present study was to determine whether timing of induction of an accessory CL influ ...
DOI:
10.1071/RDv32n2Ab101

48. 103 Effects of administration of human chorionic gonadotrophin at Day 5 post-ovulation on development of the original corpus luteum depend on the locational relationship between the original and accessory corpora lutea

Author:
K. Hazano; S. Haneda; M. Matsui
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 177-178
ISSN:
1031-3613
Subject:
analysis of variance; beef cattle; blood flow; controlled internal drug release devices; corpus luteum; crossbreds; drugs; embryogenesis; estradiol; estrus; gonadotropin-releasing hormone; human chorionic gonadotropin; ovarian follicles; ovulation; prostaglandins; ultrasonography
Abstract:
... In cattle, human chorionic gonadotrophin (hCG) is administered at Day 5 post-ovulation to improve fertility. This treatment can induce ovulation of the first-wave dominant follicle (W1DF), from which an accessory corpus luteum (CL) is generated. In addition, hCG has the effect of promoting CL development. It is possible that the locational relationship between the original and accessary CLs influe ...
DOI:
10.1071/RDv32n2Ab103

49. 106 Comparative growth rates and haematological parameters from calves born by transfer of vitrified invitro-produced embryos and stepbrother calves born by AI

Author:
J. S. Lopes; C. Soriano-Úbeda; L. Sarrias-Gil; E. París-Oller; S. Navarro-Serna; R. Latorre; P. Coy
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 179-180
ISSN:
1031-3613
Subject:
assisted reproductive technologies; blood sampling; body temperature; bulls; calves; cows; culture media; developmental stages; embryo (animal); embryo culture; eosinophils; glucose; heart; heart rate; hematologic tests; jugular vein; progeny; respiratory rate; thorax; withers
Abstract:
... Assisted reproductive technologies (ART) are being extensively used to produce cattle offspring. However, as shown by Siqueira et al. (2017 J. Dairy Sci. 100, 5899-5908; https://doi.org/10.3168/jds.2016-12539), phenotypical and performance differences between cows derived from distinct ART can be found at different stages of development. Thus, in an attempt to mimic the natural environment, reprod ...
DOI:
10.1071/RDv32n2Ab106

50. 108 Use of seminal plasma as ovulation inductor in alpacas (Vicugna pacos) embryo recipient and its effect on pregnancy rate

Author:
W. Huanca; K. Garcia; W. F. Huanca; A. Cordero; J. Malaga
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 181
ISSN:
1031-3613
Subject:
Vicugna pacos; acetates; adults; alpacas; artificial vagina; biotechnology; body condition; breeding programs; corpus luteum; embryo transfer; equine chorionic gonadotropin; females; frozen storage; gonadotropin-releasing hormone; males; nerve growth factor; ovarian follicles; ovulation; pregnancy rate; seminal plasma; spermatozoa; storage temperature; ultrasonography; uterus
Abstract:
... Alpacas, like other camelids, are classified as induced ovulators because an external stimulus is required for the occurrence of ovulation. Recent studies have identified the β-nerve growth factor (β-NGF), a protein present in the seminal plasma (SP), as responsible for inducing ovulation in this species and having potent luteotropic function. We speculate that SP can be used in reproductive biote ...
DOI:
10.1071/RDv32n2Ab108

51. 109 Effect of ruminal infusion with propylene glycol on the invitro embryo production of Holstein (Bos taurus) prepubertal heifers and pregnancy rate of the embryo transfer

Author:
R. Tosta; R. Sala; D. Pereira; D. Kendall; F. Elliff; J. Ziemer; E. Adelsberger; J. Moreno; B. Catussi; P. Baruselli
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 181
ISSN:
1031-3613
Subject:
Holstein; blastocyst; blood; blood sampling; embryo transfer; follicle-stimulating hormone; glucose; heifers; hematologic tests; hexokinase; insulin-like growth factor I; oocytes; pregnancy rate; propylene glycol; puberty; radioimmunoassays
Abstract:
... The aim of this study was to evaluate the effect of ruminal infusion with propylene glycol (PG) on the invitro embryo production (IVEP) of Holstein (Bos taurus) prepubertal heifers (7 to 8 months). For this study, 16 prepubertal heifers were distributed into two groups: Propylene Glycol Group (PGG; n=8) and Control Group (CG; n=7). Additionally, 8 pubertal heifers were used for the positive contro ...
DOI:
10.1071/RDv32n2Ab109

52. 10Be and OSL dating of Pleistocene fluvial terraces along the Hongshuiba River: Constraints on tectonic and climatic drivers for fluvial downcutting across the NE Tibetan Plateau margin, China

Author:
Haibo Yang; Xiaoping Yang; Weiliang Huang; An Li; Zongkai Hu; Xiongnan Huang; Huili Yang
Source:
Geomorphology 2020 v.348 pp. 106884
ISSN:
0169-555X
Subject:
Holocene epoch; Pleistocene epoch; arid zones; climate change; climatic factors; loess; monsoon season; radiative forcing; rivers; sampling; summer; tectonics; terraces; watersheds; China
Abstract:
... Accurate geochronologic constraints of fluvial surfaces along tectonically active mountain fronts can help clarify river responses to tectonic uplift and rapid climate change. In this study, we re-constrained abandonment ages of four fluvial terraces along the Hongshuiba River on the northern margin of the Qilian Shan, NE Tibet using 10Be depth profiles to date fluvial deposits, and OSL dating of ...
DOI:
10.1016/j.geomorph.2019.106884

53. 115 Invivo confocal laser endomicroscopy visualisation of fresh and frozen bull spermatozoa in the genital tract of dairy heifers

Author:
C. Richard; X. Druart; T. Saint-Beuve; V. Gelin; L. Laffont; S. Ruffini; S. Ghazali; M. Saint-Dizier; V. Duranthon; O. Sandra; B. Grimard; V. Mauffre
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 184-185
ISSN:
1031-3613
Subject:
algorithms; blastocyst; bulls; catheters; cervix; chlorides; dairy cows; dairy heifers; dyes; estrus; ewes; freezing; image analysis; in vitro fertilization; phenotype; pregnancy; rhodamines; semen quality; spermatozoa; uterus; vagina
Abstract:
... Fertility in cattle includes the ability of the uterus to provide the appropriate environment for pregnancy success, including the transport of spermatozoa before fertilization. Confocal laser endomicroscopy technology (Cellvizio) has been previously used in small ruminants to visualise labelled spermatozoa invivo in the uterine horns of ewes (Druart et al. 2009 Reprod. 138, 15-53). Nevertheless, ...
DOI:
10.1071/RDv32n2Ab115

54. 118 Semen quality and fertilization ability of myostatin-knockout boars

Author:
M.-F. Xuan; S.-Z. Han; B.-H. Quan; X.-J. Yin; J.-D. Kang
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 186
ISSN:
1031-3613
Subject:
Duroc; acrosome; blastocyst; boars; cell lines; color; ejaculation; genotype; hybrids; in vitro fertilization; mitochondria; odors; oocytes; pH; phenotype; piglets; plasma membrane; pork; progeny; propidium; reproductive performance; semen quality; sexual maturity; sires; somatic cells; sows; staining; t-test
Abstract:
... Myostatin-knockout (MSTN−/−) pigs may provide a source of healthy lean protein for human consumption. However, little is known about the effect of this knockout on semen quality, which is important if these pigs are used for production. The purpose of this study is to evaluate the semen quality and fertility of MSTN−/− boars. We generated MSTN−/− boars from Duroc-Landrace-Yorkshire hybrid pig cell ...
DOI:
10.1071/RDv32n2Ab118

55. 120 Use of fixable dyes to analyze equine sperm membrane integrity and acrosome reaction after A23187 treatment

Author:
I. Ortiz; M. Felix; H. Resende; C. Love; K. Hinrichs
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 187
ISSN:
1031-3613
Subject:
acrosome; acrosome reaction; agglutination; agglutinins; analysis of variance; bovine serum albumin; flow cytometry; fluorescein; horses; in vitro fertilization; lactic acid; propidium; pyruvic acid; semen; sperm capacitation; staining; viability
Abstract:
... Conventional IVF is not successful in the horse, and current work is focused on factors affecting sperm capacitation in this species. Challenges arise in assessing equine sperm incubated in media containing capacitation promotors, as some of these factors cause sperm head-to-head binding (aggregation). Our preliminary microscopic findings showed that sperm aggregates are largely of viable sperm, w ...
DOI:
10.1071/RDv32n2Ab120

56. 121 Effect of pentoxifylline on motility of good- and poor-quality frozen-thawed equine sperm

Author:
M. Felix; I. Ortiz; H. Resende; J. Brom-de-Luna; C. Love; K. Hinrichs
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 187
ISSN:
1031-3613
Subject:
bovine serum albumin; centrifugation; enzyme inhibitors; enzymes; freeze-thaw cycles; freezing; horses; intracytoplasmic sperm injection; lactic acid; pentoxifylline; pyruvic acid; semen; sperm motility; sperm quality; thawing
Abstract:
... Equine semen used for intracytoplasmic sperm injection (ICSI) is typically frozen-thawed and may be of poor quality. To prepare sperm for ICSI, semen is typically centrifuged to remove freezing extender. However, centrifugation can cause damage to sperm, which is especially meaningful if sperm quality is already poor. We evaluated a method for selection of sperm without centrifugation, using a “sw ...
DOI:
10.1071/RDv32n2Ab121

57. 123 Nerve growth factor-induced ovulation in llamas: Evidence of hypothalamic refractoriness to nerve growth factor during the declining phase of the luteinising hormone surge

Author:
R. Carrasco; G. Adams
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 188-189
ISSN:
1031-3613
Subject:
Camelidae; adults; analysis of variance; blood plasma; blood sampling; catheters; copulation; gonadotropin-releasing hormone; hypothalamus; intravenous injection; llamas; luteinizing hormone; mechanism of action; nerve growth factor; nerve tissue; ovarian follicles; ovulation; pharmacokinetics; radioimmunoassays; receptors; seminal plasma; t-test; ultrasonography
Abstract:
... Nerve growth factor (NGF) in semen is responsible for triggering ovulation after copulation in camelids. Interaction of NGF with its cognate receptors results in a preovulatory luteinising hormone (LH) surge that leads to ovulation, but the pharmacokinetics of NGF and the mechanism by which it mediates LH release are unknown. In an effort to elucidate the site and mechanism of action involved, the ...
DOI:
10.1071/RDv32n2Ab123

58. 124 Ovarian follicular development and steroid secretion during oestrous cycle of Lohi sheep

Author:
M. Younis; M. Irfan-ur-Rehman Khan; A. Murtaza; M. Abbas; M. Z. Tahir; K. Javed; I. Mohsin; M. Shahzad
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 189
ISSN:
1031-3613
Subject:
breeding season; corpus luteum; estrous cycle; ewes; follicular development; luteolysis; ovulation; progesterone; prostaglandins; radioimmunoassays; secretion; ultrasonography; Pakistan
Abstract:
... Pakistan has 30.9 million heads of sheep; however, little information is available on their reproductive aspects. The objective of this study was to document ovarian physiology and endocrinology of Lohi ewes during the oestrous cycle. Nine Lohi ewes, synchronized by administering single prostaglandin F2α (PGF2a; Cyclomate, Star Laboratories), were monitored for ovarian follicular dynamics using tr ...
DOI:
10.1071/RDv32n2Ab124

59. 125 Transcriptomic changes in bovine ovarian cortex in response to FSH signaling

Author:
J. Candelaria; B. Rabaglino; A. Denicol
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 189
ISSN:
1031-3613
Subject:
RNA; amino acids; assisted reproductive technologies; biosynthesis; cDNA libraries; carbon dioxide; carbon metabolism; cattle; complementary DNA; computer software; cortex; culture media; energy metabolism; females; follicle-stimulating hormone; follicle-stimulating hormone receptors; follicular development; gene expression regulation; genes; gluconeogenesis; glycolysis; granulosa cells; in vitro culture; mitogen-activated protein kinase; necroptosis; oocytes; phagosomes; sequence analysis; signal transduction; transcriptomics
Abstract:
... Preantral follicles serve as a reservoir of female gametes that could be used in assisted reproductive technologies in humans, livestock, and endangered animals. Invitro culture of ovarian cortex is a widely used method to grow preantral follicles. Follicle-stimulating hormone (FSH) is often added to the culture medium as a folliculogenesis-promoting factor. The roles of FSH in antral follicles is ...
DOI:
10.1071/RDv32n2Ab125

60. 126 Subcortical maternal complex (SCMC) expression during folliculogenesis is affected by oocyte donor age in sheep

Author:
D. Bebbere; A. Abazari-Kia; F. Ariu; L. Bogliolo; S. Ledda
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 190
ISSN:
1031-3613
Subject:
adults; analysis of variance; animal models; arginine deiminase; embryogenesis; female fertility; follicular development; genes; human diseases; humans; linear models; luciferase; maternal effect; messenger RNA; multiprotein complexes; mutation; oocytes; puberty; quantitative polymerase chain reaction; reproductive disorders; sheep; transcription (genetics); zinc finger motif
Abstract:
... Age-associated decline in female fertility is largely attributable to decrease in oocyte quality. However, the molecular mechanisms that shape oocyte developmental competence, and that may be involved in reproductive aging, are yet to be elucidated. The subcortical maternal complex (SCMC) is a multiprotein complex located in the subcortex of oocytes that is essential for early embryogenesis and fe ...
DOI:
10.1071/RDv32n2Ab126

61. 12α-Hydroxylated bile acid induces hepatic steatosis with dysbiosis in rats

Author:
Ja-Young Lee; Hidehisa Shimizu; Masahito Hagio; Satoru Fukiya; Masamichi Watanabe; Yasutake Tanaka; Ga-Hyun Joe; Hitoshi Iwaya; Reika Yoshitsugu; Keidai Kikuchi; Misaki Tsuji; Nanako Baba; Takuma Nose; Koji Tada; Taketo Hanai; Shota Hori; Akari Takeuchi; Yumiko Furukawa; Bungo Shirouchi; Masao Sato; Tadasuke Ooka; Yoshitoshi Ogura; Tetsuya Hayashi; Atsushi Yokota; Satoshi Ishizuka
Source:
Biochimica et biophysica acta 2020 v.1865 no.12 pp. 158811
ISSN:
1388-1981
Subject:
bile; biosynthesis; cecum; cholic acid; deoxycholic acid; diet; dysbiosis; fatty liver; fibrosis; gene expression; inflammation; insulin resistance; intestinal microorganisms; lipogenesis; liver; obesity; oxidative stress; oxysterols; regression analysis; steroid metabolism; taurocholic acid; triacylglycerols
Abstract:
... There is an increasing need to explore the mechanism of the progression of non-alcoholic fatty liver disease. Steroid metabolism is closely linked to hepatic steatosis and steroids are excreted as bile acids (BAs). Here, we demonstrated that feeding WKAH/HkmSlc inbred rats a diet supplemented with cholic acid (CA) at 0.5 g/kg for 13 weeks induced simple steatosis without obesity. Liver triglycerid ...
DOI:
10.1016/j.bbalip.2020.158811

62. 12α-hydroxy-N-demethyl-sauroxine, a lycodane type alkaloid from Phlegmariurus saururus

Author:
Mariana G. Vallejo; Marcos E. Corzo; María Gabriela Ortega; Alicia Mariel Agnese
Source:
Natural product research 2020 v.34 no.9 pp. 1270-1275
ISSN:
1478-6427
Subject:
Lycopodium; acetylcholinesterase; alkaloids; chemical structure; inhibitory concentration 50; spectral analysis; stereochemistry
Abstract:
... 12α-hydroxy-N-demethyl-sauroxine (1), another new Lycopodium alkaloid from the Lycodane group, was isolated from Phlegmariurus saururus (Lam.) B. Øllg. (Lycopodiaceae). Elucidation of the chemical structure and relative stereochemistry were stated by spectroscopic data and chemical correlation. In addition, the inhibitory activity on acetylcholinesterase for 1 was determined as well as for N-methy ...
DOI:
10.1080/14786419.2018.1560287

63. 13 Invitro and invivo embryo production during foal heat in a mare: A case report

Author:
L. F. Campos-Chillon; J. L. Altermatt
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 132
ISSN:
1031-3613
Subject:
Quarter Horse; ambient temperature; blastocyst; carbon dioxide; case studies; cervix; cloprostenol; deslorelin acetate; edema; embryo transfer; estrus; extrusion; foals; freeze-thaw cycles; heat; insemination; intracytoplasmic sperm injection; mares; oocytes; ovarian follicles; ovulation; oxygen; pregnancy; semen; spermatozoa; ultrasonography
Abstract:
... The objective of this case report is to describe invitro and invivo embryo production during foal heat in a mare. A 16-year-old American Quarter Horse mare was presented for breeding and nonsurgical embryo recovery days post-foaling. Upon transrectal ultrasonographic examination, the donor mare was determined to be in oestrous (foal heat) with presence of moderate uterine oedema, a relaxed cervix, ...
DOI:
10.1071/RDv32n2Ab13

64. 13 Years of Storms: An Analysis of the Effects of Storms on Lake Physics on the Atlantic Fringe of Europe

Author:
Mikkel René Andersen; Elvira de Eyto; Mary Dillane; Russell Poole; Eleanor Jennings
Source:
Water 2020 v.12 no.2 pp. -
ISSN:
2073-4441
Subject:
data collection; frequency; lakes; storms; summer; temperature; volume; water; winter; Atlantic Ocean; Ireland; Western European region
Abstract:
... While winter storms are generally common in western Europe, the rarer summer storms may result in more pronounced impacts on lake physics. Using long-term, high frequency datasets of weather and lake thermal structure from the west of Ireland (2005 to 2017), we quantified the effects of storms on the physical conditions in a monomictic, deep lake close to the Atlantic Ocean. We analysed a total of ...
DOI:
10.3390/w12020318

65. 13-Acetoxysarcocrassolide Exhibits Cytotoxic Activity against Oral Cancer Cells through the Interruption of the Keap1/Nrf2/p62/SQSTM1 Pathway: The Need to Move Beyond Classical Concepts

Author:
Yi-Chang Liu; Bo-Rong Peng; Kai-Cheng Hsu; Mohamed El-Shazly; Shou-Ping Shih; Tony Eight Lin; Fu-Wen Kuo; Yi-Cheng Chou; Hung-Yu Lin; Mei-Chin Lu
Source:
Marine drugs 2020 v.18 no.8 pp. -
ISSN:
1660-3397
Subject:
DNA; Western blotting; acetylcysteine; animal models; antineoplastic activity; antineoplastic agents; antioxidant enzymes; apoptosis; cell viability; corals; cytotoxicity; enzyme activity; flow cytometry; fluorescent antibody technique; immunostimulants; mechanism of action; membrane potential; mitochondrial membrane; mouth neoplasms; neoplasm cells; precipitin tests; proteins; reactive oxygen species; small interfering RNA; toxicity testing; transfection; xenotransplantation
Abstract:
... 13-Acetoxysarcocrassolide (13-AC), a marine cytotoxic product isolated from the alcyonacean coral Lobophytum crassum, exhibited potent antitumor and immunostimulant effects as reported in previous studies. However, the 13-AC antitumor mechanism of action against oral cancer cells remains unclear. The activity of 13-AC against Ca9-22 cancer cells was determined using MTT assay, flow cytometric anal ...
DOI:
10.3390/md18080382

66. 131 Genetic variants and haplotype combination in the bovine SH2B2 gene and their associations with molecular breeding for body size traits in Qinchuan cattle (Bos taurus)

Author:
S. H. A. Raza; L. Zan
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 192
ISSN:
1031-3613
Subject:
beef cattle; body length; body weight; breeding programs; carcass quality; cattle breeding; chest; computer software; cytokines; energy balance; exons; genetic variation; genotyping; haplotypes; introns; leptin; linear models; marker-assisted selection; non-specific protein-tyrosine kinase; rump; sequence analysis; single nucleotide polymorphism
Abstract:
... The Src homology 2B 2 (SH2B2) gene regulates energy balance and body weight at least partially by enhancing Janus kinase-2 (JAK2)-mediated cytokine signalling, including leptin or GH signalling. Leptin is an adipose hormone that controls body weight. The objective of the current study was to evaluate the association between body measurement traits and SH2B2 gene polymorphisms. For this purpose, we ...
DOI:
10.1071/RDv32n2Ab131

67. 135 Strategies for transfection of bovine mesenchymal stem cells with pBC1-anti-CD3 vector

Author:
F. B. Duarte; S. N. Báo; M. Brígido; J. M. Araújo; E. d. O. Melo; C. F. Martins
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 194
ISSN:
1031-3613
Subject:
DNA; Southern blotting; T-lymphocytes; adipocytes; adipose tissue; air; autoimmune diseases; bioreactors; carbon dioxide; cattle; culture dishes; fetal bovine serum; fibroblasts; flow cytometry; fluorescence; fluorescence microscopy; funding; genetically modified organisms; human diseases; humidity; mesenchymal stromal cells; monoclonal antibodies; necrosis; neomycin; polymerase chain reaction; transfection
Abstract:
... Cells from different origins behave differently regarding the incorporation of exogenous genetic material and the formation of transgenic cells. In this context, the objective of this study was to verify the potential of transfection of bovine mesenchymal stem cells from Wharton's jelly and adipose tissue, comparing two transfection protocols, using Lipofectamine LTX and Plus or Xfect reagents, wi ...
DOI:
10.1071/RDv32n2Ab135

68. 136 Effects of the homology direct repair enhancer RS-1 on pig embryo culture

Author:
C. Gomes Lucas; B. Bauer; P. Chen; L. Spate; K. Wells; R. Prather
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 194
ISSN:
1031-3613
Subject:
CRISPR-Cas systems; DNA damage; DNA repair; acute exposure; apoptosis; blastocyst; chronic exposure; culture media; dimethyl sulfoxide; disease resistance; embryo culture; embryogenesis; gene editing; genes; human diseases; in vitro fertilization; long term effects; mineral oil; models; nucleases; rabbits; swine; transgenic animals; xenotransplantation; zygote
Abstract:
... Advances in genome editing technologies, such as the CRISPR/Cas9 system, have facilitated gene manipulation and the generation of pigs resistant to disease, models for studying human diseases and for xenotransplantation. However, double-strand breaks generated by the CRISPR/Cas9 system are preferentially repaired by the non-homologous end joining (NHEJ) pathway compared with high-fidelity homology ...
DOI:
10.1071/RDv32n2Ab136

69. 137 Recovery of quail spermatogenesis by donor spermatogonia transplantation

Author:
A. N. Vetokh; E. K. Tomgorova; L. A. Volkova; N. A. Volkova; N. A. Zinovieva
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 195
ISSN:
1031-3613
Subject:
Sertoli cells; blood; body weight; busulfan; cell culture; cell transplantation; drakes; genetic resources; histology; microsatellite repeats; progeny; quails; recombinant DNA; seminiferous tubules; spermatogenesis; spermatogonia; spermatozoa; statistical analysis; stem cells; transgenesis
Abstract:
... Spermatogonia, the stem cell precursors of male germ cells, are used as convenient biological material for the preservation of genetic resources (cryobanks) and the introduction of recombinant DNA (transgenesis). Donor spermatogonia subsequently differentiate into mature germ cells (spermatozoa), which are used to produce offspring. Our laboratory is investigating methods to improve the efficiency ...
DOI:
10.1071/RDv32n2Ab137

70. 13C NMR and XRD studies on the enhancement of cellulose II crystallinity with low concentration NaOH post-treatments

Author:
Satoshi Nomura; Yuki Kugo; Tomoki Erata
Source:
Cellulose 2020 v.27 no.7 pp. 3553-3563
ISSN:
0969-0239
Subject:
X-radiation; X-ray diffraction; alkali treatment; carbon; cellulose; crystal structure; crystallization; crystals; mercerization; neutralization; nuclear magnetic resonance spectroscopy; sodium hydroxide; stable isotopes; sulfuric acid; temperature; wood pulp
Abstract:
... In this study, methods of completing the structural conversion of partly crystalline cellulose II were studied by solid-state ¹³C CP/MAS NMR spectroscopy and X-ray diffraction. The study incorporated post-treatments of samples that had been mercerized or regenerated to yield somewhat crystalline cellulose II. These post-treatments used different temperatures, lower concentrations of NaOH than used ...
DOI:
10.1007/s10570-020-03036-6

71. 14-3-3 proteins contribute to leaf and root development via brassinosteroid insensitive 1 in Arabidopsis thaliana

Author:
Jae Hoon Lee; Geunhwa Kwak; Yong Pyo Lim; Man-Ho Oh
Source:
Genes & genomics 2020 v.42 no.3 pp. 347-354
ISSN:
1976-9571
Subject:
Arabidopsis thaliana; brassinosteroids; cold tolerance; genes; genetic lines; hypocotyls; leaves; mutants; phenotype; phosphorylation; plant growth; protein-serine-threonine kinases; proteins; seedlings; signal transduction; somatotropin; transfer DNA
Abstract:
... BACKGROUND: Brassinosteroids (BR) are essential growth hormone in plants. Various components involved in signal transduction pathway have been identified as targets of 14-3-3 phospho-binding proteins. Previously, we showed that 14-3-3 proteins directly interact with the Brassinosteroid Insensitive 1 (BRI1), the BR receptor kinase, and are also subject to phosphorylation in a BR-dependent manner. O ...
DOI:
10.1007/s13258-019-00909-4

72. 14-Residue peptaibol velutibol A from Trichoderma velutinum: its structural and cytotoxic evaluation

Author:
Varun Pratap Singh; Anup Singh Pathania; Manoj Kushwaha; Samsher Singh; Vandana Sharma; Fayaz A. Malik; Inshad A. Khan; Anil Kumar; Deepika Singh; Ram A. Vishwakarma
Source:
RSC advances 2020 v.10 no.52 pp. 31233-31242
ISSN:
2046-2069
Subject:
DNA damage; Trichoderma; amino acids; apoptosis; assays; cell cycle; cell lines; cytotoxicity; dose response; fungi; habitats; humans; hydrogen bonding; mass; myeloid leukemia; neoplasm cells; peptaibols; stereochemistry; tandem mass spectrometry
Abstract:
... Velutibol A (1), a new 14-residue peptaibol was isolated from the Himalayan cold habitat fungus Trichoderma velutinum. The structural characterization was carried out by 1D and 2D NMR studies, and tandem mass studies, and Marfey's method aided in determining the stereochemistry of the amino acids. The CD analysis revealed folding of the peptide in a 3₁₀-helical conformation. The intramolecular H-b ...
DOI:
10.1039/d0ra05780k

73. 142 Metabolomic analysis of fresh and frozen bovine seminal plasma: A preliminary study

Author:
M. A. Kosior; N. Pagano; A. Staropoli; C. De Canditiis; V. Longobardi; G. Zullo; F. Vinale; B. Gasparrini
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 197-198
ISSN:
1031-3613
Subject:
Holstein; artificial vagina; assisted reproductive technologies; biochemical pathways; bioinformatics; biomarkers; bulls; centrifugation; ceramides; cryopreservation; freeze-thaw cycles; freezing; frozen storage; liquid chromatography; male fertility; mass spectrometry; metabolites; metabolomics; methanol; oxidative stress; screening; seminal plasma; sperm motility; spermatozoa; storage temperature; t-test; Italy
Abstract:
... Sperm cryopreservation is essential for assisted reproduction; however, freezing induces biochemical and physical damage to the sperm membrane structures, negatively affecting sperm fertilising ability (Castro et al. 2006 J. Anim. Sci. Biotechnol. 5, 1-9). Metabolomics is the study of small molecules, commonly known as metabolites, which play essential roles in biological systems. Emerging researc ...
DOI:
10.1071/RDv32n2Ab142

74. 143 Stimulated glycolysis is able to maintain ATP levels and motility of bull spermatozoa submitted to mitochondrial depolarisation

Author:
J. Losano; J. Padín; I. Méndez-López; D. Angrimani; N. Montez; A. García; V. Barnabe; M. Nichi
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 198
ISSN:
1031-3613
Subject:
adenosine triphosphate; albumins; analysis of variance; bulls; cyanides; glucose; glycolysis; lactic acid; luminescence; mitochondria; oxidative phosphorylation; pyruvic acid; spermatozoa
Abstract:
... Studies have reported the importance of mitochondria in sperm metabolism. However, for some species, glycolysis appears to be as essential as oxidative phosphorylation in sperm physiology. On the other hand, these mechanisms have not been fully elucidated for bovine spermatozoa. Therefore, the aim of this study was to evaluate the role of mitochondria and glycolysis in ATP synthesis and sperm kine ...
DOI:
10.1071/RDv32n2Ab143

75. 144 Serum testosterone profile in Marwari stallions and its relationship with testicular parameters, semen characteristics, reaction time, stallion age, bodyweight, and height

Author:
A. K. Chaudhary; G. N. Purohit; J. S. Mehta; S. K. Ravi; T. R. Talluri
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 198
ISSN:
1031-3613
Subject:
artificial vagina; blood sampling; blood serum; body weight; enzyme-linked immunosorbent assay; estrus; mares; semen quality; sperm concentration; sperm motility; stallions; testes; testosterone
Abstract:
... The present study investigated the serum testosterone profile of Marwari stallions before and after exposure to a mare and the relationship of serum testosterone profile with scrotal circumference, semen characteristics, reaction time, stallion age, bodyweight, and height. Marwari stallions (n=9) of three age groups (2-4 years, n=3; 4-6 years, n=3; and 6+ years, n=3) were used in the study. Scrota ...
DOI:
10.1071/RDv32n2Ab144

76. 145 ProAKAP4 concentrations in semen as a predictive tool of bull fertility: A preliminary study

Author:
I. Ruelle; N. Seregeant; D. Bencharif; F. Charreaux; C. Thorin; S. Michaud; M. Dordas-Perpinyà; N. Jouy; S. Audry; C. Maurage; M. Delehedde; L. Briand-Amirat
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 199
ISSN:
1031-3613
Subject:
Holstein; Western blotting; animal models; antibodies; bulls; embryo (animal); enzyme-linked immunosorbent assay; flow cytometry; human reproduction; in vitro fertilization; insemination; knockout mutants; male fertility; mice; microscopy; semen; sperm quality; spermatids; spermatogenesis; straw; testes
Abstract:
... Recently, ProAKAP4 has been described as a pertinent indicator of sperm quality in humans, pigs, and stallions. In knockout mouse models lacking AKAP4 expression, the male mice were infertile. As high proAKAP4 levels were significantly correlated with a lower proportion of abortions in intrauterine insemination settings in human reproduction, proAKAP4 could be considered a pertinent new sperm para ...
DOI:
10.1071/RDv32n2Ab145

77. 148 Bull spermatozoa uptake of extracellular vesicles from bovine seminal plasma

Author:
N. Pagano; M. A. Kosior; B. Gasparrini; V. Longobardi; C. De Canditiis; G. Albero; M. C. Deregibus; G. Bosi; A. Idda; A. Lange Consiglio
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 200
ISSN:
1031-3613
Subject:
Holstein; acrosome; artificial vagina; bulls; cell communication; confocal microscopy; dose response; dyes; fluorescence; fluorescence microscopy; frozen storage; male fertility; messenger RNA; microRNA; non-coding RNA; pellets; proteins; seminal plasma; sperm motility; storage temperature; translation (genetics); ultracentrifugation
Abstract:
... Extracellular vesicles (EV) are important mediators of intercellular communication because they transfer microRNA (miRNA) that are able to repress translation of mRNA. Their presence in seminal plasma suggests a role in sperm fertility. It is known that bull seminal plasma contains fertility-associated proteins that are predictive of high and low fertility (Killian et al. 1993 Biol. Reprod. 49, 12 ...
DOI:
10.1071/RDv32n2Ab148

78. 149 The second isoform of gonadotrophin-releasing hormone and its receptor affect boar semen quality

Author:
C. E. Ross; F. H. Choat; K. N. Plager; A. T. Desaulniers; R. A. Cederberg; G. A. Mills; B. R. White
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 201
ISSN:
1031-3613
Subject:
Leydig cells; antagonists; boars; droplets; genetically modified organisms; gonadotropin-releasing hormone; hormone secretion; kinematics; luteinization; semen quality; seminal plasma; sperm quality; storage temperature; testosterone
Abstract:
... Pigs are the only livestock species encoding a functional protein for both the second isoform of gonadotrophin-releasing hormone (GnRH-II) and its cognate receptor (GnRHR-II). Unlike the classical GnRH system (GnRH-I and GnRHR-I), GnRH-II and GnRHR-II are abundantly produced in porcine testes. Moreover, GnRH-II binding its receptor on Leydig cells stimulates luteinizing hormone-independent testost ...
DOI:
10.1071/RDv32n2Ab149

79. 14C-AMS measurements in modern tree rings to trace local fossil fuel-derived CO2 in the greater Xi'an area, China

Author:
Yaoyao Hou; Weijian Zhou; Peng Cheng; Xiaohu Xiong; Hua Du; Zhenchuan Niu; Xia Yu; Yunchong Fu; Xuefeng Lu
Source:
Science of the total environment 2020 v.715 pp. 136669
ISSN:
0048-9697
Subject:
carbon 14; carbon dioxide; energy; fossils; greenhouse gas emissions; growth rings; socioeconomic development; time series analysis; urban areas; China
Abstract:
... Fossil fuel-derived CO₂ (CO₂ff) time series are critical to understanding urban carbon emissions, and to devise strategies to mitigate emission reduction. Using tree ring ¹⁴C archives, we reconstruct an historical CO₂ff time series from 1991 to 2015 in the greater Xi'an region, China. CO₂ff concentrations from the urban sites reached 22.5 ppm, with an average of 14.0 ppm, while average values from ...
DOI:
10.1016/j.scitotenv.2020.136669

80. 15-Day subchronic developmental toxicity studies of ursolic acid in rats

Author:
Lotte Geerlofs; Zhiyong He; Sa Xiao; Zhi-Cheng Xiao
Source:
Food and chemical toxicology 2020 v.144 pp. 111537
ISSN:
0278-6915
Subject:
adults; body weight; developmental toxicity; fetal development; food consumption; methylcellulose; no observed adverse effect level; polysorbates; progeny; reproductive system; therapeutics; ursolic acid
Abstract:
... Ursolic acid (UA) is a pentacyclic triterpenoid and has the characteristics to serve as a potential therapeutic agent for a range of disorders. However, detailed studies of the toxicity of UA, especially developmental toxicity of UA, are non-existing. The objective of this study was to determine the potential effects of UA on fetal development, adult reproductive system, and major organs. UA was d ...
DOI:
10.1016/j.fct.2020.111537

81. 151 Influence of chorionic gonadotrophin, breeding procedure, and gonadotrophin-releasing hormone on pregnancy, embryo viability, and kidding rate of lactating Alpine goats time inseminated during the early transitional reproductive phase

Author:
M. Calle; L. Dawson; M. Rojas; E. Loetz
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 202
ISSN:
1031-3613
Subject:
anestrus; body condition; body weight; breeding; dairy goats; drugs; embryo (animal); equine chorionic gonadotropin; estrus; gonadotropin-releasing hormone; kidding rate; lactation; milk; ovulation; pregnancy; prostaglandins; solar radiation; viability
Abstract:
... Oestrus and ovulation synchronisation (E/OS) regimens containing equine chorionic gonadotrophin (ECG) are used for follicular stimulation during the early transitional reproductive phase and goat anestrus. However, the effects of ECG when applied at different times are unknown. Thus, the objective was to evaluate the influence of ECG, breeding procedure, and gonadotrophin-releasing hormone (GnRH) ...
DOI:
10.1071/RDv32n2Ab151

82. 154 A method of oviductal semen deposition for use in the goat

Author:
N. Buzzell; S. Blash; K. Miner; M. Schofield; J. Pollock; N. Hawkins; M. Hevy; W. Gavin
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 203-204
ISSN:
1031-3613
Subject:
Saanen; Toggenburg; cattle; cryopreservation; dairy goats; fetus; follicle-stimulating hormone; genetically modified organisms; gonadotropin-releasing hormone; in vitro fertilization; isoflurane; laparotomy; oviducts; pellets; progeny; progesterone; prostaglandins; semen; sperm concentration; sperm quality; straw; superovulation; syringes; tuberculin; uterus; Massachusetts
Abstract:
... The objective of this study was to investigate a method of oviducal semen deposition as a strategy for producing offspring from poor-quality cryopreserved goat sperm. Invitro fertilisation (IVF) and AI are common assisted reproductive technologies used in small ruminants, but they have varied results in the goat. The use of poor-quality cryopreserved-thawed sperm (<50% live/dead ratio at post-thaw ...
DOI:
10.1071/RDv32n2Ab154

83. 158 Optimal time of AI and changes in vaginal mucus characteristics relative to the onset of standing oestrus in Beetal goats

Author:
A. Murtaza; M. Irfan-ur-Rehman Khan; M. Abbas; W. Ahmad; M. Z. Tahir; I. Mohsin
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 205-206
ISSN:
1031-3613
Subject:
Beetal; analysis of variance; breeding season; bucks; chi-square distribution; cloprostenol; color; electrical resistance; estrus; goats; heat; mucus; pregnancy rate; semen; skim milk; spermatozoa
Abstract:
... The aim of this study was to determine the optimum time of AI and its relationship with vaginal mucus characteristics relative to the onset of standing oestrus in Beetal goats. For this purpose, goats (n=257) were synchronised for oestrus by administering two doses of prostaglandin F2α (0.075mg intramuscularly, d+ cloprostenol, Dalmazin) 11 days apart during the breeding season (September-December ...
DOI:
10.1071/RDv32n2Ab158

84. 15N study of the reactivity of atmospheric nitrogen in four mountain forest catchments (Czech Republic, central Europe)

Author:
Frantisek Buzek; Bohuslava Cejkova; Ivana Jackova; Pavel Kram; Filip Oulehle; Oldrich Myska; Jan Curik; Frantisek Veselovsky; Martin Novak
Source:
Applied geochemistry 2020 v.116 pp. 104567
ISSN:
0883-2927
Subject:
ammonium; bogs; denitrification; dissolved inorganic nitrogen; forested watersheds; geochemistry; groundwater; groundwater recharge; growing season; hydrochemistry; hydrodynamics; isotope labeling; lysimeters; mineralization; models; monitoring; nitrates; nitrification; nitrogen; peatlands; pollution; runoff; soil solution; soil water; spring; stable isotopes; storms; streams; winter; Central European region; Czech Republic
Abstract:
... Reactivity of atmospherically deposited nitrate (NO₃⁻) and ammonium (NH₄⁺) was investigated in three mountain forest catchments and one ombrotrophic peat bog located in the northern Czech Republic. The study sites are characterized by moderate to high N pollution rates that are currently decreasing. Monitoring of hydrodynamic data and catchment hydrochemistry (precipitation, soil solutions and run ...
DOI:
10.1016/j.apgeochem.2020.104567

85. 16 Improvement of porcine cloned embryo developmental competence via KDM4A overexpression and H3K9me3 methyltransferase inhibitor treatment

Author:
Y.-T. Zhang; Y. Liu; Z. Liu
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 133
ISSN:
1031-3613
Subject:
blastocyst; computer software; embryogenesis; enzyme inhibitors; epigenetics; fibroblasts; genes; histones; in vitro fertilization; lysine; messenger RNA; methyltransferases; quantitative polymerase chain reaction; somatic cells; swine; transcription (genetics)
Abstract:
... Aberrant epigenetic reprogramming is a major reason for the developmental failure of somatic cell nuclear transfer (SCNT) embryos. Histone H3 lysine 9 trimethylation (H3K9me3), a histone marker for transcriptional repression, is considered a key barrier to the development of cloned embryos. In the present study, we found that H3K9me3 was much higher in SCNT embryos than in IVF embryos at the 4-cel ...
DOI:
10.1071/RDv32n2Ab16

86. 160 Increasing gonadotrophin-releasing hormone dose at initiation of a 5-day CO-Synch protocol increases ovulatory response but not fertility in yearling beef heifers

Author:
E. Rojas Canadas; S. E. Battista; J. Kieffer; S. Wellert; A. Garcia Guerra
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 206-207
ISSN:
1031-3613
Subject:
Angus; acetates; beef cattle; cloprostenol; controlled internal drug release devices; corpus luteum; drugs; gonadotropin-releasing hormone; heifers; ovulation; pregnancy; progesterone; ultrasonography; yearlings; Ohio
Abstract:
... Heifers typically have a reduced ovulation rate following gonadotrophin-releasing hormone (GnRH) application at initiation of a CO-Synch + controlled internal drug release (CIDR) protocol. Thus, the objective of the present study was to determine whether increasing the dose of GnRH at initiation of a 5-day CO-Synch protocol in beef heifers would improve ovulation rate and therefore increase pregna ...
DOI:
10.1071/RDv32n2Ab160

87. 168 Comparison of different Doppler ultrasound settings for pregnancy diagnosis based on corpus luteum perfusion at 21 days after AI in beef cattle

Author:
S. R. Wellert; S. E. Battista; J. Kieffer; R. N. Lurch; A. Garcia-Guerra
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 211
ISSN:
1031-3613
Subject:
beef cattle; blood flow; color; controlled internal drug release devices; corpus luteum; cows; drugs; heifers; insemination; pregnancy; pregnancy diagnosis; regression analysis; reproductive efficiency; ultrasonography; yearlings
Abstract:
... Colour Doppler ultrasonography of the corpus luteum (CL) can be used for early pregnancy diagnosis to improve reproductive efficiency and increase the use of AI in beef cattle. The objective of the present study was to determine the diagnostic performance of different Doppler ultrasonography settings for pregnancy diagnosis based on CL perfusion at 21 days after fixed-time AI in beef heifers and c ...
DOI:
10.1071/RDv32n2Ab168

88. 169 Comparison of pregnancy outcomes in dairy heifers artificially inseminated with sexed semen deposited in the uterine horns versus the uterine body

Author:
S. Kirks; R. Palomares; P. Melendez; M. Ferrer; A. Hoyos; J. Bittar; Z. Turner; M. Ibrahim; J. Gutierrez; D. Lopez; A. Gutierrez; V. Pattarajinda; J. Urdaneta
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 211-212
ISSN:
1031-3613
Subject:
Holstein; animal ovaries; chi-square distribution; controlled internal drug release devices; dairy farming; dairy heifers; dairy herds; drugs; farms; freeze-thaw cycles; gonadotropin-releasing hormone; horses; natural selection; pregnancy outcome; pregnancy rate; progesterone; prostaglandins; reproductive efficiency; semen; spermatozoa; ultrasonography; uterus; Georgia
Abstract:
... The use of sexed semen (SS) for AI of cattle has resulted in lower pregnancy per timed AI (P/TAI) compared with conventional semen. This has been attributed to lower sperm dose and cell damage during the cell sorting-freezing process. Horn AI is a common practice in equines and canines to improve pregnancy rate when using low sperm doses. The objective was to compare P/TAI and pregnancy loss (PL) ...
DOI:
10.1071/RDv32n2Ab169

89. 16S rRNA Sequencing Detected Profftella, Liberibacter, Wolbachia, and Diplorickettsia from Relatives of the Asian Citrus Psyllid

Author:
Atsushi Nakabachi; Igor Malenovský; Ilia Gjonov; Yuu Hirose
Source:
Microbial ecology 2020 v.80 no.2 pp. 410-422
ISSN:
0095-3628
Subject:
Candidatus Liberibacter; Citrus; Diaphorina citri; Oceanospirillales; Psyllidae; Wolbachia; arthropods; bacteria; bacteriome; etiological agents; evolution; fruit diseases; genes; greening disease; horticulture; hosts; insects; microbiome; mutualism; pests; plants (botany); reproduction; ribosomal RNA; sequence analysis; symbionts
Abstract:
... The Asian citrus psyllid Diaphorina citri (Hemiptera: Psylloidea) is a serious pest of citrus species worldwide because it transmits Candidatus Liberibacter spp. (Alphaproteobacteria: Rhizobiales), the causative agents of the incurable citrus disease, huanglongbing or greening disease. Diaphorina citri possesses a specialized organ called a bacteriome, which harbors vertically transmitted intracel ...
DOI:
10.1007/s00248-020-01491-z

90. 16S rRNA Sequencing and Metagenomics Study of Gut Microbiota: Implications of BDB on Type 2 Diabetes Mellitus

Author:
Liang Zhang; Jiao Luo; Xiangqian Li; Shuju Guo; Dayong Shi
Source:
Marine drugs 2020 v.18 no.9 pp. -
ISSN:
1660-3397
Subject:
Bacteroides; Lachnospiraceae; Rhodomela; bacteria; blood glucose; genes; glycemic effect; intestinal microorganisms; metabolic diseases; metabolism; metagenomics; metformin; mice; research; sequence analysis; starch; sucrose
Abstract:
... Gut microbiota has a critical role in metabolic diseases, including type 2 diabetes mellitus (T2DM). 3-bromo-4,5-bis(2,3-dibromo-4,5-dihydroxybenzyl)-1,2-benzenediol (BDB) is a natural bromophenol isolated from marine red alga Rhodomela confervoides. Our latest research showed that BDB could alleviate T2DM in diabetic BKS db mice. To find out whether BDB modulates the composition of the gut microb ...
DOI:
10.3390/md18090469

91. 16S rRNA gene sequencing reveals the relationship between gut microbiota and ovarian development in the swimming crab Portunus trituberculatus

Author:
Hongling Wei; Xing Li; Lei Tang; Hongzhi Yao; Zhiming Ren; Chunlin Wang; Changkao Mu; Ce Shi; Huan Wang
Source:
Chemosphere 2020 v.254 pp. 126891
ISSN:
0045-6535
Subject:
Bifidobacterium; Corynebacterium; Desulfovibrio; Firmicutes; Gemmatimonadetes; Portunus trituberculatus; Rikenella; indicator species; injection; intestinal microorganisms; microbial communities; ovarian development; sampling; sequence analysis
Abstract:
... Gut microbiota executes many beneficial functions. In this study, the relationship between gut microbiota and ovarian development in the swimming crab P. trituberculatus was explored for the first time. A total of 28 phyla and 422 genera were identified across all samples. However, 105 differential operational taxonomic units, and four differential phyla (Gemmatimonadetes, Actinobacteria, Firmicut ...
DOI:
10.1016/j.chemosphere.2020.126891

92. 17-DMAG disrupted the autophagy flux leading to the apoptosis of acute lymphoblastic leukemia cells by inducing heat shock cognate protein 70

Author:
Gang Xu; Xiujuan Ma; Fang Chen; Di Wu; Jianing Miao; Yang Fan
Source:
Life sciences 2020 v.249 pp. 117532
ISSN:
0024-3205
Subject:
RNA interference; apoptosis; autophagosomes; autophagy; cathepsin D; cell lines; cell viability; children; children at risk; enzyme inhibition; flow cytometry; fluorescent antibody technique; gene expression; gene targeting; heat stress; heat-shock protein 90; image analysis; immunoblotting; lymphocytic leukemia; lysosomes; messenger RNA; neoplasm cells; patients; pharmacology; protein synthesis; staining; water solubility
Abstract:
... B-lineage acute lymphoblastic leukemia (B-ALL) is most common in children. We had reported heat shock protein 90 (Hsp90) over-expressed in high risk B-ALL children. 17-DMAG is a water soluble Hsp90 inhibitor, which was proved to be effective for advanced solid tumors and hematological malignancy. However, there is little research on its application in newly diagnosed B-ALL. And the detailed mechan ...
DOI:
10.1016/j.lfs.2020.117532

93. 17-Oxo-docosahexaenoic acid induces Nrf2-mediated expression of heme oxygenase-1 in mouse skin in vivo and in cultured murine epidermal cells

Author:
Muhammad Usman Jamil; Jimin Kim; Hye-Won Yum; Seong Hoon Kim; Su-Jung Kim; Do-Hee Kim; Nam-Chul Cho; Hye-Kyung Na; Young-Joon Surh
Source:
Archives of biochemistry and biophysics 2020 v.679 pp. 108156
ISSN:
0003-9861
Subject:
NAD(P)H dehydrogenase (quinone); antioxidant activity; cysteine; docosahexaenoic acid; embryo (animal); fibroblasts; heme oxygenase (biliverdin-producing); mice; mutation; omega-3 fatty acids; stress response; topical application; transcription (genetics); transcription factors; ubiquitination
Abstract:
... Recently, growing attention has been given to new classes of bioactive lipid mediators derived from ω-3 polyunsaturated fatty acids, such as docosahexaenoic acid (DHA), especially in the context of their role as endogenous signal modulators. One such molecule is 17-oxo-DHA, generated from DHA by the action of COX2 and a dehydrogenase. The redox-sensitive transcription factor, Nrf2 plays a key role ...
DOI:
10.1016/j.abb.2019.108156

94. 17-α-Ethinylestradiol modulates endocrine and behavioral responses to stress in zebrafish

Author:
Lurian Fenske; Ani Carla Concato; Ana Paula Vanin; Wagner Antonio Tamagno; Jéssica Reis de Oliveira Sofiatti; Helen Treichel; João Gabriel Santos da Rosa; Leonardo José Gil Barcellos; Rosilene R. Kaizer
Source:
Environmental science and pollution research international 2020 v.27 no.23 pp. 29341-29351
ISSN:
0944-1344
Subject:
Danio rerio; aggression; aquatic environment; contraceptives; endocrine-disrupting chemicals; pollution; predator-prey relationships; reproduction; research; social cohesion; steroid hormones; synthetic estrogens; tranquilizers
Abstract:
... The synthetic estrogen, 17-α-ethinylestradiol (EE2), present in contraceptive pills, is an endocrine-disrupting chemical (EDC) that can be found in the aquatic environment. We examined the impacts of EE2 on zebrafish behavioral and physiological responses through the novel tank test (NTT), which measures anxiety-like behavior; the mirror-induced aggression (MIA) test, which measures aggressiveness ...
DOI:
10.1007/s11356-020-09318-7

95. 172 Effect of period of insertion of a progesterone-releasing device and pro-oestrus length on follicular and luteal characteristics and pregnancy rates to fixed-time AI in Bos indicus heifers

Author:
G. A. Bo; A. Cedeño; R. Maingón; J. P. Cedeño; H. Gamboa; J. Avellan; J. Bravo; C. Rivera; I. Macias
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 213
ISSN:
1031-3613
Subject:
beef cattle; body condition; cloprostenol; corpus luteum; equine chorionic gonadotropin; estradiol; estrus; gonadotropin-releasing hormone; heifers; intravaginal devices; ovulation; pregnancy rate; proestrus; progesterone; ultrasonography; zebu
Abstract:
... An experiment was designed to evaluate the effect of the length of insertion of a progesterone (P4)-releasing device and the length of pro-oestrus on follicular and luteal characteristics and pregnancy rates to AI (P/AI) in Bos indicus heifers treated with oestradiol/P4-based treatments. Bos indicus beef heifers (n=374), 22-26 months of age, with a corpus luteum (CL) or at least one follicle ≥8mm ...
DOI:
10.1071/RDv32n2Ab172

96. 178 Evaluation of polar body extrusion following exposure of cattle oocytes to different concentrations of ethylene glycol cryoprotectant

Author:
M. L. Mphaphathi; M. Nkadimeng; S. M. Sithole; M. D. Sebopela; F. V. Ramukhithi; M. M. Tshabalala; A. Maqhashu; H. O'Neill; T. L. Nedambale
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 217
ISSN:
1031-3613
Subject:
carbon dioxide; cell death; cows; cryoprotectants; cytoplasm; ethylene glycol; extrusion; granulosa cells; image analysis; oocytes; osmotic stress; saline water; slaughter; slaughterhouses; t-test; toxicity; toxicity testing
Abstract:
... Cryoprotectant (CPA) toxicity has been recognised as a critical barrier to further advancement of cattle oocytes. Furthermore, CPA at high concentration might be toxic to oocytes, leading to osmotic shock and cell death (Arav et al. 1993). Selection of quality oocytes among a heterogeneous pool is mostly done subjectively. The use of Brilliant Cresyl Blue (BCB) stain for oocyte selection before et ...
DOI:
10.1071/RDv32n2Ab178

97. 17β-Estradiol affects the innate immune response in common carp

Author:
Magdalena Maciuszek; Lukasz Pijanowski; Agnieszka Pekala-Safinska; B. M. Lidy Verburg-van Kemenade; Magdalena Chadzinska
Source:
Fish physiology and biochemistry 2020 v.46 no.5 pp. 1775-1794
ISSN:
0920-1742
Subject:
Aeromonas; Cyprinus carpio; arginase; aromatase; carp; chemokines; estradiol; estrogen receptors; fish diseases; gene expression; gene expression regulation; genes; immune response; immunomodulation; inflammation; innate immunity; interleukin-10; interleukin-12; kidneys; lipopolysaccharides; macrophages; pathogens
Abstract:
... Inflammation is the evolutionary conserved immune response to harmful stimuli such as pathogens or damaged cells. This multistep process acts by removing injurious stimuli and initiating the healing process. Therefore, it must be tightly regulated by cytokines, chemokines, and enzymes, as well as neuroendocrine mediators. In the present work, we studied the immunoregulatory properties of 17β-estra ...
DOI:
10.1007/s10695-020-00827-3

98. 17β-Estradiol improves insulin signalling and insulin resistance in the aged female hearts: Role of inflammatory and anti-inflammatory cytokines

Author:
Mansour Esmailidehaj; Fatemeh Kuchakzade; Mohammad Ebrahim Rezvani; Zeinab Farhadi; Hassan Esmaeili; Hossein Azizian
Source:
Life sciences 2020 v.253 pp. 117673
ISSN:
0024-3205
Subject:
blood glucose; cardiac output; cardiovascular diseases; energy balance; estradiol; fasting; females; glucose transporters; heart; insulin; insulin resistance; interleukin-10; interleukin-1beta; metabolism; ovariectomy; plasma membrane; postmenopause; premenopause; rats; risk; signal transduction; tissues; tumor necrosis factor-alpha; women
Abstract:
... Aging effects in energy balance in all tissues and organs, including the cardiovascular. The risk of cardiovascular disease is drastically higher in postmenopausal women than in premenopausal women. Estrogen plays an important role in the cardiac function and body's metabolism. The aim of this study was to determine whether 17β-estradiol (E2) has beneficial effects on insulin resistance and some k ...
DOI:
10.1016/j.lfs.2020.117673

99. 17β-Estradiol removal routes by moving bed biofilm reactors (MBBRs) under various C/N ratios

Author:
Changwei Li; Zhefeng Gu; Songming Zhu; Dezhao Liu
Source:
Science of the total environment 2020 v.741 pp. 140381
ISSN:
0048-9697
Subject:
adsorption; ammonia; biofilm; environment; nitrification
Abstract:
... This study evaluated the contribution of biotic and abiotic routes to the 17β-estradiol (E2) removal in moving bed biofilm reactors (MBBRs), and uncovered the interrelation between the E2 removal routes and biofilm characteristics, which was not researched in previous literature. Three MBBRs with different C/N ratios (0 for C/N₀; 2 for C/N₂; and 5 for C/N₅) were operated in continuous mode. A 65-d ...
DOI:
10.1016/j.scitotenv.2020.140381

100. 17β-estradiol attenuates rat articular chondrocyte injury by targeting ASIC1a-mediated apoptosis

Author:
Su-Jing Song; Jing-Jing Tao; Shu-Fang Li; Xue-Wen Qian; Ruo-Wen Niu; Cong Wang; Yi-Hao Zhang; Yong Chen; Ke Wang; Fei Zhu; Chuan-Jun Zhu; Gang-Gang Ma; Yang-Yang; Xiao-Qing Peng; Ren-Peng Zhou; Fei-Hu Chen
Source:
Molecular and cellular endocrinology 2020 v.505 pp. 110742
ISSN:
0303-7207
Subject:
apoptosis; cartilage; chondrocytes; estradiol; etiology; inflammation; ion channels; metabolism; mitochondria; models; ovariectomy; pathogenesis; protective effect; protein content; protein degradation; rheumatoid arthritis
Abstract:
... Epidemiological evidence suggests that the etiology and pathogenesis of rheumatoid arthritis (RA) are closely associated with estrogen metabolism and deficiency. Estrogen protects against articular damage. Estradiol replacement therapy ameliorates local inflammation and knee joint swelling in ovariectomized models of RA. The mechanistic basis for the protective role of 17β-estradiol (17β-E2) is po ...
DOI:
10.1016/j.mce.2020.110742