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You searched for: Publication year rev 7980-2020 Remove constraint Publication year rev: 7980-2020 Text availability Full Text Remove constraint Text availability: Full Text
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1. #GoingtotheFair: a social media listening analysis of agricultural fairs1

Author:
Julie A. Mahoney; Nicole J. O. Widmar; Courtney L. Bir
Source:
Translational Animal Science 2020 v.4 no.3 pp. 1-13
ISSN:
2573-2102
Subject:
agricultural exhibitions; beef cattle; biosecurity; data analysis; income; poultry; public health; risk; swine; swine influenza; zoonoses
Abstract:
... Agricultural fairs provide one of the last frontiers, and largest stages, for showcasing livestock agriculture to the public. However, public funding, attendance revenue, animal biosecurity, and public health concerns are all aspects worthy of conversation and increased research attention given the interaction between livestock animals and the general public in fair and festival settings. A promin ...
DOI:
10.1093/tas/txaa139
CHORUS:
10.1093/tas/txaa139

2. (-)-Epicatechin and NADPH oxidase inhibitors prevent bile acid-induced Caco-2 monolayer permeabilization through ERK1/2 modulation

Author:
Ziwei Wang; M. Corina Litterio; Michael Muller; David Vauzour; Patricia I. Oteiza
Source:
Redox Biology 2020 v.28 no. pp. -
ISSN:
2213-2317
Subject:
NAD(P)H oxidase (H2O2-forming); bile; deoxycholic acid; digestive tract; epithelial cells; human cell lines; inflammation; mitochondria; models; myosin light chains; occludins; oxidants; permeability; phosphorylation; tight junctions
Abstract:
... Secondary bile acids promote gastrointestinal (GI) tract permeabilization both in vivo and in vitro. Consumption of high fat diets increases bile acid levels in the GI tract which can contribute to intestinal permeabilization and consequent local and systemic inflammation. This work investigated the mechanisms involved in bile acid (deoxycholic acid (DCA))-induced intestinal epithelial cell monola ...
Handle:
10113/7331452
DOI:
10.1016/j.redox.2019.101360
CHORUS:
10.1016/j.redox.2019.101360

3. (−)-Epicatechin mitigates high fat diet-induced neuroinflammation and altered behavior in mice

Author:
Jiye Kang; Ziwei Wang; Patricia I. Oteiza
Source:
Food & function 2020 v.11 no.6 pp. 5065-5076
ISSN:
2042-650X
Subject:
anti-inflammatory activity; cognition; dietary supplements; endotoxemia; high fat diet; inflammation; lead; males; memory; memory disorders; mice; obesity; oxidative stress; water
Abstract:
... Obesity is characterized by a condition of low-level chronic inflammation that can lead to altered cognition and behavior. The flavanol (−)-epicatechin (EC) has been shown to have anti-inflammatory actions in mouse models of diet-induced obesity. This study investigated the capacity of dietary EC to mitigate hippocampal inflammation and impaired memory in high fat diet (HFD)-fed mice. Healthy 6 we ...
DOI:
10.1039/d0fo00486c
CHORUS:
10.1039/d0fo00486c

4. 1,4-D-Glucan block copolymers: synthesis and comprehensive structural characterization

Author:
Payam Hashemi; Petra Mischnick
Source:
Analytical and bioanalytical chemistry 2020 v.412 no.7 pp. 1597-1610
ISSN:
1618-2642
Subject:
Fourier transform infrared spectroscopy; composite polymers; dissociation; electrospray ionization mass spectrometry; glucans; glycosylation; liquid chromatography; methylation; models; nuclear magnetic resonance spectroscopy; tandem mass spectrometry
Abstract:
... Multi-block glucans comprising permethylated and partially methylated blocks are compounds of interest. In order to monitor their formation by transglycosylation of corresponding starting glucans, a method has been developed and applied to model compounds. This method allows determining the average length of the blocks and the progress of incorporation of methyl blocks in partially methylated sequ ...
DOI:
10.1007/s00216-020-02388-z
PubMed:
31960080
PubMed Central:
PMC7026270

5. 101 Dothideomycetes genomes: A test case for predicting lifestyles and emergence of pathogens

Author:
S. Haridas; R. Albert; M. Binder; J. Bloem; K. LaButti; A. Salamov; B. Andreopoulos; S.E. Baker; K. Barry; G. Bills; B.H. Bluhm; C. Cannon; R. Castanera; D.E. Culley; C. Daum; D. Ezra; J.B. González; B. Henrissat; A. Kuo; C. Liang; A. Lipzen; F. Lutzoni; J. Magnuson; S.J. Mondo; M. Nolan; R.A. Ohm; J. Pangilinan; H.-J. Park; L. Ramírez; M. Alfaro; H. Sun; A. Tritt; Y. Yoshinaga; L.-H. Zwiers; B.G. Turgeon; S.B. Goodwin; J.W. Spatafora; P.W. Crous; I.V. Grigoriev
Source:
Studies in mycology 2020 v.96 pp. 141-153
ISSN:
0166-0616
Subject:
Dothideomycetes; artificial intelligence; biomass; climate change; feedstocks; food crops; fuel production; fungi; genes; host specificity; livestock; niches; pathogenicity; phylogeny; plant pathogens; prediction; taxonomic revisions
Abstract:
... Dothideomycetes is the largest class of kingdom Fungi and comprises an incredible diversity of lifestyles, many of which have evolved multiple times. Plant pathogens represent a major ecological niche of the class Dothideomycetes and they are known to infect most major food crops and feedstocks for biomass and biofuel production. Studying the ecology and evolution of Dothideomycetes has significan ...
Handle:
10113/6829815
DOI:
10.1016/j.simyco.2020.01.003
PubMed:
32206138
PubMed Central:
PMC7082219

6. 13C analysis of fatty acid fragments by gas chromatography mass spectrometry for metabolic flux analysis

Author:
Weichao Wu; Paul Dijkstra; Michaela A. Dippold
Source:
Geochimica et Cosmochimica Acta 2020 v.284 no. pp. 92-106
ISSN:
0016-7037
Subject:
Bacillus licheniformis; Pseudomonas fluorescens; fatty acids; gas chromatography-mass spectrometry; glucose; hydrogen; metabolic flux analysis; pentose phosphate cycle; propionic acid
Abstract:
... When multiple metabolic pathways lead to the same product, compound-specific isotope analysis may not provide enough information to quantify the activities of the contributing pathways. Instead, identification of where in the molecule the 13C is incorporated is required. Here we show how knowledge of position-specific 13C incorporation in fatty acids (FA) and FA fragments can be used to quantitati ...
DOI:
10.1016/j.gca.2020.05.032
CHORUS:
10.1016/j.gca.2020.05.032

7. 16S rRNA Gene-Based Analysis Reveals the Effects of Gestational Diabetes on the Gut Microbiota of Mice During Pregnancy

Author:
Ziting Yao; Yu Long; Juan Ye; Pu Li; Yonghua Jiang; Yanming Chen
Source:
Indian journal of microbiology 2020 v.60 no.2 pp. 239-245
ISSN:
0046-8991
Subject:
animal disease models; bacterial communities; community structure; embryo (animal); gestational diabetes; hyperglycemia; intestinal microorganisms; intestines; mice; pregnancy; pregnant women; probiotics; ribosomal RNA; streptozotocin; trophic relationships
Abstract:
... Hyperglycemia is one of the metabolic characteristics of gestational diabetes mellitus (GDM). Considering that GDM is able to cause changes in the gut bacterial community and function in the mother’s intestine compared with healthy pregnant women, we aimed to clarify the correlation between hyperglycemia and gut microbiota in a GDM mouse model. Mice were divided into four groups: CE0, GDME0, CE18, ...
DOI:
10.1007/s12088-020-00862-x
PubMed:
32372772
PubMed Central:
PMC7194076

8. 17β-estradiol promotes acute refeeding in hungry mice via membrane-initiated ERα signaling

Author:
Kaifan Yu; Yanlin He; Ilirjana Hyseni; Zhou Pei; Yongjie Yang; Pingwen Xu; Xing Cai; Hesong Liu; Na Qu; Hailan Liu; Yang He; Meng Yu; Chen Liang; Tingting Yang; Julia Wang; Pierre Gourdy; Jean-Francois Arnal; Francoise Lenfant; Yong Xu; Chunmei Wang
Source:
Molecular Metabolism 2020 v.42 no. pp. -
ISSN:
2212-8778
Subject:
electrophysiology; energy balance; estrogen receptors; fasting; females; homeostasis; hypoglycemia; hypothalamus; metabolism; mutants; mutation; obesity; ovariectomy; starvation; transcription (genetics)
Abstract:
... Objective Estrogen protects animals from obesity through estrogen receptor α (ERα), partially by inhibiting overeating in animals fed ad libitum. However, the effects of estrogen on feeding behavior in hungry animals remain unclear. In this study, we examined the roles of 17β-estradiol (E2) and ERα in the regulation of feeding in hungry female animals and explored the underlying mechanisms. Method ...
Handle:
10113/7442874
DOI:
10.1016/j.molmet.2020.101053
CHORUS:
10.1016/j.molmet.2020.101053

9. 2-Nitro-1-propanol improved nutrient digestibility and oocyst shedding but not growth performance of Eimeria-challenged broilers

Author:
Po-Yun Teng; Sudhir Yadav; Tatiane Souza dos Santos; Alberta Lorraine Fuller; Woo Kyun Kim
Source:
Poultry science 2020 v.99 no.9 pp. 4314-4322
ISSN:
0032-5791
Subject:
Eimeria acervulina; Eimeria maxima; Eimeria tenella; broiler chickens; cages; dextran; diet; digestibility; digestible energy; dose response; fluorescein; growth performance; ileum; isothiocyanates; males; nitrogen; oocysts; permeability; villi
Abstract:
... A 2 × 3 factorial arrangement study was conducted to evaluate 3 dosages of 2-nitro-1-propanol (NP; 0, 150, and 200 ppm) on intestinal health of birds with or without Eimeria challenge. A total of 432 thirteen-day-old male broiler chickens were randomly allocated to 6 treatments with 8 replicate cages of 9 birds per cage. All birds were fed with treatment diets from day 13 to 21. Birds in the chall ...
DOI:
10.1016/j.psj.2020.05.038
CHORUS:
10.1016/j.psj.2020.05.038

10. 2-Phenylethanol and 2-phenylethylacetate production by nonconventional yeasts using tequila vinasses as a substrate

Author:
José de Jesús Rodríguez-Romero; César Arturo Aceves-Lara; Cristina Ferreira Silva; Anne Gschaedler; Lorena Amaya-Delgado; Javier Arrizon
Source:
Biotechnology reports 2020 v.25 pp. e00420
ISSN:
2215-017X
Subject:
Candida glabrata; Candida parapsilosis; Candida utilis; Wickerhamomyces anomalus; biomass production; fermentation; guidelines; industrial applications; odor compounds; oxygen; phenylethyl alcohol; shikimate pathway; value-added products; vinasse; wastewater; yeasts
Abstract:
... Vinasses from the tequila industry are wastewaters with highly elevated organic loads. Therefore, to obtain value-added products by yeast fermentations, such as 2-phenylethanol (2-PE) and 2-phenylethylacetate (2-PEA), could be interesting for industrial applications from tequila vinasses. In this study, four yeasts species (Wickerhamomyces anomalus, Candida glabrata, Candida utilis, and Candida pa ...
DOI:
10.1016/j.btre.2020.e00420
PubMed:
32025510
PubMed Central:
PMC6997672

11. 20-hydroxyeicosatetraenoic acid alters endothelial cell barrier integrity independent of oxidative stress and cell death

Author:
Vengai Mavangira; Jennifer Brown; Jeffery C. Gandy; Lorraine M. Sordillo
Source:
Prostaglandins and Other Lipid Mediators 2020 v.149 no. pp. -
ISSN:
1098-8823
Subject:
antioxidants; aorta; apoptosis; bovine mastitis; cattle; coliform bacteria; endothelial cells; glutathione; inflammation; metabolites; models; oxidative stress; prostaglandins; selenium; therapeutics; vitamin E
Abstract:
... Unregulated inflammation during bovine mastitis is characterized by severe mammary tissue damage with systemic involvement. Vascular dysfunction underlies tissue pathology because of concurrent oxidative stress mediated by several inflammatory mediators. We recently demonstrated increased production of 20-hydroxyeicosatetraenoic acid (20-HETE), a cytochrome P450-derived (CYP) oxylipid that correla ...
Handle:
10113/7448363
DOI:
10.1016/j.prostaglandins.2020.106425
CHORUS:
10.1016/j.prostaglandins.2020.106425

12. 2019_nCoV/SARS‐CoV‐2: rapid classification of betacoronaviruses and identification of Traditional Chinese Medicine as potential origin of zoonotic coronaviruses

Author:
T.M. Wassenaar; Y. Zou
Source:
Letters in applied microbiology 2020 v.70 no.5 pp. 342-348
ISSN:
0266-8254
Subject:
Betacoronavirus; Chiroptera; Oriental traditional medicine; disease outbreaks; genetic relationships; genetic similarity; genome; risk; sequence analysis; viruses; zoonoses; China
Abstract:
... The current outbreak of a novel severe acute respiratory syndrome‐like coronavirus, 2019_nCoV (now named SARS‐CoV‐2), illustrated difficulties in identifying a novel coronavirus and its natural host, as the coding sequences of various Betacoronavirus species can be highly diverse. By means of whole‐genome sequence comparisons, we demonstrate that the noncoding flanks of the viral genome can be use ...
DOI:
10.1111/lam.13285
PubMed:
32060933
PubMed Central:
PMC7165814

13. 2D-Infrared Spectroscopy of Proteins in Water: Using the Solvent Thermal Response as an Internal Standard

Author:
Samantha Hume; Gregory M. Greetham; Paul M. Donaldson; Michael Towrie; Anthony W. Parker; Matthew J. Baker; Neil T. Hunt
Source:
Analytical chemistry 2020 v.92 no.4 pp. 3463-3469
ISSN:
1520-6882
Subject:
automation; calibration; deuterium oxide; high-throughput screening methods; proteins; screening; solvents; spectral analysis; spectroscopy
Abstract:
... Ultrafast two-dimensional infrared (2D-IR) spectra can now be obtained in a matter of seconds, opening up the possibility of high-throughput screening applications of relevance to the biomedical and pharmaceutical sectors. Determining quantitative information from 2D-IR spectra recorded on different samples and different instruments is however made difficult by variations in beam alignment, laser ...
DOI:
10.1021/acs.analchem.9b05601
PubMed:
31985198
PubMed Central:
PMC7145279

14. 33rd international mammalian genome conference: meeting highlights

Author:
Lauren J. Tracey; Kelsey E. Noll; Xavier Montagutelli
Source:
Mammalian genome 2020 v.31 no.3-4 pp. 69-76
ISSN:
0938-8990
Subject:
bioinformatics; epigenetics; genome; genomics; human diseases; mammals; France
Abstract:
... Scientists from 12 countries met at the International Mammalian Genome Conference (IMGC) to share advances in mammalian genetics and genomics research. The event was held in Strasbourg, France and represents the city’s second time hosting the IMGC. A diverse attendance of pre-doctoral and post-doctoral trainees, young investigators, established researchers, clinicians, bioinformaticians, and compu ...
DOI:
10.1007/s00335-020-09830-8
PubMed:
32124004
PubMed Central:
PMC7087741

15. 360-Degree Distribution of Biofilm Quantity and Community in an Operational Unchlorinated Drinking Water Distribution Pipe

Author:
Gang Liu; Ya Zhang; Xinlei Liu; Frederik Hammes; Wen-Tso Liu; Gertjan Medema; Peter Wessels; Walter van der Meer
Source:
Environmental science & technology 2020 v.54 no.9 pp. 5619-5628
ISSN:
1520-5851
Subject:
Holophaga; Hyphomicrobium; Pirellula; bacterial communities; biofilm; biomass; environmental science; sonication; water distribution
Abstract:
... In the present study, triplicate rings of 360° pipe surfaces of an operational drinking water distribution pipe were swabbed. Each ring was equally divided into 16 parts for swabbing. The collected swabs were grouped into 3 sections and compared with the biofilm samples sampled by sonication of specimens from the same pipe. The results showed that the biofilm is unevenly distributed over the 16 pa ...
DOI:
10.1021/acs.est.9b06603
PubMed:
32259432
PubMed Central:
PMC7203839

16. 3D-QSAR, docking and ADMET properties of aurone analogues as antimalarial agents

Author:
Hanine Hadni; Menana Elhallaoui
Source:
Heliyon 2020 v.6 no.4 pp. e03580
ISSN:
2405-8440
Subject:
active sites; antimalarials; aurones; cytochrome b; heme; hydrogen bonding; models; multiple drug resistance; parasites; quantitative structure-activity relationships
Abstract:
... The development of multi-resistant strains of plasmodium parasite has become a global problem, therefore, the discovery of new antimalarial agents is the only available solution. In order to improve and propose new compounds with antimalarial activity, the three-dimensional quantitative structure-activity relationship (3D-QSAR) and molecular docking studies were carried on aurone analogues acting ...
DOI:
10.1016/j.heliyon.2020.e03580
PubMed:
32322700
PubMed Central:
PMC7168746

17. 4D Genome Rewiring during Oncogene-Induced and Replicative Senescence

Author:
Satish Sati; Boyan Bonev; Quentin Szabo; Daniel Jost; Paul Bensadoun; Francois Serra; Vincent Loubiere; Giorgio Lucio Papadopoulos; Juan-Carlos Rivera-Mulia; Lauriane Fritsch; Pauline Bouret; David Castillo; Josep Ll. Gelpi; Modesto Orozco; Cedric Vaillant; Franck Pellestor; Frederic Bantignies; Marc A. Marti-Renom; David M. Gilbert; Jean-Marc Lemaitre; Giacomo Cavalli
Source:
Molecular cell 2020 v.78 no.3 pp. 522-538.e9
ISSN:
1097-2765
Subject:
DNA methylation; bisulfites; cell senescence; gene expression; genes; genomics; heterochromatin; image analysis; multiomics; sequence analysis
Abstract:
... To understand the role of the extensive senescence-associated 3D genome reorganization, we generated genome-wide chromatin interaction maps, epigenome, replication-timing, whole-genome bisulfite sequencing, and gene expression profiles from cells entering replicative senescence (RS) or upon oncogene-induced senescence (OIS). We identify senescence-associated heterochromatin domains (SAHDs). Differ ...
DOI:
10.1016/j.molcel.2020.03.007
PubMed:
32220303
PubMed Central:
PMC7208559

18. 4D Printing Elastic Composites for Strain-Tailored Multistable Shape Morphing

Author:
Heng Deng; Chi Zhang; Kianoosh Sattari; Yun Ling; Jheng-Wun Su; Zheng Yan; Jian Lin
Source:
ACS applied materials & interfaces 2020 v.13 no.11 pp. 12719-12725
ISSN:
1944-8252
Subject:
anisotropy; electronics; phase transition; three-dimensional printing
Abstract:
... Three-dimensional (3D) morphing structures with multistable shapes that can be quantitatively and reversibly altered are highly desired in many potential applications ranging from soft robots to wearable electronics. In this study, we present a 4D printing method for fabricating multistable shape-morphing structures that can be quantitatively controlled by the applied strains. The structures are p ...
DOI:
10.1021/acsami.0c17618
CHORUS:
10.1021/acsami.0c17618

19. 4D Printing of a Light-Driven Soft Actuator with Programmed Printing Density

Author:
Akihiro Nishiguchi; Hang Zhang; Sjören Schweizerhof; Marie Friederike Schulte; Ahmed Mourran; Martin Möller
Source:
ACS applied materials & interfaces 2020 v.12 no.10 pp. 12176-12185
ISSN:
1944-8252
Subject:
actuators; gels; geometry; gold; heat; manufacturing; nanogold; nanorods
Abstract:
... There is a growing interest in the concept of four-dimensional (4D) printing that combines a three-dimensional (3D) manufacturing process with dynamic modulation for bioinspired soft materials exhibiting more complex functionality. However, conventional approaches have drawbacks of low resolution, control of internal micro/nanostructure, and creation of fast, complex actuation due to a lack of hig ...
DOI:
10.1021/acsami.0c02781
PubMed:
32073253
PubMed Central:
PMC7135850

20. 53BP1 Enforces Distinct Pre- and Post-resection Blocks on Homologous Recombination

Author:
Elsa Callen; Dali Zong; Wei Wu; Nancy Wong; Andre Stanlie; Momoko Ishikawa; Raphael Pavani; Lavinia C. Dumitrache; Andrea K. Byrum; Carlos Mendez-Dorantes; Paula Martinez; Andres Canela; Yaakov Maman; Amanda Day; Michael J. Kruhlak; Maria A. Blasco; Jeremy M. Stark; Nima Mosammaparast; Peter J. McKinnon; André Nussenzweig
Source:
Molecular cell 2020 v.77 no.1 pp. 26-38.e7
ISSN:
1097-2765
Subject:
DNA damage; death; genetic instability; homologous recombination; hypersensitivity; mice; resection; single-stranded DNA
Abstract:
... 53BP1 activity drives genome instability and lethality in BRCA1-deficient mice by inhibiting homologous recombination (HR). The anti-recombinogenic functions of 53BP1 require phosphorylation-dependent interactions with PTIP and RIF1/shieldin effector complexes. While RIF1/shieldin blocks 5′-3′ nucleolytic processing of DNA ends, it remains unclear how PTIP antagonizes HR. Here, we show that mutati ...
DOI:
10.1016/j.molcel.2019.09.024
PubMed:
31653568
PubMed Central:
PMC6993210

21. 7-Acylamino-3H-1,2-benzoxathiepine 2,2-dioxides as new isoform-selective carbonic anhydrase IX and XII inhibitors

Author:
Pustenko Aleksandrs; Nocentini Alessio; Balašova Anastasija; Krasavin Mikhail; Žalubovskis Raivis; Supuran Claudiu T.
Source:
Journal of Enzyme Inhibition and Medicinal Chemistry pp. -
ISSN:
1475-6366
Subject:
arthritis; carbonate dehydratase; chemical reactions; chemotypes; chlorides; enzyme inhibition; humans; ischemia; zinc
Abstract:
... A series of 3H-1,2-benzoxathiepine 2,2-dioxides incorporating 7-acylamino moieties were obtained by an original procedure starting from 5-nitrosalicylaldehyde, which was treated with propenylsulfonyl chloride followed by Wittig reaction of the bis-olefin intermediate. The new derivatives, belonging to the homosulfocoumarin chemotype, were assayed as inhibitors of the zinc metalloenzyme carbonic an ...
Handle:
10113/7345083
DOI:
10.1080/14756366.2020.1722658
CHORUS:
10.1080/14756366.2020.1722658

22. A 450 million years long latitudinal gradient in age‐dependent extinction

Author:
Daniele Silvestro; Silvia Castiglione; Alessandro Mondanaro; Carmela Serio; Marina Melchionna; Paolo Piras; Mirko Di Febbraro; Francesco Carotenuto; Lorenzo Rook; Pasquale Raia
Source:
Ecology letters 2020 v.23 no.3 pp. 439-446
ISSN:
1461-023X
Subject:
ecology; extinction; inflation; invertebrates; risk
Abstract:
... Leigh Van Valen famously stated that under constant conditions extinction probability is independent of species age. To test this 'law of constant extinction', we developed a new method using deep learning to infer age‐dependent extinction and analysed 450 myr of marine life across 21 invertebrate clades. We show that extinction rate significantly decreases with age in > 90% of the cases, indicati ...
DOI:
10.1111/ele.13441
PubMed:
31854097
PubMed Central:
PMC7027860

23. A 5200-year paleoecological and geochemical record of coastal environmental changes and shoreline fluctuations in southwestern Louisiana: Implications for coastal sustainability

Author:
Qiang Yao; Kam-biu Liu; Alejandro Antonio Aragón-Moreno; Erika Rodrigues; Y. Jun Xu; Nina S. Lam
Source:
Geomorphology 2020 v.365 pp. 107284
ISSN:
0169-555X
Subject:
Holocene epoch; coastal ecosystems; coasts; conservation areas; fluorescence; forests; freshwater; lakes; marshes; paleoecology; palynology; pollution load; retrogradation; river deltas; rivers; sea level; sediment yield; sediments; shorelines; suspended sediment; swamps; Gulf of Mexico; Louisiana; Mississippi River
Abstract:
... This study presents a 5200-year history of coastal environmental changes and shoreline fluctuations based on a multi-proxy sedimentary record developed from the eastern end of Louisiana's Chenier Plain. Palynological, loss-on ignition, grain-size, and X-ray fluorescence data for a 525 cm sedimentary core indicate five stages of ecosystem development in the Rockefeller Wildlife Refuge since the mid ...
DOI:
10.1016/j.geomorph.2020.107284
CHORUS:
10.1016/j.geomorph.2020.107284

24. A 7-Year Trend of Malaria at Primary Health Facilities in Northwest Ethiopia

Author:
Ayenew Addisu; Yalewayker Tegegne; Yenesew Mihiret; Abebaw Setegn; Ayalew Jejaw Zeleke
Source:
Journal of parasitology research 2020 v.2020 pp. -
ISSN:
2090-0031
Subject:
Plasmodium falciparum; acidosis; autumn; chi-square distribution; computer software; control methods; cross-sectional studies; death; distress; drainage; females; health education; health services; hypoglycemia; malaria; males; nervous system; parasites; patients; rain; sampling; spring; temperature; vector control; Ethiopia
Abstract:
... Background. Malaria is a severe parasitic disease that can progress to complications of the nervous system, respiratory distress, renal problems, metabolic acidosis, and hypoglycemia which can result in death in case of delay or absence of appropriate treatment. Even though health service facilities and vector control strategy in the community are implemented as control measures, variations in tem ...
DOI:
10.1155/2020/4204987
PubMed:
32411421
PubMed Central:
PMC7204144

25. A 90 day oral toxicity study of blueberry polyphenols in ovariectomized sprague-dawley rats

Author:
Dennis P. Cladis; Shiyu Li; Lavanya Reddivari; Abigail Cox; Mario G. Ferruzzi; Connie M. Weaver
Source:
Food and chemical toxicology 2020 v.139 pp. 111254
ISSN:
0278-6915
Subject:
blueberries; body weight; chlorides; cholesterol; color; dietary supplements; food intake; fruits; histopathology; intestines; laboratory animals; monocytes; neoplasms; no observed adverse effect level; ovariectomy; pH; permeability; polyphenols; rats; urine; vegetables
Abstract:
... Regular consumption of polyphenol-rich fruits and vegetables is associated with beneficial health outcomes. To increase polyphenol intakes, consumers are increasingly using herbal and botanical dietary supplements containing concentrated polyphenol extracts. However, the safety of this consumption modality has not been vetted. To address this, ovariectomized Sprague-Dawley (OVX-SD) rats were orall ...
DOI:
10.1016/j.fct.2020.111254
PubMed:
32165232
PubMed Central:
PMC7169469

26. A 90-day dietary study with fibrillated cellulose in Sprague-Dawley rats

Author:
Kimberly J. Ong; James D. Ede; Cassidy A. Pomeroy-Carter; Christie M. Sayes; Marina R. Mulenos; Jo Anne Shatkin
Source:
Toxicology reports 2020 v.7 pp. 174-182
ISSN:
2214-7500
Subject:
blood chemistry; body weight; cellulose; diet study techniques; females; food composition; food consumption; hematology; histopathology; males; no observed adverse effect level; toxicology; urinalysis
Abstract:
... Novel forms of fibrillated cellulose offer improved attributes for use in foods. Conventional cellulose and many of its derivatives are already widely used as food additives and are authorized as safe for use in foods in many countries. However, novel forms have not yet been thoroughly investigated using standardized testing methods. This study assesses the 90-day dietary toxicity of fibrillated c ...
DOI:
10.1016/j.toxrep.2020.01.003
CHORUS:
10.1016/j.toxrep.2020.01.003

27. A Bayesian approach to joint modeling of matrix‐valued imaging data and treatment outcome with applications to depression studies

Author:
Bei Jiang; Eva Petkova; Thaddeus Tarpey; R. Todd Ogden
Source:
Biometrics 2020 v.76 no.1 pp. 87-97
ISSN:
0006-341X
Subject:
Bayesian theory; biometry; electroencephalography; image analysis; models; prediction; principal component analysis
Abstract:
... In this paper, we propose a unified Bayesian joint modeling framework for studying association between a binary treatment outcome and a baseline matrix‐valued predictor. Specifically, a joint modeling approach relating an outcome to a matrix‐valued predictor through a probabilistic formulation of multilinear principal component analysis is developed. This framework establishes a theoretical relati ...
DOI:
10.1111/biom.13151
PubMed:
31529701
PubMed Central:
PMC7067625

28. A Cas12a ortholog with stringent PAM recognition followed by low off-target editing rates for genome editing

Author:
Peng Chen; Jin Zhou; Yibin Wan; Huan Liu; Yongzheng Li; Zhaoxin Liu; Hongjian Wang; Jun Lei; Kai Zhao; Yiliang Zhang; Yan Wang; Xinghua Zhang; Lei Yin
Source:
Genome biology 2020 v.21 no.1 pp. 78
ISSN:
1474-760X
Subject:
Coprococcus eutactus; gene editing; humans; nucleases; therapeutics
Abstract:
... BACKGROUND: AsCas12a and LbCas12a nucleases are reported to be promising tools for genome engineering with protospacer adjacent motif (PAM) TTTV as the optimal. However, the C-containing PAM (CTTV, TCTV, TTCV, etc.) recognition by Cas12a might induce extra off-target edits at these non-canonical PAM sites. RESULTS: Here, we identify a novel Cas12a nuclease CeCas12a from Coprococcus eutactus, which ...
DOI:
10.1186/s13059-020-01989-2
PubMed:
32213191
PubMed Central:
PMC7093978

29. A Case Study of Cimex lectularius L. (Hemiptera: Cimicidae) Infestations in an Office Environment

Author:
Shannon Sked; Changlu Wang; Michael Levy; Kathryn Hacker
Source:
Journal of economic entomology 2020 v.112 no.4 pp. 1821-1830
ISSN:
0022-0493
Subject:
Cimex lectularius; case studies; dry ice; dust; entomology; insecticides; silica; working conditions
Abstract:
... Most field studies on Cimex lectularius are conducted in residential or hospitality industrial settings. Cimex lectularius infestations in office settings are reported, but are rarely studied. An office environment (~875 m²) consisting of 105 cubicles or pod-seating areas with persistent C. lectularius sightings over a 2-yr period was evaluated for 90 d through intensive trapping to determine C. l ...
DOI:
10.1093/jee/toz108
CHORUS:
10.1093/jee/toz108

30. A Classical Molecular Dynamics Simulation Study of Interfacial and Bulk Solution Aggregation Properties of Dirhamnolipids

Author:
Charles M. Luft; Elango Munusamy; Jeanne E. Pemberton; Steven D. Schwartz
Source:
Journal of physical chemistry 2020 v.124 no.5 pp. 814-827
ISSN:
1520-5207
Subject:
Pseudomonas aeruginosa; bacteria; biodegradability; biosurfactants; hydrophilicity; hydrophobicity; liquid-air interface; molecular dynamics; molecular weight; rhamnolipids; rhamnose; surface area
Abstract:
... The rhamnolipids are a unique class of biosurfactants produced by the bacteria Pseudomonas aeruginosa. These molecules display a high level of surface activity as well as biodegradability. In this study nonionic dirhamnolipid was investigated by utilizing molecular dynamics simulation at the air–water interface as well as in bulk water. Detailed structural analysis is presented for both the interf ...
DOI:
10.1021/acs.jpcb.9b08800
PubMed:
31958226
PubMed Central:
PMC7042949

31. A Co-orientation Analysis of Producers’ and Environmentalists’ Mental Models of Water Issues: Opportunities for Improved Communication and Collaboration

Author:
Hundemer Sadie; Monroe Martha C.
Source:
Environmental Communication pp. -
ISSN:
1752-4032
Subject:
agricultural industry; cognition; ethics; models; stakeholders
Abstract:
... Agricultural producers and environmentalists have a mutual interest in maintaining healthy water resources; yet communication and collaboration on sustainability measures can be impeded by perceptions of incompatible water ethics. Here we apply the co-orientation model to qualitatively and quantitatively compare cross-group perspectives and composite maps of stakeholders’ mental models to understa ...
Handle:
10113/7612771
DOI:
10.1080/17524032.2020.1828128
CHORUS:
10.1080/17524032.2020.1828128

32. A Computational Protocol for Regulating Protein Binding Reactions with a Light-Sensitive Protein Dimer

Author:
Frank D. Teets; Takashi Watanabe; Klaus M. Hahn; Brian Kuhlman
Source:
Journal of molecular biology 2020 v.432 no.4 pp. 805-814
ISSN:
0022-2836
Subject:
animals; blue light; guanosinetriphosphatase; irradiation; molecular models; optogenetics; protein binding; proteins
Abstract:
... Light-sensitive proteins can be used to perturb signaling networks in living cells and animals with high spatiotemporal resolution. We recently engineered a protein heterodimer that dissociates when irradiated with blue light and demonstrated that by fusing each half of the dimer to termini of a protein that it is possible to selectively block binding surfaces on the protein when in the dark. On a ...
DOI:
10.1016/j.jmb.2019.12.033
PubMed:
31887287
PubMed Central:
PMC7054171

33. A Concise, Enantioselective Approach for the Synthesis of Yohimbine Alkaloids

Author:
Eric R. Miller; M. Todd Hovey; Karl A. Scheidt
Source:
Journal of the American Chemical Society 2020 v.142 no.5 pp. 2187-2192
ISSN:
1520-5126
Subject:
catalytic activity; diastereoselectivity; dimerization; enantiomers; enantioselective synthesis; enantioselectivity; yohimbine
Abstract:
... We report a concise, enantioselective synthesis of the yohimbine alkaloids (−)-rauwolscine and (−)-alloyohimbane. The key transformation involves a highly enantio- and diastereoselective NHC-catalyzed dimerization and an amidation/N-acyliminium ion cyclization sequence to furnish four of the five requisite rings and three of the five stereocenters in two operations. This route also provides effici ...
DOI:
10.1021/jacs.9b12319
PubMed:
31951394
PubMed Central:
PMC7185878

34. A Conserved Noncoding Locus Regulates Random Monoallelic Xist Expression across a Topological Boundary

Author:
Rafael Galupa; Elphège Pierre Nora; Rebecca Worsley-Hunt; Christel Picard; Chris Gard; Joke Gerarda van Bemmel; Nicolas Servant; Yinxiu Zhan; Fatima El Marjou; Colin Johanneau; Patricia Diabangouaya; Agnès Le Saux; Sonia Lameiras; Juliana Pipoli da Fonseca; Friedemann Loos; Joost Gribnau; Sylvain Baulande; Uwe Ohler; Luca Giorgetti; Edith Heard
Source:
Molecular cell 2020 v.77 no.2 pp. 352-367.e8
ISSN:
1097-2765
Subject:
X chromosome; genome; loci; mammals; non-coding RNA
Abstract:
... cis-Regulatory communication is crucial in mammalian development and is thought to be restricted by the spatial partitioning of the genome in topologically associating domains (TADs). Here, we discovered that the Xist locus is regulated by sequences in the neighboring TAD. In particular, the promoter of the noncoding RNA Linx (LinxP) acts as a long-range silencer and influences the choice of X chr ...
DOI:
10.1016/j.molcel.2019.10.030
PubMed:
31759823
PubMed Central:
PMC6964159

35. A Convolutional Neural Network-Based Approach for the Rapid Annotation of Molecularly Diverse Natural Products

Author:
Raphael Reher; Hyun Woo Kim; Chen Zhang; Huanru Henry Mao; Mingxun Wang; Louis-Félix Nothias; Andres Mauricio Caraballo-Rodriguez; Evgenia Glukhov; Bahar Teke; Tiago Leao; Kelsey L. Alexander; Brendan M. Duggan; Ezra L. Van Everbroeck; Pieter C. Dorrestein; Garrison W. Cottrell; William H. Gerwick
Source:
Journal of the American Chemical Society 2020 v.142 no.9 pp. 4114-4120
ISSN:
1520-5126
Subject:
Symploca; artificial intelligence; cyclic peptides; cytotoxicity; drugs; fractionation; macrolides; neoplasm cells; neoplasms; nuclear magnetic resonance spectroscopy
Abstract:
... This report describes the first application of the novel NMR-based machine learning tool “Small Molecule Accurate Recognition Technology” (SMART 2.0) for mixture analysis and subsequent accelerated discovery and characterization of new natural products. The concept was applied to the extract of a filamentous marine cyanobacterium known to be a prolific producer of cytotoxic natural products. This ...
DOI:
10.1021/jacs.9b13786
PubMed:
32045230
PubMed Central:
PMC7210566

36. A Covalent and Modular Synthesis of Homo- and Hetero[n]rotaxanes

Author:
Milo D. Cornelissen; Simone Pilon; Luuk Steemers; Martin J. Wanner; Steven Frölke; Ed Zuidinga; Steen Ingemann Jørgensen; Jarl Ivar van der Vlugt; Jan H. van Maarseveen
Source:
Journal of organic chemistry 2020 v.85 no.5 pp. 3146-3159
ISSN:
1520-6904
Subject:
alkynes; azides; chemical reactions; chemical structure; organic chemistry; rotaxanes
Abstract:
... Incorporation of 2,5-dihydroxyterephthalate as a covalent scaffold in the axis of a 30-membered all-carbon macrocycle provides access to a modular series of rotaxanes. Installment of tethered alkynes or azides onto the terephthalic phenolic hydroxyl functionalities, which are situated at opposite sides of the macrocycle, gives versatile prerotaxane building blocks. The corresponding [2]rotaxanes a ...
DOI:
10.1021/acs.joc.9b03030
PubMed:
31965801
PubMed Central:
PMC7063575

37. A Critical Review on Tropical Fruits Seeds as Prospective Sources of Nutritional and Bioactive Compounds for Functional Foods Development: A Case of Indonesian Exotic Fruits

Author:
Andri Cahyo Kumoro; Misbahudin Alhanif; Dyah Hesti Wardhani
Source:
International Journal of Food Sciences 2020 v.2020 pp. -
ISSN:
2314-5765
Subject:
Salacca zalacca; antioxidant activity; avocados; bioactive compounds; bioavailability; byproducts; carbohydrates; carotenoids; durians; flavonoids; fruits; functional foods; functional properties; introduced plants; jackfruits; mangoes; marketing; oranges; phenolic acids; polyphenols; powders; protein content; rambutans; seeds; social welfare; trans fatty acids; South East Asia
Abstract:
... Some tropical countries in the Southeast Asia are rich in exotic fruits with worldwide acceptance, such as mango, orange, snake fruit, durian, jackfruit, rambutan, and avocado. In addition to their abundant production, those fruits are also currently gaining expansive distribution and marketing due to their promising advantages to human well-being. Surprisingly, their by-products, especially the s ...
DOI:
10.1155/2020/4051475
PubMed:
32258095
PubMed Central:
PMC7103992

38. A Deleted Deletion Site in a New Vector Strain and Exceptional Genomic Stability of Plaque-Purified Modified Vaccinia Ankara (MVA)

Author:
Ingo Jordan; Deborah Horn; Kristin Thiele; Lars Haag; Katharina Fiddeke; Volker Sandig
Source:
Virologica Sinica 2020 v.35 no.2 pp. 212-226
ISSN:
1674-0769
Subject:
cell lines; chick embryos; cost effectiveness; fibroblasts; genetic stability; genomics; genotype; giant cells; immunity; phenotype; point mutation; regulator genes; transgenes; vaccines
Abstract:
... Vectored vaccines based on highly attenuated modified vaccinia Ankara (MVA) are reported to be immunogenic, tolerant to pre-existing immunity, and able to accommodate and stably maintain very large transgenes. MVA is usually produced on primary chicken embryo fibroblasts, but production processes based on continuous cell lines emerge as increasingly robust and cost-effective alternatives. An isola ...
DOI:
10.1007/s12250-019-00176-3
PubMed:
31833037
PubMed Central:
PMC7198643

39. A Dermonutrient Containing Special Collagen Peptides Improves Skin Structure and Function: A Randomized, Placebo-Controlled, Triple-Blind Trial Using Confocal Laser Scanning Microscopy on the Cosmetic Effects and Tolerance of a Drinkable Collagen Supplement

Author:
Sabrina Laing; Stephan Bielfeldt; Carolin Ehrenberg; Klaus-Peter Wilhelm
Source:
Journal of medicinal food 2020 v.23 no.2 pp. 147-152
ISSN:
1557-7600
Subject:
acerolas; ascorbic acid; biotin; collagen; dietary supplements; females; fruit extracts; health status; image analysis; oral exposure; peptides; randomized clinical trials; skin (animal); vitamin E; volunteers; zinc
Abstract:
... The purpose of this randomized, placebo-controlled, triple-blind trial on 60 healthy female volunteers was to assess the cosmetic effects on skin quality of a food supplement containing special collagen peptides together with acerola extract, vitamin C, vitamin E, biotin, and zinc after an intake of 12 weeks (Elasten®, QUIRIS Healthcare, Germany). To reduce assessment bias maximally and increase t ...
DOI:
10.1089/jmf.2019.0197
PubMed:
32017646
PubMed Central:
PMC7041324

40. A Digital Health Weight Loss Program in 250,000 Individuals

Author:
Conor Senecal; Robert Jay Widmer; Beth R. Larrabee; Mariza de Andrade; Lilach O. Lerman; Amir Lerman; Francisco Lopez-Jimenez
Source:
Journal of obesity 2020 v.2020 pp. -
ISSN:
2090-0716
Subject:
females; men; obesity; observational studies; weight control; weight loss; women; China
Abstract:
... Importance. Obesity is a worsening epidemic worldwide. Effective and accessible weight loss programs to combat obesity on a large scale are warranted, but a need for frequent face-to-face care might impose a limitation. Objective. To evaluate whether individuals following a weight loss program based on a mobile application, wireless scale, and nutritional program but no face-to-face care can achie ...
DOI:
10.1155/2020/9497164
PubMed:
32300485
PubMed Central:
PMC7136816

41. A Dinuclear Ruthenium(II) Complex Excited by Near-Infrared Light through Two-Photon Absorption Induces Phototoxicity Deep within Hypoxic Regions of Melanoma Cancer Spheroids

Author:
Ahtasham Raza; Stuart A. Archer; Simon D. Fairbanks; Kirsty L. Smitten; Stanley W. Botchway; James A. Thomas; Sheila MacNeil; John W. Haycock
Source:
Journal of the American Chemical Society 2020 v.142 no.10 pp. 4639-4647
ISSN:
1520-5126
Subject:
DNA; cell culture; cell death; emissions factor; human diseases; irradiation; luminescence; melanoma; mitochondria; models; near infrared radiation; neoplasm cells; phototherapy; phototoxicity; ruthenium; singlet oxygen
Abstract:
... The dinuclear photo-oxidizing Ruᴵᴵ complex [{Ru(TAP₂)}₂(tpphz)]⁴⁺ (TAP = 1,4,5,8- tetraazaphenanthrene, tpphz = tetrapyrido[3,2-a:2′,3′-c:3″,2′′-h:2‴,3′′′-j]phenazine), 1⁴⁺, is readily taken up by live cells localizing in mitochondria and nuclei. In this study, the two-photon absorption cross section of 1⁴⁺ is quantified and its use as a two-photon absorbing phototherapeutic is reported. It was co ...
DOI:
10.1021/jacs.9b11313
PubMed:
32065521
PubMed Central:
PMC7146853

42. A Five-Genes-Based Prognostic Signature for Cervical Cancer Overall Survival Prediction

Author:
Menghuang Zhao; Wenbin Huang; Shuangwei Zou; Qi Shen; Xueqiong Zhu
Source:
International Journal of Genomics 2020 v.2020 pp. -
ISSN:
2314-4378
Subject:
DNA replication; adenocarcinoma; carcinogenesis; cell cycle; chi-square distribution; data collection; databases; gene expression; gene expression regulation; genomics; histology; lymph nodes; metastasis; models; patients; prediction; risk; uterine cervical neoplasms
Abstract:
... Aims. This study is aimed at identifying a prognostic signature for cervical cancer. Main Methods. The gene expression data and clinical information of cervical cancer and normal cervical tissues were acquired from The Cancer Genome Atlas and from three datasets of the Gene Expression Omnibus database. DESeq2 and Limma were employed to screen differentially expressed genes (DEGs). The overlapping ...
DOI:
10.1155/2020/8347639
PubMed:
32300605
PubMed Central:
PMC7136791

43. A Genome-wide ER-phagy Screen Highlights Key Roles of Mitochondrial Metabolism and ER-Resident UFMylation

Author:
Jin Rui Liang; Emily Lingeman; Thao Luong; Saba Ahmed; Matthias Muhar; Truc Nguyen; James A. Olzmann; Jacob E. Corn
Source:
Cell 2020 v.180 no.6 pp. 1160-1177.e20
ISSN:
0092-8674
Subject:
cell differentiation; homeostasis; human diseases; humans; ligases; mitochondria; mitophagy; peripheral nervous system diseases; receptors; screening; unfolded protein response
Abstract:
... Selective autophagy of organelles is critical for cellular differentiation, homeostasis, and organismal health. Autophagy of the ER (ER-phagy) is implicated in human neuropathy but is poorly understood beyond a few autophagosomal receptors and remodelers. By using an ER-phagy reporter and genome-wide CRISPRi screening, we identified 200 high-confidence human ER-phagy factors. Two pathways were une ...
DOI:
10.1016/j.cell.2020.02.017
PubMed:
32160526
PubMed Central:
PMC7197389

44. A Genomic Perspective on the Origin and Emergence of SARS-CoV-2

Author:
Yong-Zhen Zhang; Edward C. Holmes
Source:
Cell 2020 v.181 no.2 pp. 223-227
ISSN:
0092-8674
Subject:
animal viruses; genomics; pandemic; sequence analysis; viruses; China
Abstract:
... The ongoing pandemic of a new human coronavirus, SARS-CoV-2, has generated enormous global concern. We and others in China were involved in the initial genome sequencing of the virus. Herein, we describe what genomic data reveal about the emergence SARS-CoV-2 and discuss the gaps in our understanding of its origins. ...
DOI:
10.1016/j.cell.2020.03.035
PubMed:
32220310
PubMed Central:
PMC7194821

45. A Glimpse into the Microbiota of Marketed Ready-to-Eat Crickets (Acheta domesticus)

Author:
Vesna Milanović; Federica Cardinali; Lucia Aquilanti; Cristiana Garofalo; Andrea Roncolini; Riccardo Sabbatini; Francesca Clementi; Andrea Osimani
Source:
Indian journal of microbiology 2020 v.60 no.1 pp. 115-118
ISSN:
0046-8991
Subject:
Acheta domesticus; Clostridium; European Union; Hydrogenophilus; Lysobacter; Pseudomonas; Ruminococcus; Staphylococcus; bacteria; commercialization; denaturing gradient gel electrophoresis; enzymes; genes; insects; polymerase chain reaction; ready-to-eat foods; rearing; ribosomal RNA; Austria; Belgium; France; Netherlands
Abstract:
... The present study was aimed to get an insight into the bacterial biota of ready-to-eat small crickets (Acheta domesticus) already marketed in the European Union. 16S rRNA gene of the DNAs extracted from thirty-two samples of ready-to-eat crickets commercialized by 4 European Union producers located in Austria, Belgium, France and the Netherlands (2 batches per producer) was analyzed by Polymerase ...
DOI:
10.1007/s12088-019-00817-x
PubMed:
32089581
PubMed Central:
PMC7000585

46. A Guide and Toolbox to Replicability and Open Science in Entomology

Author:
Jacob T. Wittman; Brian H. Aukema
Source:
Journal of Insect Science 2020 v.20 no.3 pp. -
ISSN:
1536-2442
Subject:
entomology; information management; insects; medicine; quality control
Abstract:
... The ability to replicate scientific experiments is a cornerstone of the scientific method. Sharing ideas, workflows, data, and protocols facilitates testing the generalizability of results, increases the speed that science progresses, and enhances quality control of published work. Fields of science such as medicine, the social sciences, and the physical sciences have embraced practices designed t ...
Handle:
10113/7367977
DOI:
10.1093/jisesa/ieaa036
CHORUS:
10.1093/jisesa/ieaa036

47. A Highly Sensitive and Selective Isobutyraldehyde Sensor Based on Nanosized Sm2O3 Particles

Author:
Li Jiang; Yun Wu; Yan Wang; Qin Zhou; Yuguo Zheng; Yafei Chen; Qianchun Zhang
Source:
Journal of Analytical Methods in Chemistry (Online) 2020 v.2020 pp. -
ISSN:
2090-8873
Subject:
acetaldehyde; acetone; benzene; detection limit; durability; ethanol; ethylbenzene; formaldehyde; methanol; nanomaterials; signal-to-noise ratio; standard deviation; temperature; wavelengths
Abstract:
... A highly sensitive and selective sensor for isobutyraldehyde (IBD) is demonstrated based on intensive cataluminescence (CTL) emission from the surface of nanosized Sm₂O₃ particles. The characteristics and optimum conditions for the CTL sensor, including the working temperature, wavelength, and flow rate, were investigated in detail. Under the optimized experimental conditions, the CTL intensity va ...
DOI:
10.1155/2020/5205724
PubMed:
32322424
PubMed Central:
PMC7152939

48. A Ln(iii)-3-hydroxypyridine pH responsive probe optimized by DFT

Author:
Michael A. Caldwell; Christopher R. Brue; Tyler J. Whittemore; Thomas J. Meade
Source:
RSC advances 2020 v.10 no.15 pp. 8994-8999
ISSN:
2046-2069
Subject:
fluorescence; lanthanides; ligands; magnetism; metabolism; pH; protonation
Abstract:
... Differences in tissue pH can be diagnostic of cancer and other conditions that shift cell metabolism. Paramagnetic probes are promising tools for pH mapping in vivo using magnetic resonance spectroscopy (MRS) as they provide uniquely shifted MR signals that change with pH. Here, we demonstrate a 3-hydroxy-6-methylpyridyl coordinating group as a new pH-responsive reporter group for Ln(iii) MRS prob ...
DOI:
10.1039/c9ra11058e
PubMed:
32274014
PubMed Central:
PMC7144623

49. A LuxR‐type regulator, AcrR, regulates flagellar assembly and contributes to virulence, motility, biofilm formation, and growth ability of Acidovorax citrulli

Author:
Wei Guan; Tielin Wang; Qi Huang; Eryuan Tian; Bo Liu; Yuwen Yang; Tingchang Zhao
Source:
Molecular plant pathology 2020 v.21 no.4 pp. 489-501
ISSN:
1464-6722
Subject:
Acidovorax avenae subsp. citrulli; bacterial motility; biofilm; fruits; gene expression regulation; genes; mutants; pathogens; transcriptomics; virulence; watermelons
Abstract:
... LuxR‐type regulators regulate many bacterial processes and play important roles in bacterial motility and virulence. Acidovorax citrulli is a seedborne bacterial pathogen responsible for bacterial fruit blotch, which causes great losses in melon and watermelon worldwide. We identified a LuxR‐type, nonquorum sensing‐related regulator, AcrR, in the group II strain Aac‐5 of A. citrulli. We found that ...
DOI:
10.1111/mpp.12910
PubMed:
31943660
PubMed Central:
PMC7060138

50. A Mechanism to Minimize Errors during Non-homologous End Joining

Author:
Benjamin M. Stinson; Andrew T. Moreno; Johannes C. Walter; Joseph J. Loparo
Source:
Molecular cell 2020 v.77 no.5 pp. 1080-1091.e8
ISSN:
1097-2765
Subject:
DNA; DNA damage; DNA repair; catalytic activity; eggs; frogs; image analysis; mutagenesis; mutagens; nucleases
Abstract:
... Enzymatic processing of DNA underlies all DNA repair, yet inappropriate DNA processing must be avoided. In vertebrates, double-strand breaks are repaired predominantly by non-homologous end joining (NHEJ), which directly ligates DNA ends. NHEJ has the potential to be highly mutagenic because it uses DNA polymerases, nucleases, and other enzymes that modify incompatible DNA ends to allow their liga ...
DOI:
10.1016/j.molcel.2019.11.018
PubMed:
31862156
PubMed Central:
PMC7060804