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You searched for: Publication Year 2019 Remove constraint Publication Year: 2019 Source 2019 v.164 no.5 Remove constraint Source: 2019 v.164 no.5 Subject viruses Remove constraint Subject: viruses
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1. A rapid, sensitive and inexpensive method for detection of grapevine red blotch virus without tissue extraction using loop-mediated isothermal amplification

Author:
Romero Romero, J. Lucina; Carver, Gavriela Dena; Arce Johnson, Patricio; Perry, Keith L.; Thompson, Jeremy R.
Source:
Archives of virology 2019 v.164 no.5 pp. 1453-1457
ISSN:
0304-8608
Subject:
Grapevine red blotch virus; loop-mediated isothermal amplification; nucleic acids; polymerase chain reaction; viruses; viticulture; North America
Abstract:
... Grapevine red blotch virus (GRBV) is an emerging virus of significant viticultural importance throughout North America. Here, we report the development of a simple protocol for point-of-use detection of GRBV. Extraction of nucleic acids is not required; instead, the whole intact plant can simply be pricked with a sterile pipette tip, which is then incubated in sterile distilled water to provide th ...
DOI:
10.1007/s00705-019-04207-y

2. Coinfection of a lingual lesion with bovine papular stomatitis virus and bovine papillomavirus

Author:
Zhu, Wei; Haga, Takeshi; Yuan, Dongfang; Watanabe, Ken-ichi; Chambers, James K.; Gao, Nannan; Uchida, Kazuyuki; Fan, Shushan; Li, Fang; Yang, Mingcai; Norimine, Junzo; Hu, Shilin; Dong, Jianbao
Source:
Archives of virology 2019 v.164 no.5 pp. 1441-1444
ISSN:
0304-8608
Subject:
Bovine papular stomatitis virus; Papillomaviridae; cows; epidemiological studies; histopathology; immunohistochemistry; mixed infection; papilloma; reverse transcriptase polymerase chain reaction; slaughterhouses; tongue; viruses
Abstract:
... To date, there have been no reports of coinfection with bovine papular stomatitis virus (BPSV) and bovine papillomavirus (BPV) in the same lesion. In the present study, one lingual papilloma-like sample was collected at an abattoir from the tongue of a 31-month-old Japanese black cow. Coinfection with BPSV and BPV was confirmed by histopathology, immunohistochemistry, PCR and RT-PCR. The evidence ...
DOI:
10.1007/s00705-019-04199-9

3. Establishment of a rescue system for porcine parvovirus using a seamless cloning method

Author:
Zhang, Lingling; Gao, Dou; Yu, Yongle; Liu, Yebing; Liu, Weiquan; Li, Jinxiang; Cui, Shangjin
Source:
Archives of virology 2019 v.164 no.5 pp. 1459-1467
ISSN:
0304-8608
Subject:
Ungulate protoparvovirus 1; genetic markers; pathogenesis; rapid methods; viruses
Abstract:
... In this study, we describe a novel and rapid method for the construction of a full-length infectious clone (pPPV). The constructed clone contained an engineered EcoRv site that served as a genetic marker and was shown to be infectious when transfected into a monolayer of PK-15 cells. The rescued virus (rPPV) of the infectious clone was found to be indistinguishable from wild-type virus BQ in terms ...
DOI:
10.1007/s00705-019-04209-w

4. Genomic characterization of a novel recombinant porcine astrovirus isolated in northeastern China

Author:
Zhao, Chengxue; Chen, Chen; Li, Ying; Dong, Shuren; Tan, Kun; Tian, Yuhui; Zhang, Lilin; Huang, Jinhai; Zhang, Lei
Source:
Archives of virology 2019 v.164 no.5 pp. 1469-1473
ISSN:
0304-8608
Subject:
Mamastrovirus 3; amino acid sequences; coat proteins; diarrhea; evolution; feces; genetic recombination; genetic variation; genome; genomics; herds; piglets; promoter regions; reverse transcriptase polymerase chain reaction; sequence analysis; viruses; China
Abstract:
... Porcine astroviruses (PAstVs), are widely distributed viruses that are highly prevalent in swine herds. In this study, a novel type 4 porcine astrovirus strain (designated as PAstV4/Tianjin/2018) was identified in a fecal sample from a diarrheal piglet in Tianjin, China and its complete genomic sequence was determined by RT-PCR. Sequence analysis showed that this strain had a capsid protein with a ...
DOI:
10.1007/s00705-019-04162-8

5. Induction of neutralizing antibodies against tier 2 human immunodeficiency virus 1 in rhesus macaques infected with tier 1B simian/human immunodeficiency virus

Author:
Himeno, Ai; Ishida, Yuki; Mori, Hiromi; Matsuura, Kanako; Kikukawa, Minako; Sakawaki, Hiromi; Miura, Tomoyuki
Source:
Archives of virology 2019 v.164 no.5 pp. 1297-1308
ISSN:
0304-8608
Subject:
CCR5 receptor; CXCR4 receptor; Human immunodeficiency virus 1; Macaca mulatta; clones; models; mutation; mutational analysis; neutralization; neutralizing antibodies; vaccines; viruses
Abstract:
... We previously developed CCR5-tropic neutralization-resistant simian/human immunodeficiency virus (SHIV) strains and a rhesus macaque model of infection with these SHIVs. We induced the production of neutralizing antibodies (nAbs) against HIV-1 by infecting rhesus macaques with different neutralization-resistant SHIV strains. First, SHIV-MK1 (MK1) (neutralization susceptible, tier 1B) with CCR5 tro ...
DOI:
10.1007/s00705-019-04173-5

6. L233P mutation in the bovine leukemia virus Tax protein depresses endothelial cell recruitment and tumorigenesis in athymic nude mice

Author:
Mori, Hiroshi; Tomiyasu, Takafumi; Nishiyama, Kanako; Matsumoto, Maiko; Osawa, Yoshiaki; Okazaki, Katsunori
Source:
Archives of virology 2019 v.164 no.5 pp. 1343-1351
ISSN:
0304-8608
Subject:
Bovine leukemia virus; amino acids; angiogenesis; blood vessels; carcinogenesis; cattle; cell lines; chemoattractants; chemotaxis; fibroblasts; human umbilical vein endothelial cells; mice; mutation; neoplasm cells; neoplasms; rats; stem cells; viruses
Abstract:
... Bovine leukemia virus (BLV) can be divided into two categories based on the amino acid at position 233 in the Tax protein, which probably plays a crucial role in leukemogenesis. We show here that a rat fibroblast cell line stably expressing L233-Tax formed significantly larger tumors than P233-Tax-expressing cells in a murine xenograft study. Although the microvessel density was comparable in both ...
DOI:
10.1007/s00705-019-04191-3

7. Molecular characterization of a new botybirnavirus that infects Botrytis cinerea

Author:
Cottet, Luis; Potgieter, Christiaan A.; Castro, Miguel E.; Castillo, Antonio
Source:
Archives of virology 2019 v.164 no.5 pp. 1479-1483
ISSN:
0304-8608
Subject:
Alternaria; Botrytis cinerea; RNA-directed RNA polymerase; amino acid sequences; double-stranded RNA; electrophoresis; fungi; genome; nucleotides; open reading frames; phylogeny; polypeptides; sequence analysis; viruses
Abstract:
... Eight different double-stranded RNA (dsRNA) molecules were found in the wild-type fungal strain Botrytis cinerea CCg427. The electrophoretic profile displayed molecules with approximate sizes of 1, 1.3, 1.6, 1.8, 3.3, 4.1, 6.5, and 12 kbp. Sequences analysis of the molecules in the 6.5-kbp band revealed the presence of two different dsRNA molecules (dsRNA-1 and dsRNA-2) of 6192 and 5567 bp. Each m ...
DOI:
10.1007/s00705-019-04184-2

8. New variants of canine parvovirus in dogs in southern Brazil

Author:
de Oliveira, Pablo Sebastian Britto; Cargnelutti, Juliana Felipetto; Masuda, Eduardo Kenji; Weiblen, Rudi; Flores, Eduardo Furtado
Source:
Archives of virology 2019 v.164 no.5 pp. 1361-1369
ISSN:
0304-8608
Subject:
Carnivore protoparvovirus 1; amino acid sequences; amino acids; coat proteins; dog diseases; dogs; genetic markers; immune system; mutation; phylogeny; receptors; sequence analysis; vaccines; viruses; Brazil
Abstract:
... Carnivore protoparvovirus 1 (canine parvovirus 2, CPV-2) has undergone a rapid evolution through mutations in the capsid protein VP2, giving rise to variants associated with unique clinicopathological and immunological features. VP2 is a major capsid protein involved in key steps of virus biology, including interactions with cellular receptors and with the immune system. This study analyzed the co ...
DOI:
10.1007/s00705-019-04198-w

9. Novel bird’s-foot trefoil RNA viruses provide insights into a clade of legume-associated enamoviruses and rhabdoviruses

Author:
Debat, Humberto J.; Bejerman, Nicolas
Source:
Archives of virology 2019 v.164 no.5 pp. 1419-1426
ISSN:
0304-8608
Subject:
Enamovirus; Lotus corniculatus; Nucleorhabdovirus; genes; monophyly; plant viruses; transcriptome; viruses
Abstract:
... Here, we report the identification and characterization of two novel viruses associated with bird’s-foot trefoil. Virus sequences related to those of enamoviruses (ssRNA (+); Luteoviridae; Enamovirus) and nucleorhabdoviruses (ssRNA (-); Rhabdoviridae; Nucleorhabdovirus) were detected in Lotus corniculatus transcriptome data. The genome of the tentatively named “bird’s-foot trefoil-associated virus ...
DOI:
10.1007/s00705-019-04193-1

10. Porcine circovirus type 2 ORF5 protein induces endoplasmic reticulum stress and unfolded protein response in porcine alveolar macrophages

Author:
Ouyang, Yueling; Xu, Lei; Lv, Jiangman; Hou, Yufeng; Fan, Zhixin; Xu, Panpan; Jiang, Yanfen; Wu, Mengmeng; Li, Ran; Zhang, Yanming; Guo, Kangkang
Source:
Archives of virology 2019 v.164 no.5 pp. 1323-1334
ISSN:
0304-8608
Subject:
Porcine circovirus; Porcine circovirus-2; Western blotting; apoptosis; coat proteins; endoplasmic reticulum; endoplasmic reticulum stress; enzymes; industry; inositols; macrophages; open reading frames; pathogenesis; pathogens; quantitative polymerase chain reaction; signal transduction; swine; transcription factors; transmission electron microscopy; unfolded protein response; viral nonstructural proteins; viruses
Abstract:
... Porcine circovirus type 2 (PCV2) is the essential infectious agent causing porcine circovirus-associated disease (PCVD) in pigs and one of the important viruses that severely jeopardize the swine husbandry industry. PCV2 elicits the unfolded protein response (UPR) via activation of the PERK pathway, and its capsid protein (Cap) has also been found to induce UPR with subsequent activation of apopto ...
DOI:
10.1007/s00705-019-04185-1

11. Preparation of a monoPEGylated derivative of cyanovirin-N and its virucidal effect on acyclovir-resistant strains of herpes simplex virus type 1

Author:
Lei, Yun; Chen, Wei; Liang, Hanzhang; Wang, Zhihua; Chen, Jia; Hong, Hui; Xie, Long; Nie, Hong; Xiong, Sheng
Source:
Archives of virology 2019 v.164 no.5 pp. 1259-1269
ISSN:
0304-8608
Subject:
Human alphaherpesvirus 1; antiviral agents; antiviral properties; chemical bonding; drug resistance; immunogenicity; inhibitory concentration 50; polyethylene glycol; therapeutics; toxicity; viruses
Abstract:
... The long-term administration of acyclovir (ACV) for therapy against herpes simplex virus type 1 (HSV-1) infections can result in the emergence of ACV-resistant HSV strains. It is therefore urgent to develop new anti-herpetic compounds with mechanisms that differ from that of ACV. Cyanovirin-N (CV-N) is an antiviral agent that has an inhibitory effect on HSV-1 infections, and PEGylation of CV-N is ...
DOI:
10.1007/s00705-018-04118-4

12. Rescue and characterization of a recombinant HY12 bovine enterovirus carrying a foreign HA epitope in the 3A nonstructural protein

Author:
Liu, Dan; Liu, Changming; Liu, Xing; Li, Xin; Huang, Liping; Hu, Junying; Wei, Yanwu; Zhu, Hongzhen; Zhang, Qun; Wang, Xinping
Source:
Archives of virology 2019 v.164 no.5 pp. 1309-1321
ISSN:
0304-8608
Subject:
Enterovirus; Orthomyxoviridae; Western blotting; amino acids; antibodies; cell lines; clones; complementary DNA; electron microscopy; epitopes; genetic markers; hemagglutinins; immunoperoxidase monolayer assay; mice; nucleotide sequences; pathogenesis; pathogenicity; vaccine development; viral nonstructural proteins; virus replication; viruses
Abstract:
... Full-length infectious cDNA clones for recombinant HY12 bovine enteroviruses designated as rHY12-3A-2-HA, rHY12-3A-3-HA, and rHY12-3A-9-HA were constructed by the insertion of an epitope from influenza virus hemagglutinin (HA) at the N-terminus of the HY12-encoded 3A protein at amino acid positions 2, 3, and 9. The recombinant HY12 viruses expressing the HA epitope were rescued and characterized u ...
DOI:
10.1007/s00705-019-04178-0

13. The complete nucleotide sequence and genome organization of a novel virus of the order Tymovirales isolated from Prunus davidiana (Carr.) Franch. in Liaoning, China

Author:
Hou, Qiushi; Han, Tong; Li, Liang; Wang, Junzhu; Yu, Meichun; Zhang, Song; Cao, Mengji; Yang, Caixia
Source:
Archives of virology 2019 v.164 no.5 pp. 1245-1248
ISSN:
0304-8608
Subject:
Prunus davidiana; RNA; Tymovirales; amino acid sequences; coat proteins; enzymes; foliar diseases; genes; genetic relationships; high-throughput nucleotide sequencing; leaves; nucleotide sequences; nucleotides; phylogeny; sequence homology; virion; viruses; China; France
Abstract:
... In September 2017, a yellow spot leaf disease was noted on the leaves of Prunus davidiana (Carr.) Franch. plants in Liaoning, China, and spherical virions (approx. 30 nm in diameter) were later observed in preparations of symptomatic leaves. Subsequent deep sequencing of small RNA revealed the presence of a virus in these symptomatic leaves The complete genome of this viral isolate consists of 6,0 ...
DOI:
10.1007/s00705-019-04220-1