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1. Development of RapidHIT® ID using NGMSElect™ Express chemistry for the processing of reference samples within the UK criminal justice system

Author:
Shackleton, David; Gray, Nicola; Ives, Lesley; Malsom, Simon; Vanhinsbergh, Des
Source:
Forensic science international 2019 v.295 pp. 179-188
ISSN:
0379-0738
Subject:
DNA; chemistry; electrophoresis; forensic sciences; police; polymerase chain reaction; United Kingdom
Abstract:
... The RapidHIT® ID is a new instrument which has been designed specifically for non-technical users in non-laboratory environments, for example police custody suites or border control. The development studies presented here were performed using the AmpFℓSTR® NGMSElect™ Express STR kit with single source reference samples. The system has been successfully optimised for extraction, PCR and electrophor ...
DOI:
10.1016/j.forsciint.2018.12.015

2. DNA recovery from gelatin fingerprint lifters by direct proteolytic digestion

Author:
Zieger, Martin; Schneider, Christoph; Utz, Silvia
Source:
Forensic science international 2019 v.295 pp. 145-149
ISSN:
0379-0738
Subject:
DNA; DNA fingerprinting; forensic sciences; gelatin; magnetism; proteolysis; protocols; staining
Abstract:
... Fingerprints are a valuable source for DNA profiling in forensic investigations. In practice, the fingerprints are routinely visualized first by powder staining and then often transferred to tapes or gelatin lifters for storage or examination. If at all, fingerprints are usually sampled for DNA in a second step. To target the DNA sampling in an optimal way, it is essential to know how much of the ...
DOI:
10.1016/j.forsciint.2018.12.006

3. Human DNA degradation assessment and male DNA detection by quantitative-PCR followed by high-resolution melting analysis

Author:
Ginart, S.; Caputo, M.; Corach, D.; Sala, A.
Source:
Forensic science international 2019 v.295 pp. 1-7
ISSN:
0379-0738
Subject:
DNA; analytical kits; cost effectiveness; crime; females; humans; males; melting; quantitative polymerase chain reaction
Abstract:
... We developed a q-PCR technique that simultaneously evaluates the extent of degradation and determines the gender of a human DNA donor. QYDEG HRM is a triplex real-time PCR whose products are analysed by high-resolution melting (HRM). The system produces three amplicons: (1) transducin (beta)-like 1, Y-linked (TBL1Y) (84bp); (2) large-target sequence (DGlt) (244bp); and (3) small-target sequence (D ...
DOI:
10.1016/j.forsciint.2018.11.013

4. Prediction of DNA concentration in fingermarks using autofluorescence properties

Author:
Falkena, Kim; Hoveling, Richelle J.M.; van Weert, Angela; Lambrechts, Saskia A.G.; van Leeuwen, Ton G.; Aalders, Maurice C.G.; van Dam, Annemieke
Source:
Forensic science international 2019 v.295 pp. 128-136
ISSN:
0379-0738
Subject:
DNA; biocompatible materials; crime; forensic sciences; microsatellite repeats; prediction; quantitative polymerase chain reaction
Abstract:
... During criminal investigations trace DNA samples, including fingermarks, are submitted to laboratories for short tandem repeat (STR) analysis. For most common STR analysis systems a minimum amount of input DNA is required. Upon intake by the forensic laboratory the DNA concentration is estimated using quantitative polymerase chain reaction (qPCR) analysis after which most fingermarks are excluded. ...
DOI:
10.1016/j.forsciint.2018.12.005