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You searched for: Publication year rev 7981-2019 Remove constraint Publication year rev: 7981-2019 Subject biotin Remove constraint Subject: biotin Subject term CD8-positive T-lymphocytes Remove constraint Subject term: CD8-positive T-lymphocytes Subject term biotin Remove constraint Subject term: biotin
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1. Application of a novel FAM-conjugated activity-based probe to determine cathepsin G activity intracellularly

Author:
Roman Schroeder; Renata Grzywa; Christian Rainer Wirtz; Marcin Sienczyk; Timo Burster
Source:
Analytical biochemistry 2019 pp. -
ISSN:
0003-2697
Subject:
CD4-positive T-lymphocytes; CD8-positive T-lymphocytes; active sites; amino acid sequences; avidin; biotin; cathepsin G; chemical bonding; enzyme activity; flow cytometry; innate immunity; moieties; natural killer cells; phosphonates; serine; signal-to-noise ratio
Abstract:
... Cathepsin G (CatG) is responsible for several distinct immune processes of adaptive and innate immunity depending on extra- or intracellular occurrence of CatG. Recently, we established a method to detect CatG activity at the cell surface of natural killer cells by using the activity-based probe MARS116-Bt in flow cytometry. MARS116-Bt consists of biotin, spacer, amino acid sequence, and a phospho ...
DOI:
10.1016/j.ab.2019.113488

2. CD8 T cell-derived perforin aggravates secondary spinal cord injury through destroying the blood-spinal cord barrier

Author:
Zhaoxiang Liu; Hua Zhang; Hong Xia; Baocheng Wang; Renwen Zhang; Qun Zeng; Lingzhi Guo; Kui Shen; BaTa Wang; Yanheng Zhong; Zhizhong Li; Guodong Sun
Source:
Biochemical and biophysical research communications 2019 v.512 no.2 pp. 367-372
ISSN:
0006-291X
Subject:
CD8-positive T-lymphocytes; animal injuries; animal models; biotin; cerebrospinal fluid; cytokines; enzyme-linked immunosorbent assay; flow cytometry; humans; infectious diseases; inflammation; mice; permeability; spinal cord; staining
Abstract:
... Perforin plays an important role in autoimmune and infectious diseases, but its function in immune inflammatory responses after spinal cord injury (SCI) has received insufficient attention. The goal of this study is to determine the influence of perforin after spinal cord injury (SCI) on secondary inflammation. Compared recovery from SCI in perforin knockout (Prf1−/−) and wild-type(WT)mice, WT mic ...
DOI:
10.1016/j.bbrc.2019.03.002