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You searched for: Publication year rev 7981-2019 Remove constraint Publication year rev: 7981-2019 Subject term Enterobacteriaceae Remove constraint Subject term: Enterobacteriaceae Subject term active sites Remove constraint Subject term: active sites
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1. In vitro potentiation of carbapenems with tannic acid against carbapenemase‐producing enterobacteriaceae: exploring natural products as potential carbapenemase inhibitors

Author:
A.M. Somboro; J. Osei Sekyere; D.G. Amoako; H.M. Kumalo; R. Khan; L.A. Bester; S.Y. Essack
Source:
Journal of applied microbiology 2019 v.126 no.2 pp. 452-467
ISSN:
1364-5072
Subject:
Enterobacteriaceae; active sites; adjuvants; beta-lactamase; binding capacity; carbapenems; cytotoxicity; enzyme inhibition; enzyme inhibitors; hydrophobicity; inhibitory concentration 50; minimum inhibitory concentration; tannins; transporters
Abstract:
... AIMS: We hypothesized and confirmed that tannic acid (TA) reverses carbapenem resistance by inhibiting carbapenemases in class A and B carbapenemase‐producing Enterobacteriaceae. METHODS AND RESULTS: Minimum inhibitory concentrations of carbapenems in the presence and absence of TA and other efflux pump inhibitors, TA‐carbapenemases inhibition assays and computational studies showed that TA had th ...
DOI:
10.1111/jam.14141

2. Significant role of Asn-247 and Arg-64 residues in close proximity of the active site in maintaining the catalytic function of CTX-M-15 type β-lactamase

Author:
Lubna Maryam; Shamsi Khalid; Abid Ali; Asad U. Khan
Source:
RSC advances 2019 v.9 no.10 pp. 5325-5337
ISSN:
2046-2069
Subject:
Enterobacteriaceae; active sites; antibiotic resistance; arginine; asparagine; bacterial infections; beta-lactamase; cefotaxime; circular dichroism spectroscopy; fluorescence; hydrolysis; minimum inhibitory concentration; mutants; polymerase chain reaction; proteins; site-directed mutagenesis; spectral analysis
Abstract:
... Members of Enterobacteriaceae cause antibiotic-resistant infections worldwide. One such marker, CTX-M-15, expressed by Enterobacteriaceae produces β-lactamases, which hydrolyze the cephalosporin group of antibiotics, such as cefotaxime, used in the treatment of both Gram-positive and negative bacterial infections. Amino acid residues present in close proximity of the active site might also play a ...
DOI:
10.1039/c8ra10313e