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You searched for: Publication year rev 7981-2019 Remove constraint Publication year rev: 7981-2019 Subject term Escherichia coli Remove constraint Subject term: Escherichia coli Text availability Citation in PubAg Remove constraint Text availability: Citation in PubAg
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1. (p)ppGpp Regulates a Bacterial Nucleosidase by an Allosteric Two-Domain Switch

Author:
Yong Everett Zhang; René Lysdal Bærentsen; Tobias Fuhrer; Uwe Sauer; Kenn Gerdes; Ditlev Egeskov Brodersen
Source:
Molecular cell 2019 v.74 no.6 pp. 1239-1249.e4
ISSN:
1097-2765
Subject:
Escherichia coli; active sites; antibiotics; binding sites; biosynthesis; catalytic activity; enzymes; homeostasis; microbial physiology; models; nucleobases; nucleosides; stress response
Abstract:
... The stringent response alarmones pppGpp and ppGpp are essential for rapid adaption of bacterial physiology to changes in the environment. In Escherichia coli, the nucleosidase PpnN (YgdH) regulates purine homeostasis by cleaving nucleoside monophosphates and specifically binds (p)ppGpp. Here, we show that (p)ppGpp stimulates the catalytic activity of PpnN both in vitro and in vivo causing accumula ...
DOI:
10.1016/j.molcel.2019.03.035

2. (p)ppGpp: the magic governor of bacterial growth economy

Author:
Manlu Zhu; Yige Pan; Xiongfeng Dai
Source:
Current genetics 2019 v.65 no.5 pp. 1121-1125
ISSN:
0172-8083
Subject:
Escherichia coli; bacterial growth; cell growth; gene expression; proteomics; resource allocation; ribosomes; second messengers; stress response; transcriptomics
Abstract:
... A fundamental question in microbiology is how bacterial cells manage to coordinate gene expression with cell growth during adapting to various environmental conditions. Although the cellular responses to changing environments have been extensively studied using transcriptomic and proteomic approaches, it remains poorly understood regarding the molecular strategy enabling bacteria to manipulate the ...
DOI:
10.1007/s00294-019-00973-z

3. 20-Hydroxyecdysone regulates the transcription of the lysozyme via Broad-Complex Z2 gene in silkworm, Bombyx mori

Author:
Hanxiu Ma; Muhammad Nadeem Abbas; Kui Zhang; Xiaosong Hu; Man Xu; Hanghua Liang; Saima Kausar; Liqun Yang; Hongjuan Cui
Source:
Developmental and comparative immunology 2019 v.94 pp. 66-72
ISSN:
0145-305X
Subject:
Bombyx mori; Escherichia coli; antimicrobial peptides; ecdysone; ecdysterone; gene expression regulation; genes; hemocytes; immune response; innate immunity; insect larvae; lysozyme; messenger RNA; molting; pathogen-associated molecular patterns; pathogens; promoter regions; quantitative polymerase chain reaction; silkworms; survival rate; tissues; transcription (genetics); transcription factors
Abstract:
... Broad-Complex Z2 (Br-C Z2) is an ecdysone inducible transcription factor that regulates physiological, innate immune and developmental events in insects. Here, we identified an orthologue of Br-C Z2 from silkworm, Bombyx mori (BmBr-C Z2) to study its involvement in immune responses. The quantitative real-time PCR analysis revealed that BmBr-C Z2 was expressed ubiquitously in all tested tissues und ...
DOI:
10.1016/j.dci.2019.01.014

4. 3-Phosphoglycerate Transhydrogenation Instead of Dehydrogenation Alleviates the Redox State Dependency of Yeast de Novol-Serine Synthesis

Author:
Nicole Paczia; Julia Becker-Kettern; Jean-François Conrotte; Javier O. Cifuente; Marcelo E. Guerin; Carole L. Linster
Source:
Biochemistry 2019 v.58 no.4 pp. 259-275
ISSN:
1520-4995
Subject:
Escherichia coli; NAD (coenzyme); Pseudomonas; Saccharomyces cerevisiae; alpha-ketoglutaric acid; carbon metabolism; dehydrogenation; enzyme activity; enzymes; genes; humans; in vitro studies; oxidation; phenotype; reaction mechanisms; serine; yeasts
Abstract:
... The enzymatic mechanism of 3-phosphoglycerate to 3-phosphohydroxypyruvate oxidation, which forms the first step of the main conserved de novo serine synthesis pathway, has been revisited recently in certain microorganisms. While this step is classically considered to be catalyzed by an NAD-dependent dehydrogenase (e.g., PHGDH in mammals), evidence has shown that in Pseudomonas, Escherichia coli, a ...
DOI:
10.1021/acs.biochem.8b00990

5. A CUGGU/UUGGU-specific MazF homologue from Methanohalobium evestigatum

Author:
Yojiro Ishida; Keiko Inouye; Ouyang Ming; Masayori Inouye
Source:
Biochemical and biophysical research communications 2019 v.518 no.3 pp. 533-540
ISSN:
0006-291X
Subject:
Escherichia coli; antitoxins; genes; research; ribonucleotides; toxicity
Abstract:
... MazF is a sequence-specific endoribonuclease or mRNA interferase, which cleaves RNA at a specific sequence. Since the expression of a specific gene or a group of specific genes can be regulated by MazF, expanding the repertoire of recognition sequences by MazF mRNA interferases is highly desirable for biotechnological and medical applications. Here, we identified a gene for a MazF homologue (MazFm ...
DOI:
10.1016/j.bbrc.2019.08.076

6. A Conceptual Framework for Developing Recommendations for No-Harvest Buffers around In-Field Feces

Author:
Daniel L. Weller; Jasna Kovac; David J. Kent; Sherry Roof; Jeffrey I. Tokman; Erika Mudrak; Martin Wiedmann
Source:
Journal of food protection 2019 v.82 no.6 pp. 1052-1060
ISSN:
0362-028X
Subject:
Escherichia coli; acceptable risk; buffers; confidence interval; feces; food pathogens; harvesting; irrigation; lettuce; microorganisms; odds ratio; pellets; regression analysis; wildlife
Abstract:
... Results of previous studies revealed that (i) splash can transfer microbes from in-field feces to preharvest produce and (ii) wildlife can be vectors for the introduction of foodborne pathogens into produce fields. However, few peer-reviewed studies have been conducted to examine pathogen transfer from wildlife feces to in-field produce via splash during irrigation. Although two previous studies f ...
DOI:
10.4315/0362-028X.JFP-18-414

7. A C‐type lectin with a single carbohydrate‐recognition domain involved in the innate immune response of Tribolium castaneum

Author:
J. Bi; F. Feng; J. Li; J. Mao; M. Ning; X. Song; J. Xie; J. Tang; B. Li
Source:
Insect molecular biology 2019 v.28 no.5 pp. 649-661
ISSN:
0962-1075
Subject:
Bacillus subtilis; Bacillus thuringiensis; Escherichia coli; Gram-negative bacteria; Pseudomonas aeruginosa; RNA interference; Staphylococcus aureus; Tribolium castaneum; agglutination; amino acids; antimicrobial peptides; cDNA libraries; calcium; clones; complementary DNA; genes; innate immunity; invertebrates; ions; lectins; lipopolysaccharides; open reading frames; pathogens; peptidoglycans
Abstract:
... C‐type lectins are one of the pattern‐recognition proteins involved in innate immunity in invertebrates. Although there are 16 C‐type lectin genes that have been identified in the genome of Tribolium castaneum, their functions and mechanisms in innate immunity remain unknown. Here, we identified one C‐type lectin orthologue, TcCTL6 (TC003708), by sequencing random clones from the cDNA library of t ...
DOI:
10.1111/imb.12582

8. A High-Throughput Platform for the Generation of Synthetic Ab Clones by Single-Strand Site-Directed Mutagenesis

Author:
Eugenio Gallo
Source:
Molecular biotechnology 2019 v.61 no.6 pp. 410-420
ISSN:
1073-6085
Subject:
Escherichia coli; antibodies; antigens; clones; cost effectiveness; metadata; models; molecular cloning; protein synthesis; proteins; rapid methods; restriction endonucleases; screening; sequence analysis; site-directed mutagenesis; stop codon
Abstract:
... Current developments in meta-data analysis and predictive computational models offer alternative routes for the identification of antibodies. In silico-based technologies and NGS data analysis from Ab phage-display selections offer expanded selections of Ab candidates. Accordingly, the identified de novo Abs with predicted selectivity for a target antigen must undergo rapid gene synthesis for down ...
DOI:
10.1007/s12033-019-00171-9

9. A JAK-STAT pathway target gene encoding a single WAP domain (SWD)-containing protein from Litopenaeus vannamei

Author:
Linwei Yang; Mengting Luo; Jianhui He; Hongliang Zuo; Shaoping Weng; Jianguo He; Xiaopeng Xu
Source:
Fish & shellfish immunology 2019 v.89 pp. 555-563
ISSN:
1050-4648
Subject:
Bacillus subtilis; Escherichia coli; Gram-negative bacteria; Gram-positive bacteria; Litopenaeus vannamei; Staphylococcus aureus; Vibrio parahaemolyticus; fungi; gene expression regulation; gene overexpression; genes; hemocytes; immune response; in vitro studies; nucleotide motifs; peptides; phagocytosis; shrimp; transcription (genetics); viruses
Abstract:
... In shrimp, the JAK-STAT pathway is essentially implicated in both antiviral and antibacterial responses. However, few regulatory target genes of the JAK-STAT pathway in shrimp have been reported so far. In this study, a novel single WAP domain-containing peptide (LvSWD4) was identified from Pacific white shrimp Litopenaeus vannamei. The promoter of LvSWD4 was predicted to harbor multiple STAT-bind ...
DOI:
10.1016/j.fsi.2019.04.046

10. A Membrane-Bound Gluconate Dehydrogenase from 2-Keto-D-Gluconic Acid Industrial Producing Strain Pseudomonas plecoglossicida JUIM01: Purification, Characterization, and Gene Identification

Author:
Da-Ming Wang; Lei Sun; Wen-Jing Sun; Feng-Jie Cui; Jin-Song Gong; Xiao-Mei Zhang; Jin-Song Shi; Zheng-Hong Xu
Source:
Applied biochemistry and biotechnology 2019 v.188 no.4 pp. 897-913
ISSN:
0273-2289
Subject:
Escherichia coli; Pseudomonas plecoglossicida; calcium; cytochrome c; enzyme activity; enzymes; flavoproteins; genetic engineering; gluconates; magnesium; manganese; metal ions; molecular weight; multigene family; pH; recombinant proteins; temperature
Abstract:
... The membrane-bound gluconate dehydrogenase (mGADH) is a critical enzyme for 2-keto-D-gluconic acid (2KGA) production in Pseudomonas plecoglossicida JUIM01. The purified native flavin adenine dinucleotide-dependent mGADH (FAD-mGADH) was consisted of a gamma subunit, a flavoprotein subunit, and a cytochrome c subunit with molecular mass of ~ 27, 65, and 47 kDa, respectively. The specific activity of ...
DOI:
10.1007/s12010-019-02951-0

11. A Multiplex Fragment-Ion-Based Method for Accurate Proteome Quantification

Author:
Jianhui Liu; Yuan Zhou; Yichu Shan; Baofeng Zhao; Yechen Hu; Zhigang Sui; Zhen Liang; Lihua Zhang; Yukui Zhang
Source:
Analytical chemistry 2019 v.91 no.6 pp. 3921-3928
ISSN:
1520-6882
Subject:
Escherichia coli; adenocarcinoma; cell lines; data collection; enzymes; ions; lungs; models; post-translational modification; proteome; proteomics; regulatory proteins
Abstract:
... Multiplex proteome quantification with high accuracy is urgently required to achieve a comprehensive understanding of dynamic cellular and physiological processes. Among the existing quantification strategies, fragment-ion-based methods can provide highly accurate results, but the multiplex capacity is limited to 3-plex. Herein, we developed a multiplex pseudo-isobaric dimethyl labeling (m-pIDL) m ...
DOI:
10.1021/acs.analchem.8b04806

12. A Randomized, Double-Blind, Placebo-Controlled Pilot Study to Assess the Urinary Anti-Adhesion Activity Following Consumption of Cranberry + health™ Cranberry Supplement (P06-114-19)

Author:
Haiyan Liu; Christina Khoo
Source:
Current developments in nutrition 2019 v.3 no.Supplement_1 pp. -
ISSN:
2475-2991
Subject:
Escherichia coli; Vaccinium macrocarpon; adhesion; antibiotics; bacterial infections; bladder; cranberries; disease control; erythrocytes; fimbriae; hemagglutination; human cell lines; metabolites; placebos; urinary tract diseases; urine; women
Abstract:
... Urinary tract infections (UTIs) are the second most common bacterial infections and are often treated with antibiotics. The North American cranberry is recommended for UTI prophylaxis in women with recurrent UTI as a nutritional alternative approach. The ability of cranberry components or their metabolites to inhibit the adhesion of uropathogenic E.coli to the bladder is considered as an important ...
DOI:
10.1093/cdn/nzz031.P06-114-19

13. A Randomized, Double-blind, Controlled Clinical Trial to Evaluate the Safety and Immunogenicity of an Intranasally Administered Trivalent Inactivated Influenza Vaccine with Adjuvant LTh(αK): A Phase I Study

Author:
Sung-Ching Pan; Szu-Min Hsieh; Chih-Feng Lin; Yu-Shen Hsu; Mingi Chang; Shan-Chwen Chang
Source:
Vaccine 2019 pp. -
ISSN:
0264-410X
Subject:
Escherichia coli; Influenza A virus; adjuvants; antibodies; antigens; arrhythmia; clinical trials; enterotoxins; fever; headache; hemagglutination; immunogenicity; immunoglobulin A; influenza; influenza vaccines; live vaccines; mucosal immunity; nose; viruses
Abstract:
... A nasal influenza vaccine has been available only in a live attenuated form, which limits the range of recipients to immune-competent individuals. The present study evaluated a newly developed intranasal inactivated influenza vaccine with a novel adjuvant, heat-labile enterotoxin (LT) derived from E. coli (LTh(αK)).The study was a randomized, double-blind, controlled phase I trial to evaluate the ...
DOI:
10.1016/j.vaccine.2019.02.006

14. A bactericidal permeability-increasing protein (BPI) from manila clam Ruditapes philippinarum: Investigation on the antibacterial activities and antibacterial action mode

Author:
Dinglong Yang; Yijing Han; Lizhu Chen; Ruiwen Cao; Qing Wang; Zhijun Dong; Hui Liu; Xiaoli Zhang; Qianqian Zhang; Jianmin Zhao
Source:
Fish & shellfish immunology 2019 v.93 pp. 841-850
ISSN:
1050-4648
Subject:
Escherichia coli; Gram-negative bacteria; Ruditapes philippinarum; Staphylococcus aureus; Vibrio anguillarum; antibacterial properties; antimicrobial proteins; bacterial infections; cell death; clams; gene expression regulation; hemocytes; innate immunity; membrane permeability; messenger RNA; phylogeny; shellfish
Abstract:
... Bactericidal permeability-increasing protein (BPI) is an antimicrobial protein with potent endotoxin-neutralising activity and plays a crucial role in innate immunity against bacterial infection. In the present study, a bpi (designed as rpbpi) was identified and characterized from manila clam Ruditapes philippinarum. Multiple alignments and phylogenetic analysis suggested that rpbpi was a new memb ...
DOI:
10.1016/j.fsi.2019.08.050

15. A bio-inspired strategy for the synthesis of zinc oxide nanoparticles (ZnO NPs) using the cell extract of cyanobacterium Nostoc sp. EA03: from biological function to toxicity evaluation

Author:
Mojgan Ebadi; Mohammad Reza Zolfaghari; Seyyed Soheil Aghaei; Mohsen Zargar; Morvarid Shafiei; Hossein Shahbani Zahiri; Kambiz Akbari Noghabi
Source:
RSC advances 2019 v.9 no.41 pp. 23508-23525
ISSN:
2046-2069
Subject:
Escherichia coli; Fourier transform infrared spectroscopy; Nostoc; Pseudomonas aeruginosa; Staphylococcus aureus; X-ray diffraction; absorption; amino acid derivatives; bacterial growth; bioactive compounds; biofilm; confocal laser scanning microscopy; cytotoxicity; differential scanning calorimetry; energy-dispersive X-ray analysis; exopolysaccharides; fibroblasts; flow cytometry; lungs; nanoparticles; photosynthetic bacteria; pigments; proteins; scanning electron microscopy; thermogravimetry; toxicity testing; transmission electron microscopy; ultraviolet-visible spectroscopy; viability; zeta potential; zinc oxide
Abstract:
... Cyanobacteria, as one of the largest groups of phototrophic bacteria, have a high potential as an excellent source of fine chemicals and bioactive compounds, including lipid-like compounds, amino acid derivatives, proteins, and pigments. This study aimed to synthesize ZnO nanoparticles using the cell extract of the cyanobacterium Nostoc sp. EA03 (CEN-ZnO NPs) through a rapid and eco-friendly appro ...
DOI:
10.1039/c9ra03962g

16. A bootstrap-aggregated hybrid semi-parametric modeling framework for bioprocess development

Author:
José Pinto; Cristiana Rodrigues de Azevedo; Rui Oliveira; Moritz von Stosch
Source:
Bioprocess and biosystems engineering 2019 v.42 no.11 pp. 1853-1865
ISSN:
1615-7591
Subject:
Escherichia coli; artificial intelligence; biomass; bioprocessing; cell growth; data collection; experimental design; models; prediction; protein synthesis; recombinant proteins; system optimization; temperature
Abstract:
... Hybrid semi-parametric modeling, combining mechanistic and machine-learning methods, has proven to be a powerful method for process development. This paper proposes bootstrap aggregation to increase the predictive power of hybrid semi-parametric models when the process data are obtained by statistical design of experiments. A fed-batch Escherichia coli optimization problem is addressed, in which t ...
DOI:
10.1007/s00449-019-02181-y

17. A closer look in the antimicrobial properties of deep eutectic solvents based on fatty acids

Author:
Joana M. Silva; Eduardo Silva; Rui L. Reis; Ana Rita C. Duarte
Source:
Sustainable chemistry and pharmacy 2019 v.14 pp. 100192
ISSN:
2352-5541
Subject:
Escherichia coli; Gram-positive bacteria; antibacterial properties; biofilm; decanoic acid; dodecanoic acid; green chemistry; medical equipment; melting point; microscopy; myristic acid; physicochemical properties; solvents; stearic acid; synergism; temperature; therapeutics; thermal properties; viscosity
Abstract:
... Microbial infection is a serious and challenging clinical complication that has attracted widespread interest in last decades. In this context, we present a strategy based on deep eutectic solvents (DES) to explore these unmet medical needs. DES systems based on saturated fatty acids, namely, capric acid (CA), myristic acid (MA), lauric acid (LA) and stearic acid (SA) were produced and fully chara ...
DOI:
10.1016/j.scp.2019.100192

18. A comparative study on the essential oil composition and antibacterial activities of different organs of wild growing Paeonia daurica subsp. tomentosa from Iran

Author:
Edris Mahdavi Fikejvar; Hassan Rezadoost; Hedayat Zakizadeh; Valiollah Mozaffarian
Source:
Natural product research 2019 v.33 no.21 pp. 3153-3156
ISSN:
1478-6427
Subject:
Escherichia coli; Paeonia; Staphylococcus aureus; alpha-pinene; antibacterial properties; comparative study; essential oils; fruits; gas chromatography-mass spectrometry; hydroxybenzaldehyde; leaves; oils; roots; stems; Iran
Abstract:
... Paeonia daurica subsp. tomentosa (Lomakin) D.Y.Hong is an herbaceous perennial and flowering plant from the Paeoniaceae family. In this research, the roots, stems, leaves and fruits of P. daurica subsp. tomentosa were investigated for a comparative identification of essential oil composition. The essential oils constituents were identified by GC/MS and then compared. The study leads to the identif ...
DOI:
10.1080/14786419.2018.1516665

19. A concerted systems biology analysis of phenol metabolism in Rhodococcus opacus PD630

Author:
Garrett W. Roell; Rhiannon R. Carr; Tayte Campbell; Zeyu Shang; William R. Henson; Jeffrey J. Czajka; Hector García Martín; Fuzhong Zhang; Marcus Foston; Gautam Dantas; Tae Seok Moon; Yinjie J. Tang
Source:
Metabolic engineering 2019 v.55 pp. 120-130
ISSN:
1096-7176
Subject:
Escherichia coli; NADP (coenzyme); Rhodococcus opacus; acetates; alpha-ketoglutaric acid; biomass; carbon; catabolite repression; fructose-bisphosphatase; gluconeogenesis; glucose; hydrolysates; isocitrate dehydrogenase; lignin; lignocellulose; lipids; malates; metabolites; phenol; phosphates; secretion; transcription (genetics); tricarboxylic acid cycle
Abstract:
... Rhodococcus opacus PD630 metabolizes aromatic substrates and naturally produces branched-chain lipids, which are advantageous traits for lignin valorization. To provide insights into its lignocellulose hydrolysate utilization, we performed 13C-pathway tracing, 13C-pulse-tracing, transcriptional profiling, biomass composition analysis, and metabolite profiling in conjunction with 13C-metabolic flux ...
DOI:
10.1016/j.ymben.2019.06.013

20. A conformational switch from a closed apo- to an open holo-form equips the acyl carrier protein for acyl chain accommodation

Author:
Richa Arya; Bhaskar Sharma; Chetna Dhembla; Ravi Kant Pal; Ashok Kumar Patel; Monica Sundd; Biplab Ghosh; Ravindra D. Makde; Suman Kundu
Source:
Biochimica et biophysica acta 2019 v.1867 no.3 pp. 163-174
ISSN:
1570-9639
Subject:
Escherichia coli; Leishmania major; Plasmodium falciparum; acyl carrier protein; biosynthesis; crystal structure; dissociation; enzymes; fatty acids; mutants; nuclear magnetic resonance spectroscopy; polyketides; polypeptides
Abstract:
... Acyl carrier proteins (ACPs) play crucial roles in the biosynthesis of fatty acids, non-ribosomal polypeptides and polyketides. The three-dimensional NMR structure of Leishmania major holo-LmACP, belonging to the type II pathway, has been reported previously, but the structure of its apo-form and its conformational differences with the holo-form remain to be explored. Here we report the crystal st ...
DOI:
10.1016/j.bbapap.2018.12.001

21. A critical challenge in the development of antibody: Selecting the appropriate fragment of the target protein as an antigen based on various epitopes or similar structure

Author:
Mahboubeh Razzaqi; Mohammad Javad Rasaee; Maliheh Paknejad
Source:
Molecular immunology 2019 v.111 pp. 128-135
ISSN:
0161-5890
Subject:
Escherichia coli; affinity chromatography; analysis of variance; circular dichroism spectroscopy; epitopes; immune response; immunization; mice; monoclonal antibodies; neoplasms; prediction; proteins; pyruvate kinase; vaccines
Abstract:
... The main challenge in the development of antibody is to select the appropriate antigen particularly when a truncated protein is used for immunization or as vaccine antigen. In previous studies, fragment selection was mainly based on epitopes and less often on the structure. Fewer studies have paid attention to the prediction of the truncated protein 3D structure and retained its similarity in the ...
DOI:
10.1016/j.molimm.2019.04.018

22. A duplex PCR assay for the simultaneous detection and differentiation of Muscovy duck parvovirus and goose parvovirus

Author:
Chunhe Wan; Longfei Cheng; Cuiteng Chen; Rongchang Liu; Shaohua Shi; Guanghua Fu; Hongmei Chen; Qiuling Fu; Yu Huang
Source:
Molecular and cellular probes 2019 pp. -
ISSN:
0890-8508
Subject:
Anseriform dependoparvovirus 1; Cairina moschata; Escherichia coli; Muscovy; detection limit; ducklings; genes; morbidity; mortality; nucleic acids; pathogens; polymerase chain reaction; vaccination; vaccines; waterfowl
Abstract:
... Both Muscovy duck parvovirus (MDPV) and goose parvovirus (GPV) can cause high mortality and morbidity in Muscovy ducklings. MDPVs and GPVs share high nucleotide identity, which can cause errors during differential diagnosis. In this study, the NS genes of both MDPVs and GPVs were chosen for the design of specific primers after genetic comparison. Only three primers (GF1, MF1 and MGR1) were designe ...
DOI:
10.1016/j.mcp.2019.101439

23. A facile and robust non-natural three enzyme biocatalytic cascade based on Escherichia coli surface assembly for fatty alcohol production

Author:
Yun Xu; Fei Li; Kaixin Yang; Yangge Qiao; Yunjun Yan; Jinyong Yan
Source:
Energy conversion and management 2019 v.181 pp. 501-506
ISSN:
0196-8904
Subject:
Escherichia coli; aldehyde reductase; biosynthesis; biotransformation; carboxylic acids; fatty alcohols; fossil fuels; solvents; temperature; triacylglycerol lipase; triacylglycerols
Abstract:
... Fatty alcohol is considered as a promising clean fuel alternative to fossil fuel. In this study, we developed a facile and robust multiple enzyme complex of lipase (LIP), carboxylic acid reductase (CAR) and aldehyde reductase (AHR) based on cohesin-dockerin specific interaction and E. coli-ice nucleation protein (INP) surface assembly for highly efficient conversion of triacylglycerols (TAGs) to f ...
DOI:
10.1016/j.enconman.2018.12.042

24. A heteromeric cis-prenyltransferase is responsible for the biosynthesis of glycosyl carrier lipids in Methanosarcina mazei

Author:
Koh-ichi Emi; Kitty Sompiyachoke; Miyako Okada; Hisashi Hemmi
Source:
Biochemical and biophysical research communications 2019 v.520 no.2 pp. 291-296
ISSN:
0006-291X
Subject:
Escherichia coli; Methanosarcina mazei; biosynthesis; dimethylallyltranstransferase; lipids; methanogens; research; rubber; secondary metabolites
Abstract:
... Cis-prenyltransferases are enzymes responsible for the biosynthesis of glycosyl carrier lipids, natural rubber, and some secondary metabolites. Certain organisms, including some archaeal species, possess multiple genes encoding cis-prenyltransferase homologs, and the physiological roles of these seemingly-redundant genes are often obscure. Cis-prenyltransferases usually form homomeric complexes, b ...
DOI:
10.1016/j.bbrc.2019.09.143

25. A highly active α-cyclodextrin preferring cyclomaltodextrinase from Geobacillus thermopakistaniensis

Author:
Iqra Aroob; Nasir Ahmad; Mehwish Aslam; Abeera Shaeer; Naeem Rashid
Source:
Carbohydrate research 2019 v.481 pp. 1-8
ISSN:
0008-6215
Subject:
EDTA (chelating agent); Escherichia coli; Geobacillus; acarbose; alpha-cyclodextrin; amide hydrolases; enzyme activity; gamma-cyclodextrin; gene overexpression; genes; glycosidases; glycosylation; hydrolysis; maltotriose; manganese; metal ions; pH; pullulan; starch; thermal stability
Abstract:
... Cyclomaltodextrinases show diverse hydrolyzing and/or transglycosylation activities against cyclodextrins, starch and pullulan. A gene annotated as cyclomaltodextrinase from Geobacillus thermopakistaniensis was cloned and overexpressed in Escherichia coli. The gene product, CDaseGt, was purified and biochemically characterized. The recombinant enzyme exhibited highest activity with α-cyclodextrin ...
DOI:
10.1016/j.carres.2019.06.004

26. A laboratory‐based evaluation of tube blocking and microbial risks associated with one blended enteral feed recipe

Author:
A. M. Madden; S. Baines; S. Bothwell; E. Chen; S. Goh; L. Jerome; C. Sommariva‐Nagle; M. Szychta
Source:
Journal of human nutrition and dietetics 2019 v.32 no.5 pp. 667-675
ISSN:
0952-3871
Subject:
Adequate Intakes; Clostridium; Enterococcus; Escherichia coli; Pseudomonas; Salmonella; Shigella; Staphylococcus epidermidis; Streptococcus lutetiensis; Vibrio; bacteria; bacterial contamination; blenders; enteral feeding; feeds; frozen storage; nutritional adequacy; pathogens; patients; phenotype; polymerase chain reaction; ribosomal RNA; risk; storage temperature
Abstract:
... BACKGROUND: Concerns associated with blended enteral feeds include the risk of blocked tubes and microbial contamination, although the available evidence is limited. The present laboratory‐based investigation aimed to examine these risks in a blended feed providing a nutritionally adequate intake for a hypothetical patient. METHODS: A one‐blended feed recipe was made using three different methods ...
DOI:
10.1111/jhn.12685

27. A macin identified from Venerupis philippinarum: Investigation on antibacterial activities and action mode

Author:
Dinglong Yang; Yijing Han; Lizhu Chen; Ruiwen Cao; Qing Wang; Zhijun Dong; Hui Liu; Xiaoli Zhang; Qianqian Zhang; Jianmin Zhao
Source:
Fish & shellfish immunology 2019 v.92 pp. 897-904
ISSN:
1050-4648
Subject:
Escherichia coli; Gram-negative bacteria; Gram-positive bacteria; Ruditapes philippinarum; Vibrio anguillarum; amino acids; antibacterial properties; cell death; clams; complementary DNA; gills; hemocytes; hepatopancreas; membrane permeability; molecular cloning; molecular weight; pathogens; peptides; shellfish
Abstract:
... In the present study, a macin was cloned and characterized from clam Venerupis philippinarum (designed as VpMacin). The full-length cDNA of VpMacin was of 579 bp, encoding a peptide of 87 amino acids with the predicted molecular weight of 9.7 kDa. Analysis of the conserved domain suggested that VpMacin was a new member of the macin family. In non-stimulated clams, VpMacin transcripts exhibited dif ...
DOI:
10.1016/j.fsi.2019.07.031

28. A multiplexed, indirect enzyme-linked immunoassay for the detection and differentiation of E. coli from other Enterobacteriaceae and P. aeruginosa from other glucose non-fermenters

Author:
N. Jackson; T.Z. Wu; S. Adams-Sapper; T. Satoorian; M. Geisberg; N. Murthy; L. Lee; L.W. Riley
Source:
Journal of microbiological methods 2019 v.158 pp. 52-58
ISSN:
0167-7012
Subject:
Escherichia coli; Gram-negative bacteria; Pseudomonas aeruginosa; antibodies; diagnostic techniques; enzyme-linked immunosorbent assay; glucose; hospitals; pathogens
Abstract:
... Gram-negative bacteria (GNB) are important causes of community (CA) and hospital (HA)- associated infections. Here we describe the development of an indirect ELISA (I-ELISA), which can be used to detect and differentiate the Enterobacteriaceae Escherichia coli, and glucose non-fermenter Pseudomonas aeruginosa from other GNB species. The I-ELISA utilizes six antibodies for bacterial speciation, whi ...
DOI:
10.1016/j.mimet.2019.01.014

29. A multivariate statistical approach to the integration of different land-uses, seasons, and water quality as water resources management tool

Author:
Mariana Antonio de Souza Pereira; Priscila Sabioni Cavalheri; Michel Ângelo Constantino de Oliveira; Fernando Jorge Correa Magalhães Filho
Source:
Environmental monitoring and assessment 2019 v.191 no.9 pp. 539
ISSN:
0167-6369
Subject:
Escherichia coli; cluster analysis; coliform bacteria; data collection; decision making; dissolved oxygen; dry season; land use; nitrogen; nutrients; oxygen; phosphorus; planning; population density; principal component analysis; rural areas; sanitation; spring; surface water; temperature; topography; total nitrogen; total phosphorus; urban areas; urbanization; variance; water management; water pollution; water quality; winter
Abstract:
... The externalities generated by disorderly urbanization and lack of proper planning becomes one of the main factors that must be considered in water resource management. To address the multiple uses of water and avoid conflicts among users, decision-making must integrate these factors into quality and quantity aspects. The water quality index (WQI), using the correlation matrix and the multivariate ...
DOI:
10.1007/s10661-019-7647-1

30. A new active nanocomposite film based on PLA/ZnO nanoparticle/essential oils for the preservation of refrigerated Otolithes ruber fillets

Author:
Mojtaba Heydari-Majd; Babak Ghanbarzadeh; Mostafa Shahidi-Noghabi; Mohammad Ali Najafi; Mohammadyar Hosseini
Source:
Food packaging and shelf life 2019 v.19 pp. 94-103
ISSN:
2214-2894
Subject:
Bacillus cereus; Escherichia coli; Otolithes ruber; Pseudomonas aeruginosa; Salmonella enterica; Staphylococcus aureus; Zataria multiflora; active food packaging; antibacterial properties; antioxidants; atomic absorption spectrometry; carvacrol; cold storage; essential oils; fillets; fish; food pathogens; ions; lactic acid bacteria; meat; menthone; muscles; nanocomposites; nanoparticles; packaging films; refrigeration; shelf life; thiobarbituric acid-reactive substances; total volatile basic nitrogen; zinc; zinc oxide
Abstract:
... In the current study, the active packaging films based on poly lactic acid (PLA) containing 1.5% w/w zinc oxide (ZnO) nanoparticles and varying concentrations (0.5, 1, 1.5% w/w) of Zataria multiflora Boiss. essential oil (ZEO) and Menthe piperita L. essential oil (MEO) were produced. The efficacies of the resulted active films on shelf life extension of Otolithes ruber fish during 16 days of refri ...
DOI:
10.1016/j.fpsl.2018.12.002

31. A new crustin homologue (SpCrus6) involved in the antimicrobial and antiviral innate immunity in mud crab, Scylla paramamosain

Author:
Zhi-qiang Du; Yue Wang; Hong-yu Ma; Xiu-li Shen; Kai Wang; Jie Du; Xiao-dong Yu; Wen-hong Fang; Xin-cang Li
Source:
Fish & shellfish immunology 2019 v.84 pp. 733-743
ISSN:
1050-4648
Subject:
Bacillus megaterium; Bacillus subtilis; Candida albicans; Escherichia coli; Gram-negative bacteria; Gram-positive bacteria; Komagataella pastoris; Scylla paramamosain; Staphylococcus aureus; Vibrio alginolyticus; Vibrio harveyi; Vibrio parahaemolyticus; White spot syndrome virus; beta-glucans; crabs; crustin; cysteine; fish; fungi; gene expression regulation; genes; innate immunity; messenger RNA; open reading frames; reproduction; shellfish; signal peptide; viruses
Abstract:
... Crustins play important roles in defending against bacteria in the innate immunity system of crustaceans. In present study, we identified a crustin gene in Scylla paramamosain, which was named as SpCrus6. The ORF of SpCrus6 possessed a signal peptide sequence (SPS) at the N-terminus and a WAP domain at the C-terminus. And there were 5 Proline residues, 5 Glycine and 4 Cysteine residues between SPS ...
DOI:
10.1016/j.fsi.2018.10.072

32. A new method to treat farm dairy effluent to produce clarified water for recycling and to reduce environmental risks from the land application of effluent

Author:
Keith C. Cameron; Hong J. Di
Source:
Journal of soils and sediments 2019 v.19 no.5 pp. 2290-2302
ISSN:
1439-0108
Subject:
Escherichia coli; coagulants; colloids; dairy farming; environmental impact; farms; field experimentation; freshwater; land application; pastures; plant growth; reactive phosphorus; recycling; research and development; risk; total nitrogen; turbidity; washing; New Zealand
Abstract:
... PURPOSE: There is increasing public concern about the amount of fresh water used on New Zealand dairy farms and the adverse impacts that farm dairy effluent (FDE) has on the environment. FDE mainly consists of fresh water (99%) with a small amount of solid material (1%). There is an opportunity to treat FDE to remove the colloidal solid material and recycle the water to wash the farm yard. MATERIA ...
DOI:
10.1007/s11368-018-02227-w

33. A new mucin-binding lectin from the marine sponge Aplysina fulva (AFL) exhibits antibiofilm effects

Author:
Rômulo Farias Carneiro; Jhonatas Teixeira Viana; Renato Cézar Farias Torres; Lídia Torquato da Silva; Alexandre Lopes Andrade; Mayron Alves de Vasconcelos; Ulisses Pinheiro; Edson Holanda Teixeira; Celso Shiniti Nagano; Alexandre Holanda Sampaio
Source:
Archives of biochemistry and biophysics 2019 v.662 pp. 169-176
ISSN:
0003-9861
Subject:
Escherichia coli; Gram-negative bacteria; Porifera; Staphylococcus aureus; Staphylococcus epidermidis; affinity chromatography; amino acids; biofilm; biomass; disulfide bonds; hemagglutination; ions; lectins; matrix-assisted laser desorption-ionization mass spectrometry; mucins; pH; peptides; plankton; polyacrylamide gel electrophoresis; sequence homology; stomach; sugars; swine; temperature
Abstract:
... A new mucin-binding lectin (AFL) was isolated from the marine sponge Aplysina fulva. AFL was purified by affinity chromatography on Sepharose™ matrix. Its hemagglutinating activity was independent of divalent ions, and it was weakly inhibited by simple sugars. However, porcine stomach mucin was a powerful inhibitor. In SDS PAGE, piridylethylated AFL showed one band of approximately 16 kDa, whereas ...
DOI:
10.1016/j.abb.2018.12.014

34. A new route for the preparation of hydrophobic and antibacterial textiles fabrics using Ag-loaded graphene nanocomposite

Author:
Boubker Ouadil; Othmane Amadine; Younes Essamlali; Omar Cherkaoui; Mohamed Zahouily
Source:
Colloids and surfaces 2019 v.579 pp. 123713
ISSN:
0927-7757
Subject:
Escherichia coli; Raman spectroscopy; Staphylococcus aureus; antibacterial properties; bacteria; coatings; color; contact angle; cost effectiveness; dimensional stability; drying; fabrics; graphene; hydrophobicity; light microscopy; nanocomposites; nanosheets; nanosilver; nitrogen; polyethylene terephthalates; scanning electron microscopy; stabilizers; strength (mechanics); surface area; synergism; textile industry; thermal stability; wettability
Abstract:
... Polyethylene terephthalate (PET) has been widely used as a basic material in fabrics for a wide range of applications owing to its excellent physicochemical and mechanical properties such as excellent mechanical strength, easy treatment, quick drying and dimensional stability. However, conventional PET-based fabrics always suffer from several issues mainly related to bad surface wettability and po ...
DOI:
10.1016/j.colsurfa.2019.123713

35. A non-secreted plant defensin AtPDF2.6 conferred cadmium tolerance via its chelation in Arabidopsis

Author:
Jin-Song Luo; Tianyu Gu; Yong Yang; Zhenhua Zhang
Source:
Plant molecular biology 2019 v.100 no.4-5 pp. 561-569
ISSN:
0167-4412
Subject:
Arabidopsis thaliana; Escherichia coli; apoplast; bioinformatics; cadmium; cell walls; chelation; cytoplasm; genes; heavy metals; metal tolerance; parenchyma (plant tissue); protein secretion; signal peptide; vascular bundles; yeasts
Abstract:
... KEY MESSAGE: Plant defensin AtPDF2.6 is not secreted to the apoplast and localized in cytoplasm. AtPDF2.6 is mainly expressed in root vascular bundles of xylem parenchyma cell, and significantly induced by Cd stress. AtPDF2.6 detoxicate cytoplasmic Cd via chelation, thus enhanced Cd tolerance in Arabidopsis. In order to detoxify the heavy metal cadmium (Cd), plants have evolved several mechanisms, ...
DOI:
10.1007/s11103-019-00878-y

36. A novel cold-adapted nitroreductase from Psychrobacter sp. ANT206: Heterologous expression, characterization, and nitrobenzene reduction capacity

Author:
Yifan Wang; Quanfu Wang
Source:
Enzyme and microbial technology 2019 pp. -
ISSN:
0141-0229
Subject:
Escherichia coli; Psychrobacter; catalytic activity; genes; heterologous gene expression; hydrogen; nitrobenzenes; nitroreductases; salt tolerance; sea ice; technology; temperature; wastewater; Antarctic region
Abstract:
... Widely used in multiple industrial processes, nitro-aromatic compounds, especially nitrobenzene, in low temperature environment are considered as heavy pollutants. Among the disposal methods, the bioreduction method has attracted much attention. In this study, a novel cold-adapted nitroreductase gene (psntr) was cloned from Antarctic sea-ice bacteria Psychrobacter sp. ANT206. The psntr gene was 81 ...
DOI:
10.1016/j.enzmictec.2019.109434

37. A novel cold-adapted β-galactosidase from Alteromonas sp. ML117 cleaves milk lactose effectively at low temperature

Author:
Congyu Yao; Jingjing Sun; Wei Wang; Zhiwei Zhuang; Junzhong Liu; Jianhua Hao
Source:
Process biochemistry 2019 v.82 pp. 94-101
ISSN:
1359-5113
Subject:
Alteromonas; Escherichia coli; aquatic bacteria; beta-galactosidase; ethanol; gel chromatography; genes; glycosides; hydrolysis; industrial applications; lactose; milk; pH; polyacrylamide gel electrophoresis; sodium chloride; temperature
Abstract:
... A novel β-galactosidase gene (Bgal) was cloned from the marine bacterium Alteromonas sp. ML117. Bgal from Alteromonas sp. ML117 was heterologously expressed in Escherichia coli BL21 (DE3) cells to study the enzymatic properties of the recombinant β-galactosidase. Using gel-filtration chromatography and SDS-PAGE, the recombinant enzyme was identified to be a tetrameric enzyme that belonged to the g ...
DOI:
10.1016/j.procbio.2019.04.016

38. A novel controlled release system based on Tragacanth nanofibers loaded Peppermint oil

Author:
Soraya Ghayempour; Majid Montazer
Source:
Carbohydrate polymers 2019 v.205 pp. 589-595
ISSN:
0144-8617
Subject:
Escherichia coli; Fourier transform infrared spectroscopy; Staphylococcus aureus; antibacterial properties; emulsions; fibroblasts; humans; nanofibers; peppermint oil
Abstract:
... In this paper, a novel controlled release system based on Tragacanth nanofibers loaded with Peppermint oil is introduced by using a sonochemical/microemulsion method. The synthesized Tragacanth nanofibers showed a one-dimensional shape with 58 nm thickness and relatively smooth surface. Also, the loading of Peppermint oil into the synthesized nanofibers confirmed with FT-IR spectra. Further, a rel ...
DOI:
10.1016/j.carbpol.2018.10.078

39. A novel endo-β-1,4-xylanase GH30 from Dickeya dadantii DCE-01: Clone, expression, characterization, and ramie biological degumming function

Author:
Ruijun Wang; Zhengchu Liu; Lifeng Cheng; Shengwen Duan; Xiangyuan Feng; Ke Zheng; Yi Cheng; Jie Zeng
Source:
Textile research journal 2019 v.89 no.4 pp. 463-472
ISSN:
1746-7748
Subject:
Dickeya dadantii; Escherichia coli; calcium; catalytic activity; copper; degumming; enzyme activity; fabrics; genes; glycosides; heterologous gene expression; hydrolysis; magnesium; nucleotides; pH; potassium; ramie; textile fibers; xylan; xylanases
Abstract:
... Xylanase plays an important role in the hydrolysis of hemicellulose and has gained much attention in the field of biological degumming. The research for xylanases with cellulase-free and high activity for biological degumming has intensified in recent years. In the present research, heterologous expression of a novel endo-β-1,4-xylanase (GH30) from Dickeya dadantii DCE-01 in Escherichia coli BL21 ...
DOI:
10.1177/0040517517748511

40. A novel method of producing the pharmaceutical intermediate (R)-2-chloromandelic acid by bioconversion

Author:
Ei-Tora Yamamura; Shinji Kita
Source:
Bioscience, biotechnology, and biochemistry 2019 v.83 no.2 pp. 309-317
ISSN:
1347-6947
Subject:
Escherichia coli; Exophiala dermatitidis; biotechnology; biotransformation; esterases; genes; pH; pharmaceutical industry; temperature
Abstract:
... (R)-2-Chloromandelic acid (R­CM) is one of the chiral building blocks used in the pharmaceutical industry. As a result of screening for microorganisms that asymmetrically hydrolyze racemic 2­chloromandelic acid methyl ester (CMM), Exophiala dermatitidis NBRC6857 was found to produce R­CM at optical purity of 97% ee. The esterase that produces R­CM, EstE, was purified from E. dermatitidis NBRC6857, ...
DOI:
10.1080/09168451.2018.1536517

41. A novel molecular quantitative method for rapid and sensitive detection of Escherichia coli from roof-harvested rainwater

Author:
Rehan Ahmed Deshmukh; Sunil Bhand; Utpal Roy
Source:
Analytical methods 2019 v.11 no.25 pp. 3155-3167
ISSN:
1759-9679
Subject:
DNA; Enterobacter cloacae; Escherichia coli; Escherichia hermannii; Pseudomonas mosselii; agar; analytical methods; detection limit; genes; harvesting; microbiological quality; quantitative analysis; quantitative polymerase chain reaction; rain; rapid methods; ribosomal RNA; sequence analysis
Abstract:
... The harvesting of roof-top rainwater is commonly practiced either for potable or other household purposes. We report a rapid method for the concentration of microbial cells coupled with a DNA extraction procedure that we subjected to xanQ-based quantitative PCR (abbreviated as CoDEX-qPCR, [concentration of microbial cells, DNA extraction and xanQ-based qPCR]) which purports the sensitive detection ...
DOI:
10.1039/c9ay00587k

42. A novel neutral and thermophilic endoxylanase from Streptomyces ipomoeae efficiently produced xylobiose from agricultural and forestry residues

Author:
Liang Xian; Zhong Li; Ai-Xing Tang; Yi-Min Qin; Qing-Yun Li; Hai-Bo Liu; You-Yan Liu
Source:
Bioresource technology 2019 v.285 pp. 121293
ISSN:
0960-8524
Subject:
Cunninghamia lanceolata; Escherichia coli; Phyllostachys edulis; Streptomyces ipomoeae; cassava; corn cobs; endo-1,4-beta-xylanase; genes; industry; pH; prebiotics; temperature; xylan
Abstract:
... Endoxylanases capable of producing high ratios of xylobiose from agricultural and forestry residues in neutral and high temperature conditions are attractive for the prebiotic and alternative sweetener industries. In this study, a putative glycosyl hydrolase gene from Streptomyces ipomoeae was cloned and expressed in Escherichia coli. The recombinant enzyme, named as SipoEnXyn10A, hydrolyzed beech ...
DOI:
10.1016/j.biortech.2019.03.132

43. A novel thermostable and efficient Class II glucose isomerase from the thermophilic Caldicoprobacter algeriensis: Biochemical characterization, molecular investigation, and application in High Fructose Syrup production

Author:
Sawssan Neifar; Hajer Ben Hlima; Sonia Mhiri; Monia Mezghani; Khelifa Bouacem; Adel Hadj Ibrahim; Bassem Jaouadi; Amel Bouanane-Darenfed; Samir Bejar
Source:
International journal of biological macromolecules 2019 v.129 pp. 31-40
ISSN:
0141-8130
Subject:
Escherichia coli; active sites; catalytic activity; fructose; genes; glucose; half life; hydrogen bonding; isomerases; molecular models; pH; polypeptides; serine; syrups; thermal stability
Abstract:
... A novel glucose isomerase gene from the thermophilic Caldicoprobacter algeriensis, encoding a polypeptide of 438 residues, was identified, cloned and successfully expressed in E. coli. The purified enzyme (GICA) was a homotetramer of about 200 kDa displaying the highest activity at pH 7.0 and 90 °C and retaining 97% of its maximum activity at pH 6.5. The enzyme showed an excellent thermostability ...
DOI:
10.1016/j.ijbiomac.2019.01.150

44. A pathogenesis-related protein 10 gene PnPR10–3 was involved in molecular interaction between Panax notoginseng and Fusarium solani

Author:
Bifeng Tang; Xin Li; Limei Pu; Qin Zhao; Xiuming Cui; Feng Ge; Diqiu Liu
Source:
Australasian plant pathology 2019 v.48 no.5 pp. 447-456
ISSN:
0815-3191
Subject:
Allium cepa; Escherichia coli; Fusarium solani; Nicotiana tabacum; Panax notoginseng; affinity chromatography; cytoplasm; enzyme activity; gene expression; gene expression regulation; genes; herbal medicines; hydrogen peroxide; onions; pathogenesis-related proteins; pathogens; quantitative polymerase chain reaction; recombinant proteins; reverse transcriptase polymerase chain reaction; ribonucleases; tobacco; transgenic plants; China
Abstract:
... Pathogenesis-related proteins (PRs) are a great protein superfamily in plants and play important roles during plants respond to a variety of pathogens and environmental stress. In the present study, a PR10 gene, PnPR10–3, was isolated from Panax notoginseng which is a kind of transitional herb medicine in China. The expression pattern of PnPR10–3 gene after inoculation with Fusarium solani as well ...
DOI:
10.1007/s13313-019-00644-0

45. A phenotypic screening bioassay for Escherichia coli stress and antibiotic responses based on Fourier‐transform infrared (FTIR) spectroscopy and multivariate analysis

Author:
B. Ribeiro da Cunha; L.P. Fonseca; C.R.C. Calado
Source:
Journal of applied microbiology 2019 v.127 no.6 pp. 1776-1789
ISSN:
1364-5072
Subject:
Escherichia coli; Fourier transform infrared spectroscopy; antibiotics; bacterial growth; bioassays; ethanol; exposure duration; multivariate analysis; nutrient content; phenotype; principal component analysis; screening; sodium hydroxide; sodium hypochlorite; stress response
Abstract:
... AIMS: To develop and optimize a Fourier‐transform infrared spectroscopy (FTIRS) phenotypic screening bioassay for stress responses, regarding the effect of nutrient content, bacterial growth phase and stress agent exposure time. METHODS AND RESULTS: A high‐throughput FTIRS bioassay was developed to distinguish the stress responses of Escherichia coli to sodium hydroxide, hydrochloric acid, sodium ...
DOI:
10.1111/jam.14429

46. A potent chitin-hydrolyzing enzyme from Myrothecium verrucaria affects growth and development of Helicoverpa armigera and plant fungal pathogens

Author:
Ravindra P. Vidhate; Amey J. Bhide; Sushama M. Gaikwad; Ashok P. Giri
Source:
International journal of biological macromolecules 2019 v.141 pp. 517-528
ISSN:
0141-8130
Subject:
Bipolaris sorokiniana; Escherichia coli; Helicoverpa armigera; Myrothecium verrucaria; Ustilago zeae; antifeedants; antifungal properties; bioassays; biological control; catalytic activity; chitin; chitinase; chitooligosaccharides; hydrolysis; insect pests; mortality; mycelium; open reading frames; pH; pest management; plant pathogenic fungi; polymerization; sequence alignment; temperature
Abstract:
... Chitin, a crucial structural and functional component of insects and fungi, serves as a target for pest management by utilizing novel chitinases. Here, we report the biocontrol potential of recombinant Myrothecium verrucaria endochitinase (rMvEChi) against insect pest and fungal pathogens. A complete ORF of MvEChi (1185 bp) was cloned and heterologously expressed in Escherichia coli. Structure bas ...
DOI:
10.1016/j.ijbiomac.2019.09.031

47. A proposed mechanism of action of textile/Al₂O₃–TiO₂ bimetal oxide nanocomposite as an antimicrobial agent

Author:
Shokoh Parham; Dedy H. B. Wicaksono; Hadi Nur
Source:
The journal of the Textile Institute 2019 v.110 no.5 pp. 791-798
ISSN:
1754-2340
Subject:
Escherichia coli; Fourier transform infrared spectroscopy; anti-infective agents; antimicrobial properties; bacteria; biochemical compounds; cell walls; ethanolamine; fabrics; fibroblasts; free radicals; humans; hydrogen; mechanism of action; models; nanocomposites; nanoparticles; oxidation; reactive oxygen species; reflectance; tissue culture; toxicity; ultraviolet-visible spectroscopy
Abstract:
... The textile/Al₂O₃–TiO₂ bimetal oxide nanocomposite has been developed as an antimicrobial agent, but its antimicrobial mechanism has still not been clarified. The Al₂O₃–TiO₂ bimetal oxide nanoparticle has been reported as a radical scavenger. This study focuses on investigating the antimicrobial mechanism of the textile/Al₂O₃–TiO₂ bimetal oxide nanocomposite against Escherichia coli and their inte ...
DOI:
10.1080/00405000.2018.1526445

48. A rapid method of whole cell sample preparation for scanning electron microscopy using neodymium chloride

Author:
Ivan Novikov; Igor Chebotar
Source:
Micron 2019 pp. 102687
ISSN:
0968-4328
Subject:
Candida albicans; Escherichia coli; Staphylococcus epidermidis; Streptococcus pneumoniae; cell nucleolus; cost effectiveness; cytoskeleton; humans; lead; lead acetate; light microscopy; mitochondria; neodymium; rapid methods; scanning electron microscopy; staining; tomography
Abstract:
... The quality of electron microscopy (EM) visualization of biological objects is constantly improving, primarily with the usage of more complex technologies, such as serial block-face scanning electron microscopy (SEM), focused ion beam scanning electron microscopy, and array tomography. Here we suggest a new rapid method of whole cell sample preparation for scanning EM using neodymium chloride trea ...
DOI:
10.1016/j.micron.2019.102687

49. A rapid tag-free identification of Escherichia coli antibiotic-resistant isolates using Raman scattering

Author:
Soogeun Kim; Seung Ho Lee; Young Jin Kim; Hee Joo Lee; Samjin Choi
Source:
Analytical methods 2019 v.11 no.42 pp. 5381-5387
ISSN:
1759-9679
Subject:
Escherichia coli; Raman spectroscopy; antibiotic resistance; biosensors; food safety; public health; rapid methods; standard deviation; virulent strains
Abstract:
... Rapid and accurate detection of pathogenic bacteria is a critical issue for public health and food safety. We introduce a rapid tag-free identification method for subtyping clinically relevant human blood-cultured Escherichia coli (E. coli) colonies. Gold-deposited surface-enhanced Raman scattering (SERS)-functionalized substrates were used to identify seven E. coli isolates. The method achieved a ...
DOI:
10.1039/c9ay01713e

50. A real-time-based in vitro assessment of the oxidative antimicrobial mechanisms of the myeloperoxidase-hydrogen peroxide-halide system

Author:
Janne Atosuo; Eetu Suominen
Source:
Molecular immunology 2019 v.116 pp. 38-44
ISSN:
0161-5890
Subject:
Escherichia coli; antimicrobial properties; bioluminescence; cell free system; chlorides; chlorination; hydrogen peroxide; hypochlorites; in vitro studies; mammals; microorganisms; myeloperoxidase; neutrophils; phagocytosis; respiratory burst
Abstract:
... Mammals have evolved a special cellular mechanism for killing invading microbes, which is called the phagocytosis. Neutrophils are the first phagocytosing cells that migrate into the site of infection. In these cells, hypochlorite (HOCl) and other hypohalites, generated in the myeloperoxidase (MPO)-hydrogen peroxide (H₂O₂)-halide system is primarily responsible for oxidative killing. Here, we pres ...
DOI:
10.1016/j.molimm.2019.09.017

51. A rugged, self-sterilizing antimicrobial copper coating on ultra-high molecular weight polyethylene: a preliminary study on the feasibility of an antimicrobial prosthetic joint material

Author:
Ke Wu; Samuel P. Douglas; Gaowei Wu; Alexander J. MacRobert; Elaine Allan; Caroline E. Knapp; Ivan P. Parkin
Source:
Journal of materials chemistry B 2019 v.7 no.20 pp. 3310-3318
ISSN:
2050-7518
Subject:
Escherichia coli; Staphylococcus aureus; aerosols; antibacterial properties; coatings; copper; molecular weight; polyethylene; prostheses; temperature; vapors
Abstract:
... We report here for the first time how the combination of a precursor solution and low temperature (170 °C) aerosol assisted chemical vapour deposition were used to bond a copper coating to ultra-high molecular weight polyethylene (UHMWPE) and promote robustness. This metallic thin film remained intact on the UHMWPE substrate after the Scotch tape test and showed notable wear-resistance after 10 cy ...
DOI:
10.1039/c9tb00440h

52. A salicylic acid carboxyl methyltransferase-like gene LcSAMT from Lycium chinense, negatively regulates the drought response in transgenic tobacco

Author:
Gang Wang; Qian Li; Chang Wang; Chao Jin; Jing Ji; Chunfeng Guan
Source:
Environmental and experimental botany 2019 pp. 103833
ISSN:
0098-8472
Subject:
Escherichia coli; Lycium chinense; S-adenosylmethionine; abscisic acid; catalase; chlorophyll; crop yield; crops; drought; drought tolerance; environmental factors; gene overexpression; genes; growth and development; leaves; messenger RNA; methyl salicylate; methylation; methyltransferases; oxidative stress; photosynthesis; plant development; plant growth; proline; salicylic acid; superoxide dismutase; tobacco; transgenic plants; water stress
Abstract:
... Drought is the main environmental factor which limits growth, photosynthesis and yield of agricultural crops worldwide. Salicylic acid (SA) is a phenolic compound involved in plant growth and development. Salicylic acid carboxyl methyltransferase (SAMT) can catalyze the methylation of SA with S-adenosyl-L-methionine as the methyl donor to form methyl salicylate (MeSA). However, the relations betwe ...
DOI:
10.1016/j.envexpbot.2019.103833

53. A small cysteine‐rich protein from two kingdoms of microbes is recognized as a novel pathogen‐associated molecular pattern

Author:
Jiajun Nie; Zhiyuan Yin; Zhengpeng Li; Yuxing Wu; Lili Huang
Source:
new phytologist 2019 v.222 no.2 pp. 995-1011
ISSN:
0028-646X
Subject:
Agrobacterium radiobacter; Escherichia coli; Nicotiana benthamiana; Phytophthora capsici; Sclerotinia sclerotiorum; Valsa mali; cell death; conidiation; elicitors; fungi; gene deletion; gene silencing; pathogen-associated molecular patterns; pathogens; recombinant proteins; screening; virulence
Abstract:
... Pathogen‐associated molecular patterns (PAMPs) are conserved molecules that are crucial for normal life cycle of microorganisms. However, the diversity of microbial PAMPs is little known. During screening of cell‐death‐inducing factors from the necrotrophic fungus Valsa mali, we identified a novel PAMP VmE02 that is widely spread in oomycetes and fungi. Agrobacterium tumefaciens‐mediated transient ...
DOI:
10.1111/nph.15631

54. A step forward: Compatible and dual-inducible expression vectors for gene co-expression in Corynebacterium glutamicum

Author:
Rahul Gauttam; Christian Desiderato; Lisa Jung; Adnan Shah; Bernhard J. Eikmanns
Source:
Plasmid 2019 v.101 pp. 20-27
ISSN:
0147-619X
Subject:
Corynebacterium glutamicum; Escherichia coli; Gram-positive bacteria; amino acids; enzymes; gene overexpression; genes; genetic vectors; metabolic engineering; organic acids and salts; plasmids
Abstract:
... The Gram-positive bacterium Corynebacterium glutamicum represents a promising platform for the production of amino acids, organic acids, and other bio-products. However, the availability of only few expression vectors limits its use for production purposes, using metabolic engineering approaches when co-expression of several target genes is desired. To widen the scope for co-expression, the pCG1/p ...
DOI:
10.1016/j.plasmid.2018.12.004

55. A superoxide dismutase (MnSOD) with identification and functional characterization from the freshwater mussel Cristaria plicata

Author:
Wanying Yang; Wenxiu Liu; Chungen Wen; Baoqing Hu; Shaoqing Jian; Yang Gang
Source:
Fish & shellfish immunology 2019 v.91 pp. 180-187
ISSN:
1050-4648
Subject:
Escherichia coli; amino acid sequences; amino acids; complementary DNA; enzyme activity; enzyme stability; freshwater mussels; gene expression; gene expression regulation; gills; hemocytes; hepatopancreas; immunology; manganese; messenger RNA; microcystins; muscles; open reading frames; pH; plasmids; quantitative polymerase chain reaction; recombinant proteins; sequence homology; shellfish; superoxide dismutase; temperature; urea
Abstract:
... Manganese superoxide dismutase (MnSOD) is a sort of important metalloenzyme that can catalyze ROS in the organisms. In this study, MnSOD cDNA of C. plicata, designated as CpMnSOD (accession no. MK465057), was cloned from hemocytes. The full-length cDNA of MnSOD was 1096 bp with a 672 bp open reading frame encoding 223 amino acids. The deduced amino acid sequence contained a mitochondrial-targeting ...
DOI:
10.1016/j.fsi.2019.04.307

56. A supersensitive MSPQC bacterium sensor based on 16S rRNA and “DNA-RNA switch”

Author:
Ye Feng; Xiaoqing Zhang; Lingling Su; Yilin Zhang; Fengjiao He
Source:
Biosensors & bioelectronics 2019 v.138 pp. 111302
ISSN:
0956-5663
Subject:
Escherichia coli; Listeria innocua; Pseudomonas aeruginosa; Salmonella Enteritidis; Staphylococcus aureus; bacteria; biomarkers; biosensors; detection limit; electrodes; monitoring; public health; public safety; quartz; ribosomal RNA
Abstract:
... The early detection of bacterium plays a significant role in addressing serious public health issues. In this paper, a supersensitive multichannel series piezoelectric quartz crystal (MSPQC) sensor of bacterium based on 16S rRNA and “DNA-RNA switch” was constructed. The fragment in specific region of 16S rRNA was used as the biomarker of bacterium to ensure high specificity and to achieve the accu ...
DOI:
10.1016/j.bios.2019.05.007

57. A two-stage glycine supplementation strategy enhances the extracellular expression of sortase A in Escherichia coli

Author:
Haofei Hong; Xinrui Zhao; Zhimeng Wu
Source:
Process biochemistry 2019 v.76 pp. 11-17
ISSN:
1359-5113
Subject:
Escherichia coli; additives; cell growth; enzyme activity; fermentation; industrial applications; protein engineering; signal peptide; sortase A
Abstract:
... Sortase A (SrtA) is a transpeptidase widely used in protein engineering. In this study, the enhancement of the extracellular expression of SrtA in Escherichia coli was investigated using a combined strategy based on the PelB signal peptide and chemical additives. First, glycine was identified to be the best additive for promoting the release of SrtA from the periplasm to the medium. Then, the effe ...
DOI:
10.1016/j.procbio.2018.09.022

58. A zwitterionic interpenetrating network for improving the blood compatibility of polypropylene membranes applied to leukodepletion

Author:
Cheng-Chi Lien; Po-Ju Chen; Antoine Venault; Shuo-Hsi Tang; Ying Fu; Gian Vincent Dizon; Pierre Aimar; Yung Chang
Source:
Journal of membrane science 2019 v.584 pp. 148-160
ISSN:
0376-7388
Subject:
Escherichia coli; adsorption; artificial membranes; biocompatibility; biofouling; blood platelets; composite polymers; contact angle; cross-linking reagents; deformation; erythrocytes; ethylenediamines; fibrinogen; filters; filtration; hydrophilicity; leukocytes; polypropylenes; zwitterions
Abstract:
... Although widely used in blood-contacting devices, polypropylene (PP) membranes are prone to biofouling by plasma proteins and blood cells. The present study explores the effect of a surface zwitterionization process on the improvement of the biofouling resistance of PP membranes for leukocyte reduction filters. The modification strategy consists in forming an interpenetrating network of poly(glyci ...
DOI:
10.1016/j.memsci.2019.04.056

59. Acetic acid is key for synergetic hydrogen production in Chlamydomonas-bacteria co-cultures

Author:
Neda Fakhimi; Alexandra Dubini; Omid Tavakoli; David González-Ballester
Source:
Bioresource technology 2019 v.289 pp. 121648
ISSN:
0960-8524
Subject:
Chlamydomonas reinhardtii; Escherichia coli; Pseudomonas putida; Rhizobium etli; acetic acid; algae; bacteria; coculture; gas production (biological); glucose; hydrogen; hydrogen production; hypoxia; industrial wastes
Abstract:
... This study is a proof of concept for the synergetic biohydrogen production in alga-bacteria co-cultures. Algal hydrogen photoproduction was obtained in sugar-containing media only when the green alga Chlamydomonas reinhardtii was co-cultured with Pseudomonas putida (40.8 ml H2·L−1), Escherichia coli (35.1 ml H2·L−1) and Rhizobium etli (16.1 ml H2·L−1). Hydrogen photo-production in these co-culture ...
DOI:
10.1016/j.biortech.2019.121648

60. Activation of Alternative Respiration with Internal Electron Acceptor during Anaerobic Glucose Utilization in Escherichia coli Strains with Impaired Fermentation Ability

Author:
A. Yu. Skorokhodova; A. V. Sukhozhenko; A. Yu. Gulevich; V. G. Debabov
Source:
Applied biochemistry and microbiology 2019 v.55 no.9 pp. 870-876
ISSN:
0003-6838
Subject:
Escherichia coli; alanine; biosynthesis; chemical reduction; fermentation; glucose; lactate dehydrogenase; lactic acid; pyruvic acid; tricarboxylic acid cycle
Abstract:
... The activation of alternative respiration with an internal electron acceptor during anaerobic glucose utilization in E. coli strains with impaired fermentation ability has been studied. It was found out that respiration processes utilizing pyruvic acid as an endogenous electron acceptor can markedly contribute to the maintenance of the anaerobic redox balance in E. coli strains deficient in mixed ...
DOI:
10.1134/S0003683819090072

61. Activation of colanic acid biosynthesis linked to heterologous expression of the polyhydroxybutyrate pathway in Escherichia coli

Author:
Haiying Wu; Shangwei Chen; Minghua Ji; Qiao Chen; Jiping Shi; Junsong Sun
Source:
International journal of biological macromolecules 2019 v.128 pp. 752-760
ISSN:
0141-8130
Subject:
Escherichia coli; NADP (coenzyme); acetyl coenzyme A; biosynthesis; gene overexpression; genomics; glucose; growth performance; heterologous gene expression; loci; mutation; pH; plasmids; polyhydroxybutyrate; regulon; temperature; transcription (genetics)
Abstract:
... The biosynthesis of colanic acid (CA) in Escherichia coli was known to be activated during growth at low temperature using sub-optimal medium. However, in this study, an E. coli transformant S173-H (S17-3 with plasmid pBhya-CAB) was found to be able to excrete high amount of CA (10.39 g/L) in glucose supplemented Luria-Bertani medium (LBG) when growing at 37 °C. Inoculation of cells in low pH medi ...
DOI:
10.1016/j.ijbiomac.2019.02.004

62. Addition of Wakame seaweed (Undaria pinnatifida) stalk to animal feed enhances immune response and improves intestinal microflora in pigs

Author:
Tomoyuki Shimazu; Liushiqi Borjigin; Kazuo Katoh; Sang‐gun Roh; Haruki Kitazawa; Keietsu Abe; Yoshihito Suda; Hayato Saito; Hiroshi Kunii; Ken Nihei; Yoshinobu Uemoto; Hisashi Aso; Keiichi Suzuki
Source:
Animal science journal = 2019 v.90 no.9 pp. 1248-1260
ISSN:
1344-3941
Subject:
Escherichia coli; Lactobacillus; Peyer's patches; Toll-like receptors; Undaria pinnatifida; additives; antibiotics; cytokines; feed intake; feeds; finishing; gene expression; immune response; intestinal microorganisms; intestines; macroalgae; natural killer cells; swine; weight gain
Abstract:
... This study was conducted to evaluate the effects of supplementation of Wakame seaweed stalks on the immunity and intestinal microflora of pigs. Three separate experiments were performed: Relatively young (start at 20–30 kg; Experiments 1 and 2) and fattening period (70 kg; Experiment 3). All pigs (including the control group) were fed the same commercial feed, free from antibiotic additives, but i ...
DOI:
10.1111/asj.13274

63. Adhesion mediated transport of bacterial pathogens in saturated sands coated by phyllosilicates and Al-oxides

Author:
Zhi-neng Hong; Jun Jiang; Jiu-yu Li; Ren-kou Xu; Jing Yan
Source:
Colloids and surfaces 2019 v.181 pp. 215-225
ISSN:
0927-7765
Subject:
Escherichia coli; Staphylococcus aureus; aluminum oxide; bacteria; chemical interactions; coatings; hydrophobicity; ionic strength; kaolinite; montmorillonite; pH; pathogens; porous media; prediction; quartz; soil
Abstract:
... The current knowledge of bacterial migration is mainly derived from work using bare or Fe-coated quartz sands as porous media. However, mineral coatings on quartz by phyllosilicates and Al-oxides prevail in natural soils, and their effect on bacterial transport remains unknown. Herein, we systematically explored the transport of two bacterial pathogens (Escherichia coli and Staphylococcus aureus) ...
DOI:
10.1016/j.colsurfb.2019.05.044

64. Adsorption of extracellular polymeric substances from two microbes by TiO2 nanoparticles

Author:
Xuan Gao; Ayyaraju Middepogu; Rui Deng; Jingfu Liu; Zhineng Hao; Daohui Lin
Source:
Science of the total environment 2019 v.694 pp. 133778
ISSN:
0048-9697
Subject:
Chlorella pyrenoidosa; Escherichia coli; adsorption; mass spectrometry; microorganisms; moieties; molecular weight; nanoparticles; polymers; polysaccharides; surface area; thermodynamics; titanium dioxide; toxicity
Abstract:
... Extracellular polymeric substance (EPS) secreted by microbes can interact with nanoparticles (NPs) and thus influence environmental behavior and toxicity of NPs. The adsorption of EPSs from two species of microbes (Escherichia coli and Chlorella pyrenoidosa) by four types of titanium dioxide nanoparticles (nTiO2) (5, 10, and 40 nm anatase nTiO2 and 25 nm rutile nTiO2) were therefore specifically i ...
DOI:
10.1016/j.scitotenv.2019.133778

65. Advanced oxidation processes on doxycycline degradation: monitoring of antimicrobial activity and toxicity

Author:
Mylena Spina-Cruz; Milena Guedes Maniero; José Roberto Guimarães
Source:
Environmental science and pollution research international 2019 v.26 no.27 pp. 27604-27619
ISSN:
0944-1344
Subject:
Bacillus subtilis; Escherichia coli; Vibrio fischeri; acute toxicity; antimicrobial properties; aquatic bacteria; bioluminescence; doxycycline; drugs; hydrogen peroxide; monitoring; oxidation; ozonation; ozone; pH; peroxidation; photolysis; toxic substances; toxicity testing
Abstract:
... Advanced oxidation processes (AOPs) have been highly efficient in degrading contaminants of emerging concern (CEC). This study investigated the efficiency of photolysis, peroxidation, photoperoxidation, and ozonation at different pH values to degrade doxycycline (DC) in three aqueous matrices: fountain, tap, and ultrapure water. More than 99.6% of DC degradation resulted from the UV/H₂O₂ and ozona ...
DOI:
10.1007/s11356-018-2149-1

66. Affiliation and disease risk: social networks mediate gut microbial transmission among rhesus macaques

Author:
Krishna N. Balasubramaniam; Brianne A. Beisner; Josephine A. Hubbard; Jessica J. Vandeleest; Edward R. Atwill; Brenda McCowan
Source:
Animal behaviour 2019 v.151 pp. 131-143
ISSN:
0003-3472
Subject:
Escherichia coli; Macaca mulatta; adults; animal behavior; captive animals; data collection; digestive system; disease control; disease transmission; females; gastrointestinal parasites; intestinal microorganisms; males; pathogens; phylogeny; prediction; risk; social networks
Abstract:
... In social animals, affiliative behaviours bring many benefits, but also costs such as disease risk. The ways in which affiliation may affect the risk of infectious agent transmission remain unclear. Moreover, studies linking variation in affiliative interactions to infectious agent incidence/diversity have speculated that disease transmission may have occurred, rather than revealing that transmiss ...
DOI:
10.1016/j.anbehav.2019.03.009

67. Ag₂S@WS₂ Heterostructure for Rapid Bacteria-Killing Using Near-Infrared Light

Author:
Yunfan Lin; Donglin Han; Yuan Li; Lei Tan; Xiangmei Liu; Zhenduo Cui; Xianjin Yang; Zhaoyang Li; Yanqin Liang; Shengli Zhu; Shuilin Wu
Source:
ACS sustainable chemistry & engineering 2019 v.7 no.17 pp. 14982-14990
ISSN:
2168-0485
Subject:
Escherichia coli; Staphylococcus aureus; antibacterial properties; antibiotics; bacteria; bacterial infections; cell culture; disinfection; drug resistance; electronics; human health; irradiation; nanoparticles; nanosheets; near infrared radiation; photocatalysis; silver sulfide; synergism
Abstract:
... On a global scale, bacterial infection has become a huge threat to human health. Taking into consideration the increasing drug-resistance bacteria, it is urgent to develop novel nonantibiotics strategies to kill bacteria rapidly. Herein, Ag₂S@WS₂ was developed as a new kind of antibacterial agent, where exfoliated WS₂ nanosheets were decorated with Ag₂S nanoparticles through in situ growth. Under ...
DOI:
10.1021/acssuschemeng.9b03287

68. Albumin Broadens the Antibacterial Capabilities of Nonantibiotic Small Molecule-Capped Gold Nanoparticles

Author:
Zhencheng Sun; Wenshu Zheng; Guoshuai Zhu; Jie Lian; Jidong Wang; Ping Hui; Songliang He; Wenwen Chen; Xingyu Jiang
Source:
ACS applied materials & interfaces 2019 v.11 no.49 pp. 45381-45389
ISSN:
1944-8252
Subject:
Escherichia coli; Gram-negative bacteria; Gram-positive bacteria; Pseudomonas aeruginosa; Staphylococcus aureus; abscess; antibacterial properties; antibiotics; bacterial infections; biofilm; bovine serum albumin; mice; minimum inhibitory concentration; multiple drug resistance; nanogold; thiols; toxicity
Abstract:
... Nonantibiotic small molecule-modified gold nanoparticles (Au NPs) show great potential as an alternative for commercial antibiotics, yet their narrow antibacterial spectrum hinders the wide application in clinics. We observe that Au NPs cofunctionalized with both bovine serum albumin (BSA) and 4,6-diamino-2-pyrimidinethiol (DAPT) can generate conjugates (Au_DAPT_BSA) with progressive antimicrobial ...
DOI:
10.1021/acsami.9b15107

69. Alginate films functionalized with silver sulfadiazine-loaded [Mg-Al] layered double hydroxide as antimicrobial wound dressing

Author:
Davi R. Munhoz; Marcela P. Bernardo; João O.D. Malafatti; Francys K.V. Moreira; Luiz H.C. Mattoso
Source:
International journal of biological macromolecules 2019 v.141 pp. 504-510
ISSN:
0141-8130
Subject:
Escherichia coli; Salmonella enterica; Staphylococcus aureus; aluminum; antibiotics; antimicrobial properties; bacterial infections; cytotoxicity; drugs; fibroblasts; glycerol; hydrotalcite; magnesium; mechanical properties; plasticizers; silver; sodium alginate; sulfadiazine; tissue engineering
Abstract:
... Alginate (ALG) is an abundant, biocompatible, regenerative, and nontoxic polysaccharide that has potential applications in tissue engineering. Silver sulfadiazine (SDZ) is a topical antibiotic used to control bacterial infection in burns. Aiming to combine the intrinsic alginate characteristics and silver sulfadiazine antimicrobial properties, hydrotalcite ([Mg-Al]-LDH) was used as a host matrix t ...
DOI:
10.1016/j.ijbiomac.2019.09.019

70. Alginate–pectin co-encapsulation of dextransucrase and dextranase for oligosaccharide production from sucrose feedstocks

Author:
Manisha Sharma; Rajender S. Sangwan; Bhupender S. Khatkar; Sudhir P. Singh
Source:
Bioprocess and biosystems engineering 2019 v.42 no.10 pp. 1681-1693
ISSN:
1615-7591
Subject:
Escherichia coli; Leuconostoc mesenteroides; Streptococcus mutans; bioprocessing; catalytic activity; dextranase; dextransucrase; feedstocks; gene expression; genes; genomics; heat tolerance; heterologous gene expression; molasses; pH; polymerization; prebiotics; storage quality; sucrose; temperature; white sugar
Abstract:
... The genes for dextransucrase and dextranase were cloned from the genomic regions of Leuconostoc mesenteroides MTCC 10508 and Streptococcus mutans MTCC 497, respectively. Heterologous expression of genes was performed in Escherichia coli. The purified enzyme fractions were entrapped in the alginate–pectin beads. A high immobilization yield of dextransucrase (~ 96%), and dextranase (~ 85%) was achie ...
DOI:
10.1007/s00449-019-02164-z

71. Alpha protons as NMR probes in deuterated proteins

Author:
Kumar Tekwani Movellan; Eszter E. Najbauer; Supriya Pratihar; Michele Salvi; Karin Giller; Stefan Becker; Loren B. Andreas
Source:
Journal of biomolecular NMR 2019 v.73 no.1-2 pp. 81-91
ISSN:
0925-2738
Subject:
Escherichia coli; carbon; membrane proteins; protein structure; protonation; stereospecificity; transamination
Abstract:
... We describe a new labeling method that allows for full protonation at the backbone Hα position, maintaining protein side chains with a high level of deuteration. We refer to the method as alpha proton exchange by transamination (α-PET) since it relies on transaminase activity demonstrated here using Escherichia coli expression. We show that α-PET labeling is particularly useful in improving struct ...
DOI:
10.1007/s10858-019-00230-y

72. Amelioration of sperm immobilisation factor-induced infertility by bacterial antigenic determinants cross-reacting with spermatozoa

Author:
Deepali Thaper; Deepak K. Rahi; Vijay Prabha
Source:
Reproduction, fertility, and development 2019 v.31 no.3 pp. 602-612
ISSN:
1031-3613
Subject:
Escherichia coli; Staphylococcus aureus; adenosinetriphosphatase; antibodies; antifertility effect; bacteria; enzyme activity; epitopes; females; fluorescein; humans; mice; molecular mimicry; receptors; spermatozoa; viability
Abstract:
... A strain of Staphylococcus aureus, capable of invitro immobilisation of human and mouse spermatozoa, was already present in our laboratory. Therefore, in the present study, the factor responsible (sperm immobilisation factor, SIF) was isolated and purified. It was found to compromise not only motility, but also viability, morphology and Mg2+-ATPase activity of mouse spermatozoa. Also, SIF (250μgmL ...
DOI:
10.1071/RD18300

73. Amino Acid-Modified Conjugated Oligomer Self-Assembly Hydrogel for Efficient Capture and Specific Killing of Antibiotic-Resistant Bacteria

Author:
Qi Zhao; Yantao Zhao; Zhuanning Lu; Yanli Tang
Source:
ACS applied materials & interfaces 2019 v.11 no.18 pp. 16320-16327
ISSN:
1944-8252
Subject:
Escherichia coli; Staphylococcus epidermidis; antibacterial properties; antibiotic resistance; bacteria; bacterial infections; cell walls; cytotoxicity; disinfection; hospitals; human health; hydrogels; methicillin-resistant Staphylococcus aureus; models; mortality; therapeutics; thiophene
Abstract:
... Bacterial infection is one of main causes that threaten global human health. Especially, antibiotic-resistant bacteria like methicillin-resistant Staphylococcus aureus (MRSA) lead to high mortality rate and more expensive treatment cost. Here, a novel amino-acid-modified conjugated oligomer OTE-d-Phe was synthesized by modifying the side chain of conjugated oligo(thiophene ethynylene) with d-pheny ...
DOI:
10.1021/acsami.9b02643

74. An L-fucose specific lectin from Aspergillus niger isolated from mycotic keratitis patient and its interaction with human pancreatic adenocarcinoma PANC-1 cells

Author:
Narasimhappagari Jagadeesh; Shivakumar Belur; Prajna Hegde; A.S. Kamalanathan; Bale M. Swamy; Shashikala R. Inamdar
Source:
International journal of biological macromolecules 2019 v.134 pp. 487-497
ISSN:
0141-8130
Subject:
Aspergillus niger; Aspergillus oryzae; Escherichia coli; Klebsiella pneumoniae; Pseudomonas aeruginosa; Staphylococcus aureus; adenocarcinoma; antibacterial properties; biofilm; blood groups; carbohydrate content; cornea; electrospray ionization mass spectrometry; erythrocytes; fetuins; fucose; galactose; haptens; heat stability; humans; inhibitory concentration 50; keratitis; lactose; lectins; molecular weight; mucins; pH; patients; polyacrylamide gel electrophoresis; rabbits; virulent strains
Abstract:
... An L-fucose specific lectin from pathogenic fungus Aspergillus niger isolated from the corneal smears of keratitis patient was purified in a single step using mucin coupled sepharose-4B column by 58-fold. The purified lectin, ANL has molecular mass of 30 kDa by SDS–PAGE and 31.6 kDa by ESI-MS. ANL is a glycoprotein with 2.59% carbohydrate. ANL is blood group nonspecific and also agglutinates rabbi ...
DOI:
10.1016/j.ijbiomac.2019.04.192

75. An active packaging film based on yam starch with eugenol and its application for pork preservation

Author:
Junfeng Cheng; Hualin Wang; Shaolei Kang; Li Xia; Suwei Jiang; Minmin Chen; Shaotong Jiang
Source:
Food hydrocolloids 2019 v.96 pp. 546-554
ISSN:
0268-005X
Subject:
Dioscorea polystachya; Escherichia coli; Listeria monocytogenes; Staphylococcus aureus; active food packaging; antibacterial properties; dose response; eugenol; glycerol; hydrocolloids; packaging films; plasticizers; pork; shelf life; sorbitol; starch; yams
Abstract:
... An antibacterial film based on Chinese yam starch (D-sorbitol as plasticizer) incorporated with eugenol (YDE) was prepared for pork preservation. The structure of films was characterized and the evaluation of two plasticizers (glycerol and D-sorbitol) on their mechanical and barrier properties was performed to determine the vehicle of eugenol. The release behavior and antimicrobial activity of YDE ...
DOI:
10.1016/j.foodhyd.2019.06.007

76. An aligned porous electrospun fibrous scaffold with embedded asiatic acid for accelerating diabetic wound healing

Author:
Yiming Han; Yuqi Jiang; You Li; Minna Wang; Tingting Fan; Mingyao Liu; Qinfei Ke; He Xu; Zhengfang Yi
Source:
Journal of materials chemistry B 2019 v.7 no.40 pp. 6125-6138
ISSN:
2050-7518
Subject:
Escherichia coli; Staphylococcus aureus; angiogenesis; chemistry; extracellular matrix; gene expression; gene expression regulation; in vivo studies; inflammation; interleukin-1beta; interleukin-6; macrophages; nanopores; oxidative stress; polylactic acid; tissue repair; tumor necrosis factor-alpha
Abstract:
... The diabetic non-healing wound is one of the most common complications of diabetics. The long-term stimulus of oxidative stress, inflammation and infection caused by the hyperglycemic microenvironment in the wound site always leads to a delayed healing process of the diabetic wound. To address this issue, in this study, we prepared an asiatic acid (AA)-embedded aligned porous poly(l-lactic acid) ( ...
DOI:
10.1039/c9tb01327j

77. An alkaline pectate lyase D from Dickeya dadantii DCE-01: clone, expression, characterization, and potential application in ramie bio-degumming

Author:
Lifeng Cheng; Shengwen Duan; Ke Zheng; Xiangyuan Feng; Qi Yang; Zhiyuan Liu; Zhengchu Liu; Yuande Peng
Source:
Textile research journal 2019 v.89 no.11 pp. 2075-2083
ISSN:
1746-7748
Subject:
Citrus; Dickeya dadantii; Escherichia coli; amino acids; ammonium; bacteria; calcium; catalytic activity; copper; degumming; enzyme activity; esterification; fabrics; gel chromatography; genes; heat tolerance; iron; lead; pH; pectate lyase; pectins; plasmids; polygalacturonase; ramie; temperature; textile fibers; ultrafiltration; zinc
Abstract:
... Pectinase plays a crucial role in ramie bio-degumming. A pectate lyase gene (pel4J4) from the high-efficiency degumming bacteria Dickeya dadantii DCE-01 of bast fibers was cloned and connected to pET28a, and then the recombinant plasmid was successfully transformed into Escherichia coli BL21(DE3). The pectate lyase (Pel4J4) induced was purified by ultrafiltration and Sephadex G-100 gel chromatogra ...
DOI:
10.1177/0040517518790971

78. An alternative strategy for combination therapy: Interactions between polymyxin B and non-antibiotics

Author:
Robin G. Otto; Elke van Gorp; Wendy Kloezen; Joseph Meletiadis; Sanne van den Berg; Johan W. Mouton
Source:
International journal of antimicrobial agents 2019 v.53 no.1 pp. 34-39
ISSN:
0924-8579
Subject:
Acinetobacter baumannii; Escherichia coli; Klebsiella pneumoniae; Pseudomonas aeruginosa; absorbance; agar; antibacterial properties; antibiotic resistance; drugs; infectious diseases; polymyxin B; screening; spironolactone; synergism; therapeutics
Abstract:
... Antimicrobial resistance is increasing and few new antibiotics are in the development pipeline. Alternative strategies to treat infectious diseases, such as combination therapy, are urgently needed. Polymyxin B is a neglected and disused antibiotic with moderate antibacterial activity. In this study, we aimed to find synergistic interactions between polymyxin B and a wide range of non-antibiotics ...
DOI:
10.1016/j.ijantimicag.2018.09.003

79. An approach to recombinantly produce mature grape polyphenol oxidase

Author:
Yu Li; Mark-Anthony McLarin; Martin J. Middleditch; Stuart J. Morrow; Paul A. Kilmartin; Ivanhoe K.H. Leung
Source:
Biochimie 2019 v.165 pp. 40-47
ISSN:
0300-9084
Subject:
Escherichia coli; catalytic activity; catechol oxidase; eukaryotic cells; grapes; polyphenols; prokaryotic cells; ultraviolet-visible spectroscopy
Abstract:
... Polyphenol oxidases (PPOs) are important enzymes that are widely found in both prokaryotes and eukaryotes including grapes. Studies of grape PPO to date have mostly relied on enzymes extracted and purified from plants. In this work, we describe the production of the mature form of Shine Muscat grape PPO by using an Escherichia coli expression system. We have optimised the purification procedure to ...
DOI:
10.1016/j.biochi.2019.07.002

80. An improved plasmid size standard, 39R861+

Author:
Robert A. Moran; Sashindran Anantham; Ruth M. Hall
Source:
Plasmid 2019 v.102 pp. 6-9
ISSN:
0147-619X
Subject:
Escherichia coli; genetic conjugation; nucleotide sequences; plasmids
Abstract:
... The four plasmids in E. coli 39R861 have been used as size standards for closed-circular plasmid DNA since the 1980s. However, their sizes had only been estimated. Here, the complete sequence of the 61.3 kb cryptic FII plasmid p39R861–3 is reported, and the sizes of p39R861–4, pSa and NTP168 were determined to be 155.8, 40.1 and 6.8 kb, respectively. An improved size standard, 39R861+, was created ...
DOI:
10.1016/j.plasmid.2019.01.002

81. An integrated quantitative proteomic and metabolomics approach to reveal the negative regulation mechanism of LamB in antibiotics resistance

Author:
Wanxin Li; Guibin Wang; Song Zhang; Yuying Fu; Yuhang Jiang; Xiaojun Yang; Xiangmin Lin
Source:
Journal of proteomics 2019 v.194 pp. 148-159
ISSN:
1874-3919
Subject:
DNA replication; Escherichia coli; amino acids; antibiotic resistance; bioinformatics; biosynthesis; ciprofloxacin; gas chromatography-mass spectrometry; gene ontology; genes; gluconeogenesis; glycolysis; liquid chromatography; maltose; metabolites; metabolomics; multiple drug resistance; mutants; pentose phosphate cycle; porins; protein synthesis; proteomics; tandem mass spectrometry; translation (genetics); tricarboxylic acid cycle
Abstract:
... Previously, a maltose-specific channel porin, LamB was found to be associate with multi-drug resistance in a lamB deleted strain, but the exact mechanisms require further elucidation. Herein, differential protein expression between the Escherichia coli mutant strain ΔlamB and the wild type strain BW25113, with and without ciprofloxacin (CFLX), was identified using iTRAQ based liquid chromatography ...
DOI:
10.1016/j.jprot.2018.11.022

82. An oral double-targeted DNA vaccine induces systemic and intestinal mucosal immune responses and confers high protection against Vibrio mimicus in grass carps

Author:
Ji Cao; Xin-Chi Zhu; Xin-Yuan Liu; Kang Yuan; Jia-Jun Zhang; Hui-Hui Gao; Jin-Nian Li
Source:
Aquaculture 2019 v.504 pp. 248-259
ISSN:
0044-8486
Subject:
Ctenopharyngodon idella; DNA; Escherichia coli; Vibrio mimicus; animal tissues; antibodies; blood serum; digestive tract; disease resistance; enteropathogens; epitopes; fish; gene expression; genes; immunogenicity; intestines; lymphocytes; mucosal immunity; mucus; peptides; proteins; recombinant vaccines; superoxide dismutase; transcription (genetics); vibriosis
Abstract:
... Vibrio mimicus (V. mimicus) is an enteric pathogen causing serious vibriosis in aquatic animals, and intestinal mucosal immunity elicited by oral DNA vaccines plays a vital role in the resistance to the pathogen. Yet, the immunogenicity of oral naked DNA vaccine is poor due to the degradation in the digestive tract. Therefore, it is necessary to use targeted DNA delivery strategy for improving the ...
DOI:
10.1016/j.aquaculture.2019.02.006

83. Analysis and preliminary characterisation of the cytochrome P450 monooxygenases from Frankia sp. EuI1c (Frankia inefficax sp.)

Author:
Ian C.K. Lau; René Feyereisen; David R. Nelson; Stephen G. Bell
Source:
Archives of biochemistry and biophysics 2019 v.669 pp. 11-21
ISSN:
0003-9861
Subject:
Escherichia coli; Frankia; bacteria; biosynthesis; cytochrome P-450; dihydrotestosterone; electron transfer; ferredoxin-NAD+ reductase; ferredoxins; gene duplication; nitrogen fixation; norisoprenoids; progesterone; root nodules; testosterone
Abstract:
... Frankia bacteria are nitrogen fixing species from the Actinobacterium phylum which live on the root nodules of plants. They have been hypothesised to have significant potential for natural product biosynthesis. The cytochrome P450 monooxygenase complement of Frankia sp. EuI1c (Frankia inefficax sp.), which comprises 68 members, was analysed. Several members belonged to previously uncharacterised b ...
DOI:
10.1016/j.abb.2019.05.007

84. Analysis of antibiotic resistance of Escherichia coli isolated from the Yitong River in North-east China

Author:
Yangyang Yu; Xiaolin Zhu; Guanlan Wu; Chengzhi Wang; Xing Yuan
Source:
Frontiers of environmental science & engineering 2019 v.13 no.3 pp. 39
ISSN:
2095-2201
Subject:
Escherichia coli; antibiotic resistance; breeding sites; coliform bacteria; fluoroquinolones; high performance liquid chromatography; livestock; livestock breeding; plate count; pollution; risk; rivers; seasonal variation; spring; sulfonamides; summer; tetracyclines; trimethoprim; China
Abstract:
... The Yitong River is one of the largest secondary tributaries of the Songhua River. The area where the Yitong River flows is densely populated and contains the livestock and poultry breeding areas of northeast China. These areas introduce a high risk of antibiotic contamination. In this study, the concentrations of four types of typical antibiotics including quinolones, tetracyclines, sulfonamides, ...
DOI:
10.1007/s11783-019-1123-6

85. Analysis of milk-derived isolates of E. coli indicating drug resistance in central Ethiopia

Author:
Yohannes Equar Messele; Reta Duguma Abdi; Desiye Tesfaye Tegegne; Shelema Kelbesa Bora; Mosisa Dire Babura; Bezina Arega Emeru; Gebremeskel Mamu Werid
Source:
Tropical animal health and production 2019 v.51 no.3 pp. 661-667
ISSN:
0049-4747
Subject:
California mastitis test; Escherichia coli; ampicillin; antibiotic resistance; bovine mastitis; chloramphenicol; dairy cows; dairy farming; dairy industry; financial economics; gentamicin; milk; multiple drug resistance; phenotype; raw milk; resistance genes; streptomycin; Ethiopia
Abstract:
... Mastitis is one of the most important diseases in dairy cows throughout the world and is responsible for significant economic losses to the dairy industry. This study was performed to characterize the genetic basis of drug resistance in Escherichia coli isolated from cases of clinical and sub-clinical bovine mastitis. A total of 224 California mastitis test (CMT)-positive milk samples were collect ...
DOI:
10.1007/s11250-018-1737-x

86. Analysis of nucleotide pools in bacteria using HPLC-MS in HILIC mode

Author:
Eva Zborníková; Zdeněk Knejzlík; Vasili Hauryliuk; Libor Krásný; Dominik Rejman
Source:
Talanta 2019 v.205 pp. 120161
ISSN:
0039-9140
Subject:
bacteria; bioactive properties; chemical species; cold; detectors; filtration; high performance liquid chromatography; hydrophilic interaction chromatography; mass spectrometry; nucleosides; nucleotides; zwitterions
Abstract:
... Nucleotides, nucleosides and their derivatives are present in all cells at varying concentrations that change with the nutritional, and energetic status of the cell. Precise measurement of the concentrations of these molecules is instrumental for understanding their regulatory effects. Such measurement is challenging due to the inherent instability of these molecules and, despite many decades of r ...
DOI:
10.1016/j.talanta.2019.120161

87. Annual changes in the occurrence of antibiotic-resistant coliform bacteria and enterococci in municipal wastewater

Author:
Kristína Lépesová; Petra Olejníková; Tomáš Mackuľak; Jozef Tichý; Lucia Birošová
Source:
Environmental science and pollution research international 2019 v.26 no.18 pp. 18470-18483
ISSN:
0944-1344
Subject:
Citrobacter; Enterococcus; Escherichia coli; Morganella morganii; antibiotic resistance; antibiotics; biofilm; coliform bacteria; monitoring; multiple drug resistance; municipal wastewater; pollutants; resistance genes; temperature; wastewater treatment; Slovakia
Abstract:
... Wastewater contains subinhibitory concentrations of different micropollutants such as antibiotics that create selective pressure on bacteria. This phenomenon is also caused by insufficient wastewater treatment technology leading to the development and spread of antibiotic-resistant bacteria and resistance genes into the environment. Therefore, this work focused on monitoring of antibiotic-resistan ...
DOI:
10.1007/s11356-019-05240-9

88. Anthropogenic Changes in the Hydrochemical and Sanitary–Microbiological Characteristics of Water Quality in Southern Baikal Tributaries: Listvennichnyi Bay

Author:
V. V. Malnik; O. A. Timoshkin; A. N. Suturin; N. A. Onishchuk; M. V. Sakirko; I. V. Tomberg; A. S. Gorshkova; N. S. Zabanova
Source:
Water resources 2019 v.46 no.5 pp. 748-758
ISSN:
0097-8078
Subject:
Enterococcus; Escherichia coli; Malaya; anthropogenic activities; coastal water; coliform bacteria; heat tolerance; hydrochemistry; phosphates; river water; rivers; surveys; tourists; water quality; Lake Baikal; Russia; United States
Abstract:
... A sanitary–bacteriological and hydrochemical survey of rivers flowing into Lake Baikal near Listvyanka Settl. was carried out in period from 2014 to 2015. Water quality at the mouths of the rivers of Bol’shaya Cheremshanka, Malaya Cheremshanka, and Kamenushka was found not to meet the standards of the normative and technical documentation introduced in Russia and abroad (in the United States and E ...
DOI:
10.1134/S0097807819050154

89. Anthropogenic environmental drivers of antimicrobial resistance in wildlife

Author:
Benjamin M.C. Swift; Malcolm Bennett; Katie Waller; Christine Dodd; Annie Murray; Rachel L. Gomes; Bethan Humphreys; Jon L. Hobman; Michael A. Jones; Sophia E. Whitlock; Lucy J. Mitchell; Rosie J. Lennon; Kathryn E. Arnold
Source:
Science of the total environment 2019 v.649 pp. 12-20
ISSN:
0048-9697
Subject:
Escherichia coli; animal wastes; antibiotic resistance; bacteria; colistin; farms; feces; genes; multiple drug resistance; risk; sewage treatment; wild birds; wildlife
Abstract:
... The isolation of antimicrobial resistant bacteria (ARB) from wildlife living adjacent to humans has led to the suggestion that such antimicrobial resistance (AMR) is anthropogenically driven by exposure to antimicrobials and ARB. However, ARB have also been detected in wildlife living in areas without interaction with humans. Here, we investigated patterns of resistance in Escherichia coli isolate ...
DOI:
10.1016/j.scitotenv.2018.08.180

90. Anti-biofilm efficacy of single and binary treatments based on plant essential oils against Escherichia coli persistent in food-processing facilities

Author:
Daniel Vázquez-Sánchez; Juliana Antunes Galvão; Marina Rodrigues Mazine; Eduardo Micotti da Gloria; Thais Maria Ferreira de Souza Vieira
Source:
Food science and technology international 2019 v.25 no.5 pp. 385-393
ISSN:
1532-1738
Subject:
Escherichia coli; biofilm; disinfectants; environmental impact; essential oils; food industry; food processing; food processing plants; mixing; oils; peracetic acid; plankton; polystyrenes; sanitizing; sodium hypochlorite; stainless steel; thyme
Abstract:
... The efficacy of single and combined treatments based on plant essential oils was investigated against Escherichia coli strains persistent in food-processing facilities. Surface materials (stainless steel and polystyrene), disinfectants (peracetic acid and sodium hypochlorite), and conditions (25 ℃, frequency of sanitizing of 24 h) commonly present in the food industry were also used to reach a mor ...
DOI:
10.1177/1082013219826817

91. Antibacterial Activity and Chemical Composition of Essential Oils from Leaves of Some Aromatic Plants of Philippines

Author:
Adrienne Marrie Bugayong; Philip Cruz; Philip Ian Padilla
Source:
Journal of essential oil-bearing plants 2019 v.22 no.4 pp. 932-946
ISSN:
0976-5026
Subject:
Curcuma longa; Escherichia coli; Lippia; Ocimum tenuiflorum; Philippines; Plectranthus amboinicus; Staphylococcus epidermidis; antibacterial properties; carvacrol; chemical composition; citral; essential oils; steam distillation
Abstract:
... In this present study, the antibacterial activity and the chemical composition of essential oils from the leaves of ten aromatic plants growing in the Philippines were determined. Essential oils were isolated by steam distillation and their antibacterial activities against the gram-positive bacteria (S. aureus, S. epidermidis and E. faecalis) and gram-negative bacteria (P. aeruginosa and E. coli) ...
DOI:
10.1080/0972060X.2019.1682683

92. Antibacterial LDPE/GSE/Mel/ZnONP composite film-coated wrapping paper for convenience food packaging application

Author:
Shiv Shankar; Yeong-Ju Bang; Jong-Whan Rhim
Source:
Food packaging and shelf life 2019 v.22 pp. 100421
ISSN:
2214-2894
Subject:
Citrus paradisi; Escherichia coli; Listeria monocytogenes; antibacterial properties; coatings; coliform bacteria; composite films; contact angle; food packaging; grapefruits; macroalgae; melanin; nanocomposites; nanoparticles; oils; paper; polyethylene; prepared foods; rice; scanning electron microscopy; seed extracts; shelf life; tensile strength; thermal stability; zinc oxide
Abstract:
... Low-density polyethylene (LDPE) based nanocomposite film incorporated with grapefruit seed extract (GSE), melanin nanoparticle (Mel), and zinc oxide nanoparticles (ZnONP) was prepared by the extrusion-blowing method. The UV–vis spectra of LDPE/GSE/Mel/ ZnONP film exhibited the characteristic absorption peak of ZnONP at 370 nm. The UV-barrier, thickness, elongation at break, and thermal stability o ...
DOI:
10.1016/j.fpsl.2019.100421

93. Antibacterial activity of compounds isolated from Caesalpinia coriaria (Jacq) Willd against important bacteria in public health

Author:
Agustín Olmedo-Juárez; Tania Isabel Briones-Robles; Adrian Zaragoza-Bastida; Alejando Zamilpa; Deyanira Ojeda-Ramírez; Pedro Mendoza de Gives; Jaime Olivares-Pérez; Nallely Rivero-Perez
Source:
Microbial pathogenesis 2019 pp. -
ISSN:
0882-4010
Subject:
Caesalpinia coriaria; Escherichia coli; Listeria monocytogenes; Pseudomonas aeruginosa; Salmonella Typhi; Staphylococcus aureus; antibacterial properties; antibiotic resistance; bacteria; drugs; ethyl acetate; fruits; high performance liquid chromatography; in vitro studies; methyl gallate; minimum inhibitory concentration; nuclear magnetic resonance spectroscopy; public health; secondary metabolites
Abstract:
... Antimicrobial resistance has been increasing in recent years and is most frequently found in pathogenic microorganisms resistant or multiresistant to drugs. The secondary metabolites of plants have been evaluated as alternatives for control and treatment of these microorganisms. The aim of this study was to isolate and identify the secondary metabolites with antibacterial activity from Caesalpinia ...
DOI:
10.1016/j.micpath.2019.103660

94. Antibacterial activity of crab haemocyanin against clinical pathogens

Author:
Yoana Krasimirova Kizheva; Iliyana Kirilova Rasheva; Milena Nikolova Petrova; Anna Vasileva Milosheva-Ivanova; Lyudmila Georgieva Velkova; Pavlina Aleksandrova Dolashka; Aleksandar Konstantinov Dolashki; Petya Koicheva Hristova
Source:
Biotechnology, biotechnological equipment 2019 v.33 no.1 pp. 873-880
ISSN:
1314-3530
Subject:
Bacillus subtilis; Escherichia coli; antibacterial properties; antibiotic resistance; biotechnology; carbohydrate content; chromatography; crabs; equipment; glycosylation; hemocyanin; hemolymph
Abstract:
... The increasing antibiotic resistance among pathogenic bacteria is a challenge that drives the development of new antibacterial substances. Marine inhabitants are excellent sources of antimicrobial proteins and considered as promising candidates for the treatment of microbial infections. In the present study, we obtained haemocyanin from Eriphia verrucosa and studied its potential to suppress the g ...
DOI:
10.1080/13102818.2019.1626283

95. Antibacterial activity of major royal jelly proteins of the honeybee (Apis mellifera) royal jelly

Author:
Hee Geun Park; Bo Yeon Kim; Min Ji Park; Yijie Deng; Yong Soo Choi; Kwang Sik Lee; Byung Rae Jin
Source:
Journal of Asia-Pacific entomology 2019 v.22 no.3 pp. 737-741
ISSN:
1226-8615
Subject:
Apis mellifera; Escherichia coli; Gram-negative bacteria; antibacterial properties; cell walls; honey bees; proteins; royal jelly
Abstract:
... Major royal jelly proteins (MRJPs) are the protein components in royal jelly (RJ). MRJPs 1–7 are detected in the honeybee Apis mellifera RJ. Although A. mellifera MRJP (AmMRJP) 2 exhibited antibacterial activity, the other MRJPs with antimicrobial activities in A. mellifera RJ remains largely unknown. Here, we compared the antibacterial activity of recombinant AmMRJPs 1–7 expressed in baculovirus- ...
DOI:
10.1016/j.aspen.2019.06.005

96. Antibacterial activity of sustainable composites derived from epoxidized natural rubber/silver-substituted zeolite/poly(lactic acid) blends

Author:
Phruedsaporn Taranamai; Pranee Phinyocheep; Watanalai Panbangred; Mayura Janhom; Philippe Daniel
Source:
Journal of materials science 2019 v.54 no.14 pp. 10389-10409
ISSN:
0022-2461
Subject:
Escherichia coli; Staphylococcus aureus; antibacterial properties; differential scanning calorimetry; disk diffusion antimicrobial test; energy-dispersive X-ray analysis; hydrophilicity; impact strength; milling; polylactic acid; rubber; scanning electron microscopy; temperature; water uptake; zeolites
Abstract:
... Sustainable composites derived from epoxidized natural rubber (ENR)/silver-substituted zeolite (AgZ)/poly(lactic acid) (PLA) blends possessing antibacterial activity were reported. ENR, herein, acted as an antibacterial promoter providing more hydrophilicity to the composites and facilitating water diffusion. Two methodologies were used to prepare composites, including solution casting (S) as well ...
DOI:
10.1007/s10853-019-03599-1

97. Antibacterial and hydroxyapatite-forming coating for biomedical implants based on polypeptide-functionalized titania nanospikes

Author:
Qiang Gao; Tao Feng; Danni Huang; Peng Liu; Peng Lin; Yan Wu; Zhaoming Ye; Jian Ji; Peng Li; Wei Huang
Source:
Biomaterials science 2019 v.8 no.1 pp. 278-289
ISSN:
2047-4849
Subject:
Escherichia coli; Staphylococcus aureus; antibacterial properties; bacteria; bacterial adhesion; bacterial infections; biofilm; biomimetics; body fluids; coatings; hot water treatment; hydroxyapatite; mice; nanomaterials; orthopedics; osteoblasts; polypeptides; titanium; titanium dioxide; virulent strains
Abstract:
... Titanium (Ti)-based implants often suffer from detrimental bacterial adhesion and inefficient healing, so it is crucial to design a dual-functional coating that prevents bacterial infection and enhances bioactivity for a successful implant. Herein, we successfully devised a cationic polypeptide (Pep)-functionalized biomimetic nanostructure coating with superior activity, which could not only kill ...
DOI:
10.1039/c9bm01396b

98. Antibacterial effect of boron nitride flakes with controlled orientation in polymer composites

Author:
Santosh Pandit; Karolina Gaska; V. R. S. S. Mokkapati; Sven Forsberg; Magnus Svensson; Roland Kádár; Ivan Mijakovic
Source:
RSC advances 2019 v.9 no.57 pp. 33454-33459
ISSN:
2046-2069
Subject:
Escherichia coli; Pseudomonas aeruginosa; Staphylococcus aureus; Staphylococcus epidermidis; antibacterial properties; bacterial adhesion; boron nitride; coatings; extrusion; graphene; medical equipment; melting; nanocomposites; nanoparticles; polyethylene; scanning electron microscopy; viability assays
Abstract:
... Boron nitride (BN) is a stable 2D material with physiochemical properties similar to graphene-based nanomaterials. We have recently demonstrated that vertically aligned coatings of graphene-based nanomaterials provide strong antibacterial effects on various surfaces. Here we investigated whether BN, a nanomaterial with extensive similarities to graphene, might exhibit similar antibacterial propert ...
DOI:
10.1039/c9ra06773f

99. Antibacterial mechanism of Tetrastigma hemsleyanum Diels et Gilg's polysaccharides by metabolomics based on HPLC/MS

Author:
Xiao Chen; Lan Tao; Yi Ru; Shaohuang Weng; Zhou Chen; Jian Wang; Longhua Guo; Zhenyu Lin; Wei Pan; Bin Qiu
Source:
International journal of biological macromolecules 2019 v.140 pp. 206-215
ISSN:
0141-8130
Subject:
Escherichia coli; Tetrastigma; antibacterial properties; fructose 6-phosphate; gluconeogenesis; glycolysis; herbal medicines; high performance liquid chromatography; medicine; metabolomics; pharmacology; polysaccharides
Abstract:
... Tetrastigma hemsleyanum Diels et Gilg (THDG) is used as a Chinese traditional anti-inflammatory medicine for about thousands of years. In this work, Tetrastigma hemsleyanum Diels et Gilg's polysaccharide (TP) can inhibit E. coli's growth in initial dosing period. Compared with the antibacterial effect of Achyranthe's polysaccharide (AP) from their metabolic profile, it's obviously that their metab ...
DOI:
10.1016/j.ijbiomac.2019.08.097

100. Antibacterial potential of nudibranch-associated bacteria from Saparua and Nusa Laut Islands, Indonesia

Author:
RHESI KRISTIANA; MADA TRIANDALA SIBERO; MACELLYNE YOHANNA FARISA; DIAH AYUNINGRUM; DIO DIRGANTARA; MUHAMMAD HANAFI; OCKY KARNA RADJASA; AGUS SABDONO; AGUS TRIANTO
Source:
Biodiversitas 2019 v.20 no.7 pp. -
ISSN:
2085-4722
Subject:
Bacillus subtilis; Escherichia coli; Klebsiella pneumoniae; Micrococcus luteus; Pseudoalteromonas piscicida; Vibrio; Virgibacillus; antibacterial properties; beta-lactamase; ethyl acetate; genes; methicillin-resistant Staphylococcus aureus; minimum inhibitory concentration; multiple drug resistance; ribosomal RNA; Indonesia
Abstract:
... Kristiana R, Sibero MT, Farisa MY, Ayuningrum D, Dirgantara D, Hanafi M, Radjasa OK, Sabdono A, Trianto A. 2019. Antibacterial potential of nudibranch-associated bacteria from Saparua and Nusa Laut Islands, Indonesia. Biodiversitas 20: 1811-1819. Infections caused by multidrug-resistant bacteria are the international health issue that triggers the urgency of finding new antibacterial agents. The a ...
DOI:
10.13057/biodiv/d200704