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You searched for: Publication year rev 7981-2019 Remove constraint Publication year rev: 7981-2019 Subject term Humicola insolens Remove constraint Subject term: Humicola insolens
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1. Cutinases catalyze polyacrylate hydrolysis and prevent their aggregation

Author:
Ruoyu Hong; Lingqia Su; Jing Wu
Source:
Polymer degradation and stability 2019 v.159 pp. 23-30
ISSN:
0141-3910
Subject:
Fusarium solani; Humicola insolens; Thermobifida fusca; cutinase; dispersions; hydrolysis; pH; paper recycling; polyacrylic acid; porous media; recycled paper; temperature; turbidity; waste paper
Abstract:
... During the recycling of waste paper, the accumulation of polyacrylates present in the waste paper causes the formation of tacky substances known as stickies. Deposition of these stickies on the machinery decreases the quality of the recycled paper and increases the usage of circulating water. Enzymes that hydrolyze these polyacrylates can minimize or eliminate the deposition of stickies. In initia ...
DOI:
10.1016/j.polymdegradstab.2018.10.028

2. Enzymatic hydrolysis of poly(1,4-butylene 2,5-thiophenedicarboxylate) (PBTF) and poly(1,4-butylene 2,5-furandicarboxylate) (PBF) films: A comparison of mechanisms

Author:
Matteo Gigli; Felice Quartinello; Michelina Soccio; Alessandro Pellis; Nadia Lotti; Georg M. Guebitz; Silvia Licoccia; Andrea Munari
Source:
Environment international 2019 v.130 pp. 104852
ISSN:
0160-4120
Subject:
Humicola insolens; Thermobifida; chemical structure; cleavage (chemistry); cutinase; depolymerization; enzymatic hydrolysis; polyesters
Abstract:
... Enzymatic hydrolysis of poly(1,4-butylene 2,5-thiophenedicarboxylate) (PBTF) and poly(1,4-butylene 2,5-furandicarboxylate) (PBF) by Humicola insolens (HiC) and Thermobifida cellulosilytica (Cut) cutinases is investigated. For the first time, the different depolymerization mechanisms of PBTF (endo-wise scission) and PBF (exo-wise cleavage) has been unveiled and correlated to the chemical structure ...
DOI:
10.1016/j.envint.2019.05.046

3. High-fold improvement of assorted post-consumer poly(ethylene terephthalate) (PET) packages hydrolysis using Humicola insolens cutinase as a single biocatalyst

Author:
Aline Machado de Castro; Adriano Carniel; Diego Stahelin; Luiz Silvino Chinelatto Junior; Hercilio de Angeli Honorato; Sonia Maria Cabral de Menezes
Source:
Process biochemistry 2019 v.81 pp. 85-91
ISSN:
1359-5113
Subject:
Humicola insolens; biocatalysts; circular economy; cutinase; depolymerization; hydrolysis; plastics; polyethylene terephthalates; recycling
Abstract:
... The dissemination of technologies for poly(ethylene terephthalate) (PET) recycling is of paramount importance in the context of the plastics circular economy. One of the most promising alternatives is to use enzymes as catalysts for PET depolymerization to its monomers, but this route still needs improvement, especially regarding titer and productivity. In the present work, a sequential approach c ...
DOI:
10.1016/j.procbio.2019.03.006

4. High-level expression of Humicola insolens cutinase in Pichia pastoris without carbon starvation and its use in cotton fabric bioscouring

Author:
Ruoyu Hong; Yirong Sun; Lingqia Su; Lengtao Gu; Fuqing Wang; Jing Wu
Source:
Journal of biotechnology 2019 v.304 pp. 10-15
ISSN:
0168-1656
Subject:
Humicola insolens; Komagataella pastoris; Thermobifida fusca; bioreactors; bioscouring; butyrates; carbon; cell growth; cotton fabric; cutinase; enzyme activity; fermentation; glycerol; methanol; monitoring; sorbitol; starvation; wettability
Abstract:
... Huimcola insolens cutinase (HiC) was heterologously expressed in Pichia pastoris. To avoid a carbon starvation step, fermentation was conducted using combinations of sorbitol with glycerol and methanol in the cell growth and induction phases, respectively. The cutinase productivity (27.71 U mL−1 h−1) was 9.93 U mL−1 h−1 greater than that achieved using traditional two-phase methods, and a cutinase ...
DOI:
10.1016/j.jbiotec.2019.07.011

5. Immobilized feruloyl esterase from Humicola insolens catalyzes the synthesis of feruloylated oligosaccharides

Author:
Juan Tamayo-Cabezas; Salwa Karboune
Source:
Process biochemistry 2019 v.79 pp. 81-90
ISSN:
1359-5113
Subject:
Humicola insolens; acylation; catalytic activity; emulsions; epoxides; ferulic acid; feruloyl esterase; magnetism; microparticles; nanoparticles; raffinose; substrate specificity
Abstract:
... Ferulic acid acylation of oligosaccharides catalyzed by feruloyl esterases (FAE) is a promising route to produce feruloylated oligosaccharides. However, modulation of FAE synthetic properties is a key step to improve the acylation. The efficiency of H. insolens FAE to catalyze the feruloylation in six different surfactantless microemulsions reaction systems was evaluated. The highest yield (57%) w ...
DOI:
10.1016/j.procbio.2018.12.013

6. Polysaccharide monooxygenase-catalyzed oxidation of cellulose to glucuronic acid-containing cello-oligosaccharides

Author:
Jinyin Chen; Xiuna Guo; Min Zhu; Chen Chen; Duochuan Li
Source:
Biotechnology for biofuels 2019 v.12 no.1 pp. 42
ISSN:
1754-6834
Subject:
Humicola insolens; Komagataella pastoris; beta-glucosidase; beta-glucuronidase; catalytic activity; cellulose; genes; glucose; glucuronic acid; hydrolysis; moieties; oligosaccharides; oxidation
Abstract:
... BACKGROUND: Polysaccharide monooxygenases (PMOs) play an important role in the enzymatic degradation of cellulose. They have been demonstrated to able to C6-oxidize cellulose to produce C6-hexodialdoses. However, the biological function of C6 oxidation of PMOs remains unknown. In particular, it is unclear whether C6-hexodialdoses can be further oxidized to uronic acid (glucuronic acid-containing o ...
DOI:
10.1186/s13068-019-1384-0
PubMed:
30858879
PubMed Central:
PMC6391835

7. Switched reaction specificity in polyesterases towards amide bond hydrolysis by enzyme engineering

Author:
Antonino Biundo; Raditya Subagia; Michael Maurer; Doris Ribitsch; Per-Olof Syrén; Georg M. Guebitz
Source:
RSC advances 2019 v.9 no.62 pp. 36217-36226
ISSN:
2046-2069
Subject:
Humicola insolens; Thermobifida; amidase; amides; biocatalysts; catalytic activity; cutinase; depolymerization; engineering; enzyme activity; hydrogen bonding; hydrolysis; microplastics; molecular dynamics; nylon; polyamides; polyethylene terephthalates; recycling
Abstract:
... The recalcitrance of plastics like nylon and other polyamides contributes to environmental problems (e.g. microplastics in oceans) and restricts possibilities for recycling. The fact that hitherto discovered amidases (EC 3.5.1. and 3.5.2.) only show no, or low, activity on polyamides currently obstructs biotechnological-assisted depolymerization of man-made materials. In this work, we capitalized ...
DOI:
10.1039/c9ra07519d