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1. Purification and Characterization of Superoxide Dismutases from Sea Buckthorn and Chestnut Rose

Author:
Hou, Zhiqiang; Zhao, Liang; Wang, Yongtao; Liao, Xiaojun
Source:
Journal of food science 2019 v.84 no.4 pp. 746-753
ISSN:
0022-1147
Subject:
Rosa roxburghii,   etc   ; Castanea; Hippophae rhamnoides; ammonium sulfate; anion exchange chromatography; antioxidants; autoxidation; circular dichroism spectroscopy; electrophoresis; fluorescence emission spectroscopy; gastric juice; humans; hydrodynamics; isoelectric point; light scattering; molecular weight; pH; pyrogallol; superoxide dismutase; temperature; tryptophan; water solubility;     Show all 22 Subjects
Abstract:
... Superoxide dismutases (SODs) were purified from sea buckthorn and chestnut rose by ammonium sulfate precipitation and anion‐exchange chromatography, and the detection methods of water‐soluble tetrazolium‐1 (WST‐1), nitrobluetetrazolium (NBT) and pyrogallol autoxidation (PA) for SOD activity were compared. WST‐1 method was selected due to its coefficient of variation (CV) <6% in this study. Two SOD ...
DOI:
10.1111/1750-3841.14441