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You searched for: Publication year rev 7981-2019 Remove constraint Publication year rev: 7981-2019 Subject term biocatalysts Remove constraint Subject term: biocatalysts
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1. Pistacia terebinthus Resin as Yeast Immobilization Support for Alcoholic Fermentation

Author:
Michalis Kallis; Konstantinos Sideris; Nikolaos Kopsahelis; Loulouda Bosnea; Yiannis Kourkoutas; Antonia Terpou; Maria Kanellaki
Source:
Foods 2019 v.8 no.4 pp. -
ISSN:
2304-8158
Subject:
Pistacia terebinthus; Saccharomyces cerevisiae; alcoholic fermentation; aldehydes; ambient temperature; biocatalysts; bioprocessing; commercialization; esters; fermented beverages; gallic acid; gas chromatography-mass spectrometry; ketones; phenols; principal component analysis; spoilage; storage temperature; storage time; sugars; terpenoids; trees; yeasts
Abstract:
... A natural resin retrieved from Pistacia terebinthus tree was evaluated as an immobilization carrier of Saccharomyces cerevisiae AXAZ-1 cells targeting successive fermentation batches of sugar synthetic mediums. Fermentation times below 54 h were recorded at temperatures 28–14 °C. In total, 147 compounds were detected using gas chromatography-mass spectrometry (GC-MS) analysis, including ...
DOI:
10.3390/foods8040127
PubMed:
30999587
PubMed Central:
PMC6518291

2. Acetylacetone extends the working life of laccase in enzymatic transformation of malachite green by interfering with a key intermediate

Author:
Wenguang Huang; Hua Yang; Shujuan Zhang
Source:
Journal of hazardous materials 2019 v.366 pp. 520-528
ISSN:
0304-3894
Subject:
biocatalysts; laccase; malachite green; statistical models
Abstract:
... The potential of acetylacetone (AA) as a mediator of laccase has been tested in the enzymatic transformation of malachite green (MG). AA inhibited the laccase-induced transformation of MG at the beginning of incubation but extended the working life of laccase in long runs. To elucidate the underlying mechanisms, the transformation of MG in the laccase-AA system was systematically investigated. The ...
DOI:
10.1016/j.jhazmat.2018.12.028

3. Acoustic cavitation assisted lipase B catalysed synthesis of polyethylene glycol stearate in a solvent free system via esterification: synthesis and optimization

Author:
Girish S Nhivekar; Virendra K Rathod
Source:
Journal of chemical technology and biotechnology 2019 v.94 no.8 pp. 2465-2474
ISSN:
0268-2575
Subject:
Pseudozyma antarctica; acoustics; agitation; biocatalysts; carboxylic ester hydrolases; catalytic activity; esterification; immobilized enzymes; mixing; polyethylene glycol; sieves; solvents; stearic acid; temperature; ultrasonics
Abstract:
... BACKGROUND: The current work describes the application of ultrasound and its improving effect on the synthesis of polyethylene glycol (PEG) stearate using Fermase (Fermase CALB™10,000 Candida antarctica Lipase B) as a biocatalyst via esterification of PEG600 and stearic acid. The influence of various parameters like molar ratio, temperature, speed of agitation, enzyme loading, effect of addition o ...
DOI:
10.1002/jctb.6030

4. Advances in cloning, structural and bioremediation aspects of nitrile hydratases

Author:
K. Supreetha; Saroja Narsing Rao; D. Srividya; H. S. Anil; S. Kiran
Source:
Molecular biology reports 2019 v.46 no.4 pp. 4661-4673
ISSN:
0301-4851
Subject:
acrylamides; biocatalysts; bioinformatics; bioremediation; biotransformation; catalytic activity; crystal structure; enantioselectivity; gene expression; genes; industry; metabolism; metagenomics; microorganisms; nicotinamide; nitrile hydratase; nitriles; thermal stability; toxicity
Abstract:
... Nitrile hydratase (NHase) is a prominent enzyme in many microorganisms for its nitrile metabolism. The potentiality in the bioconversion of nitriles to its high-value amides has been extensively used in industries for the production of acrylamide and nicotinamide which are essential chemicals. Enzymologists are still considering NHases for its potential biotechnological applications including biot ...
DOI:
10.1007/s11033-019-04811-w

5. Agarose-based microwell array chip for high-throughput screening of functional microorganisms

Author:
Leicheng Zhang; Peng Chen; Zhuoyue Zhou; Yawei Hu; Qiuyue Sha; Houjin Zhang; Xin Liu; Wei Du; Xiaojun Feng; Bi-Feng Liu
Source:
Talanta 2019 v.191 pp. 342-349
ISSN:
0039-9140
Subject:
agarose; bacteria; bacterial communities; biocatalysts; carboxylic ester hydrolases; enzymatic reactions; fluorescence; fluorescent dyes; hydrolysis; irradiation; new species; phenotype; rhodamines; ribosomal RNA; screening; sequence analysis; synthetic biology; ultraviolet radiation
Abstract:
... Natural bacterial communities provide a rich source of biocatalysts, however, high-throughput screening for the functional bacteria remains a major challenge. Here, we present an agarose-based microwells array chip for functionally screening and isolating novel microorganisms with merits of high-throughput, high specificity and sensitivity. In this approach, the bacterial cells were loaded with si ...
DOI:
10.1016/j.talanta.2018.08.090

6. Agarose-chitosan hydrogel-immobilized horseradish peroxidase with sustainable bio-catalytic and dye degradation properties

Author:
Muhammad Bilal; Tahir Rasheed; Yuping Zhao; Hafiz M.N. Iqbal
Source:
International journal of biological macromolecules 2019 v.124 pp. 742-749
ISSN:
0141-8130
Subject:
biocatalysts; catalytic activity; dyes; functional properties; high performance liquid chromatography; hydrogels; oxidation; pH; peroxidase; scanning electron microscopy; temperature
Abstract:
... Herein, we developed and characterized robust agarose–chitosan hydrogel using N‑hydroxysuccinimide (NHS) as a mild chemical cross-linker. The hydrogel offered a simple, effective and eco-friendlier support material with >90% of immobilization efficiency of horseradish peroxidase. The surface morphology and functional properties of the agarose–chitosan hydrogel with and without immobilized horserad ...
DOI:
10.1016/j.ijbiomac.2018.11.220

7. Amination of ficin extract to improve its immobilization on glyoxyl-agarose: Improved stability and activity versus casein

Author:
El-Hocine Siar; Roberto Morellon-Sterling; Mohammed Nasreddine Zidoune; Roberto Fernandez-Lafuente
Source:
International journal of biological macromolecules 2019 v.133 pp. 412-419
ISSN:
0141-8130
Subject:
agarose; amination; biocatalysts; casein; enzyme activity; ethylenediamines; ficain; hydrolysis; pH; urea
Abstract:
... Ficin extract has been aminated using ethylenediamine and carbodiimide to transform all exposed carboxylic groups into amino groups, retaining around 80% of activity versus benzoyl-d,l-arginine p-nitroanilide hydrochloride (BANA) and 90% versus casein. This aminated enzyme was then immobilized on glyoxyl agarose beads. After optimization of the immobilization protocol (immobilization at pH 10 for ...
DOI:
10.1016/j.ijbiomac.2019.04.123

8. Aminobacter sp. MSH1 Mineralizes the Groundwater Micropollutant 2,6-Dichlorobenzamide through a Unique Chlorobenzoate Catabolic Pathway

Author:
Bart Raes; Benjamin Horemans; Daniel Rentsch; Jeroen T’Syen; Maarten G. K. Ghequire; René De Mot; Ruddy Wattiez; Hans-Peter E. Kohler; Dirk Springael
Source:
Environmental science & technology 2019 v.53 no.17 pp. 10146-10156
ISSN:
1520-5851
Subject:
Aminobacter; amidase; biocatalysts; bioremediation; carbon; catabolism; chlorine; dehalogenation; energy; groundwater; hydroxylation; nitrogen; pollutants; tricarboxylic acid cycle
Abstract:
... 2,6-Dichlorobenzamide (BAM) is a major groundwater micropollutant posing problems for drinking water treatment plants (DWTPs) that depend on groundwater intake. Aminobacter sp. MSH1 uses BAM as the sole source of carbon, nitrogen, and energy and is considered a prime biocatalyst for groundwater bioremediation in DWTPs. Its use in bioremediation requires knowledge of its BAM-catabolic pathway, whic ...
DOI:
10.1021/acs.est.9b02021

9. Anaerobic Conversion of Lignocellulose to Materials for Biofuel Production: Volatile Fatty Acids and Ethanol

Author:
M. A. Gladchenko; S. N. Gaydamaka; V. P. Murygina; S. D. Varfolomeev
Source:
Applied biochemistry and microbiology 2019 v.55 no.7 pp. 756-764
ISSN:
0003-6838
Subject:
Clostridium acetobutylicum; acetic acids; acid hydrolysis; bacteria; biocatalysts; biomass; butyric acid; chemical oxygen demand; depolymerization; ethanol; fuel production; glycerol; hydrolysates; lignin; lignocellulose; reducing sugars; sawdust; volatile fatty acids; wheat straw
Abstract:
... The processes of oxidative depolymerization and acidic hydrolysis have consistently been used for the pretreatment of lignocellulosic biomass (wheat straw, sawdust, and lignin), which makes it possible to obtain a high content of soluble organic compounds in the hydrolysate (44–94 g COD/L) and to enhance the concentration of reducing sugars from 1 to 36%. The introduction of Clostridium acetobutyl ...
DOI:
10.1134/S0003683819070020

10. Anti-selective direct asymmetric Mannich reaction catalyzed by protease

Author:
Yu-Jue Chen; Yang Xiang; Yan-Hong He; Zhi Guan
Source:
Tetrahedron letters 2019 v.60 no.15 pp. 1066-1071
ISSN:
0040-4039
Subject:
Streptomyces griseus; aldehydes; biocatalysts; chemical reactions; chemical structure; diastereoselectivity; enantioselectivity; proteinases
Abstract:
... The anti-selective direct asymmetric Mannich reaction of (hetero) aromatic aldehydes, 4-anisidine and O-protected hydroxyacetones for the synthesis of stereodefined anti-β-amino-α-hydroxycarbonyl compounds was developed. Protease type XIV from Streptomyces griseus (SGP) was used as a biocatalyst in 1,4-dioxane/phosphate buffer under mild reaction conditions. The excellent diastereoselectivities of ...
DOI:
10.1016/j.tetlet.2019.03.031

11. Application of Immobilized Enzymes in Food Industry

Author:
Ekaterina D. Yushkova; Elena A. Nazarova; Anna V. Matyuhina; Alina O. Noskova; Darya O. Shavronskaya; Vladimir V. Vinogradov; Natalia N. Skvortsova; Elena F. Krivoshapkina
Source:
Journal of agricultural and food chemistry 2019 v.67 no.42 pp. 11553-11567
ISSN:
1520-5118
Subject:
biocatalysts; biocompatibility; cost effectiveness; food industry; immobilized enzymes; thermal stability
Abstract:
... Enzymes are macromolecular biocatalysts, widely used in food industry. In applications, enzymes are often immobilized on inert and insoluble carriers, which increase their efficiency due to multiple reusability. The properties of immobilized enzymes depend on the immobilization method and the carrier type. The choice of the carrier usually concerns the biocompatibility, chemical and thermal stabil ...
DOI:
10.1021/acs.jafc.9b04385

12. Application of a Multienzymatic System from Natural Latex in Key Reactions for oil-Based Biorefineries

Author:
Daniel Alberto Sánchez; Susana Raquel Morcelle; María Elisa Fait; Gabriela Marta Tonetto; María Luján Ferreira
Source:
Fermentation 2019 v.5 no.1 pp. -
ISSN:
2311-5637
Subject:
Araujia; biocatalysts; biorefining; esterification; fruits; hydrolysis; latex; oleic acid; soybean oil; triacylglycerols
Abstract:
... Oil-based biorefineries play a crucial role in the production of key platform chemicals that can be generated via biotechnological processes instead of a petrochemical route. This work focuses on the latex of the fruit of Araujia sericifera, which can be considered a multienzymatic system with applications in key reactions in oil-based biorefineries. The latex of Araujia sericifera (ASL) was used ...
DOI:
10.3390/fermentation5010018

13. Assessment of Ready-to-Use Freeze-dried Immobilized Biocatalysts as Innovative Starter Cultures in Sourdough Bread Making

Author:
Ioanna Mantzourani; Antonia Terpou; Athanasios Alexopoulos; Eugenia Bezirtzoglou; Stavros Plessas
Source:
Foods 2019 v.8 no.1 pp. -
ISSN:
2304-8158
Subject:
Lactobacillus delbrueckii subsp. bulgaricus; Lactobacillus paracasei; acetic acid; acidity; biocatalysts; breadmaking; cheese whey; cryoprotectants; freeze drying; gas chromatography-mass spectrometry; lactic acid; microorganisms; sodium hydroxide; sourdough bread; spoilage; starter cultures; volatile compounds; wheat bran
Abstract:
... In the present study the effect of innovative biocatalysts as starter cultures in sourdough bread making was explored. The biocatalysts consisted of Lactobacillus paracasei K5 and Lactobacillus bulgaricus ATCC 11842 (in single and mixed form), immobilized on delignified wheat bran (DWB), and freeze dried without cryoprotectants. The parameters monitored were physicochemical characteristics, mold a ...
DOI:
10.3390/foods8010040
PubMed:
30669666
PubMed Central:
PMC6352066

14. Atypical organic-solvent tolerant bacterial hormone sensitive lipase-like homologue EstAG1 from Staphylococcus saprophyticus AG1: Synthesis and characterization

Author:
Alisa Gricajeva; Ingrida Bikutė; Lilija Kalėdienė
Source:
International journal of biological macromolecules 2019 v.130 pp. 253-265
ISSN:
0141-8130
Subject:
Escherichia coli; Staphylococcus saprophyticus; bacteria; biocatalysts; biodiesel; biotechnology; carboxylic ester hydrolases; dimethyl sulfoxide; dioxane; esters; ethanol; flavor; leadership; markets; models; pH; polluted soils; sequence homology; Lithuania
Abstract:
... Biocatalysts exerting activity against ester bonds have a broad range of applications in modern biotechnology. Some of the most industrially relevant enzymes of this type are lipolytic and their market is predicted to uphold leadership up till 2024. In this study, a novel bacterial hormone-sensitive lipase-like (bHSL) family homologue, designated EstAG1, was discovered by mining gDNA of bacteria i ...
DOI:
10.1016/j.ijbiomac.2019.02.110

15. Awakening the Sleeping Carboxylase Function of Enzymes: Engineering the Natural CO₂-Binding Potential of Reductases

Author:
Iria Bernhardsgrütter; Kristina Schell; Dominik M. Peter; Farshad Borjian; David Adrian Saez; Esteban Vöhringer-Martinez; Tobias J. Erb
Source:
Journal of the American Chemical Society 2019 v.141 no.25 pp. 9778-9782
ISSN:
1520-5126
Subject:
Erythrobacter; active sites; biocatalysts; bioinformatics; biotechnology; carbon dioxide; carboxylation; enzymatic reactions; enzymes; proteins; synthetic biology
Abstract:
... Developing new carbon dioxide (CO₂) fixing enzymes is a prerequisite to create new biocatalysts for diverse applications in chemistry, biotechnology and synthetic biology. Here we used bioinformatics to identify a “sleeping carboxylase function” in the superfamily of medium-chain dehydrogenases/reductases (MDR), i.e. enzymes that possess a low carboxylation side activity next to their original enz ...
DOI:
10.1021/jacs.9b03431
PubMed:
31188584
PubMed Central:
PMC6650136

16. Bacillus subtilis immobilization in alginate microfluidic-based microparticles aiming to improve lipase productivity

Author:
Aline F. Oliveira; Reinaldo G. Bastos; Lucimara G. de la Torre
Source:
Biochemical engineering journal 2019 v.143 pp. 110-120
ISSN:
1369-703X
Subject:
Bacillus subtilis; alginates; bacteria; biocatalysts; bioprocessing; carboxylic ester hydrolases; immobilized cells; microfluidic technology; microparticles; nutrient transport
Abstract:
... Biocatalyst immobilization is an important strategy to improve bioprocess productivity. However, conventional immobilization techniques produce non-uniform particles of millimetric size that may affect the cellular response by limiting nutrient transport into the particles. Droplet-based microfluidics can be an alternative to generate homogeneous and micrometric particles resulting in a reduced nu ...
DOI:
10.1016/j.bej.2018.12.014

17. Bacterial Respiratory Chain Diversity Reveals a Cytochrome c Oxidase Reducing O₂ at Low Overpotentials

Author:
Xie Wang; Romain Clément; Magali Roger; Marielle Bauzan; Ievgen Mazurenko; Anne de Poulpiquet; Marianne Ilbert; Elisabeth Lojou
Source:
Journal of the American Chemical Society 2019 v.141 no.28 pp. 11093-11102
ISSN:
1520-5126
Subject:
Acidithiobacillus ferrooxidans; bacteria; biocatalysts; carbon; carbon nanofibers; cytochrome c; cytochrome-c oxidase; electrochemistry; electrodes; electron transfer; electron transport chain; hydrophobicity; oxygen; pH
Abstract:
... Cytochrome c oxidases (CcOs) are the terminal enzymes in energy-converting chains of microorganisms, where they reduce oxygen into water. Their affinity for O₂ makes them attractive biocatalysts for technological devices in which O₂ concentration is limited, but the high overpotentials they display on electrodes severely limit their applicative use. Here, the CcO of the acidophilic bacterium Acidi ...
DOI:
10.1021/jacs.9b03268

18. Bacterial nitrilases and their regulation

Author:
Varsha P. Chhiba-Govindjee; Chris W. van der Westhuyzen; Moira L. Bode; Dean Brady
Source:
Applied microbiology and biotechnology 2019 v.103 no.12 pp. 4679-4692
ISSN:
0175-7598
Subject:
Streptomyces; amides; biocatalysts; biochemical pathways; carboxylic acids; drugs; environmental factors; heterocyclic compounds; multigene family; nitrilase; nitriles; transcription factors
Abstract:
... Commercially, nitrilases are valuable biocatalysts capable of converting a diverse range of nitriles to carboxylic acids for the greener synthesis of chemicals and pharmaceuticals. Nitrilases are widespread in nature and are both important components of metabolic pathways and a response to environmental factors such as natural or manmade nitriles. Nitrilases are often grouped together on a genome ...
DOI:
10.1007/s00253-019-09776-1

19. Batch and continuous cultivation processes of Candida tropicalis TISTR 5306 for ethanol and pyruvate decarboxylase production in fresh longan juice with optimal carbon to nitrogen molar ratio

Author:
Rojarej Nunta; Charin Techapun; Kittisak Jantanasakulwong; Thanongsak Chaiyaso; Phisit Seesuriyachan; Julaluk Khemacheewakul; Chatchadaporn Mahakuntha; Kritsadaporn Porninta; Sumeth Sommanee; Ngoc T. Trinh; Noppol Leksawasdi
Source:
Journal of food process engineering 2019 v.42 no.6 pp. e13227
ISSN:
0145-8876
Subject:
Candida tropicalis; ammonium sulfate; batch systems; biocatalysts; biofuels; carbon; case studies; ethanol; fructose; fruit growing; fruit juices; glucose; longans; malt; marketing; nitrogen; nose; pulp; pyruvate decarboxylase; solvents; sucrose; yeasts; zero wastes; Thailand
Abstract:
... The carbon to nitrogen (C/N) molar ratio of 21.88 ± 0.20 in fresh juice from C‐grade longan (FLJ‐C) supplemented with ammonium sulfate (AMS) was optimal for cultivation of Candida tropicalis TISTR 5306 producing ethanol concentration of 24.0 ± 1.1 g/L and specific pyruvate decarboxylase (PDC) activity of 0.138 ± 0.001 U/mg protein. The highest ethanol concentration in a 10 L continuous process (34 ...
DOI:
10.1111/jfpe.13227

20. Benzyl butyrate esterification mediated by immobilized lipases: Evaluation of batch and fed-batch reactors to overcome lipase-acid deactivation

Author:
Alessandra Cristina de Meneses; Amanda Gomes Almeida Sá; Lindomar Alberto Lerin; Marcos Lúcio Corazza; Pedro Henrique Hermes de Araújo; Claudia Sayer; Débora de Oliveira
Source:
Process biochemistry 2019 v.78 pp. 50-57
ISSN:
1359-5113
Subject:
Pseudozyma antarctica; benzyl alcohol; biocatalysts; butyric acid; carboxylic ester hydrolases; esterification; immobilized enzymes
Abstract:
... In this work, benzyl butyrate was synthesized for the first time via biotechnological esterification of benzyl alcohol and butyric acid in a solvent-free system (SFS). The synthesis was maximized in batch reactors, considering a low substrates molar ratio of 1:1. Novozym 435, a well-known commercial immobilized lipase (Candida antarctica fraction B - Cal B), was the best biocatalyst in the benzyl ...
DOI:
10.1016/j.procbio.2018.12.029

21. Benzyl propionate synthesis by fed-batch esterification using commercial immobilized and lyophilized Cal B lipase

Author:
Alessandra Cristina de Meneses; Lindomar Alberto Lerin; Pedro Henrique Hermes Araújo; Cláudia Sayer; Débora de Oliveira
Source:
Bioprocess and biosystems engineering 2019 v.42 no.10 pp. 1625-1634
ISSN:
1615-7591
Subject:
Pseudozyma antarctica; benzyl alcohol; biocatalysts; carboxylic ester hydrolases; esterification; freeze drying; light microscopy; propionic acid; protein content
Abstract:
... In this work, a fed-batch approach was adopted to overcome propionic acid lipase inactivation effects in the benzyl propionate direct esterification mediated by lipases. The ester synthesis was performed using commercial immobilized (Novozym 435) and lyophilized form Candida antarctica fraction B lipase (Cal B) as biocatalysts of the esterification between benzyl alcohol and propionic acid in a so ...
DOI:
10.1007/s00449-019-02159-w

22. Bilirubin oxidase-based silica macrocellular robust catalyst for on line dyes degradation

Author:
Armand Roucher; Elodie Roussarie; Régis M. Gauvin; Jad Rouhana; Sébastien Gounel; Claire Stines-Chaumeil; Nicolas Mano; Rénal Backov
Source:
Enzyme and microbial technology 2019 v.120 pp. 77-83
ISSN:
0141-0229
Subject:
Bacillus pumilus; ambient temperature; bilirubin; bilirubin oxidase; biocatalysts; catalytic activity; decolorization; dyes; laccase; pH; porosity; silica; silicon; storage temperature; wastewater treatment
Abstract:
... We present a new heterogeneous biocatalyst based on the grafting of Bilirubin Oxidase from Bacillus pumilus into macrocellular Si(HIPE) materials dedicated to water treatment. Due to the host intrinsic high porosity and monolithic character, on-line catalytic process is reached. We thus used this biocatalyst toward uni-axial flux decolorizations of Congo Red and Remazol Brilliant Blue (RBBR) at tw ...
DOI:
10.1016/j.enzmictec.2018.10.004

23. Bio-electrochemical power generation in petrochemical wastewater fed microbial fuel cell

Author:
Sumaya Sarmin; Baranitharan Ethiraj; M. Amirul Islam; Asmida Ideris; Chin Sim Yee; Md. Maksudur Rahman Khan
Source:
Science of the total environment 2019 v.695 pp. 133820
ISSN:
0048-9697
Subject:
Methanobacterium; Pseudomonas; acclimation; acrylic acid; biocatalysts; biofilm; chemical oxygen demand; dielectric spectroscopy; electrodes; electron transfer; energy; metabolites; methanogens; microbial fuel cells; microbial growth; power generation; sludge; voltammetry; wastewater
Abstract:
... The petrochemical wastewater (PCW) from acrylic acid plants possesses a very high chemical oxygen demand (COD) due to the presence of acrylic acid along with other organic acids. The treatment of PCW by conventional aerobic and anaerobic methods is energy intensive. Therefore, the treatment of PCW with concurrent power generation by employing microbial fuel cell (MFC) could be a potential alternat ...
DOI:
10.1016/j.scitotenv.2019.133820

24. Biocatalysis in ionic liquids for lignin valorization: Opportunities and recent developments

Author:
Joseph C. Stevens; Jian Shi
Source:
Biotechnology advances 2019 v.37 no.8 pp. 107418
ISSN:
0734-9750
Subject:
biocatalysis; biocatalysts; biomass; biorefining; cellulose; depolymerization; directed evolution; economic sustainability; engineering; enzyme activity; feedstocks; hemicellulose; immobilized enzymes; industry; ionic liquids; lignin; lignocellulose; molecular weight; petroleum; protein engineering; sugars; temperature; thermal stability
Abstract:
... Lignin holds tremendous potential as a renewable feedstock for upgrading to a number of high-value chemicals and products that are derived from the petroleum industry at present. Since lignin makes up a significant fraction of lignocellulosic biomass, co-utilization of lignin in addition to cellulose and hemicelluloses is vital to the economic viability of cellulosic biorefineries. The recalcitran ...
DOI:
10.1016/j.biotechadv.2019.107418
CHORUS:
10.1016/j.biotechadv.2019.107418

25. Biocatalyst engineering of Thermomyces Lanuginosus lipase adsorbed on hydrophobic supports: Modulation of enzyme properties for ethanolysis of oil in solvent-free systems

Author:
Erick Abreu Silveira; Sonia Moreno-Perez; Alessandra Basso; Simona Serban; Rita Pestana-Mamede; Paulo W. Tardioli; Cristiane S. Farinas; Natalia Castejon; Gloria Fernandez-Lorente; Javier Rocha-Martin; Jose M. Guisan
Source:
Journal of biotechnology 2019 v.289 pp. 126-134
ISSN:
0168-1656
Subject:
Thermomyces lanuginosus; adsorption; biocatalysts; carboxylic ester hydrolases; chemical bonding; engineering; esters; ethanolysis; hexane; hydrophobicity; mass transfer; porous media; sunflower oil
Abstract:
... Different immobilized biocatalysts of Thermomyces lanuginosus lipase (TLL) exhibited different properties for the ethanolysis of high oleic sunflower oil in solvent-free systems. TLL immobilized by interfacial adsorption on octadecyl (C-18) supports lost its 1,3-regioselectivity and produced more than 99% of ethyl esters. This reaction was influenced by mass-transfer limitations. TLL adsorbed on m ...
DOI:
10.1016/j.jbiotec.2018.11.014

26. Biocatalytic Oxidation of Biobased Furan Aldehydes: Comparison of Toxicity and Inhibition of Furans toward a Whole-Cell Biocatalyst

Author:
Ai-Di Cheng; Sai-Sai Shi; Yao Li; Min-Hua Zong; Ning Li
Source:
ACS sustainable chemistry & engineering 2019 v.8 no.3 pp. 1437-1444
ISSN:
2168-0485
Subject:
Escherichia coli; biocatalysts; carboxylic acids; catalytic activity; engineering; enzymes; furfural; hydrophobicity; hydroxymethylfurfural; neutralization; oxidation; pH; pharmaceutical industry; polymers; space and time; toxicity; viability
Abstract:
... Furan carboxylic acids are promising and renewable building blocks in polymer and pharmaceutical industries. In this work, biocatalytic synthesis of furan carboxylic acids was performed from biobased furan aldehydes using recombinant Escherichia coli expressing 3-succinoylsemialdehyde-pyridine dehydrogenase (SAPDH). Particularly, the inhibition and toxicity of furan aldehydes and their carboxylic ...
DOI:
10.1021/acssuschemeng.9b05621

27. Biocatalytic esterification of fatty acids using a low-cost fermented solid from solid-state fermentation with Yarrowia lipolytica

Author:
Jéssica Rocha da Silva; Carlos Eduardo Conceição de Souza; Erika Valoni; Aline Machado de Castro; Maria Alice Zarur Coelho; Bernardo Dias Ribeiro; Cristiane Assumpção Henriques; Marta Antunes Pereira Langone
Source:
3 Biotech 2019 v.9 no.2 pp. 38
ISSN:
2190-572X
Subject:
Yarrowia lipolytica; biocatalysts; biodiesel; bran; carboxylic ester hydrolases; enzyme activity; esterification; ethanol; free fatty acids; freeze drying; fuel production; hexane; linoleic acid; oils; oleic acid; palmitic acid; raw materials; solid state fermentation; solvents; soybeans; stearic acid; washing; yeasts
Abstract:
... This study aimed to evaluate the use of a lyophilized fermented solid (named solid enzymatic preparation, SEP), with lipase activity, as a low-cost biocatalyst for esterification reactions of fatty acids present in acid raw materials for biodiesel synthesis. The SEP was obtained by solid-state fermentation (SSF) of soybean bran using the strain of Yarrowia lipolytica IMUFRJ 50682 and contains the ...
DOI:
10.1007/s13205-018-1550-2
PubMed:
30627506
PubMed Central:
PMC6323043

28. Biocatalytic membrane reactor development for organophosphates degradation

Author:
G. Vitola; R. Mazzei; T. Poerio; E. Porzio; G. Manco; I. Perrotta; F. Militano; L. Giorno
Source:
Journal of hazardous materials 2019 v.365 pp. 789-795
ISSN:
0304-3894
Subject:
Sulfolobus; artificial membranes; batch systems; biocatalysts; chemical bonding; extremophiles; human health; hydrophilicity; mutants; nerve agents; paraoxon; phosphoric triester hydrolases; solubilization; thermal stability; thermostable enzymes
Abstract:
... Organophosphates (OPs) are highly toxic compounds used as pesticides and nerve agents. The devastating effects, reported in different studies, on the environment and human health indicate a serious scenario for both instantaneous and long terms effects. Bio-based strategies for OPs degradation seem the most promising solutions, particularly when extremophiles enzymes are used. These systems permit ...
DOI:
10.1016/j.jhazmat.2018.11.063

29. Biocatalytic oxidation of flavone analogues mediated by general biocatalysts: horseradish peroxidase and laccase

Author:
Qingsong Yan; Xiaoguang Tang; Baojing Zhang; Chunjie Wang; Sa Deng; Xiaochi Ma; Chao Wang; Dawei Li; Shanshan Huang; Peipei Dong
Source:
RSC advances 2019 v.9 no.23 pp. 13325-13331
ISSN:
2046-2069
Subject:
biocatalysts; biosynthesis; chalcones; computer simulation; flavonols; heme; isoflavones; kinetics; laccase; oxidation; peroxidase; reaction mechanisms
Abstract:
... Horseradish peroxidase (HRP) and laccase are well known oxidases, which have been widely applied for the biosynthesis of organic compounds. In the present work, flavone analogues as an important type of bioactive natural product could be oxidized by HRP or laccase, which afforded dimeric and oxidative flavones. All of the flavone analogues usually possessing phenolic groups could be transformed us ...
DOI:
10.1039/c9ra00470j

30. Biocatalytic synthesis of panthenyl monoacyl esters in ionic liquids and deep eutectic solvents

Author:
Pedro Lozano; Elena Alvarez; Susana Nieto; Rocio Villa; Juana M. Bernal; Antonio Donaire
Source:
Green chemistry 2019 v.21 no.12 pp. 3353-3361
ISSN:
1463-9270
Subject:
biocatalysts; cations; dodecanoic acid; enzyme activity; esterification; esters; free fatty acids; green chemistry; ionic liquids; linoleic acid; myristic acid; oleic acid; palmitic acid
Abstract:
... The enzymatic synthesis of six panthenyl monoacyl esters (PMEs) was carried out by the direct esterification of fatty acids (i.e. capric, lauric, myristic, palmitic, oleic and linoleic acids, respectively) with panthenol in different ionic liquids (ILs) based on cations with a long alkyl side-chain (e.g. 1-dodecyl-3-methylimidazolium tetrafluoroborate [C₁₂mim][BF₄], etc.). All the assayed ILs were ...
DOI:
10.1039/c9gc01076a

31. Biochemical Characterization of Heat-Tolerant Recombinant L-Arabinose Isomerase from Enterococcus faecium DBFIQ E36 Strain with Feasible Applications in D-Tagatose Production

Author:
Ricardo Martín Manzo; André Saraiva Leão Marcelo Antunes; Jocélia de Sousa Mendes; Denise Cavalcante Hissa; Luciana Rocha Barros Gonҫalves; Enrique José Mammarella
Source:
Molecular biotechnology 2019 v.61 no.6 pp. 385-399
ISSN:
1073-6085
Subject:
Enterococcus faecium; Escherichia coli; GRAS substances; active ingredients; affinity chromatography; arabinose; bacteria; biocatalysts; biosynthesis; biotransformation; detergents; drug formulations; gene overexpression; genes; half life; heat tolerance; industry; isomerases; manganese; pH; sweetness; tagatose; taste sensitivity; virulence
Abstract:
... D-Tagatose is a ketohexose, which presents unique properties as a low-calorie functional sweetener possessing a sweet flavor profile similar to D-sucrose and having no aftertaste. Considered a generally recognized as safe (GRAS) substance by FAO/WHO, D-tagatose can be used as an intermediate for the synthesis of other optically active compounds as well as an additive in detergent, cosmetic, and ph ...
DOI:
10.1007/s12033-019-00161-x

32. Biochemical and structural characterization of a highly active branched-chain amino acid aminotransferase from Pseudomonas sp. for efficient biosynthesis of chiral amino acids

Author:
Xinxin Zheng; Yinglu Cui; Tao Li; Ruifeng Li; Lu Guo; Defeng Li; Bian Wu
Source:
Applied microbiology and biotechnology 2019 v.103 no.19 pp. 8051-8062
ISSN:
0175-7598
Subject:
Escherichia coli; Pseudomonas; alpha-ketoglutaric acid; amines; biocatalysts; biosynthesis; branched chain amino acids; catalytic activity; crystal structure; enantiomers; enantioselectivity; functional properties; glutamic acid; histidine; industrial applications; leucine; lysine; ornithine-oxo-acid transaminase; pH; regioselectivity; stereoselective synthesis; substrate specificity; temperature; threonine; valine
Abstract:
... Aminotransferases (ATs) are important biocatalysts for the synthesis of chiral amines because of their capability of introducing amino group into ketones or keto acids as well as their high enantioselectivity, high regioselectivity. Among all ATs, branched-chain amino acid aminotransferase (BCAT) can use branched-chain amino acids (BCAAs) as substrate, including L-valine, L-leucine, and L-isoleuci ...
DOI:
10.1007/s00253-019-10105-9

33. Biochemical and structural insights into PLP fold type IV transaminase from Thermobaculum terrenum

Author:
Ekaterina Yu. Bezsudnova; Konstantin M. Boyko; Alena Yu. Nikolaeva; Yulia S. Zeifman; Tatiana V. Rakitina; Dmitry A. Suplatov; Vladimir O. Popov
Source:
Biochimie 2019 v.158 pp. 130-138
ISSN:
0300-9084
Subject:
X-ray diffraction; active sites; amines; amino acids; bacteria; biocatalysis; biocatalysts; bioinformatics; chemical bonding; databases; enantioselectivity; enzyme kinetics; molecular models; pyridoxal; pyridoxal phosphate; pyridoxamine; substrate specificity; thermal stability; thermostable enzymes; transaminases
Abstract:
... The high catalytic efficiency of enzymes under reaction conditions is one of the main goals in biocatalysis. Despite the dramatic progress in the development of more efficient biocatalysts by protein design, the search for natural enzymes with useful properties remains a promising strategy. The pyridoxal 5'-phosphate (PLP)-dependent transaminases represent a group of industrially important enzymes ...
DOI:
10.1016/j.biochi.2018.12.017

34. Biochemical characterization and biocatalytic application of a novel d-tagatose 3-epimerase from Sinorhizobium sp.

Author:
Zhangliang Zhu; Chao Li; Xin Liu; Dengke Gao; Xueyu Wang; Masaru Tanokura; Hui-Min Qin; Fuping Lu
Source:
RSC advances 2019 v.9 no.6 pp. 2919-2927
ISSN:
2046-2069
Subject:
Sinorhizobium; active sites; amino acids; biocatalysts; biotransformation; catalytic activity; enzymes; fructose; hydrogen bonding; manganese; models; pH; psicose; sorbose; tagatose; temperature
Abstract:
... Sinorhizobium sp. d-tagatose 3-epimerase (sDTE) catalyzes the conversion of d-tagatose to d-sorbose. It also recognizes d-fructose as a substrate for d-allulose production. The optimal temperature and pH of the purified sDTE was 50 °C and 8.0, respectively. Based on the sDTE homologous model, Glu154, Asp187, Gln213, and Glu248, form a hydrogen bond network with the active-site Mn²⁺ and constitute ...
DOI:
10.1039/c8ra10029b

35. Biochemical characterization of a novel azoreductase from Streptomyces sp.: Application in eco-friendly decolorization of azo dye wastewater

Author:
Hao Dong; Tianyuan Guo; Wenxue Zhang; Hanjie Ying; Ping Wang; Yibing Wang; Yong Chen
Source:
International journal of biological macromolecules 2019 v.140 pp. 1037-1046
ISSN:
0141-8130
Subject:
Bacillus subtilis; NADH or NADPH oxidoreductases; Streptomyces; azo dyes; biocatalysts; bioremediation; decolorization; glucose 1-dehydrogenase (FAD, quinone); human health; industrial wastewater treatment; pH; pollution; solvents; wastewater
Abstract:
... Azo dyes are the most widely applied chemical dyes that have also raised great concerns for environmental contamination and human health issues. There has been a growing interest in discovering bioremediation methods to degrade azo dyes for environmental and economic purposes. Azoreductases are key enzymes evolved in nature capable of degrading azo dyes. The current work reports the identification ...
DOI:
10.1016/j.ijbiomac.2019.08.196

36. Biodesulfurization of diesel oil in oil–water two phase reaction system by Gordonia sp. SC-10

Author:
Shuiquan Chen; Chaocheng Zhao; Qiyou Liu; Xiuxia Zhang; Shuo Sun; Meng Zang
Source:
Biotechnology letters 2019 v.41 no.4-5 pp. 547-554
ISSN:
0141-5492
Subject:
bacteria; biocatalysts; desulfurization; diesel fuel; industrialization; organic sulfur compounds; sulfur
Abstract:
... OBJECTIVES: Different sulfur contents of diesel oils were used for biodesulfurization to study the desulfurization capacity of Gordonia sp. SC-10 in oil–water two-phase reaction system. RESULTS: Gordonia sp. SC-10 showed great properties in desulfurizing diesel oil with different sulfur contents. This bacterium could decrease sulfur contents in different diesel oils from 194.7 ± 3.7 to 30.4 ± 0.5  ...
DOI:
10.1007/s10529-019-02663-9

37. Biodiesel production using lipase immobilised functionalized magnetic nanocatalyst from oleaginous fungal lipid

Author:
Ranjitha Jambulingam; Madonna Shalma; Vijayalakshmi Shankar
Source:
Journal of cleaner production 2019 v.215 pp. 245-258
ISSN:
0959-6526
Subject:
Aspergillus niger; biocatalysts; biodiesel; enzyme activity; fatty acid composition; fatty acid methyl esters; fuel production; fungi; gas chromatography; immobilized enzymes; lipid content; lipids; magnetism; nanoparticles; oils; polluted soils; society; tannery effluents; triacylglycerol lipase
Abstract:
... From the tannery effluent and oil contaminated soil different microbe were isolated and screened for high lipid content. Among all the isolates fungal strain SF2 showed high lipid content was selected and identified as Aspergillus niger. It was found that the fungi produced about 44.02% of lipid. The fatty acid composition was identified by gas chromatography. The physicochemical property of the e ...
DOI:
10.1016/j.jclepro.2018.12.146

38. Bioelectricity generation using sulphate-reducing bacteria as anodic and microalgae as cathodic biocatalysts

Author:
Smita S. Kumar; Suddhasatwa Basu; Saloni Gupta; Jyoti Sharma; Narsi R. Bishnoi
Source:
Biofuels 2019 v.10 no.1 pp. 81-86
ISSN:
1759-7277
Subject:
Fourier transform infrared spectroscopy; aeration; algae culture; biocatalysts; bioelectricity; biomass; cathodes; lipid content; microalgae; microbial fuel cells; mixed culture; oxygen; photosynthesis; sulfate-reducing bacteria
Abstract:
... Because microalgal species carry out oxygenic photosynthesis, they can be employed in the cathode chamber of microbial fuel cells, thus negating the need for mechanical aeration for oxygen reduction reactions. A conventional H-shaped configuration is used for comparative analysis of bioelectricity generation using pure and mixed microalgal cultures. Three reactors are simultaneously inoculated wit ...
DOI:
10.1080/17597269.2018.1426161

39. Bioethanol Production From Turkish Hazelnut Husk Process Design and Economic Evaluation

Author:
Nihat Alpagu Sayar; Orkun Pinar; Dilek Kazan; Ahmet Alp Sayar
Source:
Waste and biomass valorization 2019 v.10 no.4 pp. 909-923
ISSN:
1877-2641
Subject:
Corylus colurna; agricultural wastes; biocatalysts; bioethanol; bioprocessing; case studies; economic evaluation; economic incentives; economic investment; environmental impact; ethanol; ethanol production; evaporation; hazelnuts; hulls; lignocellulose; nitrogen; operating costs; prices; process design; risk factors; sales; uncertainty analysis; Turkey (country)
Abstract:
... The main objective of the current study is to develop and assess the preliminary synthetic design steps of an innovative and unprecedented bioprocess plant converting Turkish hazelnut husk into lignocellulosic ethanol with an emphasis on economic evaluation. Valorization of this agricultural waste would provide a promising economic potential and long-term sustainability with acceptable environment ...
DOI:
10.1007/s12649-017-0103-y

40. Biofilm Formation by Monocultures and Mixed Cultures of Alcaligenes faecalis 2 and Rhodococcus ruber gt 1

Author:
A. S. Zorina; Yu. G. Maksimova; V. A. Demakov
Source:
Microbiology 2019 v.88 no.2 pp. 164-171
ISSN:
0026-2617
Subject:
Alcaligenes faecalis; Rhodococcus ruber; acetonitrile; acrylic acid; bacteria; biocatalysts; biofilm; biofilters; carbon; energy; enzyme activity; fluorescence; gentian violet; glucose; liquids; mixed culture; nitrile hydratase; plankton; polymers; quantitative analysis; staining; toxicity; wastewater
Abstract:
... Biofilm formation by monocultures and mixed cultures of nitrile-hydrolyzing bacteria Alcaligenes faecalis 2 and Rhodococcus ruber gt 1 was studied. Biofilm formation was assessed by staining with Crystal violet, by the energy state of the cells, and by the output of the polymer matrix. A quantitative method for assessment of the output of the polymer matrix by the fluorescence of konA-tetramethylr ...
DOI:
10.1134/S0026261719020140

41. Bioflavour Conference 2018—Biotechnology for Flavors, Fragrances, and Functional Ingredients

Author:
Jens Schrader; Holger Zorn; Christian von Wallbrunn
Source:
Journal of agricultural and food chemistry 2019 v.67 no.49 pp. 13363-13366
ISSN:
1520-5118
Subject:
bioactive compounds; biocatalysts; bioprocessing; biosynthesis; biotechnology; enzymes; flavor; functional foods; industry; odors; public speaking; receptors; wines; Germany
Abstract:
... The “Bioflavour 2018—Biotechnology of Flavors, Fragrances, and Functional Ingredients” conference was held from September 18th to 21st, 2018 at the DECHEMA house in Frankfurt, Germany. The conference attracted more than 190 participants from over 25 countries, with about 40% share from industry. Particular sessions of Bioflavour 2018 focused on “flavor perception and biotechnology”, “microbial cel ...
DOI:
10.1021/acs.jafc.9b05192

42. Biogas upgrading and biochemical production from gas fermentation: Impact of microbial community and gas composition

Author:
Basma Omar; Maie El-Gammal; Reda Abou-Shanab; Ioannis A. Fotidis; Irini Angelidaki; Yifeng Zhang
Source:
Bioresource technology 2019 v.286 pp. 121413
ISSN:
0960-8524
Subject:
Archaea; Moorella; acetates; acetogens; biocatalysts; biogas; carbon dioxide; energy; fermentation; heat treatment; hydrogen; inoculum; methanogens; microbial communities; mixed culture; ribosomal RNA; volatile fatty acids
Abstract:
... The present study proposes a novel alternative method of the current biogas upgrading techniques by converting CO2 (in the biogas) into valuable chemicals (e.g., volatile fatty acids) using H2 as energy source and acetogenic mixed culture as biocatalyst. The influence of thermal treatment (90 °C) on the inhibition of the methanogenic archaea and enriching the acetogenic bacteria in different inocu ...
DOI:
10.1016/j.biortech.2019.121413

43. Bioinspired genetic engineering of supramolecular assembled formate dehydrogenase with enhanced biocatalysis activities

Author:
Wei Jiang; Ruonan Yang; Peng Lin; Wenjing Hong; Baishan Fang
Source:
Journal of biotechnology 2019 v.292 pp. 50-56
ISSN:
0168-1656
Subject:
NAD (coenzyme); biocatalysis; biocatalysts; formate dehydrogenase; genetic engineering; proteins; scanning electron microscopy
Abstract:
... A bioinspired strategy for the synthesis of supramolecular and biocatalytical materials was developed base on protein-protein supramolecular interaction and genetic engineering. Formate dehydrogenase (FDH) and its functional fragments were separately fused to form a multi-function domain. The fusion proteins and functional fragments self-assembled into the expanded and controllable supramolecular ...
DOI:
10.1016/j.jbiotec.2018.12.017

44. Bioinspired synthesis of nanofibers on monolithic scaffolds for enzyme immobilization with enhanced loading capacity and activity recovery

Author:
Weiran Li; Jiafu Shi; Yang Zhao; Yu Tian; Qian Huo; Yizhou Wu; Shaohua Zhang; Zhongyi Jiang
Source:
Journal of chemical technology and biotechnology 2019 v.94 no.12 pp. 3763-3771
ISSN:
0268-2575
Subject:
active sites; biocatalysts; cordierite; electrostatic interactions; immobilized enzymes; mass transfer; mixing; nanofibers; peroxidase; polyethyleneimine; silica; sulfonates; wastewater treatment
Abstract:
... BACKGROUND: The enzymatic process in continuous flow reactors has become attractive owing to the merits of in‐line purification, improved mixing and mass transfer, etc. However, the construction of continuous flow reactors requires stable and surface‐active monolithic scaffolds for enzyme immobilization. RESULTS: In this study, nanofibers of linear polyethyleneimine (LPEI) are formed on the surfac ...
DOI:
10.1002/jctb.5963

45. Biological conversion of propane to 2-propanol using group I and II methanotrophs as biocatalysts

Author:
Thu Thi Nguyen; In Yeub Hwang; Jeong Geol Na; Eun Yeol Lee
Source:
Journal of industrial microbiology & biotechnology 2019 v.46 no.5 pp. 675-685
ISSN:
1367-5435
Subject:
1-propanol; EDTA (chelating agent); Methylobacter alcaliphilus; Methylomonas; Methylosinus trichosporium; acetone; additives; alcohol dehydrogenase; aldehydes; biocatalysis; biocatalysts; isopropyl alcohol; liquid petroleum gas; methane monooxygenases; methanol; methanotrophs; oxidation; petroleum; propane; sodium formate; sodium phosphate
Abstract:
... Propane is the main component of liquefied petroleum gas and is derived from crude oil processing. Methanotrophic bacteria can convert various alkanes using methane monooxygenase enzyme to primary alcohols. These are further oxidized to various aldehydes by alcohol dehydrogenases or methanol dehydrogenases. In this study, 2-propanol was produced from propane using the whole cells of Methylosinus t ...
DOI:
10.1007/s10295-019-02141-1

46. Biomass-bound biocatalysts for biodiesel production: Tuning a lipolytic activity from Aspergillus niger MYA 135 by submerged fermentation using agro-industrial raw materials and waste products

Author:
Erika Lucía Regner; Hebe Natalia Salvatierra; Mario Domingo Baigorí; Licia María Pera
Source:
Biomass and bioenergy 2019 v.120 pp. 59-67
ISSN:
0961-9534
Subject:
Aspergillus niger; alginates; biocatalysts; biodegradation; biodiesel; biomass; biotechnology; butanol; calcium chloride; carboxylic ester hydrolases; cooking fats and oils; corn meal; culture media; enzyme activity; ferric chloride; fuel production; kitchen waste; lipolysis; molasses; peptones; pollution control; raw materials; soybean oil; submerged fermentation; vinasse; whey
Abstract:
... The enzymatic route is a promising strategy to synthesize biodiesel essentially because of its moderate reaction conditions and easy product separation. Additionally, coupling the biodegradation of agro-industrial raw materials and waste products with the enzyme production has also had a significant impact on the development of industrial biotechnology. This is mainly due to the low-cost and the p ...
DOI:
10.1016/j.biombioe.2018.11.005

47. Bioorganic synthesis, characterization and evaluation of a natural phenolic lipid

Author:
Juliya Johny; Venkateshwarlu Kontham; Dileep Veeragoni; Sunil Misra; Shiva Shanker Kaki
Source:
Biotechnology reports 2019 v.24 pp. e00375
ISSN:
2215-017X
Subject:
2,2-diphenyl-1-picrylhydrazyl; Pseudozyma antarctica; antimicrobial properties; antioxidant activity; antioxidants; biocatalysts; carboxylic ester hydrolases; cell lines; cytotoxicity; differential scanning calorimetry; ferulic acid; glycerol; immobilized enzymes; inhibitory concentration 50; lipids; neoplasm cells; neoplasms; oxidation; soybean oil; spectral analysis
Abstract:
... The first synthesis of a phenolic natural monoacylglycerol (1- [11-(ferulyloxy) undecanoyl)] glycerol) was carried out by bioorganic synthesis starting from ferulic acid. The synthetic route of the target lipidic compound was designed involving a chemo-enzymatic approach using immobilized Candida antarctica lipase as biocatalyst in two of the steps conducted in organic medium. The prepared lipidic ...
DOI:
10.1016/j.btre.2019.e00375
PubMed:
31641619
PubMed Central:
PMC6796520

48. Bioprocessing of agro‐industrial residues into lactic acid and probiotic enriched livestock feed

Author:
Dragana Mladenović; Aleksandra Djukić‐Vuković; Miroslav Stanković; Marija Milašinović‐Šeremešić; Milica Radosavljević; Jelena Pejin; Ljiljana Mojović
Source:
Journal of the science of food and agriculture 2019 v.99 no.12 pp. 5293-5302
ISSN:
0022-5142
Subject:
Lactobacillus paracasei; agricultural industry; antimicrobial properties; antioxidants; batch fermentation; biocatalysts; bioprocessing; biorefining; circular economy; digestive tract; energy; environmental protection; food security; in vitro digestibility; lactic acid; livestock feeds; molasses; potatoes; probiotics; profitability; proteins; raw materials; ruminants; spent grains; sugar beet pulp; sunflower seed; value added; value-added products
Abstract:
... BACKGROUND: Growing challenges of resource depletion, food security and environmental protection are putting stress on the development of biorefinery processes for bioprocessing of residues from food and agro‐industry into value‐added products. In this study, the simultaneous production of lactic acid (LA) and livestock feed on a combined substrate based on molasses and potato stillage by Lactobac ...
DOI:
10.1002/jsfa.9759

49. Bioprospecting of whole-cell biocatalysts for cholesterol biotransformation

Author:
Victoria Giorgi; Michel Chaves; Pilar Menéndez; Carlos García Carnelli
Source:
World journal of microbiology & biotechnology 2019 v.35 no.1 pp. 12
ISSN:
0959-3993
Subject:
Acinetobacter; Aspergillus; Citrobacter; Exiguobacterium; Fusarium; Klebsiella; Mucor circinelloides; Proteus; Streptomyces; Talaromyces; Trichoderma; Tsukamurella; bacteria; biocatalysts; biotransformation; cholesterol; effluents; fungi; mass spectrometry; phylogeny; soil; value-added products; wastewater treatment; wool
Abstract:
... Microorganisms were isolated from industrial wool scouring effluents and from the soil adjacent to the wastewater treatment lagoon, both sterols-rich environments, in order to search for novel biocatalysts able to transform cholesterol. The isolates were identified on the basis of morphological and biochemical characteristics and phylogenetic analysis. Furthermore, a rapid and accurate bacteria id ...
DOI:
10.1007/s11274-018-2586-5

50. Bioprospection of Enzymes and Microorganisms in Insects to Improve Second-Generation Ethanol Production

Author:
Sérgio L. Alves; Caroline Müller; Charline Bonatto; Thamarys Scapini; Aline F. Camargo; Gislaine Fongaro; Helen Treichel
Source:
Industrial biotechnology 2019 v.15 no.6 pp. 336-349
ISSN:
1931-8421
Subject:
alcoholic fermentation; biocatalysts; biomass; cellobiose; cellulose; economic impact; enzymes; ethanol; ethanol production; food safety; fossil fuels; greenhouse gas emissions; hemicellulose; hexoses; hydrolysis; lignocellulose; microbiome; pectins; pentoses; phytophagous insects; xylose; yeasts
Abstract:
... The search for increased efficiency and lower costs for second-generation (2G) bioethanol production is generated by concerns regarding food safety and crop expansion, because the first generation of this fuel competes with production of food for consumption. There is also interest in decreasing associated greenhouse gas emissions and dependence on fossil fuels. However, processing 2G ethanol is e ...
DOI:
10.1089/ind.2019.0019