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You searched for: Publication year rev 7981-2019 Remove constraint Publication year rev: 7981-2019 Subject term biocatalysts Remove constraint Subject term: biocatalysts
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1. Pistacia terebinthus Resin as Yeast Immobilization Support for Alcoholic Fermentation

Author:
Michalis Kallis; Konstantinos Sideris; Nikolaos Kopsahelis; Loulouda Bosnea; Yiannis Kourkoutas; Antonia Terpou; Maria Kanellaki
Source:
Foods 2019 v.8 no.4 pp. -
ISSN:
2304-8158
Subject:
Pistacia terebinthus; Saccharomyces cerevisiae; alcoholic fermentation; aldehydes; ambient temperature; biocatalysts; bioprocessing; commercialization; esters; fermented beverages; gallic acid; gas chromatography-mass spectrometry; ketones; phenols; principal component analysis; spoilage; storage temperature; storage time; sugars; terpenoids; trees; yeasts
Abstract:
... A natural resin retrieved from Pistacia terebinthus tree was evaluated as an immobilization carrier of Saccharomyces cerevisiae AXAZ-1 cells targeting successive fermentation batches of sugar synthetic mediums. Fermentation times below 54 h were recorded at temperatures 28–14 °C. In total, 147 compounds were detected using gas chromatography-mass spectrometry (GC-MS) analysis, including ...
DOI:
10.3390/foods8040127
PubMed:
30999587
PubMed Central:
PMC6518291

2. A DNAzyme-powered cross-catalytic circuit for amplified intracellular imaging

Author:
Lana ZouThese authors contributed equally to this work.; Qiong Wu; Yangjie Zhou; Xue Gong; Xiaoqing Liu; Fuan Wang
Source:
Chemical communications 2019 v.55 no.46 pp. 6519-6522
ISSN:
1364-548X
Subject:
biocatalysts; catalytic activity; chemical compounds; chemical reactions; image analysis; microRNA
Abstract:
... A facile cross-catalytic circuit is engineered for intracellular microRNA (miRNA) imaging by facilely integrating a catalytic hairpin assembly (CHA) amplifier and DNAzyme biocatalyst through an ingenious feedback loop. These two indispensable catalytic reactions play vital roles in executing high-performance signal amplification, as demonstrated experimentally and theoretically. ...
DOI:
10.1039/c9cc02637a

3. A Review on Microbial Alkaline Protease: An Essential Tool for Various Industrial Approaches

Author:
Mayuri Sharma; Yogesh Gat; Shalini Arya; Vikas Kumar; Anil Panghal; Ashwani Kumar
Source:
Industrial biotechnology 2019 v.15 no.2 pp. 69-78
ISSN:
1931-8421
Subject:
amino acids; biocatalysts; biotechnology; cell growth; detergents; fungi; leather industry; pH; peptides; proteinases; proteins; temperature
Abstract:
... Proteolytic enzymes are present in all living organisms and help in cell growth and differentiation. Proteases are the hydrolytic enzymes that act as biocatalysts for the cleavage of proteins into smaller peptides and amino acids. Microorganisms have turned out to be a competent and inexpensive source of alkaline protease enzymes that can produce a continuous and consistent supply of desired produ ...
DOI:
10.1089/ind.2018.0032

4. A Review on Microbial Electrocatalysis Systems Coupled with Membrane Bioreactor to Improve Wastewater Treatment

Author:
Jicun Wang; Shuai Zhao; Apurva Kakade; Saurabh Kulshreshtha; Pu Liu; Xiangkai Li
Source:
Microorganisms 2019 v.7 no.10 pp. -
ISSN:
2076-2607
Subject:
biocatalysts; catalytic activity; electric power; electrochemistry; energy; fouling; membrane bioreactors; microbial fuel cells; microorganisms; wastewater treatment
Abstract:
... Microbial electrocatalysis is an electro reaction that uses microorganisms as a biocatalyst, mainly including microbial electrolytic cells (MEC) and microbial fuel cells (MFC), which has been used for wastewater treatment. However, the low processing efficiency is the main drawback for its practical application and the additional energy input of MEC system results in high costs. Recently, MFC/MEC ...
DOI:
10.3390/microorganisms7100372
PubMed:
31547014
PubMed Central:
PMC6843282

5. A biocatalyst for sustainable wax ester production: re-wiring lipid accumulation in Rhodococcus to yield high-value oleochemicals

Author:
James W. Round; Raphael Roccor; Lindsay D. Eltis
Source:
Green chemistry 2019 v.21 no.23 pp. 6468-6482
ISSN:
1463-9270
Subject:
Marinobacter hydrocarbonoclasticus; Rhodococcus jostii; acyl coenzyme A; bacteria; batch fermentation; biocatalysts; carbon; catalytic activity; enzymes; genetic vectors; glucose; green chemistry; lipids; promoter regions; specific growth rate; wax esters
Abstract:
... Oleaginous microorganisms, such as those found in the Rhodococcus genus, have considerable potential for the sustainable production of lipid-based chemicals. Herein, we rewired lipid accumulation in Rhodococcus jostii RHA1 to create an industrially viable biocatalyst for the production of high-value wax esters (WEs). To efficiently manipulate these non-model bacteria, we first expanded the genetic ...
DOI:
10.1039/c9gc03228b

6. A biocatalytic redox cascade approach for one-pot deracemization of carboxyl-substituted tetrahydroisoquinolines by stereoinversion

Author:
Shuyun Ju; Mingxin Qian; Jing Li; Gang Xu; Lirong Yang; Jianping Wu
Source:
Green chemistry 2019 v.21 no.20 pp. 5579-5585
ISSN:
1463-9270
Subject:
D-amino-acid oxidase; Fusarium solani; Pseudomonas putida; amino acids; biocatalysis; biocatalysts; biotransformation; carboxylic acids; chemical industry; enantiomers; enantioselectivity; green chemistry; oxidation; reducing agents; synthesis; tetrahydroisoquinolines
Abstract:
... Optically pure 1,2,3,4-tetrahydroisoquinoline carboxylic acids are important chiral building blocks in the pharmaceutical and fine chemical industries. However, the existing chemo-enzymatic deracemization method employing d-amino acid oxidase from Fusarium solani M-0718 (FsDAAO) suffers from the requirement for a large excess of a nonselective chemical reducing agent. To explore an alternative met ...
DOI:
10.1039/c9gc02795e

7. A co-immobilization of pectinase and cellulase onto magnetic nanoparticles for antioxidant extraction from waste fruit peels

Author:
Shamraja S. Nadar; Virendra K. Rathod
Source:
Biocatalysis and agricultural biotechnology 2019 v.17 pp. 470-479
ISSN:
1878-8181
Subject:
Fourier transform infrared spectroscopy; X-ray diffraction; antioxidant activity; bananas; bioactive compounds; biocatalysts; cell walls; crosslinking; endo-1,4-beta-glucanase; free radical scavengers; fruit peels; fruits; glutaraldehyde; half life; immobilized enzymes; magnetism; mangoes; nanoparticles; pectins; polygalacturonase; scanning electron microscopy; solvents; temperature; thermal stability; thermogravimetry
Abstract:
... The major obstruction in efficient extraction of intracellular bioactive components is highly complex cell wall structure which is made up of rigid polysaccharides. An enhancement in the release of bioactive compounds from plants and fruits can be facilitated by hydrolysing cellular cellulosic material along with pectin. In this context, co-immobilized magnetic nanobiocatalyst was prepared by simu ...
DOI:
10.1016/j.bcab.2018.12.015

8. A comprehensive review of biodiesel production methods from various feedstocks

Author:
R. Selvaraj; R. Praveenkumar; I. Ganesh Moorthy
Source:
Biofuels 2019 v.10 no.3 pp. 325-333
ISSN:
1759-7277
Subject:
Fourier transform infrared spectroscopy; algal oils; algorithms; animal fats and oils; biocatalysts; biodiesel; carboxylic ester hydrolases; catalytic activity; cooking fats and oils; energy; fatty acid methyl esters; feedstocks; free fatty acids; fuel production; gas chromatography-mass spectrometry; magnetism; methanol; mixing; nanocatalysts; nanoparticles; neural networks; pH; response surface methodology; surveys; temperature; transesterification; transmission electron microscopy; ultrasonics; viscosity
Abstract:
... This article reviews the performance of biodiesel production from various feedstocks and analyzes the associated challenges. The existing literature survey dealt with the potential and important feedstocks like edible oil, non-edible oil, animal fat and algae oil for the biodiesel production. The result shows that the various sources have different yield due to processes variables. The yield of bi ...
DOI:
10.1080/17597269.2016.1204584

9. A facile route to preparation of immobilized cellulase on polyurea microspheres for improving catalytic activity and stability

Author:
Ying Sui; Yu Cui; Guangming Xia; Xiujing Peng; Guangzheng Yuan; Guoxin Sun
Source:
Process biochemistry 2019 v.87 pp. 73-82
ISSN:
1359-5113
Subject:
Fourier transform infrared spectroscopy; ambient temperature; atomic force microscopy; biocatalysts; catalytic activity; cellulose; chemical industry; dimethyl sulfoxide; endo-1,4-beta-glucanase; enzyme activity; hydrolysis; mass transfer; microparticles; mixing; pH; porous media; scanning electron microscopy; sustainable technology; thermal stability; thermogravimetry
Abstract:
... The input-output ratio of the immobilization process is crucial to determining whether the immobilized cellulase can truly participate in industrial production. This study reports a simple, efficient and environmentally friendly method for covalent immobilization of cellulase on uniform, monodisperse polyurea microspheres. The reported method offers several benefits including (i) polyurea microsph ...
DOI:
10.1016/j.procbio.2019.09.002

10. A far-red fluorescent probe for sensing laccase in fungi and its application in developing an effective biocatalyst for the biosynthesis of antituberculous dicoumarin

Author:
Zhenhao TianThese authors contributed equally to this work.; Qingsong Yan; Lei Feng; Sa Deng; Chao Wang; Jingnan Cui; Chunjie Wang; Zhanjun Zhang; Tony D. James; Xiaochi Ma
Source:
Chemical communications 2019 v.55 no.27 pp. 3951-3954
ISSN:
1364-548X
Subject:
Mycobacterium tuberculosis; Rhizopus oryzae; biocatalysts; biosynthesis; chemical reactions; fluorescent dyes; fungi; laccase
Abstract:
... A far-red fluorescent probe has been developed for sensing fungal laccase. The probe was used to determine that Rhizopus oryzae had a high level endogenous laccase amongst 24 fungal strains. The Rhizopus oryzae was then used as a biocatalyst for the preparation of dicoumarin resulting in significant inhibition of Mycobacterium tuberculosis H37Ra. ...
DOI:
10.1039/c9cc01579e

11. A fluorescence-based activity assay for immobilized lipases in non-native media

Author:
Kim N. Ingenbosch; Anna Rousek; Dennis S. Wunschik; Kerstin Hoffmann-Jacobsen
Source:
Analytical biochemistry 2019 v.569 pp. 22-27
ISSN:
0003-2697
Subject:
Bacillus subtilis; Pseudozyma antarctica; acetonitrile; biocatalysts; carboxylic ester hydrolases; enzyme activity; ethanol; fluorescence; hydrolysis; prediction; solvents
Abstract:
... A new method for the analysis of lipase activity in the immobilized state is developed. The fluorescence assay aims to quantify the potential of lipases for the application in organic solvents. As lipases are universally immobilized on polymeric carriers for the use in bioorganic synthesis, the assay includes an immobilization step on the walls of polymeric cuvettes. The activity of the immobilize ...
DOI:
10.1016/j.ab.2019.01.005

12. A generic platform for the immobilisation of engineered biocatalysts

Author:
Matthew P. Thompson; Sasha R. Derrington; Rachel S. Heath; Joanne L. Porter; Juan Mangas-Sanchez; Paul N. Devine; Matthew D. Truppo; Nicholas J. Turner
Source:
Tetrahedron 2019 v.75 no.3 pp. 327-334
ISSN:
0040-4020
Subject:
biocatalysis; biocatalysts; case studies; chemical reactions; chemical structure; enzyme stability; glass; immobilized enzymes; industrial applications; organic compounds; pharmaceutical industry; porosity
Abstract:
... The application of biocatalysis in the pharmaceutical industry is rapidly growing as a result of increased access to enzymes that meet the demands of industrial processes. This expansion of activity has led to a corresponding increase in the demand for immobilised enzymes. EziG™ (EnginZyme AB, Sweden) is marketed as a general matrix for enzyme immobilisation on controlled porosity glass. In this w ...
DOI:
10.1016/j.tet.2018.12.004

13. A new cold-active and alkaline pectate lyase from Antarctic bacterium with high catalytic efficiency

Author:
Yumeng Tang; Pan Wu; Sijing Jiang; Jonathan Nimal Selvaraj; Shihui Yang; Guimin Zhang
Source:
Applied microbiology and biotechnology 2019 v.103 no.13 pp. 5231-5241
ISSN:
0175-7598
Subject:
Escherichia coli; Massilia; bacteria; biocatalysts; catalytic activity; cold; cost effectiveness; energy; genes; industry; mutational analysis; pH; pectate lyase; polygalacturonase; polygalacturonic acid; temperature; Antarctic region
Abstract:
... Cold-active enzymes have become attractive biocatalysts in biotechnological applications for their ability to retain high catalytic activity below 30 °C, which allows energy reduction and cost saving. Here, a 1041 bp gene pel1 encoding a 34.7 KDa pectate lyase was cloned from a facultatively psychrophilic Antarctic bacterium Massilia eurypsychrophila and heterologously expressed in Escherichia col ...
DOI:
10.1007/s00253-019-09803-1

14. A new laccase-mediator system facing the biodegradation challenge: Insight into the NSAIDs removal

Author:
Azzurra Apriceno; Maria Luisa Astolfi; Anna Maria Girelli; Francesca Romana Scuto
Source:
Chemosphere 2019 v.215 pp. 535-542
ISSN:
0045-6535
Subject:
Coriolus versicolor; aquatic environment; biocatalysts; chitosan; diclofenac; enzyme activity; hydroxylation; ketoprofen; laccase; pH; pollutants
Abstract:
... Non-steroidal anti-inflammatory drugs (NSAIDs) are widely found pollutants in the aquatic environment and the currently available treatments for their removal are usually associated with some drawbacks. The aim of this research was to apply a laccase-mediator system for the degradation of some commonly used NSAIDs, namely diclofenac (DCF), naproxen (NAP) and ketoprofen (KP). The biocatalyst was ob ...
DOI:
10.1016/j.chemosphere.2018.10.086

15. A novel Bacillus sp. isolated from rotten seaweed: Identification and characterization alginate lyase its produced

Author:
DEWI SESWITA ZILDA; YULIYANTI YULIANTI; RIZKY FAUZIYAH SHOLIHAH; SUBARYONO SUBARYONO; YUSRO NURI FAWZYA; HARI EKO IRIANTO
Source:
Biodiversitas 2019 v.20 no.4 pp. 1166-1172
ISSN:
2085-4722
Subject:
Bacillus (bacteria); EDTA (chelating agent); alginate lyase; bacteria; biocatalysts; calcium; enzyme activity; fluorides; macroalgae; medicinal properties; nucleotide sequences; oligosaccharides; pH; serine; sodium alginate; temperature
Abstract:
... Zilda DS, Yulianti Y, Sholihah RF, Subaryono S, Fawzya YN, Irianto HE.2019. A novel Bacillus sp. isolated from rotten seaweed: identification and characterization alginate lyase its produced. Biodiversitas 20: 1166-1172. Alginate lyase has been known as potential biocatalyst not only for industrial but also medicinal application especially for the production of oligosaccharides which have distinct ...
DOI:
10.13057/biodiv/d200432

16. A novel process for biodiesel production from sludge palm oil

Author:
Papasanee Muanruksa; James Winterburn; Pakawadee Kaewkannetra
Source:
MethodsX 2019 v.6 pp. 2838-2844
ISSN:
2215-0161
Subject:
biocatalysts; biodiesel; carboxylic ester hydrolases; catalytic activity; esterification; fatty acid composition; fatty acids; free fatty acids; fuel production; glycerol; oil refining; palm oils; sludge; soaps
Abstract:
... Typically, sludge palm oil (SPO) which discharged from the palm oil refining process, is a low cost material of potential value, due to its high free fatty acid content. Accordingly, there is potential for upgrading low grade oil into valuable biofuel. In this work, we present a novel method for biodiesel production from SPO. The process consists of two steps (i) free fatty acid (FFA) extraction ( ...
DOI:
10.1016/j.mex.2019.09.039
PubMed:
31871917
PubMed Central:
PMC6911963

17. A novel process for phosphatidylserine production using a Pichia pastoris whole-cell biocatalyst with overexpression of phospholipase D from Streptomyces halstedii in a purely aqueous system

Author:
Yihan Liu; Lin Huang; Yu Fu; Dong Zheng; Jieying Ma; Yanzhen Li; Zehua Xu; Fuping Lu
Source:
Food chemistry 2019 v.274 pp. 535-542
ISSN:
0308-8146
Subject:
biocatalysts; phosphatidylserines; serine; Komagataella pastoris; gene overexpression; phosphatidylcholines; Streptomyces halstedii; food industry; genes; pH; temperature; catalytic activity; phospholipase D
Abstract:
... This work was aimed to develop a novel phosphatidylserine (PS) production process for the food industry. The pldsh gene, encoding phospholipase D from Streptomyces halstedii (PLDsh) was cloned, and the codon optimized pldmsh gene was freely expressed by Pichia pastoris GS115 and successfully overexpressed on the cell surface of P. pastoris GS115 as displayed PLDMsh (dPLDMsh) – a whole-cell biocata ...
DOI:
10.1016/j.foodchem.2018.08.105

18. A novel self-sufficient biocatalyst based on transaminase and pyridoxal 5′-phosphate covalent co-immobilization and its application in continuous biosynthesis of sitagliptin

Author:
Xiao-Jian Zhang; Hao-Hao Fan; Nan Liu; Xin-Xin Wang; Feng Cheng; Zhi-Qiang Liu; Yu-Guo Zheng
Source:
Enzyme and microbial technology 2019 v.130 pp. 109362
ISSN:
0141-0229
Subject:
amines; biocatalysts; biosynthesis; pyridoxal; pyridoxal phosphate; space and time; transaminases
Abstract:
... Transaminases (TAs, EC 2.6.1.X) are a class of promising catalysts for the synthesis of chiral amines. The addition of exogenous expensive cofactor, pyridoxal 5′-phosphate (PLP) leads to the high cost and complicated reaction composition and thus limits the industrial implementation of TAs. In this study, a novel self-sufficient biocatalyst was developed based on transaminase and PLP covalent co-i ...
DOI:
10.1016/j.enzmictec.2019.109362

19. A novel short chain dehydrogenase from Bacillus megaterium for the conversion of the sesquiterpene nootkatol to (+)-nootkatone

Author:
Mohammed Milhim; Philip Hartz; Adrian Gerber; Rita Bernhardt
Source:
Journal of biotechnology 2019 v.301 pp. 52-55
ISSN:
0168-1656
Subject:
Bacillus megaterium; biocatalysts; biotechnology; flavor; grapefruits; industry; ingredients; odors; oxidoreductases; sesquiterpenoids; smell
Abstract:
... (+)-Nootkatone is a natural ingredient that occurs in grapefruit and certain other plants and is responsible for the characteristic smell of grapefruit. Due to its versatile applications in the flavor and fragrance industry as well as its application in some medical uses it recruits the interests of academic research along with industrial biotechnology. In the current work we present the applicati ...
DOI:
10.1016/j.jbiotec.2019.05.017

20. A novel starch-binding laccase from the wheat pathogen Zymoseptoria tritici highlights the functional diversity of ascomycete laccases

Author:
Majid Haddad Momeni; Paolo Bollella; Roberto Ortiz; Esben Thormann; Lo Gorton; Maher Abou Hachem
Source:
BMC biotechnology 2019 v.19 no.1 pp. 61
ISSN:
1472-6750
Subject:
Basidiomycota; Mycosphaerella graminicola; active sites; biocatalysts; carbohydrate binding; databases; functional diversity; laccase; lignocellulose; oxidation; oxygen; phylogeny; plant pathogenic fungi; redox potential; saprophytes; wheat
Abstract:
... BACKGROUND: Laccases are multicopper oxidases, which are assigned into auxiliary activity family 1 (AA1) in the CAZy database. These enzymes, catalyzing the oxidation of phenolic and nonphenolic substrates coupled to reduction of O₂ to H₂O, are increasingly attractive as eco-friendly oxidation biocatalysts. Basidiomycota laccases are well characterized due to their potential in de-lignification of ...
DOI:
10.1186/s12896-019-0552-4
PubMed:
31426777
PubMed Central:
PMC6700816

21. A novel β-xylosidase from Anoxybacillus sp. 3M towards an improved agro-industrial residues saccharification

Author:
Loredana Marcolongo; Francesco La Cara; Giovanni del Monaco; Susana M. Paixão; Luís Alves; Isabel Paula Marques; Elena Ionata
Source:
International journal of biological macromolecules 2019 v.122 pp. 1224-1234
ISSN:
0141-8130
Subject:
Anoxybacillus; aluminum; beta-mercaptoethanol; biocatalysts; biotransformation; catalytic activity; copper; half life; iron; mercury; pH; saccharification; silver; spent grains; xylan 1,4-beta-xylosidase; xylooligosaccharides; xylose
Abstract:
... An intracellular β-xylosidase (AbXyl), from the thermoalkaline Anoxybacillus sp. 3M, was purified and characterized. The homodimeric enzyme (140 kDa) was optimally active at 65 °C and pH 5.5, exhibited half life of 10 h at 60 °C, 78 and 88% residual activity after 24 h, at pH 4.5 and 8.0, respectively. Fe2+, Cu2+, Al3+, Ag+ and Hg2+ inhibited the enzyme; the activity was moderately stimulated by S ...
DOI:
10.1016/j.ijbiomac.2018.09.075

22. A perspective on the biotechnological applications of the versatile tyrosinase

Author:
Kyoungseon Min; Gwon Woo Park; Young Je Yoo; Jin-Suk Lee
Source:
Bioresource technology 2019 v.289 pp. 121730
ISSN:
0960-8524
Subject:
L-dopa; biocatalysts; biorefining; bioremediation; biosensors; catechol oxidase; delignification; enzyme activity; industrial applications; oxidation; pollutants; polyphenols; quinones
Abstract:
... Tyrosinase (E.C. 1.14.18. 1) is a type of Cu-containing oxidoreductase which has bifunctional activity for various phenolic substrates: ortho-hydroxylation of monophenols to diphenols (a cresolase activity) and oxidation of diphenols to quinones (a catecholase activity). Based on the broad substrate spectrum, tyrosinase has been used in bioremediation of phenolic pollutants, constructing biosensor ...
DOI:
10.1016/j.biortech.2019.121730

23. A promising laccase immobilization approach for Bisphenol A removal from aqueous solutions

Author:
Fatiha Lassouane; Hamid Aït-Amar; Saïd Amrani; Susana Rodriguez-Couto
Source:
Bioresource technology 2019 v.271 pp. 360-367
ISSN:
0960-8524
Subject:
Fourier transform infrared spectroscopy; Trametes pubescens; adsorption; aqueous solutions; biocatalysts; bisphenol A; calcium alginate; crosslinking; glutaraldehyde; laccase; pH
Abstract:
... The immobilization of crude laccase from Trametes pubescens by glutaraldehyde crosslinking prior to entrapment into Ca-alginate beads increased the immobilization yield by 30% and reduced the leaking by 7-fold compared to the immobilization with no crosslinking. The performance of the newly developed biocatalyst to degrade Bisphenol A (BPA) from aqueous solutions was tested. Thus, operating at opt ...
DOI:
10.1016/j.biortech.2018.09.129

24. Acetylacetone extends the working life of laccase in enzymatic transformation of malachite green by interfering with a key intermediate

Author:
Wenguang Huang; Hua Yang; Shujuan Zhang
Source:
Journal of hazardous materials 2019 v.366 pp. 520-528
ISSN:
0304-3894
Subject:
biocatalysts; laccase; malachite green; statistical models
Abstract:
... The potential of acetylacetone (AA) as a mediator of laccase has been tested in the enzymatic transformation of malachite green (MG). AA inhibited the laccase-induced transformation of MG at the beginning of incubation but extended the working life of laccase in long runs. To elucidate the underlying mechanisms, the transformation of MG in the laccase-AA system was systematically investigated. The ...
DOI:
10.1016/j.jhazmat.2018.12.028

25. Acoustic cavitation assisted lipase B catalysed synthesis of polyethylene glycol stearate in a solvent free system via esterification: synthesis and optimization

Author:
Girish S Nhivekar; Virendra K Rathod
Source:
Journal of chemical technology and biotechnology 2019 v.94 no.8 pp. 2465-2474
ISSN:
0268-2575
Subject:
Pseudozyma antarctica; acoustics; agitation; biocatalysts; carboxylic ester hydrolases; catalytic activity; esterification; immobilized enzymes; mixing; polyethylene glycol; sieves; solvents; stearic acid; temperature; ultrasonics
Abstract:
... BACKGROUND: The current work describes the application of ultrasound and its improving effect on the synthesis of polyethylene glycol (PEG) stearate using Fermase (Fermase CALB™10,000 Candida antarctica Lipase B) as a biocatalyst via esterification of PEG600 and stearic acid. The influence of various parameters like molar ratio, temperature, speed of agitation, enzyme loading, effect of addition o ...
DOI:
10.1002/jctb.6030

26. Advances in cloning, structural and bioremediation aspects of nitrile hydratases

Author:
K. Supreetha; Saroja Narsing Rao; D. Srividya; H. S. Anil; S. Kiran
Source:
Molecular biology reports 2019 v.46 no.4 pp. 4661-4673
ISSN:
0301-4851
Subject:
acrylamides; biocatalysts; bioinformatics; bioremediation; biotransformation; catalytic activity; crystal structure; enantioselectivity; gene expression; genes; industry; metabolism; metagenomics; microorganisms; nicotinamide; nitrile hydratase; nitriles; thermal stability; toxicity
Abstract:
... Nitrile hydratase (NHase) is a prominent enzyme in many microorganisms for its nitrile metabolism. The potentiality in the bioconversion of nitriles to its high-value amides has been extensively used in industries for the production of acrylamide and nicotinamide which are essential chemicals. Enzymologists are still considering NHases for its potential biotechnological applications including biot ...
DOI:
10.1007/s11033-019-04811-w

27. Agarose-based microwell array chip for high-throughput screening of functional microorganisms

Author:
Leicheng Zhang; Peng Chen; Zhuoyue Zhou; Yawei Hu; Qiuyue Sha; Houjin Zhang; Xin Liu; Wei Du; Xiaojun Feng; Bi-Feng Liu
Source:
Talanta 2019 v.191 pp. 342-349
ISSN:
0039-9140
Subject:
agarose; bacteria; bacterial communities; biocatalysts; carboxylic ester hydrolases; enzymatic reactions; fluorescence; fluorescent dyes; hydrolysis; irradiation; new species; phenotype; rhodamines; ribosomal RNA; screening; sequence analysis; synthetic biology; ultraviolet radiation
Abstract:
... Natural bacterial communities provide a rich source of biocatalysts, however, high-throughput screening for the functional bacteria remains a major challenge. Here, we present an agarose-based microwells array chip for functionally screening and isolating novel microorganisms with merits of high-throughput, high specificity and sensitivity. In this approach, the bacterial cells were loaded with si ...
DOI:
10.1016/j.talanta.2018.08.090

28. Agarose-chitosan hydrogel-immobilized horseradish peroxidase with sustainable bio-catalytic and dye degradation properties

Author:
Muhammad Bilal; Tahir Rasheed; Yuping Zhao; Hafiz M.N. Iqbal
Source:
International journal of biological macromolecules 2019 v.124 pp. 742-749
ISSN:
0141-8130
Subject:
biocatalysts; catalytic activity; dyes; functional properties; high performance liquid chromatography; hydrogels; oxidation; pH; peroxidase; scanning electron microscopy; temperature
Abstract:
... Herein, we developed and characterized robust agarose–chitosan hydrogel using N‑hydroxysuccinimide (NHS) as a mild chemical cross-linker. The hydrogel offered a simple, effective and eco-friendlier support material with >90% of immobilization efficiency of horseradish peroxidase. The surface morphology and functional properties of the agarose–chitosan hydrogel with and without immobilized horserad ...
DOI:
10.1016/j.ijbiomac.2018.11.220

29. Amination of ficin extract to improve its immobilization on glyoxyl-agarose: Improved stability and activity versus casein

Author:
El-Hocine Siar; Roberto Morellon-Sterling; Mohammed Nasreddine Zidoune; Roberto Fernandez-Lafuente
Source:
International journal of biological macromolecules 2019 v.133 pp. 412-419
ISSN:
0141-8130
Subject:
agarose; amination; biocatalysts; casein; enzyme activity; ethylenediamines; ficain; hydrolysis; pH; urea
Abstract:
... Ficin extract has been aminated using ethylenediamine and carbodiimide to transform all exposed carboxylic groups into amino groups, retaining around 80% of activity versus benzoyl-d,l-arginine p-nitroanilide hydrochloride (BANA) and 90% versus casein. This aminated enzyme was then immobilized on glyoxyl agarose beads. After optimization of the immobilization protocol (immobilization at pH 10 for ...
DOI:
10.1016/j.ijbiomac.2019.04.123

30. Aminobacter sp. MSH1 Mineralizes the Groundwater Micropollutant 2,6-Dichlorobenzamide through a Unique Chlorobenzoate Catabolic Pathway

Author:
Bart Raes; Benjamin Horemans; Daniel Rentsch; Jeroen T’Syen; Maarten G. K. Ghequire; René De Mot; Ruddy Wattiez; Hans-Peter E. Kohler; Dirk Springael
Source:
Environmental science & technology 2019 v.53 no.17 pp. 10146-10156
ISSN:
1520-5851
Subject:
Aminobacter; amidase; biocatalysts; bioremediation; carbon; catabolism; chlorine; dehalogenation; energy; groundwater; hydroxylation; nitrogen; pollutants; tricarboxylic acid cycle
Abstract:
... 2,6-Dichlorobenzamide (BAM) is a major groundwater micropollutant posing problems for drinking water treatment plants (DWTPs) that depend on groundwater intake. Aminobacter sp. MSH1 uses BAM as the sole source of carbon, nitrogen, and energy and is considered a prime biocatalyst for groundwater bioremediation in DWTPs. Its use in bioremediation requires knowledge of its BAM-catabolic pathway, whic ...
DOI:
10.1021/acs.est.9b02021

31. An antimonene/Cp*Rh(phen)Cl/black phosphorus hybrid nanosheet-based Z-scheme artificial photosynthesis for enhanced photo/bio-catalytic CO₂ reduction

Author:
Xiaoyuan Ji; Yong Kang; Taojian Fan; Qingqing Xiong; Songping Zhang; Wei Tao; Han Zhang
Source:
Journal of materials chemistry A 2019 v.8 no.1 pp. 323-333
ISSN:
2050-7496
Subject:
NAD (coenzyme); biocatalysis; biocatalysts; carbon dioxide; electrons; electrostatic interactions; enzymes; formic acid; hydrophobic bonding; hydrophobicity; light; nanosheets; oxidation; phosphorus; photocatalysis; photosynthesis; polymers; value-added products
Abstract:
... Developing a biocatalyzed artificial photosynthesis system (APS) with a Z-scheme for CO₂ conversion has a promising outlook. However, it remains unexplored and challenging. Herein, we present an antimonene (AM) and black phosphorus (BP) hybrid nanosheet (HNSs) based Z-scheme APS for enhanced photo/bio-catalytic CO₂ reduction. An “amphipathic” polymer PEI-PEG-C18 with a positively charged PEI-head ...
DOI:
10.1039/c9ta11167k

32. An effective hybrid strategy for converting rice straw to furoic acid by tandem catalysis via Sn-sepiolite combined with recombinant E. coli whole cells harboring horse liver alcohol dehydrogenase

Author:
Bo Peng; Cui-Luan Ma; Peng-Qi Zhang; Chang-Qing Wu; Zi-Wei Wang; Ai-Tao Li; Yu-Cai He; Bin Yang
Source:
Green chemistry 2019 v.21 no.21 pp. 5914-5923
ISSN:
1463-9270
Subject:
Escherichia coli; alcohol dehydrogenase; alkali treatment; biocatalysts; biomass; catalytic activity; furfural; green chemistry; horses; liver; pH; rice straw; sepiolite
Abstract:
... The upgrading of biomass-derived furfural into high-value bio-based chemicals has attracted interest. In this case, the conversion of rice straw into furfural was firstly performed using tin-loaded sepiolite (Sn-sepiolite) as a catalyst. Acidified solid acid Sn-sepiolite (3.0 wt%) converted alkali-pretreated dewaxed rice straw into furfural at 42.2% yield at 170 °C for 20 min. Moreover, biomass-de ...
DOI:
10.1039/c9gc02499a

33. Anaerobic Conversion of Lignocellulose to Materials for Biofuel Production: Volatile Fatty Acids and Ethanol

Author:
M. A. Gladchenko; S. N. Gaydamaka; V. P. Murygina; S. D. Varfolomeev
Source:
Applied biochemistry and microbiology 2019 v.55 no.7 pp. 756-764
ISSN:
0003-6838
Subject:
Clostridium acetobutylicum; acetic acids; acid hydrolysis; bacteria; biocatalysts; biomass; butyric acid; chemical oxygen demand; depolymerization; ethanol; fuel production; glycerol; hydrolysates; lignin; lignocellulose; reducing sugars; sawdust; volatile fatty acids; wheat straw
Abstract:
... The processes of oxidative depolymerization and acidic hydrolysis have consistently been used for the pretreatment of lignocellulosic biomass (wheat straw, sawdust, and lignin), which makes it possible to obtain a high content of soluble organic compounds in the hydrolysate (44–94 g COD/L) and to enhance the concentration of reducing sugars from 1 to 36%. The introduction of Clostridium acetobutyl ...
DOI:
10.1134/S0003683819070020

34. Anti-selective direct asymmetric Mannich reaction catalyzed by protease

Author:
Yu-Jue Chen; Yang Xiang; Yan-Hong He; Zhi Guan
Source:
Tetrahedron letters 2019 v.60 no.15 pp. 1066-1071
ISSN:
0040-4039
Subject:
Streptomyces griseus; aldehydes; biocatalysts; chemical reactions; chemical structure; diastereoselectivity; enantioselectivity; proteinases
Abstract:
... The anti-selective direct asymmetric Mannich reaction of (hetero) aromatic aldehydes, 4-anisidine and O-protected hydroxyacetones for the synthesis of stereodefined anti-β-amino-α-hydroxycarbonyl compounds was developed. Protease type XIV from Streptomyces griseus (SGP) was used as a biocatalyst in 1,4-dioxane/phosphate buffer under mild reaction conditions. The excellent diastereoselectivities of ...
DOI:
10.1016/j.tetlet.2019.03.031

35. Application of Immobilized Enzymes in Food Industry

Author:
Ekaterina D. Yushkova; Elena A. Nazarova; Anna V. Matyuhina; Alina O. Noskova; Darya O. Shavronskaya; Vladimir V. Vinogradov; Natalia N. Skvortsova; Elena F. Krivoshapkina
Source:
Journal of agricultural and food chemistry 2019 v.67 no.42 pp. 11553-11567
ISSN:
1520-5118
Subject:
biocatalysts; biocompatibility; cost effectiveness; food industry; immobilized enzymes; thermal stability
Abstract:
... Enzymes are macromolecular biocatalysts, widely used in food industry. In applications, enzymes are often immobilized on inert and insoluble carriers, which increase their efficiency due to multiple reusability. The properties of immobilized enzymes depend on the immobilization method and the carrier type. The choice of the carrier usually concerns the biocompatibility, chemical and thermal stabil ...
DOI:
10.1021/acs.jafc.9b04385

36. Application of a Multienzymatic System from Natural Latex in Key Reactions for oil-Based Biorefineries

Author:
Daniel Alberto Sánchez; Susana Raquel Morcelle; María Elisa Fait; Gabriela Marta Tonetto; María Luján Ferreira
Source:
Fermentation 2019 v.5 no.1 pp. -
ISSN:
2311-5637
Subject:
Araujia; biocatalysts; biorefining; esterification; fruits; hydrolysis; latex; oleic acid; soybean oil; triacylglycerols
Abstract:
... Oil-based biorefineries play a crucial role in the production of key platform chemicals that can be generated via biotechnological processes instead of a petrochemical route. This work focuses on the latex of the fruit of Araujia sericifera, which can be considered a multienzymatic system with applications in key reactions in oil-based biorefineries. The latex of Araujia sericifera (ASL) was used ...
DOI:
10.3390/fermentation5010018

37. Assessment of Ready-to-Use Freeze-dried Immobilized Biocatalysts as Innovative Starter Cultures in Sourdough Bread Making

Author:
Ioanna Mantzourani; Antonia Terpou; Athanasios Alexopoulos; Eugenia Bezirtzoglou; Stavros Plessas
Source:
Foods 2019 v.8 no.1 pp. -
ISSN:
2304-8158
Subject:
Lactobacillus delbrueckii subsp. bulgaricus; Lactobacillus paracasei; acetic acid; acidity; biocatalysts; breadmaking; cheese whey; cryoprotectants; freeze drying; gas chromatography-mass spectrometry; lactic acid; microorganisms; sodium hydroxide; sourdough bread; spoilage; starter cultures; volatile compounds; wheat bran
Abstract:
... In the present study the effect of innovative biocatalysts as starter cultures in sourdough bread making was explored. The biocatalysts consisted of Lactobacillus paracasei K5 and Lactobacillus bulgaricus ATCC 11842 (in single and mixed form), immobilized on delignified wheat bran (DWB), and freeze dried without cryoprotectants. The parameters monitored were physicochemical characteristics, mold a ...
DOI:
10.3390/foods8010040
PubMed:
30669666
PubMed Central:
PMC6352066

38. Atypical organic-solvent tolerant bacterial hormone sensitive lipase-like homologue EstAG1 from Staphylococcus saprophyticus AG1: Synthesis and characterization

Author:
Alisa Gricajeva; Ingrida Bikutė; Lilija Kalėdienė
Source:
International journal of biological macromolecules 2019 v.130 pp. 253-265
ISSN:
0141-8130
Subject:
Escherichia coli; Staphylococcus saprophyticus; bacteria; biocatalysts; biodiesel; biotechnology; carboxylic ester hydrolases; dimethyl sulfoxide; dioxane; esters; ethanol; flavor; leadership; markets; models; pH; polluted soils; sequence homology; Lithuania
Abstract:
... Biocatalysts exerting activity against ester bonds have a broad range of applications in modern biotechnology. Some of the most industrially relevant enzymes of this type are lipolytic and their market is predicted to uphold leadership up till 2024. In this study, a novel bacterial hormone-sensitive lipase-like (bHSL) family homologue, designated EstAG1, was discovered by mining gDNA of bacteria i ...
DOI:
10.1016/j.ijbiomac.2019.02.110

39. Awakening the Sleeping Carboxylase Function of Enzymes: Engineering the Natural CO₂-Binding Potential of Reductases

Author:
Iria Bernhardsgrütter; Kristina Schell; Dominik M. Peter; Farshad Borjian; David Adrian Saez; Esteban Vöhringer-Martinez; Tobias J. Erb
Source:
Journal of the American Chemical Society 2019 v.141 no.25 pp. 9778-9782
ISSN:
1520-5126
Subject:
Erythrobacter; active sites; biocatalysts; bioinformatics; biotechnology; carbon dioxide; carboxylation; enzymatic reactions; enzymes; proteins; synthetic biology
Abstract:
... Developing new carbon dioxide (CO₂) fixing enzymes is a prerequisite to create new biocatalysts for diverse applications in chemistry, biotechnology and synthetic biology. Here we used bioinformatics to identify a “sleeping carboxylase function” in the superfamily of medium-chain dehydrogenases/reductases (MDR), i.e. enzymes that possess a low carboxylation side activity next to their original enz ...
DOI:
10.1021/jacs.9b03431
PubMed:
31188584
PubMed Central:
PMC6650136

40. Bacillus subtilis immobilization in alginate microfluidic-based microparticles aiming to improve lipase productivity

Author:
Aline F. Oliveira; Reinaldo G. Bastos; Lucimara G. de la Torre
Source:
Biochemical engineering journal 2019 v.143 pp. 110-120
ISSN:
1369-703X
Subject:
Bacillus subtilis; alginates; bacteria; biocatalysts; bioprocessing; carboxylic ester hydrolases; immobilized cells; microfluidic technology; microparticles; nutrient transport
Abstract:
... Biocatalyst immobilization is an important strategy to improve bioprocess productivity. However, conventional immobilization techniques produce non-uniform particles of millimetric size that may affect the cellular response by limiting nutrient transport into the particles. Droplet-based microfluidics can be an alternative to generate homogeneous and micrometric particles resulting in a reduced nu ...
DOI:
10.1016/j.bej.2018.12.014

41. Bacterial Respiratory Chain Diversity Reveals a Cytochrome c Oxidase Reducing O₂ at Low Overpotentials

Author:
Xie Wang; Romain Clément; Magali Roger; Marielle Bauzan; Ievgen Mazurenko; Anne de Poulpiquet; Marianne Ilbert; Elisabeth Lojou
Source:
Journal of the American Chemical Society 2019 v.141 no.28 pp. 11093-11102
ISSN:
1520-5126
Subject:
Acidithiobacillus ferrooxidans; bacteria; biocatalysts; carbon; carbon nanofibers; cytochrome c; cytochrome-c oxidase; electrochemistry; electrodes; electron transfer; electron transport chain; hydrophobicity; oxygen; pH
Abstract:
... Cytochrome c oxidases (CcOs) are the terminal enzymes in energy-converting chains of microorganisms, where they reduce oxygen into water. Their affinity for O₂ makes them attractive biocatalysts for technological devices in which O₂ concentration is limited, but the high overpotentials they display on electrodes severely limit their applicative use. Here, the CcO of the acidophilic bacterium Acidi ...
DOI:
10.1021/jacs.9b03268

42. Bacterial nitrilases and their regulation

Author:
Varsha P. Chhiba-Govindjee; Chris W. van der Westhuyzen; Moira L. Bode; Dean Brady
Source:
Applied microbiology and biotechnology 2019 v.103 no.12 pp. 4679-4692
ISSN:
0175-7598
Subject:
Streptomyces; amides; biocatalysts; biochemical pathways; carboxylic acids; drugs; environmental factors; heterocyclic compounds; multigene family; nitrilase; nitriles; transcription factors
Abstract:
... Commercially, nitrilases are valuable biocatalysts capable of converting a diverse range of nitriles to carboxylic acids for the greener synthesis of chemicals and pharmaceuticals. Nitrilases are widespread in nature and are both important components of metabolic pathways and a response to environmental factors such as natural or manmade nitriles. Nitrilases are often grouped together on a genome ...
DOI:
10.1007/s00253-019-09776-1

43. Batch and continuous cultivation processes of Candida tropicalis TISTR 5306 for ethanol and pyruvate decarboxylase production in fresh longan juice with optimal carbon to nitrogen molar ratio

Author:
Rojarej Nunta; Charin Techapun; Kittisak Jantanasakulwong; Thanongsak Chaiyaso; Phisit Seesuriyachan; Julaluk Khemacheewakul; Chatchadaporn Mahakuntha; Kritsadaporn Porninta; Sumeth Sommanee; Ngoc T. Trinh; Noppol Leksawasdi
Source:
Journal of food process engineering 2019 v.42 no.6 pp. e13227
ISSN:
0145-8876
Subject:
Candida tropicalis; ammonium sulfate; batch systems; biocatalysts; biofuels; carbon; case studies; ethanol; fructose; fruit growing; fruit juices; glucose; longans; malt; marketing; nitrogen; nose; pulp; pyruvate decarboxylase; solvents; sucrose; yeasts; zero wastes; Thailand
Abstract:
... The carbon to nitrogen (C/N) molar ratio of 21.88 ± 0.20 in fresh juice from C‐grade longan (FLJ‐C) supplemented with ammonium sulfate (AMS) was optimal for cultivation of Candida tropicalis TISTR 5306 producing ethanol concentration of 24.0 ± 1.1 g/L and specific pyruvate decarboxylase (PDC) activity of 0.138 ± 0.001 U/mg protein. The highest ethanol concentration in a 10 L continuous process (34 ...
DOI:
10.1111/jfpe.13227

44. Benzyl butyrate esterification mediated by immobilized lipases: Evaluation of batch and fed-batch reactors to overcome lipase-acid deactivation

Author:
Alessandra Cristina de Meneses; Amanda Gomes Almeida Sá; Lindomar Alberto Lerin; Marcos Lúcio Corazza; Pedro Henrique Hermes de Araújo; Claudia Sayer; Débora de Oliveira
Source:
Process biochemistry 2019 v.78 pp. 50-57
ISSN:
1359-5113
Subject:
Pseudozyma antarctica; benzyl alcohol; biocatalysts; butyric acid; carboxylic ester hydrolases; esterification; immobilized enzymes
Abstract:
... In this work, benzyl butyrate was synthesized for the first time via biotechnological esterification of benzyl alcohol and butyric acid in a solvent-free system (SFS). The synthesis was maximized in batch reactors, considering a low substrates molar ratio of 1:1. Novozym 435, a well-known commercial immobilized lipase (Candida antarctica fraction B - Cal B), was the best biocatalyst in the benzyl ...
DOI:
10.1016/j.procbio.2018.12.029

45. Benzyl propionate synthesis by fed-batch esterification using commercial immobilized and lyophilized Cal B lipase

Author:
Alessandra Cristina de Meneses; Lindomar Alberto Lerin; Pedro Henrique Hermes Araújo; Cláudia Sayer; Débora de Oliveira
Source:
Bioprocess and biosystems engineering 2019 v.42 no.10 pp. 1625-1634
ISSN:
1615-7591
Subject:
Pseudozyma antarctica; benzyl alcohol; biocatalysts; carboxylic ester hydrolases; esterification; freeze drying; light microscopy; propionic acid; protein content
Abstract:
... In this work, a fed-batch approach was adopted to overcome propionic acid lipase inactivation effects in the benzyl propionate direct esterification mediated by lipases. The ester synthesis was performed using commercial immobilized (Novozym 435) and lyophilized form Candida antarctica fraction B lipase (Cal B) as biocatalysts of the esterification between benzyl alcohol and propionic acid in a so ...
DOI:
10.1007/s00449-019-02159-w

46. Bilirubin oxidase-based silica macrocellular robust catalyst for on line dyes degradation

Author:
Armand Roucher; Elodie Roussarie; Régis M. Gauvin; Jad Rouhana; Sébastien Gounel; Claire Stines-Chaumeil; Nicolas Mano; Rénal Backov
Source:
Enzyme and microbial technology 2019 v.120 pp. 77-83
ISSN:
0141-0229
Subject:
Bacillus pumilus; ambient temperature; bilirubin; bilirubin oxidase; biocatalysts; catalytic activity; decolorization; dyes; laccase; pH; porosity; silica; silicon; storage temperature; wastewater treatment
Abstract:
... We present a new heterogeneous biocatalyst based on the grafting of Bilirubin Oxidase from Bacillus pumilus into macrocellular Si(HIPE) materials dedicated to water treatment. Due to the host intrinsic high porosity and monolithic character, on-line catalytic process is reached. We thus used this biocatalyst toward uni-axial flux decolorizations of Congo Red and Remazol Brilliant Blue (RBBR) at tw ...
DOI:
10.1016/j.enzmictec.2018.10.004

47. Bio-electrochemical power generation in petrochemical wastewater fed microbial fuel cell

Author:
Sumaya Sarmin; Baranitharan Ethiraj; M. Amirul Islam; Asmida Ideris; Chin Sim Yee; Md. Maksudur Rahman Khan
Source:
Science of the total environment 2019 v.695 pp. 133820
ISSN:
0048-9697
Subject:
Methanobacterium; Pseudomonas; acclimation; acrylic acid; biocatalysts; biofilm; chemical oxygen demand; dielectric spectroscopy; electrodes; electron transfer; energy; metabolites; methanogens; microbial fuel cells; microbial growth; power generation; sludge; voltammetry; wastewater
Abstract:
... The petrochemical wastewater (PCW) from acrylic acid plants possesses a very high chemical oxygen demand (COD) due to the presence of acrylic acid along with other organic acids. The treatment of PCW by conventional aerobic and anaerobic methods is energy intensive. Therefore, the treatment of PCW with concurrent power generation by employing microbial fuel cell (MFC) could be a potential alternat ...
DOI:
10.1016/j.scitotenv.2019.133820

48. Biocatalysis in ionic liquids for lignin valorization: Opportunities and recent developments

Author:
Joseph C. Stevens; Jian Shi
Source:
Biotechnology advances 2019 v.37 no.8 pp. 107418
ISSN:
0734-9750
Subject:
biocatalysis; biocatalysts; biomass; biorefining; cellulose; depolymerization; directed evolution; economic sustainability; engineering; enzyme activity; feedstocks; hemicellulose; immobilized enzymes; industry; ionic liquids; lignin; lignocellulose; molecular weight; petroleum; protein engineering; sugars; temperature; thermal stability
Abstract:
... Lignin holds tremendous potential as a renewable feedstock for upgrading to a number of high-value chemicals and products that are derived from the petroleum industry at present. Since lignin makes up a significant fraction of lignocellulosic biomass, co-utilization of lignin in addition to cellulose and hemicelluloses is vital to the economic viability of cellulosic biorefineries. The recalcitran ...
DOI:
10.1016/j.biotechadv.2019.107418
CHORUS:
10.1016/j.biotechadv.2019.107418

49. Biocatalyst engineering of Thermomyces Lanuginosus lipase adsorbed on hydrophobic supports: Modulation of enzyme properties for ethanolysis of oil in solvent-free systems

Author:
Erick Abreu Silveira; Sonia Moreno-Perez; Alessandra Basso; Simona Serban; Rita Pestana-Mamede; Paulo W. Tardioli; Cristiane S. Farinas; Natalia Castejon; Gloria Fernandez-Lorente; Javier Rocha-Martin; Jose M. Guisan
Source:
Journal of biotechnology 2019 v.289 pp. 126-134
ISSN:
0168-1656
Subject:
Thermomyces lanuginosus; adsorption; biocatalysts; carboxylic ester hydrolases; chemical bonding; engineering; esters; ethanolysis; hexane; hydrophobicity; mass transfer; porous media; sunflower oil
Abstract:
... Different immobilized biocatalysts of Thermomyces lanuginosus lipase (TLL) exhibited different properties for the ethanolysis of high oleic sunflower oil in solvent-free systems. TLL immobilized by interfacial adsorption on octadecyl (C-18) supports lost its 1,3-regioselectivity and produced more than 99% of ethyl esters. This reaction was influenced by mass-transfer limitations. TLL adsorbed on m ...
DOI:
10.1016/j.jbiotec.2018.11.014

50. Biocatalytic Oxidation of Biobased Furan Aldehydes: Comparison of Toxicity and Inhibition of Furans toward a Whole-Cell Biocatalyst

Author:
Ai-Di Cheng; Sai-Sai Shi; Yao Li; Min-Hua Zong; Ning Li
Source:
ACS sustainable chemistry & engineering 2019 v.8 no.3 pp. 1437-1444
ISSN:
2168-0485
Subject:
Escherichia coli; biocatalysts; carboxylic acids; catalytic activity; engineering; enzymes; furfural; hydrophobicity; hydroxymethylfurfural; neutralization; oxidation; pH; pharmaceutical industry; polymers; space and time; toxicity; viability
Abstract:
... Furan carboxylic acids are promising and renewable building blocks in polymer and pharmaceutical industries. In this work, biocatalytic synthesis of furan carboxylic acids was performed from biobased furan aldehydes using recombinant Escherichia coli expressing 3-succinoylsemialdehyde-pyridine dehydrogenase (SAPDH). Particularly, the inhibition and toxicity of furan aldehydes and their carboxylic ...
DOI:
10.1021/acssuschemeng.9b05621