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- Daniel J. Gibson; Nicole M. Nemeth; Hugues Beaufrère; Csaba Varga; Davor Ojkic; Anna Marom; Leonardo Susta
- Journal of veterinary diagnostic investigation 2019 v.31 no.5 pp. 719-725
- Aves polyomavirus 1; Beak and feather disease virus; DNA; Psittacid alphaherpesvirus 1; analytical specificity; animal pathogens; birds; cross reaction; detection limit; histology; histopathology; inclusion bodies; mixed infection; morbidity; mortality; nucleotide sequences; quantitative polymerase chain reaction; tissues; viruses
- ... Aves polyomavirus 1, psittacine beak and feather disease virus, and psittacid herpesvirus 1 are important pathogens of psittacine birds with the potential to cause substantial morbidity and mortality. Using publically available nucleotide sequences, we developed and validated a triplex real-time PCR (rtPCR) assay to rapidly detect these 3 viruses. The assay had high analytical sensitivity, detecti ...
- PubMed Central:
- Gisela González-Hein; Isabel Aguirre Gil; Rodolfo Sanchez; Bernardo Huaracan
- Journal of avian medicine and surgery 2019 v.33 no.2 pp. 141-149
- Aves polyomavirus 1; Beak and feather disease virus; beak; bird diseases; birds; feathers; genes; genetic variation; hosts; polymerase chain reaction; Chile
- ... Avian polyomavirus disease and psittacine beak and feather disease (PBFD) are both contagious viral diseases in psittacine birds with similar clinical manifestations and characterized by abnormal feathers. To determine the prevalence of Aves polyomavirus 1 (APyV) and beak and feather disease virus (BFDV) in captive, exotic psittacine birds in Chile, feathers from 250 psittacine birds, representing ...
- Jingjiao Ma; Ye Tian; Min Zhang; Yujie Li; Weili Wang; Fulin Tian; Yuqiang Chen; Yaxian Yan; Jianhe Sun
- Molecular and cellular probes 2019 v.43 pp. 80-83
- Aves polyomavirus 1; DNA; birds; breeding; death; feces; genes; genotype; monitoring; phylogeny; quantitative polymerase chain reaction; rapid methods; sequence analysis; China
- ... Budgerigar fledgling disease virus (BFDV) infection causes sudden death, abdominal distention, and feather abnormality in psittacine birds. In this study, we developed a TaqMan Real-time PCR assay to detect BFDV by targeting a conserved region in VP1 gene. The detection limit of the assay was 30 DNA gene copies, 1000 times more sensitive than conventional PCR. The coefficients of variation were le ...