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You searched for: Publication year rev 7981-2019 Remove constraint Publication year rev: 7981-2019 Subject term carboxylic ester hydrolases Remove constraint Subject term: carboxylic ester hydrolases
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1. 3,5-Diiodo-L-Thyronine Exerts Metabolically Favorable Effects on Visceral Adipose Tissue of Rats Receiving a High-Fat Diet

Author:
Elena Silvestri; Rosalba Senese; Federica Cioffi; Rita De Matteis; Davide Lattanzi; Assunta Lombardi; Antonia Giacco; Anna Maria Salzano; Andrea Scaloni; Michele Ceccarelli; Maria Moreno; Fernando Goglia; Antonia Lanni; Pieter de Lange
Source:
Nutrients 2019 v.11 no.2 pp. -
ISSN:
2072-6643
Subject:
adipocytes; adiposity; aerobiosis; beta oxidation; body weight; carboxylic ester hydrolases; energy expenditure; glycerol; high fat diet; lipids; lipolysis; liver; mitochondria; oxidative stress; phosphorylation; protein kinases; proteins; proteome; proteomics; rats; white adipose tissue
Abstract:
... When administered to rats receiving a high-fat diet (HFD), 3,5-diiodo-L-thyronine (3,5-T2) [at a dose of 25 μg/100 g body weight (BW)] is known to increase energy expenditure and to prevent HFD-induced adiposity. Here, we investigated which cellular and molecular processes in visceral white adipose tissue (VAT) contributed to the beneficial effect of 3,5-T2 over time (between 1 day and 4 weeks ...
DOI:
10.3390/nu11020278
PubMed:
30691227
PubMed Central:
PMC6412262

2. 8α-Hydroxypinoresinol isolated from Nardostachys jatamansi ameliorates cerulein-induced acute pancreatitis through inhibition of NF-κB activation

Author:
Ji-Won Choi; Joon Yeon Shin; Il-Joo Jo; Dong-Gu Kim; Ho-Joon Song; Chi-Su Yoon; Hyuncheol Oh; Youn-Chul Kim; Gi-Sang Bae; Sung-Joo Park
Source:
Molecular immunology 2019 v.114 pp. 620-628
ISSN:
0161-5890
Subject:
Nardostachys; amylases; blood sampling; blood serum; carboxylic ester hydrolases; enzyme activity; interleukin-6; lungs; mice; myeloperoxidase; pancreas; pancreatitis; therapeutics; transcription factor NF-kappa B; tumor necrosis factor-alpha
Abstract:
... Acute pancreatitis (AP) is a severe inflammatory condition of the pancreas, with no specific treatment available. We have previously reported that Nardostachys jatamansi (NJ) ameliorates cerulein-induced AP. However, the specific compound responsible for this inhibitory effect has not been identified. Therefore, in the present study, we focused on a single compound, 8α-hydroxypinoresinol (HP), fro ...
DOI:
10.1016/j.molimm.2019.09.002

3. Gynostemma Pentaphyllum Extract Ameliorates High-Fat Diet-Induced Obesity in C57BL/6N Mice by Upregulating SIRT1

Author:
Hyun Sook Lee; Su-Min Lim; Jae In Jung; So Mi Kim; Jae Kyoung Lee; Yoon Hee Kim; Kyu Min Cha; Tae Kyu Oh; Joo Myung Moon; Tae Young Kim; Eun Ji Kim
Source:
Nutrients 2019 v.11 no.10 pp. -
ISSN:
2072-6643
Subject:
Gynostemma pentaphyllum; adipocytes; adipogenesis; blood serum; carboxylic ester hydrolases; carnitine palmitoyltransferase; fatty-acid synthase; gene expression; ginsenosides; herbal medicines; high density lipoprotein cholesterol; high fat diet; hyperlipidemia; hypertrophy; inflammation; low density lipoprotein cholesterol; males; messenger RNA; mice; noninsulin-dependent diabetes mellitus; obesity; peroxisome proliferator-activated receptor gamma; triacylglycerols; white adipose tissue; Asia
Abstract:
... Gynostemma pentaphyllum is widely used in Asia as a herbal medicine to treat type 2 diabetes, dyslipidemia, and inflammation. Here, we investigated the anti-obesity effect and underlying mechanism of G. pentaphyllum extract (GPE) enriched in gypenoside L, gypenoside LI, and ginsenoside Rg3 and obtained using a novel extraction method. Five-week-old male C57BL/6N mice were fed a control diet (CD), ...
DOI:
10.3390/nu11102475
PubMed:
31618980
PubMed Central:
PMC6835433

4. A Core–Shell Structured Immobilized Lipase and Its Application in High-Temperature Reactions

Author:
Li Deng; Jiaojiao Tian; Juntao Xu; Fang Wang; Kaili Nie; Tianwei Tan
Source:
Applied biochemistry and biotechnology 2019 v.189 no.3 pp. 774-786
ISSN:
0273-2289
Subject:
adsorption; alkali treatment; bamboos; calcification; calcium chloride; carboxylic ester hydrolases; catalytic activity; cost effectiveness; esterification; immobilized enzymes; industrial applications; micropores; response surface methodology; scanning electron microscopy; sodium alginate; sodium hydroxide; surface roughness; thermal stability
Abstract:
... In this research, a core–shell immobilized lipase was constructed with the cost-effective bamboo carrier. Natural bamboo powder was pretreated by different methods. The results of SEM and BET demonstrated that alkali pretreatment increased the surface roughness with formed wrinkles and micropores, facilitating the lipase adsorption on the pretreated carrier. The optimal immobilization conditions w ...
DOI:
10.1007/s12010-019-03025-x

5. A Green Process for Starch Oleate Synthesis by Cryptococcus sp. MTCC 5455 Lipase and Its Potential as an Emulsifying Agent

Author:
Aarthy Mayilvahanan; Aparna Ramchary; Ayyadurai Niraikulam; Gowthaman Marichetti Kuppuswami; Kamini Numbi Ramudu
Source:
Stärke = 2019 v.71 no.1-2 pp. e1700325
ISSN:
0038-9056
Subject:
X-ray diffraction; carboxylic ester hydrolases; emulsifiers; emulsions; esterification; food industry; oleic acid; potato starch; scanning electron microscopy; starch; thermal stability; thermogravimetry; yeasts
Abstract:
... Starch oleate is synthesized in an aqueous medium using lipase from the yeast Cryptococcus sp. MTCC 5455. The optimum conditions of esterification are found at 24 h and 30 °C with an oleic acid/starch molar ratio of 1:2 using 500 U of lipase and the degree of substitution was 0.26. Spectral techniques confirm the presence of oleate group in the modified potato starch. Scanning electron microscopic ...
DOI:
10.1002/star.201700325

6. A KAS-III Heterodimer in Lipstatin Biosynthesis Nondecarboxylatively Condenses C₈ and C₁₄ Fatty Acyl-CoA Substrates by a Variable Mechanism during the Establishment of a C₂₂ Aliphatic Skeleton

Author:
Daozhong Zhang; Fang Zhang; Wen Liu
Source:
Journal of the American Chemical Society 2019 v.141 no.9 pp. 3993-4001
ISSN:
1520-5126
Subject:
Lewis bases; acyl coenzyme A; bacteria; biosynthesis; carboxylic ester hydrolases; catalytic activity; chemical bonding; condensation reactions; deprotonation; enzyme inhibitors; phylogeny; proteins; stereochemistry; stereoselectivity; thioesters
Abstract:
... β-Ketoacyl-acyl carrier protein synthase-III (KAS-III) and its homologues are thiolase-fold proteins that typically behave as homodimers functioning in diverse thioester-based reactions for C–C, C–O, or C–N bond formation. Here, we report an exception observed in the biosynthesis of lipstatin. During the establishment of the C₂₂ aliphatic skeleton of this β-lactone lipase inhibitor, LstA and LstB, ...
DOI:
10.1021/jacs.8b12843

7. A Photochemical Route to Optically Active Hexahydro-4H-furopyranol, a High-Affinity P2 Ligand for HIV-1 Protease Inhibitors

Author:
Arun K. Ghosh; William L. Robinson
Source:
Journal of organic chemistry 2019 v.84 no.15 pp. 9801-9805
ISSN:
1520-6904
Subject:
Human immunodeficiency virus 1; alcohols; carboxylic ester hydrolases; catalytic activity; enantiomers; hydrogenation; ligands; organic chemistry; photochemistry; proteinase inhibitors; stereochemistry
Abstract:
... We describe here the syntheses of optically pure (3aS,4S,7aR)-hexahydro-4H-furo[2,3-b]pyran-4-ol and (3aR,4R,7aS)-hexahydro-4H-furo[2,3-b]pyran-4-ol. These stereochemically defined heterocycles are important high-affinity P2 ligands for a variety of highly potent HIV-1 protease inhibitors. The key steps involve an efficient Paternò–Büchi [2 + 2] photocycloaddition, catalytic hydrogenation, acid- ...
DOI:
10.1021/acs.joc.9b01361
PubMed:
31310117
PubMed Central:
PMC7048240

8. A Solvent-tolerant Alkaline Lipase from Bacillus sp. DM9K3 and Its Potential Applications in Esterification and Polymer Degradation

Author:
P. Singh; V. Patel; V. Shah; D. Madamwar
Source:
Applied biochemistry and microbiology 2019 v.55 no.6 pp. 603-614
ISSN:
0003-6838
Subject:
Bacillus (bacteria); EDTA (chelating agent); acetone; benzene; biodegradation; calcium; carboxylic ester hydrolases; culture media; drug delivery systems; enzyme activity; esterification; extracellular enzymes; hypersalinity; ions; lactones; magnesium; microparticles; pH; palmitates; polymers; potassium; solvents; India
Abstract:
... Solvent-tolerant lipase produced by Bacillus sp. DM9K3 has been isolated from the hypersaline area, White Rann of Kachchh, Gujarat, India. The strain initially showed lipase activity of 11.1 U/mL in a basal medium which increased to 52.0 U/mL under optimized culture conditions. Bacillus sp. DM9K3 exhibited stability at 7% salinity, pH 9.0 and 50°C. The extracellular lipase was partially purified b ...
DOI:
10.1134/S0003683819060139

9. A comprehensive review of biodiesel production methods from various feedstocks

Author:
R. Selvaraj; R. Praveenkumar; I. Ganesh Moorthy
Source:
Biofuels 2019 v.10 no.3 pp. 325-333
ISSN:
1759-7277
Subject:
Fourier transform infrared spectroscopy; algal oils; algorithms; animal fats and oils; biocatalysts; biodiesel; carboxylic ester hydrolases; catalytic activity; cooking fats and oils; energy; fatty acid methyl esters; feedstocks; free fatty acids; fuel production; gas chromatography-mass spectrometry; magnetism; methanol; mixing; nanocatalysts; nanoparticles; neural networks; pH; response surface methodology; surveys; temperature; transesterification; transmission electron microscopy; ultrasonics; viscosity
Abstract:
... This article reviews the performance of biodiesel production from various feedstocks and analyzes the associated challenges. The existing literature survey dealt with the potential and important feedstocks like edible oil, non-edible oil, animal fat and algae oil for the biodiesel production. The result shows that the various sources have different yield due to processes variables. The yield of bi ...
DOI:
10.1080/17597269.2016.1204584

10. A differential transcriptional profile by Culex quinquefasciatus larvae resistant to Lysinibacillus sphaericus IAB59 highlights genes and pathways associated with the resistance phenotype

Author:
Tatiana Maria Teodoro Rezende; Antonio Mauro Rezende; Gabriel Luz Wallau; Crhisllane Rafaele Santos Vasconcelos; Osvaldo Pompílio de-Melo-Neto; Maria Helena Neves Lobo Silva-Filha; Tatiany Patrícia Romão
Source:
Parasites & vectors 2019 v.12 no.1 pp. 407
ISSN:
1756-3305
Subject:
Culex quinquefasciatus; DNA replication; DNA-binding proteins; Lysinibacillus sphaericus; alpha-glucosidase; apolipoprotein D; bacteria; biochemical pathways; bioinformatics; carboxylic ester hydrolases; chronic exposure; data collection; entomopathogens; gene expression regulation; genes; heat shock proteins; insect larvae; ion channels; juvenile hormones; lipid metabolism; messenger RNA; midgut; phenotype; polypeptides; reverse transcriptase polymerase chain reaction; sequence analysis; transcription (genetics); transcriptomics
Abstract:
... BACKGROUND: The study of the mechanisms by which larvae of the Culex quinquefasciatus mosquito survive exposure to the entomopathogen Lysinibacillus sphaericus has benefited substantially from the generation of laboratory-selected colonies resistant to this bacterium. One such colony, RIAB59, was selected after regular long-term exposure of larvae to the L. sphaericus IAB59 strain. This strain is ...
DOI:
10.1186/s13071-019-3661-y
PubMed:
31429782
PubMed Central:
PMC6702717

11. A fluorescence-based activity assay for immobilized lipases in non-native media

Author:
Kim N. Ingenbosch; Anna Rousek; Dennis S. Wunschik; Kerstin Hoffmann-Jacobsen
Source:
Analytical biochemistry 2019 v.569 pp. 22-27
ISSN:
0003-2697
Subject:
Bacillus subtilis; Pseudozyma antarctica; acetonitrile; biocatalysts; carboxylic ester hydrolases; enzyme activity; ethanol; fluorescence; hydrolysis; prediction; solvents
Abstract:
... A new method for the analysis of lipase activity in the immobilized state is developed. The fluorescence assay aims to quantify the potential of lipases for the application in organic solvents. As lipases are universally immobilized on polymeric carriers for the use in bioorganic synthesis, the assay includes an immobilization step on the walls of polymeric cuvettes. The activity of the immobilize ...
DOI:
10.1016/j.ab.2019.01.005

12. A genetically-encoded synthetic self-assembled multienzyme complex of lipase and P450 fatty acid decarboxylase for efficient bioproduction of fatty alkenes

Author:
Fei Li; Kaixin Yang; Yun Xu; Yangge Qiao; Yunjun Yan; Jinyong Yan
Source:
Bioresource technology 2019 v.272 pp. 451-457
ISSN:
0960-8524
Subject:
alkenes; carboxylic ester hydrolases; cellulose; decarboxylation; fatty acids; genetic engineering; hydrolysis; triacylglycerols
Abstract:
... We develop an efficient and economic cascade multienzymes for fatty alkene bioproduction based on the lipase hydrolysis coupled to the P450 decarboxylation in the form of multiple enzyme complex. One step preparation of a multienzyme complex was based on a mixture of cell extracts including dockerin-enzyme fusions and one cohesin-cellulose binding module (CBM) fusion through high specific interact ...
DOI:
10.1016/j.biortech.2018.10.067

13. A lipase-responsive antifungal nanoplatform for synergistic photodynamic/photothermal/pharmaco-therapy of azole-resistant Candida albicans infections

Author:
Dongliang Yang; Xinyi Lv; Lei Xue; Nan Yang; Yanling Hu; Lixing Weng; Nina Fu; Lianhui Wang; Xiaochen Dong
Source:
Chemical communications 2019 v.55 no.100 pp. 15145-15148
ISSN:
1364-548X
Subject:
Candida albicans; biofilm; carboxylic ester hydrolases; chemical compounds; chemical reactions; drug therapy; drugs; heat
Abstract:
... A lipase-triggered drug release nanoplatform (PGL-DPP–FLU NPs) for multi-modal antifungal therapy is developed. The lipases secreted by C. albicans can accelerate FLU release. The ROS and heat produced by PGL-DPP–FLU NPs make C. albicans more vulnerable to FLU, thereby PGL-DPP–FLU NPs exhibit high performance for combating azole-resistant C. albicans biofilms and wound infection. ...
DOI:
10.1039/c9cc08463k

14. A multi-enzyme cascade reaction for the production of 6-hydroxyhexanoic acid

Author:
Vishnu S.T. Srinivasamurthy; Dominique Böttcher; Uwe T. Bornscheuer
Source:
Zeitschrift für Naturforschung 2019 v.74 no.3-4 pp. 71-76
ISSN:
1865-7125
Subject:
Escherichia coli; NADP (coenzyme); alcohol dehydrogenase; carboxylic ester hydrolases; catalytic activity
Abstract:
... Multi-enzyme cascade reactions capture the essence of nature’s efficiency by increasing the productivity of a process. Here we describe one such three-enzyme cascade for the synthesis of 6-hydroxyhexanoic acid. Whole cells of Escherichia coli co-expressing an alcohol dehydrogenase and a Baeyer-Villiger monooxygenase (CHMO) for internal cofactor regeneration were used without the supply of external ...
DOI:
10.1515/znc-2018-0216

15. A novel lipase-catalyzed method for preparing ELR-based bioconjugates

Author:
Ana M. Testera; Mercedes Santos; Alessandra Girotti; F. Javier Arias; J. Manuel Báñez; Matilde Alonso; J. Carlos Rodríguez-Cabello
Source:
International journal of biological macromolecules 2019 v.121 pp. 752-759
ISSN:
0141-8130
Subject:
Pseudozyma antarctica; amino acids; biocompatible materials; carboxylic ester hydrolases; catalytic activity; glucose; illicit drugs; medicine; models; pH; phenylboronic acids; polyethylene glycol; porous media; solvents; temperature
Abstract:
... Herein we present a novel one-pot method for the chemical modification of elastin-like recombinamers (ELRs) in a mild and efficient manner involving enzymatic catalysis with Candida antarctica lipase B. The introduction of different functionalities into such ELRs could open up new possibilities for the development of advanced biomaterials for regenerative medicine and, specifically, for controlled ...
DOI:
10.1016/j.ijbiomac.2018.10.028

16. A novel process for biodiesel production from sludge palm oil

Author:
Papasanee Muanruksa; James Winterburn; Pakawadee Kaewkannetra
Source:
MethodsX 2019 v.6 pp. 2838-2844
ISSN:
2215-0161
Subject:
biocatalysts; biodiesel; carboxylic ester hydrolases; catalytic activity; esterification; fatty acid composition; fatty acids; free fatty acids; fuel production; glycerol; oil refining; palm oils; sludge; soaps
Abstract:
... Typically, sludge palm oil (SPO) which discharged from the palm oil refining process, is a low cost material of potential value, due to its high free fatty acid content. Accordingly, there is potential for upgrading low grade oil into valuable biofuel. In this work, we present a novel method for biodiesel production from SPO. The process consists of two steps (i) free fatty acid (FFA) extraction ( ...
DOI:
10.1016/j.mex.2019.09.039
PubMed:
31871917
PubMed Central:
PMC6911963

17. A pilot study on the nutraceutical properties of the Citrus hybrid Tacle® as a dietary source of polyphenols for supplementation in metabolic disorders

Author:
Teresa Casacchia; Maria Antonietta Occhiuzzi; Fedora Grande; Bruno Rizzuti; Maria Concetta Granieri; Carmine Rocca; Alfonsina Gattuso; Antonio Garofalo; Tommaso Angelone; Giancarlo Statti
Source:
Journal of functional foods 2019 v.52 pp. 370-381
ISSN:
1756-4646
Subject:
Citrus; active sites; antioxidant activity; carboxylic ester hydrolases; clementines; crossing; diabetes; fruits; functional foods; hesperidin; hybrids; in vivo studies; naringin; obesity; parents; polyphenols; rats; tetraploidy
Abstract:
... Tacle® is a citrus variety obtained from the crossbreeding of Clementine and Tarocco tetraploids. This new fruit recently gained an increasing interest due to its commercial value and nutraceutical properties. A high content in polyphenols confers to its extracts a protective activity against oxidative agents involved in several degenerative disorders. In this study, we investigated the antioxidan ...
DOI:
10.1016/j.jff.2018.11.030

18. A strategic approach of enzyme engineering by attribute ranking and enzyme immobilization on zinc oxide nanoparticles to attain thermostability in mesophilic Bacillus subtilis lipase for detergent formulation

Author:
Mohd Faheem Khan; Debasree Kundu; Chinmay Hazra; Sanjukta Patra
Source:
International journal of biological macromolecules 2019 v.136 pp. 66-82
ISSN:
0141-8130
Subject:
Bacillus subtilis; amino acids; analytical methods; carboxylic ester hydrolases; data collection; detergents; engineering; fabrics; glutaraldehyde; immobilized enzymes; lipid content; models; mutants; mutation; nanoparticles; oils; pH; polyethyleneimine; protein engineering; stain removal; storage quality; storage time; thermal stability; zinc oxide
Abstract:
... The present study envisaged rationalized protein engineering approach to attain thermostability in a mesophilic Bacillus subtilis lipase. Contributing amino acids for thermostability were analyzed from homologous thermophilic-mesophilic protein dataset through relative abundance and generated ranking model. Analyses divulged priority of charged amino acids for thermostability. Ranking model was us ...
DOI:
10.1016/j.ijbiomac.2019.06.042

19. A study of the digestive enzyme activities in scaleless carp (Gymnocypris przewalskii) on the Qinghai-Tibetan Plateau

Author:
Haining Tian; Yuqiong Meng; Changzhong Li; Lunxiang Zhang; Guoqian Xu; Yuan Shi; Jianquan Shi; Hongfang Qi; Rui Ma
Source:
Aquaculture reports 2019 v.13 pp. 100174
ISSN:
2352-5134
Subject:
Gymnocypris przewalskii; amylases; body weight; carbohydrates; carboxylic ester hydrolases; carp; chymotrypsin; digestion; enzyme activity; foregut; hepatopancreas; hindgut; intestines; nutrients; nutritional status; temporal variation; trypsin; China
Abstract:
... Two experiments were conducted to study the digestive enzyme activities in scaleless carp (Gymnocypris przewalskii) on the Qinghai-Tibetan Plateau. In experiment 1, the distribution of digestive enzymes in various digestive organs of G. przewalskii (body weight: 118.24 ± 9.48 g) was studied. Results showed that digestive enzyme specific activities in intestine were significantly higher than those ...
DOI:
10.1016/j.aqrep.2018.10.002

20. Acceleration of Swiss cheese ripening by microbial lipase without affecting its quality characteristics

Author:
Sapna Rani; Sharmili Jagtap
Source:
Journal of food science and technology 2019 v.56 no.1 pp. 497-506
ISSN:
0022-1155
Subject:
Swiss cheese; brittleness; carboxylic ester hydrolases; cheese ripening; fat globules; hardness; microstructure; milk; physicochemical properties; sensory properties; statistical analysis
Abstract:
... The effect of exogenous microbial lipase enzyme on the ripening of Swiss cheese (0, 200, and 800U lipase in 30 L milk) was investigated for the physico-chemical, textural and sensory properties, along with its microstructure. The aim of this study was to investigate the application of microbial lipase to accelerate the ripening without affecting its original desirable quality characteristics. The ...
DOI:
10.1007/s13197-018-3482-6
PubMed:
30728594
PubMed Central:
PMC6342820