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You searched for: Publication year rev 7981-2019 Remove constraint Publication year rev: 7981-2019 Subject term circular dichroism spectroscopy Remove constraint Subject term: circular dichroism spectroscopy
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1. 2H-Pyranone and isocoumarin derivatives isolated from the plant pathogenic fungus Leptosphaena maculans

Author:
Ming-Fang Liao; Kai Wang; Jin-Wei Ren; Li Liu; Lei Cai; Jun-Jie Han; Hong-Wei Liu
Source:
Journal of Asian natural products research 2019 v.21 no.10 pp. 939-946
ISSN:
1477-2213
Subject:
alpha-glucosidase; circular dichroism spectroscopy; electrospray ionization mass spectrometry; inhibitory concentration 50; nuclear magnetic resonance spectroscopy; plant pathogenic fungi
Abstract:
... Two new 2H-pyranones and two new isocoumarin derivatives, maculanslines A-D (1–4), together with seven known compounds (5–11), were isolated from the plant pathogenic fungus Leptosphaena maculans. Their planar structures and absolute configuration were elucidated by comprehensive spectroscopic techniques including high-resolution electrospray ionization mass spectrum, 1D and 2D nuclear magnetic re ...
DOI:
10.1080/10286020.2018.1485661

2. A Flexible Acetylcholinesterase-Modified Graphene for Chiral Pesticide Sensor

Author:
Yunpeng Zhang; Xiaotong Liu; Shi Qiu; Qiuqi Zhang; Wei Tang; Hongtao Liu; Yunlong Guo; Yongqiang Ma; Xiaojun Guo; Yunqi Liu
Source:
Journal of the American Chemical Society 2019 v.141 no.37 pp. 14643-14649
ISSN:
1520-5126
Subject:
acetylcholinesterase; biocompatibility; circular dichroism spectroscopy; enantiomers; graphene; mobile telephones; pesticides; quantum mechanics; rapid methods
Abstract:
... Sensors based on graphene are promising devices for chemical and biological detection owing to their high sensitivity, biocompatibility, and low costs. However, for chiral recognition, which is very important in biological systems, graphene sensors remain unable to discriminate enantiomers. Here, using chiral pesticide molecules as an example, we realized a highly sensitive graphene chiral sensor ...
DOI:
10.1021/jacs.9b05724

3. A Highly Luminescent Chiral Tetrahedral Eu₄L₄(L′)₄ Cage: Chirality Induction, Chirality Memory, and Circularly Polarized Luminescence

Author:
Yanyan Zhou; Hongfeng Li; Tianyu Zhu; Ting Gao; Pengfei Yan
Source:
Journal of the American Chemical Society 2019 v.141 no.50 pp. 19634-19643
ISSN:
1520-5126
Subject:
X-ray diffraction; aromatic amines; circular dichroism spectroscopy; diastereoselectivity; enantiomers; enantioselectivity; europium; ions; ligands; luminescence; nuclear magnetic resonance spectroscopy; optical isomerism
Abstract:
... Chiral lanthanide cages with circularly polarized luminescence (CPL) properties have found potential application in enantioselective guest recognition and sensing. However, it still remains a big challenge to develop a simple and robust method for the diastereoselective assembly of homochiral lanthanide cages in view of the large lability of the Ln(III) ions. Herein, we report the first example of ...
DOI:
10.1021/jacs.9b07178

4. A New Meroterpene, A New Benzofuran Derivative and Other Constituents from Cultures of the Marine Sponge-Associated Fungus Acremonium persicinum KUFA 1007 and Their Anticholinesterase Activities

Author:
Ana J. S. Alves; José A. Pereira; Tida Dethoup; Sara Cravo; Sharad Mistry; Artur M. S. Silva; Madalena M. M. Pinto; Anake Kijjoa
Source:
Marine drugs 2019 v.17 no.6 pp. -
ISSN:
1660-3397
Subject:
Acremonium; acetylcholinesterase; benzofurans; chemical derivatives; cholinesterase; circular dichroism spectroscopy; enzyme activity; enzyme inhibition; ergosterol; fungi; mass spectrometry; meroterpenoids; nuclear magnetic resonance spectroscopy
Abstract:
... Previously unreported meroterpene, acremine S (1), and benzopyran derivative, acremine T (2), were isolated, together with lumichrome (3), ergosterol (4) and ergosterol 5,8-endoperoxide, from cultures of the marine sponge-associated fungus Acremonium persicinum KUF1007. The structure of the previously unreported compounds was established based on an extensive analysis of 1D and 2D NMR spectra as w ...
DOI:
10.3390/md17060379
PubMed:
31242631
PubMed Central:
PMC6628235

5. A Novel Mini Protein Design of Haloalkane Dehalogenase

Author:
Nurul Hazwani Daud; Thean Chor Leow; Siti Nurbaya Oslan; Abu Bakar Salleh
Source:
Molecular biotechnology 2019 v.61 no.7 pp. 477-488
ISSN:
1073-6085
Subject:
binding capacity; calorimetry; circular dichroism spectroscopy; computer simulation; enzyme activity; enzymes; industrial applications; models; proteins; thermal stability; titration; toxicity
Abstract:
... The application of native enzymes may not be economical owing to the stability factor. A smaller protein molecule may be less susceptible to external stresses. Haloalkane dehalogenases (HLDs) that act on toxic haloalkanes may be incorporated as bioreceptors to detect haloalkane contaminants. Therefore, this study aims to develop mini proteins of HLD as an alternative bioreceptor which was able to ...
DOI:
10.1007/s12033-019-00169-3

6. A Structural Variant Approach for Establishing a Detection Limit in Differential Hydrogen Exchange-Mass Spectrometry Measurements

Author:
Tyler S. Hageman; David D. Weis
Source:
Analytical chemistry 2019 v.91 no.13 pp. 8017-8024
ISSN:
1520-6882
Subject:
binding proteins; circular dichroism spectroscopy; detection limit; differential scanning calorimetry; gel chromatography; hydrogen; maltose; models; mutants; protein structure; statistical analysis
Abstract:
... Hydrogen exchange-mass spectrometry (HX-MS) is widely promoted for its ability to detect subtle perturbations in protein structure, but such perturbations will result in small differences in HX. However, the detection limit of HX-MS has not been widely investigated, nor is there a useful approach for defining the detection limit of HX-MS measurements. In this work, we designed a well-characterized ...
DOI:
10.1021/acs.analchem.9b01326

7. A beetle antifreeze protein protects lactate dehydrogenase under freeze-thawing

Author:
Celeste Rodriguez; Seyed Sajjadi; Ravinder Abrol; Xin Wen
Source:
International journal of biological macromolecules 2019 v.136 pp. 1153-1160
ISSN:
0141-8130
Subject:
Coleoptera; alanine; antifreeze proteins; arginine; bovine serum albumin; circular dichroism spectroscopy; denaturation; enzyme activity; freezing; lactate dehydrogenase; models; molecular dynamics; mutants; protective effect; thawing
Abstract:
... Effects of a beetle antifreeze proteins (AFP) from Dendroides canadensis (DAFP-1) on a model freeze-labile enzyme, lactate dehydrogenase (LDH), were investigated under freezing and thawing conditions. The presence of DAFP-1 can effectively protect the enzymatic activity of LDH upon repeated freezing and thawing and the protective role of DAFP-1 is more significant than that of bovine serum albumin ...
DOI:
10.1016/j.ijbiomac.2019.06.067

8. A chiral assembly of gold nanoparticle trimer-based biosensors for ultrasensitive detection of the major allergen tropomyosin in shellfish

Author:
Yanbo Wang; Zhiheng Rao; Jinru Zhou; Lei Zheng; Linglin Fu
Source:
Biosensors & bioelectronics 2019 v.132 pp. 84-89
ISSN:
0956-5663
Subject:
adulterated products; allergens; biomarkers; biosensors; circular dichroism spectroscopy; conserved sequences; detection limit; monoclonal antibodies; nanogold; phylogeny; polymers; shellfish; tropomyosins
Abstract:
... A biosensor based on a chiral assembly of polymer of gold nanoparticle (AuNP) trimers was developed for the detection and quantification of the major shellfish allergen tropomyosin (TROP). TROP and anti-TROP monoclonal antibodies (mAb) were immobilized on 20 nm and 30 nm 16-mercaptohexadecanoic acid (16-MHDA) functionalized AuNPs to assemble a trimer, which has a Circular dichroism (CD) signal. Th ...
DOI:
10.1016/j.bios.2019.02.038

9. A close look on the effect of polyethylene glycol on the levansucrase thermostability: a case study of Brenneria sp. levansucrase

Author:
Wei Xu; Wenli Zhang; Cuie Guang; Tao Zhang; Wanmeng Mu
Source:
Journal of the science of food and agriculture 2019 v.99 no.14 pp. 6315-6323
ISSN:
0022-5142
Subject:
Brenneria; case studies; circular dichroism spectroscopy; enzyme activity; fluorescence; guidelines; hydrophobicity; levansucrase; metal ions; polyethylene glycol; protein structure; thermal stability
Abstract:
... BACKGROUND: To increase the low residual activity of levansucrase during long‐time processing, an enhancement of its weak thermostability is needed. Here, the effect of metal ions and polyethylene glycol (PEG) on the thermostability of levansucrase from Brenneria sp. EniD312 were studied and evaluated. The residual activity was determined and the protein structure was evaluated by circular dichroi ...
DOI:
10.1002/jsfa.9908

10. A comparative analysis of catalytic activity and stability of microbial transglutaminase in controlled denaturing conditions

Author:
Andrea Caporale; Alessandra Monti; Fabio Selis; Annamaria Sandomenico; Giancarlo Tonon; Menotti Ruvo; Nunzianna Doti
Source:
Journal of biotechnology 2019 v.302 pp. 48-57
ISSN:
0168-1656
Subject:
biocatalysis; chemical bonding; circular dichroism spectroscopy; comparative study; crosslinking; enzyme stability; fluorescence; guanidinium; models; pH; peptides; protein-glutamine gamma-glutamyltransferase; proteins; substrate specificity; urea
Abstract:
... Microbial transglutaminases (MTGs) catalyzes the formation of Gln-Lys isopeptide bonds and are widely used for the cross-linking of proteins and peptides in food and in biotechnological applications for bioconjugation reactions. In view of its practical utility, a comparative study of the catalytic activity and stability of the enzyme in a wide range of denaturing conditions has been performed thr ...
DOI:
10.1016/j.jbiotec.2019.06.299

11. A comprehensive strategy for studying protein-metabolite interactions by metabolomics and native mass spectrometry

Author:
Qian Qin; Bohong Wang; Jiayue Wang; Mengmeng Chang; Tian Xia; Xianzhe Shi; Guowang Xu
Source:
Talanta 2019 v.194 pp. 63-72
ISSN:
0039-9140
Subject:
arachidonic acid; beta oxidation; calorimetry; circular dichroism spectroscopy; endothermy; ligands; linoleic acid; mass spectrometry; metabolites; metabolomics; molecular models; oleic acid; palmitoleic acid; peroxisome proliferator-activated receptor gamma; spectrometers; stoichiometry; thermodynamics; titration
Abstract:
... Protein-metabolite interactions play important roles in many cellular and physiological processes in biological systems. However, the lack of effective research approaches impedes the understanding of the protein-metabolite interactions. In this study, a novel comprehensive strategy by combining metabolomics platform with native mass spectrometry was developed for investigating the protein-metabol ...
DOI:
10.1016/j.talanta.2018.10.010

12. A critical challenge in the development of antibody: Selecting the appropriate fragment of the target protein as an antigen based on various epitopes or similar structure

Author:
Mahboubeh Razzaqi; Mohammad Javad Rasaee; Maliheh Paknejad
Source:
Molecular immunology 2019 v.111 pp. 128-135
ISSN:
0161-5890
Subject:
Escherichia coli; affinity chromatography; analysis of variance; circular dichroism spectroscopy; epitopes; immune response; immunization; mice; monoclonal antibodies; neoplasms; prediction; proteins; pyruvate kinase; vaccines
Abstract:
... The main challenge in the development of antibody is to select the appropriate antigen particularly when a truncated protein is used for immunization or as vaccine antigen. In previous studies, fragment selection was mainly based on epitopes and less often on the structure. Fewer studies have paid attention to the prediction of the truncated protein 3D structure and retained its similarity in the ...
DOI:
10.1016/j.molimm.2019.04.018

13. A duplex connection can further illuminate G-quadruplex/crystal violet complex

Author:
Shuang Wang; Jiahui Zhao; Shasha Lu; Jian Sun; Xiurong Yang
Source:
Chemical communications 2019 v.55 no.13 pp. 1911-1914
ISSN:
1364-548X
Subject:
chemical compounds; chemical reactions; circular dichroism spectroscopy; density functional theory; fluorescence; fluorescence emission spectroscopy; gentian violet; nucleic acids
Abstract:
... Duplex-connected G-quadruplex (dsG4) has a more evident promotion of the fluorescent emission of crystal violet than pure G-quadruplex (G4), which is firstly found and investigated systematically using fluorescence spectra, circular dichroism, and density functional theory. This new phenomenon also shows potential in application for target nucleic acid detection. ...
DOI:
10.1039/c8cc09940e

14. A molecular investigation into the interaction of SiO2 nanoparticles with elastase by multispectroscopic techniques and kinetic studies

Author:
Sakineh Sadeghi-kaji; Behzad Shareghi; Ali Akbar Saboury; Sadegh Farhadian; Roohullah Hemmati
Source:
International journal of biological macromolecules 2019 v.134 pp. 216-222
ISSN:
0141-8130
Subject:
buffers; circular dichroism spectroscopy; denaturation; elastase; enzyme activity; fluorescence; fluorescence emission spectroscopy; hydrogen bonding; industry; melting point; nanoparticles; pH; silica; surveys; temperature; thermal stability; ultraviolet-visible spectroscopy; van der Waals forces
Abstract:
... The increasing use of nanoparticles in various industries has triggered the need to study their interconnection with biological macromolecules. The goal of this study was to survey the SiO2-nanoparticles efficacy on the thermal denaturation, conformation and activity of elastase at three temperatures of 303, 313 and 323K in the Tris buffer at pH of 8.5. In this work, distinct techniques such as UV ...
DOI:
10.1016/j.ijbiomac.2019.05.007

15. A multi-channel in situ light scattering instrument utilized for monitoring protein aggregation and liquid dense cluster formation

Author:
Sven Falke; Hévila Brognaro; Arayik Martirosyan; Karsten Dierks; Christian Betzel
Source:
Heliyon 2019 v.5 no.12 pp. e03016
ISSN:
2405-8440
Subject:
biotechnology; cattle; circular dichroism spectroscopy; glucose; interdisciplinary research; isomerases; liquids; materials science; separation; trypsin inhibitors; ultraviolet radiation
Abstract:
... Liquid-liquid phase separation (LLPS) phenomena have been observed in vitro as well as in vivo and came in focus of interdisciplinary research activities particularly aiming at understanding the physico-chemical pathways of LLPS and its functionality in recent years. Dynamic light scattering (DLS) has been proven to be a most efficient method to analyze macromolecular clustering in solutions and s ...
DOI:
10.1016/j.heliyon.2019.e03016

16. A natural inhibitor from Alisma orientale against human carboxylesterase 2: Kinetics, circular dichroism spectroscopic analysis, and docking simulation

Author:
Jing Yi; Rong Bai; Yue An; Tian-Tian Liu; Jia-Hao Liang; Xiang-Ge Tian; Xiao-Kui Huo; Lei Feng; Jing Ning; Cheng-Peng Sun; Xiao-Chi Ma; Hou-Li Zhang
Source:
International journal of biological macromolecules 2019 v.133 pp. 184-189
ISSN:
0141-8130
Subject:
Alisma orientale; Oriental traditional medicine; carboxylesterase; circular dichroism spectroscopy; computer simulation; humans; hydrolysis; inhibitory concentration 50; nuclear magnetic resonance spectroscopy; spectral analysis; triterpenoids
Abstract:
... As a part of our searching for natural human carboxylesterase 2 (human CES 2) inhibitors from traditional Chinese medicine, we found that the extract of Alisma orientale significantly inhibited human CES 2 in vitro. The investigation on A. orientale led to the isolation of a new protostane-type triterpenoid alismanin I (1). Its structure was determined according to HRESIMS, 1D and 2D NMR spectra. ...
DOI:
10.1016/j.ijbiomac.2019.04.099

17. A new dual-peptide strategy for enhancing antioxidant activity and exploring the enhancement mechanism

Author:
Cong Ma; Na Sun; Simin Zhang; Jingru Zheng; Songyi Lin
Source:
Food & function 2019 v.10 no.11 pp. 7533-7543
ISSN:
2042-650X
Subject:
2,2-diphenyl-1-picrylhydrazyl; Holothuroidea; animal proteins; antioxidant activity; circular dichroism spectroscopy; free radical scavengers; nuclear magnetic resonance spectroscopy; peptides
Abstract:
... The development of a new generation antioxidant peptides with high free radical scavenging rates is highly desirable, but remains a great challenge. Herein, a new dual-peptide development strategy based on plant and animal proteins has been proposed to enhance the antioxidant activity of peptides. Sea cucumber peptide and pine nut peptide were selected to assemble the dual-peptides, namely, VTPYKD ...
DOI:
10.1039/c9fo01858a

18. A new functional protein‑polysaccharide conjugate based on protein concentrate from sesame processing by-products: Functional and physico-chemical properties

Author:
Aida Saatchi; Hossein Kiani; Mohsen Labbafi
Source:
International journal of biological macromolecules 2019 v.122 pp. 659-666
ISSN:
0141-8130
Subject:
byproducts; circular dichroism spectroscopy; emulsifying; emulsions; functional properties; gel electrophoresis; hulling; ingredients; maltodextrins; nutritive value; oils; pH; protein concentrates; solubility
Abstract:
... Ardeh or Tahin residue, a by-product from oil extraction and processing of dehulled, roasted and milled sesame, contains a high amount of proteins. In this research, the protein was extracted as sesame protein concentrate (SPC) and its physicochemical and functional properties were investigated. In order to improve the functional properties of sesame protein concentrate, SPC-maltodextrin conjugate ...
DOI:
10.1016/j.ijbiomac.2018.10.122

19. A new interpretation of the structure and solvent dependence of the far UV circular dichroism spectrum of short oligopeptides

Author:
Anshuman Kumar; Reinhard Schweitzer-Stenner; Bryan M. Wong
Source:
Chemical communications 2019 v.55 no.40 pp. 5701-5704
ISSN:
1364-548X
Subject:
chemical compounds; chemical reactions; circular dichroism spectroscopy; density functional theory; energy; polypeptides; proteins; solvents; tripeptides
Abstract:
... UV circular dichroism (UVCD) spectroscopy is a prominent tool for exploring secondary structures of polypeptides and proteins. In the unfolded state of these biomolecules, most of the individual residues primarily sample a conformation called polyproline II. Its CD spectrum contains a negatively biased positive couplet with a pronounced negative maximum below and a weak positive maximum above 200 ...
DOI:
10.1039/c9cc01513b

20. A new phenylpropanoid glycoside from the bark of Streblus ilicifolius (Vidal) Corner

Author:
Gaorong Zhang; Lili Hao; Dexiong Zhou; Wei Liu; Chenguo Li; Shanshan Su; Xia Xu; Xishan Huang; Jun Li
Source:
Biochemical systematics and ecology 2019 v.87 pp. 103962
ISSN:
0305-1978
Subject:
Streblus; bark; chemotaxonomy; circular dichroism spectroscopy; glycosides; lignans; nuclear magnetic resonance spectroscopy; spectral analysis
Abstract:
... A new compound, pheglycoside A (1), along with four known aromatic glycosides (2-5) and three known lignan glycosides (6–8) were isolated from Streblus ilicifolius (Vidal) Corner. The structure of compound 1 was determined by spectral analyses, including HRESIMS, 1D, and 2D NMR (COSY, HSQC, and HMBC) experiments. The absolute configuration of compound 1 was determined using the CD spectrum and exp ...
DOI:
10.1016/j.bse.2019.103962

21. A new pregnane steroid from cultures of Aspergillus versicolor

Author:
Jian-Hai Ding; Zhang-Gui Ding; Jiang-Yuan Zhao; Ming-Gang Li; Dong-Bao Hu; Xian-Jun Jiang; Dong-Sheng Zhou; Fei Wang; Meng-Liang Wen
Source:
Natural product research 2019 v.33 no.13 pp. 1885-1890
ISSN:
1478-6427
Subject:
Aspergillus versicolor; circular dichroism spectroscopy; fungi; pregnanes; quantum mechanics; spectral analysis; tin
Abstract:
... A new pregnane steroid, named aspergillon A (1), together with two known compounds, (22E,24R)-ergosta-5,7,22-trien-3β-ol (2) and (22E, 24R)-ergosta-4,6,8(14),22-tetraen-3-one (3) were isolated from cultures of the tin mine tailings-associated fungus Aspergillus versicolor. The new structure and absolute configuration were determined with the help of extensive spectroscopic analyses and quantum che ...
DOI:
10.1080/14786419.2018.1478828

22. A new tricyclic abietane diterpenoid from Salvia chloroleuca and evaluation of cytotoxic and apoptotic activities

Author:
Abolfazl Shakeri; Samira Delavari; Samad Nejad Ebrahimi; Javad Asili; Seyed Ahmad Emami; Zahra Tayarani-Najaran
Source:
Revista Brasileira de Farmacognosia 2019 v.29 no.1 pp. 30-35
ISSN:
0102-695X
Subject:
Salvia; apoptosis; breast neoplasms; butanol; circular dichroism spectroscopy; cytotoxicity; diterpenoids; enzymes; ethyl acetate; flow cytometry; human cell lines; humans; inhibitory concentration 50; methanol; methylene chloride; neoplasm cells; petroleum; prostatic neoplasms; roots
Abstract:
... In this study, sahandone (1) and a new diterpenoid named sahandol II (2) were isolated from the roots of Salvia chloroleuca Rech. f. & Aellen, Lamiaceae. The absolute configurations of compounds 1 and 2 were assigned by comparison of experimental electronic circular dichroism spectra and comparing with published data. Cytotoxic and apoptotic evaluation of the isolated compounds and the methanol cr ...
DOI:
10.1016/j.bjp.2018.09.007

23. A possible mechanism for enhancing the antioxidant activity by pulsed electric field on pine nut peptide Glutamine‐Tryptophan‐Phenylalanine‐Histidine

Author:
Sitian Zhang; Mingdi Zhang; Jie Xing; Songyi Lin
Source:
Journal of food biochemistry 2019 v.43 no.3 pp. e12714
ISSN:
0145-8884
Subject:
Pinus koraiensis; amino acids; antioxidants; bioactive compounds; chronic diseases; circular dichroism spectroscopy; drugs; fluorescence emission spectroscopy; food composition; food industry; functional foods; human health; hydrogen; nuclear magnetic resonance spectroscopy; oxygen radical absorbance capacity; peptides; pulsed electric fields; reversed-phase high performance liquid chromatography; solvents; ultraviolet-visible spectroscopy
Abstract:
... The aim of this study was to investigate the possible mechanism for increasing the antioxidant activity on peptide Glutamine‐Tryptophan‐Phenylalanine‐Histidine (QWFH) from pine nut (Pinus koraiensis) protein by a pulsed electric field (PEF). The antioxidant capacity of PEF‐treated QWFH increased significantly (p < 0.05) through 1,1‐diphenyl‐2‐pycryl‐hydrazyl radical scavenging and oxygen radical a ...
DOI:
10.1111/jfbc.12714

24. A review on methodologies for extraction, identification and quantification of allergenic proteins in prawns

Author:
M. Faisal; T. Vasiljevic; O.N. Donkor
Source:
Food research international 2019 v.121 pp. 307-318
ISSN:
0963-9969
Subject:
allergenicity; allergens; circular dichroism spectroscopy; enzyme-linked immunosorbent assay; high performance liquid chromatography; hypersensitivity; immunoblotting; polyacrylamide gel electrophoresis; shrimp
Abstract:
... Prawn allergy is one of the most common food-borne allergies and current prevention is by avoidance. This review paper summarised different methodologies for the extraction, identification and quantification of prawn protein allergens, reported in various research studies. Following extraction, allergenic components have been analysed using well-established methodologies, such as SDS-PAGE, Immunob ...
DOI:
10.1016/j.foodres.2019.03.040

25. A strategy to reduce the dose of multichiral agricultural chemicals: The herbicidal activity of metolachlor against Echinochloa crusgalli

Author:
Lu Zhao; Yue Gao; Jingqian Xie; Qiong Zhang; Fangjie Guo; Shuren Liu; Weiping Liu
Source:
Science of the total environment 2019 v.690 pp. 181-188
ISSN:
0048-9697
Subject:
Echinochloa crus-galli; biosynthesis; carbon; circular dichroism spectroscopy; enantiomers; enantioselectivity; enzymes; gibberellic acid; herbicidal properties; high performance liquid chromatography; laboratory techniques; metolachlor; tandem mass spectrometry; weeds
Abstract:
... Agricultural chemicals are normally used as mixtures of several isomers, e.g., enantiomers. In theory, in order to minimize the pesticides dose, it is desirable to use the most target-active isomer. Metolachlor is a typical multichiral herbicide belonging to amide herbicides. An asymmetric carbon atom and a chiral axis yield four stereoisomers. In this study, a novel laboratory method was develope ...
DOI:
10.1016/j.scitotenv.2019.06.521

26. A unique acyclic sesquiterpene-flavanone adduct and a new cyclic diarylheptanoid from Alpinia katsumadai

Author:
Chang-Shui Yang; Hou-Li Jiang; Heng-Yuan Li; Xiao-Yun Dong
Source:
Phytochemistry letters 2019 v.30 pp. 190-193
ISSN:
1874-3900
Subject:
Alpinia hainanensis; circular dichroism spectroscopy; nuclear magnetic resonance spectroscopy; seeds
Abstract:
... Two new compounds, sumadain D (1), and (3S,5S,6R,7S)-5,6-dihydroxy-1,7-diphenyl-methylcentrolobine (2), together with three known compounds, (lR,4S, 6R)1,4-dihydroxy-menthene (3), rhodomollein XI (4) and β-daucosterol (5), were isolated from the seeds of Alpinia katsumadai. The structures of compounds 1 and 2 were elucidated on the basis of spectroscopic techniques (UV, IR, MS, and 1D and 2D NMR), ...
DOI:
10.1016/j.phytol.2019.02.022

27. Absence of Ferromagnetism in VSe₂ Caused by Its Charge Density Wave Phase

Author:
Adolfo O. Fumega; M. Gobbi; P. Dreher; W. Wan; C. González-Orellana; M. Peña-Díaz; C. Rogero; J. Herrero-Martín; P. Gargiani; M. Ilyn; M. M. Ugeda; Victor Pardo; S. Blanco-Canosa
Source:
Journal of physical chemistry 2019 v.123 no.45 pp. 27802-27810
ISSN:
1932-7455
Subject:
X-radiation; circular dichroism spectroscopy; crystals; energy; ferromagnetism; magnetic properties; physical chemistry; stoichiometry
Abstract:
... How magnetism emerges in low-dimensional materials such as transition metal dichalcogenides at the monolayer limit is still an open question. Herein, we present a comprehensive study of the magnetic properties of single-crystal and monolayer VSe₂, both experimentally and ab initio. Magnetometry, X-ray magnetic circular dichrosim (XMCD), and ab initio calculations demonstrate that the charge densit ...
DOI:
10.1021/acs.jpcc.9b08868

28. Absolute Configuration of Bioactive Azaphilones from the Marine-Derived Fungus Pleosporales sp. CF09-1

Author:
Fei Cao; Zhi-Hui Meng; Xing Mu; Yu-Fei Yue; Hua-Jie Zhu
Source:
Journal of natural products 2019 v.82 no.2 pp. 386-392
ISSN:
1520-6025
Subject:
Alternaria brassicicola; Botryosphaeria dothidea; Fusarium oxysporum; Vibrio anguillarum; Vibrio parahaemolyticus; antifungal properties; circular dichroism spectroscopy; fungi; ketoconazole; minimum inhibitory concentration; nuclear magnetic resonance spectroscopy
Abstract:
... Investigation of the marine-derived fungus Pleosporales sp. CF09-1 cultured in modified PDB medium led to the isolation of six new azaphilone derivatives, pleosporalones B and C (1 and 2) and pleosporalones E–H (4–7), and one known analogue (3). The absolute configurations of C-2′ and C-3′ in 3 were assigned by a vibrational circular dichroism method. The C-11 relative configurations for the pair ...
DOI:
10.1021/acs.jnatprod.8b01030

29. Absolute Configurations of Synthetic Molecular Scaffolds from Vibrational CD Spectroscopy

Author:
Christian Merten; Tino P. Golub; Nora M. Kreienborg
Source:
Journal of organic chemistry 2019 v.84 no.14 pp. 8797-8814
ISSN:
1520-6904
Subject:
catalysts; chromatography; circular dichroism spectroscopy; crystals; derivatization; drugs; enantiomers; organic chemistry; organic compounds; stereoselective synthesis
Abstract:
... Vibrational circular dichroism (VCD) spectroscopy is one of the most powerful techniques for the determination of absolute configurations (AC), as it does not require any specific UV/vis chromophores, no chemical derivatization, and no growth of suitable crystals. In the past decade, it has become increasingly recognized by chemists from various fields of synthetic chemistry such as total synthesi ...
DOI:
10.1021/acs.joc.9b00466

30. Activation and conformational changes of chitinase induced by ultrasound

Author:
Furong Hou; Xiaobin Ma; Lihua Fan; Danli Wang; Wenjun Wang; Tian Ding; Xingqian Ye; Donghong Liu
Source:
Food chemistry 2019 pp. -
ISSN:
0308-8146
Subject:
Fourier transform infrared spectroscopy; Raman spectroscopy; chitinase; circular dichroism spectroscopy; enzyme activity; fluorescence emission spectroscopy; food industry; metal ions; sonication; tryptophan
Abstract:
... This study investigated the effect of ultrasound on chitinase activity and conformational changes. Results revealed that ultrasound activated chitinase with a maximum enhancement of 19.17% compared with the untreated chitinase. Furthermore, an increase of Vmax and a decrease of Km after sonication were obtained, illustrating that the affinity between chitinase and substrate was intensified. No obv ...
DOI:
10.1016/j.foodchem.2019.01.180

31. Activation of catalase via co-administration of aspirin and pioglitazone: Experimental and MLSD simulation approaches

Author:
Yunes Panahi; Reza Yekta; Gholamreza Dehghan; Samaneh Rashtbari; Nematollah Jonaidi Jafari; Ali A. Moosavi-Movahedi
Source:
Biochimie 2019 v.156 pp. 100-108
ISSN:
0300-9084
Subject:
aspirin; binding capacity; blood glucose; cardiovascular diseases; catalase; circular dichroism spectroscopy; dialysis; enzyme activity; ligands; liver; patients
Abstract:
... Aspirin (ASP) and pioglitazone (PGL) are the most common drugs that are widely used by diabetic patients to control the blood sugar and hinder cardiovascular diseases. The interaction between PGL and ASP is one of the important medical issues to clarify the safety of co-administration of these drugs. In the present study, the effect of co-administered ASP with PGL was investigated on the structure ...
DOI:
10.1016/j.biochi.2018.10.007

32. Active Control and Biosensing Application of Induced Chirality between Symmetric Metal and Graphene Nanostructures

Author:
Yongkai Wang; Zhongyu Wang; Qijing Wang; Shangqi Zhou; Qingyan Han; Wei Gao; Kaili Ren; Jianxia Qi; Jun Dong
Source:
Journal of physical chemistry 2019 v.123 no.40 pp. 24754-24762
ISSN:
1932-7455
Subject:
circular dichroism spectroscopy; graphene; magnetism; models; nanomaterials; optical isomerism; physical chemistry; wavelengths
Abstract:
... Induced circular dichroism (ICD) is found between plasmonic nanostructures and chiral molecules, where at least one of them is chiral. However, it is a great challenge to generate ICD only through achiral structures with the simple coupling model. Here, we demonstrate that ICD is approximately contributed by the cross-electromagnetic coupling between equivalent electric dipole moments and magnetic ...
DOI:
10.1021/acs.jpcc.9b07576

33. Active-site directed peptide l-Phe-d-His-l-Leu inhibits angiotensin converting enzyme activity and dexamethasone-induced hypertension in rats

Author:
Mysuru Natarajan Savitha; Jalahalli Mariswamy Siddesha; Kanve Nagaraj Suvilesh; Manjunath Yariswamy; Hamse Kameshwar Vivek; Cletus J.M. D’Souza; Muddegowda Umashankar; Bannikuppe Sannanaik Vishwanath
Source:
Peptides 2019 v.112 pp. 34-42
ISSN:
0196-9781
Subject:
active sites; animal models; antihypertensive agents; antihypertensive effect; blood pressure; cerebrovascular disorders; circular dichroism spectroscopy; collagen; computer simulation; dexamethasone; dose response; enzyme activity; fibrosis; fluorescence emission spectroscopy; heart; histidine; hypertension; inhibitory concentration 50; kidneys; peptidyl-dipeptidase A; rats; synthetic peptides; tripeptides
Abstract:
... Hypertension is the fundamental cause of cardiovascular and cerebrovascular disorders. Several natural and synthetic peptides are being used as antihypertensive agents, which target angiotensin converting enzyme (ACE), the master regulator of angiotensin (Ang) II production. In this study, we have evaluated ACE-inhibitory potential of the tripeptide l-Phenylalanyl-d-Histidyl-l-Leucine (l-Phe-d-His ...
DOI:
10.1016/j.peptides.2018.11.002

34. Adsorption and Conformation of Bovine Serum Albumin with Blue-Emitting Gold Nanoclusters at the Air/Water and Lipid/Water Interfaces

Author:
Ridwan P. Putra; Yoshinori Ikumura; Hideyuki Horino; Akiko Hori; Izabela I. Rzeznicka
Source:
Langmuir 2019 v.35 no.50 pp. 16576-16582
ISSN:
1520-5827
Subject:
adsorption; air; bovine serum albumin; circular dichroism spectroscopy; electrostatic interactions; encapsulation; gold; hydrodynamics; hydrophobic bonding; image analysis; lipids; mole fraction; nanogold; nanoparticles; oligomerization; pH; particle size; protein structure; storage time
Abstract:
... Protein-encapsulated nanoclusters (NCs) are emerging as a versatile platform for in-vivo imaging and other biomedical applications due to their ultrasmall size and excitation in the near-infrared region. Encapsulation may however affect protein structure, size, charge, and its interaction with lipid membranes. In this study, bulk characterization methods along with surface-sensitive vibrational su ...
DOI:
10.1021/acs.langmuir.9b02831

35. Aequorin as a sensitive and selective reporter for detection of dopamine: A photoprotein inhibition assay approach

Author:
Hossein Rahmani; Reza H. Sajedi
Source:
International journal of biological macromolecules 2019 v.122 pp. 677-683
ISSN:
0141-8130
Subject:
aequorin; bioluminescence; blood serum; circular dichroism spectroscopy; dopamine; drugs; fluorescence emission spectroscopy; humans; metabolites; monitoring; therapeutics; urine
Abstract:
... Dopamine is a metabolite that plays a key role in the human body and in biomedical and diagnostic applications. Thus, the concentration of this analyte has been considered in various diseases in therapeutic drug monitoring (TDM). In the present study, for the first time, a photoprotein inhibition assay strategy was developed by utilizing aequorin for the direct detection of dopamine as a receptor ...
DOI:
10.1016/j.ijbiomac.2018.10.221

36. Aggreganoids A–F, Carbon-Bridged Sesquiterpenoid Dimers and Trimers from Lindera aggregata

Author:
Xin Liu; Ji Yang; Jing Fu; Xiao-Jun Yao; Jing-Rong Wang; Liang Liu; Zhi-Hong Jiang; Guo-Yuan Zhu
Source:
Organic letters 2019 v.21 no.14 pp. 5753-5756
ISSN:
1523-7052
Subject:
Lindera; biochemical pathways; chemical reactions; chemical structure; circular dichroism spectroscopy; sesquiterpenoids
Abstract:
... Six oligomeric sesquiterpenoids, aggreganoids A–F (1–6), were isolated from Lindera aggregata. Aggreganoid A (1) and B (2) are two unprecedented methine- or methylene-bridged sesquiterpenoid trimers possessing a unique C₄₆ skeleton. Aggreganoids C–F (3–6) are the first examples of carbon-bridged disesquiterpenoids with a C₃₃ or C₃₁ skeleton in the plant kingdom. Their structures and absolute confi ...
DOI:
10.1021/acs.orglett.9b02166

37. Agreement between Experimental and Simulated Circular Dichroic Spectra of a Positively Charged Peptide in Aqueous Solution and on Self-Assembled Monolayers

Author:
Jeremy T. First; Lauren J. Webb
Source:
Journal of physical chemistry 2019 v.123 no.21 pp. 4512-4526
ISSN:
1520-5207
Subject:
aqueous solutions; bioengineering; biophysics; circular dichroism spectroscopy; databases; molecular dynamics; peptides; protein structure; proteins; solvents
Abstract:
... Successfully immobilizing functional proteins on inorganic surfaces has long been a challenge to the biophysics and bioengineering communities. This is due, in part, to a lack of understanding of the effect of nonaqueous environments on protein structure from both experimental and computational perspectives. Because most experimental information about protein structure comes from the Protein Data ...
DOI:
10.1021/acs.jpcb.9b02102

38. Alanine Scanning Studies of the Antimicrobial Peptide Aurein 1.2

Author:
Dorian Migoń; Maciej Jaśkiewicz; Damian Neubauer; Marta Bauer; Emilia Sikorska; Elżbieta Kamysz; Wojciech Kamysz
Source:
Probiotics and antimicrobial proteins 2019 v.11 no.3 pp. 1042-1054
ISSN:
1867-1306
Subject:
alanine; antimicrobial peptides; biofilm; blood serum; circular dichroism spectroscopy; frogs; hemolysis; hydrophobicity; medicinal properties; minimum inhibitory concentration; pathogens; reversed-phase liquid chromatography; secretion; structure-activity relationships
Abstract:
... Antimicrobial peptides (AMPs) are compounds widely distributed in nature that display activity against a broad spectrum of pathogens. Amphibian skin, as an organ rich in pharmacologically active peptides, appears to be an interesting source of novel AMPs. Aurein 1.2 (GLFDIIKKIAESF-NH₂) is a short 13-residue antimicrobial peptide primarily isolated from the skin secretions of Australian bell frogs. ...
DOI:
10.1007/s12602-018-9501-0
PubMed:
30569430
PubMed Central:
PMC6695355

39. Alashanoids K-M, bioactive eremophilane sesquiterpenoids from Syringa pinnatifolia

Author:
Bing Peng; Rui-Fei Zhang; Bing-Zhao Du; Shun-Gang Jiao; Xiao-Chun Zhou; Xiao-Li Gao; Su-Yi-Le Chen; Peng-Fei Tu; Xing-Yun Chai
Source:
Journal of Asian natural products research 2019 v.21 no.12 pp. 1161-1169
ISSN:
1477-2213
Subject:
Syringa; X-ray diffraction; circular dichroism spectroscopy; cytotoxicity; human cell lines; inhibitory concentration 50; macrophages; nitric oxide; nuclear magnetic resonance spectroscopy; sesquiterpenoids; stems
Abstract:
... Three new eremophilane-type sesquiterpenoids, alashanoids K–M (1–3), and one known analogue (4) were isolated from the peeled stems of Syringa pinnatifolia. All the compounds were isolated from the genus Syringa for the first time. Structures of these compounds were established using 1D and 2D NMR and MS data. The absolute configurations were determined by experimental and calculated electronic ci ...
DOI:
10.1080/10286020.2019.1694516

40. Albumin nanoparticles conjugates binding with glycan - A strategic approach for targeted drug delivery

Author:
Neha Kumari; V.L. Mathe; P.M. Dongre
Source:
International journal of biological macromolecules 2019 v.126 pp. 74-90
ISSN:
0141-8130
Subject:
Raman spectroscopy; binding sites; bovine serum albumin; cell membranes; circular dichroism spectroscopy; drugs; endothelial cells; fluorescence emission spectroscopy; fucose; galactose; glucosamine; glycoproteins; hydrodynamics; mannose; models; nanoparticles; physiological transport; polysaccharides; zeta potential
Abstract:
... Understanding the transcytosis phenomenon through albumin binding receptor (glycoproteins) is an important step towards targeted drug delivery research. The transcytosis of bovine serum albumin (BSA) occurs through its uptake by binding to glycoprotein (gp60) present on the endothelial cell membrane. gp60 possess abundant glycans like N-acetylneuraminic acid (sialic acid, NANA), l-fucose (FUC), N- ...
DOI:
10.1016/j.ijbiomac.2018.12.184

41. Alkali induced unique partially folded state of bovine serum albumin: qualitative and quantitative insights

Author:
P. Prasanthan; N. Kishore
Source:
International journal of biological macromolecules 2019 v.138 pp. 252-261
ISSN:
0141-8130
Subject:
bovine serum albumin; calorimetry; circular dichroism spectroscopy; dyes; fluorescence; pH; protein folding; titration
Abstract:
... Qualitative and quantitative understanding of partially folded states of protein is essential in gaining deeper insights into folding pathways. We have observed a partially folded state of bovine serum albumin (BSA) in the presence of 2,2,2-trifluoroethanol at pH11.2 which does not resembles the properties of the molten globule state. ThT, a frequently used marker for protein fibrils have two orde ...
DOI:
10.1016/j.ijbiomac.2019.07.082

42. Alkaloids from the Branches and Leaves of Elaeocarpus angustifolius

Author:
Wei Hong; Yu Zhang; Jun Yang; Meng-Yuan Xia; Ji-Feng Luo; Xiao-Nian Li; Yue-Hu Wang; Jun-Song Wang
Source:
Journal of natural products 2019 v.82 no.12 pp. 3221-3226
ISSN:
1520-6025
Subject:
Elaeocarpus sphaericus; alkaloids; branches; cholinesterase; circular dichroism spectroscopy; enzyme inhibition; inhibitory concentration 50; leaves; nuclear magnetic resonance spectroscopy
Abstract:
... Nine new alkaloids, (+)-1, (−)-1, 2, (+)-3, (−)-3, and 4–7, along with five known compounds (8–12), were obtained from the branches and leaves of Elaeocarpus angustifolius. The alkaloids were structurally characterized by NMR and MS data. The absolute configurations of (+)-1, (−)-1, (+)-3, and (−)-3 were determined by comparing their experimental and computed electronic circular dichroism spectra. ...
DOI:
10.1021/acs.jnatprod.8b01027

43. Alkaloids isolated from Tabernaemontana bufalina display xanthine oxidase inhibitory activity

Author:
Bao-Bao Shi; Jing Chen; Mei-Fen Bao; Ying Zeng; Xiang-Hai Cai
Source:
Phytochemistry 2019 v.166 pp. 112060
ISSN:
0031-9422
Subject:
Tabernaemontana; X-ray diffraction; aerial parts; allopurinol; circular dichroism spectroscopy; enzyme inhibition; gout; inhibitory concentration 50; isoquinoline alkaloids; monoterpenoids; nuclear magnetic resonance spectroscopy; spectral analysis; xanthine oxidase
Abstract:
... Continued interest in bioactive alkaloids led to the isolation of four undescribed alkaloids along with 74 known ones from the aerial parts of Tabernaemontana bufalina Lour. The structures of the yet undescribed alkaloids were elucidated based on NMR, IR, UV, MS and CD spectroscopic data and X-ray crystal diffraction and, according to the plant source, named as taberhaines A–D (1–4). The known com ...
DOI:
10.1016/j.phytochem.2019.112060

44. Allura red rapidly induces amyloid-like fibril formation in hen egg white lysozyme at physiological pH

Author:
Nasser Abdulatif Al-Shabib; Javed Masood Khan; Ajamaluddin Malik; Priyankar Sen; Sriroopreddy Ramireddy; Sudandiradoss Chinnappan; Salman Freeh Alamery; Fohad Mabood Husain; Aqeel Ahmad; Hani Choudhry; Mohmmad Imran Khan; Sayed Ali Shahzad
Source:
International journal of biological macromolecules 2019 v.127 pp. 297-305
ISSN:
0141-8130
Subject:
amyloid; azo dyes; circular dichroism spectroscopy; egg albumen; electrostatic interactions; fluorescence; food industry; hens; hydrogen bonding; hydrophobic bonding; light scattering; lysozyme; molecular dynamics; pH; risk; simulation models; transmission electron microscopy; turbidity
Abstract:
... Allura red (AR) is an artificial azo dye mostly used in food industries and has potential health risks. We examined the role of AR in amyloidogenesis using hen egg white lysozyme (HEWL) at pH 7.0. The amyloidogenic induction properties of AR in HEWL were identified by circular dichroism (CD), turbidity, intrinsic fluorescence, light scattering, transmission electron microscopy (TEM), and molecular ...
DOI:
10.1016/j.ijbiomac.2019.01.049

45. Alterbrassinoids A–D: Fusicoccane-Derived Diterpenoid Dimers Featuring Different Carbon Skeletons from Alternaria brassicicola

Author:
Fengli Li; Shuang Lin; Sitian Zhang; Xincai Hao; Xiao-Nian Li; Beiye Yang; Junjun Liu; Jianping Wang; Zhengxi Hu; Yonghui Zhang
Source:
Organic letters 2019 v.21 no.20 pp. 8353-8357
ISSN:
1523-7052
Subject:
Alternaria brassicicola; X-ray diffraction; anhydrides; bioactive properties; biochemical pathways; carbon; chemical reactions; chemical structure; circular dichroism spectroscopy; diterpenoids
Abstract:
... Alterbrassinoids A–D (1–4), the first examples of fusicoccane-derived diterpenoid dimers furnished by forming an undescribed C-12–C-18′ linkage, were isolated from modified cultures of Alternaria brassicicola. Compounds 1 and 2 represent unprecedented heterodimers, whereas 3 and 4 represent unprecedented homodimers, and 4 also features an undescribed anhydride motif. Their structures were assigned ...
DOI:
10.1021/acs.orglett.9b03133

46. Altering the substrate specificity of a myofibril‐bound serine proteinase from Crucian carp by site‐directed mutagenesis

Author:
Ting Li; Meng‐Si Li; Xue‐Jiao Mei; Le‐Chang Sun; Hong Liu; Ling‐Jing Zhang; Min‐Jie Cao; Guang‐Ming Liu
Source:
Journal of chemical technology and biotechnology 2019 v.94 no.1 pp. 136-146
ISSN:
0268-2575
Subject:
lysine; van der Waals forces; substrate specificity; Komagataella pastoris; serine proteinases; Carassius; arginine; amino acid substitution; hydrophobic bonding; computer simulation; enzymatic hydrolysis; mutants; hydrogen bonding; site-directed mutagenesis; circular dichroism spectroscopy
Abstract:
... BACKGROUND: Myofibril‐bound serine proteinase (MBSP) comprises a class of trypsin‐type serine proteinases that can specifically cleave the carboxyl side of peptide bonds of arginine or lysine residues. To obtain higher specificity MBSPs, mutants were designed based on the substrate‐binding pocket and constructed through site‐directed mutagenesis. RESULTS: The wild‐type (WT) and mutants were expres ...
DOI:
10.1002/jctb.5754

47. Alternative Assembly of α-Synuclein Leading to Protein Film Formation and Its Application for Developing Polydiacetylene-Based Sensing Materials

Author:
Soonkoo Lee; Daekyun Lee; Chul-Suk Hong; Jee Eun Yang; Jin Soo Kang; Yung-Eun Sung; Seung R. Paik
Source:
Langmuir 2019 v.35 no.36 pp. 11923-11931
ISSN:
1520-5827
Subject:
Fourier transform infrared spectroscopy; Parkinson disease; amyloid; biocompatible materials; circular dichroism spectroscopy; color; colorimetry; electrical conductivity; heat; oil-water interface; pH; solvents; temperature
Abstract:
... Understanding the self-assembly process of amyloidogenic protein is valuable not only to find its pathological implication but also to prepare protein-based biomaterials. α-Synuclein (αS), a pathological component of Parkinson’s disease, producing one-dimensional (1D) amyloid fibrils, has been employed to generate two-dimensional (2D) protein films by encouraging an alternative self-assembly proce ...
DOI:
10.1021/acs.langmuir.9b01593

48. Alternatone A, an Unusual Perylenequinone-Related Compound from a Soft-Coral-Derived Strain of the Fungus Alternaria alternata

Author:
Dong-Lin Zhao; Fei Cao; Chao-Yi Wang; Lu-Jia Yang; Ting Shi; Kai-Ling Wang; Chang-Lun Shao; Chang-Yun Wang
Source:
Journal of natural products 2019 v.82 no.11 pp. 3201-3204
ISSN:
1520-6025
Subject:
Alternaria alternata; X-ray diffraction; biochemical pathways; circular dichroism spectroscopy; cytotoxicity; fungi; human cell lines; inhibitory concentration 50; quinones; spectral analysis
Abstract:
... A novel perylenequinone-related compound, alternatone A (1), with an unprecedented tricyclo[6.3.1.0²,⁷] dodecane skeleton, together with three known perylenequinones, altertoxin I (2), stemphyperylenol (3), and alterperylenol (4), was isolated from the soft-coral-derived fungus Alternaria alternata L3111′. Their structures including absolute configurations were elucidated on the basis of comprehen ...
DOI:
10.1021/acs.jnatprod.9b00905

49. Amiaspochalasins A–H, Undescribed Aspochalasins with a C-21 Ester Carbonyl from Aspergillus micronesiensis

Author:
Zhaodi Wu; Xiaotian Zhang; Weaam Hasan Al Anbari; Mi Zhang; Xia Chen; Zengwei Luo; Xiao-Nian Li; Chunmei Chen; Junjun Liu; Jianping Wang; Hucheng Zhu; Yonghui Zhang
Source:
Journal of organic chemistry 2019 v.84 no.9 pp. 5483-5491
ISSN:
1520-6904
Subject:
Aspergillus; X-ray diffraction; antimicrobial properties; carbon; circular dichroism spectroscopy; cytotoxicity; diastereomers; esterification; hydrolysis; nuclear magnetic resonance spectroscopy; organic chemistry; organic compounds; spectral analysis
Abstract:
... Amiaspochalasins A–H (1–8), eight undescribed aspochalasins, and trichalasin D (9), a known analogue, were isolated from the solid culture of Aspergillus micronesiensis. Compounds 1–9 are aspochalasins with a C-21 ester carbonyl, and their structures were determined by spectroscopic data, X-ray crystallographic analyses, electronic circular dichroism calculations, and chemical methods. The CH₃-25 ...
DOI:
10.1021/acs.joc.9b00440

50. Amyloid fibers deriving from the aromatic core of C-terminal domain of nucleophosmin 1

Author:
Sara La Manna; Valentina Roviello; Pasqualina Liana Scognamiglio; Carlo Diaferia; Cinzia Giannini; Teresa Sibillano; Giancarlo Morelli; Ettore Novellino; Daniela Marasco
Source:
International journal of biological macromolecules 2019 v.122 pp. 517-525
ISSN:
0141-8130
Subject:
amino acid sequences; amyloid; calorimetry; circular dichroism spectroscopy; fluorescence emission spectroscopy; genes; ionization; mutation; myeloid leukemia; patients; scanning electron microscopy; titration; wide-angle X-ray scattering
Abstract:
... Nucleophosmin (NPM1) is the most frequently mutated gene in Acute Myeloid Leukemia (AML) patients and mutations lead to its aberrant cytoplasmatic accumulation in leukemic cells. Its C-terminal domain (CTD) is endowed with a globular structure consisting of a three-helix bundle in the wild type form that is disrupted by AML mutations. Our recent results demonstrate, unexpectedly and unequivocally, ...
DOI:
10.1016/j.ijbiomac.2018.10.210