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You searched for: Publication year rev 7981-2019 Remove constraint Publication year rev: 7981-2019 Subject term enzyme-linked immunosorbent assay Remove constraint Subject term: enzyme-linked immunosorbent assay
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1. (-)-Epigallocatechin-3-gallate suppresses cigarette smoke-induced inflammation in human cardiomyocytes via ROS-mediated MAPK and NF-κB pathways

Author:
Yingmin Liang; Mary Sau Man Ip; Judith Choi Wo Mak
Source:
Phytomedicine 2019 v.58 pp. 152768
ISSN:
0944-7113
Subject:
Camellia sinensis; Western blotting; acetylcysteine; apoptosis; cardiomyocytes; cardioprotective agents; cardiovascular diseases; catechin; chemokines; cigarettes; enzyme-linked immunosorbent assay; epigallocatechin gallate; flow cytometry; green tea; humans; inflammation; interleukins; leaves; mitochondria; mitogen-activated protein kinase; oxidative stress; reactive oxygen species; smoking (habit); transcription factor NF-kappa B
Abstract:
... Cigarette smoking is the leading cause for the initiation and development of cardiovascular disease (CVD). Oxidative stress and inflammatory responses play important roles in the pathophysiological processes of smoking-induced cardiac injury. (-)-epigallocatechin-3-gallate (EGCG), the most abundant catechin in green tea, which is made from Camellia sinensis leaves, has been reported to possess pot ...
DOI:
10.1016/j.phymed.2018.11.028

2. (Non-)Sense of Milk Testing in Small Ruminant Lentivirus Control Programs in Goats. Comparative Analysis of Antibody Detection and Molecular Diagnosis in Blood and Milk

Author:
Nadjah Radia Adjadj; Jo Vicca; Rodolphe Michiels; Nick De Regge
Source:
Viruses 2019 v.12 no.1 pp. -
ISSN:
1999-4915
Subject:
Lentivirus; antibody detection; blood sampling; blood serum; detection limit; diagnostic techniques; disease diagnosis; enzyme-linked immunosorbent assay; farms; goats; herds; milk; pellets; quantitative polymerase chain reaction; serology; Belgium
Abstract:
... Small ruminant lentivirus (SRLV) control programs are mainly based on diagnostic tests performed on blood samples collected from sheep and goats. Since blood sampling is costly and stressful for the animals, we evaluated whether milk could be used as an inexpensive and easily collectable matrix for SRLV detection. We therefore compared SRLV detection via two commercial enzyme-linked immunosorbent ...
DOI:
10.3390/v12010003

3. 106 Assessment of fecal near infrared reflectance spectroscopy to detect and monitor the reproductive status of endangered Amur and Snow leopard females

Author:
M. Santos-Rivera; L. Johnson-Ulrich; A. Graham; E. Willis; A. J. Kouba; C. K. Vance
Source:
Reproduction, fertility, and development 2019 v.31 no.1 pp. 179
ISSN:
1031-3613
Subject:
Ailuropoda melanoleuca; Panthera pardus; Panthera uncia; antelopes; carnivores; chemical industry; chemometrics; color; computer software; cost effectiveness; data collection; deer; enzyme-linked immunosorbent assay; feces; females; herbivores; least squares; methanol; models; near-infrared spectroscopy; normal values; principal component analysis; progesterone; variance; wavelengths; zoos
Abstract:
... Feces from captive and wild carnivores can yield valuable information about an individuals’ physiological and reproductive status, diet, and ecology. Near infrared spectroscopy (NIRS) is a rapid, noninvasive, cost-efficient technique widely used in the agricultural, pharmaceutical, and chemical industries that has gained traction in diagnostic and ecological field applications for herbivore specie ...
DOI:
10.1071/RDv31n1Ab106

4. 2D Graphene Oxide/Fe-MOF Nanozyme Nest with Superior Peroxidase-Like Activity and Its Application for Detection of Woodsmoke Exposure Biomarker

Author:
Xiaofan Ruan; Dong Liu; Xiangheng Niu; Yijia Wang; Christopher D. Simpson; Nan Cheng; Dan Du; Yuehe Lin
Source:
Analytical chemistry 2019 v.91 no.21 pp. 13847-13854
ISSN:
1520-6882
Subject:
active sites; benzo(a)pyrene; biomarkers; blood; catalytic activity; coordination polymers; enzyme-linked immunosorbent assay; enzymes; graphene; graphene oxide; humans; immunosensors; iron; metabolites; nanomaterials; nests; point-of-care systems; surface area; water solubility
Abstract:
... Emerging nanomaterials such as nanozymes have recently been applied for the immunoassay-based detection of biomarkers. However, the inferior catalytic activity and low water solubility of nanozymes remain as the major limitations compared to natural enzymes. To overcome these limitations, we successfully synthesized a superior nanozyme with a structure of enriched 2D catalytic interface, namely Na ...
DOI:
10.1021/acs.analchem.9b03321

5. 3D-Printed Pneumatic Microfluidic Mixer for Colorimetric Detection of Listeria monocytogenes

Author:
Ping Yao; Ronghui Wang; Xinge Xi; Yanbin Li; Steve Tung
Source:
Transactions of the ASABE v.62 no.3 pp. 841-850
ISSN:
2151-0032
Subject:
Listeria monocytogenes; air; color; colorimetry; enzyme-linked immunosorbent assay; prototypes; rapid methods; sample size; three-dimensional printing
Abstract:
... 3D printing can significantly improve the current fabrication techniques for microfluidic devices due to its ability to create truly 3D structures in a single step. In this study, an active pneumatic microfluidic mixer was designed and fabricated using an extrusion-based 3D printer and used for rapid detection of . The printed material of the mixer is flexible, semi-transparent, and inexpensive. T ...
DOI:
10.13031/trans.13245

6. 63 Bovine embryo-secreted microRNA-30c negatively regulates cell cycle progression through downregulation of CDK12

Author:
X. Lin; E. Beckers; S. Mc Cafferty; J. P. Catani; K. J. Szymanska; A. Van Soom; L. Peelman
Source:
Reproduction, fertility, and development 2019 v.31 no.1 pp. 157
ISSN:
1031-3613
Subject:
DNA; DNA damage; RNA libraries; Western blotting; apoptosis; biomarkers; blastocyst; cell communication; cell cycle; cell proliferation; cows; culture media; embryogenesis; enzyme-linked immunosorbent assay; flow cytometry; gene expression regulation; genes; kidney cells; luciferase; messenger RNA; microRNA; propidium; reverse transcriptase polymerase chain reaction; sequence analysis; staining; tumor suppressor proteins; water solubility
Abstract:
... Identifying microRNA (miRNA) differentially secreted by intermediate (cleaved between 26.6-31.4 hpi) and slow cleaving bovine embryos (cleaved after 31.4 hpi), investigating how they influence embryo development and consequently if they can be used as biomarkers for bovine embryo development. MicroRNA collected from conditioned media (CM) of 167 individually cultured embryos were sequenced on an I ...
DOI:
10.1071/RDv31n1Ab63

7. 7-Deoxy-trans-dihydronarciclasine Isolated from Lycoris chejuensis Inhibits Neuroinflammation in Experimental Models

Author:
Dong Zhao; Ming-Yao Gu; Li Jun Zhang; Hyo Jin Jeon; Yong-Baik Cho; Hyun Ok Yang
Source:
Journal of agricultural and food chemistry 2019 v.67 no.35 pp. 9796-9804
ISSN:
1520-5118
Subject:
Lycoris; Western blotting; active ingredients; cerebral cortex; enzyme-linked immunosorbent assay; inducible nitric oxide synthase; interleukin-10; interleukin-6; ionization; mice; neurodegenerative diseases; neuroglia; nitric oxide; pathophysiology; prostaglandin synthase; prostaglandins; secretion; transgenic animals; tumor necrosis factor-alpha
Abstract:
... Overactivated microglia and persistent neuroinflammation hold an important role in the pathophysiology of neurodegenerative diseases. The extract of Lycoris chejuensis (CJ) and its active compound, 7-deoxy-trans-dihydronarciclasine (named E144), attenuated expressions of pro-inflammatory factors, including nitric oxide, prostaglandin E₂, inducible nitric oxide synthase, cyclooxygenase-2 (COX-2), t ...
DOI:
10.1021/acs.jafc.9b03307

8. 74 Follicular fluid anti-Müllerian hormone concentration predicts juvenile ovine in vitro embryo development

Author:
J. E. Seccafien; J. M. Kelly; H. McGrice; D. O. Kleemann; K. L. Kind; W. H. E. J. van Wettere
Source:
Reproduction, fertility, and development 2019 v.31 no.1 pp. 162
ISSN:
1031-3613
Subject:
anti-Mullerian hormone; blastocyst; cattle; embryogenesis; enzyme-linked immunosorbent assay; ewes; follicular fluid; goats; gonadotropins; humans; juveniles; lambs; livestock and meat industry; oocytes; phenotype; pregnancy; regression analysis
Abstract:
... Currently, the commercial viability of assisted reproductive embryo technologies within the Australian livestock industry is restricted by individual variability in response to treatment protocols as well as oocyte developmental competence. The majority of losses come from embryo wastage, resulting from poor developmental competence during in vitro embryo production. Follicular fluid is readily av ...
DOI:
10.1071/RDv31n1Ab74

9. 90 Effects of treatment with the nonsteroidal anti-inflammatory drug Anafen prior to embryo recovery and the number of embryo flush procedures performed on prostaglandin levels in uterine fluid and pregnancy rate following embryo transfer

Author:
R. Dupras; L. Mills; C. Meunier; Y. Chorfi
Source:
Reproduction, fertility, and development 2019 v.31 no.1 pp. 171
ISSN:
1031-3613
Subject:
Holstein; catheters; cows; embryo transfer; enzyme-linked immunosorbent assay; ethylene glycol; heifers; ketoprofen; lactating females; lactation; pregnancy rate; prostaglandins; superovulation; t-test; uterus
Abstract:
... Some studies have shown that performing a second flush during embryo collection can increase the number of embryos recovered. However, this technique results in greater uterine manipulation that may increase PGF2α in the uterine fluid of donor cows and affect pregnancy rate in recipient females. Two experiments were conducted (1) to determine whether PGF2α concentration in uterine fluid was affect ...
DOI:
10.1071/RDv31n1Ab90

10. 91 Can propylene glycol modulate insulin and insulin-like growth factor-1 in superovulated dairy heifers?

Author:
R. Dupras; L. Mills; G. Robert; C. Meunier; Y. Chorfi
Source:
Reproduction, fertility, and development 2019 v.31 no.1 pp. 171
ISSN:
1031-3613
Subject:
Holstein; analysis of variance; artificial insemination; blood sampling; blood serum; catheters; chemiluminescence immunoassays; cloprostenol; controlled internal drug release devices; dairy heifers; embryogenesis; enzyme-linked immunosorbent assay; estrous cycle; gonadotropin-releasing hormone; insulin; insulin-like growth factor I; metabolism; oocytes; oral administration; progesterone; propylene glycol; secretion; superovulation; t-test; uterus; viability
Abstract:
... The aim of this study was to determine the effect of propylene glycol (PPG) on serum concentrations of insulin and insulin-like growth factor (IGF)-1 in superovulated dairy heifers. We hypothesised that administration of PPG would have a positive effect on superovulation results via increased insulin and IGF-1. A total of 20 clinically healthy Holstein heifers with an average age of 12 months were ...
DOI:
10.1071/RDv31n1Ab91

11. 95 N-Acetyl cysteine as a potential treatment for equine persistent breeding-induced endometritis

Author:
M. Caissie; T. Chenier; C. Gartley; E. Scholtz; R. Johnson; J. Hewson; D. Bienzle
Source:
Reproduction, fertility, and development 2019 v.31 no.1 pp. 173-174
ISSN:
1031-3613
Subject:
analysis of variance; anti-inflammatory activity; biopsy; breeding; clinical trials; cross-over studies; cysteine; edema; embryonic mortality; endometritis; endometrium; enzyme-linked immunosorbent assay; fibrosis; gene expression; genes; inflammation; insemination; interleukin-6; intrauterine administration; mares; neutrophils; nitric oxide; nitric oxide synthase; oviducts; protein content; quantitative polymerase chain reaction; risk factors; smooth muscle
Abstract:
... Persistent breeding-induced endometritis (PBIE) is a major cause of infertility in mares. Transient uterine inflammation is a normal response to breeding; however, PBIE-susceptible mares do not clear this inflammation in a timely fashion. Uterine inflammation at the time of embryonic descent from the oviducts ultimately results in early embryonic death and is seen clinically as infertility. Severa ...
DOI:
10.1071/RDv31n1Ab95

12. Elaeagnus glabra f. oxyphylla Attenuates Scopolamine-Induced Learning and Memory Impairments in Mice by Improving Cholinergic Transmission via Activation of CREB/NGF Signaling

Author:
Eunjin Sohn; Hye-Sun Lim; Yu Jin Kim; Bu-Yeo Kim; Joo-Hwan Kim; Soo-Jin Jeong
Source:
Nutrients 2019 v.11 no.6 pp. -
ISSN:
2072-6643
Subject:
Elaeagnus; Western blotting; acetylcholine; acetylcholinesterase; animal models; apoptosis; brain; caspase-3; choline acetyltransferase; cognition; cyclic AMP; enzyme-linked immunosorbent assay; males; memory; memory disorders; mice; nerve growth factor; protein synthesis; proteins; scopolamine; staining; therapeutics; tissues
Abstract:
... We aimed to investigate the therapeutic effects of an Elaeagnus glabra f. oxyphylla (EGFO) ethanol extract in mice with scopolamine-induced memory dysfunction. Fifty male mice were randomly divided into a normal control group, a scopolamine-treated group, a scopolamine and EGFO extract-treated group, and a scopolamine and tacrine-treated group. EGFO (50 or 100 mg/kg/day) was received for 21 days. ...
DOI:
10.3390/nu11061205
PubMed:
31141948
PubMed Central:
PMC6627942

13. Mycobacterium avium subsp. paratuberculosis ELISA Responses in Milk Samples from Vaccinated and Nonvaccinated Dairy Goat Herds in The Netherlands

Author:
Saskia Luttikholt; Karianne Lievaart-Peterson; Maaike Gonggrijp; Marian Aalberts; Gerdien van Schaik; Piet Vellema
Source:
Veterinary sciences 2019 v.6 no.2 pp. -
ISSN:
2306-7381
Subject:
Mycobacterium avium subsp. paratuberculosis; blood serum; dairy goats; enzyme-linked immunosorbent assay; herd size; milk; paratuberculosis; Netherlands
Abstract:
... The aims of our study were to calculate the most appropriate cut-off value for milk samples in a serum-validated Mycobacterium avium subsp. paratuberculosis (MAP) ELISA and to analyze MAP ELISA responses in milk samples from vaccinated and nonvaccinated dairy goats in the Netherlands. Analyzed herds were representative for location and herd size of dairy goat herds in the Netherlands. A significan ...
DOI:
10.3390/vetsci6020058
PubMed:
31234509
PubMed Central:
PMC6632119

14. Salix tetrasperma Roxb. Extract Alleviates Neuropathic Pain in Rats via Modulation of the NF-κB/TNF-α/NOX/iNOS Pathway

Author:
Mansour Sobeh; Mona F. Mahmoud; Samar Rezq; Amira E. Alsemeh; Omar M. Sabry; Islam Mostafa; Mohamed A. O. Abdelfattah; Khadija Ait El-Allem; Assem M. El-Shazly; Aziz Yasri; Michael Wink
Source:
Antioxidants 2019 v.8 no.10 pp. -
ISSN:
2076-3921
Subject:
NAD(P)H oxidase (H2O2-forming); Salix; Schwann cells; animal models; antioxidant activity; biomarkers; brain stem; burning; dose response; enzyme inhibition; enzyme-linked immunosorbent assay; flowers; high performance liquid chromatography; inducible nitric oxide synthase; inflammation; methanol; myelin sheath; nerve tissue; oxidative stress; pain; patients; prostaglandin synthase; prostaglandins; rats; secondary metabolites; somatosensory disorders; tandem mass spectrometry; traditional medicine; transcription factor NF-kappa B; tumor necrosis factor-alpha
Abstract:
... Patients with neuropathic pain experience chronic painful tingling, burning, and prickling sensations accompanied with hyperalgesia and/or allodynia. In this study, 38 secondary metabolites of a methanol extract from Salix tetrasperma flowers were identified by liquid chromatography-mass spectrometry (HPLC-MS/MS). The extract showed substantial anti-inflammatory, central and peripheral anti-nocice ...
DOI:
10.3390/antiox8100482
PubMed:
31614846
PubMed Central:
PMC6826723

15. A European inter-laboratory trial to evaluate the performance of three serological methods for diagnosis of Mycoplasma bovis infection in cattle using latent class analysis

Author:
Anna-Maria Andersson; Anna Aspán; Henk J. Wisselink; Bregtje Smid; Anne Ridley; Sinikka Pelkonen; Tiina Autio; Klara Tølbøll Lauritsen; Jane Kensø; Patrice Gaurivaud; Florence Tardy
Source:
BMC veterinary research 2019 v.15 no.1 pp. 369
ISSN:
1746-6148
Subject:
Mycoplasma bovis; Western blotting; antibodies; cattle; cattle diseases; disease prevalence; enzyme-linked immunosorbent assay; herds; laboratory diagnosis; pathogens; researchers; Europe
Abstract:
... BACKGROUND: Mycoplasma bovis (M. bovis) is an emerging bovine pathogen, leading to significant economic losses in the livestock industry worldwide. Infection can result in a variety of clinical signs, such as arthritis, pneumonia, mastitis and keratoconjunctivitis, none of which are M. bovis-specific. Laboratory diagnosis is therefore important. Serological tests to detect M. bovis antibodies is c ...
DOI:
10.1186/s12917-019-2117-0
PubMed:
31653217
PubMed Central:
PMC6814985

16. A Large Cohort of Neurocysticercosis in Shandong Province, Eastern China, 1997–2015

Author:
Xiaodan Huang; Zhonglei Wang; Jingxuan Kou; Hongmei Liu; Dehua Mao; Zhenhua Yu; Xin Liu; Peng Cheng; Maoqing Gong
Source:
Vector borne and zoonotic diseases 2019 v.19 no.12 pp. 901-907
ISSN:
1557-7759
Subject:
albendazole; antibodies; antigens; blood serum; cognition; computed tomography; enzyme-linked immunosorbent assay; headache; hemagglutination; memory disorders; meningoencephalitis; neurocysticercosis; patients; praziquantel; seizures; therapeutics; China
Abstract:
... Neurocysticercosis (NCC) has a wide spectrum of neurologic and psychiatric manifestations, including epileptic seizures, high intracranial pressure, cognitive dysfunction, and meningoencephalitis. This study presents the clinical diagnosis of 2539 NCC patients in Shandong Province, Eastern China, from 1997 to 2015. The diagnosis was based primarily on clinical features, neuroimaging, immunology, a ...
DOI:
10.1089/vbz.2019.2447
PubMed:
31314709
PubMed Central:
PMC6882449

17. A Large-Scale Serological Survey of Toxoplasma gondii Infection Among Persons Participated in Health Screening in Yunnan Province, Southwestern China

Author:
Zhao Li; Chun-Yi Sun; Ren-Dan Yang; Chang-Jun Xu; Mei-Chen Chen; Yun-Jiang Zhang; Wei Cong; Xing-Quan Zhu; Yang Liu; Feng-Cai Zou
Source:
Vector borne and zoonotic diseases 2019 v.19 no.6 pp. 441-445
ISSN:
1557-7759
Subject:
Toxoplasma gondii; antibodies; blood serum; enzyme-linked immunosorbent assay; females; hospitals; humans; immunoglobulin G; immunoglobulin M; males; people; public health; screening; serological surveys; seroprevalence; toxoplasmosis; China
Abstract:
... Surveys of Toxoplasma gondii infection in animals have been reported in Yunnan province, southwestern China. However, limited information is available regarding the epidemiology of T. gondii infection among persons participated in health screening in Yunnan. From January 2014 to December 2016, a large-scale and cross-sectional serological survey was conducted to reveal the seroprevalence of T. gon ...
DOI:
10.1089/vbz.2018.2334

18. A Multi-Hemagglutinin-Based Enzyme-Linked Immunosorbent Assay to Serologically Detect Influenza A Virus Infection in Animals

Author:
Miki Okumura; Akiko Takenaka-Uema; Shin Murakami; Taisuke Horimoto
Source:
Veterinary sciences 2019 v.6 no.3 pp. -
ISSN:
2306-7381
Subject:
Influenza A virus; antibodies; antigens; antiserum; avian influenza; enzyme-linked immunosorbent assay; hemagglutinins; intermediate hosts; mammals; mutants; pandemic; risk assessment
Abstract:
... Mammals can play a role as an intermediate host in the emergence of mammalian-adapted reassortants or mutants of avian influenza A viruses, with pandemic potential. Therefore, detecting viral infection in animals followed by assessment of the hemagglutinin (HA) subtype of the agent is an indispensable process for risk assessment in pandemic preparedness. In this study, we tested the potential of a ...
DOI:
10.3390/vetsci6030064
PubMed:
31336638
PubMed Central:
PMC6789898

19. A Multicolor Immunosensor for Sensitive Visual Detection of Breast Cancer Biomarker Based on Sensitive NADH-Ascorbic-Acid-Mediated Growth of Gold Nanobipyramids

Author:
Zongwen Wang; Qian Chen; Yingying Zhong; Xinhui Yu; Yongning Wu; FengFu Fu
Source:
Analytical chemistry 2019 v.92 no.1 pp. 1534-1540
ISSN:
1520-6882
Subject:
NAD (coenzyme); antibodies; ascorbic acid; biomarkers; blood serum; breast neoplasms; color; detection limit; early diagnosis; enzyme-linked immunosorbent assay; erbB-2 receptor; gold; humans; immunosensors; monitoring; nanogold; therapeutics; ultraviolet-visible spectroscopy
Abstract:
... Many studies have demonstrated that the extracellular domain of human epidermal growth factor receptor 2 (HER2 ECD) level in serum can act as a breast cancer biomarker and serve as a monitoring neoadjuvant therapy of breast cancer. In this study, we developed a sensitive ascorbic acid (AA)-mediated AuNBPs (gold nanobipyramids) growth method with NADH (reduced nicotinamide adenine dinucleotide I) a ...
DOI:
10.1021/acs.analchem.9b04828

20. A New Tymovirus Isolated From Solanum quitoense: Characterization and Prevalence in Two Solanaceous Crops in Ecuador

Author:
Juan F. Cornejo-Franco; Robert A. Alvarez-Quinto; Samuel Grinstead; Dimitre Mollov; Alexander V. Karasev; Jose Ochoa; Diego F. Quito-Avila
Source:
Plant disease 2019 v.103 no.9 pp. 2246-2251
ISSN:
0191-2917
Subject:
Chiltepin yellow mosaic virus; Eggplant mosaic virus; Solanum betaceum; Solanum quitoense; antibodies; certification; cultivars; enzyme-linked immunosorbent assay; fresh market; genome; hybrids; juices; nucleotides; open reading frames; orchards; phylogeny; potatoes; reverse transcriptase polymerase chain reaction; surveys; viruses; Ecuador
Abstract:
... Naranjilla (Solanum quitoense Lam.) and tamarillo (S. betaceum Cav.) are two important perennial solanaceous crops grown in Ecuador for the fresh market and juice production. Viruses infecting tamarillo and naranjilla are currently poorly studied, and no clean stock program exists in Ecuador. Here, we report a new virus, provisionally named as naranjilla mild mosaic virus (NarMMV) (genus Tymovirus ...
DOI:
10.1094/PDIS-01-19-0113-RE

21. A Novel Indirect Competitive Enzyme-Linked Immunosorbent Assay Format for the Simultaneous Determination of Ractopamine and Phenylethanolamine A Residues in Swine Urine

Author:
Dapeng Peng; Lan Zhao; Lingyan Zhang; Yuanhu Pan; Yanfei Tao; Yulian Wang; Feng Sheng; Zonghui Yuan
Source:
Food analytical methods 2019 v.12 no.5 pp. 1077-1085
ISSN:
1936-9751
Subject:
antigens; coatings; detection limit; enzyme-linked immunosorbent assay; high performance liquid chromatography; mass spectrometry; monitoring; monoclonal antibodies; ractopamine; swine; urine
Abstract:
... For monitoring ractopamine (RAC) and phenylethanolamine A (PA) with high efficiency, a novel indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) format was developed. At first, two specific monoclonal antibodies (mAbs) against RAC and PA were prepared, respectively. Based on the finding that both mAbs RAC and PA could recognize the same coating antigen RAC-SA-OVA, a novel ic-ELISA fo ...
DOI:
10.1007/s12161-019-01445-3

22. A One Health systematic review of diagnostic tools for Echinococcus multilocularis surveillance: Towards equity in global detection

Author:
Janna M. Schurer; Arlene Nishimwe; Dieudonne Hakizimana; Huan Li; Yu Huang; Jean Pierre Musabyimana; Eugene Tuyishime; Lauren E. MacDonald
Source:
Food and Waterborne Parasitology 2019 v.15 pp. e00048
ISSN:
2405-6766
Subject:
Canidae; Echinococcus multilocularis; One Health initiative; Western blotting; coproantigens; data collection; databases; definitive hosts; diagnostic sensitivity; diagnostic techniques; echinococcosis; environmental monitoring; enzyme-linked immunosorbent assay; humans; image analysis; immunoaffinity chromatography; intestines; magnetism; parasites; patients; people; politics; reverse transcriptase polymerase chain reaction; soil; systematic review; Asia; Europe; North America
Abstract:
... Echinococcus multilocularis is a zoonotic cestode of canid definitive hosts that is emerging as a parasite of medical and veterinary concern in regions of North America, Europe and Asia. Infection with the metacestode stage (alveolar echinococcosis – AE) is life-threatening, especially for patients who reside in low resource countries and lack access to modern diagnostic tests and treatments. The ...
DOI:
10.1016/j.fawpar.2019.e00048
PubMed:
32095619
PubMed Central:
PMC7034026

23. A Practical ELISA for Azaspiracids in Shellfish via Development of a New Plate-Coating Antigen

Author:
Ingunn A. Samdal; Kjersti E. Løvberg; Anja B. Kristoffersen; Lyn R. Briggs; Jane Kilcoyne; Craig J. Forsyth; Christopher O. Miles
Source:
Journal of agricultural and food chemistry 2019 v.67 no.8 pp. 2369-2376
ISSN:
1520-5118
Subject:
antigens; azaspiracid; cross reaction; enzyme-linked immunosorbent assay; liquid chromatography; mass spectrometry; screening; shellfish; toxins
Abstract:
... Azaspiracids (AZAs) are a group of biotoxins that appear periodically in shellfish and can cause food poisoning in humans. Current methods for quantifying the regulated AZAs are restricted to LC-MS but are not well suited to detecting novel and unregulated AZAs. An ELISA method for total AZAs in shellfish was reported recently, but unfortunately, it used relatively large amounts of the AZA-1-conta ...
DOI:
10.1021/acs.jafc.8b05652

24. A Prussian Blue ZnO Carbon Nanotube Composite for Chronoamperometrically Assaying H₂O₂ in BT20 and 4T1 Breast Cancer Cells

Author:
Raja Ram Pandey; Yuhang Guo; Ying Gao; Charles C. Chusuei
Source:
Analytical chemistry 2019 v.91 no.16 pp. 10573-10581
ISSN:
1520-6882
Subject:
apoptosis; breast neoplasms; carbon nanotubes; catalysts; cell lines; electrochemistry; electrostatic interactions; enzyme-linked immunosorbent assay; glassy carbon electrode; hydrogen peroxide; mixing; monitoring; nanoparticles; neoplasm cells; pH; zinc oxide
Abstract:
... A Prussian Blue (PB) zinc oxide carbon nanotube sensing composite was developed for the rapid assaying of H₂O₂ generated from BT20 and 4T1 breast cancer cells, important for elucidating mechanisms governing apoptosis of these cell lines. The combination of H₂O₂’s transient nature along with matrix effects makes monitoring this molecule in biological samples a challenge. The standard addition metho ...
DOI:
10.1021/acs.analchem.9b01636

25. A Real-Time PCR Differentiating Pantoea stewartii subsp. stewartii From P. stewartii subsp. indologenes in Corn Seed

Author:
Narinder Pal; Charles C. Block; Candice A. C. Gardner
Source:
Plant disease 2019 v.103 no.7 pp. 1474-1486
ISSN:
0191-2917
Subject:
Pantoea stewartii subsp. indologenes; Pantoea stewartii subsp. stewartii; Zea mays; bacteria; corn; enzyme-linked immunosorbent assay; false positive results; intergenic DNA; quantitative polymerase chain reaction; seed-borne diseases; seeds
Abstract:
... Stewart’s wilt of corn caused by the bacterium Pantoea stewartii subsp. stewartii is a seed-borne disease of major phytosanitary importance. Many countries have imposed restrictions on corn seed imports from regions where the disease occurs to prevent the potential introduction of the pathogen. Current laboratory testing methods (enzyme-linked immunosorbent assay [ELISA] and polymerase chain react ...
DOI:
10.1094/PDIS-06-18-0936-RE

26. A Recombinant Turkey Herpesvirus Expressing F and HN Genes of Avian Avulavirus-1 (AAvV-1) Genotype VI Confers Cross-Protection against Challenge with Virulent AAvV-1 Genotypes IV and VII in Chickens

Author:
Krzysztof Śmietanka; Jolanta Tyborowska; Monika Olszewska-Tomczyk; Katarzyna Domańska-Blicharz; Zenon Minta; Lukasz Rabalski; Anna Czarnota; Krzysztof Kucharczyk; Boguslaw Szewczyk
Source:
Viruses 2019 v.11 no.9 pp. -
ISSN:
1999-4915
Subject:
Meleagrid alphaherpesvirus 1; Newcastle disease; Paramyxoviridae; animal viruses; antibodies; broiler chickens; cross immunity; enzyme-linked immunosorbent assay; financial economics; genes; genotype; hemagglutination inhibition test; humoral immunity; immunization; poultry industry; specific pathogen-free animals; turkeys; viral vaccines; virulence; virulent strains
Abstract:
... Newcastle disease (ND) is responsible for significant economic losses in the poultry industry. The disease is caused by virulent strains of Avian avulavirus 1 (AAvV-1), a species within the family Paramyxoviridae. We developed a recombinant construct based on the herpesvirus of turkeys (HVT) as a vector expressing two genes: F and HN (HVT-NDV-F-HN) derived from the AAvV-1 genotype VI (“pigeo ...
DOI:
10.3390/v11090784
PubMed:
31450681
PubMed Central:
PMC6784189

27. A SERS microfluidic platform for targeting multiple soluble immune checkpoints

Author:
K.Kamil Reza; Abu Ali Ibn Sina; Alain Wuethrich; Yadveer S. Grewal; Christopher B. Howard; Darren Korbie; Matt Trau
Source:
Biosensors & bioelectronics 2019 v.126 pp. 178-186
ISSN:
0956-5663
Subject:
Raman spectroscopy; biomarkers; biopsy; biosensors; blood serum; chemical species; enzyme-linked immunosorbent assay; graphene oxide; humans; immunotherapy; manufacturing; monitoring; monoclonal antibodies; neoplasms; proteins; yeasts
Abstract:
... Immune checkpoint blockade therapies are promising next generation immunotherapeutic treatments for cancer. Whilst sequential solid biopsies are an invaluable source of prognostic information, they are not feasible for monitoring therapeutic outcomes over time. Monitoring soluble immune checkpoint markers expression in body fluids could potentially be a better alternative. Current methods (e.g. EL ...
DOI:
10.1016/j.bios.2018.10.044

28. A Serological Survey of Borrelia burgdorferi Infection in Sheep in Northeast China Regions Through Outer Surface Protein C-Based Enzyme-Linked Immunosorbent Assay

Author:
Xunliang Li; Pengchong Li; Tongyuan Zhang; Pengkun Zhang; Xiaofeng Ren; Guangxing Li
Source:
Vector borne and zoonotic diseases 2019 v.19 no.1 pp. 16-21
ISSN:
1557-7759
Subject:
Borrelia burgdorferi; Ixodes; Lyme disease; antibodies; blood serum; disease transmission; enzyme-linked immunosorbent assay; etiological agents; secretion; serological surveys; sheep; sheep diseases; specific pathogen-free animals; surface proteins; ticks; China
Abstract:
... Borrelia burgdorferi as a causative agent of Lyme disease is transmitted by Ixodes spp. ticks to humans and animals. Sheep is considered a natural reservoir for B. burgdorferi and plays a pivotal role in disease transmission and the expansion of natural foci. An epidemiological investigation of B. burgdorferi in sheep is essential for prevention and control of Lyme disease. In this study, we devel ...
DOI:
10.1089/vbz.2018.2268

29. A Short Study Exploring the Effect of the Glycaemic Index of the Diet on Energy intake and Salivary Steroid Hormones

Author:
Emad A.S. Al-Dujaili; Sophie Ashmore; Catherine Tsang
Source:
Nutrients 2019 v.11 no.2 pp. -
ISSN:
2072-6643
Subject:
blood glucose; cortisol; diet; energy intake; enzyme-linked immunosorbent assay; females; foods; glucose; glycemic index; saliva; steroid hormones; testosterone; volunteers
Abstract:
... Background: The glycaemic index or load (GI or GL) is a concept for ranking carbohydrate-rich foods based on the postprandial blood glucose response compared with a reference food (glucose). Due to the limited research investigating the effect of the GI or GL of the diet on salivary steroidal hormones, this explorative short study was conducted. Methods: 12 female participants consumed a low GI an ...
DOI:
10.3390/nu11020260
PubMed:
30682835
PubMed Central:
PMC6413178

30. A broad spectrum monoclonal antibody against porcine circovirus type 2 for antigen and antibody detection

Author:
Liping Huang; Yanwu Wei; Deli Xia; Dan Liu; Hongzhen Zhu; Hongli Wu; Li Feng; Changming Liu
Source:
Applied microbiology and biotechnology 2019 v.103 no.8 pp. 3453-3464
ISSN:
0175-7598
Subject:
Porcine circovirus-2; Western blotting; absorbance; antibody detection; antigens; enzyme-linked immunosorbent assay; enzymes; hybridomas; immunization; immunoperoxidase monolayer assay; lymph nodes; monitoring; monoclonal antibodies; neutralization; neutralization tests; piglets; seroconversion; viral load
Abstract:
... This study described the production, characterization, and application of monoclonal antibodies (mAbs) against porcine circovirus type 2 (PCV2). Twelve stable hybridomas were produced by immunization with purified PCV2a/LG strain and characterized by immunoperoxidase monolayer assay (IPMA), Western blotting, and neutralization assays. All mAbs could react with the PCV2 Cap protein and neutralize P ...
DOI:
10.1007/s00253-019-09715-0

31. A cell ELISA based method for exosome detection in diagnostic and therapeutic applications

Author:
Shabanali Khodashenas; Saeed Khalili; Mehdi Forouzandeh Moghadam
Source:
Biotechnology letters 2019 v.41 no.4-5 pp. 523-531
ISSN:
0141-5492
Subject:
Western blotting; antigens; blood serum; cell viability; disease diagnosis; enzyme-linked immunosorbent assay; exosomes; genes; in vitro studies; messenger RNA; microRNA; neoplasms; paper; proteins; saliva; semen; therapeutics; transmission electron microscopy
Abstract:
... OBJECTIVE: Exosomes are nano-scaled carriers of miRNA, mRNA and proteins which are secreted from viable cells. Exosome detection within serum, saliva and semen offers diagnostic value for detection of various diseases including cancer. In the present study, we have lunched an in vitro study to develop a more efficient method for exosome detection. In this regard, the recombinant LAMP-DARPins (capa ...
DOI:
10.1007/s10529-019-02667-5

32. A colorimetric immunosensor based on self-linkable dual-nanozyme for ultrasensitive bladder cancer diagnosis and prognosis monitoring

Author:
Chen Peng; Mu-Yi Hua; Nan-Si Li; Ying-Pei Hsu; Ying-Tzu Chen; Cheng-Keng Chuang; See-Tong Pang; Hung-Wei Yang
Source:
Biosensors & bioelectronics 2019 v.126 pp. 581-589
ISSN:
0956-5663
Subject:
absorption; antibodies; apolipoprotein A-I; colorimetry; enzyme-linked immunosorbent assay; goats; graphene oxide; hydrogen peroxide; immunoglobulin G; immunosensors; magnetism; mice; monitoring; patients; prognosis; rabbits; urinary bladder neoplasms; urine
Abstract:
... We developed self-linkable Prussian blue (PB)-incorporated magnetic graphene oxide (PMGO) as a peroxidase-mimicking nanozyme with high oxidizability to 3,3′,5,5′-tetramethylbenzidine (TMB), which generates significant absorption intensity for the colorimetric immunosensing of apolipoprotein A1 (ApoA1) in early bladder cancer (BC) diagnosis and prognosis monitoring. The ultrasensitive immunosensor ...
DOI:
10.1016/j.bios.2018.11.022

33. A commercial ELISA for detection of interferon gamma in white rhinoceros

Author:
Josephine Chileshe; Wynand J. Goosen; Peter E. Buss; Paul D. van Helden; Robin Warren; Sven D. C. Parsons; Michele A. Miller
Source:
Journal of veterinary diagnostic investigation 2019 v.31 no.4 pp. 531-536
ISSN:
1943-4936
Subject:
Ceratotherium simum; Mycobacterium bovis; antibodies; blood; bovine tuberculosis; enzyme-linked immunosorbent assay; horses; interferon-gamma; temperature; South Africa
Abstract:
... Bovine tuberculosis (bTB), caused by Mycobacterium bovis, is endemic in Kruger National Park, South Africa, home to the largest population of white rhinoceros (Ceratotherium simum) in the world. In 2016, the first cases of naturally occurring bTB were reported in white rhinoceros; however, there is a lack of understanding of infection and disease process in this species. Prevention and control of ...
DOI:
10.1177/1040638719843955
PubMed:
30973098
PubMed Central:
PMC6857013

34. A comparison of Taenia solium and Taenia hydatigena infection in pigs using serological diagnosis and post-mortem inspection methods in Benoué division, North Cameroon

Author:
Emmanuel Assana; Julius Awah-Ndukum; Justin D. Djonmaïla; Hidrice D. Djiatche; Charles Awé; T.K. Manchang; André P. Zoli
Source:
Veterinary Parasitology: Regional Studies and Reports 2019 v.17 pp. 100306
ISSN:
2405-9390
Subject:
Bayesian theory; Taenia hydatigena; Taenia multiceps; Taenia saginata; Taenia solium; antibodies; blood serum; cysticerci; enzyme-linked immunosorbent assay; epilepsy; heart; muscles; necropsy; neurocysticercosis; parasites; serodiagnosis; swine; swine diseases; Cameroon
Abstract:
... The metacestodes of Taenia solium and Taenia hydatigena are the cause of cysticercosis in pigs. T. solium is also responsible of the taeniosis/neurocysticercosis complex in humans, constituting a main cause of epilepsy cases across endemic countries. T. hydatigena is non-zoonotic, but its occurrence in pigs contributes significantly to false positive reactions should genus-species serological meth ...
DOI:
10.1016/j.vprsr.2019.100306

35. A comparison of different vaccination schemes used in sheep combining inactivated bluetongue vaccines against serotypes 4 and 8

Author:
Johanna Hilke; Heinz Strobel; Soeren Woelke; Melanie Stoeter; Katja Voigt; Lucie Grimm; Johanna Meilwes; Teresa Punsmann; Irena Blaha; Andreas Salditt; Karl Rohn; Max Bastian; Martin Ganter
Source:
Vaccine 2019 v.37 no.39 pp. 5844-5853
ISSN:
0264-410X
Subject:
blood serum; bluetongue; enzyme-linked immunosorbent assay; immune response; immunogenicity; injection site; neutralization tests; neutralizing antibodies; seroprevalence; serotypes; sheep; vaccination; vaccines
Abstract:
... Eight different vaccination schemes using four commercially available inactivated Bluetongue vaccines against serotypes 4 and 8 in three different combinations (setting 1–3) were tested under field conditions for their ability to generate a measurable immune response in sheep.Animals of setting 1 (groups A–D) were simultaneously vaccinated using either individual injections at different locations ...
DOI:
10.1016/j.vaccine.2019.08.011

36. A competitive enzyme-linked immunosorbent assay for rapid detection of antibodies against Trichinella spiralis and T. britovi – one test for humans and swine

Author:
M. Gnjatovic; A. Gruden-Movsesijan; N. Miladinovic-Tasic; N. Ilic; S. Vasilev; J. Cvetkovic; Lj. Sofronic-Milosavljevic
Source:
Journal of helminthology 2019 v.93 no.1 pp. 33-41
ISSN:
1475-2697
Subject:
Trichinella britovi; Trichinella spiralis; Western blotting; enzyme-linked immunosorbent assay; epitopes; horses; hosts; humans; meat; monitoring; monoclonal antibodies; muscle larvae; parasites; rapid methods; swine; trichinosis; wild animals
Abstract:
... Infection with parasites from the Trichinella genus occurs in many vertebrates but disease only occurs in humans (trichinellosis). Humans are infected after the consumption of raw or undercooked meat from infected wild or domestic animals (usually swine or horses). Using the monoclonal antibody (mAb) 7C2C5, specific for an epitope unique to the muscle larvae of the genus Trichinella, we have devel ...
DOI:
10.1017/S0022149X17001092
PubMed:
29168448

37. A critical review of the specifications and performance of antibody and DNA-based methods for detection and quantification of allergens in foods

Author:
Hamide Z. Senyuva; Ivona Baricevic Jones; Mark Sykes; Sabine Baumgartner
Source:
Food additives & contaminants 2019 v.36 no.4 pp. 507-547
ISSN:
1944-0057
Subject:
DNA; allergens; analytical kits; antibodies; commercialization; eggs; enzyme-linked immunosorbent assay; food industry; foods; mass spectrometry; milk; peanuts; polymerase chain reaction
Abstract:
... Despite the availability of a large number of antibody and DNA based methods for detection and quantification of allergens in food there remain significant difficulties in selecting the optimum technique to employ. Published methods from research groups mostly contain sufficient detail concerning target antigen, calibration procedures and method performance to allow replication by others. However, ...
DOI:
10.1080/19440049.2019.1579927

38. A defective release of host defense peptides is present in canine atopic skin

Author:
Domenico Santoro; Linda Archer; Karen Kelley
Source:
Comparative immunology, microbiology, and infectious diseases 2019 v.65 pp. 65-69
ISSN:
0147-9571
Subject:
adhesion; animal models; atopic dermatitis; dogs; enzyme-linked immunosorbent assay; fluorescent antibody technique; humans; microscopy; pathogenesis; peptides; secretion; skin (animal)
Abstract:
... The use of dogs as animal model for human atopic dermatitis (AD) is well known. Striking similarities in the pathogenesis of AD have been demonstrated. Similar alteration of host defense peptides (HDP) have been identified in both species. However, the ultrastructural/molecular alterations associated with HDPs secretion in AD have not been elucidated. We were able to use a multidisciplinary approa ...
DOI:
10.1016/j.cimid.2019.04.008

39. A direct comparison of liquid chromatography-mass spectrometry with clinical routine testing immunoassay methods for the detection and quantification of thyroid hormones in blood serum

Author:
Samantha L. Bowerbank; Michelle G. Carlin; John R. Dean
Source:
Analytical and bioanalytical chemistry 2019 v.411 no.13 pp. 2839-2853
ISSN:
1618-2642
Subject:
blood serum; capital; electrochemiluminescence; enzyme-linked immunosorbent assay; formic acid; humans; liquid chromatography; mass spectrometry; methanol; pH; solid phase extraction; thyroid hormones
Abstract:
... A new and improved method was developed for the determination and quantification of four “free” thyroid hormones (i.e. 3,5-diiodothyronine (T2), 3,3′,5-triiodothyronine (T3), 3,3′,5′-triiodothyrone (rT3) and 3,5,3′,5′-tetraiodothyronine (T4)) in human serum by low- and high-resolution liquid chromatography-mass spectrometry (LC-MS). Several sample preparation strategies were investigated to obtain ...
DOI:
10.1007/s00216-019-01724-2
PubMed:
31079177
PubMed Central:
PMC6522465

40. A divergent strain of melon chlorotic spot virus isolated from black medic (Medicago lupulina) in Austria

Author:
Yahya Z. A. Gaafar; Katja R. Richert-Pöggeler; Angelika Sieg-Müller; Petra Lüddecke; Kerstin Herz; Jonas Hartrick; Yvonne Seide; Heinrich-Josef Vetten; Heiko Ziebell
Source:
Virology journal 2019 v.16 no.1 pp. 89
ISSN:
1743-422X
Subject:
Medicago lupulina; Medicago sativa; Nicotiana benthamiana; Pisum sativum; RNA; Tenuivirus; Vicia faba; diagnostic techniques; enzyme-linked immunosorbent assay; genome; high-throughput nucleotide sequencing; melons; polyclonal antibodies; viral proteins; viruses; Austria; France
Abstract:
... A tenuivirus, referred to here as JKI 29327, was isolated from a black medic (Medicago lupulina) plant collected in Austria. The virus was mechanically transmitted to Nicotiana benthamiana, M. lupulina, M. sativa, Pisum sativum and Vicia faba. The complete genome was determined by high throughput sequencing. The genome of JKI 29327 consists of eight RNA segments closely related to those of melon c ...
DOI:
10.1186/s12985-019-1195-8
PubMed:
31277670
PubMed Central:
PMC6612211

41. A fluorescence immunoassay based on CdTe : Zn/ZnS quantum dots for the rapid detection of bacteria, taking Delftia tsuruhatensis CM’13 as an example

Author:
Yao Ruan; Huanhuan Xu; Jinlu Yu; Qian Chen; Lihong Gu; Ailing Guo
Source:
RSC advances 2019 v.10 no.2 pp. 1042-1049
ISSN:
2046-2069
Subject:
Delftia; antibodies; antigens; biocompatibility; detection limit; enzyme-linked immunosorbent assay; fluorescence; fluorescent antibody technique; goats; photoluminescence; rapid methods
Abstract:
... A fluorescence immunoassay has been widely applied in different fields due to its high sensitivity, simple operations, and high accuracy. Quantum dots (QDs) are often selected as labels in a fluorescence immunoassay due to their high fluorescence, better stability, and biocompatibility. In this study, novel doped CdTe : Zn/ZnS QDs with stability and a high photoluminescence quantum yield (40.78%) ...
DOI:
10.1039/c9ra08651j

42. A fluorescent molecularly imprinted device for the on-line analysis of AFP in human serum

Author:
Run-tian Ma; Xiao-bo Zhao; Xiao-yu Sun; Jia Liu; Wei Ha; Yan-ping Shi
Source:
Journal of materials chemistry B 2019 v.7 no.40 pp. 6187-6194
ISSN:
2050-7518
Subject:
antibodies; blood serum; boronic acids; enzyme-linked immunosorbent assay; fluorescence; humans; hydrolysis; molecular imprinting; nanomaterials; neoplasms; screening; signal transduction
Abstract:
... MIT is a promising strategy in antibody free analysis for tumour markers. Conventional nanosized MIPs with off-line analysis are beset by tedious operation and unsatisfactory analysis performance. In this work, an on-line analytical device to directly detect AFP, which is a typical tumour marker in cancer screening, was prepared for the first time. A microscope slide was chosen to be the basis of ...
DOI:
10.1039/c9tb01331h

43. A generic method for the detection of polyethylene glycol specific IgG and IgM antibodies in human serum

Author:
Christopher Ehlinger; Nathan Spear; Rajitha Doddareddy; Gopi Shankar; Allen Schantz
Source:
Journal of immunological methods 2019 pp. -
ISSN:
0022-1759
Subject:
Western blotting; additives; antibodies; antibody formation; biopharmaceuticals; blood donors; blood serum; cosmetics; enzyme-linked immunosorbent assay; humans; immunoglobulin G; immunoglobulin M; moieties; polyethylene glycol; precipitin tests; processed foods; screening; seroprevalence; therapeutics
Abstract:
... Detection of anti-drug antibodies is a critical step in the development of large molecule biopharmaceuticals. In the case of multicomponent/multifunctional molecules, such as fusion proteins and protein conjugates such as covalent polyethylene glycol (PEG)~protein conjugates, it is useful to further characterize anti-drug antibody (ADA) binding to key domains of the drug. The detection of anti-PEG ...
DOI:
10.1016/j.jim.2019.112669

44. A gold nanoparticle strip for simultaneously evaluating FMDV immunized antibody level and discriminating FMDV vaccinated animals from infected animals

Author:
Suzhen Yang; Yaning Sun; Jifei Yang; Yunchao Liu; Hua Feng; Gaiping Zhang
Source:
RSC advances 2019 v.9 no.52 pp. 30164-30170
ISSN:
2046-2069
Subject:
antibodies; enzyme-linked immunosorbent assay; epitopes; goats; immunoglobulin G; nanogold; staphylococcal protein A; structural proteins; viral nonstructural proteins; viruses
Abstract:
... A gold nanoparticle strip was developed for rapidly evaluating FMDV type O antibody level and simultaneously discriminating FMDV vaccinated animals from infected animals. The strip was established depending on the colloidal gold nanoparticle labeling technique. Staphylococcal protein A colloidal gold nanoparticles were used as a probe. The epitope antigens of FMDV structural proteins and nonstruct ...
DOI:
10.1039/c9ra04810c

45. A hematologic key for bovine leukemia virus screening in Japanese black cattle

Author:
Masataka Akagami; Shoko Oya; Yuki Kashima; Satoko Seki; Yoshinao Ouchi; Yoko Hayama
Source:
Journal of veterinary diagnostic investigation 2019 v.31 no.4 pp. 568-571
ISSN:
1943-4936
Subject:
Bovine leukemia virus; antibodies; blood sampling; confidence interval; dairy cattle; enzootic bovine leukosis; enzyme-linked immunosorbent assay; graphs; lymphocyte count; quantitative polymerase chain reaction; screening
Abstract:
... The European Community’s leukosis key (EC key) is a well-known hematologic method for detecting bovine leukemia virus (BLV) infection in dairy cattle. The key identifies infected cattle with persistent lymphocytosis via a combination of lymphocyte count (LC) and age. Using the EC key to identify BLV-infected Japanese black (JB) cattle is problematic, however, given the inherently lower LCs of JB c ...
DOI:
10.1177/1040638719848449
PubMed:
31046652
PubMed Central:
PMC6857024

46. A high-sensitivity enzyme immunoassay for the quantification of soluble human semaphorin 4D in plasma

Author:
Anna Laber; Elisabeth Gadermaier; Jacqueline Wallwitz; Gabriela Berg; Gottfried Himmler
Source:
Analytical biochemistry 2019 v.574 pp. 15-22
ISSN:
0003-2697
Subject:
EDTA (chelating agent); blood coagulation; blood serum; cell communication; citrates; enzyme activity; enzyme-linked immunosorbent assay; epitopes; heparin; humans; membrane glycoproteins; monoclonal antibodies; platelet activation; proteinase inhibitors
Abstract:
... Human semaphorin 4D (SEMA4D), a type I integral membrane glycoprotein, regulates key cellular functions (e.g. cell-cell communication, platelet activation). Its 120 kDa extracellular region can be shed from the membrane to release soluble SEMA4D (sSEMA4D). Studies on circulating sSEMA4D levels are mostly performed with poorly characterized assays and use serum and plasma as matrix.We developed and ...
DOI:
10.1016/j.ab.2019.03.004

47. A high-throughput inhibition assay to study MERS-CoV antibody interactions using image cytometry

Author:
Osnat Rosen; Leo Li-Ying Chan; Olubukola M. Abiona; Portia Gough; Lingshu Wang; Wei Shi; Yi Zhang; Nianshuang Wang; Wing-Pui Kong; Jason S. McLellan; Barney S. Graham; Kizzmekia S. Corbett
Source:
Journal of virological methods 2019 v.265 pp. 77-83
ISSN:
0166-0934
Subject:
Middle East respiratory syndrome coronavirus; enzyme-linked immunosorbent assay; flow cytometry; interferometry; monoclonal antibodies; neutralization; neutralizing antibodies; pathogens; proteins; public health; screening; therapeutics; vaccines; virion
Abstract:
... The emergence of new pathogens, such as Middle East respiratory syndrome coronavirus (MERS-CoV), poses serious challenges to global public health and highlights the urgent need for methods to rapidly identify and characterize potential therapeutic or prevention options, such as neutralizing antibodies. Spike (S) proteins are present on the surface of MERS-CoV virions and mediate viral entry. S is ...
DOI:
10.1016/j.jviromet.2018.11.009
PubMed:
30468747
PubMed Central:
PMC6357230

48. A laboratory based exposure of Microcystis and Oscillatoria cyanobacterial isolates to heterotrophic bacteria

Author:
L.L. Ndlela; P.J. Oberholster; J.H. Van Wyk; P.H. Cheng
Source:
Toxicon 2019 v.165 pp. 1-12
ISSN:
0041-0101
Subject:
Microcystis; Oscillatoria; alkaline phosphatase; bacteria; biological control; chlorophyll; cyanobacterial toxins; enzyme activity; enzyme-linked immunosorbent assay; fluorescence; fluorescence microscopy; hydrochemistry; predation; scanning electron microscopy
Abstract:
... Biological control of cyanobacteria is a viable means of controlling nuisance bloom occurrences; however the majority of studies done are against Microcystis sp., with a commonly lytic effect caused. Filamentous cyanobacteria such as Oscillatoria are not as extensively studied in this area of biological control and are often part of Microcystis dominated blooms. This study employed heterotrophic b ...
DOI:
10.1016/j.toxicon.2019.04.002

49. A lateral flow assay for the rapid diagnosis of Mycobacterium bovis infection in wild boar

Author:
Alba Fresco‐Taboada; María A. Risalde; Christian Gortázar; Istar Tapia; Isabel González; Ángel Venteo; Antonio Sanz; Paloma Rueda
Source:
Transboundary and emerging diseases 2019 v.66 no.5 pp. 2175-2179
ISSN:
1865-1674
Subject:
Mycobacterium bovis; Mycobacterium tuberculosis; Sus scrofa; adults; animal diseases; antibodies; antigens; bacteria; blood serum; chronic diseases; diagnostic techniques; enzyme-linked immunosorbent assay; etiological agents; feral animals; juveniles; monitoring; pets; point-of-care systems; rapid methods; screening; swine; tuberculosis; wild boars; wildlife
Abstract:
... The native Eurasian wild boar (Sus scrofa) is a reservoir of Mycobacterium bovis, the causative agent of animal tuberculosis (TB), a chronic disease in livestock, companion animals and wild mammals. Cases of M. bovis infection in wild boar or feral pig have been reported worldwide, making early detection a priority in the eradication of the disease. Point‐of‐care diagnostic tests, such as low cost ...
DOI:
10.1111/tbed.13260

50. A lipid-soluble extract of Pinellia pedatisecta Schott enhances antitumor T cell responses by restoring tumor-associated dendritic cell activation and maturation

Author:
Yumeng Wang; Haixia Huang; Sheng Yao; Guiling Li; Congjian Xu; Yang Ye; Suiqi Gui
Source:
Journal of ethnopharmacology 2019 v.241 pp. 111980
ISSN:
0378-8741
Subject:
CD8-positive T-lymphocytes; Oriental traditional medicine; Pinellia; Western blotting; blood serum; cytotoxicity; dendritic cells; drugs; drying; enzyme-linked immunosorbent assay; flow cytometry; gene expression regulation; immunity; in vitro studies; in vivo studies; interferon-gamma; interleukin-12; lymph nodes; major histocompatibility complex; mice; models; non-specific protein-tyrosine kinase; secretion; spleen; tumor necrosis factor-alpha
Abstract:
... Pinellia pedatisecta Schott (PPS)is a traditional Chinese medicine functioning as reducing swelling and drying dampness. Pinellia pedatisecta Schott extract (PE) has been confirmed to suppress cervical tumor growth and modulate the antitumor CD4+T helper immunity towards Th1.To explore the roles of PE in modulating tumor-associated dendritic cell (TADC) activation and function.For in vivo studies, ...
DOI:
10.1016/j.jep.2019.111980