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You searched for: Publication year rev 7981-2019 Remove constraint Publication year rev: 7981-2019 Subject term immobilized enzymes Remove constraint Subject term: immobilized enzymes Subject term peptides Remove constraint Subject term: peptides
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1. Biotransformation of Isoeugenol into Vanillin Using Immobilized Recombinant Cells Containing Isoeugenol Monooxygenase Active Aggregates

Author:
Liqing Zhao; Yingzi Jiang; Huiyan Fang; Haichao Zhang; Shuang Cheng; Muhammad Shahid Riaz Rajoka; Yiguang Wu
Source:
Applied biochemistry and biotechnology 2019 v.189 no.2 pp. 448-458
ISSN:
0273-2289
Subject:
Escherichia coli; bacteria; biotransformation; buffers; dimethyl sulfoxide; glutaraldehyde; immobilized enzymes; pH; peptides; vanillin
Abstract:
... For efficiently enhancing the activity of isoeugenol monooxygenase, a whole cell overproducing active aggregate IEM720-18A was successfully fabricated via the fusion of amphiphilic short peptide 18A (EWLKAFYEKVLEKLKELF) and isoeugenol monooxygenase and then efficiently expressed in E. coli BL21 (DE3). Such resulting bacteria, E. coli BL21 (DE3) harboring pET30a-IEM720-18A was applied in the biotra ...
DOI:
10.1007/s12010-019-02996-1

2. Continuous preparation and characterization of immunomodulatory peptides from type II collagen by a novel immobilized enzyme membrane reactor with improved performance

Author:
Hui Cao; Jifang Cao; Yujun Zhang; Tai Ye; Jin Song Yu; Min Yuan; Fei Xu; Wenxin Zheng; Xiaojia Zuo
Source:
Journal of food biochemistry 2019 v.43 no.7 pp. e12862
ISSN:
0145-8884
Subject:
binding capacity; byproducts; cartilage; chicks; chronic diseases; collagen; enzymatic hydrolysis; functional foods; half life; hydrolysates; hydrolysis; immobilized enzymes; immunomodulators; leukocytes; lymphocyte proliferation; meat processing; molecular weight; peptides; proteinases; wastes
Abstract:
... In this study, a novel method of continuous coupling of immobilized enzymatic hydrolysis reactor and membrane separation (CIEH‐MS) was used to isolate the immunomodulatory peptides from type II collagen (CII) in chick sternal cartilage. The immobilized neutral protease was successfully prepared with an activity of 400.5 U/g. The CIEH‐MS system loaded with immobilized neutral protease had high oper ...
DOI:
10.1111/jfbc.12862

3. Display of Escherichia coli Phytase on the Surface of Bacillus subtilis Spore Using CotG as an Anchor Protein

Author:
Sirima Mingmongkolchai; Watanalai Panbangred
Source:
Applied biochemistry and biotechnology 2019 v.187 no.3 pp. 838-855
ISSN:
0273-2289
Subject:
Bacillus subtilis; Escherichia coli; Western blotting; bioactive properties; enzyme activity; immobilized enzymes; pH stability; pepsin; peptides; phytases; spores; thermal stability; yeasts
Abstract:
... Escherichia coli phytase (AppA) has been widely used as an exogenous feed enzyme for monogastric animals; however, the production of this enzyme has been examined primarily in E. coli and yeast expression systems. As an alternative to production of soluble phytase, an enzyme immobilization method using the Bacillus subtilis spore outer-coat protein CotG as an anchoring motif for the display of the ...
DOI:
10.1007/s12010-018-2855-7

4. Immobilization of Alkaline Collagenase from Bacillus subtilis onto Sulfonated Polystyrene Nanospheres for Hydrolysis of Tilapia Collagen

Author:
Ling Zhang; Xiaocui Yang; Kaijun Xiao; Yuyi Lu; Chunhai Li; Zijin Zhang
Source:
Journal of food quality 2019 v.2019 pp. -
ISSN:
0146-9428
Subject:
Bacillus subtilis; Fourier transform infrared spectroscopy; amino acid sequences; collagen; collagenase; energy-dispersive X-ray analysis; hydrolysis; immobilized enzymes; industrial applications; liquid chromatography; molecular weight; nanoparticles; nanospheres; pH; particle size; particle size distribution; peptides; polystyrenes; scanning electron microscopy; subtilisin; tandem mass spectrometry; temperature; transmission electron microscopy
Abstract:
... The structure of an alkaline protease from Bacillus subtilis used by a tilapia collagen peptide manufacturer was analyzed, and the technology of the enzyme immobilized by sulfonated polystyrene (SPS) nanoparticles was studied. The particle size distribution, SEM, EDS, TEM, and FT-IR spectroscopy of the carrier before and after immobilization were analyzed. The results showed that the molecular wei ...
DOI:
10.1155/2019/7521895

5. Proteolytic ceramic capillary membranes for the production of peptides under flow

Author:
Marieke M. Hoog Antink; Tim Sewczyk; Stephen Kroll; Pál Árki; Sascha Beutel; Kurosch Rezwan; Michael Maas
Source:
Biochemical engineering journal 2019 v.147 pp. 89-99
ISSN:
1369-703X
Subject:
carboxylation; casein; ceramics; electrostatic interactions; hydrolysis; immobilized enzymes; leaching; models; peptides; proteinases; proteolysis
Abstract:
... In this study, we investigate the effect of membrane surface functionalization on the immobilization of the protease subtilisin A and its performance in the production of peptides from the model protein casein under flow. The surface of tubular ceramic membranes was silanized to yield carboxylated and aminated supports for enzyme immobilization via non-covalent and carbodiimide activated binding. ...
DOI:
10.1016/j.bej.2019.04.005

6. Recent advances in metal-organic frameworks for separation and enrichment in proteomics analysis

Author:
Qianjing Liu; Nianrong Sun; Chun-hui Deng
Source:
Trends in analytical chemistry 2019 v.110 pp. 66-80
ISSN:
0165-9936
Subject:
adsorbents; binding sites; chemical species; coordination polymers; immobilized enzymes; ligands; peptides; proteins; proteomics; surface area
Abstract:
... Metal-organic frameworks (MOFs) have been broadly applied to sample preparation which is an essential step in proteomics research. Their large surface area provides abundant binding sites, their specific pore structure can exclude large-sized proteins and allow small molecules to get inside their channels. Besides, the flexible functionalization gives infinite possibilities of MOFs applications in ...
DOI:
10.1016/j.trac.2018.10.033

7. Unambiguous Sequence Characterization of a Monoclonal Antibody in a Single Analysis Using a Nonspecific Immobilized Enzyme Reactor

Author:
Joshua D. Hinkle; Robert A. D’Ippolito; Maria C. Panepinto; Wei-Han Wang; Dina L. Bai; Jeffrey Shabanowitz; Donald F. Hunt
Source:
Analytical chemistry 2019 v.91 no.21 pp. 13547-13554
ISSN:
1520-6882
Subject:
aspergillopepsin; batch systems; chromatography; computer software; dissociation; electron transfer; immobilized enzymes; monoclonal antibodies; peptides; pharmaceutical industry; sequence analysis; therapeutics
Abstract:
... Accurate sequence characterization is essential for the development of therapeutic antibodies by the pharmaceutical industry. Presented here is a methodology to obtain comprehensive sequence analysis of a monoclonal antibody. An enzyme reactor of immobilized Aspergillopepsin I, a highly stable nonspecific protease, was used to cleave reduced antibody subunits into a peptide profile ranging from 1 ...
DOI:
10.1021/acs.analchem.9b02666