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1. In-depth site-specific O-Glycosylation analysis of therapeutic Fc-fusion protein by electron-transfer/higher-energy collisional dissociation mass spectrometry

Author:
Hashii, Noritaka,   et al.   ; Suzuki, Junya; Hanamatsu, Hisatoshi; Furukawa, Jun-ichi; Ishii-Watabe, Akiko;     Show all 5 Authors
Source:
Biologicals 2019 v.58 pp. 35-43
ISSN:
1045-1056
Subject:
biopharmaceuticals; consensus sequence; databases; dissociation; glucagon-like peptide 1; glycosylation; liquid chromatography; mammals; mass spectrometry; peptides; proteins; therapeutics
Abstract:
... Unexpected O-glycosylations, including O-xylosylations and mucin-type O-glycosylations, have been reported in recent glycosylation analyses of Fc-fusion proteins produced in mammalian cell expression systems. This observation suggests that therapeutic proteins with novel structures can undergo unintended O-glycosylations, having implications regarding their efficacy and safety. Therefore, the impl ...
DOI:
10.1016/j.biologicals.2019.01.005