U.S. flag

An official website of the United States government

PubAg

Main content area

You searched for: Publication Year 2019 Remove constraint Publication Year: 2019 Subject mass spectrometry Remove constraint Subject: mass spectrometry Subject acetylation Remove constraint Subject: acetylation
Start Over

Search

15  Search Results

1 - 15 of 15
Number of results to display per page

Search Results

1. Lysine acetylation contributes to development, aflatoxin biosynthesis and pathogenicity in Aspergillus flavus

Author:
Yang, Guang; Yue, Yuewei; Ren, Silin; Yang, Mingkun; Zhang, Yi; Cao, Xiaohong; Wang, Yinchun; Zhang, Jia; Ge, Feng; Wang, Shihua
Source:
Environmental microbiology 2019 v.21 no.12 pp. 4792-4807
ISSN:
1462-2912
Subject:
Aspergillus flavus; acetylation; aflatoxins; antibodies; biosynthesis; carcinogenicity; conidia; crops; enzyme activity; gluconeogenesis; glycolysis; lysine; mass spectrometry; methyltransferases; mutation; pathogenicity; pentose phosphate cycle; peptides; post-translational modification; proteins; sclerotia; tricarboxylic acid cycle; virulent strains
Abstract:
... Aspergillus flavus is a pathogenic fungus that produces carcinogenic aflatoxins, posing a great threat to crops, animals and humans. Lysine acetylation is one of the most important reversible post‐translational modifications and plays a vital regulatory role in various cellular processes. However, current information on the extent and function of lysine acetylation and aflatoxin biosynthesis in A. ...
DOI:
10.1111/1462-2920.14825

2. A target‐group‐change couple with mass defect filtering strategy to identify the metabolites of “Dogel ebs” in rats plasma, urine and bile

Author:
Dong, Xin; Li, XiaoNa; Li, Na; Zhao, HongMei; GuLa, A; Zhang, Xuan; Zhang, Ping; Bao, BaoQuan
Source:
Journal of separation science 2019 v.42 no.21 pp. 3382-3389
ISSN:
1615-9306
Subject:
Sophora flavescens; acetylation; alkaloids; bile; biochemical pathways; chemical composition; decarboxylation; demethylation; dysentery; flavonoids; herbal medicines; high performance liquid chromatography; mass spectrometry; metabolites; oxidation; rats; toxicity; urine
Abstract:
... “Dogel ebs” was known as Sophora flavescens Ait., a classical traditional Chinese Mongolian herbal medicine, which had the effects on damp‐heat dysentery, scrofula, and syndrome of accumulated dampness toxicity. Although the chemical constituents have been clarified by our previous studies, the metabolic transformation of “Dogel ebs” in vivo was still unclear. To explore the mechanism of “Dogel eb ...
DOI:
10.1002/jssc.201900466

3. Comprehensive Study of the in Vivo and in Vitro Metabolism of Dietary Isoflavone Biochanin A Based on UHPLC-Q-TOF-MS/MS

Author:
Yin, Jintuo; Zhang, Xiaowei; Zhang, Yuqian; Ma, Yinling; Li, Luya; Li, Deqiang; Zhang, Lantong; Zhang, Zhiqing
Source:
Journal of agricultural and food chemistry 2019 v.67 no.45 pp. 12481-12495
ISSN:
1520-5118
Subject:
acetylation; biochanin A; biotransformation; demethylation; glucose; hydrogenation; hydrolysis; intestinal microorganisms; liver microsomes; mass spectrometry; metabolites; methylation; oxidation; rats; toxicity; ultra-performance liquid chromatography
Abstract:
... Biochanin A is a dietary isoflavone with multiple biological functions. Owing to a lack of comprehensive studies of biochanin A metabolism, this study was designed to further clarify the processes involved in biochanin A metabolism. In this study, ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS/MS) was utilized to characterize th ...
DOI:
10.1021/acs.jafc.9b05776

4. Flavonol triglycosides from Ornithopus compressus L. (Fabaceae)

Author:
Scharenberg, Friederike; Çiçek, Serhat Sezai; Stegemann, Thomas; Piccolella, Simona; Pacifico, Severina; Zidorn, Christian
Source:
Industrial crops and products 2019 v.137 pp. 475-483
ISSN:
0926-6690
Subject:
2,2-diphenyl-1-picrylhydrazyl; Ornithopus compressus; acetylation; aerial parts; cations; cytotoxicity; glycosides; hydrolysis; isorhamnetin; kaempferol; mass spectrometry; nuclear magnetic resonance spectroscopy; quercetin; sugars
Abstract:
... Five flavonoid glycosides were isolated from the aerial parts of Ornithopus compressus L., yellow serradella (Fabaceae). The structures of the isolated natural products were determined as kaempferol 3-O-(2,6-di-O-β-D-glucopyranosyl)-β-D-galactopyranoside (1), kaempferol 3-O-β-D-glucopyranosyl-(1→6)-[2-O-(2E)-feruloyl-β-glucopyranosyl]-(1→2)-β-D-galactopyranoside (2), quercetin 3-O-(2,6-di-O-β-D-gl ...
DOI:
10.1016/j.indcrop.2019.05.048

5. Gypsophila saponins enhance the cytotoxicity of etoposide in HD-MY-Z lymphoma cells

Author:
Gevrenova, Reneta; Zaharieva, Maya M.; Kroumov, Alexander D.; Voutquenne-Nazabadioko, Laurence; Zheleva-Dimitrova, Dimitrina; Balabanova, Vessela; Hajdenski, Hristo M.; Konstantinov, Spiro
Source:
Food and chemical toxicology 2019
ISSN:
0278-6915
Subject:
Gypsophila; Hodgkin disease; acetylation; apoptosis; arabinose; carboxylic acids; caspases; cell lines; computer software; cytotoxicity; desmosides; etoposide; fibroblasts; inhibitory concentration 50; liquid chromatography; mass spectrometry; mice; neoplasm cells; root extracts; sulfates; synergism; triterpenoid saponins
Abstract:
... Glucuronide Oleanane-type Triterpenoid Carboxylic Acid 3,28-Bidesmosides (GOTCAB) are accumulated in Gypsophila L. roots. In the study we aimed at investigating the possible synergistic effects of Gypsophila trichotoma GOTCABs and cytostatic etoposide towards the Hodgkin lymphoma cell line HD-MY-Z. The combination effects with etoposide were evaluated using the symbolic mathematical software MAPLE ...
DOI:
10.1016/j.fct.2019.110777

6. An available strategy based on accurate mass by ultra high performance liquid chromatography coupled to Fourier transform ion cyclotron resonance mass spectrometry technology to characterization of metabolic profile of palbociclib in rat urine, feces and bile

Author:
Yao, Jiaxin; Jiang, Xu; Liu, Qiao; Yuan, Xuemei; Feng, Tiantian; Li, Kunjie; Zhao, Longshan
Source:
Journal of chromatography 2019 v.1124 pp. 37-46
ISSN:
1570-0232
Subject:
acetylation; bile; biochemical pathways; breast neoplasms; cyclin-dependent kinase; deamination; dehydrogenation; feces; hydroxylation; mass spectrometry; metabolism; metabolites; oxidation; rats; sulfates; ultra-performance liquid chromatography; urine
Abstract:
... Palbociclib (named PD 0332991) is a novel highly selective cyclin-dependent kinase 4 and 6 (CDK 4/6) inhibitor, which has been approved by the Food and Drug Administration (FDA) for the treatment of hormone-receptor-positive advanced breast cancer. This present study developed a comprehensive strategy to investigate the metabolic profile of palbociclib in rat urine, feces and bile samples based on ...
DOI:
10.1016/j.jchromb.2019.05.032

7. Influence of microorganism and plant oils on the structure of mannosylerythritol lipid (MEL) biosurfactants revealed by a novel thin layer chromatography mass spectrometry method

Author:
Beck, Alexander; Haitz, Fabian; Grunwald, Saskia; Preuss, Laura; Rupp, Steffen; Zibek, Susanne
Source:
Journal of industrial microbiology & biotechnology 2019 v.46 no.8 pp. 1191-1204
ISSN:
1367-5435
Subject:
Ustilaginaceae; acetylation; biosurfactants; chemical structure; coconut oil; cosmetics; detergents; fatty acids; fungi; gas chromatography; glycolipids; hydrophilicity; hydrophobicity; mass spectrometry; thin layer chromatography
Abstract:
... Mannosylerythritol lipids (MEL) are microbial glycolipid biosurfactants with great potential for application in cosmetics and household detergents. In current biotechnological processes, they are produced by basidiomycetous fungi, the Ustilaginaceae, as a complex mixture of different chemical structures. It was the aim of this paper to study the influence of producer organisms and substrates on th ...
DOI:
10.1007/s10295-019-02194-2

8. Recombinant HCV NS3 and NS5B enzymes exhibit multiple posttranslational modifications for potential regulation

Author:
Hernández, Sergio; Díaz, Ariel; Loyola, Alejandra; Villanueva, Rodrigo A.
Source:
Virus genes 2019 v.55 no.2 pp. 227-232
ISSN:
0920-8569
Subject:
Baculoviridae; Hepatitis C virus; acetylation; amino acids; enzymes; functional diversity; insects; mass spectrometry; phosphorylation; polypeptides; recombinant proteins; ubiquitination
Abstract:
... Posttranslational modification (PTM) of proteins is critical to modulate protein function and to improve the functional diversity of polypeptides. In this report, we have analyzed the PTM of both hepatitis C virus NS3 and NS5B enzyme proteins, upon their individual expression in insect cells under the baculovirus expression system. Using mass spectrometry, we present evidence that these recombinan ...
DOI:
10.1007/s11262-019-01638-2

9. Identification of the Functional Roles of Six Key Proteins in the Biosynthesis of Enterobacteriaceae Colanic Acid

Author:
Scott, Phillip M.; Erickson, Katelyn M.; Troutman, Jerry M.
Source:
Biochemistry 2019 v.58 no.13 pp. 1818-1830
ISSN:
1520-4995
Subject:
Escherichia coli; acetylation; acetyltransferases; biosynthesis; fluorescence; fucose; galactose; gene overexpression; glucose; glucuronic acid; high performance liquid chromatography; isoprenoids; mass spectrometry; operon; pH; polymers; proteins
Abstract:
... When subjected to harsh conditions such as low pH, pathogenic Escherichia coli can secrete colanic acid to establish a protective barrier between the organism and the acidic environment. The colanic acid consists of a six-sugar repeating unit polymer comprised of glucose, fucose, galactose, and glucuronic acid. The region of the E. coli genome that encodes colanic acid biosynthesis has been report ...
DOI:
10.1021/acs.biochem.9b00040
PubMed:
30821147
PubMed Central:
PMC6445743

10. Using Peptide Arrays To Discover the Sequence-Specific Acetylation of the Histidine-Tyrosine Dyad

Author:
Szymczak, Lindsey C.; Mrksich, Milan
Source:
Biochemistry 2019 v.58 no.13 pp. 1810-1817
ISSN:
1520-4995
Subject:
acetic anhydride; acetylation; amino acid sequences; desorption; mass spectrometry; peptides; proteins
Abstract:
... Reactions that can selectively modify amino acid sequences within peptides and proteins are important for preparing protein reagents, immobilizing proteins, and making antibody-drug conjugates. The development of new reactions often begins with known chemistries and optimizes yields using a small set of peptide reactants. This article describes the use of peptide arrays and self-assembled monolaye ...
DOI:
10.1021/acs.biochem.9b00022

11. A high glucose level is associated with decreased aspirin-mediated acetylation of platelet cyclooxygenase (COX)-1 at serine 529: A pilot study

Author:
Finamore, Francesco; Reny, Jean-Luc; Malacarne, Sarah; Fontana, Pierre; Sanchez, Jean-Charles
Source:
Journal of proteomics 2019 v.192 pp. 258-266
ISSN:
1874-3919
Subject:
acetylation; aspirin; blood platelets; cardiovascular diseases; diabetes; glucose; glycation; hyperglycemia; mass spectrometry; patients; prostaglandin synthase; proteins; risk factors; serine
Abstract:
... Diabetes is a major risk factor for cardiovascular diseases. Although aspirin is considered a cornerstone of the prevention and treatment of atherothrombotic-related ischemic events, this antiplatelet drug appears to be less effective in patients with poorly controlled diabetes. It has been suggested that the glycation of platelet proteins plays a pivotal role in poor responsiveness to aspirin. Ho ...
DOI:
10.1016/j.jprot.2018.09.007

12. Enzymatic Production and Enzymatic-Mass Spectrometric Fingerprinting Analysis of Chitosan Polymers with Different Nonrandom Patterns of Acetylation

Author:
Wattjes, Jasper; Niehues, Anna; Cord-Landwehr, Stefan; Hoßbach, Janina; David, Laurent; Delair, Thierry; Moerschbacher, Bruno M.
Source:
Journal of the American Chemical Society 2019 v.141 no.7 pp. 3137-3145
ISSN:
1520-5126
Subject:
Pestalotiopsis; Podospora anserina; Puccinia graminis; acetylation; bioactive properties; biopolymers; chemical analysis; chitin; chitosan; enzymes; fungi; mass spectrometry; nuclear magnetic resonance spectroscopy; polymerization
Abstract:
... Chitosans, a family of ß-(1,4)-linked, partially N-acetylated polyglucosamines, are considered to be among the most versatile and most promising functional biopolymers. Chemical analysis and bioactivity studies revealed that the functionalities of chitosans strongly depend on the polymers’ degree of polymerization and fraction of acetylation. More recently, the pattern of acetylation (PA) has been ...
DOI:
10.1021/jacs.8b12561

13. Site-Selective Lysine Reactions Guided by Protein–Peptide Interaction

Author:
Zhang, Yu; Liang, Yujie; Huang, Feng; Zhang, Yue; Li, Xuechen; Xia, Jiang
Source:
Biochemistry 2019 v.58 no.7 pp. 1010-1018
ISSN:
1520-4995
Subject:
acetylation; hydroxylation; isothiocyanates; lysine; mammals; mass spectrometry; methylation; peptides; post-translational modification; proteome; ubiquitins
Abstract:
... Site-selective lysine post-translational modifications such as acetylation, methylation, hydroxylation, and isopeptide formation mediate the precise control of important signaling events in cells with unmistakable accuracy. This unparalleled site selectivity (modification of a single lysine in a particular protein in the proteome) is still a challenge for non-enzymatic protein reactions; the diffi ...
DOI:
10.1021/acs.biochem.8b01223

14. Modified metabolites mapping by liquid chromatography-high resolution mass spectrometry using full scan/all ion fragmentation/neutral loss acquisition

Author:
Li, Hua; Qin, Qian; Shi, Xianzhe; He, Jun; Xu, Guowang
Source:
Journal of chromatography 2019 v.1583 pp. 80-87
ISSN:
0021-9673
Subject:
acetylation; biomarkers; breast neoplasms; data collection; false positive results; liquid chromatography; mass spectrometry; metabolites; monitoring; patients; physiological regulation; urine
Abstract:
... Modified metabolites play important roles in diagnostic monitoring, oxidative response and physiological regulation. Comprehensive analytical methods are greatly needed for improving the coverage of modified metabolites and studying their physiological function. Here, a novel nontargeted profiling method for mapping modified metabolites was developed by liquid chromatography-high resolution mass s ...
DOI:
10.1016/j.chroma.2018.11.014

15. Metabolism and mutual biotransformations of anthraquinones and anthrones in rhubarb by human intestinal flora using UPLC-Q-TOF/MS

Author:
Huang, Zhenghai; Xu, Yang; Wang, Qing; Gao, Xiaoyan
Source:
Journal of chromatography 2019 v.1104 pp. 59-66
ISSN:
1570-0232
Subject:
acetylation; bioactive properties; biochemical pathways; biotransformation; cleavage (chemistry); demethylation; emodin; energy; humans; hydrolysis; hydroxylation; intestinal microorganisms; mass spectrometry; metabolites; methylation; oxidation; rhubarb; ultra-performance liquid chromatography
Abstract:
... Anthraquinones and anthrones are the main active components of rhubarb. To investigate the metabolism and possible mutual biotransformations pathways of anthraquinones and anthrones by human intestinal flora, 9 representative constituents (aloe-emodin, rhein, emodin, chrysophanol, physcion, sennosides A, B, C and D) were studied. An ultra-performance liquid chromatography coupled with quadrupole t ...
DOI:
10.1016/j.jchromb.2018.10.008