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Zingiber zerumbet; arithmetics; camphene; camphor; cineole; climatic factors; cluster analysis; essential oils; gas chromatography-mass spectrometry; genetic variation; genotype; germplasm; microsatellite repeats; oils; principal component analysis; rhizomes; volatile compounds; India
Abstract:
... Essential oil of twenty-five germplasms of Zingiber zerumbet from different accessions of Eastern India were analysed using Gas Chromatography-Mass Spectroscopy (GC/MS). A total of 29 constituents were identified representing between 72.45-98.93% of total area. Zerumbone content was found to be in a range of 60.1-79.59% for the tested rhizomes. Acc. Haroa (W.B) was found to contain highest amount ...
... The planting of Pinus elliottii Engelm. has now reached close to three million ha in China. Molecular breeding as part of the improvement program for P. elliottii in southern China has been carried out in recent years. Third-generation sequencing (Pacbio sequencing technology, TGS) was used to obtain the exome of P. elliottii for molecular breeding. A total of 35.8 Gb clean reads were generated us ...
... Apple mildew, caused by the fungus Podosphaera leucotricha, is an ongoing problem for apple growers in most world production regions. To manage apple foliar mildew with less fungicide, use of mildew-resistant cultivars is an ideal option. The mildew resistance locus of crabapple ‘White Angel’ was previously named Pl-w and genetically mapped, with the closest DNA markers at least 4.6 cM away on one ...
Sillago sihama; aquaculture; cytosine; economic valuation; frequency distribution; genome; genomics; guanine; heterozygosity; high-throughput nucleotide sequencing; microsatellite repeats; surveys; China
Abstract:
... Sillago sihama has high economic value and is one of the most attractive aquaculture species in China. Despite its economic importance, studies of its genome have barely been performed. In this study, we conducted a first genomic survey of S. sihama using next-generation sequencing (NGS). In total, 45.063 Gb of high-quality sequence data were obtained. For the 17-mer frequency distribution, the ge ...
... Microsatellite markers were used to investigate the reproductive behavior of the damselfish Abudefduf sordidus at Johnston Atoll, Central Pacific Ocean. Genetic results indicated that ten males maintained guardianship over their nest territories for up to nine nest cycles during a 3.5 month period. Genotypes of 1025 offspring sampled from 68 nests (composed of 129 clutches) were consistent with 95 ...
... The genetic structure of a sample of isolates of the oomycete plant pathogen Phytophthora cinnamomi from natural and agricultural outbreaks and the long-distance movement of individual genotypes were studied using four microsatellite markers to genotype 159 isolates of Californian, Mexican, and worldwide origins. Allelic profiles identified 75 multilocus genotypes. A STRUCTURE analysis placed them ...
... Genetic profiling has been used to link mosquito bloodmeals to the individual humans, but this analysis has not been done for other mammalian bloodmeals. In this study, we describe a microsatellite-based method for identifying individual pigs in mosquito bloodmeals based on their unique multilocus genotypes. Eleven tetranucleotide microsatellites and a sex-specific marker were selected based on Sm ...
... Traditional methods for developing polymorphic microsatellite loci without reference sequences are time-consuming and labor-intensive, and the polymorphisms of simple sequence repeat (SSR) loci developed from expressed sequence tag (EST) databases are generally poor. To address this issue, in this study, we developed a new software (PSSRdt) and established an effective method for directly obtainin ...
... Extra virgin olive oil (EVOO) has elevated commercial value due to its health appeal, desirable characteristics and quantitatively limited production, and thus it has become an object of intentional adulteration. As EVOOs on the market might consist of a blend of olive varieties or sometimes even of a mixture of oils from different botanical species, an array of DNA-fingerprinting methods have bee ...
... Black bear distribution and dispersal in Mexico are uncertain. Here we document the occurrence of a black bear in the Sierra Gorda Biosphere Reserve in the Mexican State of Queretaro. We assessed its population membership using microsatellites genetic markers and conclude the individual came from the Nuevo Leon population, which is approximately 200 km north of the study area. ...
... Mercurialis perennis is a dioecious clonal woodland herb. Its male-biased shoot sex ratio may result from differential selection (with skewed genet sex ratio) and/or differential performance (fairly even genet sex ratio but skewed shoot sex ratio since male clones grow more vigorously or have a higher flowering propensity). To test both scenarios, a total of 1024 shoots were mapped and excavated i ...
... Landscape level species assessments are rarely available to inform conservation planning. Recent advances in multi-taxa survey techniques, however, have made them more obtainable by improving efficiency of collecting species occurrence data and genetic samples. Here, we used a multi-taxa survey technique to provide a landscape level portrait of fisher (Pekania pennanti) population status in a port ...
... Dioscorea alata is a species of yam that is widely cultivated throughout the world, including China. Nevertheless, there is very limited knowledge on this type of yam diversity in China. In the present study, we assessed the genetic diversity of Chinese greater yam by analyzing their morphology, genotype, chromosome and ploidy. 142 cultivated greater yams were collected from eight geographical reg ...
... Information on the level and distribution of genetic variation is important for conservation plan of captive population of an endangered species such as tiger and cheetah. We assayed the frequency of microsatellites in the genomic and genic sequences of wild cats (Panthera tigris, Acinonyx jubatus) and compared it with the domestic cat (Felis catus). Frequency, relative abundance and density of mi ...
... Morphological variation, genotypic stability and genetic distances among micropropagated tea [Camellia sinensis (L.) Kuntze] cv. ‘Kolkhida’ plantlets after 7 years of propagation in vitro and among plantlets regenerated from callus were compared. Fifteen individuals, randomly chosen amongst a set of 100 plantlets regenerated from callus, and ten individuals, randomly chosen from a set of 100 micro ...
Kanat Gürün; Andrzej Furman; Javier Juste; Maria J. Ramos Pereira; Jorge M. Palmeirim; Sebastien J. Puechmaille; Pavel Hulva; Primoz Presetnik; Daniela Hamidovic; Carlos Ibáñez; Ahmet Karataş; Benjamin Allegrini; Panagiotis Georgiakakis; Dino Scaravelli; Marcel Uhrin; Haris Nicolaou; Mounir R. Abi-Said; Zoltan L. Nagy; Suren Gazaryan; Raşit Bilgin
Miniopterus schreibersii; Palearctic region; Turkey (country); cytochrome b; genes; mammalogy; microsatellite repeats; mitochondria; mitochondrial DNA; phylogeography; refuge habitats; social structure; Caucasus region; Central European region; Middle East; Northern Africa
Abstract:
... Miniopterus schreibersii is a cave-dwelling bat species with a wide distribution in the western Palearctic spanning southern and central Europe, North Africa, Anatolia, the Middle East, and the Caucasus. We investigated the social structure and its effects on the genetic makeup of this species, using 10 nuclear microsatellite markers and a partial fragment of the mitochondrial cytochrome b gene. S ...
Martes flavigula; deciduous forests; family relations; genetic markers; high-throughput nucleotide sequencing; kinship; loci; microsatellite repeats; pedigree; population genetics; population structure; predators; probability; South Korea
Abstract:
... BACKGROUND: Microsatellite markers are an ideal molecular marker for population genetic studies such as population structure, pedigree, and kinship. The yellow-throated marten (Martes flavigula) is widely distributed in coniferous and deciduous forests of eastern Asia and plays the role of an indicator and umbrella species in South Korea, given the absence of top predators such as tiger and leopar ...
agricultural land; cameras; color; data collection; least squares; microsatellite repeats; models; photographs; salt content; soil color; soil salinity; soil salts; surface roughness; wastelands; watersheds; China
Abstract:
... Determining soil salt content (SSC) and soil surface roughness (SSR) rapidly and simply is important for various environmental and agricultural applications. Recently, increasing attention has been focused on the color analysis to detect soil properties. Based on the fact that soil attributes affect soil color, this study investigated the feasibility of utilizing digital camera observations as an ...
... Unisexual Ambystoma are the oldest known unisexual vertebrates and comprise a lineage of eastern North American all female salamanders that reproduce by stealing sperm from as many as five normally bisexual congeneric species. The sperm may be used to only stimulate egg development by gynogenesis but can be incorporated in the zygote to elevate the ploidy level or to replace one of the female’s ha ...
... Studies on parentage and kinship of traditional Vitis vinifera grape cultivars allow light to be shed on the likely natural breeding at the basis of the assortment evolution of cultivated grapevines in a particular area over several centuries. They therefore contribute to our understanding of the process of domestication and local development of viticulture through farmer selection and allow us to ...
... As an economically important cephalopods species, wild-caught Sepia esculenta fishery has suffered a server decline due to over-fishing and ocean environmental damage. To restore this seriously declining fishery resource, we should understand the genetic foundation and molecular mechanism of spawning, reproduction and mortal of golden cuttlefish. In this study, we generated the full-length transcr ...
... The introduction of allochthonous species represents a serious threat for the native gene pools and ecosystem biodiversity. The effect is particularly disastrous for insular biocoenoses, such as in the Tuscan archipelago, one of the most important biodiversity hotspot in the Mediterranean area. The EU tool LIFE + has funded an eradication project involving a set of allochthonous species on Pianosa ...
... The stingless bee Melipona beecheii is extensively distributed across Mexico and Central America and the only Melipona found in the Greater Antilles. The aim of this work was to establish possible continental affinities of M. beecheii from the Antilles. We compared populations from the main island (Cuba) with two clearly separated genetic lineages of M. beecheii on mainland, namely the Yucatán pen ...
... Hevea brasiliensis Müll.Arg., the rubber tree, is an important industrial crop, which is the major source of nature rubber. Recently, Simple Sequence Repeat (SSR)- and Single Nucleotide Polymorphism (SNP)-based genetic maps have been reported, however further trait-related markers are needed for efficient molecular breeding in rubber tree. In this study, a total of 206 F1 progeny derived from a cr ...
White Leghorn; alleles; autosomes; body weight; chickens; developmental stages; eggs; epistasis; females; genetic markers; growth traits; loci; meat; microsatellite repeats; phenotype; quantitative trait loci
Abstract:
... Since the growth traits of chickens are largely related to the production of meat and eggs, it is definitely important to understand genetic basis of growth traits. Although many quantitative trait loci (QTLs) that affect growth traits have recently been reported in chickens, little is known about genetic architecture of growth traits across all growth stages. Therefore, we conducted a longitudina ...
Lecanosticta acicola; Pinus sylvestris; anthropogenic activities; asexual reproduction; clones; conifer needles; fungi; genetic variation; invasive species; microsatellite repeats; pathogens; population genetics; population structure; sexual reproduction; Estonia; Northern European region
Abstract:
... For northern Europe Lecanosticta acicola is an emerging pine needle pathogen. This study gives a first look into the population genetics of the pathogen in Estonia, the first population documented in that region. The main aim of this study was to investigate the genetic diversity and population structure of the pathogen in this new region for the fungus. For this purpose, 104 isolates from 2010 to ...
Salvelinus fontinalis; ancestry; biodiversity; biodiversity conservation; cold-water fish; founder effect; genetic analysis; genetic variation; habitats; indigenous species; loci; microsatellite repeats; planning; population structure; population viability; watersheds; wild fish; Northeastern United States
Abstract:
... As biodiversity is transformed on a global scale, identifying and protecting populations unaffected by habitat disturbances is essential to ensuring natural genetic diversity is not irretrievably lost. Furthermore, knowledge of the interactions between endemic populations and human-introduced conspecifics is imperative to the creation of management strategies that conserve and enhance population v ...
... We investigated the genetic diversity and phylogenetic structure of Webster’s salamander (Plethodon websteri), an understudied species of conservation concern and one of the first morphologically cryptic salamander species described solely on the basis of molecular techniques. Using a combination of mitochondrial and microsatellite data, we discovered significant genetic differentiation across the ...
... This study was conducted in three vegetation successive periods (2015, 2016, and 2017) to determine ampelographic characteristics of the 36 grapevine varieties grown in the Eastern Algerian germplasm collection by a total of 108 ampelographic criteria using OIV descriptors. The data were processed by multivariate statistical procedures. A number of factors have been well identified in the classifi ...
... Plocama calabrica (L.f.) M.Backlund & Thulin var. alba Göktürk, O.D.Düşen, B.Gürcan & U. Sarpkaya variety nova is described from South-West Anatolia. The new variety grows on limestone slopes between Akpınar and Yaylapınar villages in the Çameli district in Denizli. It is closely related to P. calabrica var. calabrica, and can be readily distinguished by morphological and molecular characters from ...
... An increasing number of infections originating from probiotic use are reported worldwide, with the majority of such cases caused by the yeast Saccharomyces 'boulardii', a subtype of S. cerevisiae. Reliably linking infectious cases to probiotic products requires unequivocal genotyping data, however, these techniques are often time-consuming and difficult to implement in routine diagnostics. This le ...
... Lynch syndrome (LS) is a common cancer syndrome that is inherited in an autosomal dominant manner. Its pathogenesis is thought to be closely related to germline mutations of mismatch repair (MMR) genes such as the MLH1, MSH2, PMS2 and MSH6 genes. This study identifies a Chinese family with LS clinically diagnosed according to the Amsterdam II criteria. In these patients, immuno-histochemical stain ...
... In the Indian subcontinent, infection with Leishmania donovani can cause fatal visceral leishmaniasis. Genetic variation in L. donovani is believed to occur rapidly from environmental changes and through selective drug pressures, thereby allowing continued disease occurrence in this region. All previous molecular markers that are commonly in use multilocus microsatellite typing and multilocus sequ ...
Baryscapus servadeii; Rickettsia; Thaumetopoea pityocampa; Wolbachia; altitude; biological control; defoliating insects; demography; eggs; forests; genetic markers; genetic variation; genotyping; global warming; homozygosity; hyperparasitoids; microsatellite repeats; population dynamics; population genetics; predators; sequence analysis; symbionts; thelytoky; Crete; Cyprus; Mediterranean region; Northern Africa; Western European region
Abstract:
... The pine processionary moth Thaumetopoea pityocampa – T. wilkinsoni is one of the main defoliators of pine and cedar forests in the Mediterranean area. Its distribution is currently expanding northward and to higher altitudes due to climate warming. This species is associated with a rich community of predators and parasitoids. The specialist egg parasitoid Baryscapus servadeii is among the most ab ...
Elaeocarpus; altitude; blueberries; botany; genetic variation; latitude; microsatellite repeats; population structure; refuge habitats; trees; Australia
Abstract:
... The wet forests of south-east Australia can be coarsely divided into cool-temperate communities characterised by relictual Gondwanan lineages with a history of persistence in scattered refugia, and subtropical communities showing stronger Malesian floristic influences, with post-glacial expansion dynamics. Insights into the assembly and dynamics of these two biomes can be gained by studying taxa t ...
... Efficient in vitro regeneration system was well-established in Polianthes tuberosa L., an attractive ornamental and aromatic plant of horticultural importance. Various explants (Crown explants, leaves, petals, flowering stems and bulb scales) and different plant growth regulators (PGRs) were evaluated for both callus induction and shoot regeneration. Results showed that the maximum callus formatio ...
... Ferula is a genus of perennial herbs belonging to the Apiaceae (Umbelliferae) family. It has 180–185 species. This genus is applied for the remedy of diverse organ disorders in folk medicine. The presence of gum-resins, sesquiterpenes, and sesquiterpene coumarins mostly characterize the genus Ferula. Ferula species have promising bioactive compounds such as auraptene, umbelliprenin, galbanic acid, ...
... The goal of this study was to identify the genetic potential of Iris germplasm in Iran and characterize it for plant breeding and conservation purposes. The genus Iris belongs to the family Iridaceae. About 300 wild species of Iris have been identified around the world. So far, 20 species and subspecies of wild irises have been discovered in Iran, which have special economic and medicinal value in ...
Zostera; anthropogenic activities; biomass; conservation areas; ecological function; genetic recombination; meadows; microrelief; microsatellite repeats; overwintering; reproduction; river deltas; seagrasses; seedlings; shoots; species recruitment; temperature; winter; China; North America; Yellow River
Abstract:
... Marine submerged aquatic angiosperms (seagrasses) are declining globally. The species Zostera japonica Asch. & Graebn. is endangered in its native range in Asia, but has been successfully introduced to North America. A large area (1031.8 ha) of Z. japonica meadow has recently been discovered in the intertidal zone of Yellow River Delta, China. This seagrass occurs along both sides of the river mou ...
... Dwarfing and semi‐dwarfing are important agronomic traits that have great potential for the improvement of wheat yields. Rht12, a dominant gibberellic acid (GA)‐responsive dwarfing gene from the gamma‐ray‐induced wheat mutant Karcagi 522M7K, is located in the long arm of chromosome 5A, which is closely linked with the locus Xwmc410. Rht12 is likely an ideal gene for GA biosynthesis and deactivatio ...
... Dispersal is a key evolutionary force affecting gene flow and genetic diversity. Therefore, analysis of population connectivity plays an important role in protecting endangered species. Ottelia acuminata var. jingxiensis is a perennial endangered aquatic plant, and present in several karst rivers of Yunnan–Guizhou Plateau. We estimated the genetic variation and population connectivity by nuclear m ...
... ‘Chenguang’ is the first released tetraploid cultivar (2n = 4x = 48) of Chinese jujube (Ziziphus jujuba Mill.) induced by colchicine. The purpose of this study was to create triploid germplasms by using ‘Chenguang’ as a parent. The controlled crosses between autotetraploid ‘Chenguang’ and diploid ‘Dongzao’ were carried out from 2013 to 2015 by maintaining the 2 parents in a nylon net and employing ...
... Activation of retrotransposon is a pivotal factor in the genesis of genetic polymorphism. Retrotransposon-based molecular markers are excellent tools for detecting genetic diversity and genomic changes associated with their activity. The objective of this study was to use IRAP markers to detect integration events of retrotransposon in Opuntia, and to compare IRAP and ISSR polymorphisms. To achieve ...
... Increasing drought conditions in Mediterranean countries are negatively impacting the survival and productivity of Castanea sativa Mill. The study aimed to select EST-SSR markers associated with drought stress developed in Quercus spp. and evaluate their transferability and polymorphism in C. sativa. Eight EST-SSR markers were selected to examine the adaptive potential of four wild populations of ...
Vulpes vulpes; ancestry; farms; foxes; fur farming; genetic analysis; genetic distance; haplotypes; microsatellite repeats; mitochondrial DNA; most probable number technique; phylogeny; phylogeography; population structure; Poland
Abstract:
... A number of studies showed that many mtDNA haplotypes were shared among contemporary farm red foxes bred on different continents and the historical wild red foxes of North American origin. Therefore, in this study, the population genetic structure and phylogeographic relationships of Polish red foxes kept on fur farms and their wild conspecifics were investigated to assess the ancestry of the farm ...
Lycaon pictus; dogs; genetic analysis; genetic variation; heterozygosity; inbreeding; microsatellite repeats; national parks; population growth; population viability; viability; wildlife management; South Africa
Abstract:
... South Africa holds a viable population of the endangered African wild dog (Lycaon pictus), with almost 500 individuals divided into (1) an unmanaged population in the Kruger National Park (KNP), (2) a free-roaming population, and (3) a managed metapopulation (MTP) that originated from reintroductions. Because metapopulation reserves are geographically isolated, translocations are ongoing to mimic ...
... Fingerprint detection and Short Tandem Repeat (STR) typing are two approaches used for identification of individuals. The main goal of forensic laboratories is the development of a standardized protocol to obtain an STR profile from latent fingerprints, by typing the DNA transferred onto touched objects. The results obtained in this field derive from studies conducted under controlled laboratory c ...
... In this study, genetic diversity of agro-morphological traits and population structure of 167 chickpea were assessed using genomic-SSR and EST-SSR molecular markers. Field evaluation of chickpea genotypes showed highly variation for most of measured characteristics such as: number of pods per plant, number of seeds per plant, 100-seed weight and seed yield. Cluster analysis based on morphological ...
... Homegardens are reservoirs of genetic resources. When native plant populations are compromised, traditional management of homegardens can be an effective means of conserving species and enhancing food security. Hylocereus undatus (dragonfruit) is a key species in homegardens but is rarely found in wild populations in the Yucatan. We analyzed management practices and genetic diversity of dragonfrui ...
... Grain Protein Content (GPC) of wheat is significant for enhanced nutritional value and is one of the most important aspects effecting pasta and bread making quality as well. Seventy seven Pakistani wheat varieties and advance lines were analysed to access the allelic distribution at microsatellite Xuhw89 locus using functional SSR marker. Overall, 42% of tested wheat genotypes were found to carry ...
Marco Dinis; Khaled Merabet; Fernando Martínez-Freiría; Sebastian Steinfartz; Miguel Vences; James D. Burgon; Kathryn R. Elmer; David Donaire; Arlo Hinckley; Soumia Fahd; Ulrich Joger; Adnane Fawzi; Tahar Slimani; Guillermo Velo-Antón
... North Africa is a climatically and topographically complex region with unique biotic assemblages resulting from the combination of multiple biogeographic realms. Here, we assess the role of climate in promoting intra-specific diversification in a Palearctic relict, the North African fire salamander, Salamandra algira, using a combination of phylogenetic and population genetic analyses, paleoclimat ...
... A comprehensive description is presented of four novel cases ofamorphus globosus (ag) foetuses originating from multiple pregnancies of Polish Holstein cows. Four amorphic foetuses were delivered by three cows. Tissue samples were collected during autopsy, embedded in paraffin, sectioned, and stained with haematoxylin and eosin. Genomic DNA was isolated from tissue samples of abnormal foetuses and ...
Osmerus mordax; coasts; data collection; genetic variation; heterozygosity; lakes; microsatellite repeats; Arctic region
Abstract:
... The genetic diversity of ten microsatellite loci is examined in samples of Arctic rainbow smelt. The expected heterozygosity estimates vary in the range of 12.6–83.9% (mean 55.3%). The degree of genetic differentiation is statistically significant: θ = 2.8%, 95% CI (1.4–4.9%). The greatest differences are found between datasets of smelt in Eastern and Western Kamchatka. The samples from Lake Nerpi ...
... Diuraphis noxia Kurdjumov, 1913 (Hemiptera: Aphididae), commonly known as the Russian wheat aphid, is a devastating pest of wheat and barley. Although fourteen sources of resistance (Dn genes) have been identified to date, none have been cloned. In this study a mapping population of 373 F₃/₄ segregating wheat plants and ‘Chinese Spring’ 7D deletion lines were screened with AFLP, SSR and EST marker ...
... The aim of this study was to evaluate the genetic variability of the White Kołuda® goose and 12 conservative flocks: Kielecka, Podkarpacka, Garbonosa, Pomerian, Rypinska, Landes, Lubelska, Suwalska, Kartuska, Romanska, Slowacka, and Kubanska, maintained in Poland using microsatellite data. The genetic diversity of geese kept in Poland remains poorly analyzed at the molecular level. In total 39 ...
... Genetic diversity studies of plants depend upon the quality and quantity of isolated DNA sample. For many lower groups of plants, apart from usual steps involved for DNA isolation, several additional steps are invariably required specific to the particular plant group. In bryophytes, nucleic acid isolation has always been a difficult task. The Bryophytes possess a substantial quantity of polypheno ...
... Diverse molecular markers have been used to analyze the genetic diversity of plant pathogens. Compared with traditional fingerprinting methods, multiple loci variable number of tandem repeat analyses (MLVAs) have gained importance recently due to their reproducibility, high discriminatory power, ease of performance, low cost, and throughput potential. These characteristics are desirable for contin ...
... Chinese hawthorn (Crataegus pinnatifida) is an important ornamental and economic horticultural plant. However, the lack of molecular markers has limited the development and utilization of hawthorn germplasm resources. Simple sequence repeats (SSRs) derived from expressed sequence tags (ESTs) allow precise and effective cultivar characterization and are routinely used for genetic diversity analysis ...
DNA; DNA fingerprinting; Phyllanthus amarus; adulterants; ecology; forensic sciences; genes; industry; melting; microsatellite repeats; models; pharmacology; rapid methods; species identification
Abstract:
... Species identification is required in various areas such as systematics, ecology, conservation, evolution, agriculture, forensics, pharmacology, food science, and even industry. Yet the number of taxonomists who identify based on morphological observations is limited, and some materials are incomplete and/or processed, leading to difficulty in species identification by morphological features. Thus ...
... In northern Japan, coastal oak forests consist of Quercus dentata (Qd) on the coastal side and Q. mongolica var. crispula (Qc) on the inland side. In the forests of northern Hokkaido, Qd is rare, and a coastal ecotype of Qc with some Qd-like traits grows on the coastal side. To reveal the genetic background of this ecotype, nuclear microsatellite genotypes in closely related oak taxa were obtained ...
... This study reinforces the argument that forest genetic resources for peripheral isolated plant populations (PIPP) need to come under rationalistic assessment, for developing and proposing conservation strategies of forest genetic resources. Cedrus brevifolia is a narrow endemic island tree occurring in a sole population. Using biparentally and paternally inherited microsatellites, the genetic cont ...
... Microsatellites are valuable resources for breeding and genetic studies of crop species. The development of simple sequence repeat (SSR) markers, which are applied across closely related cultivars, has been slow, labor-intensive, and expensive, with a low success rate. In this study, we demonstrate an efficient strategy for developing cross-species, amplifiable polymorphic SSR markers from grapevi ...
Vincenzo Landi; Emiliano Lasagna; Simone Ceccobelli; Amparo Martinez; Fatima Santos-Silva; Jose Luis Vega-Pla; Francesco Panella; Daniel Allain; Isabelle Palhiere; Maciej Murawski; Susana Dunner; Luìs Telo Da Gama; Cecilio Barba; Juan Vicente Delgado; Francesca Maria Sarti
... Although the Merino breed originated in the Iberian Peninsula, the Merino and Merino-derived sheep breeds have nowadays been widely distributed across the world both as purebreds and as admixed populations. In Europe, the Merino trunk has both high phenotypic and genetic diversity which originated from several genetic types adapted to different economic and farming environments. For these reasons, ...
... Turkey is a rich source of European hazelnut (Corylus avellana) germplasm with nearly 400 accessions in the national collection. This genetic material encompasses cultivars, landraces and wild genotypes which were characterized for 12 nut and 13 kernel traits over 2 years in the 1990s. Analysis of these attributes revealed both the positive and negative impacts that human selection and breeding ha ...
amplified fragment length polymorphism; apples; cultivars; genetic markers; genetic relationships; genetic variation; germplasm; microsatellite repeats; phylogeny; random amplified polymorphic DNA technique
Abstract:
... Knowledge of genetic relationship among genotypes is an important concern for utilization of germplasm resources in breeding. The need for knowledge in local apple cultivars is increasing and molecular marker analysis has contributed to understanding of the apple genetic diversity and phylogenetic relationship between genotypes. Three different DNA-based techniques, random amplified polymorphic DN ...
Styphnolobium japonicum; flowers; gene flow; genetic variation; heterozygosity; landraces; life history; microsatellite repeats; molecular biology; multidimensional scaling; population structure; trees; China
Abstract:
... Sophora japonica is a medium-sized deciduous native tree to China. Its flower buds and fruits have special medicinal value. By using 26 highly variable nuclear microsatellites, newly reported here, we assessed the genetic diversity and population structure of 10 representative populations (180 individuals in total) within the species. The results revealed a relatively high genetic diversity in S. ...
Pinus brutia var. eldarica; Rosa; Vitis; culture media; decline; discoloration; fungi; genetic variation; hybrids; leaves; microsatellite repeats; necrosis; pathogenicity; random amplified polymorphic DNA technique; stems; vascular tissues; vineyards; Iran
Abstract:
... Between March and September 2012, grapevines in vineyards of Meshginshahr county located in Ardabil province as well as Azarshahr, Ajab Shir, and Malekan counties located in East Azerbaijan province, north-western Iran represented general decline symptoms and in some cases black vascular discoloration. Annular or irregular discoloration, dark vascular streaking, and V-shaped unilateral canker or b ...
... Select cultivars of table olives have more desirable traits and a higher economic value. There are suspected issues with cultivar mislabeling and traceability in the supply chain. Here, we describe a method to identify cultivars by genotyping of processed olives. DNA was extracted from leaves and California-style olives of seven commonly packed cultivars. Processed olive fruits yielded relatively ...
... The aim of this study was to determine a possible association of IVS3 poly A and IVS10 CA microsatellites with CF in case–control Tunisian groups and to compare the results with the findings in Italian population. Both microsatellites were analyzed by capillary electrophoresis in 70 normal subjects and 60 Tunisian CF patients. The statistical distribution of IVS3 poly A and IVS10 CA polymorphism s ...
... Allelic variation in wild potato (Solanum) species was analysed using 14 simple sequence repeat (SSR) markers. SSR allelic profiles showed high polymorphism and distinctness among the wild species. A total of 109 alleles of 14 polymorphic SSR markers were scored in 82 accessions belonging to 22 wild potato species. Allele size ranged from a minimum of 104 bp (STI0030) to a maximum of 304 bp (STM51 ...
... BACKGROUND: Head formation of broccoli (Brassica oleracea var. italica) is greatly reduced under high temperature (22 °C and 27 °C). Broccoli inbred lines that are capable of producing heads at high temperatures in summer are varieties that are unique to Taiwan. However, knowledge of the early-activated pathways of broccoli head formation under high temperature is limited. RESULTS: We compared hea ...
... Algerian maize has been cultivated in Saharan Oases for many centuries, determining its adaption to extreme environments. Therefore, maize landraces from Sahara could be considered as valuable genetic resources for breeding. Morphological and molecular characterization of fifty-six populations were assessed using 14 agro-morphological traits and 18 SSR markers. Populations were evaluated in field ...
Oryza sativa; breeding programs; chromosome mapping; cluster analysis; coasts; genes; genetic markers; genetic relationships; genetic variation; genomics; landraces; loci; microsatellite repeats; population structure; quantitative trait loci; rice; salt tolerance; single nucleotide polymorphism; sodium; transporters; India
Abstract:
... Naturally evolved saline tolerant rice landraces found along the coastline of India are a valuable genomic resource to explore the complex, polygenic nature of salinity tolerance. In the present study, a set of 28 genome wide SSR markers, 11 salt responsive genic SSR markers and 8 Saltol QTL linked SSR markers were used to estimate genetic relatedness and population structure within a collection o ...
... We report on 14 microsatellites enriched in CT repeats obtained from a genomic library of lychee (Litchi chinensis Sonn.) cultivar “Hong Huay”. The polymorphisms revealed by these microsatellites were evaluated in a collection of 45 local Vietnamese lychee varieties and 4 Xerospermum noronhianum (Blume) Blume (Sapindaceae) collected from the wild. Samples were collected from local villages and for ...
... Increasingly, exogenous stressors such as pathogen infections, variable water conditions, and pollution are resulting in high mortality of Paphia undulata, deleteriously affecting the quality of clam harvests. The foot is a burrowing organ in clams. Physical damage and constant contact with the external environment cause the foot to be highly sensitive to pathogen invasion and water condition vari ...
... The genetic background of disorders of sex development (DSD) in dogs with a normal male sex chromosome set (78,XY) is poorly described. In this study, we present for the first time, an analysis of six genes of the testosterone pathway, encoding enzymes (CYP17A1, HSD3B2, HSD17B3, SRD5A2) and transcription factors (NR5A1, AR). The entire coding sequence and flanking regions of the introns, 5′‐UTR an ...
... KEY MESSAGE: The full-length transcriptome of alfalfa was analyzed with PacBio single-molecule long-read sequencing technology. The transcriptome data provided full-length sequences and gene isoforms of transcripts in alfalfa, which will improve genome annotation and enhance our understanding of the gene structure of alfalfa. As an important forage, alfalfa (Medicago sativa L.) is world-wide plant ...
... Taraxacum koksaghyz Rodin, a dandelion from the steppes of south-eastern Kazakhstan, has been known for long time as potential rubber producer, as a temperate region alternative to the tropical rubber tree Hevea brasiliensis Müll. Arg.. In this work, we evaluate Taraxacum bicorne Dahlst. (wild populations), a closely related congener of T. koksaghyz. The taxonomy of T. bicorne is reviewed, populat ...
... In this study, we performed transcriptome sequencing of D. similoides sinensis and developing of simple sequence repeats (SSRs) markers to identify valuable markers for Daphnia molecular genetics. The total length of D. similoides sinensis transcriptome sequence was 40 093 546 bp, which contained 12 017 SSR markers and 21 344 microsatellite loci. 2164 SSR primer pairs were obtained through design ...
Takifugu rubripes; Y chromosome; androgenesis; chromosome number; cold stress; diploidy; eggs; flow cytometry; fluorescence in situ hybridization; genotype; genotyping; gynogenesis; haploidy; inheritance (genetics); loci; microsatellite repeats; nucleolus organizer region; ribosomal DNA; sex determination; silver staining; single nucleotide polymorphism; triploidy
Abstract:
... Here, we explored cold‐shock‐induced haploid androgenesis in Japanese pufferfish (Takifugu rubripes) and analysed chromosomes by fluorescence in situ hybridization (FISH), single nucleotide polymorphism (SNP) in sex determination locus and microsatellite genotypes to verify all‐male inheritance. We also observed early embryonic development to have an insight into the cytological mechanism of cold‐ ...
... Wild germplasm with elevated antioxidants are a useful resource for using directly and in a breeding program. In a study with 136 wild clones and two cranberry cultivars, phenolic, flavonoid and anthocyanin contents varied 2.79, 2.70 and 17.46 times, respectively. The antioxidant activity ranged from 1.17 ± 0.01 to 2.53 ± 0.05 mg/g and varied 2.16 times. Seventy-five of wild clones and the cultiva ...
... Chrysanthemum is ranked second among the most economically important cut flowers after rose. This flower is considered very significant from different aspects of commercial herbs and traditional medicine. In this study, 30 simple sequence repeat (SSR) and 8 start codon targeted polymorphism (SCoT) markers were used to assess the genetic diversity of 32 cultivated varieties of C. morifolium. In tot ...
Oreochromis niloticus; alleles; automation; bioinformatics; data collection; ecological genetics; genetic markers; genetic variation; genotype; genotyping by sequencing; loci; microsatellite repeats; polymerase chain reaction; population genetics
Abstract:
... Microsatellites play an important role when investigating population and ecological genetics, although high effort in development and genotyping constitute a technical constraint and remains a major bottleneck. Here we use a microsatellite genotyping approach utilizing sequences of amplicons for allele calling (SSR-GBS) based on Illumina that requires less effort and time. The approach consist of ...
Chrysanthemum morifolium; alleles; cluster analysis; cultivars; genetic relationships; genetic variation; germplasm conservation; microsatellite repeats; ornamental plants; polymerase chain reaction; population structure; principal component analysis; provenance; China
Abstract:
... Chrysanthemum (Chrysanthemum morifolium), one of the most popular ornamentals throughout the world, has approximately 1600 years of cultivation history in China. In this study, 10 simple sequence repeat (SSR) markers were used to identify a collection of 88 chrysanthemum and its related genera accessions. In total, 42 effective alleles across 88 accessions were detected; 3429 bands were obtained b ...
... The need for improved aquaculture productivity has led to widespread pressure to introduce the Genetically Improved Farmed Tilapia (GIFT) strains of Nile tilapia (Oreochromis niloticus) into Africa. However, the physical and regulatory infrastructures for preventing the escape of farmed stocks into wild populations and ecosystems are generally lacking. This study characterized the genetic backgrou ...
... The general strategy for grapevine genetic resources conservation in Armenia encompasses the collection of the still existing diversity and the use of protection techniques to minimize the losses over time. Being studied mainly by ampelography, the genetic diversity of Armenian grapevine needs to be re-investigated in accordance with modern requirements and international scales. The purpose of the ...
Artemisia annua; Artemisia japonica; Artemisia vulgaris; DNA fingerprinting; Dracunculus; artemisinin; developmental stages; drying; flowers; genetic markers; genetic relationships; leaves; microsatellite repeats; random amplified polymorphic DNA technique; reversed-phase high performance liquid chromatography; solvents; temperature; tissues; toluene; India
Abstract:
... The low commercial production of artemisinin, the most potent anti-malarial compound, from leaves of Artemisia annua L. and other alternative methods are unable to fulfil its global needs. Therefore, to look for other potential sources, the present study investigated and compared artemisinin contents in aerial tissues of four Artemisia species of Manipur (viz., A. parviflora, A. vulgaris L., A. my ...
Piaractus mesopotamicus; alleles; aquaculture; breeding programs; breeding stock; fish farms; gene frequency; genetic structure; genetic variation; heterozygosity; inbreeding; kinship; microsatellite repeats; risk; rivers; single nucleotide polymorphism; tropical fish; variance; Brazil
Abstract:
... The pacu (Piaractus mesopotamicus) is a Neotropical fish with remarkable productive performance for aquaculture. Knowledge of genetic resources in Neotropical fish is essential for their applications in breeding programs. The aim of this study was to characterize the genetic diversity of seven farmed populations of pacu which will constitute the basis for a broodstock foundation for coming breedin ...
... This study investigated multiple mating in three species of intertidal klipfishes (family Clinidae): super klipfish Clinus superciliosus, bluntnose klipfish Clinus cottoides, and nosestripe klipfish Muraenoclinus dorsalis. These species display the rare reproductive mode of female vivipary with superfetation, a phenomenon where offspring at various stages of development are found within the female ...
... The decline of the eastern oyster (Crassostrea virginica) has prompted various restoration and aquaculture efforts. Recent field surveys in Rhode Island suggest that wild populations are increasing, yet the factors contributing to expansion are unknown. We used a population genetic approach to characterize genetic differences between wild and cultured oyster populations and explore the extent of c ...
... Short tandem repeats (STRs) are a widely utilized tool in forensic applications, the latter of which range from human identification and paternity testing to population analysis. The GlobalFiler STR loci, which includes 21 autosomal STRS, were analyzed in the Chechen subpopulation of Jordan. Whole blood samples were withdrawn from 159 Jordanian Chechen individuals, and genomic DNA was extracted fr ...
... BACKGROUND: Salinity is one of the most widespread abiotic stresses affecting rice productivity worldwide. The purpose of this study was to establish the relative importance of different traits associated with salinity tolerance in rice and to identify new quantitative trait loci (QTL) conferring tolerance to salinity at seedling stage. A total of 231 F₂:₃ plants derived from a cross between a sen ...
... Thousand cankers disease (TCD) results from the combined activity of the fungal pathogen, Geosmithia morbida Kolařík, Freeland, Utley, and Tisserat and its principle vector, Pityophthorus juglandis (Blackman) (Coleoptera: Curculionidae: Scolytinae) in Juglans L. spp. and Pterocarya Kunth spp. host plants. TCD has been reported from the eastern and western United States. To evaluate potential for o ...
Terminalia arjuna; Terminalia catappa; Terminalia chebula; fruits; genetic markers; genetic resources; genetic variation; leaves; microsatellite repeats; random amplified polymorphic DNA technique; species identification; trees
Abstract:
... Terminalia trees are being over exploited because of their medicinal and economical importance leading to loss of valuable genetic resources. The study included morphological variation and characterization of genetic diversity at interspecific level. Genus Terminalia is a complex group having many problems associated with taxonomic identification. Morphologically T. arjuna can be distinguished fro ...
... In the present paper we studied the genetic diversity and genetic structure of five Norway spruce (Picea abies (L.) Karsten) natural populations situated in Serbia, belonging to the southern lineage of the species at the southern margin of the species distribution range. Four populations occur as disjunct populations on the outskirts of the Dinaric Alps mountain chain, whereas one is located at th ...
... Raspberry is a valuable berry crop containing a large amount of antioxidants that correlates with the color of the berries. We evaluated the genetic diversity of differently colored raspberry cultivars by the microsatellite markers developed using the flavonoid biosynthesis structural and regulatory genes. Among nine tested markers, seven were polymorphic. In total, 26 alleles were found at seven ...
... The introgression of wild chromosomal segments into popular rice varieties is one of the potential approaches for developing varieties for drought stress condition. Sixteen genotypes, including nine indica, two tropical japonica and five chromosome segments substitution lines (CSSLs) with different levels of tolerance/susceptibility to drought stress, were selected for diversity study. Sixty-three ...
Camellia sinensis; alleles; beverages; cultivars; discriminant analysis; gene banks; genetic variation; germplasm; microsatellite repeats; new variety; plant breeding; population structure; tea; variance; South America; South Korea
Abstract:
... Tea (Camellia sinensis (L.) O. Kuntze) is cultivated in many developing Asian, African, and South American countries, and is the most widely consumed beverage in the world. It is of critical importance to understand the genetic diversity and population structure of tea germplasm for effective collection, conservation, and utilization. In this study, 410 tea accessions collected from South Korea we ...
... Iran is one of the main diversity centers and origins of pistachios in the world. Pistachio cultivation spread first within the ancient Persian Empire and then moved gradually westward. Knowledge of the genetic relationships among wild and cultivated varieties of pistachio is important for the efficient utilization of the available germplasm resources. Three molecular marker strategies, namely, in ...
... Hedychium coronarium is an important medicinal plant of Zingiberaceae family and is widely used in cosmetics industry and perfumery. H. coronarium is also the source of a number of valuable secondary metabolites including coronarin D, a compound with immense potential to treat cancer. Therefore, there is an urgent need to ensure sustainable use of this plant. The current study reports an one-step ...