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- Lubna Maryam; Shamsi Khalid; Abid Ali; Asad U. Khan
- RSC advances 2019 v.9 no.10 pp. 5325-5337
- Enterobacteriaceae; active sites; antibiotic resistance; arginine; asparagine; bacterial infections; beta-lactamase; cefotaxime; circular dichroism spectroscopy; fluorescence; hydrolysis; minimum inhibitory concentration; mutants; polymerase chain reaction; proteins; site-directed mutagenesis; spectral analysis
- ... Members of Enterobacteriaceae cause antibiotic-resistant infections worldwide. One such marker, CTX-M-15, expressed by Enterobacteriaceae produces β-lactamases, which hydrolyze the cephalosporin group of antibiotics, such as cefotaxime, used in the treatment of both Gram-positive and negative bacterial infections. Amino acid residues present in close proximity of the active site might also play a ...
- A.M. Somboro; J. Osei Sekyere; D.G. Amoako; H.M. Kumalo; R. Khan; L.A. Bester; S.Y. Essack
- Journal of applied microbiology 2019 v.126 no.2 pp. 452-467
- Enterobacteriaceae; active sites; adjuvants; beta-lactamase; binding capacity; carbapenems; cytotoxicity; enzyme inhibition; enzyme inhibitors; hydrophobicity; inhibitory concentration 50; minimum inhibitory concentration; tannins; transporters
- ... AIMS: We hypothesized and confirmed that tannic acid (TA) reverses carbapenem resistance by inhibiting carbapenemases in class A and B carbapenemase‐producing Enterobacteriaceae. METHODS AND RESULTS: Minimum inhibitory concentrations of carbapenems in the presence and absence of TA and other efflux pump inhibitors, TA‐carbapenemases inhibition assays and computational studies showed that TA had th ...