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... Drought stress is a serious threat to agriculture and the environment. Brassinosteroids (BRs) increase tolerance to drought stress of plant. Autophagy plays important roles in plant responses to drought stress; however, there are few reports on autophagy in peach (Prunus persica). In total, 23 putative autophagy-related genes (ATGs) in peach were identified using ATGs from the Arabidopsis thaliana ...
absorbance; bioassays; catalysts; cobalt; colorimetry; coordination polymers; detection limit; enzyme activity; enzyme kinetics; hot water treatment; hydrogen peroxide; manganese; peroxidase; surface area
Abstract:
... Metal–organic frameworks (MOFs) with a metal coordination structure mimicking that of enzymes have been widely applied in the sensing field for bioassays, and most of the reports focus on single-metal MOFs. However, bimetallic MOFs have fascinating catalytic capabilities due to their bimetallic coordination. Herein, a two-dimensional sheet-like bimetallic Co/Mn-based MOF (Co/Mn-MOF) with peroxidas ...
... The Fe₃O₄ nanozyme, the first reported nanozyme with intrinsic peroxidase-like activity, has been successfully employed for various diagnostic applications. However, only a few studies have been reported on the therapeutic applications of the Fe₃O₄ nanozyme partly due to its low affinity to the substrate H₂O₂. Herein, we report a new strategy for improving the peroxidase-like activity and affinity ...
... Peroxidase from Brassica rapa was immobilized as cross-linked enzyme aggregates (CLEAs) and used to treat air containing phenol as a model molecule of volatile organic compounds (VOCs). Prior to an enzymatic treatment, phenol was absorbed into an aqueous or organic phase (silicone oil) to reach concentrations ranging from 20 to 160 mg/L. The process was carried out by introducing a desired weighin ...
... Plants seeds are an important source of enzymes with biotechnological interest. In particular, plant peroxidases, because of their enzymatic activity and stability, are used for the synthesis of phenolic resins, in the treatment of waste waters, or as labelling enzymes and in food industry. Thus, these enzymes can give a potential substitute of other polluting industrial catalysts to the conservat ...
... Following an inspiration from the chemistry of protein nanocages in nature, we evaluated the performance of a hemin-functionalized GroEL nanocage as peroxidase mimics for chromogenic detection. The reactivity of this biohybrid formed by detergent dialysis was found to be highly dependent on the amount of hemin encapsulated inside the GroEL central cavity. In comparison with horseradish peroxidase ...
cadmium; chemical reactions; chemical structure; enzyme activity; heavy metals; microwave radiation; microwave treatment; organic compounds; peroxidase
Abstract:
... A facile and efficient approach to N-(2-nitrophenyl)benzenesulfonamides was developed under microwave irradiation. A series of pyrabactin analogues containing nitrophenyl scaffold was obtained in excellent yields. In addition, the method was pretty suitable to prepare flusulfamide. Significantly, the 3ae could enhance POD activity in response to heavy metal stress. ...
Fourier transform infrared spectroscopy; ambient temperature; analytical chemistry; biomimetics; coatings; dicarboxylic acids; dopamine; energy conservation; hydrogen peroxide; infrared radiation; magnetic materials; methanol; nanoparticles; peroxidase; photostability; polymers; solvents; transmission electron microscopes; transmission electron microscopy; ultraviolet-visible spectroscopy
Abstract:
... In this paper, ferrocene derivative polymer nanoparticles (FcP NPs) with uniform size and good photostability was synthesized using 1,1′-ferrocene dicarboxylic acid as precursor and methanol as solvent. FcP NPs-PDA was further obtained by coating of polydopamine (PDA) on FcP NPs in tris-HCl (pH=8.5) buffer solution at room temperature in the presence of dopamine (DA). The structure and morphology ...
Commiphora gileadensis; Fourier transform infrared spectroscopy; ammonium sulfate; durability; gel chromatography; immobilized enzymes; industrial applications; ion exchange chromatography; magnetism; pH; peroxidase; scanning electron microscopy; stems; storage temperature; thermal stability
Abstract:
... A novel plant peroxidase was isolated from the stem of Arabian balsam (Commiphora gileadensis) and purified using ammonium sulfate, followed by ion exchange chromatography (DEAE-Sepharose) and gel filtration (Sephcryl S-200). The newly isolated peroxidase was characterized as having a specific activity of 9503.3 unit/mg of protein after 20.3-fold purification, which yielded a recovery of 18.5%. Ba ...
Nisha Sharma; Giorgio Arrigoni; Leonard Barnabas Ebinezer; Anna Rita Trentin; Cinzia Franchin; Sabrina Giaretta; Paolo Carletti; Sören Thiele-Bruhn; Rossella Ghisi; Antonio Masi
... Animal manure or bio-solids used as fertilizers are the main routes of antibiotic exposure in the agricultural land, which can have immense detrimental effects on plants. Sulfadiazine (SDZ), belonging to the class of sulfonamides, is one of the most detected antibiotics in the agricultural soil. In this study, the effect of SDZ on the growth, changes in antioxidant metabolite content and enzyme ac ...
... In this work, an ultrasensitive aptasensor for the detection of Mucin 1 (MUC1) was presented based on the target-induced catalytic hairpin assembly combined with excellent mimic peroxidase performance of PtPd bimetallic nanoparticles (PtPdNPs). Traditionally, the cyclic reuse of target protein was achieved by protein conversion with enzyme cleavage or polymerization, which is costly and complex. H ...
... Saxitoxin (STX) has high toxicity, and is water soluble, acid stable and thermostable. Therefore, STX in seawater can be accumulated by marine organism to form bioaccumulation. To ensure the safety of seafood for consumption, it is crucial to accurately determine trace STX in seawater and seafood. We herein developed a novel magnetic electrochemical immunosensor for ultra-sensitive detection of ST ...
... Horseradish peroxidase (HRP) isoenzyme C1a is one of the most widely used enzymes for various analytical methods in bioscience research and medical fields. In these fields, real-time monitoring of HRP activity is highly desirable because the utility of HRP as a reporter enzyme would be expanded. In this study, we developed a simple assay system enabling real-time monitoring of HRP activity by usin ...
... The objective of the present study was to investigate the effectiveness of the post-harvest treatments of abiotic elicitors, that is, calcium chloride (CaCl₂) and salicylic acid (SA) on physicochemical and biochemical parameters in relation to activities of antioxidative enzymes in carrot to enhance shelf life. Carrot of variety Punjab Carrot Red was harvested, washed, surface dried and treated wi ...
... Di(2-ethylhexyl) phthalate (DEHP) and di(n-butyl) phthalate (DBP) are the major phthalic acid esters to be used during the plastic manufacturing process; they have emerged as pollutants that result in serious environmental problems. However, their impacts on wheat at the reproductive stage remain unclear. Here, we examined the distribution of DEHP and DBP and their respective metabolites mono(2-et ...
Larix sibirica; air pollution; cadmium; carotenoids; chlorophyll; chromium; cities; cobalt; copper; enzyme activity; heavy metals; highways; iron; land use; lead; manganese; microclimate; nickel; peroxidase; pigments; pollution control; proanthocyanidins; proline; soil pollution; tannins; traffic; urban areas; urbanization; zinc; Russia
Abstract:
... Active urbanization processes exacerbate environmental problems associated with industrial pollution in cities. Urban greening helps reduce level of air pollution and improve microclimate. Selection sensitive plant species (indicators of pollution), and the resistant species (decrease the level pollution) is acute in many countries. The aim of the present work was to establish concentrations of he ...
... Food endogenous enzymes have impacts on color, texture and flavor of foods during food processing or preservation. Cold plasma is a novel non-thermal food processing technology, which has been extensively studied for contamination elimination and shelf life extension of foods. Particularly, much work has been reported about the effects of cold plasma on enzyme activities and alterations about enzy ...
... White mold of common bean caused by Sclerotinia sclerotiorum is a serious disease in temperate and subtropical regions. The present study evaluated the effects of culture filtrates of Purpureocillium lilacinum on S. sclerotiorum under in vitro and in vivo conditions at the concentration of 75% of culture filtrates. The mycelial growth, sclerotia formation, myceliogenic and carpogenic germination o ...
... Azoxystrobin is a strobilurin of growing concern in aquatic environments because it is the most sold fungicide worldwide, however, the information available about its effect on aquatic non-target organisms is scarce. The objective of the present study was to evaluate potential physiological, biochemical, and genetic effects at environmentally relevant (1–10 μg/L) and elevated (100–500 μg/L) concen ...
... Triflumizole is one of imidazole fungicides that works by inhibiting ergosterol biosynthesis, and is widely used for the control of powdery mildew and scabs on various fruits and crops. Triflumizole residue has been frequently detected in soil and aquatic ecosystems. While many studies have focused on its toxic effect on terrestrial and aquatic animals, little attention has been paid to aquatic al ...
... Triflumuron (TFM) is one of the most widely used insecticides over the world. It is a benzoylphenyl urea that belongs to the class of insect growth regulators. This insecticide acts by inhibiting insect’s chitin synthesis and by consequences, making insect more susceptible to pathogens and malformations. TFM effects have been reported in mammalians and crops. However, studies that reveal its toxic ...
... Bridging-induced aggregation of individual nanozyme particles by long-chain biomacromolecules causes loss of peroxidase mimetic activity for various nanozymes. When a common nanozyme (Fe3O4 nanoparticles) was used for colorimetric assay of a long-chain biomacromolecule, human genomic DNA (hgDNA, 14.000 kDa, containing 22 kilobases), peroxidase-like activity was absent owing to the irreversible agg ...
... Concentration-dependent responses of cucumber plants to cadmium (Cd, 5–15 µM) and/or allantoin (Alla, 10–1000 µM) have been investigated to detect a possible protective role of Alla under Cd excess. After 14 days of exposure, Alla often considerably reversed Cd-induced inhibition of growth and reduction of the content of photosynthetic pigments. Higher Alla doses depleted the Cd amount in shoots, ...
... Lichens are globally widespread organisms playing an important role in diverse ecosystems. They produce secondary metabolites, unique compounds, which play many important ecological and biological roles, including their effects on other plants, through allelopathy. Usnic acid is one of the most frequent secondary compounds in thalli of lichens forming the layer on the surface of soils, interacting ...
... Alleviation of cadmium-induced root genotoxicity and cytotoxicity by calcium chloride (CaCl₂) in faba bean (Vicia faba L. var. minor) seedlings were studied. Faba bean seeds were treated with H₂O or 2% CaCl₂ for 6 h before germination. Seeds were then exposed to 0 and 50 µM CdCl₂ concentrations for 7 days. Genotoxic damaging effects of Cd was examined through the determination of the mitotic index ...
... Chromium is a key metal pollutant of the environment. When present in high concentrations in soil it adversely affects plant growth and productivity. Phyllanthus emblica(P.emblica) aqueous extract contains many natural antioxidants, and it has been widely used in traditional medicine for the treatment of severe oxidative stress-related diseases and disorders. The present study was aimed to investi ...
... The role of exogenous individual or combined application of silicon (Si) and salicylic acid (SA) (control, 6mm Si, 1mm SA, and 6mm Si+1mm SA) on grain yield and some key physiological characteristics of wheat (Triticum aestivum L.) cvv. Shiraz (drought-sensitive) and Sirvan (drought-tolerant) was investigated under field water-stress conditions (100% and 40% field capacity). Drought stress caused ...
... BACKGROUND: Salinity has been a major abiotic stressor that reduce the productivity. Previous studies reported that endophytic bacteria produce plant stress response hormones, antioxidants, and enzymes such as ACC deaminase. Augmentation of these metabolites and enzymes by endophytes mitigates the stress effects of salinity and improves plant growth and productivity. METHODS: Bacterial endophytes ...
... The present investigation describes aluminum-induced changes in the leaves of two buckwheat species using both physiological and biochemical indices. With increasing levels of Al (viz. 100, 200 and 300 μM), the mean length of root, shoot as well as their biomass accumulation decreased linearly with respect to control. Tolerance test of F. kashmirianum revealed that it was more tolerant to Al-stres ...
... Pistachio is of high sensitivity to such physiological disorders as fruit abscission, endocarp lesion nut, blank, deformed, early split and non-split nuts. However, little research has been carried out on the physiological disorders in pistachio. In this study, the effects of organic manure, gypsum and boric acid treatments with their combination were studied on physiological disorders in ‘Kaleh-G ...
... The present research investigates the interactive effects of 24-epibrassinolide (0, 0.02 and 0.5 µM) plus thiamine (0 and 200 µM) treatments on some of physio-biochemical parameters in Brassica napus plants under Cadmium Chloride toxic levels; 250 and 500 µM. Application of 24-epibrassinolide 0.02 µM plus thiamine 200 µM significantly increased growth and biochemical parameters such as shoot lengt ...
... In this report, a novel nanocomposite of highly dispersed Au nanoparticles deposited on NH₂-MIL-125(Ti) was designed and proposed as a peroxidase-like mimic. Compared with individual Au nanoparticles and NH₂-MIL-125(Ti), the peroxidase-like nanozyme exhibits enhanced peroxidase-like activity. Moreover, kinetic analysis reveals that the as-prepared nanozyme has lower Kₘ values and stronger affinity ...
... Prolific and rapid in vitro plant organogenesis via direct regeneration has been obtained from axenic seedling-derived petiole and leaf explants of Ficus religiosa in Murashige and Skoog (MS) medium containing different concentrations of cytokinins in combination with indole-3-butyric acid (IBA). MS medium with 1.5 mg/l 6-benzylaminopurine plus 0.15 mg/l IBA produced the highest shoot induction fr ...
... Human toxocariasis is a zoonosis resulting from the migration of larval stages of the dog parasite Toxocara canis into the human paratenic host. Despite its well-known limitations, serology remains the most important tool to diagnose the disease. Our objective was to employ camelid single domain antibody fragments also known as nanobodies (Nbs) for a specific and sensitive detection of Toxocara ca ...
... In this study, we established an ultrasensitive electrochemical immunosensor based on the gold nanoparticles-enhanced tyramide signal amplification (AuNPs-TSA) for the detection of procalcitonin (PCT, for discriminating bacterial infections from nonbacterial infections). Firstly, a facilely prepared, well-conducting reduced graphene oxide nanosheets/GNP (rGO-AuNPs) nanocomposite was synthesized an ...
... A diverse pool of 310 bacterial endophytes isolated from tomato plants growing in the Indo-Gangetic plains of India was evaluated for their antagonistic potential against three soil-borne fungal pathogens causing root rot (Rhizoctonia solani), collar rot (Sclerotium rolfsii) and, fungal wilt (Fusarium oxysporum f. sp. lycopersici). Preliminary screening indicated that most of the isolates could in ...
... Terrestrial ecosystems in the Northern Hemisphere are a globally important sink for anthropogenic CO₂ in the Earth's atmosphere, slowing its accumulation as well as the pace of climate warming. With the use of a long‐term field experiment (ca. 20 yr), we show that the expression of fungal class II peroxidase genes, which encode enzymes mediating the rate‐limiting step of organic matter decay, are ...
... AMP-activated protein kinase (AMPK) is a serine/threonine protein kinase critically involved in the regulation of cellular energy homeostasis. Many studies have suggested that AMPK activation exert significant anti-inflammatory effects. In this study, we assessed the anti-inflammatory effect of AMPK activator from natural products, Broccoli seed (BS, Brassica oleracea var. italic) extract and Swee ...
... In this work the polymerization of catechin, epicatechin, and resveratrol was carried out through a peroxidase oxidation process in order to improve the biological activity of these phenolic compounds. The antioxidant activity of the oligomers was evaluated by their ability to scavenge reactive oxygen species (ROS) and their capacity to chelate metal ions Fe2+ and Cu2+. The antitumor effect of the ...
... A pot experiment was conducted to study antioxidant enzyme and biochemical behaviour of two cultivars of chickpea (Cicer arietinum L.) viz., BG-256 (salt sensitive) and CSG-8962 (salt tolerant)] under salt-stress conditions. Salt stress was imposed by applying 50, 100 and 150 mM NaCl to plants as per treatment. Untreated plants were maintained as control. Plant samples were analyzed from 20 DAS up ...
... The effect of lead (Pb)-induced oxidative stress was investigated in Fagopyrum kashmirianum. The seedlings absorbed the Pb readily by showing time (15 and 30 days) and concentration (0, 100, 200 and 300 μM) dependent effects. Pb caused reduction in both root and shoot lengths but its accumulation was more in roots (22.32 mg g−1 DW) than shoots (8.86 mg g−1 DW) at the highest concentration (300 μM) ...
... The responses of the cyanobacteria Nostoc ellipsosporum and Nostoc piscinale to salt stress during various growth stages were studied. Nostoc ellipsosporum was more NaCl tolerant and attained more biomass under salinity than N. piscinale. Accumulation of proline was detected in N. ellipsosporum under salinity stress. Malondialdehyde content decreased in both species on day 9 (mid log phase) under ...
... As a broad-spectrum herbicide, glyphosate was extensively utilised in China for several decades. The contradiction between glyphosate spraying and crab breeding in the rice-crab co-culture system has become more obvious. In this study, the antioxidative status and immunological responses of Chinese mitten crab, Eriocheir sinensis, under sublethal exposure of glyphosate were investigated by detecti ...
... Drought is one of the major environmental constrains that limit plant performance worldwide. Plant apoplast which acts as connecting link between environment and plant protoplast carries multiple functions in plant metabolism and signalling. To investigate the drought induced changes in apoplast, proteome analysis in conjunction with antioxidant enzyme activity changes were studied in chilli (Caps ...
... Livestock and poultry manure containing high levels of copper and zinc have led to contamination of farmland and products which could have an impact on human health. Biochar is an inexpensive and efficient heavy metal absorbent. In the present study, we have used biochar to mitigate the effects of heavy metals on the growth of wheat seedlings. The study showed that the effects of heavy metals on w ...
... Plant biostimulants (PBS) increase crop productivity and induce beneficial processes in plants. Although PBS can stimulate plant tolerance to some abiotic stresses, their effect in improving crop resistance to herbicide injuries has barely been investigated. Therefore, a study on the effect of a biostimulant (Megafol) on maize (Zea mays L.) tolerance to a chloro-acetanilide herbicide (metolachlor) ...
biosensors; chitosan; electrical conductivity; electron transfer; glassy carbon electrode; glucose; glucose oxidase; immobilized enzymes; nanofibers; peroxidase; polymerization; polymers; scanning electron microscopy; surface area; tin; tin dioxide
Abstract:
... This work reports on a novel glucose biosensor based on co-immobilization of glucose oxidase (GOx) and horseradish peroxidase with polymerized multiporous nanofiber (MPNFs) of SnO₂ onto glassy carbon electrode with chitosan. Multiporous nanofibers of SnO₂ were synthesized by electrospinning method from the tin precursor which possesses high surface area good electrical conductivity, and the nanofi ...
... Herein, high performance peroxidase-like activity of zinc and cobalt bi-metal metal-organic framework (ZnCo MOF) is reported and applied for the sensitive measurement of atropine. ZnCo MOF was synthesized by the reaction of 2-methylimidazole with Zn and Co (II) cations in aqueous media. The colorimetric and fluorometric experiments were applied to investigate the catalytic activity of obtained MOF ...
... KEY MESSAGE: Arabidopsis GI negatively regulates chloroplast biogenesis and resistance to the herbicide butafenacil by enhanced activity and transcriptional levels of antioxidant enzymes Chloroplast biogenesis is blocked by retrograde signaling triggered by diverse internal and external cues, including sugar, reactive oxygen species (ROS), phytohormones, and abiotic stress. Efficient chloroplast b ...
... Arbuscular mycorrhizal (AM) fungi (AMF) are widely known to form a symbiosis with most higher plants and enhance plant adaptation to a series of environmental stresses. Sweet sorghum (Sorghum bicolor (L.) Moench) is considered a promising alternative feedstock for bioalcohol production because of its sugar-rich stalk and high biomass. However, little is known of AMF benefit for biomass production ...